AlfalfaGEDB | MS.gene09597

M. sativa cultivar XinJiangDaYe / MS.gene09597

KEGG: K02898
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0015934 GO:0019538 GO:0032991 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044391 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576 GO:1990904
PFAM: PF00467.32 PF16906.8

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene09597.t1	XP_003611586.2	100	146	0	0	1	146	1	146	3.60E-72	280.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene09597.t1	P51414	85.6	146	21	0	1	146	1	146	1.3e-65	250.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene09597.t1	G7K2J3	100.0	146	0	0	1	146	1	146	2.6e-72	280.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene057641	MS.gene09597	0.803312	3.70E-49	-1.69E-46
MS.gene059840	MS.gene09597	0.809734	1.63E-50	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene015255	MS.gene09597	PPI
MS.gene01360	MS.gene09597	PPI
MS.gene43544	MS.gene09597	PPI
MS.gene20295	MS.gene09597	PPI
MS.gene002382	MS.gene09597	PPI
MS.gene047519	MS.gene09597	PPI
MS.gene01768	MS.gene09597	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene09597.t1	MTR_5g015570	100.000	146	0	0	1	146	1	146	3.19e-103	291
MS.gene09597.t1	MTR_2g012450	95.205	146	7	0	1	146	1	146	2.71e-90	259
MS.gene09597.t1	MTR_5g096540	91.781	146	12	0	1	146	1	146	8.14e-88	253
MS.gene09597.t1	MTR_4g126220	92.647	68	5	0	13	80	103	170	1.42e-39	131
MS.gene09597.t1	MTR_8g073140	60.465	86	7	1	30	115	1	59	8.93e-25	90.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene09597.t1	AT5G67510	86.301	146	20	0	1	146	1	146	2.43e-91	261
MS.gene09597.t1	AT3G49910	85.616	146	21	0	1	146	1	146	2.43e-91	261

Find 47 sgRNAs with CRISPR-Local

Find 46 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTTCCCTTCACGTCCCTTGA+AGG	0.334674	5.2:-7358860	None:intergenic
TGCAAGTTTATCGTAGGAAA+TGG	0.354784	5.2:+7358887	MS.gene09597:CDS
TTAGTAATCACAACCTTTGA+AGG	0.371196	5.2:-7358976	None:intergenic
TACTAAGATTCGTTTGGATA+AGG	0.384251	5.2:+7358993	MS.gene09597:CDS
TGTGATTACTAAGATTCGTT+TGG	0.392723	5.2:+7358987	MS.gene09597:CDS
AAAACACGACGGACGCTTGA+TGG	0.398070	5.2:-7358742	None:intergenic
ATTACACGTGAGAAGGTTAA+TGG	0.405029	5.2:+7358928	MS.gene09597:CDS
GGGTCGTGCTGCTGCTGATA+AGG	0.420559	5.2:+7359047	MS.gene09597:CDS
TCGTAAGGATGATGAAGTTC+AGG	0.424559	5.2:+7358825	MS.gene09597:CDS
CGACGGCTGCTTGAAACCCT+AGG	0.429571	5.2:-7358691	None:intergenic
TGCTGCTGCTGATAAGGAAA+AGG	0.437551	5.2:+7359053	MS.gene09597:CDS
AAACACGACGGACGCTTGAT+GGG	0.465655	5.2:-7358741	None:intergenic
AATGTCTTCAGGAGCAAACT+TGG	0.473256	5.2:-7359079	None:intergenic
GCGTTCTCTTCCCGTTCGTA+AGG	0.475960	5.2:+7358810	MS.gene09597:CDS
GAAAAGCTTGCTTGATCGTA+AGG	0.501897	5.2:+7359020	MS.gene09597:CDS
GCAAGTTTATCGTAGGAAAT+GGG	0.504322	5.2:+7358888	MS.gene09597:CDS
ACTTCATCATCCTTACGAAC+GGG	0.506464	5.2:-7358820	None:intergenic
TTGATGAGCGCACCTCTCTC+CGG	0.508962	5.2:+7358763	MS.gene09597:CDS
ACACAATGAAGTTCAACCCT+AGG	0.518183	5.2:+7358674	None:intergenic
GGTAGTGAGAGGAACCTTCA+AGG	0.522192	5.2:+7358846	MS.gene09597:CDS
GTACTTTGAACGGAGGTCAC+CGG	0.530458	5.2:-7358782	None:intergenic
CAGCCGTCGCAAGAGCCGCA+AGG	0.548428	5.2:+7358705	MS.gene09597:CDS
CACAATGAAGTTCAACCCTA+GGG	0.551275	5.2:+7358675	None:intergenic
AATGGGTGATTCATATTGAG+AGG	0.559538	5.2:+7358905	MS.gene09597:CDS
AACTTCATCATCCTTACGAA+CGG	0.563073	5.2:-7358821	None:intergenic
GGAACCTTCAAGGGACGTGA+AGG	0.563323	5.2:+7358856	MS.gene09597:CDS
ACGACGGACGCTTGATGGGG+CGG	0.563774	5.2:-7358737	None:intergenic
GCTGCTGCTGATAAGGAAAA+GGG	0.564379	5.2:+7359054	MS.gene09597:CDS
GACGGCTGCTTGAAACCCTA+GGG	0.565492	5.2:-7358690	None:intergenic
TGTTGGTGTTGATCCTTCAA+AGG	0.568879	5.2:+7358963	MS.gene09597:CDS
GAGCCTTGCGGCTCTTGCGA+CGG	0.572981	5.2:-7358708	None:intergenic
CTTGCTTGATCGTAAGGCGA+AGG	0.575754	5.2:+7359026	MS.gene09597:CDS
AACGCACGTTGTACTTTGAA+CGG	0.578356	5.2:-7358792	None:intergenic
AGATTCGTTTGGATAAGGAT+AGG	0.578817	5.2:+7358998	MS.gene09597:CDS
AACACGACGGACGCTTGATG+GGG	0.578903	5.2:-7358740	None:intergenic
TGAGAGGATTACACGTGAGA+AGG	0.580146	5.2:+7358921	MS.gene09597:CDS
GATGAAGTTCAGGTAGTGAG+AGG	0.591688	5.2:+7358835	MS.gene09597:CDS
CTTCAAGGGACGTGAAGGGA+AGG	0.593141	5.2:+7358861	MS.gene09597:CDS
AGGTTGTGCAAGTTTATCGT+AGG	0.600876	5.2:+7358881	MS.gene09597:CDS
AATGGTCAAACTGTGAATGT+TGG	0.612366	5.2:+7358946	MS.gene09597:CDS
TTGCTTGATCGTAAGGCGAA+GGG	0.621055	5.2:+7359027	MS.gene09597:CDS
GAACGGAGGTCACCGGAGAG+AGG	0.625041	5.2:-7358775	None:intergenic
GTAGTGAGAGGAACCTTCAA+GGG	0.634832	5.2:+7358847	MS.gene09597:CDS
GAACCTTCAAGGGACGTGAA+GGG	0.643186	5.2:+7358857	MS.gene09597:CDS
GCACGTTGTACTTTGAACGG+AGG	0.673729	5.2:-7358789	None:intergenic
GGGCGGTGAAATGAGCCTTG+CGG	0.681827	5.2:-7358720	None:intergenic
GTGCGCTCATCAAAACACGA+CGG	0.772319	5.2:-7358753	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TACTAAGATTCGTTTGGATA+AGG	+	chr5.2:7358993-7359012	MS.gene09597:CDS	30.0%
	TGTGATTACTAAGATTCGTT+TGG	+	chr5.2:7358987-7359006	MS.gene09597:CDS	30.0%
	TTAGTAATCACAACCTTTGA+AGG	-	chr5.2:7358979-7358998	None:intergenic	30.0%
	AACTTCATCATCCTTACGAA+CGG	-	chr5.2:7358824-7358843	None:intergenic	35.0%
	AATGGGTGATTCATATTGAG+AGG	+	chr5.2:7358905-7358924	MS.gene09597:CDS	35.0%
	AATGGTCAAACTGTGAATGT+TGG	+	chr5.2:7358946-7358965	MS.gene09597:CDS	35.0%
	AGATTCGTTTGGATAAGGAT+AGG	+	chr5.2:7358998-7359017	MS.gene09597:CDS	35.0%
	ATTACACGTGAGAAGGTTAA+TGG	+	chr5.2:7358928-7358947	MS.gene09597:CDS	35.0%
	GCAAGTTTATCGTAGGAAAT+GGG	+	chr5.2:7358888-7358907	MS.gene09597:CDS	35.0%
	TGCAAGTTTATCGTAGGAAA+TGG	+	chr5.2:7358887-7358906	MS.gene09597:CDS	35.0%
!	ACGTTTTGCATAATGTCTTC+AGG	-	chr5.2:7359093-7359112	None:intergenic	35.0%
	AACGCACGTTGTACTTTGAA+CGG	-	chr5.2:7358795-7358814	None:intergenic	40.0%
	AATGTCTTCAGGAGCAAACT+TGG	-	chr5.2:7359082-7359101	None:intergenic	40.0%
	ACTTCATCATCCTTACGAAC+GGG	-	chr5.2:7358823-7358842	None:intergenic	40.0%
	AGGTTGTGCAAGTTTATCGT+AGG	+	chr5.2:7358881-7358900	MS.gene09597:CDS	40.0%
	TCGTAAGGATGATGAAGTTC+AGG	+	chr5.2:7358825-7358844	MS.gene09597:CDS	40.0%
!	GAAAAGCTTGCTTGATCGTA+AGG	+	chr5.2:7359020-7359039	MS.gene09597:CDS	40.0%
!!	TGTTGGTGTTGATCCTTCAA+AGG	+	chr5.2:7358963-7358982	MS.gene09597:CDS	40.0%
	GATGAAGTTCAGGTAGTGAG+AGG	+	chr5.2:7358835-7358854	MS.gene09597:CDS	45.0%
	GCTGCTGCTGATAAGGAAAA+GGG	+	chr5.2:7359054-7359073	MS.gene09597:CDS	45.0%
	GTAGTGAGAGGAACCTTCAA+GGG	+	chr5.2:7358847-7358866	MS.gene09597:CDS	45.0%
	TGAGAGGATTACACGTGAGA+AGG	+	chr5.2:7358921-7358940	MS.gene09597:CDS	45.0%
	TGCTGCTGCTGATAAGGAAA+AGG	+	chr5.2:7359053-7359072	MS.gene09597:CDS	45.0%
	TTGCTTGATCGTAAGGCGAA+GGG	+	chr5.2:7359027-7359046	MS.gene09597:CDS	45.0%
	AAAACACGACGGACGCTTGA+TGG	-	chr5.2:7358745-7358764	None:intergenic	50.0%
	AAACACGACGGACGCTTGAT+GGG	-	chr5.2:7358744-7358763	None:intergenic	50.0%
	CTTGCTTGATCGTAAGGCGA+AGG	+	chr5.2:7359026-7359045	MS.gene09597:CDS	50.0%
	GAACCTTCAAGGGACGTGAA+GGG	+	chr5.2:7358857-7358876	MS.gene09597:CDS	50.0%
	GCACGTTGTACTTTGAACGG+AGG	-	chr5.2:7358792-7358811	None:intergenic	50.0%
	GGTAGTGAGAGGAACCTTCA+AGG	+	chr5.2:7358846-7358865	MS.gene09597:CDS	50.0%
	GTACTTTGAACGGAGGTCAC+CGG	-	chr5.2:7358785-7358804	None:intergenic	50.0%
	GTGCGCTCATCAAAACACGA+CGG	-	chr5.2:7358756-7358775	None:intergenic	50.0%
	AACACGACGGACGCTTGATG+GGG	-	chr5.2:7358743-7358762	None:intergenic	55.0%
	CTTCAAGGGACGTGAAGGGA+AGG	+	chr5.2:7358861-7358880	MS.gene09597:CDS	55.0%
	CTTCCCTTCACGTCCCTTGA+AGG	-	chr5.2:7358863-7358882	None:intergenic	55.0%
	GACGGCTGCTTGAAACCCTA+GGG	-	chr5.2:7358693-7358712	None:intergenic	55.0%
	GCGTTCTCTTCCCGTTCGTA+AGG	+	chr5.2:7358810-7358829	MS.gene09597:CDS	55.0%
	GGAACCTTCAAGGGACGTGA+AGG	+	chr5.2:7358856-7358875	MS.gene09597:CDS	55.0%
	TTGATGAGCGCACCTCTCTC+CGG	+	chr5.2:7358763-7358782	MS.gene09597:CDS	55.0%
	CGACGGCTGCTTGAAACCCT+AGG	-	chr5.2:7358694-7358713	None:intergenic	60.0%
	GGGCGGTGAAATGAGCCTTG+CGG	-	chr5.2:7358723-7358742	None:intergenic	60.0%
!	GGGTCGTGCTGCTGCTGATA+AGG	+	chr5.2:7359047-7359066	MS.gene09597:CDS	60.0%
	ACGACGGACGCTTGATGGGG+CGG	-	chr5.2:7358740-7358759	None:intergenic	65.0%
	GAACGGAGGTCACCGGAGAG+AGG	-	chr5.2:7358778-7358797	None:intergenic	65.0%
	GAGCCTTGCGGCTCTTGCGA+CGG	-	chr5.2:7358711-7358730	None:intergenic	65.0%
	CAGCCGTCGCAAGAGCCGCA+AGG	+	chr5.2:7358705-7358724	MS.gene09597:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.2	gene	7358679	7359119	7358679	ID=MS.gene09597
chr5.2	mRNA	7358679	7359119	7358679	ID=MS.gene09597.t1;Parent=MS.gene09597
chr5.2	exon	7358679	7359119	7358679	ID=MS.gene09597.t1.exon1;Parent=MS.gene09597.t1
chr5.2	CDS	7358679	7359119	7358679	ID=cds.MS.gene09597.t1;Parent=MS.gene09597.t1

Gene Sequence

>MS.gene09597

ATGAAGTTCAACCCTAGGGTTTCAAGCAGCCGTCGCAAGAGCCGCAAGGCTCATTTCACCGCCCCATCAAGCGTCCGTCGTGTTTTGATGAGCGCACCTCTCTCCGGTGACCTCCGTTCAAAGTACAACGTGCGTTCTCTTCCCGTTCGTAAGGATGATGAAGTTCAGGTAGTGAGAGGAACCTTCAAGGGACGTGAAGGGAAGGTTGTGCAAGTTTATCGTAGGAAATGGGTGATTCATATTGAGAGGATTACACGTGAGAAGGTTAATGGTCAAACTGTGAATGTTGGTGTTGATCCTTCAAAGGTTGTGATTACTAAGATTCGTTTGGATAAGGATAGGAAAAGCTTGCTTGATCGTAAGGCGAAGGGTCGTGCTGCTGCTGATAAGGAAAAGGGCACCAAGTTTGCTCCTGAAGACATTATGCAAAACGTTGATTAG

Protein sequence

>MS.gene09597.t1

MKFNPRVSSSRRKSRKAHFTAPSSVRRVLMSAPLSGDLRSKYNVRSLPVRKDDEVQVVRGTFKGREGKVVQVYRRKWVIHIERITREKVNGQTVNVGVDPSKVVITKIRLDKDRKSLLDRKAKGRAAADKEKGTKFAPEDIMQNVD