AlfalfaGEDB | MS.gene047317

M. sativa cultivar XinJiangDaYe / MS.gene047317

KEGG: K02901
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF01777.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene047317.t1	XP_003592964.1	100	135	0	0	1	135	1	135	2.70E-66	261.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene047317.t1	Q05462	90.4	135	13	0	1	135	1	135	3.5e-62	238.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene047317.t1	B7FH51	100.0	135	0	0	1	135	1	135	2.0e-66	261.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene047317	MS.gene005349	PPI
MS.gene047317	MS.gene00618	PPI
MS.gene057706	MS.gene047317	PPI
MS.gene005349	MS.gene047317	PPI
MS.gene00620	MS.gene047317	PPI
MS.gene047317	MS.gene073466	PPI
MS.gene047317	MS.gene57462	PPI
MS.gene047317	MS.gene002051	PPI
MS.gene58693	MS.gene047317	PPI
MS.gene072057	MS.gene047317	PPI
MS.gene047199	MS.gene047317	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene047317.t1	MTR_2g006170	100.000	135	0	0	1	135	1	135	4.36e-95	270
MS.gene047317.t1	MTR_2g035930	99.259	135	1	0	1	135	1	135	1.94e-94	268
MS.gene047317.t1	MTR_3g048580	64.179	67	15	2	70	135	38	96	2.91e-19	77.0

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene047317.t1	AT4G15000	85.926	135	19	1	135	1	135	2.61e-83	240
MS.gene047317.t1	AT3G22230	85.926	135	19	1	135	1	135	3.75e-83	240
MS.gene047317.t1	AT2G32220	80.741	135	26	1	135	1	135	1.73e-78	228
MS.gene047317.t1	AT4G15000	84.553	123	19	1	123	1	123	1.40e-72	213

Find 34 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCTTTCTTCGAACCTCTTCT+TGG	0.194010	2.3:-75790432	None:intergenic
TACGGTCATTGCTTGGTTGC+AGG	0.251311	2.3:+75790206	MS.gene047317:CDS
CACTCTGGATGTTGATTTGA+AGG	0.342388	2.3:+75790349	MS.gene047317:CDS
TTCGAAGAAAGGTTCAAAAC+TGG	0.371776	2.3:+75790443	MS.gene047317:CDS
AGGACGTTACGCTGGCAAAA+AGG	0.377725	2.3:+75790139	MS.gene047317:CDS
AAGACGATCACCGCTTTGTT+TGG	0.378594	2.3:-75790113	None:intergenic
CACTGATGTTCTTCAGAGTA+AGG	0.400378	2.3:+75790382	MS.gene047317:CDS
TCCTTCTTGATCACCTTAGC+TGG	0.435443	2.3:-75790242	None:intergenic
GGTTCAAAACTGGGAAGAAC+AGG	0.438118	2.3:+75790453	MS.gene047317:CDS
TAAGAAGGTCACTGCTTTGA+AGG	0.444100	2.3:+75790406	MS.gene047317:CDS
TGCAAAGAAATCGCGTGTTA+AGG	0.450525	2.3:+75790280	MS.gene047317:CDS
ACGGTCATTGCTTGGTTGCA+GGG	0.480537	2.3:+75790207	MS.gene047317:CDS
CCAAGAAGAGGTTCGAAGAA+AGG	0.486021	2.3:+75790432	MS.gene047317:CDS
GGAACCCGTGAACGTGCTTA+CGG	0.489757	2.3:+75790188	MS.gene047317:CDS
GGTGGTTCTTCACCAAGCTC+AGG	0.503030	2.3:+75790474	MS.gene047317:CDS
AATGACCGTAAGCACGTTCA+CGG	0.519577	2.3:-75790193	None:intergenic
GCTAACTCAAAACCTGAGCT+TGG	0.550830	2.3:-75790486	None:intergenic
TCTTCAGAGTAAGGATAAGA+AGG	0.568239	2.3:+75790391	MS.gene047317:CDS
GTCTTACAAGGACGTTACGC+TGG	0.570383	2.3:+75790131	MS.gene047317:CDS
ACGTGCTTACGGTCATTGCT+TGG	0.573817	2.3:+75790199	MS.gene047317:CDS
AAAGCGGTGATCGTCTTACA+AGG	0.580022	2.3:+75790119	MS.gene047317:CDS
TATGCCAACACGTTACACTC+TGG	0.586595	2.3:+75790334	MS.gene047317:CDS
ATCGTGAAGAACTTTGATGA+TGG	0.589985	2.3:+75790167	MS.gene047317:CDS
TCGAAGAAAGGTTCAAAACT+GGG	0.592072	2.3:+75790444	MS.gene047317:CDS
TCCAGCTAAGGTGATCAAGA+AGG	0.605612	2.3:+75790241	MS.gene047317:CDS
ATGACCGTAAGCACGTTCAC+GGG	0.610478	2.3:-75790192	None:intergenic
GATCAAGAAGTATCCAGCTA+AGG	0.616060	2.3:+75790229	MS.gene047317:CDS
CTTTGAAGGAGGCCAAGAAG+AGG	0.631043	2.3:+75790420	MS.gene047317:CDS
ACATCCAGAGTGTAACGTGT+TGG	0.631735	2.3:-75790338	None:intergenic
GAAGGTCACTGCTTTGAAGG+AGG	0.643354	2.3:+75790409	MS.gene047317:CDS
TCAAAACTGGGAAGAACAGG+TGG	0.653226	2.3:+75790456	MS.gene047317:CDS
TCTGGATGTTGATTTGAAGG+AGG	0.686222	2.3:+75790352	MS.gene047317:CDS
CTTACTCTGAAGAACATCAG+TGG	0.694910	2.3:-75790381	None:intergenic
ATTCTTGAAGCCAAACAAAG+CGG	0.699139	2.3:+75790103	MS.gene047317:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	ATCGTGAAGAACTTTGATGA+TGG	+	chr2.3:75790167-75790186	MS.gene047317:CDS	35.0%
	ATTCTTGAAGCCAAACAAAG+CGG	+	chr2.3:75790103-75790122	MS.gene047317:CDS	35.0%
	TCGAAGAAAGGTTCAAAACT+GGG	+	chr2.3:75790444-75790463	MS.gene047317:CDS	35.0%
	TCTTCAGAGTAAGGATAAGA+AGG	+	chr2.3:75790391-75790410	MS.gene047317:CDS	35.0%
	TTCGAAGAAAGGTTCAAAAC+TGG	+	chr2.3:75790443-75790462	MS.gene047317:CDS	35.0%
!	TGTTAAGGCTTTTGTGAAGT+TGG	+	chr2.3:75790295-75790314	MS.gene047317:CDS	35.0%
	CACTGATGTTCTTCAGAGTA+AGG	+	chr2.3:75790382-75790401	MS.gene047317:CDS	40.0%
	TAAGAAGGTCACTGCTTTGA+AGG	+	chr2.3:75790406-75790425	MS.gene047317:CDS	40.0%
	TGCAAAGAAATCGCGTGTTA+AGG	+	chr2.3:75790280-75790299	MS.gene047317:CDS	40.0%
!	CTTACTCTGAAGAACATCAG+TGG	-	chr2.3:75790384-75790403	None:intergenic	40.0%
!!	CACTCTGGATGTTGATTTGA+AGG	+	chr2.3:75790349-75790368	MS.gene047317:CDS	40.0%
!!	GATCAAGAAGTATCCAGCTA+AGG	+	chr2.3:75790229-75790248	MS.gene047317:CDS	40.0%
!!	TCTGGATGTTGATTTGAAGG+AGG	+	chr2.3:75790352-75790371	MS.gene047317:CDS	40.0%
	AAAGCGGTGATCGTCTTACA+AGG	+	chr2.3:75790119-75790138	MS.gene047317:CDS	45.0%
	AATGACCGTAAGCACGTTCA+CGG	-	chr2.3:75790196-75790215	None:intergenic	45.0%
	ACATCCAGAGTGTAACGTGT+TGG	-	chr2.3:75790341-75790360	None:intergenic	45.0%
	CCAAGAAGAGGTTCGAAGAA+AGG	+	chr2.3:75790432-75790451	MS.gene047317:CDS	45.0%
	CCTTTCTTCGAACCTCTTCT+TGG	-	chr2.3:75790435-75790454	None:intergenic	45.0%
	GGTTCAAAACTGGGAAGAAC+AGG	+	chr2.3:75790453-75790472	MS.gene047317:CDS	45.0%
	TATGCCAACACGTTACACTC+TGG	+	chr2.3:75790334-75790353	MS.gene047317:CDS	45.0%
	TCAAAACTGGGAAGAACAGG+TGG	+	chr2.3:75790456-75790475	MS.gene047317:CDS	45.0%
	TCCAGCTAAGGTGATCAAGA+AGG	+	chr2.3:75790241-75790260	MS.gene047317:CDS	45.0%
	TCCTTCTTGATCACCTTAGC+TGG	-	chr2.3:75790245-75790264	None:intergenic	45.0%
!	AAGACGATCACCGCTTTGTT+TGG	-	chr2.3:75790116-75790135	None:intergenic	45.0%
	ACGGTCATTGCTTGGTTGCA+GGG	+	chr2.3:75790207-75790226	MS.gene047317:CDS	50.0%
	ACGTGCTTACGGTCATTGCT+TGG	+	chr2.3:75790199-75790218	MS.gene047317:CDS	50.0%
	AGGACGTTACGCTGGCAAAA+AGG	+	chr2.3:75790139-75790158	MS.gene047317:CDS	50.0%
	ATGACCGTAAGCACGTTCAC+GGG	-	chr2.3:75790195-75790214	None:intergenic	50.0%
	GTCTTACAAGGACGTTACGC+TGG	+	chr2.3:75790131-75790150	MS.gene047317:CDS	50.0%
	TACGGTCATTGCTTGGTTGC+AGG	+	chr2.3:75790206-75790225	MS.gene047317:CDS	50.0%
!	CTTTGAAGGAGGCCAAGAAG+AGG	+	chr2.3:75790420-75790439	MS.gene047317:CDS	50.0%
!!	GAAGGTCACTGCTTTGAAGG+AGG	+	chr2.3:75790409-75790428	MS.gene047317:CDS	50.0%
	GGAACCCGTGAACGTGCTTA+CGG	+	chr2.3:75790188-75790207	MS.gene047317:CDS	55.0%
	GGTGGTTCTTCACCAAGCTC+AGG	+	chr2.3:75790474-75790493	MS.gene047317:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.3	gene	75790095	75790502	75790095	ID=MS.gene047317
chr2.3	mRNA	75790095	75790502	75790095	ID=MS.gene047317.t1;Parent=MS.gene047317
chr2.3	exon	75790095	75790502	75790095	ID=MS.gene047317.t1.exon1;Parent=MS.gene047317.t1
chr2.3	CDS	75790095	75790502	75790095	ID=cds.MS.gene047317.t1;Parent=MS.gene047317.t1

Gene Sequence

>MS.gene047317

ATGGTGAAATTCTTGAAGCCAAACAAAGCGGTGATCGTCTTACAAGGACGTTACGCTGGCAAAAAGGCTGTCATCGTGAAGAACTTTGATGATGGAACCCGTGAACGTGCTTACGGTCATTGCTTGGTTGCAGGGATCAAGAAGTATCCAGCTAAGGTGATCAAGAAGGATTCAGCTAAGAAAACTGCAAAGAAATCGCGTGTTAAGGCTTTTGTGAAGTTGGTTAACTATCAGCATCTTATGCCAACACGTTACACTCTGGATGTTGATTTGAAGGAGGTTGTTACCACTGATGTTCTTCAGAGTAAGGATAAGAAGGTCACTGCTTTGAAGGAGGCCAAGAAGAGGTTCGAAGAAAGGTTCAAAACTGGGAAGAACAGGTGGTTCTTCACCAAGCTCAGGTTTTGA

Protein sequence

>MS.gene047317.t1

MVKFLKPNKAVIVLQGRYAGKKAVIVKNFDDGTRERAYGHCLVAGIKKYPAKVIKKDSAKKTAKKSRVKAFVKLVNYQHLMPTRYTLDVDLKEVVTTDVLQSKDKKVTALKEAKKRFEERFKTGKNRWFFTKLRF