AlfalfaGEDB | MS.gene58693

M. sativa cultivar XinJiangDaYe / MS.gene58693

KEGG: K02901
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF01777.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58693.t1	XP_003594893.2	100	135	0	0	1	135	1	135	1.60E-66	261.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58693.t1	Q05462	89.6	135	14	0	1	135	1	135	6.0e-62	238.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58693.t1	G7IG15	100.0	135	0	0	1	135	1	135	1.2e-66	261.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050277	MS.gene58693	0.853085	3.02E-61	-1.69E-46
MS.gene050290	MS.gene58693	0.83406	3.73E-56	-1.69E-46
MS.gene050320	MS.gene58693	0.822936	1.81E-53	-1.69E-46
MS.gene050838	MS.gene58693	0.826425	2.73E-54	-1.69E-46
MS.gene051063	MS.gene58693	0.825935	3.57E-54	-1.69E-46
MS.gene051330	MS.gene58693	0.831862	1.31E-55	-1.69E-46
MS.gene051687	MS.gene58693	0.802625	5.13E-49	-1.69E-46
MS.gene051746	MS.gene58693	0.886853	2.38E-72	-1.69E-46
MS.gene051748	MS.gene58693	0.875587	2.74E-68	-1.69E-46
MS.gene051850	MS.gene58693	0.875546	2.83E-68	-1.69E-46
MS.gene051903	MS.gene58693	0.876042	1.91E-68	-1.69E-46
MS.gene052947	MS.gene58693	0.855616	5.60E-62	-1.69E-46
MS.gene053109	MS.gene58693	0.852107	5.73E-61	-1.69E-46
MS.gene053952	MS.gene58693	0.816254	6.08E-52	-1.69E-46
MS.gene054937	MS.gene58693	0.852552	4.28E-61	-1.69E-46
MS.gene055391	MS.gene58693	0.804445	2.15E-49	-1.69E-46
MS.gene055424	MS.gene58693	0.81206	5.13E-51	-1.69E-46
MS.gene056075	MS.gene58693	0.827367	1.63E-54	-1.69E-46
MS.gene056601	MS.gene58693	0.824967	6.06E-54	-1.69E-46
MS.gene056631	MS.gene58693	0.837658	4.56E-57	-1.69E-46
MS.gene057094	MS.gene58693	0.891895	2.62E-74	-1.69E-46
MS.gene057206	MS.gene58693	0.807469	4.98E-50	-1.69E-46
MS.gene057234	MS.gene58693	0.830784	2.42E-55	-1.69E-46
MS.gene058238	MS.gene58693	0.834756	2.49E-56	-1.69E-46
MS.gene058354	MS.gene58693	0.819001	1.46E-52	-1.69E-46
MS.gene058906	MS.gene58693	0.821091	4.85E-53	-1.69E-46
MS.gene059726	MS.gene58693	0.825956	3.53E-54	-1.69E-46
MS.gene059898	MS.gene58693	0.805551	1.26E-49	-1.69E-46
MS.gene06001	MS.gene58693	0.895821	6.65E-76	-1.69E-46
MS.gene060463	MS.gene58693	0.808576	2.89E-50	-1.69E-46
MS.gene060465	MS.gene58693	0.81455	1.46E-51	-1.69E-46
MS.gene060702	MS.gene58693	0.812461	4.19E-51	-1.69E-46
MS.gene061145	MS.gene58693	0.844874	5.77E-59	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene58693	MS.gene005349	PPI
MS.gene58693	MS.gene00620	PPI
MS.gene057706	MS.gene58693	PPI
MS.gene58693	MS.gene047199	PPI
MS.gene00620	MS.gene58693	PPI
MS.gene58693	MS.gene072057	PPI
MS.gene58693	MS.gene073466	PPI
MS.gene58693	MS.gene57462	PPI
MS.gene58693	MS.gene002051	PPI
MS.gene58693	MS.gene047317	PPI
MS.gene072057	MS.gene58693	PPI
MS.gene047199	MS.gene58693	PPI
MS.gene58693	MS.gene00618	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58693.t1	MTR_2g035930	100.000	135	0	0	1	135	1	135	5.67e-95	270
MS.gene58693.t1	MTR_2g006170	99.259	135	1	0	1	135	1	135	1.94e-94	268
MS.gene58693.t1	MTR_3g048580	64.179	67	15	2	70	135	38	96	2.94e-19	77.0

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58693.t1	AT4G15000	86.667	135	18	1	135	1	135	7.57e-84	241
MS.gene58693.t1	AT3G22230	86.667	135	18	1	135	1	135	1.19e-83	241
MS.gene58693.t1	AT2G32220	80.000	135	27	1	135	1	135	7.46e-78	226
MS.gene58693.t1	AT4G15000	85.366	123	18	1	123	1	123	4.10e-73	214

Find 36 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CACGATGACGGCTTTCTTCC+CGG	0.266318	2.3:+53248015	None:intergenic
TACGGGCATTGTCTTGTTGC+AGG	0.269826	2.3:-53247959	MS.gene58693:CDS
CACTCTTGATGTTGATTTGA+AGG	0.356264	2.3:-53247816	MS.gene58693:CDS
TCCCGGCGTAGCGTCCTTGT+AGG	0.400507	2.3:+53248032	None:intergenic
GGTTTAAGACTGGGAAGAAT+AGG	0.401208	2.3:-53247712	MS.gene58693:CDS
GGAACTCGCGACCGTGCTTA+CGG	0.402067	2.3:-53247977	MS.gene58693:CDS
AGGACGATGACGGCTTTGTT+TGG	0.406607	2.3:+53248052	None:intergenic
TACTGATGTTCTTCAGAGTA+AGG	0.417538	2.3:-53247783	MS.gene58693:CDS
TAAGAAGGTGACTGCTTTGA+AGG	0.432710	2.3:-53247759	MS.gene58693:CDS
TCCTTCTTGATAACCTTAGC+TGG	0.439811	2.3:+53247923	None:intergenic
CTTCACACGCGATTTCTTCG+CGG	0.451730	2.3:+53247886	None:intergenic
GAACTCGCGACCGTGCTTAC+GGG	0.459112	2.3:-53247976	MS.gene58693:CDS
GGTGGTTCTTTACCAAGCTC+AGG	0.462687	2.3:-53247691	MS.gene58693:CDS
TCCTACAAGGACGCTACGCC+GGG	0.466145	2.3:-53248033	MS.gene58693:CDS
GTCGAAATTCTTCACGATGA+CGG	0.503904	2.3:+53248003	None:intergenic
TTCGAGGAGAGGTTTAAGAC+TGG	0.506310	2.3:-53247722	MS.gene58693:CDS
ACGGGCATTGTCTTGTTGCA+GGG	0.511441	2.3:-53247958	MS.gene58693:CDS
CGCGAAGAAATCGCGTGTGA+AGG	0.529778	2.3:-53247885	MS.gene58693:CDS
AACGAGTCGGCATCAGATGC+TGG	0.530504	2.3:+53247840	None:intergenic
CGAAGAAGAGGTTCGAGGAG+AGG	0.532977	2.3:-53247733	MS.gene58693:CDS
TCCAGCTAAGGTTATCAAGA+AGG	0.538513	2.3:-53247924	MS.gene58693:CDS
TCGAGGAGAGGTTTAAGACT+GGG	0.540939	2.3:-53247721	MS.gene58693:CDS
AACAGCTCAAAACCTGAGCT+TGG	0.543643	2.3:+53247679	None:intergenic
AAAGCCGTCATCGTCCTACA+AGG	0.551105	2.3:-53248046	MS.gene58693:CDS
TCTTCAGAGTAAGGATAAGA+AGG	0.568239	2.3:-53247774	MS.gene58693:CDS
GTCCTACAAGGACGCTACGC+CGG	0.584899	2.3:-53248034	MS.gene58693:CDS
CAAGACAATGCCCGTAAGCA+CGG	0.587108	2.3:+53247966	None:intergenic
CTTTGAAGGAGGCGAAGAAG+AGG	0.602084	2.3:-53247745	MS.gene58693:CDS
GATCAAGAAGTATCCAGCTA+AGG	0.602579	2.3:-53247936	MS.gene58693:CDS
GGAGGCGAAGAAGAGGTTCG+AGG	0.607573	2.3:-53247738	MS.gene58693:CDS
TTAAGACTGGGAAGAATAGG+TGG	0.614047	2.3:-53247709	MS.gene58693:CDS
GCGTCCTTGTAGGACGATGA+CGG	0.630553	2.3:+53248042	None:intergenic
GAAGGTGACTGCTTTGAAGG+AGG	0.640915	2.3:-53247756	MS.gene58693:CDS
ACATCAAGAGTGTAACGAGT+CGG	0.653533	2.3:+53247827	None:intergenic
TCTTGATGTTGATTTGAAGG+AGG	0.672741	2.3:-53247813	MS.gene58693:CDS
ATCGTGAAGAATTTCGACGA+CGG	0.729202	2.3:-53247998	MS.gene58693:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TACTGATGTTCTTCAGAGTA+AGG	-	chr2.3:53247972-53247991	MS.gene58693:CDS	35.0%
	TCTTCAGAGTAAGGATAAGA+AGG	-	chr2.3:53247981-53248000	MS.gene58693:CDS	35.0%
!!	CACTCTTGATGTTGATTTGA+AGG	-	chr2.3:53247939-53247958	MS.gene58693:CDS	35.0%
!!	TCTTGATGTTGATTTGAAGG+AGG	-	chr2.3:53247942-53247961	MS.gene58693:CDS	35.0%
	ATCGTGAAGAATTTCGACGA+CGG	-	chr2.3:53247757-53247776	MS.gene58693:CDS	40.0%
	GTCGAAATTCTTCACGATGA+CGG	+	chr2.3:53247755-53247774	None:intergenic	40.0%
	TAAGAAGGTGACTGCTTTGA+AGG	-	chr2.3:53247996-53248015	MS.gene58693:CDS	40.0%
	TCCAGCTAAGGTTATCAAGA+AGG	-	chr2.3:53247831-53247850	MS.gene58693:CDS	40.0%
	TTAAGACTGGGAAGAATAGG+TGG	-	chr2.3:53248046-53248065	MS.gene58693:CDS	40.0%
!	ACATCAAGAGTGTAACGAGT+CGG	+	chr2.3:53247931-53247950	None:intergenic	40.0%
!	GGTTTAAGACTGGGAAGAAT+AGG	-	chr2.3:53248043-53248062	MS.gene58693:CDS	40.0%
!	TCCTTCTTGATAACCTTAGC+TGG	+	chr2.3:53247835-53247854	None:intergenic	40.0%
!!	GATCAAGAAGTATCCAGCTA+AGG	-	chr2.3:53247819-53247838	MS.gene58693:CDS	40.0%
!	TCGAGGAGAGGTTTAAGACT+GGG	-	chr2.3:53248034-53248053	MS.gene58693:CDS	45.0%
!	TTCGAGGAGAGGTTTAAGAC+TGG	-	chr2.3:53248033-53248052	MS.gene58693:CDS	45.0%
	AAAGCCGTCATCGTCCTACA+AGG	-	chr2.3:53247709-53247728	MS.gene58693:CDS	50.0%
	CAAGACAATGCCCGTAAGCA+CGG	+	chr2.3:53247792-53247811	None:intergenic	50.0%
	CTTCACACGCGATTTCTTCG+CGG	+	chr2.3:53247872-53247891	None:intergenic	50.0%
	GGTGGTTCTTTACCAAGCTC+AGG	-	chr2.3:53248064-53248083	MS.gene58693:CDS	50.0%
!	ACGGGCATTGTCTTGTTGCA+GGG	-	chr2.3:53247797-53247816	MS.gene58693:CDS	50.0%
!	CTTTGAAGGAGGCGAAGAAG+AGG	-	chr2.3:53248010-53248029	MS.gene58693:CDS	50.0%
!	TACGGGCATTGTCTTGTTGC+AGG	-	chr2.3:53247796-53247815	MS.gene58693:CDS	50.0%
!!	AGGACGATGACGGCTTTGTT+TGG	+	chr2.3:53247706-53247725	None:intergenic	50.0%
!!	GAAGGTGACTGCTTTGAAGG+AGG	-	chr2.3:53247999-53248018	MS.gene58693:CDS	50.0%
	AACGAGTCGGCATCAGATGC+TGG	+	chr2.3:53247918-53247937	None:intergenic	55.0%
	CGAAGAAGAGGTTCGAGGAG+AGG	-	chr2.3:53248022-53248041	MS.gene58693:CDS	55.0%
	CGCGAAGAAATCGCGTGTGA+AGG	-	chr2.3:53247870-53247889	MS.gene58693:CDS	55.0%
	GCGTCCTTGTAGGACGATGA+CGG	+	chr2.3:53247716-53247735	None:intergenic	55.0%
!	CACGATGACGGCTTTCTTCC+CGG	+	chr2.3:53247743-53247762	None:intergenic	55.0%
	GAACTCGCGACCGTGCTTAC+GGG	-	chr2.3:53247779-53247798	MS.gene58693:CDS	60.0%
	GGAACTCGCGACCGTGCTTA+CGG	-	chr2.3:53247778-53247797	MS.gene58693:CDS	60.0%
	GGAGGCGAAGAAGAGGTTCG+AGG	-	chr2.3:53248017-53248036	MS.gene58693:CDS	60.0%
	GTCCTACAAGGACGCTACGC+CGG	-	chr2.3:53247721-53247740	MS.gene58693:CDS	60.0%
	TCCTACAAGGACGCTACGCC+GGG	-	chr2.3:53247722-53247741	MS.gene58693:CDS	60.0%
	TCCCGGCGTAGCGTCCTTGT+AGG	+	chr2.3:53247726-53247745	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.3	gene	53247685	53248092	53247685	ID=MS.gene58693
chr2.3	mRNA	53247685	53248092	53247685	ID=MS.gene58693.t1;Parent=MS.gene58693
chr2.3	exon	53247685	53248092	53247685	ID=MS.gene58693.t1.exon1;Parent=MS.gene58693.t1
chr2.3	CDS	53247685	53248092	53247685	ID=cds.MS.gene58693.t1;Parent=MS.gene58693.t1

Gene Sequence

>MS.gene58693

ATGGTGAAATTCTTGAAGCCAAACAAAGCCGTCATCGTCCTACAAGGACGCTACGCCGGGAAGAAAGCCGTCATCGTGAAGAATTTCGACGACGGAACTCGCGACCGTGCTTACGGGCATTGTCTTGTTGCAGGGATCAAGAAGTATCCAGCTAAGGTTATCAAGAAGGATTCAGCGAAGAAAACCGCGAAGAAATCGCGTGTGAAGGCTTTCGTGAAGCTTGTTAACTACCAGCATCTGATGCCGACTCGTTACACTCTTGATGTTGATTTGAAGGAGGTTGTTACTACTGATGTTCTTCAGAGTAAGGATAAGAAGGTGACTGCTTTGAAGGAGGCGAAGAAGAGGTTCGAGGAGAGGTTTAAGACTGGGAAGAATAGGTGGTTCTTTACCAAGCTCAGGTTTTGA

Protein sequence

>MS.gene58693.t1

MVKFLKPNKAVIVLQGRYAGKKAVIVKNFDDGTRDRAYGHCLVAGIKKYPAKVIKKDSAKKTAKKSRVKAFVKLVNYQHLMPTRYTLDVDLKEVVTTDVLQSKDKKVTALKEAKKRFEERFKTGKNRWFFTKLRF