AlfalfaGEDB | MS.gene01768

M. sativa cultivar XinJiangDaYe / MS.gene01768

KEGG: K02898
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0015934 GO:0019538 GO:0032991 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044391 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576 GO:1990904
PFAM: PF00467.32 PF16906.8

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene01768.t1	XP_003593461.1	100	146	0	0	1	146	1	146	1.40E-71	278.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene01768.t1	Q9FJX2	88.4	146	17	0	1	146	1	146	5.7e-66	251.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene01768.t1	G7ILF2	100.0	146	0	0	1	146	1	146	9.9e-72	278.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene01768	MS.gene002382	PPI
MS.gene01768	MS.gene015255	PPI
MS.gene01768	MS.gene43544	PPI
MS.gene01360	MS.gene01768	PPI
MS.gene20295	MS.gene01768	PPI
MS.gene01768	MS.gene047519	PPI
MS.gene01768	MS.gene09597	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene01768.t1	MTR_2g012450	100.000	146	0	0	1	146	1	146	1.04e-103	293
MS.gene01768.t1	MTR_5g015570	95.205	146	7	0	1	146	1	146	2.04e-90	259
MS.gene01768.t1	MTR_5g096540	91.096	146	13	0	1	146	1	146	4.27e-87	251
MS.gene01768.t1	MTR_4g126220	88.235	68	8	0	13	80	103	170	6.53e-38	127
MS.gene01768.t1	MTR_8g073140	56.977	86	10	1	30	115	1	59	2.66e-23	87.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene01768.t1	AT5G67510	88.356	146	17	0	1	146	1	146	9.99e-93	265
MS.gene01768.t1	AT3G49910	85.616	146	21	0	1	146	1	146	1.18e-91	262

Find 49 sgRNAs with CRISPR-Local

Find 47 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGTTGGTGTTAATCCTTCTA+AGG	0.281571	2.3:+71625064	MS.gene01768:CDS
TATTATGCAGACCGTTGATT+AGG	0.363868	2.3:+71625199	MS.gene01768:CDS
CATTGAACGGACGTTGTACT+TGG	0.377101	2.3:-71624898	None:intergenic
TTAGTGATAACAACCTTAGA+AGG	0.401319	2.3:-71625077	None:intergenic
GGGTCGTGCTGCTGCTGATA+AGG	0.420559	2.3:+71625148	MS.gene01768:CDS
TCGCAAGGACGACGAAGTTC+AGG	0.422063	2.3:+71624926	MS.gene01768:CDS
ACGGCTCTTCCTACGGCTGC+TGG	0.422834	2.3:-71624802	None:intergenic
AAGACACGGCGGACGCTTGA+TGG	0.437229	2.3:-71624843	None:intergenic
CACTAAGCTCAGATTGGATA+AGG	0.438891	2.3:+71625094	MS.gene01768:CDS
CCTGAGTAACCTTACCTTCA+CGG	0.446679	2.3:-71624971	None:intergenic
ACTTCGTCGTCCTTGCGAAC+AGG	0.458496	2.3:-71624921	None:intergenic
TGTTATCACTAAGCTCAGAT+TGG	0.466490	2.3:+71625088	MS.gene01768:CDS
AGGATTAACACCAACATTAA+CGG	0.467585	2.3:-71625057	None:intergenic
ATTCAAAGGCCGTGAAGGTA+AGG	0.498862	2.3:+71624962	MS.gene01768:CDS
GCTACTTGATCGTAAGGCTA+AGG	0.528270	2.3:+71625127	MS.gene01768:CDS
CTACGGCTGCTGGAAACTCG+TGG	0.530535	2.3:-71624792	None:intergenic
GACGAAGTTCAGGTCGTTCG+TGG	0.531110	2.3:+71624936	MS.gene01768:CDS
CTCAGGTGTACCGTCGCAAG+TGG	0.531799	2.3:+71624988	MS.gene01768:CDS
TGCTGCTGCTGATAAGGAGA+AGG	0.537077	2.3:+71625154	MS.gene01768:CDS
GAGCCGTAAGGCTCATTTCA+CGG	0.545137	2.3:+71624818	MS.gene01768:CDS
ATCACAAGGGAGAAGGTGAA+TGG	0.545267	2.3:+71625029	MS.gene01768:CDS
CCGTTCAATGCCTGTTCGCA+AGG	0.545521	2.3:+71624911	MS.gene01768:CDS
GAGCCTTACGGCTCTTCCTA+CGG	0.547173	2.3:-71624809	None:intergenic
CTACTTGATCGTAAGGCTAA+GGG	0.553374	2.3:+71625128	MS.gene01768:CDS
GTCGTTCGTGGAACATTCAA+AGG	0.554383	2.3:+71624948	MS.gene01768:CDS
TAAATCGCTACTTGATCGTA+AGG	0.555125	2.3:+71625121	MS.gene01768:CDS
GGGTACTAAGTTTGCTCCTG+AGG	0.563912	2.3:+71625175	MS.gene01768:CDS
ACGGTCTGCATAATATCCTC+AGG	0.575139	2.3:-71625191	None:intergenic
GCGCCGTGAAATGAGCCTTA+CGG	0.575259	2.3:-71624821	None:intergenic
CGAACGCATCACAAGGGAGA+AGG	0.580140	2.3:+71625022	MS.gene01768:CDS
GATCGCAGATCGGCGGAGAG+TGG	0.584970	2.3:-71624876	None:intergenic
TACGGCTGCTGGAAACTCGT+GGG	0.586325	2.3:-71624791	None:intergenic
CCGTGAAGGTAAGGTTACTC+AGG	0.586540	2.3:+71624971	MS.gene01768:CDS
TTCACATCGAACGCATCACA+AGG	0.588472	2.3:+71625015	MS.gene01768:CDS
CAGCCGTAGGAAGAGCCGTA+AGG	0.591177	2.3:+71624806	MS.gene01768:CDS
GTTGTACTTGGATCGCAGAT+CGG	0.604312	2.3:-71624886	None:intergenic
GGAACATTCAAAGGCCGTGA+AGG	0.606062	2.3:+71624957	MS.gene01768:CDS
CCTTGCGAACAGGCATTGAA+CGG	0.606739	2.3:-71624911	None:intergenic
GATTCAAGAATCCTAATCAA+CGG	0.625900	2.3:-71625210	None:intergenic
AATGGATCCACCGTTAATGT+TGG	0.632764	2.3:+71625047	MS.gene01768:CDS
GCTGCTGCTGATAAGGAGAA+GGG	0.634378	2.3:+71625155	MS.gene01768:CDS
ATTAACACCAACATTAACGG+TGG	0.662702	2.3:-71625054	None:intergenic
TGTGAATCACCCACTTGCGA+CGG	0.675653	2.3:-71624998	None:intergenic
TCAGGTGTACCGTCGCAAGT+GGG	0.677048	2.3:+71624989	MS.gene01768:CDS
CACGAGTTTCCAGCAGCCGT+AGG	0.686122	2.3:+71624793	MS.gene01768:CDS
GTACTTGGATCGCAGATCGG+CGG	0.688339	2.3:-71624883	None:intergenic
GTGGCGCGCTCATTAAGACA+CGG	0.705562	2.3:-71624857	None:intergenic
TCACATCGAACGCATCACAA+GGG	0.710980	2.3:+71625016	MS.gene01768:CDS
GCGCGCTCATTAAGACACGG+CGG	0.823768	2.3:-71624854	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AGGATTAACACCAACATTAA+CGG	-	chr2.3:71625060-71625079	None:intergenic	30.0%
	TTAGTGATAACAACCTTAGA+AGG	-	chr2.3:71625080-71625099	None:intergenic	30.0%
	ATTAACACCAACATTAACGG+TGG	-	chr2.3:71625057-71625076	None:intergenic	35.0%
	TAAATCGCTACTTGATCGTA+AGG	+	chr2.3:71625121-71625140	MS.gene01768:CDS	35.0%
	TGTTATCACTAAGCTCAGAT+TGG	+	chr2.3:71625088-71625107	MS.gene01768:CDS	35.0%
!!	TGTTGGTGTTAATCCTTCTA+AGG	+	chr2.3:71625064-71625083	MS.gene01768:CDS	35.0%
	AATGGATCCACCGTTAATGT+TGG	+	chr2.3:71625047-71625066	MS.gene01768:CDS	40.0%
	CACTAAGCTCAGATTGGATA+AGG	+	chr2.3:71625094-71625113	MS.gene01768:CDS	40.0%
	CTACTTGATCGTAAGGCTAA+GGG	+	chr2.3:71625128-71625147	MS.gene01768:CDS	40.0%
	ACGGTCTGCATAATATCCTC+AGG	-	chr2.3:71625194-71625213	None:intergenic	45.0%
	ATCACAAGGGAGAAGGTGAA+TGG	+	chr2.3:71625029-71625048	MS.gene01768:CDS	45.0%
	ATTCAAAGGCCGTGAAGGTA+AGG	+	chr2.3:71624962-71624981	MS.gene01768:CDS	45.0%
	CATTGAACGGACGTTGTACT+TGG	-	chr2.3:71624901-71624920	None:intergenic	45.0%
	CCTGAGTAACCTTACCTTCA+CGG	-	chr2.3:71624974-71624993	None:intergenic	45.0%
	GCTACTTGATCGTAAGGCTA+AGG	+	chr2.3:71625127-71625146	MS.gene01768:CDS	45.0%
	GTCGTTCGTGGAACATTCAA+AGG	+	chr2.3:71624948-71624967	MS.gene01768:CDS	45.0%
	GTTGTACTTGGATCGCAGAT+CGG	-	chr2.3:71624889-71624908	None:intergenic	45.0%
	TCACATCGAACGCATCACAA+GGG	+	chr2.3:71625016-71625035	MS.gene01768:CDS	45.0%
	TTCACATCGAACGCATCACA+AGG	+	chr2.3:71625015-71625034	MS.gene01768:CDS	45.0%
	CCGTGAAGGTAAGGTTACTC+AGG	+	chr2.3:71624971-71624990	MS.gene01768:CDS	50.0%
	GAGCCGTAAGGCTCATTTCA+CGG	+	chr2.3:71624818-71624837	MS.gene01768:CDS	50.0%
	GCTGCTGCTGATAAGGAGAA+GGG	+	chr2.3:71625155-71625174	MS.gene01768:CDS	50.0%
	GGAACATTCAAAGGCCGTGA+AGG	+	chr2.3:71624957-71624976	MS.gene01768:CDS	50.0%
	TGCTGCTGCTGATAAGGAGA+AGG	+	chr2.3:71625154-71625173	MS.gene01768:CDS	50.0%
	TGTGAATCACCCACTTGCGA+CGG	-	chr2.3:71625001-71625020	None:intergenic	50.0%
!	GGGTACTAAGTTTGCTCCTG+AGG	+	chr2.3:71625175-71625194	MS.gene01768:CDS	50.0%
!!	CCTTGCGAACAGGCATTGAA+CGG	-	chr2.3:71624914-71624933	None:intergenic	50.0%
	ACTTCGTCGTCCTTGCGAAC+AGG	-	chr2.3:71624924-71624943	None:intergenic	55.0%
	CCGTTCAATGCCTGTTCGCA+AGG	+	chr2.3:71624911-71624930	MS.gene01768:CDS	55.0%
	CGAACGCATCACAAGGGAGA+AGG	+	chr2.3:71625022-71625041	MS.gene01768:CDS	55.0%
	GACGAAGTTCAGGTCGTTCG+TGG	+	chr2.3:71624936-71624955	MS.gene01768:CDS	55.0%
	GAGCCTTACGGCTCTTCCTA+CGG	-	chr2.3:71624812-71624831	None:intergenic	55.0%
	GCGCCGTGAAATGAGCCTTA+CGG	-	chr2.3:71624824-71624843	None:intergenic	55.0%
	GTACTTGGATCGCAGATCGG+CGG	-	chr2.3:71624886-71624905	None:intergenic	55.0%
	GTGGCGCGCTCATTAAGACA+CGG	-	chr2.3:71624860-71624879	None:intergenic	55.0%
	TACGGCTGCTGGAAACTCGT+GGG	-	chr2.3:71624794-71624813	None:intergenic	55.0%
	TCAGGTGTACCGTCGCAAGT+GGG	+	chr2.3:71624989-71625008	MS.gene01768:CDS	55.0%
	TCGCAAGGACGACGAAGTTC+AGG	+	chr2.3:71624926-71624945	MS.gene01768:CDS	55.0%
	AAGACACGGCGGACGCTTGA+TGG	-	chr2.3:71624846-71624865	None:intergenic	60.0%
	CACGAGTTTCCAGCAGCCGT+AGG	+	chr2.3:71624793-71624812	MS.gene01768:CDS	60.0%
	CAGCCGTAGGAAGAGCCGTA+AGG	+	chr2.3:71624806-71624825	MS.gene01768:CDS	60.0%
	CTACGGCTGCTGGAAACTCG+TGG	-	chr2.3:71624795-71624814	None:intergenic	60.0%
	CTCAGGTGTACCGTCGCAAG+TGG	+	chr2.3:71624988-71625007	MS.gene01768:CDS	60.0%
	GCGCGCTCATTAAGACACGG+CGG	-	chr2.3:71624857-71624876	None:intergenic	60.0%
!	GGGTCGTGCTGCTGCTGATA+AGG	+	chr2.3:71625148-71625167	MS.gene01768:CDS	60.0%
	ACGGCTCTTCCTACGGCTGC+TGG	-	chr2.3:71624805-71624824	None:intergenic	65.0%
	GATCGCAGATCGGCGGAGAG+TGG	-	chr2.3:71624879-71624898	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.3	gene	71624780	71625220	71624780	ID=MS.gene01768
chr2.3	mRNA	71624780	71625220	71624780	ID=MS.gene01768.t1;Parent=MS.gene01768
chr2.3	exon	71624780	71625220	71624780	ID=MS.gene01768.t1.exon1;Parent=MS.gene01768.t1
chr2.3	CDS	71624780	71625220	71624780	ID=cds.MS.gene01768.t1;Parent=MS.gene01768.t1

Gene Sequence

>MS.gene01768

ATGAAGTTCAACCCACGAGTTTCCAGCAGCCGTAGGAAGAGCCGTAAGGCTCATTTCACGGCGCCATCAAGCGTCCGCCGTGTCTTAATGAGCGCGCCACTCTCCGCCGATCTGCGATCCAAGTACAACGTCCGTTCAATGCCTGTTCGCAAGGACGACGAAGTTCAGGTCGTTCGTGGAACATTCAAAGGCCGTGAAGGTAAGGTTACTCAGGTGTACCGTCGCAAGTGGGTGATTCACATCGAACGCATCACAAGGGAGAAGGTGAATGGATCCACCGTTAATGTTGGTGTTAATCCTTCTAAGGTTGTTATCACTAAGCTCAGATTGGATAAGGATCGTAAATCGCTACTTGATCGTAAGGCTAAGGGTCGTGCTGCTGCTGATAAGGAGAAGGGTACTAAGTTTGCTCCTGAGGATATTATGCAGACCGTTGATTAG

Protein sequence

>MS.gene01768.t1

MKFNPRVSSSRRKSRKAHFTAPSSVRRVLMSAPLSADLRSKYNVRSMPVRKDDEVQVVRGTFKGREGKVTQVYRRKWVIHIERITREKVNGSTVNVGVNPSKVVITKLRLDKDRKSLLDRKAKGRAAADKEKGTKFAPEDIMQTVD