Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene008905.t1 | XP_013446118.1 | 99.4 | 348 | 2 | 0 | 1 | 348 | 1 | 348 | 1.00E-181 | 646 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene008905.t1 | Q38942 | 84.8 | 349 | 52 | 1 | 1 | 348 | 1 | 349 | 3.6e-159 | 562.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene008905.t1 | A0A072U2U6 | 99.4 | 348 | 2 | 0 | 1 | 348 | 1 | 348 | 7.4e-182 | 646.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene008905 | MS.gene000251 | PPI |
| MS.gene008905 | MS.gene86831 | PPI |
| MS.gene008905 | MS.gene55320 | PPI |
| MS.gene008905 | MS.gene85897 | PPI |
| MS.gene008905 | MS.gene066387 | PPI |
| MS.gene92360 | MS.gene008905 | PPI |
| MS.gene022537 | MS.gene008905 | PPI |
| MS.gene008905 | MS.gene79186 | PPI |
| MS.gene008905 | MS.gene00617 | PPI |
| MS.gene008905 | MS.gene060067 | PPI |
| MS.gene065809 | MS.gene008905 | PPI |
| MS.gene024963 | MS.gene008905 | PPI |
| MS.gene07517 | MS.gene008905 | PPI |
| MS.gene060071 | MS.gene008905 | PPI |
| MS.gene060069 | MS.gene008905 | PPI |
| MS.gene008905 | MS.gene072058 | PPI |
| MS.gene008905 | MS.gene004979 | PPI |
| MS.gene008905 | MS.gene047200 | PPI |
| MS.gene008905 | MS.gene79188 | PPI |
| MS.gene53114 | MS.gene008905 | PPI |
| MS.gene62514 | MS.gene008905 | PPI |
| MS.gene008905 | MS.gene046944 | PPI |
| MS.gene008905 | MS.gene61568 | PPI |
| MS.gene008905 | MS.gene40528 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene008905.t1 | MTR_8g068875 | 99.138 | 348 | 3 | 0 | 1 | 348 | 1 | 348 | 0.0 | 733 |
| MS.gene008905.t1 | MTR_2g008410 | 85.075 | 335 | 49 | 1 | 15 | 348 | 17 | 351 | 0.0 | 626 |
| MS.gene008905.t1 | MTR_1g492860 | 86.207 | 348 | 46 | 1 | 1 | 348 | 1 | 346 | 0.0 | 626 |
| MS.gene008905.t1 | MTR_2g008410 | 80.597 | 335 | 46 | 2 | 15 | 348 | 17 | 333 | 0.0 | 582 |
| MS.gene008905.t1 | MTR_2g008410 | 88.070 | 285 | 34 | 0 | 64 | 348 | 2 | 286 | 0.0 | 555 |
| MS.gene008905.t1 | MTR_2g008410 | 82.807 | 285 | 31 | 1 | 64 | 348 | 2 | 268 | 0.0 | 510 |
| MS.gene008905.t1 | MTR_5g005120 | 34.524 | 336 | 197 | 7 | 18 | 342 | 15 | 338 | 2.78e-63 | 205 |
| MS.gene008905.t1 | MTR_5g043000 | 27.586 | 319 | 216 | 7 | 8 | 319 | 2 | 312 | 1.65e-28 | 113 |
| MS.gene008905.t1 | MTR_2g436510 | 24.516 | 310 | 191 | 11 | 13 | 309 | 159 | 438 | 2.85e-13 | 70.9 |
| MS.gene008905.t1 | MTR_6g004040 | 34.211 | 114 | 70 | 1 | 28 | 141 | 105 | 213 | 7.95e-12 | 67.0 |
| MS.gene008905.t1 | MTR_2g012630 | 33.333 | 114 | 71 | 1 | 28 | 141 | 104 | 212 | 1.24e-11 | 66.2 |
| MS.gene008905.t1 | MTR_2g012630 | 33.333 | 114 | 71 | 1 | 28 | 141 | 104 | 212 | 1.39e-11 | 66.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene008905.t1 | AT1G80670 | 83.668 | 349 | 56 | 1 | 1 | 348 | 1 | 349 | 0.0 | 633 |
| MS.gene008905.t1 | AT1G15850 | 72.143 | 140 | 37 | 1 | 1 | 138 | 1 | 140 | 5.39e-68 | 210 |
| MS.gene008905.t1 | AT3G19590 | 35.362 | 345 | 200 | 8 | 9 | 342 | 2 | 334 | 2.02e-64 | 208 |
| MS.gene008905.t1 | AT1G49910 | 33.724 | 341 | 203 | 8 | 13 | 342 | 5 | 333 | 3.75e-60 | 197 |
| MS.gene008905.t1 | AT1G69400 | 26.059 | 307 | 207 | 7 | 18 | 315 | 7 | 302 | 5.68e-29 | 114 |
| MS.gene008905.t1 | AT1G69400 | 25.197 | 254 | 166 | 7 | 18 | 258 | 7 | 249 | 1.09e-21 | 93.6 |
| MS.gene008905.t1 | AT1G11160 | 34.211 | 114 | 70 | 1 | 28 | 141 | 104 | 212 | 1.85e-13 | 72.0 |
| MS.gene008905.t1 | AT1G11160 | 24.583 | 240 | 160 | 8 | 68 | 299 | 12 | 238 | 8.00e-13 | 70.1 |
| MS.gene008905.t1 | AT1G11160 | 34.211 | 114 | 70 | 1 | 28 | 141 | 62 | 170 | 1.86e-13 | 72.0 |
| MS.gene008905.t1 | AT1G11160 | 34.211 | 114 | 70 | 1 | 28 | 141 | 104 | 212 | 1.91e-13 | 72.0 |
| MS.gene008905.t1 | AT1G11160 | 24.583 | 240 | 160 | 8 | 68 | 299 | 12 | 238 | 7.88e-13 | 70.1 |
| MS.gene008905.t1 | AT1G11160 | 34.211 | 114 | 70 | 1 | 28 | 141 | 104 | 212 | 1.99e-13 | 72.0 |
| MS.gene008905.t1 | AT1G11160 | 24.583 | 240 | 160 | 8 | 68 | 299 | 12 | 238 | 9.40e-13 | 70.1 |
| MS.gene008905.t1 | AT1G61210 | 34.211 | 114 | 70 | 1 | 28 | 141 | 104 | 212 | 3.23e-13 | 71.2 |
| MS.gene008905.t1 | AT4G15900 | 23.871 | 310 | 193 | 10 | 13 | 309 | 165 | 444 | 3.50e-13 | 70.9 |
| MS.gene008905.t1 | AT1G61210 | 34.211 | 114 | 70 | 1 | 28 | 141 | 104 | 212 | 5.10e-13 | 70.9 |
| MS.gene008905.t1 | AT1G61210 | 34.211 | 114 | 70 | 1 | 28 | 141 | 104 | 212 | 5.15e-13 | 70.9 |
| MS.gene008905.t1 | AT5G23430 | 33.628 | 113 | 70 | 1 | 29 | 141 | 106 | 213 | 3.09e-12 | 68.2 |
| MS.gene008905.t1 | AT5G23430 | 33.628 | 113 | 70 | 1 | 29 | 141 | 106 | 213 | 3.32e-12 | 68.2 |
| MS.gene008905.t1 | AT5G23430 | 33.628 | 113 | 70 | 1 | 29 | 141 | 106 | 213 | 3.32e-12 | 68.2 |
| MS.gene008905.t1 | AT5G67320 | 21.656 | 314 | 213 | 7 | 13 | 303 | 254 | 557 | 3.53e-12 | 67.8 |
| MS.gene008905.t1 | AT3G16650 | 22.876 | 306 | 200 | 9 | 13 | 309 | 159 | 437 | 4.07e-12 | 67.4 |
| MS.gene008905.t1 | AT5G08390 | 33.628 | 113 | 70 | 1 | 29 | 141 | 106 | 213 | 5.08e-12 | 67.8 |
| MS.gene008905.t1 | AT5G08390 | 24.583 | 240 | 160 | 8 | 68 | 299 | 13 | 239 | 4.83e-11 | 64.7 |
Find 80 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CACACCTTGTCTGATATTTC+AGG | 0.213527 | 8.2:-34052098 | None:intergenic |
| TATTACCGGATGGAAATTTA+AGG | 0.288149 | 8.2:-34052796 | None:intergenic |
| GTCAACAGACCGAGTATAAA+AGG | 0.294586 | 8.2:+34052064 | MS.gene008905:intron |
| GCATACCGCGTAAGCATATA+TGG | 0.318779 | 8.2:-34053163 | None:intergenic |
| CCGATCAACATGTCTTCTTT+CGG | 0.324051 | 8.2:+34048816 | None:intergenic |
| GTCTATAGGTTTGCTATGAT+TGG | 0.329481 | 8.2:+34056006 | MS.gene008905:intron |
| ATTACCTCATAGGATTTGTT+TGG | 0.347710 | 8.2:-34048861 | None:intergenic |
| TGAAGTAGCGACAAGGAAAT+TGG | 0.350526 | 8.2:-34049016 | None:intergenic |
| GAACAGGAGACTGAGGTTAA+AGG | 0.352225 | 8.2:+34056321 | MS.gene008905:intron |
| ATTTCCTTGTCGCTACTTCA+TGG | 0.367802 | 8.2:+34049019 | MS.gene008905:CDS |
| ACTGAAAGAAAGACTTGAAA+TGG | 0.382134 | 8.2:-34048986 | None:intergenic |
| GCGACAAGGAAATTGGCTTT+AGG | 0.385636 | 8.2:-34049009 | None:intergenic |
| ATGTAGTTCTTTGCTGTAGT+TGG | 0.387737 | 8.2:-34056053 | None:intergenic |
| TTCGTAATAGGTTCGGTGTT+GGG | 0.411339 | 8.2:+34049125 | MS.gene008905:intron |
| GACATTGTATAACATCGATC+AGG | 0.430312 | 8.2:-34051116 | None:intergenic |
| ATTGAATGTACCACAAGGTA+TGG | 0.430485 | 8.2:-34053130 | None:intergenic |
| AAGCCGCGAGCTGGATCAAC+AGG | 0.435624 | 8.2:+34056345 | MS.gene008905:CDS |
| GCGTTAGGTATTGGGATACT+AGG | 0.449325 | 8.2:+34051063 | MS.gene008905:intron |
| TTCTGGAGGTTGTGACAAGC+AGG | 0.452789 | 8.2:+34049360 | MS.gene008905:CDS |
| TGAAGGTGTTGTGCTCTGCT+TGG | 0.460595 | 8.2:+34049314 | MS.gene008905:intron |
| AGAAAGTAATGAGATATATT+CGG | 0.462052 | 8.2:+34052767 | MS.gene008905:CDS |
| ATCATGTGAAATTGAAGCCT+TGG | 0.468482 | 8.2:-34049184 | None:intergenic |
| TGTGGTACATTCAATAATGA+TGG | 0.470459 | 8.2:+34053138 | MS.gene008905:CDS |
| ACACCTTGTCTGATATTTCA+GGG | 0.476154 | 8.2:-34052097 | None:intergenic |
| CAAAGAGATTGCGTGGATAC+CGG | 0.479422 | 8.2:+34049447 | MS.gene008905:CDS |
| ATCCACGCAATCTCTTTGAT+AGG | 0.482444 | 8.2:-34049442 | None:intergenic |
| CTTCAACTTGCAGAATCCTC+AGG | 0.486798 | 8.2:+34051190 | MS.gene008905:CDS |
| AACGCATAACATTACCTCAT+AGG | 0.487242 | 8.2:-34048871 | None:intergenic |
| GGGAGTTGTTGAGTATGCAC+TGG | 0.487395 | 8.2:-34051095 | None:intergenic |
| TTTCCTTGTCGCTACTTCAT+GGG | 0.488482 | 8.2:+34049020 | MS.gene008905:CDS |
| TGCTCTGCTTGGAAAGATGA+TGG | 0.491720 | 8.2:+34049325 | MS.gene008905:CDS |
| TTTCGTAATAGGTTCGGTGT+TGG | 0.491966 | 8.2:+34049124 | MS.gene008905:intron |
| GTTAAAGGTAAGCCGCGAGC+TGG | 0.502221 | 8.2:+34056336 | MS.gene008905:CDS |
| CGCTACTTCATGGGACAACC+AGG | 0.504108 | 8.2:+34049029 | MS.gene008905:CDS |
| AGAAAGACTTGAAATGGAAT+CGG | 0.504515 | 8.2:-34048980 | None:intergenic |
| TGCTTTGTTGTGCATCATCA+AGG | 0.506986 | 8.2:-34052719 | None:intergenic |
| AATTGAAGCCTTGGGTGTAC+TGG | 0.515867 | 8.2:-34049175 | None:intergenic |
| GGCTCAATAGAGGGAAGGGT+TGG | 0.523184 | 8.2:+34052687 | MS.gene008905:CDS |
| CTGTAGGTTGGCTCAATAGA+GGG | 0.523662 | 8.2:+34052678 | MS.gene008905:intron |
| CCTGTAGGTTGGCTCAATAG+AGG | 0.529343 | 8.2:+34052677 | MS.gene008905:intron |
| GAACTCCTTAAATTTCCATC+CGG | 0.534710 | 8.2:+34052791 | MS.gene008905:CDS |
| TCATGTGAAATTGAAGCCTT+GGG | 0.541448 | 8.2:-34049183 | None:intergenic |
| TTTGCAACTGCTGGATCTGA+CGG | 0.543216 | 8.2:+34052936 | MS.gene008905:CDS |
| GTTTGCTATGATTGGAGCAA+AGG | 0.546194 | 8.2:+34056014 | MS.gene008905:CDS |
| GAGACATCCTCTGATGGTTG+TGG | 0.546885 | 8.2:+34051142 | MS.gene008905:CDS |
| AAATCCAAACAAATCCTATG+AGG | 0.547226 | 8.2:+34048857 | MS.gene008905:CDS |
| TTGAATGTACCACAAGGTAT+GGG | 0.565862 | 8.2:-34053129 | None:intergenic |
| CGCCCCTGAAATATCAGACA+AGG | 0.571242 | 8.2:+34052094 | MS.gene008905:CDS |
| CTCCTATCAAAGAGATTGCG+TGG | 0.576447 | 8.2:+34049440 | MS.gene008905:CDS |
| GAAGACAATCAGATTCCTAT+CGG | 0.589539 | 8.2:-34051171 | None:intergenic |
| GTGGCTAAAAGACTCATCTC+CGG | 0.590432 | 8.2:-34049466 | None:intergenic |
| TCATGCATAGCAACAGTCAC+CGG | 0.593184 | 8.2:-34049415 | None:intergenic |
| CATATATATGCACTTACCAC+AGG | 0.593393 | 8.2:+34056073 | MS.gene008905:CDS |
| AAGACTTGAAATGGAATCGG+TGG | 0.593663 | 8.2:-34048977 | None:intergenic |
| CTGATGTGAACAGGAGACTG+AGG | 0.607452 | 8.2:+34056314 | MS.gene008905:intron |
| GGTTGGCTCAATAGAGGGAA+GGG | 0.612759 | 8.2:+34052683 | MS.gene008905:intron |
| AGACATCCTCTGATGGTTGT+GGG | 0.614239 | 8.2:+34051143 | MS.gene008905:CDS |
| AGGTTGGCTCAATAGAGGGA+AGG | 0.615075 | 8.2:+34052682 | MS.gene008905:intron |
| TTGTTGAGTATGCACTGGGT+TGG | 0.621738 | 8.2:-34051090 | None:intergenic |
| GCGGTTCCCACAACCATCAG+AGG | 0.624752 | 8.2:-34051149 | None:intergenic |
| TGTTGTTACCAGTACACCCA+AGG | 0.625135 | 8.2:+34049167 | MS.gene008905:CDS |
| GGAGTTGTTGAGTATGCACT+GGG | 0.626401 | 8.2:-34051094 | None:intergenic |
| ACATTGTATAACATCGATCA+GGG | 0.631495 | 8.2:-34051115 | None:intergenic |
| CTTCCTGTTGATCCAGCTCG+CGG | 0.634887 | 8.2:-34056348 | None:intergenic |
| CTATGATTGGAGCAAAGGTG+CGG | 0.636918 | 8.2:+34056019 | MS.gene008905:CDS |
| GACAATCAGATTCCTATCGG+CGG | 0.637783 | 8.2:-34051168 | None:intergenic |
| TGGTTGTGGGAACCGCCGAT+AGG | 0.641214 | 8.2:+34051156 | MS.gene008905:CDS |
| GGACAGTAAACAGAGACTCA+AGG | 0.644768 | 8.2:+34052980 | MS.gene008905:CDS |
| CACCTTGTCTGATATTTCAG+GGG | 0.645235 | 8.2:-34052096 | None:intergenic |
| AGACTTGAAATGGAATCGGT+GGG | 0.645745 | 8.2:-34048976 | None:intergenic |
| ATATCTCATTACTTTCTCTG+TGG | 0.654168 | 8.2:-34052761 | None:intergenic |
| TGTAGTCAGCCCATACCTTG+TGG | 0.658844 | 8.2:+34053120 | MS.gene008905:CDS |
| GGTTGCCCTCCAGACAAAAG+CGG | 0.664141 | 8.2:-34049394 | None:intergenic |
| GTCAGTGAGACATCCTCTGA+TGG | 0.664484 | 8.2:+34051136 | MS.gene008905:CDS |
| GTGACAAGCAGGCTAAGATG+TGG | 0.674018 | 8.2:+34049371 | MS.gene008905:CDS |
| TGTTGGGAGATATCAAAGAA+CGG | 0.679052 | 8.2:+34049141 | MS.gene008905:CDS |
| TGTCCCATGAAGTAGCGACA+AGG | 0.689683 | 8.2:-34049023 | None:intergenic |
| CTTGAAATGGAATCGGTGGG+TGG | 0.706485 | 8.2:-34048973 | None:intergenic |
| TGGTTCCATATATGCTTACG+CGG | 0.709671 | 8.2:+34053158 | MS.gene008905:CDS |
| TCATTATTGAATGTACCACA+AGG | 0.728862 | 8.2:-34053135 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 34048825 | 34056377 | 34048825 | ID=MS.gene008905 |
| chr8.2 | mRNA | 34048825 | 34056377 | 34048825 | ID=MS.gene008905.t1;Parent=MS.gene008905 |
| chr8.2 | exon | 34048825 | 34048878 | 34048825 | ID=MS.gene008905.t1.exon1;Parent=MS.gene008905.t1 |
| chr8.2 | CDS | 34048825 | 34048878 | 34048825 | ID=cds.MS.gene008905.t1;Parent=MS.gene008905.t1 |
| chr8.2 | exon | 34048964 | 34049050 | 34048964 | ID=MS.gene008905.t1.exon2;Parent=MS.gene008905.t1 |
| chr8.2 | CDS | 34048964 | 34049050 | 34048964 | ID=cds.MS.gene008905.t1;Parent=MS.gene008905.t1 |
| chr8.2 | exon | 34049135 | 34049212 | 34049135 | ID=MS.gene008905.t1.exon3;Parent=MS.gene008905.t1 |
| chr8.2 | CDS | 34049135 | 34049212 | 34049135 | ID=cds.MS.gene008905.t1;Parent=MS.gene008905.t1 |
| chr8.2 | exon | 34049319 | 34049512 | 34049319 | ID=MS.gene008905.t1.exon4;Parent=MS.gene008905.t1 |
| chr8.2 | CDS | 34049319 | 34049512 | 34049319 | ID=cds.MS.gene008905.t1;Parent=MS.gene008905.t1 |
| chr8.2 | exon | 34051070 | 34051211 | 34051070 | ID=MS.gene008905.t1.exon5;Parent=MS.gene008905.t1 |
| chr8.2 | CDS | 34051070 | 34051211 | 34051070 | ID=cds.MS.gene008905.t1;Parent=MS.gene008905.t1 |
| chr8.2 | exon | 34052072 | 34052152 | 34052072 | ID=MS.gene008905.t1.exon6;Parent=MS.gene008905.t1 |
| chr8.2 | CDS | 34052072 | 34052152 | 34052072 | ID=cds.MS.gene008905.t1;Parent=MS.gene008905.t1 |
| chr8.2 | exon | 34052684 | 34052812 | 34052684 | ID=MS.gene008905.t1.exon7;Parent=MS.gene008905.t1 |
| chr8.2 | CDS | 34052684 | 34052812 | 34052684 | ID=cds.MS.gene008905.t1;Parent=MS.gene008905.t1 |
| chr8.2 | exon | 34052924 | 34053001 | 34052924 | ID=MS.gene008905.t1.exon8;Parent=MS.gene008905.t1 |
| chr8.2 | CDS | 34052924 | 34053001 | 34052924 | ID=cds.MS.gene008905.t1;Parent=MS.gene008905.t1 |
| chr8.2 | exon | 34053108 | 34053179 | 34053108 | ID=MS.gene008905.t1.exon9;Parent=MS.gene008905.t1 |
| chr8.2 | CDS | 34053108 | 34053179 | 34053108 | ID=cds.MS.gene008905.t1;Parent=MS.gene008905.t1 |
| chr8.2 | exon | 34056014 | 34056094 | 34056014 | ID=MS.gene008905.t1.exon10;Parent=MS.gene008905.t1 |
| chr8.2 | CDS | 34056014 | 34056094 | 34056014 | ID=cds.MS.gene008905.t1;Parent=MS.gene008905.t1 |
| chr8.2 | exon | 34056327 | 34056377 | 34056327 | ID=MS.gene008905.t1.exon11;Parent=MS.gene008905.t1 |
| chr8.2 | CDS | 34056327 | 34056377 | 34056327 | ID=cds.MS.gene008905.t1;Parent=MS.gene008905.t1 |
| Gene Sequence |
| Protein sequence |
