Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene008847.t1 | XP_013446173.1 | 98.7 | 452 | 6 | 0 | 63 | 514 | 59 | 510 | 6.40E-249 | 869.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene008847.t1 | Q9LPV5 | 75.5 | 432 | 106 | 0 | 64 | 495 | 62 | 493 | 3.2e-188 | 659.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene008847.t1 | A0A072U314 | 98.7 | 452 | 6 | 0 | 63 | 514 | 59 | 510 | 4.6e-249 | 869.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene08733 | MS.gene008847 | PPI |
| MS.gene007704 | MS.gene008847 | PPI |
| MS.gene08732 | MS.gene008847 | PPI |
| MS.gene050095 | MS.gene008847 | PPI |
| MS.gene007705 | MS.gene008847 | PPI |
| MS.gene08666 | MS.gene008847 | PPI |
| MS.gene050096 | MS.gene008847 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene008847.t1 | MTR_8g069775 | 98.673 | 452 | 6 | 0 | 63 | 514 | 59 | 510 | 0.0 | 904 |
| MS.gene008847.t1 | MTR_4g057890 | 60.667 | 450 | 175 | 2 | 63 | 512 | 79 | 526 | 0.0 | 580 |
| MS.gene008847.t1 | MTR_4g057865 | 60.444 | 450 | 176 | 2 | 63 | 512 | 79 | 526 | 0.0 | 579 |
| MS.gene008847.t1 | MTR_8g069770 | 96.875 | 64 | 2 | 0 | 1 | 64 | 1 | 64 | 1.17e-41 | 143 |
| MS.gene008847.t1 | MTR_7g097110 | 88.333 | 60 | 7 | 0 | 1 | 60 | 1 | 60 | 5.11e-33 | 120 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene008847.t1 | AT1G12940 | 75.289 | 433 | 107 | 0 | 63 | 495 | 61 | 493 | 0.0 | 685 |
| MS.gene008847.t1 | AT5G60770 | 53.728 | 523 | 208 | 8 | 12 | 514 | 19 | 527 | 0.0 | 562 |
| MS.gene008847.t1 | AT1G08090 | 57.489 | 454 | 189 | 2 | 63 | 514 | 79 | 530 | 0.0 | 557 |
| MS.gene008847.t1 | AT1G08100 | 57.534 | 438 | 185 | 1 | 63 | 499 | 77 | 514 | 0.0 | 538 |
| MS.gene008847.t1 | AT3G45060 | 52.418 | 517 | 227 | 5 | 15 | 514 | 28 | 542 | 0.0 | 526 |
| MS.gene008847.t1 | AT5G60780 | 56.828 | 454 | 194 | 1 | 63 | 514 | 86 | 539 | 0.0 | 523 |
| MS.gene008847.t1 | AT5G14570 | 50.682 | 440 | 195 | 3 | 63 | 485 | 56 | 490 | 1.65e-149 | 437 |
| MS.gene008847.t1 | AT2G20490 | 79.688 | 64 | 13 | 0 | 1 | 64 | 1 | 64 | 2.12e-31 | 115 |
| MS.gene008847.t1 | AT2G20490 | 79.688 | 64 | 13 | 0 | 1 | 64 | 1 | 64 | 2.12e-31 | 115 |
| MS.gene008847.t1 | AT2G20490 | 78.125 | 64 | 13 | 1 | 1 | 64 | 1 | 63 | 4.82e-29 | 109 |
Find 96 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTGGTGTTGCATCTGTTTC+TGG | 0.074073 | 8.1:-35160577 | MS.gene008847:CDS |
| TTCCAGCAGTGTGAAGTTTA+AGG | 0.193334 | 8.1:+35159267 | None:intergenic |
| TGGTTTATTATCTGATTTAA+TGG | 0.218179 | 8.1:-35159195 | MS.gene008847:CDS |
| GCTGCTTGTGGAATGACTTT+TGG | 0.252766 | 8.1:-35159029 | MS.gene008847:CDS |
| TTGGAATGAGGGGTAGATTA+TGG | 0.258780 | 8.1:-35159163 | MS.gene008847:CDS |
| GATTTAATGGCTAAGAGATT+TGG | 0.279825 | 8.1:-35159182 | MS.gene008847:CDS |
| TTGACTTATGGTTATTGTTT+TGG | 0.287818 | 8.1:-35159344 | MS.gene008847:CDS |
| GGAATCATTGCTGCAAGTTT+TGG | 0.302049 | 8.1:-35159248 | MS.gene008847:CDS |
| GGTGGGTGGGGTAACCTTGG+TGG | 0.319262 | 8.1:-35159626 | MS.gene008847:CDS |
| TATCATAGTTTGAAGAAATC+AGG | 0.346076 | 8.1:-35159440 | MS.gene008847:CDS |
| GGAAAATAGATTAAGAATAA+TGG | 0.358502 | 8.1:+35157892 | None:intergenic |
| TTAAGGTTGTCACGGATTAT+AGG | 0.361381 | 8.1:+35160625 | None:intergenic |
| AGAAAGAGGAATAACTCTAA+TGG | 0.367970 | 8.1:-35157941 | MS.gene008847:CDS |
| AATGGGAACTGCTTGTGATT+TGG | 0.383228 | 8.1:-35160533 | MS.gene008847:CDS |
| AACCTTAAACTTCACACTGC+TGG | 0.383716 | 8.1:-35159269 | MS.gene008847:CDS |
| CAGTGTTACATAAATGATAA+TGG | 0.389907 | 8.1:-35169526 | MS.gene008847:CDS |
| ATCCTTTGAAGAAAATTAGT+TGG | 0.392329 | 8.1:+35157977 | None:intergenic |
| TATCCGTTGCTGTTAGGTTA+AGG | 0.394367 | 8.1:+35160608 | None:intergenic |
| AACCTAACAGCAACGGATAT+AGG | 0.398418 | 8.1:-35160604 | MS.gene008847:CDS |
| GAAAGAGGAATAACTCTAAT+GGG | 0.408051 | 8.1:-35157940 | MS.gene008847:CDS |
| CATGGTGTGTTAAATTATAG+AGG | 0.409317 | 8.1:-35159380 | MS.gene008847:CDS |
| GAAGGAATCACCACTGGGTT+CGG | 0.421070 | 8.1:-35168338 | MS.gene008847:CDS |
| TTCCTATATCCGTTGCTGTT+AGG | 0.424382 | 8.1:+35160602 | None:intergenic |
| CACAGAAGAAGATTACTATA+TGG | 0.427355 | 8.1:-35157827 | MS.gene008847:CDS |
| CTTCTTGTGAGGTTCTTCAC+TGG | 0.428270 | 8.1:-35160421 | MS.gene008847:CDS |
| TCAGGTGGGTGGGGTAACCT+TGG | 0.436260 | 8.1:-35159629 | MS.gene008847:intron |
| ACTGTTGTTGGAAGAGCAAA+TGG | 0.438974 | 8.1:-35160340 | MS.gene008847:CDS |
| GGAAGAGCAAATGGTTTCTC+AGG | 0.441053 | 8.1:-35160331 | MS.gene008847:intron |
| TCTATCATCAATTCTCCTAC+TGG | 0.442898 | 8.1:-35160448 | MS.gene008847:CDS |
| GGGTGGGGTAACCTTGGTGG+AGG | 0.446755 | 8.1:-35159623 | MS.gene008847:CDS |
| TCCTTTGAAGAAAATTAGTT+GGG | 0.459131 | 8.1:+35157978 | None:intergenic |
| AGGTCCTTAGGAGTCATATC+AGG | 0.460197 | 8.1:-35158039 | MS.gene008847:CDS |
| GGCTAAGAGATTTGGAATGA+GGG | 0.467670 | 8.1:-35159174 | MS.gene008847:CDS |
| TGGATTTGTCAAACGTTGGC+TGG | 0.472815 | 8.1:-35159134 | MS.gene008847:CDS |
| ATTGGTTTCTCAGGTGGGTG+GGG | 0.473443 | 8.1:-35159638 | MS.gene008847:intron |
| GGGGTAGATTATGGTGTTTG+TGG | 0.475044 | 8.1:-35159154 | MS.gene008847:CDS |
| AATTGGTTTCTCAGGTGGGT+GGG | 0.480458 | 8.1:-35159639 | MS.gene008847:intron |
| TGGCTAAGAGATTTGGAATG+AGG | 0.480994 | 8.1:-35159175 | MS.gene008847:CDS |
| GTGCGTCTGAAGAAGCGCTT+TGG | 0.482078 | 8.1:-35167322 | MS.gene008847:CDS |
| TGCAACAAATTCAGGTCCTT+AGG | 0.486150 | 8.1:-35158051 | MS.gene008847:intron |
| TTCCGAGGTGGCTGCTGAGT+TGG | 0.491045 | 8.1:+35167292 | None:intergenic |
| AAACTATGATAATTACCATC+TGG | 0.492765 | 8.1:+35159452 | None:intergenic |
| ATGATAAATTCTCAGACCAT+AGG | 0.495274 | 8.1:-35167345 | MS.gene008847:CDS |
| ACATACCTTCTTGAGACAAA+AGG | 0.496721 | 8.1:+35158999 | None:intergenic |
| TTCAGCTATGATATTGTCAA+TGG | 0.501509 | 8.1:+35159310 | None:intergenic |
| GATAATAGTAGAAGTGAAAG+AGG | 0.507879 | 8.1:-35157745 | MS.gene008847:CDS |
| AAGATTACTATATGGCTGAA+TGG | 0.518793 | 8.1:-35157819 | MS.gene008847:CDS |
| GTTCAACAAAATTCACTGCA+TGG | 0.520686 | 8.1:-35159550 | MS.gene008847:CDS |
| AACTCAAAGGAGAGGGAGAA+AGG | 0.520853 | 8.1:-35157796 | MS.gene008847:CDS |
| TAAGGTGCATATTATGATCA+TGG | 0.523894 | 8.1:+35157915 | None:intergenic |
| CATTCCTGATATGACTCCTA+AGG | 0.529469 | 8.1:+35158035 | None:intergenic |
| CATTTCAACTAGGCCTTCTG+AGG | 0.536507 | 8.1:-35157710 | MS.gene008847:CDS |
| TCAGATAATAAACCACCTCC+TGG | 0.536893 | 8.1:+35159203 | None:intergenic |
| CTGTTAGGTTAAGGTTGTCA+CGG | 0.537447 | 8.1:+35160617 | None:intergenic |
| GCTTCTTCAGACGCACCCTA+TGG | 0.540494 | 8.1:+35167329 | None:intergenic |
| AAATTGGTTTCTCAGGTGGG+TGG | 0.540925 | 8.1:-35159640 | MS.gene008847:intron |
| GGAGTCATATCAGGAATGAC+AGG | 0.542118 | 8.1:-35158030 | MS.gene008847:CDS |
| GTGTGTTAAATTATAGAGGG+TGG | 0.543363 | 8.1:-35159376 | MS.gene008847:CDS |
| TTTGGTCAAGATTTACCAGA+TGG | 0.543573 | 8.1:-35159467 | MS.gene008847:CDS |
| TCAGGAATGACAGGAGGAGG+AGG | 0.547520 | 8.1:-35158021 | MS.gene008847:CDS |
| CTGAATGGAACTCAAAGGAG+AGG | 0.547602 | 8.1:-35157804 | MS.gene008847:CDS |
| GGAACTGCTTGTGATTTGGT+TGG | 0.550581 | 8.1:-35160529 | MS.gene008847:CDS |
| TGGTCTGAGAATTTATCATC+GGG | 0.555795 | 8.1:+35167349 | None:intergenic |
| ATAGATTAAGAATAATGGTA+AGG | 0.558412 | 8.1:+35157897 | None:intergenic |
| GCAACGGATATAGGAAATGC+TGG | 0.559363 | 8.1:-35160595 | MS.gene008847:CDS |
| TGATAAATTCTCAGACCATA+GGG | 0.566431 | 8.1:-35167344 | MS.gene008847:CDS |
| ATGGTCTGAGAATTTATCAT+CGG | 0.567138 | 8.1:+35167348 | None:intergenic |
| ATATCAGGAATGACAGGAGG+AGG | 0.569761 | 8.1:-35158024 | MS.gene008847:CDS |
| GAAAATTGAACATTTCAACT+AGG | 0.570170 | 8.1:-35157720 | MS.gene008847:CDS |
| TGCCAACTCAGCAGCCACCT+CGG | 0.572303 | 8.1:-35167294 | MS.gene008847:CDS |
| AAACTGAGTAGAAACAAAAG+TGG | 0.576179 | 8.1:+35160387 | None:intergenic |
| AGGGAGAAAGGCTCTCATCA+TGG | 0.577211 | 8.1:-35157784 | MS.gene008847:CDS |
| ATAGAAGTGAAGTAAACAGC+AGG | 0.583371 | 8.1:+35160466 | None:intergenic |
| CTCTATAATTTAACACACCA+TGG | 0.586174 | 8.1:+35159381 | None:intergenic |
| ATTTCAAAAGTAATTTACCA+TGG | 0.589099 | 8.1:-35159398 | MS.gene008847:CDS |
| TGTATAAGTGAAAATACAAG+TGG | 0.589679 | 8.1:+35159581 | None:intergenic |
| TTTGTGGATTTGTCAAACGT+TGG | 0.593191 | 8.1:-35159138 | MS.gene008847:CDS |
| GCTAAGAGATTTGGAATGAG+GGG | 0.599275 | 8.1:-35159173 | MS.gene008847:CDS |
| AATAGTAGAAGTGAAAGAGG+TGG | 0.601395 | 8.1:-35157742 | MS.gene008847:CDS |
| ACAGGAGGAGGAGGAAACGT+TGG | 0.608583 | 8.1:-35158012 | MS.gene008847:CDS |
| TGAATGGAACTCAAAGGAGA+GGG | 0.609630 | 8.1:-35157803 | MS.gene008847:CDS |
| TTTCAGAAGGAATCACCACT+GGG | 0.620010 | 8.1:-35168343 | MS.gene008847:intron |
| TATGGCTGAATGGAACTCAA+AGG | 0.622357 | 8.1:-35157809 | MS.gene008847:CDS |
| ATGGTGTGTTAAATTATAGA+GGG | 0.629408 | 8.1:-35159379 | MS.gene008847:CDS |
| TCACGGATTATAGGAACAAG+TGG | 0.646242 | 8.1:+35160634 | None:intergenic |
| GTCATATCAGGAATGACAGG+AGG | 0.646603 | 8.1:-35158027 | MS.gene008847:CDS |
| CAACCTTAACCTAACAGCAA+CGG | 0.647550 | 8.1:-35160611 | MS.gene008847:CDS |
| GGTGGTGTAACATCCTCAGA+AGG | 0.651845 | 8.1:+35157697 | None:intergenic |
| GACTCTGTTGCCGAACCCAG+TGG | 0.655330 | 8.1:+35168328 | None:intergenic |
| CTATCTCAGTACTTCCGAGG+TGG | 0.658485 | 8.1:+35167280 | None:intergenic |
| GCAACAGAGTCAGCTCACCC+AGG | 0.664796 | 8.1:-35168316 | MS.gene008847:intron |
| TCACTATCTCAGTACTTCCG+AGG | 0.676583 | 8.1:+35167277 | None:intergenic |
| AAAGCAGCAGAAGCAAGACG+AGG | 0.678632 | 8.1:+35160505 | None:intergenic |
| TGACAAAGTTTACACTACCA+AGG | 0.686778 | 8.1:-35169503 | MS.gene008847:intron |
| GTTGTGTTGCTCCTCCACCA+AGG | 0.696287 | 8.1:+35159612 | None:intergenic |
| CTCACAAGAAGAAAACCAGT+AGG | 0.723236 | 8.1:+35160433 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 35157684 | 35169557 | 35157684 | ID=MS.gene008847 |
| chr8.1 | mRNA | 35157684 | 35169557 | 35157684 | ID=MS.gene008847.t1;Parent=MS.gene008847 |
| chr8.1 | exon | 35169504 | 35169557 | 35169504 | ID=MS.gene008847.t1.exon1;Parent=MS.gene008847.t1 |
| chr8.1 | CDS | 35169504 | 35169557 | 35169504 | ID=cds.MS.gene008847.t1;Parent=MS.gene008847.t1 |
| chr8.1 | exon | 35168317 | 35168359 | 35168317 | ID=MS.gene008847.t1.exon2;Parent=MS.gene008847.t1 |
| chr8.1 | CDS | 35168317 | 35168359 | 35168317 | ID=cds.MS.gene008847.t1;Parent=MS.gene008847.t1 |
| chr8.1 | exon | 35167292 | 35167382 | 35167292 | ID=MS.gene008847.t1.exon3;Parent=MS.gene008847.t1 |
| chr8.1 | CDS | 35167292 | 35167382 | 35167292 | ID=cds.MS.gene008847.t1;Parent=MS.gene008847.t1 |
| chr8.1 | exon | 35160332 | 35160669 | 35160332 | ID=MS.gene008847.t1.exon4;Parent=MS.gene008847.t1 |
| chr8.1 | CDS | 35160332 | 35160669 | 35160332 | ID=cds.MS.gene008847.t1;Parent=MS.gene008847.t1 |
| chr8.1 | exon | 35159005 | 35159647 | 35159005 | ID=MS.gene008847.t1.exon5;Parent=MS.gene008847.t1 |
| chr8.1 | CDS | 35159005 | 35159647 | 35159005 | ID=cds.MS.gene008847.t1;Parent=MS.gene008847.t1 |
| chr8.1 | exon | 35157684 | 35158059 | 35157684 | ID=MS.gene008847.t1.exon6;Parent=MS.gene008847.t1 |
| chr8.1 | CDS | 35157684 | 35158059 | 35157684 | ID=cds.MS.gene008847.t1;Parent=MS.gene008847.t1 |
| Gene Sequence |
| Protein sequence |
