AlfalfaGEDB | MS.gene072057

M. sativa cultivar XinJiangDaYe / MS.gene072057

KEGG: K02901
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF01777.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene072057.t1	XP_003592964.1	100	135	0	0	1	135	1	135	2.70E-66	261.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene072057.t1	Q05462	90.4	135	13	0	1	135	1	135	3.5e-62	238.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene072057.t1	B7FH51	100.0	135	0	0	1	135	1	135	2.0e-66	261.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049140	MS.gene072057	0.840903	6.57E-58	-1.69E-46
MS.gene049580	MS.gene072057	0.837644	4.60E-57	-1.69E-46
MS.gene049861	MS.gene072057	0.823008	1.75E-53	-1.69E-46
MS.gene050114	MS.gene072057	0.818585	1.82E-52	-1.69E-46
MS.gene050277	MS.gene072057	0.872155	3.95E-67	-1.69E-46
MS.gene050288	MS.gene072057	0.804525	2.07E-49	-1.69E-46
MS.gene050317	MS.gene072057	0.827281	1.71E-54	-1.69E-46
MS.gene050320	MS.gene072057	0.870193	1.76E-66	-1.69E-46
MS.gene050752	MS.gene072057	0.854744	1.00E-61	-1.69E-46
MS.gene050838	MS.gene072057	0.897077	1.99E-76	-1.69E-46
MS.gene050945	MS.gene072057	0.820532	6.53E-53	-1.69E-46
MS.gene050955	MS.gene072057	0.835431	1.69E-56	-1.69E-46
MS.gene051003	MS.gene072057	0.837305	5.62E-57	-1.69E-46
MS.gene051063	MS.gene072057	0.838651	2.53E-57	-1.69E-46
MS.gene051233	MS.gene072057	0.838366	3.00E-57	-1.69E-46
MS.gene051234	MS.gene072057	0.837048	6.54E-57	-1.69E-46
MS.gene051245	MS.gene072057	0.813112	3.02E-51	-1.69E-46
MS.gene051330	MS.gene072057	0.873391	1.53E-67	-1.69E-46
MS.gene051343	MS.gene072057	0.84137	4.95E-58	-1.69E-46
MS.gene051451	MS.gene072057	0.839348	1.67E-57	-1.69E-46
MS.gene051676	MS.gene072057	0.825958	3.53E-54	-1.69E-46
MS.gene051687	MS.gene072057	0.809584	1.76E-50	-1.69E-46
MS.gene051700	MS.gene072057	0.812063	5.12E-51	-1.69E-46
MS.gene051746	MS.gene072057	0.895011	1.44E-75	-1.69E-46
MS.gene051748	MS.gene072057	0.904222	1.54E-79	-1.69E-46
MS.gene051756	MS.gene072057	0.868956	4.44E-66	-1.69E-46
MS.gene051850	MS.gene072057	0.854059	1.58E-61	-1.69E-46
MS.gene05188	MS.gene072057	0.83295	7.05E-56	-1.69E-46
MS.gene051902	MS.gene072057	0.819901	9.10E-53	-1.69E-46
MS.gene051903	MS.gene072057	0.931162	5.68E-94	-1.69E-46
MS.gene051975	MS.gene072057	0.835402	1.71E-56	-1.69E-46
MS.gene052133	MS.gene072057	0.808076	3.70E-50	-1.69E-46
MS.gene052296	MS.gene072057	0.816104	6.57E-52	-1.69E-46
MS.gene052335	MS.gene072057	0.80136	9.34E-49	-1.69E-46
MS.gene052479	MS.gene072057	0.816055	6.74E-52	-1.69E-46
MS.gene052527	MS.gene072057	0.802341	5.88E-49	-1.69E-46
MS.gene052818	MS.gene072057	-0.857417	1.66E-62	-1.69E-46
MS.gene052947	MS.gene072057	0.89843	5.35E-77	-1.69E-46
MS.gene053058	MS.gene072057	0.84242	2.61E-58	-1.69E-46
MS.gene053109	MS.gene072057	0.870412	1.49E-66	-1.69E-46
MS.gene053564	MS.gene072057	0.830778	2.43E-55	-1.69E-46
MS.gene053786	MS.gene072057	0.814635	1.39E-51	-1.69E-46
MS.gene053952	MS.gene072057	0.892491	1.51E-74	-1.69E-46
MS.gene054403	MS.gene072057	0.855654	5.46E-62	-1.69E-46
MS.gene05469	MS.gene072057	0.847554	1.07E-59	-1.69E-46
MS.gene054937	MS.gene072057	0.805789	1.13E-49	-1.69E-46
MS.gene055174	MS.gene072057	0.80339	3.57E-49	-1.69E-46
MS.gene055311	MS.gene072057	0.806597	7.62E-50	-1.69E-46
MS.gene055424	MS.gene072057	0.861951	7.15E-64	-1.69E-46
MS.gene055555	MS.gene072057	0.818406	1.99E-52	-1.69E-46
MS.gene055603	MS.gene072057	0.838845	2.26E-57	-1.69E-46
MS.gene055808	MS.gene072057	0.815049	1.13E-51	-1.69E-46
MS.gene056075	MS.gene072057	0.879662	1.04E-69	-1.69E-46
MS.gene056091	MS.gene072057	0.83479	2.45E-56	-1.69E-46
MS.gene056403	MS.gene072057	0.834464	2.95E-56	-1.69E-46
MS.gene056580	MS.gene072057	0.827171	1.81E-54	-1.69E-46
MS.gene056601	MS.gene072057	0.807685	4.48E-50	-1.69E-46
MS.gene056605	MS.gene072057	0.832102	1.14E-55	-1.69E-46
MS.gene056631	MS.gene072057	0.871715	5.53E-67	-1.69E-46
MS.gene056752	MS.gene072057	0.824834	6.51E-54	-1.69E-46
MS.gene056896	MS.gene072057	0.828359	9.39E-55	-1.69E-46
MS.gene056986	MS.gene072057	0.856432	3.23E-62	-1.69E-46
MS.gene056992	MS.gene072057	0.81934	1.22E-52	-1.69E-46
MS.gene057057	MS.gene072057	0.822293	2.56E-53	-1.69E-46
MS.gene057094	MS.gene072057	0.923684	1.92E-89	-1.69E-46
MS.gene057234	MS.gene072057	0.873229	1.73E-67	-1.69E-46
MS.gene057452	MS.gene072057	-0.82488	6.35E-54	-1.69E-46
MS.gene057705	MS.gene072057	0.850858	1.29E-60	-1.69E-46
MS.gene05781	MS.gene072057	0.849688	2.75E-60	-1.69E-46
MS.gene057890	MS.gene072057	-0.802439	5.61E-49	-1.69E-46
MS.gene057999	MS.gene072057	0.877087	8.32E-69	-1.69E-46
MS.gene058054	MS.gene072057	0.81715	3.83E-52	-1.69E-46
MS.gene058055	MS.gene072057	0.837409	5.29E-57	-1.69E-46
MS.gene058074	MS.gene072057	0.878888	1.95E-69	-1.69E-46
MS.gene058238	MS.gene072057	0.886069	4.71E-72	-1.69E-46
MS.gene058364	MS.gene072057	0.818983	1.47E-52	-1.69E-46
MS.gene058852	MS.gene072057	0.802821	4.68E-49	-1.69E-46
MS.gene058906	MS.gene072057	0.836368	9.75E-57	-1.69E-46
MS.gene059146	MS.gene072057	0.837437	5.20E-57	-1.69E-46
MS.gene059171	MS.gene072057	0.828711	7.72E-55	-1.69E-46
MS.gene059227	MS.gene072057	0.864542	1.13E-64	-1.69E-46
MS.gene059726	MS.gene072057	0.812551	4.00E-51	-1.69E-46
MS.gene059898	MS.gene072057	0.802323	5.92E-49	-1.69E-46
MS.gene06001	MS.gene072057	0.920064	2.05E-87	-1.69E-46
MS.gene06011	MS.gene072057	0.877734	4.95E-69	-1.69E-46
MS.gene060429	MS.gene072057	0.813961	1.96E-51	-1.69E-46
MS.gene060463	MS.gene072057	0.820479	6.71E-53	-1.69E-46
MS.gene060465	MS.gene072057	0.828505	8.66E-55	-1.69E-46
MS.gene060586	MS.gene072057	0.812311	4.52E-51	-1.69E-46
MS.gene060763	MS.gene072057	0.871797	5.20E-67	-1.69E-46
MS.gene060830	MS.gene072057	0.835578	1.55E-56	-1.69E-46
MS.gene061145	MS.gene072057	0.82721	1.77E-54	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene072057	MS.gene00618	PPI
MS.gene00620	MS.gene072057	PPI
MS.gene072057	MS.gene00620	PPI
MS.gene057706	MS.gene072057	PPI
MS.gene047199	MS.gene072057	PPI
MS.gene58693	MS.gene072057	PPI
MS.gene072057	MS.gene047199	PPI
MS.gene072057	MS.gene073466	PPI
MS.gene072057	MS.gene57462	PPI
MS.gene072057	MS.gene002051	PPI
MS.gene072057	MS.gene58693	PPI
MS.gene072057	MS.gene005349	PPI
MS.gene072057	MS.gene047317	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene072057.t1	MTR_2g006170	100.000	135	0	0	1	135	1	135	4.36e-95	270
MS.gene072057.t1	MTR_2g035930	99.259	135	1	0	1	135	1	135	1.94e-94	268
MS.gene072057.t1	MTR_3g048580	64.179	67	15	2	70	135	38	96	2.91e-19	77.0

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene072057.t1	AT4G15000	85.926	135	19	1	135	1	135	2.61e-83	240
MS.gene072057.t1	AT3G22230	85.926	135	19	1	135	1	135	3.75e-83	240
MS.gene072057.t1	AT2G32220	80.741	135	26	1	135	1	135	1.73e-78	228
MS.gene072057.t1	AT4G15000	84.553	123	19	1	123	1	123	1.40e-72	213

Find 34 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCTTTCTTCGAACCTCTTCT+TGG	0.194010	2.4:-76149659	None:intergenic
TACGGTCATTGCTTGGTTGC+AGG	0.251311	2.4:+76149433	MS.gene072057:CDS
CACTCTGGATGTTGATTTGA+AGG	0.342388	2.4:+76149576	MS.gene072057:CDS
TTCGAAGAAAGGTTCAAAAC+TGG	0.371776	2.4:+76149670	MS.gene072057:CDS
AGGACGTTACGCTGGCAAAA+AGG	0.377725	2.4:+76149366	MS.gene072057:CDS
AAGACGATCACCGCTTTGTT+TGG	0.378594	2.4:-76149340	None:intergenic
TCCTTCTTGATCACCTTAGC+TGG	0.435443	2.4:-76149469	None:intergenic
GGTTCAAAACTGGGAAGAAC+AGG	0.438118	2.4:+76149680	MS.gene072057:CDS
TAAGAAGGTCACTGCTTTGA+AGG	0.444100	2.4:+76149633	MS.gene072057:CDS
TGCAAAGAAATCGCGTGTTA+AGG	0.450525	2.4:+76149507	MS.gene072057:CDS
ACGGTCATTGCTTGGTTGCA+GGG	0.480537	2.4:+76149434	MS.gene072057:CDS
CCAAGAAGAGGTTCGAAGAA+AGG	0.486021	2.4:+76149659	MS.gene072057:CDS
GGAACCCGTGAACGTGCTTA+CGG	0.489757	2.4:+76149415	MS.gene072057:CDS
GGTGGTTCTTCACCAAGCTC+AGG	0.503030	2.4:+76149701	MS.gene072057:CDS
AATGACCGTAAGCACGTTCA+CGG	0.519577	2.4:-76149420	None:intergenic
CACTGATGTTCTTCAGAGCA+AGG	0.541833	2.4:+76149609	MS.gene072057:CDS
GCTAACTCAAAACCTGAGCT+TGG	0.550830	2.4:-76149713	None:intergenic
TCTTCAGAGCAAGGATAAGA+AGG	0.557777	2.4:+76149618	MS.gene072057:CDS
GTCTTACAAGGACGTTACGC+TGG	0.570383	2.4:+76149358	MS.gene072057:CDS
ACGTGCTTACGGTCATTGCT+TGG	0.573817	2.4:+76149426	MS.gene072057:CDS
AAAGCGGTGATCGTCTTACA+AGG	0.580022	2.4:+76149346	MS.gene072057:CDS
TATGCCAACACGTTACACTC+TGG	0.586595	2.4:+76149561	MS.gene072057:CDS
ATCGTGAAGAACTTTGATGA+TGG	0.589985	2.4:+76149394	MS.gene072057:CDS
TCGAAGAAAGGTTCAAAACT+GGG	0.592072	2.4:+76149671	MS.gene072057:CDS
TCCAGCTAAGGTGATCAAGA+AGG	0.605612	2.4:+76149468	MS.gene072057:CDS
ATGACCGTAAGCACGTTCAC+GGG	0.610478	2.4:-76149419	None:intergenic
GATCAAGAAGTATCCAGCTA+AGG	0.616060	2.4:+76149456	MS.gene072057:CDS
CTTTGAAGGAGGCCAAGAAG+AGG	0.631043	2.4:+76149647	MS.gene072057:CDS
ACATCCAGAGTGTAACGTGT+TGG	0.631735	2.4:-76149565	None:intergenic
GAAGGTCACTGCTTTGAAGG+AGG	0.643354	2.4:+76149636	MS.gene072057:CDS
TCAAAACTGGGAAGAACAGG+TGG	0.653226	2.4:+76149683	MS.gene072057:CDS
CTTGCTCTGAAGAACATCAG+TGG	0.671311	2.4:-76149608	None:intergenic
TCTGGATGTTGATTTGAAGG+AGG	0.686222	2.4:+76149579	MS.gene072057:CDS
ATTCTTGAAGCCAAACAAAG+CGG	0.699139	2.4:+76149330	MS.gene072057:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	ATCGTGAAGAACTTTGATGA+TGG	+	chr2.4:76149394-76149413	MS.gene072057:CDS	35.0%
	ATTCTTGAAGCCAAACAAAG+CGG	+	chr2.4:76149330-76149349	MS.gene072057:CDS	35.0%
	TCGAAGAAAGGTTCAAAACT+GGG	+	chr2.4:76149671-76149690	MS.gene072057:CDS	35.0%
	TTCGAAGAAAGGTTCAAAAC+TGG	+	chr2.4:76149670-76149689	MS.gene072057:CDS	35.0%
!	TGTTAAGGCTTTTGTGAAGT+TGG	+	chr2.4:76149522-76149541	MS.gene072057:CDS	35.0%
	TAAGAAGGTCACTGCTTTGA+AGG	+	chr2.4:76149633-76149652	MS.gene072057:CDS	40.0%
	TCTTCAGAGCAAGGATAAGA+AGG	+	chr2.4:76149618-76149637	MS.gene072057:CDS	40.0%
	TGCAAAGAAATCGCGTGTTA+AGG	+	chr2.4:76149507-76149526	MS.gene072057:CDS	40.0%
!!	CACTCTGGATGTTGATTTGA+AGG	+	chr2.4:76149576-76149595	MS.gene072057:CDS	40.0%
!!	GATCAAGAAGTATCCAGCTA+AGG	+	chr2.4:76149456-76149475	MS.gene072057:CDS	40.0%
!!	TCTGGATGTTGATTTGAAGG+AGG	+	chr2.4:76149579-76149598	MS.gene072057:CDS	40.0%
	AAAGCGGTGATCGTCTTACA+AGG	+	chr2.4:76149346-76149365	MS.gene072057:CDS	45.0%
	AATGACCGTAAGCACGTTCA+CGG	-	chr2.4:76149423-76149442	None:intergenic	45.0%
	ACATCCAGAGTGTAACGTGT+TGG	-	chr2.4:76149568-76149587	None:intergenic	45.0%
	CACTGATGTTCTTCAGAGCA+AGG	+	chr2.4:76149609-76149628	MS.gene072057:CDS	45.0%
	CCAAGAAGAGGTTCGAAGAA+AGG	+	chr2.4:76149659-76149678	MS.gene072057:CDS	45.0%
	CCTTTCTTCGAACCTCTTCT+TGG	-	chr2.4:76149662-76149681	None:intergenic	45.0%
	GGTTCAAAACTGGGAAGAAC+AGG	+	chr2.4:76149680-76149699	MS.gene072057:CDS	45.0%
	TATGCCAACACGTTACACTC+TGG	+	chr2.4:76149561-76149580	MS.gene072057:CDS	45.0%
	TCAAAACTGGGAAGAACAGG+TGG	+	chr2.4:76149683-76149702	MS.gene072057:CDS	45.0%
	TCCAGCTAAGGTGATCAAGA+AGG	+	chr2.4:76149468-76149487	MS.gene072057:CDS	45.0%
	TCCTTCTTGATCACCTTAGC+TGG	-	chr2.4:76149472-76149491	None:intergenic	45.0%
!	AAGACGATCACCGCTTTGTT+TGG	-	chr2.4:76149343-76149362	None:intergenic	45.0%
!!	CTTGCTCTGAAGAACATCAG+TGG	-	chr2.4:76149611-76149630	None:intergenic	45.0%
	ACGGTCATTGCTTGGTTGCA+GGG	+	chr2.4:76149434-76149453	MS.gene072057:CDS	50.0%
	ACGTGCTTACGGTCATTGCT+TGG	+	chr2.4:76149426-76149445	MS.gene072057:CDS	50.0%
	AGGACGTTACGCTGGCAAAA+AGG	+	chr2.4:76149366-76149385	MS.gene072057:CDS	50.0%
	ATGACCGTAAGCACGTTCAC+GGG	-	chr2.4:76149422-76149441	None:intergenic	50.0%
	GTCTTACAAGGACGTTACGC+TGG	+	chr2.4:76149358-76149377	MS.gene072057:CDS	50.0%
	TACGGTCATTGCTTGGTTGC+AGG	+	chr2.4:76149433-76149452	MS.gene072057:CDS	50.0%
!	CTTTGAAGGAGGCCAAGAAG+AGG	+	chr2.4:76149647-76149666	MS.gene072057:CDS	50.0%
!!	GAAGGTCACTGCTTTGAAGG+AGG	+	chr2.4:76149636-76149655	MS.gene072057:CDS	50.0%
	GGAACCCGTGAACGTGCTTA+CGG	+	chr2.4:76149415-76149434	MS.gene072057:CDS	55.0%
	GGTGGTTCTTCACCAAGCTC+AGG	+	chr2.4:76149701-76149720	MS.gene072057:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.4	gene	76149322	76149729	76149322	ID=MS.gene072057
chr2.4	mRNA	76149322	76149729	76149322	ID=MS.gene072057.t1;Parent=MS.gene072057
chr2.4	exon	76149322	76149729	76149322	ID=MS.gene072057.t1.exon1;Parent=MS.gene072057.t1
chr2.4	CDS	76149322	76149729	76149322	ID=cds.MS.gene072057.t1;Parent=MS.gene072057.t1

Gene Sequence

>MS.gene072057

ATGGTGAAATTCTTGAAGCCAAACAAAGCGGTGATCGTCTTACAAGGACGTTACGCTGGCAAAAAGGCTGTCATCGTGAAGAACTTTGATGATGGAACCCGTGAACGTGCTTACGGTCATTGCTTGGTTGCAGGGATCAAGAAGTATCCAGCTAAGGTGATCAAGAAGGATTCAGCTAAGAAAACTGCAAAGAAATCGCGTGTTAAGGCTTTTGTGAAGTTGGTTAACTATCAGCATCTTATGCCAACACGTTACACTCTGGATGTTGATTTGAAGGAGGTTGTTACCACTGATGTTCTTCAGAGCAAGGATAAGAAGGTCACTGCTTTGAAGGAGGCCAAGAAGAGGTTCGAAGAAAGGTTCAAAACTGGGAAGAACAGGTGGTTCTTCACCAAGCTCAGGTTTTGA

Protein sequence

>MS.gene072057.t1

MVKFLKPNKAVIVLQGRYAGKKAVIVKNFDDGTRERAYGHCLVAGIKKYPAKVIKKDSAKKTAKKSRVKAFVKLVNYQHLMPTRYTLDVDLKEVVTTDVLQSKDKKVTALKEAKKRFEERFKTGKNRWFFTKLRF