AlfalfaGEDB | MS.gene015255

M. sativa cultivar XinJiangDaYe / MS.gene015255

KEGG: K02898
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0015934 GO:0019538 GO:0032991 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044391 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576 GO:1990904
PFAM: PF00467.32 PF16906.8

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene015255.t1	XP_003611586.2	99.3	146	1	0	1	146	1	146	1.10E-71	279.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene015255.t1	P51414	85.6	146	21	0	1	146	1	146	2.2e-65	249.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene015255.t1	G7K2J3	99.3	146	1	0	1	146	1	146	7.6e-72	279.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene015255	MS.gene09597	PPI
MS.gene047519	MS.gene015255	PPI
MS.gene01768	MS.gene015255	PPI
MS.gene015255	MS.gene43544	PPI
MS.gene01360	MS.gene015255	PPI
MS.gene015255	MS.gene002382	PPI
MS.gene43544	MS.gene015255	PPI
MS.gene20295	MS.gene015255	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene015255.t1	MTR_5g015570	99.315	146	1	0	1	146	1	146	1.33e-102	290
MS.gene015255.t1	MTR_2g012450	94.521	146	8	0	1	146	1	146	1.29e-89	257
MS.gene015255.t1	MTR_5g096540	91.781	146	12	0	1	146	1	146	2.49e-87	251
MS.gene015255.t1	MTR_4g126220	91.176	68	6	0	13	80	103	170	7.75e-39	129
MS.gene015255.t1	MTR_8g073140	60.465	86	7	1	30	115	1	59	8.46e-25	90.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene015255.t1	AT3G49910	85.616	146	21	0	1	146	1	146	4.35e-91	261
MS.gene015255.t1	AT5G67510	85.616	146	21	0	1	146	1	146	9.28e-91	260

Find 47 sgRNAs with CRISPR-Local

Find 46 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTTCCCTTCACGTCCCTTGA+AGG	0.334674	5.4:-9139970	None:intergenic
TGCAAGTTTATCGTAGGAAA+TGG	0.354784	5.4:+9139997	MS.gene015255:CDS
TACCAAGATTCGTTTGGATA+AGG	0.385625	5.4:+9140103	MS.gene015255:CDS
ATCCTTATCCAAACGAATCT+TGG	0.396970	5.4:-9140105	None:intergenic
ATTACACGTGAGAAGGTTAA+TGG	0.405029	5.4:+9140038	MS.gene015255:CDS
TGTGATTACCAAGATTCGTT+TGG	0.408401	5.4:+9140097	MS.gene015255:CDS
TTGGTAATCACAACCTTTGA+AGG	0.416639	5.4:-9140086	None:intergenic
GGGTCGTGCTGCTGCTGATA+AGG	0.420559	5.4:+9140157	MS.gene015255:CDS
AAAACACGACGGACGCTTGA+TGG	0.423167	5.4:-9139852	None:intergenic
TCGTAAGGATGATGAAGTTC+AGG	0.424559	5.4:+9139935	MS.gene015255:CDS
CGACGGCTGCTTGAAACCCT+AGG	0.429571	5.4:-9139801	None:intergenic
GCGTTCTCTTCCCGTTCGTA+AGG	0.475960	5.4:+9139920	MS.gene015255:CDS
TTCATGAGCGCACCTCTCTC+CGG	0.482972	5.4:+9139873	MS.gene015255:CDS
GAAAAGCTTGCTTGATCGTA+AGG	0.501897	5.4:+9140130	MS.gene015255:CDS
GCAAGTTTATCGTAGGAAAT+GGG	0.504322	5.4:+9139998	MS.gene015255:CDS
ACTTCATCATCCTTACGAAC+GGG	0.506464	5.4:-9139930	None:intergenic
AAACACGACGGACGCTTGAT+GGG	0.506764	5.4:-9139851	None:intergenic
ACACAATGAAGTTCAACCCT+AGG	0.518183	5.4:+9139784	None:intergenic
GGTAGTGAGAGGAACCTTCA+AGG	0.522192	5.4:+9139956	MS.gene015255:CDS
GTACTTTGAACGGAGGTCAC+CGG	0.530458	5.4:-9139892	None:intergenic
TGCTGCTGCTGATAAGGAGA+AGG	0.537077	5.4:+9140163	MS.gene015255:CDS
CAGCCGTCGCAAGAGCCGCA+AGG	0.548428	5.4:+9139815	MS.gene015255:CDS
CACAATGAAGTTCAACCCTA+GGG	0.551275	5.4:+9139785	None:intergenic
AATGGGTGATTCATATTGAG+AGG	0.559538	5.4:+9140015	MS.gene015255:CDS
AACTTCATCATCCTTACGAA+CGG	0.563073	5.4:-9139931	None:intergenic
GGAACCTTCAAGGGACGTGA+AGG	0.563323	5.4:+9139966	MS.gene015255:CDS
ACGACGGACGCTTGATGGGG+CGG	0.563774	5.4:-9139847	None:intergenic
GACGGCTGCTTGAAACCCTA+GGG	0.565492	5.4:-9139800	None:intergenic
TGTTGGTGTTGATCCTTCAA+AGG	0.568879	5.4:+9140073	MS.gene015255:CDS
GAGCCTTGCGGCTCTTGCGA+CGG	0.572981	5.4:-9139818	None:intergenic
CTTGCTTGATCGTAAGGCGA+AGG	0.575754	5.4:+9140136	MS.gene015255:CDS
AACACGACGGACGCTTGATG+GGG	0.576464	5.4:-9139850	None:intergenic
AACGCACGTTGTACTTTGAA+CGG	0.578356	5.4:-9139902	None:intergenic
AGATTCGTTTGGATAAGGAT+AGG	0.578817	5.4:+9140108	MS.gene015255:CDS
TGAGAGGATTACACGTGAGA+AGG	0.580146	5.4:+9140031	MS.gene015255:CDS
GATGAAGTTCAGGTAGTGAG+AGG	0.591688	5.4:+9139945	MS.gene015255:CDS
CTTCAAGGGACGTGAAGGGA+AGG	0.593141	5.4:+9139971	MS.gene015255:CDS
AGGTTGTGCAAGTTTATCGT+AGG	0.600876	5.4:+9139991	MS.gene015255:CDS
AATGGTCAAACTGTGAATGT+TGG	0.612366	5.4:+9140056	MS.gene015255:CDS
TTGCTTGATCGTAAGGCGAA+GGG	0.621055	5.4:+9140137	MS.gene015255:CDS
GAACGGAGGTCACCGGAGAG+AGG	0.625041	5.4:-9139885	None:intergenic
GCTGCTGCTGATAAGGAGAA+GGG	0.634378	5.4:+9140164	MS.gene015255:CDS
GTAGTGAGAGGAACCTTCAA+GGG	0.634832	5.4:+9139957	MS.gene015255:CDS
GAACCTTCAAGGGACGTGAA+GGG	0.643186	5.4:+9139967	MS.gene015255:CDS
GCACGTTGTACTTTGAACGG+AGG	0.673729	5.4:-9139899	None:intergenic
GGGCGGTGAAATGAGCCTTG+CGG	0.681827	5.4:-9139830	None:intergenic
GTGCGCTCATGAAAACACGA+CGG	0.778073	5.4:-9139863	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AACTTCATCATCCTTACGAA+CGG	-	chr5.4:9139934-9139953	None:intergenic	35.0%
	AATGGGTGATTCATATTGAG+AGG	+	chr5.4:9140015-9140034	MS.gene015255:CDS	35.0%
	AATGGTCAAACTGTGAATGT+TGG	+	chr5.4:9140056-9140075	MS.gene015255:CDS	35.0%
	AGATTCGTTTGGATAAGGAT+AGG	+	chr5.4:9140108-9140127	MS.gene015255:CDS	35.0%
	ATCCTTATCCAAACGAATCT+TGG	-	chr5.4:9140108-9140127	None:intergenic	35.0%
	ATTACACGTGAGAAGGTTAA+TGG	+	chr5.4:9140038-9140057	MS.gene015255:CDS	35.0%
	GCAAGTTTATCGTAGGAAAT+GGG	+	chr5.4:9139998-9140017	MS.gene015255:CDS	35.0%
	TACCAAGATTCGTTTGGATA+AGG	+	chr5.4:9140103-9140122	MS.gene015255:CDS	35.0%
	TGCAAGTTTATCGTAGGAAA+TGG	+	chr5.4:9139997-9140016	MS.gene015255:CDS	35.0%
	TGTGATTACCAAGATTCGTT+TGG	+	chr5.4:9140097-9140116	MS.gene015255:CDS	35.0%
!	ACGTTTTGCATAATGTCTTC+AGG	-	chr5.4:9140203-9140222	None:intergenic	35.0%
!	TTGGTAATCACAACCTTTGA+AGG	-	chr5.4:9140089-9140108	None:intergenic	35.0%
	AACGCACGTTGTACTTTGAA+CGG	-	chr5.4:9139905-9139924	None:intergenic	40.0%
	ACTTCATCATCCTTACGAAC+GGG	-	chr5.4:9139933-9139952	None:intergenic	40.0%
	AGGTTGTGCAAGTTTATCGT+AGG	+	chr5.4:9139991-9140010	MS.gene015255:CDS	40.0%
	TCGTAAGGATGATGAAGTTC+AGG	+	chr5.4:9139935-9139954	MS.gene015255:CDS	40.0%
!	GAAAAGCTTGCTTGATCGTA+AGG	+	chr5.4:9140130-9140149	MS.gene015255:CDS	40.0%
!!	TGTTGGTGTTGATCCTTCAA+AGG	+	chr5.4:9140073-9140092	MS.gene015255:CDS	40.0%
	GATGAAGTTCAGGTAGTGAG+AGG	+	chr5.4:9139945-9139964	MS.gene015255:CDS	45.0%
	GTAGTGAGAGGAACCTTCAA+GGG	+	chr5.4:9139957-9139976	MS.gene015255:CDS	45.0%
	TGAGAGGATTACACGTGAGA+AGG	+	chr5.4:9140031-9140050	MS.gene015255:CDS	45.0%
	TTGCTTGATCGTAAGGCGAA+GGG	+	chr5.4:9140137-9140156	MS.gene015255:CDS	45.0%
	AAAACACGACGGACGCTTGA+TGG	-	chr5.4:9139855-9139874	None:intergenic	50.0%
	AAACACGACGGACGCTTGAT+GGG	-	chr5.4:9139854-9139873	None:intergenic	50.0%
	CTTGCTTGATCGTAAGGCGA+AGG	+	chr5.4:9140136-9140155	MS.gene015255:CDS	50.0%
	GAACCTTCAAGGGACGTGAA+GGG	+	chr5.4:9139967-9139986	MS.gene015255:CDS	50.0%
	GCACGTTGTACTTTGAACGG+AGG	-	chr5.4:9139902-9139921	None:intergenic	50.0%
	GCTGCTGCTGATAAGGAGAA+GGG	+	chr5.4:9140164-9140183	MS.gene015255:CDS	50.0%
	GGTAGTGAGAGGAACCTTCA+AGG	+	chr5.4:9139956-9139975	MS.gene015255:CDS	50.0%
	GTACTTTGAACGGAGGTCAC+CGG	-	chr5.4:9139895-9139914	None:intergenic	50.0%
	GTGCGCTCATGAAAACACGA+CGG	-	chr5.4:9139866-9139885	None:intergenic	50.0%
	TGCTGCTGCTGATAAGGAGA+AGG	+	chr5.4:9140163-9140182	MS.gene015255:CDS	50.0%
	AACACGACGGACGCTTGATG+GGG	-	chr5.4:9139853-9139872	None:intergenic	55.0%
	CTTCAAGGGACGTGAAGGGA+AGG	+	chr5.4:9139971-9139990	MS.gene015255:CDS	55.0%
	CTTCCCTTCACGTCCCTTGA+AGG	-	chr5.4:9139973-9139992	None:intergenic	55.0%
	GACGGCTGCTTGAAACCCTA+GGG	-	chr5.4:9139803-9139822	None:intergenic	55.0%
	GCGTTCTCTTCCCGTTCGTA+AGG	+	chr5.4:9139920-9139939	MS.gene015255:CDS	55.0%
	GGAACCTTCAAGGGACGTGA+AGG	+	chr5.4:9139966-9139985	MS.gene015255:CDS	55.0%
	TTCATGAGCGCACCTCTCTC+CGG	+	chr5.4:9139873-9139892	MS.gene015255:CDS	55.0%
	CGACGGCTGCTTGAAACCCT+AGG	-	chr5.4:9139804-9139823	None:intergenic	60.0%
	GGGCGGTGAAATGAGCCTTG+CGG	-	chr5.4:9139833-9139852	None:intergenic	60.0%
!	GGGTCGTGCTGCTGCTGATA+AGG	+	chr5.4:9140157-9140176	MS.gene015255:CDS	60.0%
	ACGACGGACGCTTGATGGGG+CGG	-	chr5.4:9139850-9139869	None:intergenic	65.0%
	GAACGGAGGTCACCGGAGAG+AGG	-	chr5.4:9139888-9139907	None:intergenic	65.0%
	GAGCCTTGCGGCTCTTGCGA+CGG	-	chr5.4:9139821-9139840	None:intergenic	65.0%
	CAGCCGTCGCAAGAGCCGCA+AGG	+	chr5.4:9139815-9139834	MS.gene015255:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.4	gene	9139789	9140229	9139789	ID=MS.gene015255
chr5.4	mRNA	9139789	9140229	9139789	ID=MS.gene015255.t1;Parent=MS.gene015255
chr5.4	exon	9139789	9140229	9139789	ID=MS.gene015255.t1.exon1;Parent=MS.gene015255.t1
chr5.4	CDS	9139789	9140229	9139789	ID=cds.MS.gene015255.t1;Parent=MS.gene015255.t1

Gene Sequence

>MS.gene015255

ATGAAGTTCAACCCTAGGGTTTCAAGCAGCCGTCGCAAGAGCCGCAAGGCTCATTTCACCGCCCCATCAAGCGTCCGTCGTGTTTTCATGAGCGCACCTCTCTCCGGTGACCTCCGTTCAAAGTACAACGTGCGTTCTCTTCCCGTTCGTAAGGATGATGAAGTTCAGGTAGTGAGAGGAACCTTCAAGGGACGTGAAGGGAAGGTTGTGCAAGTTTATCGTAGGAAATGGGTGATTCATATTGAGAGGATTACACGTGAGAAGGTTAATGGTCAAACTGTGAATGTTGGTGTTGATCCTTCAAAGGTTGTGATTACCAAGATTCGTTTGGATAAGGATAGGAAAAGCTTGCTTGATCGTAAGGCGAAGGGTCGTGCTGCTGCTGATAAGGAGAAGGGTACTAAGTTTGCTCCTGAAGACATTATGCAAAACGTTGATTAG

Protein sequence

>MS.gene015255.t1

MKFNPRVSSSRRKSRKAHFTAPSSVRRVFMSAPLSGDLRSKYNVRSLPVRKDDEVQVVRGTFKGREGKVVQVYRRKWVIHIERITREKVNGQTVNVGVDPSKVVITKIRLDKDRKSLLDRKAKGRAAADKEKGTKFAPEDIMQNVD