AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene007704

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene007704.t1 MTR_4g057890 99.430 526 3 0 1 526 1 526 0.0 1071
MS.gene007704.t1 MTR_4g057865 99.240 526 4 0 1 526 1 526 0.0 1070
MS.gene007704.t1 MTR_8g069775 61.554 489 186 2 38 524 18 506 0.0 591
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene007704.t1 AT1G08090 79.017 529 108 1 1 526 1 529 0.0 882
MS.gene007704.t1 AT5G60770 79.017 529 105 2 1 526 1 526 0.0 877
MS.gene007704.t1 AT1G08100 75.048 517 126 2 7 521 6 521 0.0 820
MS.gene007704.t1 AT5G60780 72.795 533 137 3 2 526 6 538 0.0 791
MS.gene007704.t1 AT3G45060 72.285 534 135 4 5 526 9 541 0.0 782
MS.gene007704.t1 AT1G12940 59.259 486 194 2 38 522 20 502 0.0 605
MS.gene007704.t1 AT5G14570 48.723 470 219 4 41 493 18 482 5.56e-152 444

Find 138 sgRNAs with CRISPR-Local

Find 164 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCATGTCATCATGGGTATTT+TGG 0.159771 4.3:+43150989 MS.gene007704:CDS
GCCGGAGTCGCTTCGGTTTC+AGG 0.162646 4.3:+43150594 MS.gene007704:CDS
TGCTGCTGGTTGGGGTAATA+TGG 0.200878 4.3:+43150857 MS.gene007704:CDS
ATCAACTGGTAATGCAAATT+TGG 0.211102 4.3:-43150397 None:intergenic
CCTGCTTTATGGTTACTCTA+TGG 0.215654 4.3:+43151233 MS.gene007704:CDS
ATGTTTAATAGTAAAATTAT+TGG 0.217642 4.3:+43150819 MS.gene007704:CDS
AAAATTATTGGACTTGTAAA+TGG 0.231743 4.3:+43150831 MS.gene007704:CDS
CATTTGTTTCATGCCAATAT+TGG 0.240129 4.3:+43150787 MS.gene007704:CDS
GGAATCATTGCTGCCTCCTT+TGG 0.289222 4.3:+43151544 MS.gene007704:CDS
CAACAGCTTCAGGTCTTTCA+TGG 0.291908 4.3:+43151902 MS.gene007704:CDS
CGTCGGATTTCGTGAGGTTA+AGG 0.293043 4.3:-43150563 None:intergenic
CACTCAGCTTATTATGCCTT+TGG 0.295935 4.3:+43150890 MS.gene007704:CDS
GTTTATGATTGGTTTCTCTT+TGG 0.307610 4.3:+43150761 MS.gene007704:CDS
AGCTCCATGCATGGAGTAAC+TGG 0.308320 4.3:+43150315 MS.gene007704:CDS
AAATGGTACTGCTGCTGGTT+GGG 0.317688 4.3:+43150848 MS.gene007704:CDS
GATGTTGCAGCAAGATTATT+CGG 0.318773 4.3:+43151610 MS.gene007704:CDS
GGAATAATATCAGGTTTAAC+TGG 0.326492 4.3:+43151817 MS.gene007704:CDS
GGTTGCATCAGCCAAGTCTT+AGG 0.340353 4.3:+43152070 MS.gene007704:CDS
TGAAAAGAGTTTGAAAACTT+TGG 0.345000 4.3:-43150433 None:intergenic
TTCATATCAAGAAGATCTTT+AGG 0.352927 4.3:+43151796 MS.gene007704:CDS
GGTGCAATTTGTGACCTATT+AGG 0.354243 4.3:+43150642 MS.gene007704:CDS
CGCCATTAGTCCCGATCATC+CGG 0.360837 4.3:+43150535 MS.gene007704:CDS
ACATAGTACTCATCCAATAT+TGG 0.362676 4.3:-43150800 None:intergenic
AGAAAGATATCCATGATAAA+TGG 0.374158 4.3:-43150482 None:intergenic
TTCCGGCTGTGTGTAGCTTT+AGG 0.375698 4.3:-43151525 None:intergenic
AGATCTTTAGGAATAATATC+AGG 0.377046 4.3:+43151808 MS.gene007704:CDS
GTGCTACTCCTTTCACTGCT+TGG 0.383160 4.3:+43150937 MS.gene007704:CDS
GGTATGCTATAACAAATTAT+AGG 0.383668 4.3:+43151196 MS.gene007704:CDS
CTGCTGGTTATATAGCAGTT+AGG 0.391168 4.3:+43150739 MS.gene007704:CDS
TAGTGGGTAGGAGTTGTATT+TGG 0.393817 4.3:-43152150 None:intergenic
ACTCCATGCATGGAGCTTCC+AGG 0.398090 4.3:-43150309 None:intergenic
GGTTGGGGTAATATGGGTGG+TGG 0.398747 4.3:+43150864 MS.gene007704:CDS
GCAGGTGGAAACTTTGGTTC+CGG 0.404321 4.3:+43151841 MS.gene007704:CDS
AACCATAACGTGGACCTAAT+AGG 0.411808 4.3:-43150656 None:intergenic
CTGCTTTATGGTTACTCTAT+GGG 0.414595 4.3:+43151234 MS.gene007704:CDS
GCTGCTGGTTGGGGTAATAT+GGG 0.417787 4.3:+43150858 MS.gene007704:CDS
GCTGCATGTGGTGCAACATT+TGG 0.420104 4.3:+43151763 MS.gene007704:CDS
CCTTGGTGCTTTGCAGAAGA+AGG 0.426831 4.3:+43151046 MS.gene007704:CDS
AACATAGTTGCTCGTCCATT+CGG 0.426966 4.3:+43151574 MS.gene007704:CDS
GTTGGGGTAATATGGGTGGT+GGG 0.435234 4.3:+43150865 MS.gene007704:CDS
ATAGCAGTTAGGTTTATGAT+TGG 0.436014 4.3:+43150750 MS.gene007704:CDS
TGGATGGGTGCAATGATTGT+TGG 0.437054 4.3:+43151922 MS.gene007704:CDS
AAAGATCTTCTTGATATGAA+TGG 0.442192 4.3:-43151793 None:intergenic
TATATCAATGGCTGATATTG+AGG 0.442270 4.3:+43150281 None:intergenic
AAATATCAACTGTGTTAATC+CGG 0.442980 4.3:-43151860 None:intergenic
GAGGAAGACTATGGACACTA+TGG 0.445715 4.3:+43151638 MS.gene007704:CDS
GACCTATTAGGTCCACGTTA+TGG 0.448846 4.3:+43150654 MS.gene007704:CDS
ACTGGTGCAGGTGGAAACTT+TGG 0.451991 4.3:+43151835 MS.gene007704:CDS
TCGGCATGAGAGGAAGACTA+TGG 0.452862 4.3:+43151629 MS.gene007704:CDS
TGGTTGGTTGCATGTCATCA+TGG 0.455374 4.3:+43150980 MS.gene007704:CDS
GGAAAATGAACAAATGCAAC+AGG 0.456403 4.3:-43151955 None:intergenic
TGAGAACCTTCCATTTATCA+TGG 0.456413 4.3:+43150472 MS.gene007704:CDS
TTGGTGCAGTTAGCATAATA+AGG 0.458274 4.3:-43150686 None:intergenic
GCTGATATTGAGGGTTCACC+TGG 0.463825 4.3:+43150291 MS.gene007704:CDS
AGCTTCAGGTCTTTCATGGA+TGG 0.465615 4.3:+43151906 MS.gene007704:CDS
AGAAAACTCCTCCTAGGGTT+TGG 0.467221 4.3:-43151666 None:intergenic
TAAGGTTGTCCCGGATGATC+GGG 0.469055 4.3:-43150545 None:intergenic
ATATGCAGAAAACTCCTCCT+AGG 0.470665 4.3:-43151672 None:intergenic
AACACTATTACACTTCTGAA+TGG 0.470788 4.3:+43152025 MS.gene007704:CDS
TGGTAACGCCGGAGTCGCTT+CGG 0.471102 4.3:+43150587 MS.gene007704:CDS
GGTTACCATCAGGTAAATCT+TGG 0.480850 4.3:-43151025 None:intergenic
TCCCGGATGATCGGGACTAA+TGG 0.482655 4.3:-43150537 None:intergenic
CTTGTAAATGGTACTGCTGC+TGG 0.488890 4.3:+43150843 MS.gene007704:CDS
AATGGTACTGCTGCTGGTTG+GGG 0.493227 4.3:+43150849 MS.gene007704:CDS
AAAGGAGGCAGCAATGATTC+CGG 0.493669 4.3:-43151542 None:intergenic
ATATCAGGTTTAACTGGTGC+AGG 0.496822 4.3:+43151823 MS.gene007704:CDS
AAACTCCTCCTAGGGTTTGG+AGG 0.497227 4.3:-43151663 None:intergenic
TTGCTGAGTATTTCTATGAC+AGG 0.498846 4.3:+43151283 MS.gene007704:CDS
TAAATGGTACTGCTGCTGGT+TGG 0.504033 4.3:+43150847 MS.gene007704:CDS
TCTTCCAGTTACTCCATGCA+TGG 0.504862 4.3:-43150319 None:intergenic
TTGGGGTAATATGGGTGGTG+GGG 0.507796 4.3:+43150866 MS.gene007704:CDS
CCATAGAGTAACCATAAAGC+AGG 0.508140 4.3:-43151233 None:intergenic
TCGTGAGGTTAAGGTTGTCC+CGG 0.513783 4.3:-43150554 None:intergenic
TTAAGGTTGTCCCGGATGAT+CGG 0.515983 4.3:-43150546 None:intergenic
ATGTTTCGTCTCGACATTCG+CGG 0.524370 4.3:+43150509 MS.gene007704:CDS
CTATAACAAATTATAGGACA+TGG 0.524591 4.3:+43151202 MS.gene007704:CDS
GTGGTATCAGTTGGAACCAT+TGG 0.524676 4.3:-43150372 None:intergenic
GATTTGTGTTCAGAATCAAC+TGG 0.525048 4.3:-43150411 None:intergenic
ATGAAGATGAAGTACAGAAA+GGG 0.525551 4.3:+43152049 MS.gene007704:CDS
AATGAAGATGAAGTACAGAA+AGG 0.527504 4.3:+43152048 MS.gene007704:CDS
TCATTTGTTGAAAATGCTGC+TGG 0.536611 4.3:+43150723 MS.gene007704:CDS
CATTGCTGCCTCCTTTGGAA+TGG 0.536934 4.3:+43151549 MS.gene007704:CDS
GATTTACCTGATGGTAACCT+TGG 0.538623 4.3:+43151029 MS.gene007704:CDS
TCCATGATCCTCTTCTCAGT+TGG 0.543814 4.3:+43151733 MS.gene007704:CDS
ATATCAATGGCTGATATTGA+GGG 0.546989 4.3:+43150282 None:intergenic
GTCATCAGTTGCTTCACCAA+TGG 0.548350 4.3:+43150356 MS.gene007704:CDS
ATGATGACATGCAACCAACC+AGG 0.550646 4.3:-43150978 None:intergenic
GCTTCAGGTCTTTCATGGAT+GGG 0.552112 4.3:+43151907 MS.gene007704:CDS
GGTTGGTTGCATGTCATCAT+GGG 0.555568 4.3:+43150981 MS.gene007704:CDS
AACCTAAAGCTACACACAGC+CGG 0.558064 4.3:+43151523 MS.gene007704:CDS
CTTGTGCTCCAACTGAGAAG+AGG 0.561854 4.3:-43151741 None:intergenic
GGCATATAACTTGTACTAGT+GGG 0.562820 4.3:-43152166 None:intergenic
TGGGTAGGAGTTGTATTTGG+TGG 0.563220 4.3:-43152147 None:intergenic
ACCTGAAACCGAAGCGACTC+CGG 0.567599 4.3:-43150595 None:intergenic
AACTATGTTTGCCATTCCAA+AGG 0.572275 4.3:-43151560 None:intergenic
CTTGGTGCTTTGCAGAAGAA+GGG 0.574830 4.3:+43151047 MS.gene007704:CDS
TCACCTGGAAGCTCCATGCA+TGG 0.576261 4.3:+43150306 MS.gene007704:CDS
AATTTGGCTGTGGTATCAGT+TGG 0.576596 4.3:-43150381 None:intergenic
GCAATTCTCCAAGCAGTGAA+AGG 0.578221 4.3:-43150945 None:intergenic
AATGACATACAAAACACTGT+TGG 0.582355 4.3:-43150705 None:intergenic
TTCTCAGCAAACCTAAGACT+TGG 0.583555 4.3:-43152081 None:intergenic
AAATGGAAGGTTCTCATGTG+AGG 0.586590 4.3:-43150465 None:intergenic
AAAGCACCAAGGTTACCATC+AGG 0.588442 4.3:-43151035 None:intergenic
TATGAGTTGATTAGAAGAGC+TGG 0.589543 4.3:+43150915 MS.gene007704:CDS
GAGAATAGTCGTTCTGAGAG+AGG 0.592972 4.3:+43152099 MS.gene007704:CDS
CTATGGATCCTCCAAACCCT+AGG 0.594763 4.3:+43151655 MS.gene007704:CDS
TATGTTTGCCATTCCAAAGG+AGG 0.596917 4.3:-43151557 None:intergenic
TTAGGCCAAGATTTACCTGA+TGG 0.597509 4.3:+43151020 MS.gene007704:CDS
GCAAGATTATTCGGCATGAG+AGG 0.600610 4.3:+43151619 MS.gene007704:CDS
TCTGATGCATATCCGCCGAA+TGG 0.605405 4.3:-43151589 None:intergenic
TACCAACGTCGGATTTCGTG+AGG 0.605986 4.3:-43150569 None:intergenic
TCAGGTTTAACTGGTGCAGG+TGG 0.608725 4.3:+43151826 MS.gene007704:CDS
ATCATGGATAGTACTGCGAT+CGG 0.609698 4.3:-43151718 None:intergenic
TGGATCCTCCAAACCCTAGG+AGG 0.611729 4.3:+43151658 MS.gene007704:CDS
ATAGTTGCTCGTCCATTCGG+CGG 0.613192 4.3:+43151577 MS.gene007704:CDS
CCTTCTTCTGCAAAGCACCA+AGG 0.613924 4.3:-43151046 None:intergenic
TATAACTTGTACTAGTGGGT+AGG 0.614728 4.3:-43152162 None:intergenic
TCCAACTGAGAAGAGGATCA+TGG 0.614752 4.3:-43151734 None:intergenic
TATGCAGAAAACTCCTCCTA+GGG 0.614927 4.3:-43151671 None:intergenic
AATGGAAGGTTCTCATGTGA+GGG 0.615898 4.3:-43150464 None:intergenic
CTAATCAACTCATACACCAA+AGG 0.617873 4.3:-43150906 None:intergenic
GCCATTAGTCCCGATCATCC+GGG 0.620270 4.3:+43150536 MS.gene007704:CDS
AGATATCCATGATAAATGGA+AGG 0.625047 4.3:-43150478 None:intergenic
TGAAGCAACTGATGACATGA+AGG 0.627231 4.3:-43150349 None:intergenic
GCTGGTTGGGGTAATATGGG+TGG 0.628336 4.3:+43150861 MS.gene007704:CDS
TGGTAATGCAAATTTGGCTG+TGG 0.639028 4.3:-43150391 None:intergenic
TGATGACATGCAACCAACCA+GGG 0.645673 4.3:-43150977 None:intergenic
CTCAGCAATGACATTGTCAG+TGG 0.646298 4.3:-43151268 None:intergenic
AAATCCGACGTTGGTAACGC+CGG 0.653244 4.3:+43150576 MS.gene007704:CDS
CATAGAGTAACCATAAAGCA+GGG 0.662197 4.3:-43151232 None:intergenic
TGCTAAAGACAAGTTCACAA+AGG 0.663793 4.3:+43151076 MS.gene007704:CDS
TTCTGAGAGAGGTAAGCGTG+TGG 0.670907 4.3:+43152110 MS.gene007704:CDS
AACCTCACGAAATCCGACGT+TGG 0.679989 4.3:+43150567 MS.gene007704:CDS
GTTGGAGCACAAGCTGCATG+TGG 0.687636 4.3:+43151751 MS.gene007704:CDS
AGGAAAGCACAACCATAACG+TGG 0.709978 4.3:-43150666 None:intergenic
CAAAGGCATAATAAGCTGAG+TGG 0.711499 4.3:-43150889 None:intergenic
GACTCCGGCGTTACCAACGT+CGG 0.726114 4.3:-43150580 None:intergenic
AGGAACATGCTACCCCACTG+TGG 0.730068 4.3:-43151976 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGTTTAATAGTAAAATTAT+TGG + chr4.3:43150819-43150838 MS.gene007704:CDS 10.0%
!! TCAAAGTATATTAAATAATT+TGG + chr4.3:43151148-43151167 MS.gene007704:intron 10.0%
!! AAAAAATTGTGAGTCTAATT+CGG - chr4.3:43151426-43151445 None:intergenic 20.0%
!! AAAATTATTGGACTTGTAAA+TGG + chr4.3:43150831-43150850 MS.gene007704:CDS 20.0%
!! CTAATAAATGGATATAAAAC+AGG + chr4.3:43151497-43151516 MS.gene007704:intron 20.0%
!! TCTAACAAATCTCTAATAAA+TGG + chr4.3:43151485-43151504 MS.gene007704:intron 20.0%
!!! CACAATTTTTTTTAAAACCT+AGG + chr4.3:43151435-43151454 MS.gene007704:intron 20.0%
!!! TTGGTTATGATTTTTTTTTC+AGG + chr4.3:43151167-43151186 MS.gene007704:intron 20.0%
! AAAGATCTTCTTGATATGAA+TGG - chr4.3:43151796-43151815 None:intergenic 25.0%
! AAATATCAACTGTGTTAATC+CGG - chr4.3:43151863-43151882 None:intergenic 25.0%
! AGAAAGATATCCATGATAAA+TGG - chr4.3:43150485-43150504 None:intergenic 25.0%
! CTATAACAAATTATAGGACA+TGG + chr4.3:43151202-43151221 MS.gene007704:CDS 25.0%
! GGTATGCTATAACAAATTAT+AGG + chr4.3:43151196-43151215 MS.gene007704:CDS 25.0%
! TGAAAAGAGTTTGAAAACTT+TGG - chr4.3:43150436-43150455 None:intergenic 25.0%
! TGTAGATATATACAATCTTG+AGG - chr4.3:43151348-43151367 None:intergenic 25.0%
! TTCATATCAAGAAGATCTTT+AGG + chr4.3:43151796-43151815 MS.gene007704:CDS 25.0%
!! AGATCTTTAGGAATAATATC+AGG + chr4.3:43151808-43151827 MS.gene007704:CDS 25.0%
!! AGTTTTCAAACTCTTTTCAA+TGG + chr4.3:43150437-43150456 MS.gene007704:CDS 25.0%
!! CGGATCATAATTTTAAATTG+TGG - chr4.3:43151406-43151425 None:intergenic 25.0%
AACACTATTACACTTCTGAA+TGG + chr4.3:43152025-43152044 MS.gene007704:CDS 30.0%
AATGAAGATGAAGTACAGAA+AGG + chr4.3:43152048-43152067 MS.gene007704:CDS 30.0%
AATGACATACAAAACACTGT+TGG - chr4.3:43150708-43150727 None:intergenic 30.0%
ACATAGTACTCATCCAATAT+TGG - chr4.3:43150803-43150822 None:intergenic 30.0%
AGATATCCATGATAAATGGA+AGG - chr4.3:43150481-43150500 None:intergenic 30.0%
ATAGCAGTTAGGTTTATGAT+TGG + chr4.3:43150750-43150769 MS.gene007704:CDS 30.0%
ATGAAGATGAAGTACAGAAA+GGG + chr4.3:43152049-43152068 MS.gene007704:CDS 30.0%
CATTTGTTTCATGCCAATAT+TGG + chr4.3:43150787-43150806 MS.gene007704:CDS 30.0%
GGAATAATATCAGGTTTAAC+TGG + chr4.3:43151817-43151836 MS.gene007704:CDS 30.0%
TTAAATTACAACTGCAGTTG+CGG + chr4.3:43151379-43151398 MS.gene007704:intron 30.0%
! ATCAACTGGTAATGCAAATT+TGG - chr4.3:43150400-43150419 None:intergenic 30.0%
! ATTTGTTCATTTTCCACAGT+GGG + chr4.3:43151963-43151982 MS.gene007704:CDS 30.0%
! TCAAAATTTTCAACAGCTTC+AGG + chr4.3:43151892-43151911 MS.gene007704:CDS 30.0%
! TCTTCATTGATCTCTTTTGA+AGG - chr4.3:43152006-43152025 None:intergenic 30.0%
!! GTTTATGATTGGTTTCTCTT+TGG + chr4.3:43150761-43150780 MS.gene007704:CDS 30.0%
!! TTTCAGGTAGTATTTTCTCA+AGG + chr4.3:43150610-43150629 MS.gene007704:CDS 30.0%
!!! GATTTTTTTTTCAGGTGTTG+TGG + chr4.3:43151175-43151194 MS.gene007704:intron 30.0%
!!! GGTATTTTGGTCTTAACATT+AGG + chr4.3:43151002-43151021 MS.gene007704:CDS 30.0%
AACTATGTTTGCCATTCCAA+AGG - chr4.3:43151563-43151582 None:intergenic 35.0%
AATGTTCGTTACAATCTCCT+AGG - chr4.3:43151455-43151474 None:intergenic 35.0%
CATAGAGTAACCATAAAGCA+GGG - chr4.3:43151235-43151254 None:intergenic 35.0%
CTAATCAACTCATACACCAA+AGG - chr4.3:43150909-43150928 None:intergenic 35.0%
CTGCTTTATGGTTACTCTAT+GGG + chr4.3:43151234-43151253 MS.gene007704:CDS 35.0%
GATGTTGCAGCAAGATTATT+CGG + chr4.3:43151610-43151629 MS.gene007704:CDS 35.0%
GATTTGTGTTCAGAATCAAC+TGG - chr4.3:43150414-43150433 None:intergenic 35.0%
GGAAAATGAACAAATGCAAC+AGG - chr4.3:43151958-43151977 None:intergenic 35.0%
TGAGAACCTTCCATTTATCA+TGG + chr4.3:43150472-43150491 MS.gene007704:CDS 35.0%
TGCTAAAGACAAGTTCACAA+AGG + chr4.3:43151076-43151095 MS.gene007704:CDS 35.0%
TTGGTGCAGTTAGCATAATA+AGG - chr4.3:43150689-43150708 None:intergenic 35.0%
! AGTTCACAAAGGTTAGTTAC+TGG + chr4.3:43151087-43151106 MS.gene007704:intron 35.0%
! ATTTTCTCAAGGCTTGCAAT+GGG + chr4.3:43150621-43150640 MS.gene007704:CDS 35.0%
! CATTTGTTCATTTTCCACAG+TGG + chr4.3:43151962-43151981 MS.gene007704:CDS 35.0%
! TATTTTCTCAAGGCTTGCAA+TGG + chr4.3:43150620-43150639 MS.gene007704:CDS 35.0%
! TCATTTGTTGAAAATGCTGC+TGG + chr4.3:43150723-43150742 MS.gene007704:CDS 35.0%
! TTGCTGAGTATTTCTATGAC+AGG + chr4.3:43151283-43151302 MS.gene007704:CDS 35.0%
! TTTGTTCATTTTCCACAGTG+GGG + chr4.3:43151964-43151983 MS.gene007704:CDS 35.0%
!! TATGAGTTGATTAGAAGAGC+TGG + chr4.3:43150915-43150934 MS.gene007704:CDS 35.0%
!! TCATTGATCTCTTTTGAAGG+TGG - chr4.3:43152003-43152022 None:intergenic 35.0%
!!! AGAATTGCCTTTTTTATCCC+TGG + chr4.3:43150960-43150979 MS.gene007704:CDS 35.0%
AAATGGAAGGTTCTCATGTG+AGG - chr4.3:43150468-43150487 None:intergenic 40.0%
AACATAGTTGCTCGTCCATT+CGG + chr4.3:43151574-43151593 MS.gene007704:CDS 40.0%
AACCATAACGTGGACCTAAT+AGG - chr4.3:43150659-43150678 None:intergenic 40.0%
AATGGAAGGTTCTCATGTGA+GGG - chr4.3:43150467-43150486 None:intergenic 40.0%
AATTTGGCTGTGGTATCAGT+TGG - chr4.3:43150384-43150403 None:intergenic 40.0%
ATATCAGGTTTAACTGGTGC+AGG + chr4.3:43151823-43151842 MS.gene007704:CDS 40.0%
ATATGCAGAAAACTCCTCCT+AGG - chr4.3:43151675-43151694 None:intergenic 40.0%
ATCATGGATAGTACTGCGAT+CGG - chr4.3:43151721-43151740 None:intergenic 40.0%
CAACCAACCAGGGATAAAAA+AGG - chr4.3:43150970-43150989 None:intergenic 40.0%
CACTCAGCTTATTATGCCTT+TGG + chr4.3:43150890-43150909 MS.gene007704:CDS 40.0%
CCATAGAGTAACCATAAAGC+AGG - chr4.3:43151236-43151255 None:intergenic 40.0%
CCTGCTTTATGGTTACTCTA+TGG + chr4.3:43151233-43151252 MS.gene007704:CDS 40.0%
GATTTACCTGATGGTAACCT+TGG + chr4.3:43151029-43151048 MS.gene007704:CDS 40.0%
GGTGCAATTTGTGACCTATT+AGG + chr4.3:43150642-43150661 MS.gene007704:CDS 40.0%
GGTTACCATCAGGTAAATCT+TGG - chr4.3:43151028-43151047 None:intergenic 40.0%
TATGCAGAAAACTCCTCCTA+GGG - chr4.3:43151674-43151693 None:intergenic 40.0%
TATGTTTGCCATTCCAAAGG+AGG - chr4.3:43151560-43151579 None:intergenic 40.0%
TGAAGCAACTGATGACATGA+AGG - chr4.3:43150352-43150371 None:intergenic 40.0%
TTAGGCCAAGATTTACCTGA+TGG + chr4.3:43151020-43151039 MS.gene007704:CDS 40.0%
TTCTCAGCAAACCTAAGACT+TGG - chr4.3:43152084-43152103 None:intergenic 40.0%
! CAAAGGCATAATAAGCTGAG+TGG - chr4.3:43150892-43150911 None:intergenic 40.0%
! CTGCTGGTTATATAGCAGTT+AGG + chr4.3:43150739-43150758 MS.gene007704:CDS 40.0%
! GCATGTCATCATGGGTATTT+TGG + chr4.3:43150989-43151008 MS.gene007704:CDS 40.0%
! GGAGTTTTCTGCATATGGTT+AGG + chr4.3:43151679-43151698 MS.gene007704:CDS 40.0%
! TAGGAGGAGTTTTCTGCATA+TGG + chr4.3:43151674-43151693 MS.gene007704:CDS 40.0%
! TAGTGGGTAGGAGTTGTATT+TGG - chr4.3:43152153-43152172 None:intergenic 40.0%
! TGGATTTTTGCCCTGCTTTA+TGG + chr4.3:43151222-43151241 MS.gene007704:CDS 40.0%
! TGGTAATGCAAATTTGGCTG+TGG - chr4.3:43150394-43150413 None:intergenic 40.0%
!! TGATCTCTTTTGAAGGTGGA+AGG - chr4.3:43151999-43152018 None:intergenic 40.0%
!!! TTGCCTTTTTTATCCCTGGT+TGG + chr4.3:43150964-43150983 MS.gene007704:CDS 40.0%
AAAGGAGGCAGCAATGATTC+CGG - chr4.3:43151545-43151564 None:intergenic 45.0%
AACCTAAAGCTACACACAGC+CGG + chr4.3:43151523-43151542 MS.gene007704:CDS 45.0%
AGAAAACTCCTCCTAGGGTT+TGG - chr4.3:43151669-43151688 None:intergenic 45.0%
AGCTTCAGGTCTTTCATGGA+TGG + chr4.3:43151906-43151925 MS.gene007704:CDS 45.0%
ATGATGACATGCAACCAACC+AGG - chr4.3:43150981-43151000 None:intergenic 45.0%
ATGTTTCGTCTCGACATTCG+CGG + chr4.3:43150509-43150528 MS.gene007704:CDS 45.0%
CAACAGCTTCAGGTCTTTCA+TGG + chr4.3:43151902-43151921 MS.gene007704:CDS 45.0%
CTCAGCAATGACATTGTCAG+TGG - chr4.3:43151271-43151290 None:intergenic 45.0%
CTTGTAAATGGTACTGCTGC+TGG + chr4.3:43150843-43150862 MS.gene007704:CDS 45.0%
GACCTATTAGGTCCACGTTA+TGG + chr4.3:43150654-43150673 MS.gene007704:CDS 45.0%
GAGGAAGACTATGGACACTA+TGG + chr4.3:43151638-43151657 MS.gene007704:CDS 45.0%
GCAAGATTATTCGGCATGAG+AGG + chr4.3:43151619-43151638 MS.gene007704:CDS 45.0%
GCAATTCTCCAAGCAGTGAA+AGG - chr4.3:43150948-43150967 None:intergenic 45.0%
GCTTCAGGTCTTTCATGGAT+GGG + chr4.3:43151907-43151926 MS.gene007704:CDS 45.0%
GTCATCAGTTGCTTCACCAA+TGG + chr4.3:43150356-43150375 MS.gene007704:CDS 45.0%
GTGGTATCAGTTGGAACCAT+TGG - chr4.3:43150375-43150394 None:intergenic 45.0%
TAAATGGTACTGCTGCTGGT+TGG + chr4.3:43150847-43150866 MS.gene007704:CDS 45.0%
TCCAACTGAGAAGAGGATCA+TGG - chr4.3:43151737-43151756 None:intergenic 45.0%
TCCATGATCCTCTTCTCAGT+TGG + chr4.3:43151733-43151752 MS.gene007704:CDS 45.0%
TCTTCCAGTTACTCCATGCA+TGG - chr4.3:43150322-43150341 None:intergenic 45.0%
TGATGACATGCAACCAACCA+GGG - chr4.3:43150980-43150999 None:intergenic 45.0%
TGGATGGGTGCAATGATTGT+TGG + chr4.3:43151922-43151941 MS.gene007704:CDS 45.0%
TGGGTAGGAGTTGTATTTGG+TGG - chr4.3:43152150-43152169 None:intergenic 45.0%
TTAAGGTTGTCCCGGATGAT+CGG - chr4.3:43150549-43150568 None:intergenic 45.0%
! AAAGCACCAAGGTTACCATC+AGG - chr4.3:43151038-43151057 None:intergenic 45.0%
! AAATGGTACTGCTGCTGGTT+GGG + chr4.3:43150848-43150867 MS.gene007704:CDS 45.0%
! AGGAAAGCACAACCATAACG+TGG - chr4.3:43150669-43150688 None:intergenic 45.0%
! GGTTGGTTGCATGTCATCAT+GGG + chr4.3:43150981-43151000 MS.gene007704:CDS 45.0%
! TGGTTGGTTGCATGTCATCA+TGG + chr4.3:43150980-43150999 MS.gene007704:CDS 45.0%
!! CTTGGTGCTTTGCAGAAGAA+GGG + chr4.3:43151047-43151066 MS.gene007704:CDS 45.0%
!! GAGAATAGTCGTTCTGAGAG+AGG + chr4.3:43152099-43152118 MS.gene007704:CDS 45.0%
AAACTCCTCCTAGGGTTTGG+AGG - chr4.3:43151666-43151685 None:intergenic 50.0%
AACCTCACGAAATCCGACGT+TGG + chr4.3:43150567-43150586 MS.gene007704:CDS 50.0%
ACTGGTGCAGGTGGAAACTT+TGG + chr4.3:43151835-43151854 MS.gene007704:CDS 50.0%
AGCTCCATGCATGGAGTAAC+TGG + chr4.3:43150315-43150334 MS.gene007704:CDS 50.0%
ATAGTTGCTCGTCCATTCGG+CGG + chr4.3:43151577-43151596 MS.gene007704:CDS 50.0%
CGTCGGATTTCGTGAGGTTA+AGG - chr4.3:43150566-43150585 None:intergenic 50.0%
CTATGGATCCTCCAAACCCT+AGG + chr4.3:43151655-43151674 MS.gene007704:CDS 50.0%
CTTGTGCTCCAACTGAGAAG+AGG - chr4.3:43151744-43151763 None:intergenic 50.0%
GCTGATATTGAGGGTTCACC+TGG + chr4.3:43150291-43150310 MS.gene007704:CDS 50.0%
GCTGCATGTGGTGCAACATT+TGG + chr4.3:43151763-43151782 MS.gene007704:CDS 50.0%
GGAATCATTGCTGCCTCCTT+TGG + chr4.3:43151544-43151563 MS.gene007704:CDS 50.0%
GGTTGCATCAGCCAAGTCTT+AGG + chr4.3:43152070-43152089 MS.gene007704:CDS 50.0%
GTTGGGGTAATATGGGTGGT+GGG + chr4.3:43150865-43150884 MS.gene007704:CDS 50.0%
TAAGGTTGTCCCGGATGATC+GGG - chr4.3:43150548-43150567 None:intergenic 50.0%
TACCAACGTCGGATTTCGTG+AGG - chr4.3:43150572-43150591 None:intergenic 50.0%
TCAGGTTTAACTGGTGCAGG+TGG + chr4.3:43151826-43151845 MS.gene007704:CDS 50.0%
TCGGCATGAGAGGAAGACTA+TGG + chr4.3:43151629-43151648 MS.gene007704:CDS 50.0%
TCGTGAGGTTAAGGTTGTCC+CGG - chr4.3:43150557-43150576 None:intergenic 50.0%
TCTGATGCATATCCGCCGAA+TGG - chr4.3:43151592-43151611 None:intergenic 50.0%
TTCCGGCTGTGTGTAGCTTT+AGG - chr4.3:43151528-43151547 None:intergenic 50.0%
TTGGGGTAATATGGGTGGTG+GGG + chr4.3:43150866-43150885 MS.gene007704:CDS 50.0%
! AATGGTACTGCTGCTGGTTG+GGG + chr4.3:43150849-43150868 MS.gene007704:CDS 50.0%
! CATTGCTGCCTCCTTTGGAA+TGG + chr4.3:43151549-43151568 MS.gene007704:CDS 50.0%
! CCTTCTTCTGCAAAGCACCA+AGG - chr4.3:43151049-43151068 None:intergenic 50.0%
! GCAGGTGGAAACTTTGGTTC+CGG + chr4.3:43151841-43151860 MS.gene007704:CDS 50.0%
! GCTGCTGGTTGGGGTAATAT+GGG + chr4.3:43150858-43150877 MS.gene007704:CDS 50.0%
! GTGCTACTCCTTTCACTGCT+TGG + chr4.3:43150937-43150956 MS.gene007704:CDS 50.0%
! TGCTGCTGGTTGGGGTAATA+TGG + chr4.3:43150857-43150876 MS.gene007704:CDS 50.0%
! TTCTGAGAGAGGTAAGCGTG+TGG + chr4.3:43152110-43152129 MS.gene007704:CDS 50.0%
!! AAATCCGACGTTGGTAACGC+CGG + chr4.3:43150576-43150595 MS.gene007704:CDS 50.0%
!! CCTTGGTGCTTTGCAGAAGA+AGG + chr4.3:43151046-43151065 MS.gene007704:CDS 50.0%
ACCTGAAACCGAAGCGACTC+CGG - chr4.3:43150598-43150617 None:intergenic 55.0%
ACTCCATGCATGGAGCTTCC+AGG - chr4.3:43150312-43150331 None:intergenic 55.0%
AGGAACATGCTACCCCACTG+TGG - chr4.3:43151979-43151998 None:intergenic 55.0%
CGCCATTAGTCCCGATCATC+CGG + chr4.3:43150535-43150554 MS.gene007704:CDS 55.0%
GCCATTAGTCCCGATCATCC+GGG + chr4.3:43150536-43150555 MS.gene007704:CDS 55.0%
GCTGGTTGGGGTAATATGGG+TGG + chr4.3:43150861-43150880 MS.gene007704:CDS 55.0%
GGTTGGGGTAATATGGGTGG+TGG + chr4.3:43150864-43150883 MS.gene007704:CDS 55.0%
GTTGGAGCACAAGCTGCATG+TGG + chr4.3:43151751-43151770 MS.gene007704:CDS 55.0%
TCACCTGGAAGCTCCATGCA+TGG + chr4.3:43150306-43150325 MS.gene007704:CDS 55.0%
TCCCGGATGATCGGGACTAA+TGG - chr4.3:43150540-43150559 None:intergenic 55.0%
TGGATCCTCCAAACCCTAGG+AGG + chr4.3:43151658-43151677 MS.gene007704:CDS 55.0%
GACTCCGGCGTTACCAACGT+CGG - chr4.3:43150583-43150602 None:intergenic 60.0%
! TGGTAACGCCGGAGTCGCTT+CGG + chr4.3:43150587-43150606 MS.gene007704:CDS 60.0%
GCCGGAGTCGCTTCGGTTTC+AGG + chr4.3:43150594-43150613 MS.gene007704:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 43150288 43152173 43150288 ID=MS.gene007704
chr4.3 mRNA 43150288 43152173 43150288 ID=MS.gene007704.t1;Parent=MS.gene007704
chr4.3 exon 43150288 43151097 43150288 ID=MS.gene007704.t1.exon1;Parent=MS.gene007704.t1
chr4.3 CDS 43150288 43151097 43150288 ID=cds.MS.gene007704.t1;Parent=MS.gene007704.t1
chr4.3 exon 43151189 43151304 43151189 ID=MS.gene007704.t1.exon2;Parent=MS.gene007704.t1
chr4.3 CDS 43151189 43151304 43151189 ID=cds.MS.gene007704.t1;Parent=MS.gene007704.t1
chr4.3 exon 43151519 43152173 43151519 ID=MS.gene007704.t1.exon3;Parent=MS.gene007704.t1
chr4.3 CDS 43151519 43152173 43151519 ID=cds.MS.gene007704.t1;Parent=MS.gene007704.t1
Gene Sequence

>MS.gene007704

ATGGCTGATATTGAGGGTTCACCTGGAAGCTCCATGCATGGAGTAACTGGAAGAGAACAAACCTTCATGTCATCAGTTGCTTCACCAATGGTTCCAACTGATACCACAGCCAAATTTGCATTACCAGTTGATTCTGAACACAAATCCAAAGTTTTCAAACTCTTTTCAATGGCTAACCCTCACATGAGAACCTTCCATTTATCATGGATATCTTTCTTCACATGTTTCGTCTCGACATTCGCGGCAGCGCCATTAGTCCCGATCATCCGGGACAACCTTAACCTCACGAAATCCGACGTTGGTAACGCCGGAGTCGCTTCGGTTTCAGGTAGTATTTTCTCAAGGCTTGCAATGGGTGCAATTTGTGACCTATTAGGTCCACGTTATGGTTGTGCTTTCCTTATTATGCTAACTGCACCAACAGTGTTTTGTATGTCATTTGTTGAAAATGCTGCTGGTTATATAGCAGTTAGGTTTATGATTGGTTTCTCTTTGGCAACATTTGTTTCATGCCAATATTGGATGAGTACTATGTTTAATAGTAAAATTATTGGACTTGTAAATGGTACTGCTGCTGGTTGGGGTAATATGGGTGGTGGGGCCACTCAGCTTATTATGCCTTTGGTGTATGAGTTGATTAGAAGAGCTGGTGCTACTCCTTTCACTGCTTGGAGAATTGCCTTTTTTATCCCTGGTTGGTTGCATGTCATCATGGGTATTTTGGTCTTAACATTAGGCCAAGATTTACCTGATGGTAACCTTGGTGCTTTGCAGAAGAAGGGTAATGTTGCTAAAGACAAGTTCACAAAGGTTAGTTACTGGTTTACACAATTAATCAGTCGAGTTAAGAAATTTTAAGATCAAAGTATATTAAATAATTTGGTTATGATTTTTTTTTCAGGTGTTGTGGTATGCTATAACAAATTATAGGACATGGATTTTTGCCCTGCTTTATGGTTACTCTATGGGAGTTGAATTAACCACTGACAATGTCATTGCTGAGTATTTCTATGACAGGTAATTAGTTGAGATGCTTAAACTCGTACAACTTTTAAGTCCTCAAGATTGTATATATCTACATGCTTAATGTTTTAAATTACAACTGCAGTTGCGGACCACAATTTAAAATTATGATCCGAATTAGACTCACAATTTTTTTTAAAACCTAGGAGATTGTAACGAACATTTTTTCTTTTTTCTAACAAATCTCTAATAAATGGATATAAAACAGGTTTAACCTAAAGCTACACACAGCCGGAATCATTGCTGCCTCCTTTGGAATGGCAAACATAGTTGCTCGTCCATTCGGCGGATATGCATCAGATGTTGCAGCAAGATTATTCGGCATGAGAGGAAGACTATGGACACTATGGATCCTCCAAACCCTAGGAGGAGTTTTCTGCATATGGTTAGGACGTGCGAATACACTTCCGATCGCAGTACTATCCATGATCCTCTTCTCAGTTGGAGCACAAGCTGCATGTGGTGCAACATTTGGAATCATTCCATTCATATCAAGAAGATCTTTAGGAATAATATCAGGTTTAACTGGTGCAGGTGGAAACTTTGGTTCCGGATTAACACAGTTGATATTTTTTTCAAGCTCAAAATTTTCAACAGCTTCAGGTCTTTCATGGATGGGTGCAATGATTGTTGGTTGTACACTTCCTGTTGCATTTGTTCATTTTCCACAGTGGGGTAGCATGTTCCTTCCACCTTCAAAAGAGATCAATGAAGAACACTATTACACTTCTGAATGGAATGAAGATGAAGTACAGAAAGGGTTGCATCAGCCAAGTCTTAGGTTTGCTGAGAATAGTCGTTCTGAGAGAGGTAAGCGTGTGGCTTCTGCAGCAACACCACCAAATACAACTCCTACCCACTAG
Protein sequence

>MS.gene007704.t1

MADIEGSPGSSMHGVTGREQTFMSSVASPMVPTDTTAKFALPVDSEHKSKVFKLFSMANPHMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKSDVGNAGVASVSGSIFSRLAMGAICDLLGPRYGCAFLIMLTAPTVFCMSFVENAAGYIAVRFMIGFSLATFVSCQYWMSTMFNSKIIGLVNGTAAGWGNMGGGATQLIMPLVYELIRRAGATPFTAWRIAFFIPGWLHVIMGILVLTLGQDLPDGNLGALQKKGNVAKDKFTKVLWYAITNYRTWIFALLYGYSMGVELTTDNVIAEYFYDRFNLKLHTAGIIAASFGMANIVARPFGGYASDVAARLFGMRGRLWTLWILQTLGGVFCIWLGRANTLPIAVLSMILFSVGAQAACGATFGIIPFISRRSLGIISGLTGAGGNFGSGLTQLIFFSSSKFSTASGLSWMGAMIVGCTLPVAFVHFPQWGSMFLPPSKEINEEHYYTSEWNEDEVQKGLHQPSLRFAENSRSERGKRVASAATPPNTTPTH