AlfalfaGEDB | MS.gene00620

M. sativa cultivar XinJiangDaYe / MS.gene00620

KEGG: K02901
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF01777.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene00620.t1	XP_003592964.1	100	126	0	0	1	126	1	126	2.10E-60	241.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene00620.t1	Q05462	89.7	126	13	0	1	126	1	126	2.7e-56	219.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene00620.t1	B7FH51	100.0	126	0	0	1	126	1	126	1.5e-60	241.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene00620	MS.gene072057	PPI
MS.gene072057	MS.gene00620	PPI
MS.gene047199	MS.gene00620	PPI
MS.gene58693	MS.gene00620	PPI
MS.gene00620	MS.gene047199	PPI
MS.gene00620	MS.gene073466	PPI
MS.gene00620	MS.gene57462	PPI
MS.gene00620	MS.gene002051	PPI
MS.gene00620	MS.gene58693	PPI
MS.gene00620	MS.gene005349	PPI
MS.gene00620	MS.gene047317	PPI
MS.gene00620	MS.gene00618	PPI
MS.gene057706	MS.gene00620	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene00620.t1	MTR_2g006170	100.000	126	0	0	1	126	1	126	1.64e-87	250
MS.gene00620.t1	MTR_2g035930	99.206	126	1	0	1	126	1	126	9.70e-87	248
MS.gene00620.t1	MTR_3g048580	60.345	58	14	2	70	126	38	87	1.73e-12	59.3

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene00620.t1	AT4G15000	84.921	126	19	1	126	1	126	1.85e-75	220
MS.gene00620.t1	AT3G22230	84.921	126	19	1	126	1	126	3.20e-75	219
MS.gene00620.t1	AT4G15000	84.553	123	19	1	123	1	123	5.62e-73	214
MS.gene00620.t1	AT2G32220	79.365	126	26	1	126	1	126	1.56e-70	207

Find 31 sgRNAs with CRISPR-Local

Find 31 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCTTTCTTCGAACCTCTTCT+TGG	0.194010	2.1:-75776202	None:intergenic
TACGGTCATTGCTTGGTTGC+AGG	0.251311	2.1:+75775976	MS.gene00620:CDS
CACTCTGGATGTTGATTTGA+AGG	0.342388	2.1:+75776119	MS.gene00620:CDS
TTCGAAGAAAGGTTCAAAAC+TGG	0.371776	2.1:+75776213	MS.gene00620:CDS
AGGACGTTACGCTGGCAAAA+AGG	0.377725	2.1:+75775909	MS.gene00620:CDS
AAGACGATCACCGCTTTGTT+TGG	0.378594	2.1:-75775883	None:intergenic
TCCTTCTTGATCACCTTAGC+TGG	0.435443	2.1:-75776012	None:intergenic
GGTTCAAAACTGGGAAGAAC+AGG	0.438118	2.1:+75776223	MS.gene00620:CDS
TAAGAAGGTCACTGCTTTGA+AGG	0.444100	2.1:+75776176	MS.gene00620:CDS
TGCAAAGAAATCGCGTGTTA+AGG	0.450525	2.1:+75776050	MS.gene00620:CDS
ACGGTCATTGCTTGGTTGCA+GGG	0.480537	2.1:+75775977	MS.gene00620:CDS
CCAAGAAGAGGTTCGAAGAA+AGG	0.486021	2.1:+75776202	MS.gene00620:CDS
GGAACCCGTGAACGTGCTTA+CGG	0.489757	2.1:+75775958	MS.gene00620:CDS
AATGACCGTAAGCACGTTCA+CGG	0.519577	2.1:-75775963	None:intergenic
CACTGATGTTCTTCAGAGCA+AGG	0.541833	2.1:+75776152	MS.gene00620:CDS
TCTTCAGAGCAAGGATAAGA+AGG	0.557777	2.1:+75776161	MS.gene00620:CDS
GTCTTACAAGGACGTTACGC+TGG	0.570383	2.1:+75775901	MS.gene00620:CDS
ACGTGCTTACGGTCATTGCT+TGG	0.573817	2.1:+75775969	MS.gene00620:CDS
AAAGCGGTGATCGTCTTACA+AGG	0.580022	2.1:+75775889	MS.gene00620:CDS
TATGCCAACACGTTACACTC+TGG	0.586595	2.1:+75776104	MS.gene00620:CDS
ATCGTGAAGAACTTTGATGA+TGG	0.589985	2.1:+75775937	MS.gene00620:CDS
TCGAAGAAAGGTTCAAAACT+GGG	0.592072	2.1:+75776214	MS.gene00620:CDS
TCCAGCTAAGGTGATCAAGA+AGG	0.605612	2.1:+75776011	MS.gene00620:CDS
ATGACCGTAAGCACGTTCAC+GGG	0.610478	2.1:-75775962	None:intergenic
GATCAAGAAGTATCCAGCTA+AGG	0.616060	2.1:+75775999	MS.gene00620:CDS
CTTTGAAGGAGGCCAAGAAG+AGG	0.631043	2.1:+75776190	MS.gene00620:CDS
ACATCCAGAGTGTAACGTGT+TGG	0.631735	2.1:-75776108	None:intergenic
GAAGGTCACTGCTTTGAAGG+AGG	0.643354	2.1:+75776179	MS.gene00620:CDS
CTTGCTCTGAAGAACATCAG+TGG	0.671311	2.1:-75776151	None:intergenic
TCTGGATGTTGATTTGAAGG+AGG	0.686222	2.1:+75776122	MS.gene00620:CDS
ATTCTTGAAGCCAAACAAAG+CGG	0.699139	2.1:+75775873	MS.gene00620:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	ATCGTGAAGAACTTTGATGA+TGG	+	chr2.1:75775937-75775956	MS.gene00620:CDS	35.0%
	ATTCTTGAAGCCAAACAAAG+CGG	+	chr2.1:75775873-75775892	MS.gene00620:CDS	35.0%
	TCGAAGAAAGGTTCAAAACT+GGG	+	chr2.1:75776214-75776233	MS.gene00620:CDS	35.0%
	TTCGAAGAAAGGTTCAAAAC+TGG	+	chr2.1:75776213-75776232	MS.gene00620:CDS	35.0%
!	TGTTAAGGCTTTTGTGAAGT+TGG	+	chr2.1:75776065-75776084	MS.gene00620:CDS	35.0%
	TAAGAAGGTCACTGCTTTGA+AGG	+	chr2.1:75776176-75776195	MS.gene00620:CDS	40.0%
	TCTTCAGAGCAAGGATAAGA+AGG	+	chr2.1:75776161-75776180	MS.gene00620:CDS	40.0%
	TGCAAAGAAATCGCGTGTTA+AGG	+	chr2.1:75776050-75776069	MS.gene00620:CDS	40.0%
!!	CACTCTGGATGTTGATTTGA+AGG	+	chr2.1:75776119-75776138	MS.gene00620:CDS	40.0%
!!	GATCAAGAAGTATCCAGCTA+AGG	+	chr2.1:75775999-75776018	MS.gene00620:CDS	40.0%
!!	TCTGGATGTTGATTTGAAGG+AGG	+	chr2.1:75776122-75776141	MS.gene00620:CDS	40.0%
	AAAGCGGTGATCGTCTTACA+AGG	+	chr2.1:75775889-75775908	MS.gene00620:CDS	45.0%
	AATGACCGTAAGCACGTTCA+CGG	-	chr2.1:75775966-75775985	None:intergenic	45.0%
	ACATCCAGAGTGTAACGTGT+TGG	-	chr2.1:75776111-75776130	None:intergenic	45.0%
	CACTGATGTTCTTCAGAGCA+AGG	+	chr2.1:75776152-75776171	MS.gene00620:CDS	45.0%
	CCAAGAAGAGGTTCGAAGAA+AGG	+	chr2.1:75776202-75776221	MS.gene00620:CDS	45.0%
	CCTTTCTTCGAACCTCTTCT+TGG	-	chr2.1:75776205-75776224	None:intergenic	45.0%
	TATGCCAACACGTTACACTC+TGG	+	chr2.1:75776104-75776123	MS.gene00620:CDS	45.0%
	TCCAGCTAAGGTGATCAAGA+AGG	+	chr2.1:75776011-75776030	MS.gene00620:CDS	45.0%
	TCCTTCTTGATCACCTTAGC+TGG	-	chr2.1:75776015-75776034	None:intergenic	45.0%
!	AAGACGATCACCGCTTTGTT+TGG	-	chr2.1:75775886-75775905	None:intergenic	45.0%
!!	CTTGCTCTGAAGAACATCAG+TGG	-	chr2.1:75776154-75776173	None:intergenic	45.0%
	ACGGTCATTGCTTGGTTGCA+GGG	+	chr2.1:75775977-75775996	MS.gene00620:CDS	50.0%
	ACGTGCTTACGGTCATTGCT+TGG	+	chr2.1:75775969-75775988	MS.gene00620:CDS	50.0%
	AGGACGTTACGCTGGCAAAA+AGG	+	chr2.1:75775909-75775928	MS.gene00620:CDS	50.0%
	ATGACCGTAAGCACGTTCAC+GGG	-	chr2.1:75775965-75775984	None:intergenic	50.0%
	GTCTTACAAGGACGTTACGC+TGG	+	chr2.1:75775901-75775920	MS.gene00620:CDS	50.0%
	TACGGTCATTGCTTGGTTGC+AGG	+	chr2.1:75775976-75775995	MS.gene00620:CDS	50.0%
!	CTTTGAAGGAGGCCAAGAAG+AGG	+	chr2.1:75776190-75776209	MS.gene00620:CDS	50.0%
!!	GAAGGTCACTGCTTTGAAGG+AGG	+	chr2.1:75776179-75776198	MS.gene00620:CDS	50.0%
	GGAACCCGTGAACGTGCTTA+CGG	+	chr2.1:75775958-75775977	MS.gene00620:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.1	gene	75775865	75776244	75775865	ID=MS.gene00620
chr2.1	mRNA	75775865	75776244	75775865	ID=MS.gene00620.t1;Parent=MS.gene00620
chr2.1	exon	75775865	75776244	75775865	ID=MS.gene00620.t1.exon1;Parent=MS.gene00620.t1
chr2.1	CDS	75775865	75776244	75775865	ID=cds.MS.gene00620.t1;Parent=MS.gene00620.t1

Gene Sequence	>MS.gene00620 ATGGTGAAATTCTTGAAGCCAAACAAAGCGGTGATCGTCTTACAAGGACGTTACGCTGGCAAAAAGGCTGTCATCGTGAAGAACTTTGATGATGGAACCCGTGAACGTGCTTACGGTCATTGCTTGGTTGCAGGGATCAAGAAGTATCCAGCTAAGGTGATCAAGAAGGATTCAGCTAAGAAAACTGCAAAGAAATCGCGTGTTAAGGCTTTTGTGAAGTTGGTTAACTATCAGCATCTTATGCCAACACGTTACACTCTGGATGTTGATTTGAAGGAGGTTGTTACCACTGATGTTCTTCAGAGCAAGGATAAGAAGGTCACTGCTTTGAAGGAGGCCAAGAAGAGGTTCGAAGAAAGGTTCAAAACTGGGAAGAACAG
Protein sequence	>MS.gene00620.t1 MVKFLKPNKAVIVLQGRYAGKKAVIVKNFDDGTRERAYGHCLVAGIKKYPAKVIKKDSAKKTAKKSRVKAFVKLVNYQHLMPTRYTLDVDLKEVVTTDVLQSKDKKVTALKEAKKRFEERFKTGKN