AlfalfaGEDB | MS.gene57462

M. sativa cultivar XinJiangDaYe / MS.gene57462

KEGG: K02901
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF01777.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene57462.t1	XP_003594893.2	100	135	0	0	1	135	1	135	1.60E-66	261.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene57462.t1	Q05462	89.6	135	14	0	1	135	1	135	6.0e-62	238.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene57462.t1	G7IG15	100.0	135	0	0	1	135	1	135	1.2e-66	261.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050277	MS.gene57462	0.821033	5.01E-53	-1.69E-46
MS.gene050290	MS.gene57462	0.848878	4.63E-60	-1.69E-46
MS.gene050320	MS.gene57462	0.80958	1.76E-50	-1.69E-46
MS.gene050838	MS.gene57462	0.801027	1.09E-48	-1.69E-46
MS.gene051330	MS.gene57462	0.80743	5.07E-50	-1.69E-46
MS.gene051746	MS.gene57462	0.860272	2.32E-63	-1.69E-46
MS.gene051748	MS.gene57462	0.852055	5.93E-61	-1.69E-46
MS.gene051850	MS.gene57462	0.854806	9.63E-62	-1.69E-46
MS.gene051903	MS.gene57462	0.855708	5.26E-62	-1.69E-46
MS.gene052947	MS.gene57462	0.813234	2.84E-51	-1.69E-46
MS.gene053109	MS.gene57462	0.83451	2.88E-56	-1.69E-46
MS.gene053952	MS.gene57462	0.840233	9.84E-58	-1.69E-46
MS.gene054937	MS.gene57462	0.864737	9.80E-65	-1.69E-46
MS.gene055391	MS.gene57462	0.807386	5.18E-50	-1.69E-46
MS.gene056075	MS.gene57462	0.833043	6.69E-56	-1.69E-46
MS.gene056631	MS.gene57462	0.802801	4.72E-49	-1.69E-46
MS.gene057052	MS.gene57462	0.814663	1.37E-51	-1.69E-46
MS.gene057094	MS.gene57462	0.873358	1.56E-67	-1.69E-46
MS.gene058354	MS.gene57462	0.822216	2.67E-53	-1.69E-46
MS.gene059726	MS.gene57462	0.841817	3.77E-58	-1.69E-46
MS.gene06001	MS.gene57462	0.870479	1.42E-66	-1.69E-46
MS.gene061145	MS.gene57462	0.847752	9.48E-60	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene057706	MS.gene57462	PPI
MS.gene57462	MS.gene073466	PPI
MS.gene005349	MS.gene57462	PPI
MS.gene073466	MS.gene57462	PPI
MS.gene00620	MS.gene57462	PPI
MS.gene57462	MS.gene002051	PPI
MS.gene58693	MS.gene57462	PPI
MS.gene072057	MS.gene57462	PPI
MS.gene047199	MS.gene57462	PPI
MS.gene047317	MS.gene57462	PPI
MS.gene57462	MS.gene00618	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene57462.t1	MTR_2g035930	100.000	135	0	0	1	135	1	135	5.67e-95	270
MS.gene57462.t1	MTR_2g006170	99.259	135	1	0	1	135	1	135	1.94e-94	268
MS.gene57462.t1	MTR_3g048580	64.179	67	15	2	70	135	38	96	2.94e-19	77.0

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene57462.t1	AT4G15000	86.667	135	18	1	135	1	135	7.57e-84	241
MS.gene57462.t1	AT3G22230	86.667	135	18	1	135	1	135	1.19e-83	241
MS.gene57462.t1	AT2G32220	80.000	135	27	1	135	1	135	7.46e-78	226
MS.gene57462.t1	AT4G15000	85.366	123	18	1	123	1	123	4.10e-73	214

Find 35 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CACGATGACGGCTTTCTTCC+CGG	0.266318	2.4:+53374003	None:intergenic
TACGGGCATTGTCTTGTTGC+AGG	0.269826	2.4:-53373947	MS.gene57462:CDS
TCCCGGCGTAGCGTCCTTGT+AGG	0.400507	2.4:+53374020	None:intergenic
GGTTTAAGACTGGGAAGAAT+AGG	0.401208	2.4:-53373700	MS.gene57462:CDS
GGAACTCGCGACCGTGCTTA+CGG	0.402067	2.4:-53373965	MS.gene57462:CDS
TACTGATGTTCTTCAGAGTA+AGG	0.417538	2.4:-53373771	MS.gene57462:CDS
AGGACGATGACGGCTTTGTT+TGG	0.423494	2.4:+53374040	None:intergenic
TAAGAAGGTGACTGCTTTGA+AGG	0.432710	2.4:-53373747	MS.gene57462:CDS
TCCTTCTTGATAACCTTAGC+TGG	0.439811	2.4:+53373911	None:intergenic
CTTCACACGCGATTTCTTCG+CGG	0.451730	2.4:+53373874	None:intergenic
GAACTCGCGACCGTGCTTAC+GGG	0.459112	2.4:-53373964	MS.gene57462:CDS
GGTGGTTCTTTACCAAGCTC+AGG	0.462687	2.4:-53373679	MS.gene57462:CDS
TCCTACAAGGACGCTACGCC+GGG	0.466145	2.4:-53374021	MS.gene57462:CDS
GTCGAAATTCTTCACGATGA+CGG	0.503904	2.4:+53373991	None:intergenic
TTCGAGGAGAGGTTTAAGAC+TGG	0.506310	2.4:-53373710	MS.gene57462:CDS
ACGGGCATTGTCTTGTTGCA+GGG	0.511441	2.4:-53373946	MS.gene57462:CDS
CGCGAAGAAATCGCGTGTGA+AGG	0.529778	2.4:-53373873	MS.gene57462:CDS
AACGAGTCGGCATCAGATGC+TGG	0.530504	2.4:+53373828	None:intergenic
CGAAGAAGAGGTTCGAGGAG+AGG	0.532977	2.4:-53373721	MS.gene57462:CDS
TCCAGCTAAGGTTATCAAGA+AGG	0.538513	2.4:-53373912	MS.gene57462:CDS
TCGAGGAGAGGTTTAAGACT+GGG	0.540939	2.4:-53373709	MS.gene57462:CDS
AACAGCTCAAAACCTGAGCT+TGG	0.543643	2.4:+53373667	None:intergenic
AAAGCCGTCATCGTCCTACA+AGG	0.551105	2.4:-53374034	MS.gene57462:CDS
TCTTGATGTTGATTTGAAAG+AGG	0.559046	2.4:-53373801	MS.gene57462:CDS
TCTTCAGAGTAAGGATAAGA+AGG	0.568239	2.4:-53373762	MS.gene57462:CDS
GTCCTACAAGGACGCTACGC+CGG	0.584899	2.4:-53374022	MS.gene57462:CDS
CAAGACAATGCCCGTAAGCA+CGG	0.587108	2.4:+53373954	None:intergenic
CTTTGAAGGAGGCGAAGAAG+AGG	0.602084	2.4:-53373733	MS.gene57462:CDS
GATCAAGAAGTATCCAGCTA+AGG	0.602579	2.4:-53373924	MS.gene57462:CDS
GGAGGCGAAGAAGAGGTTCG+AGG	0.607573	2.4:-53373726	MS.gene57462:CDS
TTAAGACTGGGAAGAATAGG+TGG	0.614047	2.4:-53373697	MS.gene57462:CDS
GCGTCCTTGTAGGACGATGA+CGG	0.630553	2.4:+53374030	None:intergenic
GAAGGTGACTGCTTTGAAGG+AGG	0.640915	2.4:-53373744	MS.gene57462:CDS
ACATCAAGAGTGTAACGAGT+CGG	0.653533	2.4:+53373815	None:intergenic
ATCGTGAAGAATTTCGACGA+CGG	0.729202	2.4:-53373986	MS.gene57462:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TCTTGATGTTGATTTGAAAG+AGG	-	chr2.4:53373930-53373949	MS.gene57462:CDS	30.0%
	TACTGATGTTCTTCAGAGTA+AGG	-	chr2.4:53373960-53373979	MS.gene57462:CDS	35.0%
	TCTTCAGAGTAAGGATAAGA+AGG	-	chr2.4:53373969-53373988	MS.gene57462:CDS	35.0%
	ATCGTGAAGAATTTCGACGA+CGG	-	chr2.4:53373745-53373764	MS.gene57462:CDS	40.0%
	GTCGAAATTCTTCACGATGA+CGG	+	chr2.4:53373743-53373762	None:intergenic	40.0%
	TAAGAAGGTGACTGCTTTGA+AGG	-	chr2.4:53373984-53374003	MS.gene57462:CDS	40.0%
	TCCAGCTAAGGTTATCAAGA+AGG	-	chr2.4:53373819-53373838	MS.gene57462:CDS	40.0%
	TTAAGACTGGGAAGAATAGG+TGG	-	chr2.4:53374034-53374053	MS.gene57462:CDS	40.0%
!	ACATCAAGAGTGTAACGAGT+CGG	+	chr2.4:53373919-53373938	None:intergenic	40.0%
!	GGTTTAAGACTGGGAAGAAT+AGG	-	chr2.4:53374031-53374050	MS.gene57462:CDS	40.0%
!	TCCTTCTTGATAACCTTAGC+TGG	+	chr2.4:53373823-53373842	None:intergenic	40.0%
!!	GATCAAGAAGTATCCAGCTA+AGG	-	chr2.4:53373807-53373826	MS.gene57462:CDS	40.0%
!	TCGAGGAGAGGTTTAAGACT+GGG	-	chr2.4:53374022-53374041	MS.gene57462:CDS	45.0%
!	TTCGAGGAGAGGTTTAAGAC+TGG	-	chr2.4:53374021-53374040	MS.gene57462:CDS	45.0%
	AAAGCCGTCATCGTCCTACA+AGG	-	chr2.4:53373697-53373716	MS.gene57462:CDS	50.0%
	CAAGACAATGCCCGTAAGCA+CGG	+	chr2.4:53373780-53373799	None:intergenic	50.0%
	CTTCACACGCGATTTCTTCG+CGG	+	chr2.4:53373860-53373879	None:intergenic	50.0%
	GGTGGTTCTTTACCAAGCTC+AGG	-	chr2.4:53374052-53374071	MS.gene57462:CDS	50.0%
!	ACGGGCATTGTCTTGTTGCA+GGG	-	chr2.4:53373785-53373804	MS.gene57462:CDS	50.0%
!	CTTTGAAGGAGGCGAAGAAG+AGG	-	chr2.4:53373998-53374017	MS.gene57462:CDS	50.0%
!	TACGGGCATTGTCTTGTTGC+AGG	-	chr2.4:53373784-53373803	MS.gene57462:CDS	50.0%
!!	AGGACGATGACGGCTTTGTT+TGG	+	chr2.4:53373694-53373713	None:intergenic	50.0%
!!	GAAGGTGACTGCTTTGAAGG+AGG	-	chr2.4:53373987-53374006	MS.gene57462:CDS	50.0%
	AACGAGTCGGCATCAGATGC+TGG	+	chr2.4:53373906-53373925	None:intergenic	55.0%
	CGAAGAAGAGGTTCGAGGAG+AGG	-	chr2.4:53374010-53374029	MS.gene57462:CDS	55.0%
	CGCGAAGAAATCGCGTGTGA+AGG	-	chr2.4:53373858-53373877	MS.gene57462:CDS	55.0%
	GCGTCCTTGTAGGACGATGA+CGG	+	chr2.4:53373704-53373723	None:intergenic	55.0%
!	CACGATGACGGCTTTCTTCC+CGG	+	chr2.4:53373731-53373750	None:intergenic	55.0%
	GAACTCGCGACCGTGCTTAC+GGG	-	chr2.4:53373767-53373786	MS.gene57462:CDS	60.0%
	GGAACTCGCGACCGTGCTTA+CGG	-	chr2.4:53373766-53373785	MS.gene57462:CDS	60.0%
	GGAGGCGAAGAAGAGGTTCG+AGG	-	chr2.4:53374005-53374024	MS.gene57462:CDS	60.0%
	GTCCTACAAGGACGCTACGC+CGG	-	chr2.4:53373709-53373728	MS.gene57462:CDS	60.0%
	TCCTACAAGGACGCTACGCC+GGG	-	chr2.4:53373710-53373729	MS.gene57462:CDS	60.0%
	TCCCGGCGTAGCGTCCTTGT+AGG	+	chr2.4:53373714-53373733	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.4	gene	53373673	53374080	53373673	ID=MS.gene57462
chr2.4	mRNA	53373673	53374080	53373673	ID=MS.gene57462.t1;Parent=MS.gene57462
chr2.4	exon	53373673	53374080	53373673	ID=MS.gene57462.t1.exon1;Parent=MS.gene57462.t1
chr2.4	CDS	53373673	53374080	53373673	ID=cds.MS.gene57462.t1;Parent=MS.gene57462.t1

Gene Sequence

>MS.gene57462

ATGGTGAAATTCTTGAAACCAAACAAAGCCGTCATCGTCCTACAAGGACGCTACGCCGGGAAGAAAGCCGTCATCGTGAAGAATTTCGACGACGGAACTCGCGACCGTGCTTACGGGCATTGTCTTGTTGCAGGGATCAAGAAGTATCCAGCTAAGGTTATCAAGAAGGATTCAGCGAAGAAAACCGCGAAGAAATCGCGTGTGAAGGCTTTCGTGAAGCTTGTTAACTACCAGCATCTGATGCCGACTCGTTACACTCTTGATGTTGATTTGAAAGAGGTTGTTACTACTGATGTTCTTCAGAGTAAGGATAAGAAGGTGACTGCTTTGAAGGAGGCGAAGAAGAGGTTCGAGGAGAGGTTTAAGACTGGGAAGAATAGGTGGTTCTTTACCAAGCTCAGGTTTTGA

Protein sequence

>MS.gene57462.t1

MVKFLKPNKAVIVLQGRYAGKKAVIVKNFDDGTRDRAYGHCLVAGIKKYPAKVIKKDSAKKTAKKSRVKAFVKLVNYQHLMPTRYTLDVDLKEVVTTDVLQSKDKKVTALKEAKKRFEERFKTGKNRWFFTKLRF