AlfalfaGEDB | MS.gene047519

M. sativa cultivar XinJiangDaYe / MS.gene047519

KEGG: K02898
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0015934 GO:0019538 GO:0032991 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044391 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576 GO:1990904
PFAM: PF00467.32 PF16906.8

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene047519.t1	XP_003593461.1	100	146	0	0	1	146	1	146	1.40E-71	278.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene047519.t1	Q9FJX2	88.4	146	17	0	1	146	1	146	5.7e-66	251.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene047519.t1	G7ILF2	100.0	146	0	0	1	146	1	146	9.9e-72	278.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene047519	MS.gene015255	PPI
MS.gene047519	MS.gene43544	PPI
MS.gene01360	MS.gene047519	PPI
MS.gene20295	MS.gene047519	PPI
MS.gene047519	MS.gene09597	PPI
MS.gene047519	MS.gene002382	PPI
MS.gene01768	MS.gene047519	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene047519.t1	MTR_2g012450	100.000	146	0	0	1	146	1	146	1.04e-103	293
MS.gene047519.t1	MTR_5g015570	95.205	146	7	0	1	146	1	146	2.04e-90	259
MS.gene047519.t1	MTR_5g096540	91.096	146	13	0	1	146	1	146	4.27e-87	251
MS.gene047519.t1	MTR_4g126220	88.235	68	8	0	13	80	103	170	6.53e-38	127
MS.gene047519.t1	MTR_8g073140	56.977	86	10	1	30	115	1	59	2.66e-23	87.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene047519.t1	AT5G67510	88.356	146	17	0	1	146	1	146	9.99e-93	265
MS.gene047519.t1	AT3G49910	85.616	146	21	0	1	146	1	146	1.18e-91	262

Find 49 sgRNAs with CRISPR-Local

Find 47 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TATTATGCAGACCGTTGATT+AGG	0.363868	2.1:+72049513	MS.gene047519:CDS
CATTGAACGGACGTTGTACT+TGG	0.377101	2.1:-72049212	None:intergenic
TCGCAAAGACGACGAAGTTC+AGG	0.379749	2.1:+72049240	MS.gene047519:CDS
TTAGTGATAACAACCTTTGA+AGG	0.395167	2.1:-72049391	None:intergenic
ACTTCGTCGTCTTTGCGAAC+AGG	0.416424	2.1:-72049235	None:intergenic
GGGTCGTGCTGCTGCTGATA+AGG	0.420559	2.1:+72049462	MS.gene047519:CDS
ACGGCTCTTCCTACGGCTGC+TGG	0.422834	2.1:-72049116	None:intergenic
AAGACACGGCGGACGCTTGA+TGG	0.437229	2.1:-72049157	None:intergenic
CACTAAGCTCAGATTGGATA+AGG	0.438891	2.1:+72049408	MS.gene047519:CDS
AGGATTAACACCAACATTAA+CGG	0.444745	2.1:-72049371	None:intergenic
CCTGAGTAACCTTACCTTCA+CGG	0.446679	2.1:-72049285	None:intergenic
TGTTATCACTAAGCTCAGAT+TGG	0.466490	2.1:+72049402	MS.gene047519:CDS
GACGAAGTTCAGGTCGTTCG+TGG	0.488796	2.1:+72049250	MS.gene047519:CDS
ATTCAAAGGCCGTGAAGGTA+AGG	0.498862	2.1:+72049276	MS.gene047519:CDS
CTCAGGTTTACCGTCGCAAG+TGG	0.512762	2.1:+72049302	MS.gene047519:CDS
GCTTCTTGATCGTAAGGCTA+AGG	0.522703	2.1:+72049441	MS.gene047519:CDS
TGTTGGTGTTAATCCTTCAA+AGG	0.524932	2.1:+72049378	MS.gene047519:CDS
CTACGGCTGCTGGAAACTCG+TGG	0.530535	2.1:-72049106	None:intergenic
TGCTGCTGCTGATAAGGAGA+AGG	0.537077	2.1:+72049468	MS.gene047519:CDS
GAGCCGTAAGGCTCATTTCA+CGG	0.545137	2.1:+72049132	MS.gene047519:CDS
ATCACAAGGGAGAAGGTGAA+TGG	0.545267	2.1:+72049343	MS.gene047519:CDS
GAGCCTTACGGCTCTTCCTA+CGG	0.547173	2.1:-72049123	None:intergenic
TAAATCGCTTCTTGATCGTA+AGG	0.549460	2.1:+72049435	MS.gene047519:CDS
GTCGTTCGTGGAACATTCAA+AGG	0.554383	2.1:+72049262	MS.gene047519:CDS
CTTCTTGATCGTAAGGCTAA+GGG	0.554681	2.1:+72049442	MS.gene047519:CDS
GGGTACTAAGTTTGCTCCTG+AGG	0.563912	2.1:+72049489	MS.gene047519:CDS
AACGGACGTTGTACTTGGAT+CGG	0.564652	2.1:-72049207	None:intergenic
CCGTGAAGGTAAGGTTACTC+AGG	0.566699	2.1:+72049285	MS.gene047519:CDS
CTTTGCGAACAGGCATTGAA+CGG	0.570326	2.1:-72049225	None:intergenic
GTTGTACTTGGATCGGAGAT+CGG	0.572814	2.1:-72049200	None:intergenic
ACGGTCTGCATAATATCCTC+AGG	0.575139	2.1:-72049505	None:intergenic
GCGCCGTGAAATGAGCCTTA+CGG	0.575259	2.1:-72049135	None:intergenic
CGAACGCATCACAAGGGAGA+AGG	0.580140	2.1:+72049336	MS.gene047519:CDS
GATCGGAGATCGGCGGAGAG+TGG	0.584970	2.1:-72049190	None:intergenic
TACGGCTGCTGGAAACTCGT+GGG	0.586325	2.1:-72049105	None:intergenic
TTCACATCGAACGCATCACA+AGG	0.588472	2.1:+72049329	MS.gene047519:CDS
CAGCCGTAGGAAGAGCCGTA+AGG	0.591177	2.1:+72049120	MS.gene047519:CDS
GGAACATTCAAAGGCCGTGA+AGG	0.606062	2.1:+72049271	MS.gene047519:CDS
GATTCAAGAATCCTAATCAA+CGG	0.625900	2.1:-72049524	None:intergenic
AATGGATCCACCGTTAATGT+TGG	0.632764	2.1:+72049361	MS.gene047519:CDS
GCTGCTGCTGATAAGGAGAA+GGG	0.634378	2.1:+72049469	MS.gene047519:CDS
TGTGAATCACCCACTTGCGA+CGG	0.658219	2.1:-72049312	None:intergenic
ATTAACACCAACATTAACGG+TGG	0.662702	2.1:-72049368	None:intergenic
TCAGGTTTACCGTCGCAAGT+GGG	0.670860	2.1:+72049303	MS.gene047519:CDS
CACGAGTTTCCAGCAGCCGT+AGG	0.686122	2.1:+72049107	MS.gene047519:CDS
GTGGCGCGCTCATTAAGACA+CGG	0.705562	2.1:-72049171	None:intergenic
GTACTTGGATCGGAGATCGG+CGG	0.709980	2.1:-72049197	None:intergenic
TCACATCGAACGCATCACAA+GGG	0.710980	2.1:+72049330	MS.gene047519:CDS
GCGCGCTCATTAAGACACGG+CGG	0.823768	2.1:-72049168	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AGGATTAACACCAACATTAA+CGG	-	chr2.1:72049374-72049393	None:intergenic	30.0%
	TTAGTGATAACAACCTTTGA+AGG	-	chr2.1:72049394-72049413	None:intergenic	30.0%
	ATTAACACCAACATTAACGG+TGG	-	chr2.1:72049371-72049390	None:intergenic	35.0%
	TAAATCGCTTCTTGATCGTA+AGG	+	chr2.1:72049435-72049454	MS.gene047519:CDS	35.0%
	TGTTATCACTAAGCTCAGAT+TGG	+	chr2.1:72049402-72049421	MS.gene047519:CDS	35.0%
!!	TGTTGGTGTTAATCCTTCAA+AGG	+	chr2.1:72049378-72049397	MS.gene047519:CDS	35.0%
	AATGGATCCACCGTTAATGT+TGG	+	chr2.1:72049361-72049380	MS.gene047519:CDS	40.0%
	CACTAAGCTCAGATTGGATA+AGG	+	chr2.1:72049408-72049427	MS.gene047519:CDS	40.0%
	CTTCTTGATCGTAAGGCTAA+GGG	+	chr2.1:72049442-72049461	MS.gene047519:CDS	40.0%
	AACGGACGTTGTACTTGGAT+CGG	-	chr2.1:72049210-72049229	None:intergenic	45.0%
	ACGGTCTGCATAATATCCTC+AGG	-	chr2.1:72049508-72049527	None:intergenic	45.0%
	ATCACAAGGGAGAAGGTGAA+TGG	+	chr2.1:72049343-72049362	MS.gene047519:CDS	45.0%
	ATTCAAAGGCCGTGAAGGTA+AGG	+	chr2.1:72049276-72049295	MS.gene047519:CDS	45.0%
	CATTGAACGGACGTTGTACT+TGG	-	chr2.1:72049215-72049234	None:intergenic	45.0%
	CCTGAGTAACCTTACCTTCA+CGG	-	chr2.1:72049288-72049307	None:intergenic	45.0%
	GCTTCTTGATCGTAAGGCTA+AGG	+	chr2.1:72049441-72049460	MS.gene047519:CDS	45.0%
	GTCGTTCGTGGAACATTCAA+AGG	+	chr2.1:72049262-72049281	MS.gene047519:CDS	45.0%
	GTTGTACTTGGATCGGAGAT+CGG	-	chr2.1:72049203-72049222	None:intergenic	45.0%
	TCACATCGAACGCATCACAA+GGG	+	chr2.1:72049330-72049349	MS.gene047519:CDS	45.0%
	TTCACATCGAACGCATCACA+AGG	+	chr2.1:72049329-72049348	MS.gene047519:CDS	45.0%
!!	CTTTGCGAACAGGCATTGAA+CGG	-	chr2.1:72049228-72049247	None:intergenic	45.0%
	ACTTCGTCGTCTTTGCGAAC+AGG	-	chr2.1:72049238-72049257	None:intergenic	50.0%
	CCGTGAAGGTAAGGTTACTC+AGG	+	chr2.1:72049285-72049304	MS.gene047519:CDS	50.0%
	GAGCCGTAAGGCTCATTTCA+CGG	+	chr2.1:72049132-72049151	MS.gene047519:CDS	50.0%
	GCTGCTGCTGATAAGGAGAA+GGG	+	chr2.1:72049469-72049488	MS.gene047519:CDS	50.0%
	GGAACATTCAAAGGCCGTGA+AGG	+	chr2.1:72049271-72049290	MS.gene047519:CDS	50.0%
	TCAGGTTTACCGTCGCAAGT+GGG	+	chr2.1:72049303-72049322	MS.gene047519:CDS	50.0%
	TCGCAAAGACGACGAAGTTC+AGG	+	chr2.1:72049240-72049259	MS.gene047519:CDS	50.0%
	TGCTGCTGCTGATAAGGAGA+AGG	+	chr2.1:72049468-72049487	MS.gene047519:CDS	50.0%
	TGTGAATCACCCACTTGCGA+CGG	-	chr2.1:72049315-72049334	None:intergenic	50.0%
!	GGGTACTAAGTTTGCTCCTG+AGG	+	chr2.1:72049489-72049508	MS.gene047519:CDS	50.0%
	CGAACGCATCACAAGGGAGA+AGG	+	chr2.1:72049336-72049355	MS.gene047519:CDS	55.0%
	CTCAGGTTTACCGTCGCAAG+TGG	+	chr2.1:72049302-72049321	MS.gene047519:CDS	55.0%
	GACGAAGTTCAGGTCGTTCG+TGG	+	chr2.1:72049250-72049269	MS.gene047519:CDS	55.0%
	GAGCCTTACGGCTCTTCCTA+CGG	-	chr2.1:72049126-72049145	None:intergenic	55.0%
	GCGCCGTGAAATGAGCCTTA+CGG	-	chr2.1:72049138-72049157	None:intergenic	55.0%
	GTACTTGGATCGGAGATCGG+CGG	-	chr2.1:72049200-72049219	None:intergenic	55.0%
	GTGGCGCGCTCATTAAGACA+CGG	-	chr2.1:72049174-72049193	None:intergenic	55.0%
	TACGGCTGCTGGAAACTCGT+GGG	-	chr2.1:72049108-72049127	None:intergenic	55.0%
	AAGACACGGCGGACGCTTGA+TGG	-	chr2.1:72049160-72049179	None:intergenic	60.0%
	CACGAGTTTCCAGCAGCCGT+AGG	+	chr2.1:72049107-72049126	MS.gene047519:CDS	60.0%
	CAGCCGTAGGAAGAGCCGTA+AGG	+	chr2.1:72049120-72049139	MS.gene047519:CDS	60.0%
	CTACGGCTGCTGGAAACTCG+TGG	-	chr2.1:72049109-72049128	None:intergenic	60.0%
	GCGCGCTCATTAAGACACGG+CGG	-	chr2.1:72049171-72049190	None:intergenic	60.0%
!	GGGTCGTGCTGCTGCTGATA+AGG	+	chr2.1:72049462-72049481	MS.gene047519:CDS	60.0%
	ACGGCTCTTCCTACGGCTGC+TGG	-	chr2.1:72049119-72049138	None:intergenic	65.0%
	GATCGGAGATCGGCGGAGAG+TGG	-	chr2.1:72049193-72049212	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.1	gene	72049094	72049534	72049094	ID=MS.gene047519
chr2.1	mRNA	72049094	72049534	72049094	ID=MS.gene047519.t1;Parent=MS.gene047519
chr2.1	exon	72049094	72049534	72049094	ID=MS.gene047519.t1.exon1;Parent=MS.gene047519.t1
chr2.1	CDS	72049094	72049534	72049094	ID=cds.MS.gene047519.t1;Parent=MS.gene047519.t1

Gene Sequence

>MS.gene047519

ATGAAGTTCAACCCACGAGTTTCCAGCAGCCGTAGGAAGAGCCGTAAGGCTCATTTCACGGCGCCATCAAGCGTCCGCCGTGTCTTAATGAGCGCGCCACTCTCCGCCGATCTCCGATCCAAGTACAACGTCCGTTCAATGCCTGTTCGCAAAGACGACGAAGTTCAGGTCGTTCGTGGAACATTCAAAGGCCGTGAAGGTAAGGTTACTCAGGTTTACCGTCGCAAGTGGGTGATTCACATCGAACGCATCACAAGGGAGAAGGTGAATGGATCCACCGTTAATGTTGGTGTTAATCCTTCAAAGGTTGTTATCACTAAGCTCAGATTGGATAAGGATCGTAAATCGCTTCTTGATCGTAAGGCTAAGGGTCGTGCTGCTGCTGATAAGGAGAAGGGTACTAAGTTTGCTCCTGAGGATATTATGCAGACCGTTGATTAG

Protein sequence

>MS.gene047519.t1

MKFNPRVSSSRRKSRKAHFTAPSSVRRVLMSAPLSADLRSKYNVRSMPVRKDDEVQVVRGTFKGREGKVTQVYRRKWVIHIERITREKVNGSTVNVGVNPSKVVITKLRLDKDRKSLLDRKAKGRAAADKEKGTKFAPEDIMQTVD