AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene00618

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene00618.t1 MTR_2g006170 100.000 135 0 0 1 135 1 135 4.36e-95 270
MS.gene00618.t1 MTR_2g035930 99.259 135 1 0 1 135 1 135 1.94e-94 268
MS.gene00618.t1 MTR_3g048580 64.179 67 15 2 70 135 38 96 2.91e-19 77.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene00618.t1 AT4G15000 85.926 135 19 0 1 135 1 135 2.61e-83 240
MS.gene00618.t1 AT3G22230 85.926 135 19 0 1 135 1 135 3.75e-83 240
MS.gene00618.t1 AT2G32220 80.741 135 26 0 1 135 1 135 1.73e-78 228
MS.gene00618.t1 AT4G15000 84.553 123 19 0 1 123 1 123 1.40e-72 213

Find 34 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTTTCTTCGAACCTCTTCT+TGG 0.194010 2.1:-75789187 None:intergenic
TACGGTCATTGCTTGGTTGC+AGG 0.251311 2.1:+75788961 MS.gene00618:CDS
CACTCTGGATGTTGATTTGA+AGG 0.342388 2.1:+75789104 MS.gene00618:CDS
TTCGAAGAAAGGTTCAAAAC+TGG 0.371776 2.1:+75789198 MS.gene00618:CDS
AGGACGTTACGCTGGCAAAA+AGG 0.377725 2.1:+75788894 MS.gene00618:CDS
AAGACGATCACCGCTTTGTT+TGG 0.378594 2.1:-75788868 None:intergenic
TCCTTCTTGATCACCTTAGC+TGG 0.435443 2.1:-75788997 None:intergenic
GGTTCAAAACTGGGAAGAAC+AGG 0.438118 2.1:+75789208 MS.gene00618:CDS
TAAGAAGGTCACTGCTTTGA+AGG 0.444100 2.1:+75789161 MS.gene00618:CDS
TGCAAAGAAATCGCGTGTTA+AGG 0.450525 2.1:+75789035 MS.gene00618:CDS
ACGGTCATTGCTTGGTTGCA+GGG 0.480537 2.1:+75788962 MS.gene00618:CDS
CCAAGAAGAGGTTCGAAGAA+AGG 0.486021 2.1:+75789187 MS.gene00618:CDS
GGAACCCGTGAACGTGCTTA+CGG 0.489757 2.1:+75788943 MS.gene00618:CDS
GGTGGTTCTTCACCAAGCTC+AGG 0.503030 2.1:+75789229 MS.gene00618:CDS
AATGACCGTAAGCACGTTCA+CGG 0.519577 2.1:-75788948 None:intergenic
CACTGATGTTCTTCAGAGCA+AGG 0.541833 2.1:+75789137 MS.gene00618:CDS
GCTAACTCAAAACCTGAGCT+TGG 0.550830 2.1:-75789241 None:intergenic
TCTTCAGAGCAAGGATAAGA+AGG 0.557777 2.1:+75789146 MS.gene00618:CDS
GTCTTACAAGGACGTTACGC+TGG 0.570383 2.1:+75788886 MS.gene00618:CDS
ACGTGCTTACGGTCATTGCT+TGG 0.573817 2.1:+75788954 MS.gene00618:CDS
AAAGCGGTGATCGTCTTACA+AGG 0.580022 2.1:+75788874 MS.gene00618:CDS
TATGCCAACACGTTACACTC+TGG 0.586595 2.1:+75789089 MS.gene00618:CDS
ATCGTGAAGAACTTTGATGA+TGG 0.589985 2.1:+75788922 MS.gene00618:CDS
TCGAAGAAAGGTTCAAAACT+GGG 0.592072 2.1:+75789199 MS.gene00618:CDS
TCCAGCTAAGGTGATCAAGA+AGG 0.605612 2.1:+75788996 MS.gene00618:CDS
ATGACCGTAAGCACGTTCAC+GGG 0.610478 2.1:-75788947 None:intergenic
GATCAAGAAGTATCCAGCTA+AGG 0.616060 2.1:+75788984 MS.gene00618:CDS
CTTTGAAGGAGGCCAAGAAG+AGG 0.631043 2.1:+75789175 MS.gene00618:CDS
ACATCCAGAGTGTAACGTGT+TGG 0.631735 2.1:-75789093 None:intergenic
GAAGGTCACTGCTTTGAAGG+AGG 0.643354 2.1:+75789164 MS.gene00618:CDS
TCAAAACTGGGAAGAACAGG+TGG 0.653226 2.1:+75789211 MS.gene00618:CDS
CTTGCTCTGAAGAACATCAG+TGG 0.671311 2.1:-75789136 None:intergenic
TCTGGATGTTGATTTGAAGG+AGG 0.686222 2.1:+75789107 MS.gene00618:CDS
ATTCTTGAAGCCAAACAAAG+CGG 0.699139 2.1:+75788858 MS.gene00618:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
ATCGTGAAGAACTTTGATGA+TGG + chr2.1:75788922-75788941 MS.gene00618:CDS 35.0%
ATTCTTGAAGCCAAACAAAG+CGG + chr2.1:75788858-75788877 MS.gene00618:CDS 35.0%
TCGAAGAAAGGTTCAAAACT+GGG + chr2.1:75789199-75789218 MS.gene00618:CDS 35.0%
TTCGAAGAAAGGTTCAAAAC+TGG + chr2.1:75789198-75789217 MS.gene00618:CDS 35.0%
! TGTTAAGGCTTTTGTGAAGT+TGG + chr2.1:75789050-75789069 MS.gene00618:CDS 35.0%
TAAGAAGGTCACTGCTTTGA+AGG + chr2.1:75789161-75789180 MS.gene00618:CDS 40.0%
TCTTCAGAGCAAGGATAAGA+AGG + chr2.1:75789146-75789165 MS.gene00618:CDS 40.0%
TGCAAAGAAATCGCGTGTTA+AGG + chr2.1:75789035-75789054 MS.gene00618:CDS 40.0%
!! CACTCTGGATGTTGATTTGA+AGG + chr2.1:75789104-75789123 MS.gene00618:CDS 40.0%
!! GATCAAGAAGTATCCAGCTA+AGG + chr2.1:75788984-75789003 MS.gene00618:CDS 40.0%
!! TCTGGATGTTGATTTGAAGG+AGG + chr2.1:75789107-75789126 MS.gene00618:CDS 40.0%
AAAGCGGTGATCGTCTTACA+AGG + chr2.1:75788874-75788893 MS.gene00618:CDS 45.0%
AATGACCGTAAGCACGTTCA+CGG - chr2.1:75788951-75788970 None:intergenic 45.0%
ACATCCAGAGTGTAACGTGT+TGG - chr2.1:75789096-75789115 None:intergenic 45.0%
CACTGATGTTCTTCAGAGCA+AGG + chr2.1:75789137-75789156 MS.gene00618:CDS 45.0%
CCAAGAAGAGGTTCGAAGAA+AGG + chr2.1:75789187-75789206 MS.gene00618:CDS 45.0%
CCTTTCTTCGAACCTCTTCT+TGG - chr2.1:75789190-75789209 None:intergenic 45.0%
GGTTCAAAACTGGGAAGAAC+AGG + chr2.1:75789208-75789227 MS.gene00618:CDS 45.0%
TATGCCAACACGTTACACTC+TGG + chr2.1:75789089-75789108 MS.gene00618:CDS 45.0%
TCAAAACTGGGAAGAACAGG+TGG + chr2.1:75789211-75789230 MS.gene00618:CDS 45.0%
TCCAGCTAAGGTGATCAAGA+AGG + chr2.1:75788996-75789015 MS.gene00618:CDS 45.0%
TCCTTCTTGATCACCTTAGC+TGG - chr2.1:75789000-75789019 None:intergenic 45.0%
! AAGACGATCACCGCTTTGTT+TGG - chr2.1:75788871-75788890 None:intergenic 45.0%
!! CTTGCTCTGAAGAACATCAG+TGG - chr2.1:75789139-75789158 None:intergenic 45.0%
ACGGTCATTGCTTGGTTGCA+GGG + chr2.1:75788962-75788981 MS.gene00618:CDS 50.0%
ACGTGCTTACGGTCATTGCT+TGG + chr2.1:75788954-75788973 MS.gene00618:CDS 50.0%
AGGACGTTACGCTGGCAAAA+AGG + chr2.1:75788894-75788913 MS.gene00618:CDS 50.0%
ATGACCGTAAGCACGTTCAC+GGG - chr2.1:75788950-75788969 None:intergenic 50.0%
GTCTTACAAGGACGTTACGC+TGG + chr2.1:75788886-75788905 MS.gene00618:CDS 50.0%
TACGGTCATTGCTTGGTTGC+AGG + chr2.1:75788961-75788980 MS.gene00618:CDS 50.0%
! CTTTGAAGGAGGCCAAGAAG+AGG + chr2.1:75789175-75789194 MS.gene00618:CDS 50.0%
!! GAAGGTCACTGCTTTGAAGG+AGG + chr2.1:75789164-75789183 MS.gene00618:CDS 50.0%
GGAACCCGTGAACGTGCTTA+CGG + chr2.1:75788943-75788962 MS.gene00618:CDS 55.0%
GGTGGTTCTTCACCAAGCTC+AGG + chr2.1:75789229-75789248 MS.gene00618:CDS 55.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 75788850 75789257 75788850 ID=MS.gene00618
chr2.1 mRNA 75788850 75789257 75788850 ID=MS.gene00618.t1;Parent=MS.gene00618
chr2.1 exon 75788850 75789257 75788850 ID=MS.gene00618.t1.exon1;Parent=MS.gene00618.t1
chr2.1 CDS 75788850 75789257 75788850 ID=cds.MS.gene00618.t1;Parent=MS.gene00618.t1
Gene Sequence

>MS.gene00618

ATGGTGAAATTCTTGAAGCCAAACAAAGCGGTGATCGTCTTACAAGGACGTTACGCTGGCAAAAAGGCTGTCATCGTGAAGAACTTTGATGATGGAACCCGTGAACGTGCTTACGGTCATTGCTTGGTTGCAGGGATCAAGAAGTATCCAGCTAAGGTGATCAAGAAGGATTCAGCTAAGAAAACTGCAAAGAAATCGCGTGTTAAGGCTTTTGTGAAGTTGGTTAACTATCAGCATCTTATGCCAACACGTTACACTCTGGATGTTGATTTGAAGGAGGTTGTTACCACTGATGTTCTTCAGAGCAAGGATAAGAAGGTCACTGCTTTGAAGGAGGCCAAGAAGAGGTTCGAAGAAAGGTTCAAAACTGGGAAGAACAGGTGGTTCTTCACCAAGCTCAGGTTTTGA
Protein sequence

>MS.gene00618.t1

MVKFLKPNKAVIVLQGRYAGKKAVIVKNFDDGTRERAYGHCLVAGIKKYPAKVIKKDSAKKTAKKSRVKAFVKLVNYQHLMPTRYTLDVDLKEVVTTDVLQSKDKKVTALKEAKKRFEERFKTGKNRWFFTKLRF