AlfalfaGEDB | MS.gene073466

M. sativa cultivar XinJiangDaYe / MS.gene073466

KEGG: K02901
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF01777.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene073466.t1	XP_003594893.2	100	135	0	0	1	135	1	135	1.60E-66	261.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene073466.t1	Q05462	89.6	135	14	0	1	135	1	135	6.0e-62	238.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene073466.t1	G7IG15	100.0	135	0	0	1	135	1	135	1.2e-66	261.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050303	MS.gene073466	0.805226	1.48E-49	-1.69E-46
MS.gene052947	MS.gene073466	0.802585	5.23E-49	-1.69E-46
MS.gene055808	MS.gene073466	0.803611	3.21E-49	-1.69E-46
MS.gene056215	MS.gene073466	0.807755	4.33E-50	-1.69E-46
MS.gene06001	MS.gene073466	0.830104	3.54E-55	-1.69E-46
MS.gene060429	MS.gene073466	0.835615	1.51E-56	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene057706	MS.gene073466	PPI
MS.gene005349	MS.gene073466	PPI
MS.gene57462	MS.gene073466	PPI
MS.gene073466	MS.gene57462	PPI
MS.gene073466	MS.gene002051	PPI
MS.gene00620	MS.gene073466	PPI
MS.gene58693	MS.gene073466	PPI
MS.gene073466	MS.gene00618	PPI
MS.gene072057	MS.gene073466	PPI
MS.gene047199	MS.gene073466	PPI
MS.gene047317	MS.gene073466	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene073466.t1	MTR_2g035930	100.000	135	0	0	1	135	1	135	5.67e-95	270
MS.gene073466.t1	MTR_2g006170	99.259	135	1	0	1	135	1	135	1.94e-94	268
MS.gene073466.t1	MTR_3g048580	64.179	67	15	2	70	135	38	96	2.94e-19	77.0

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene073466.t1	AT4G15000	86.667	135	18	1	135	1	135	7.57e-84	241
MS.gene073466.t1	AT3G22230	86.667	135	18	1	135	1	135	1.19e-83	241
MS.gene073466.t1	AT2G32220	80.000	135	27	1	135	1	135	7.46e-78	226
MS.gene073466.t1	AT4G15000	85.366	123	18	1	123	1	123	4.10e-73	214

Find 38 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TACGGCCATTGTCTCGTTGC+AGG	0.261946	2.1:-55154999	MS.gene073466:CDS
CACGATGACGGCTTTCTTCC+CGG	0.266318	2.1:+55155055	None:intergenic
CACTCTTGATGTTGATTTGA+AGG	0.356264	2.1:-55154856	MS.gene073466:CDS
TCCCGGCGTAGCGTCCTTGT+AGG	0.400507	2.1:+55155072	None:intergenic
GGTTTAAGACTGGGAAGAAT+AGG	0.401208	2.1:-55154752	MS.gene073466:CDS
AGGACGATGACGGCTTTGTT+TGG	0.406607	2.1:+55155092	None:intergenic
TACTGATGTTCTTCAGAGTA+AGG	0.417538	2.1:-55154823	MS.gene073466:CDS
TAAGAAGGTGACTGCTTTGA+AGG	0.432710	2.1:-55154799	MS.gene073466:CDS
TCCTTCTTGATAACCTTAGC+TGG	0.439811	2.1:+55154963	None:intergenic
CTTCACACGCGATTTCTTCG+CGG	0.451730	2.1:+55154926	None:intergenic
GGAACCCGTGACCGTGCTTA+CGG	0.460575	2.1:-55155017	MS.gene073466:CDS
GGTGGTTCTTTACCAAGCTC+AGG	0.462687	2.1:-55154731	MS.gene073466:CDS
TCCTACAAGGACGCTACGCC+GGG	0.466145	2.1:-55155073	MS.gene073466:CDS
GTCGAAATTCTTCACGATGA+CGG	0.503904	2.1:+55155043	None:intergenic
TTCGAGGAGAGGTTTAAGAC+TGG	0.506310	2.1:-55154762	MS.gene073466:CDS
ACGGCCATTGTCTCGTTGCA+GGG	0.523979	2.1:-55154998	MS.gene073466:CDS
CGCGAAGAAATCGCGTGTGA+AGG	0.529778	2.1:-55154925	MS.gene073466:CDS
AACGAGTCGGCATCAGATGC+TGG	0.530504	2.1:+55154880	None:intergenic
CGAAGAAGAGGTTCGAGGAG+AGG	0.532977	2.1:-55154773	MS.gene073466:CDS
TCCAGCTAAGGTTATCAAGA+AGG	0.538513	2.1:-55154964	MS.gene073466:CDS
TCGAGGAGAGGTTTAAGACT+GGG	0.540939	2.1:-55154761	MS.gene073466:CDS
AACAGCTCAAAACCTGAGCT+TGG	0.543643	2.1:+55154719	None:intergenic
AAAGCCGTCATCGTCCTACA+AGG	0.551105	2.1:-55155086	MS.gene073466:CDS
AATGGCCGTAAGCACGGTCA+CGG	0.564367	2.1:+55155012	None:intergenic
TCTTCAGAGTAAGGATAAGA+AGG	0.568239	2.1:-55154814	MS.gene073466:CDS
ATGGCCGTAAGCACGGTCAC+GGG	0.578966	2.1:+55155013	None:intergenic
GTCCTACAAGGACGCTACGC+CGG	0.584899	2.1:-55155074	MS.gene073466:CDS
CGAGACAATGGCCGTAAGCA+CGG	0.587108	2.1:+55155006	None:intergenic
TGATCCCTGCAACGAGACAA+TGG	0.587727	2.1:+55154994	None:intergenic
CTTTGAAGGAGGCGAAGAAG+AGG	0.602084	2.1:-55154785	MS.gene073466:CDS
GATCAAGAAGTATCCAGCTA+AGG	0.602579	2.1:-55154976	MS.gene073466:CDS
GGAGGCGAAGAAGAGGTTCG+AGG	0.607573	2.1:-55154778	MS.gene073466:CDS
TTAAGACTGGGAAGAATAGG+TGG	0.614047	2.1:-55154749	MS.gene073466:CDS
GCGTCCTTGTAGGACGATGA+CGG	0.630553	2.1:+55155082	None:intergenic
GAAGGTGACTGCTTTGAAGG+AGG	0.640915	2.1:-55154796	MS.gene073466:CDS
ACATCAAGAGTGTAACGAGT+CGG	0.653533	2.1:+55154867	None:intergenic
TCTTGATGTTGATTTGAAGG+AGG	0.672741	2.1:-55154853	MS.gene073466:CDS
ATCGTGAAGAATTTCGACGA+CGG	0.729202	2.1:-55155038	MS.gene073466:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TACTGATGTTCTTCAGAGTA+AGG	-	chr2.1:55155012-55155031	MS.gene073466:CDS	35.0%
	TCTTCAGAGTAAGGATAAGA+AGG	-	chr2.1:55155021-55155040	MS.gene073466:CDS	35.0%
!!	CACTCTTGATGTTGATTTGA+AGG	-	chr2.1:55154979-55154998	MS.gene073466:CDS	35.0%
!!	TCTTGATGTTGATTTGAAGG+AGG	-	chr2.1:55154982-55155001	MS.gene073466:CDS	35.0%
	ATCGTGAAGAATTTCGACGA+CGG	-	chr2.1:55154797-55154816	MS.gene073466:CDS	40.0%
	GTCGAAATTCTTCACGATGA+CGG	+	chr2.1:55154795-55154814	None:intergenic	40.0%
	TAAGAAGGTGACTGCTTTGA+AGG	-	chr2.1:55155036-55155055	MS.gene073466:CDS	40.0%
	TCCAGCTAAGGTTATCAAGA+AGG	-	chr2.1:55154871-55154890	MS.gene073466:CDS	40.0%
	TTAAGACTGGGAAGAATAGG+TGG	-	chr2.1:55155086-55155105	MS.gene073466:CDS	40.0%
!	ACATCAAGAGTGTAACGAGT+CGG	+	chr2.1:55154971-55154990	None:intergenic	40.0%
!	GGTTTAAGACTGGGAAGAAT+AGG	-	chr2.1:55155083-55155102	MS.gene073466:CDS	40.0%
!	TCCTTCTTGATAACCTTAGC+TGG	+	chr2.1:55154875-55154894	None:intergenic	40.0%
!!	GATCAAGAAGTATCCAGCTA+AGG	-	chr2.1:55154859-55154878	MS.gene073466:CDS	40.0%
!	TCGAGGAGAGGTTTAAGACT+GGG	-	chr2.1:55155074-55155093	MS.gene073466:CDS	45.0%
!	TTCGAGGAGAGGTTTAAGAC+TGG	-	chr2.1:55155073-55155092	MS.gene073466:CDS	45.0%
	AAAGCCGTCATCGTCCTACA+AGG	-	chr2.1:55154749-55154768	MS.gene073466:CDS	50.0%
	CTTCACACGCGATTTCTTCG+CGG	+	chr2.1:55154912-55154931	None:intergenic	50.0%
	GGTGGTTCTTTACCAAGCTC+AGG	-	chr2.1:55155104-55155123	MS.gene073466:CDS	50.0%
	TGATCCCTGCAACGAGACAA+TGG	+	chr2.1:55154844-55154863	None:intergenic	50.0%
!	CTTTGAAGGAGGCGAAGAAG+AGG	-	chr2.1:55155050-55155069	MS.gene073466:CDS	50.0%
!!	AGGACGATGACGGCTTTGTT+TGG	+	chr2.1:55154746-55154765	None:intergenic	50.0%
!!	GAAGGTGACTGCTTTGAAGG+AGG	-	chr2.1:55155039-55155058	MS.gene073466:CDS	50.0%
	AACGAGTCGGCATCAGATGC+TGG	+	chr2.1:55154958-55154977	None:intergenic	55.0%
	AATGGCCGTAAGCACGGTCA+CGG	+	chr2.1:55154826-55154845	None:intergenic	55.0%
	ACGGCCATTGTCTCGTTGCA+GGG	-	chr2.1:55154837-55154856	MS.gene073466:CDS	55.0%
	CGAAGAAGAGGTTCGAGGAG+AGG	-	chr2.1:55155062-55155081	MS.gene073466:CDS	55.0%
	CGAGACAATGGCCGTAAGCA+CGG	+	chr2.1:55154832-55154851	None:intergenic	55.0%
	CGCGAAGAAATCGCGTGTGA+AGG	-	chr2.1:55154910-55154929	MS.gene073466:CDS	55.0%
	GCGTCCTTGTAGGACGATGA+CGG	+	chr2.1:55154756-55154775	None:intergenic	55.0%
	TACGGCCATTGTCTCGTTGC+AGG	-	chr2.1:55154836-55154855	MS.gene073466:CDS	55.0%
!	CACGATGACGGCTTTCTTCC+CGG	+	chr2.1:55154783-55154802	None:intergenic	55.0%
	ATGGCCGTAAGCACGGTCAC+GGG	+	chr2.1:55154825-55154844	None:intergenic	60.0%
	GGAACCCGTGACCGTGCTTA+CGG	-	chr2.1:55154818-55154837	MS.gene073466:CDS	60.0%
	GGAGGCGAAGAAGAGGTTCG+AGG	-	chr2.1:55155057-55155076	MS.gene073466:CDS	60.0%
	GTCCTACAAGGACGCTACGC+CGG	-	chr2.1:55154761-55154780	MS.gene073466:CDS	60.0%
	TCCTACAAGGACGCTACGCC+GGG	-	chr2.1:55154762-55154781	MS.gene073466:CDS	60.0%
	TCCCGGCGTAGCGTCCTTGT+AGG	+	chr2.1:55154766-55154785	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.1	gene	55154725	55155132	55154725	ID=MS.gene073466
chr2.1	mRNA	55154725	55155132	55154725	ID=MS.gene073466.t1;Parent=MS.gene073466
chr2.1	exon	55154725	55155132	55154725	ID=MS.gene073466.t1.exon1;Parent=MS.gene073466.t1
chr2.1	CDS	55154725	55155132	55154725	ID=cds.MS.gene073466.t1;Parent=MS.gene073466.t1

Gene Sequence

>MS.gene073466

ATGGTGAAATTCTTGAAGCCAAACAAAGCCGTCATCGTCCTACAAGGACGCTACGCCGGGAAGAAAGCCGTCATCGTGAAGAATTTCGACGACGGAACCCGTGACCGTGCTTACGGCCATTGTCTCGTTGCAGGGATCAAGAAGTATCCAGCTAAGGTTATCAAGAAGGATTCAGCGAAGAAAACCGCGAAGAAATCGCGTGTGAAGGCTTTCGTGAAGCTTGTTAACTACCAGCATCTGATGCCGACTCGTTACACTCTTGATGTTGATTTGAAGGAGGTTGTTACTACTGATGTTCTTCAGAGTAAGGATAAGAAGGTGACTGCTTTGAAGGAGGCGAAGAAGAGGTTCGAGGAGAGGTTTAAGACTGGGAAGAATAGGTGGTTCTTTACCAAGCTCAGGTTTTGA

Protein sequence

>MS.gene073466.t1

MVKFLKPNKAVIVLQGRYAGKKAVIVKNFDDGTRDRAYGHCLVAGIKKYPAKVIKKDSAKKTAKKSRVKAFVKLVNYQHLMPTRYTLDVDLKEVVTTDVLQSKDKKVTALKEAKKRFEERFKTGKNRWFFTKLRF