AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene009435

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene009435.t1 MTR_3g008640 96.639 476 16 0 1 476 52 527 0.0 971
MS.gene009435.t1 MTR_3g008720 96.429 476 17 0 1 476 52 527 0.0 971
MS.gene009435.t1 MTR_3g008700 96.429 476 17 0 1 476 52 527 0.0 970
MS.gene009435.t1 MTR_3g033690 94.118 476 27 1 1 476 52 526 0.0 947
MS.gene009435.t1 MTR_7g053360 94.958 476 24 0 1 476 52 527 0.0 939
MS.gene009435.t1 MTR_4g035980 66.876 477 150 3 7 476 64 539 0.0 672
MS.gene009435.t1 MTR_4g025690 54.430 474 202 9 4 476 53 513 1.85e-173 497
MS.gene009435.t1 MTR_4g025730 49.585 482 216 12 2 476 48 509 4.10e-157 456
MS.gene009435.t1 MTR_8g104655 45.621 491 238 6 7 476 59 541 1.92e-151 442
MS.gene009435.t1 MTR_7g108905 48.190 442 204 8 51 472 116 552 8.59e-143 421
MS.gene009435.t1 MTR_8g104645 55.848 342 146 2 139 476 32 372 7.59e-139 404
MS.gene009435.t1 MTR_7g050980 48.889 450 207 11 41 476 115 555 7.68e-137 405
MS.gene009435.t1 MTR_7g050870 47.577 454 215 10 37 476 110 554 1.18e-134 400
MS.gene009435.t1 MTR_7g050950 50.608 411 184 10 78 476 150 553 6.35e-134 398
MS.gene009435.t1 MTR_4g087980 47.696 434 213 6 51 476 89 516 1.17e-131 391
MS.gene009435.t1 MTR_3g091380 45.000 460 220 10 46 476 142 597 4.31e-130 390
MS.gene009435.t1 MTR_2g044880 47.573 412 209 6 68 476 118 525 1.82e-129 385
MS.gene009435.t1 MTR_4g025670 45.602 432 219 7 51 476 68 489 9.54e-129 382
MS.gene009435.t1 MTR_4g087970 45.249 442 228 6 45 476 80 517 1.95e-128 383
MS.gene009435.t1 MTR_8g023310 45.815 454 215 8 51 476 139 589 5.47e-127 381
MS.gene009435.t1 MTR_8g104580 41.344 491 226 8 7 476 51 500 1.98e-125 374
MS.gene009435.t1 MTR_1g008140 42.612 467 228 9 46 476 145 607 7.76e-123 371
MS.gene009435.t1 MTR_7g112580 44.494 445 217 8 51 476 109 542 9.37e-122 366
MS.gene009435.t1 MTR_1g083520 44.586 471 243 10 22 476 60 528 1.70e-120 363
MS.gene009435.t1 MTR_7g108900 42.398 467 224 9 51 476 98 560 1.71e-120 364
MS.gene009435.t1 MTR_2g044810 42.956 433 232 8 51 476 110 534 7.79e-120 361
MS.gene009435.t1 MTR_8g042900 42.887 485 245 11 15 476 106 581 5.35e-119 360
MS.gene009435.t1 MTR_1g054880 43.413 463 222 9 51 476 99 558 8.14e-118 357
MS.gene009435.t1 MTR_4g124520 42.824 425 227 7 61 476 104 521 1.31e-117 355
MS.gene009435.t1 MTR_3g010780 42.650 483 247 12 15 476 101 574 2.81e-116 353
MS.gene009435.t1 MTR_8g042910 51.051 333 156 4 149 476 282 612 6.70e-116 353
MS.gene009435.t1 MTR_1g088020 42.735 468 220 10 51 476 94 555 1.03e-115 351
MS.gene009435.t1 MTR_7g108875 39.920 501 255 11 19 476 67 564 1.85e-112 343
MS.gene009435.t1 MTR_3g010770 52.698 315 145 3 164 476 263 575 2.95e-112 343
MS.gene009435.t1 MTR_7g108890 50.751 333 157 4 149 476 232 562 4.42e-112 342
MS.gene009435.t1 MTR_1g088225 42.094 468 221 11 51 476 94 553 6.01e-110 337
MS.gene009435.t1 MTR_0458s0030 57.194 278 113 4 202 476 40 314 6.47e-109 325
MS.gene009435.t1 MTR_1g088040 57.194 278 113 4 202 476 40 314 6.47e-109 325
MS.gene009435.t1 MTR_4g124550 39.957 463 243 8 41 476 139 593 1.52e-105 326
MS.gene009435.t1 MTR_8g023150 40.166 483 262 9 15 476 106 582 5.55e-105 325
MS.gene009435.t1 MTR_0618s0020 61.842 228 86 1 249 476 2 228 4.38e-103 307
MS.gene009435.t1 MTR_1g083470 38.462 442 248 7 52 472 128 566 6.13e-101 314
MS.gene009435.t1 MTR_1g083500 38.462 442 248 7 52 472 128 566 6.13e-101 314
MS.gene009435.t1 MTR_4g005170 39.052 443 244 8 52 472 133 571 1.41e-99 312
MS.gene009435.t1 MTR_1g083490 38.600 443 246 8 52 472 133 571 6.77e-99 309
MS.gene009435.t1 MTR_4g005180 37.849 465 262 9 30 472 94 553 3.45e-98 306
MS.gene009435.t1 MTR_1g083510 37.716 464 244 13 52 475 124 582 4.95e-98 306
MS.gene009435.t1 MTR_1g086210 43.391 348 188 5 136 476 224 569 4.08e-94 296
MS.gene009435.t1 MTR_1g086190 38.608 474 257 11 30 476 97 563 1.10e-92 292
MS.gene009435.t1 MTR_4g120290 35.021 474 274 8 27 475 107 571 1.33e-92 292
MS.gene009435.t1 MTR_2g044810 51.575 254 121 2 223 476 214 465 3.98e-92 288
MS.gene009435.t1 MTR_4g120280 37.232 419 235 6 81 475 157 571 2.78e-91 289
MS.gene009435.t1 MTR_3g095920 38.105 475 238 14 31 465 95 553 7.81e-91 287
MS.gene009435.t1 MTR_8g093460 37.708 480 246 15 22 468 84 543 2.59e-90 286
MS.gene009435.t1 MTR_2g436040 37.838 407 233 8 81 473 143 543 3.61e-90 287
MS.gene009435.t1 MTR_7g112590 36.797 462 248 11 52 475 127 582 5.60e-90 286
MS.gene009435.t1 MTR_8g088610 41.322 363 195 5 123 473 17 373 9.68e-90 278
MS.gene009435.t1 MTR_2g044660 46.497 314 161 5 163 476 326 632 1.78e-88 283
MS.gene009435.t1 MTR_7g110440 35.381 472 281 10 17 476 115 574 8.96e-86 275
MS.gene009435.t1 MTR_7g110490 39.340 394 223 6 89 471 181 569 2.41e-85 273
MS.gene009435.t1 MTR_7g110510 39.340 394 223 6 89 471 181 569 2.41e-85 273
MS.gene009435.t1 MTR_2g436050 36.957 414 238 7 81 475 154 563 1.27e-83 268
MS.gene009435.t1 MTR_5g083620 39.045 356 205 7 127 476 7 356 1.84e-81 256
MS.gene009435.t1 MTR_7g110560 40.351 342 195 4 140 476 212 549 9.03e-78 253
MS.gene009435.t1 MTR_7g110420 38.889 360 197 6 139 476 228 586 1.39e-75 248
MS.gene009435.t1 MTR_2g105560 40.317 315 172 5 163 476 40 339 2.15e-74 238
MS.gene009435.t1 MTR_7g107180 40.446 314 173 6 164 476 7 307 3.98e-72 231
MS.gene009435.t1 MTR_7g110430 33.500 400 214 9 89 476 181 540 2.83e-64 217
MS.gene009435.t1 MTR_7g111560 33.108 444 267 14 51 476 125 556 2.27e-60 207
MS.gene009435.t1 MTR_2g039530 38.623 334 181 9 134 460 23 339 1.81e-56 191
MS.gene009435.t1 MTR_6g012800 34.641 306 174 7 164 459 6 295 3.54e-56 189
MS.gene009435.t1 MTR_0458s0010 32.051 468 233 14 51 476 94 518 2.30e-55 193
MS.gene009435.t1 MTR_6g024165 37.621 311 166 9 166 466 43 335 2.24e-54 185
MS.gene009435.t1 MTR_6g088810 35.057 348 191 11 136 465 47 377 2.39e-54 186
MS.gene009435.t1 MTR_1g086540 37.224 317 172 8 161 467 47 346 8.74e-54 184
MS.gene009435.t1 MTR_8g102380 35.629 334 179 12 146 465 66 377 3.45e-53 183
MS.gene009435.t1 MTR_7g011530 34.035 285 161 6 166 438 8 277 4.69e-49 171
MS.gene009435.t1 MTR_7g102600 31.165 369 217 11 105 465 54 393 2.38e-47 168
MS.gene009435.t1 MTR_8g102380 35.943 281 148 9 146 412 66 328 9.33e-45 160
MS.gene009435.t1 MTR_5g083590 31.746 315 162 5 163 476 6 268 4.10e-43 154
MS.gene009435.t1 MTR_5g095490 33.766 308 175 9 170 465 67 357 5.12e-43 155
MS.gene009435.t1 MTR_6g042660 33.808 281 159 9 164 438 37 296 5.94e-43 155
MS.gene009435.t1 MTR_7g092780 32.803 314 187 7 164 467 90 389 1.10e-42 155
MS.gene009435.t1 MTR_5g093970 32.673 303 189 5 164 466 43 330 1.91e-42 154
MS.gene009435.t1 MTR_4g124740 31.692 325 187 10 150 465 41 339 7.83e-41 149
MS.gene009435.t1 MTR_1g033050 33.455 275 159 7 172 438 26 284 1.63e-40 148
MS.gene009435.t1 MTR_5g012600 30.380 316 193 8 164 468 63 362 4.98e-40 148
MS.gene009435.t1 MTR_6g042690 33.099 284 162 8 164 438 39 303 1.43e-37 140
MS.gene009435.t1 MTR_2g033970 29.878 328 201 8 147 465 39 346 1.97e-37 140
MS.gene009435.t1 MTR_5g088300 33.091 275 165 6 164 438 37 292 7.39e-37 138
MS.gene009435.t1 MTR_5g088280 31.900 279 171 6 160 438 35 294 2.97e-35 134
MS.gene009435.t1 MTR_2g073390 29.706 340 200 10 145 465 42 361 3.10e-35 135
MS.gene009435.t1 MTR_4g074290 28.783 337 209 9 144 465 41 361 2.74e-34 132
MS.gene009435.t1 MTR_4g051810 30.844 308 190 7 160 466 33 318 4.57e-34 130
MS.gene009435.t1 MTR_5g088290 31.636 275 170 5 164 438 48 304 2.52e-33 129
MS.gene009435.t1 MTR_0009s0350 29.204 339 205 10 144 465 41 361 3.67e-32 126
MS.gene009435.t1 MTR_0009s0340 29.204 339 205 10 144 465 41 361 3.67e-32 126
MS.gene009435.t1 MTR_2g436600 28.267 329 196 10 147 465 38 336 1.98e-30 121
MS.gene009435.t1 MTR_8g036225 30.033 303 180 11 146 438 18 298 1.54e-29 118
MS.gene009435.t1 MTR_6g025590 37.126 167 96 5 166 324 51 216 1.75e-25 105
MS.gene009435.t1 MTR_0408s0030 40.594 101 50 2 338 438 33 123 4.56e-18 81.6
MS.gene009435.t1 MTR_4g130790 31.720 186 113 5 170 346 30 210 2.10e-17 82.4
MS.gene009435.t1 MTR_6g024200 37.600 125 66 3 341 465 164 276 3.36e-16 79.3
MS.gene009435.t1 MTR_2g436070 31.034 174 96 5 81 236 154 321 3.13e-14 74.7
MS.gene009435.t1 MTR_7g102570 32.174 115 65 3 324 436 3 106 1.14e-11 62.4
MS.gene009435.t1 MTR_7g102580 40.698 86 41 3 239 319 73 153 1.89e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene009435.t1 AT3G47400 57.588 481 191 7 8 476 115 594 0.0 555
MS.gene009435.t1 AT5G51490 56.303 476 193 7 12 476 65 536 0.0 553
MS.gene009435.t1 AT5G51500 56.108 483 198 10 5 476 61 540 0.0 548
MS.gene009435.t1 AT2G45220 50.630 476 219 10 2 476 51 511 1.64e-159 462
MS.gene009435.t1 AT4G00190 44.915 472 239 9 7 476 22 474 1.50e-135 399
MS.gene009435.t1 AT1G23200 44.135 503 226 10 13 476 68 554 5.62e-135 400
MS.gene009435.t1 AT1G11580 46.882 465 230 8 22 476 100 557 6.26e-134 398
MS.gene009435.t1 AT1G11580 46.882 465 230 8 22 476 121 578 1.30e-133 398
MS.gene009435.t1 AT5G53370 45.354 452 222 7 46 476 138 585 1.06e-129 388
MS.gene009435.t1 AT3G60730 42.363 491 248 8 15 476 35 519 2.22e-129 385
MS.gene009435.t1 AT5G53370 45.354 452 222 7 46 476 174 621 4.98e-129 388
MS.gene009435.t1 AT3G14310 45.570 474 212 8 45 476 123 592 2.55e-127 382
MS.gene009435.t1 AT4G02320 44.866 448 216 8 51 476 80 518 1.87e-126 378
MS.gene009435.t1 AT3G59010 44.421 475 232 8 18 476 70 528 2.86e-126 377
MS.gene009435.t1 AT3G43270 44.843 446 223 7 51 476 83 525 1.78e-125 375
MS.gene009435.t1 AT4G02300 44.470 443 223 9 51 476 96 532 7.61e-125 374
MS.gene009435.t1 AT1G53830 45.870 460 211 8 51 476 132 587 1.00e-124 375
MS.gene009435.t1 AT3G49220 44.469 452 224 8 46 476 151 596 1.30e-124 375
MS.gene009435.t1 AT3G49220 44.469 452 224 8 46 476 196 641 6.74e-124 375
MS.gene009435.t1 AT4G33220 42.888 457 226 7 51 476 71 523 8.34e-122 366
MS.gene009435.t1 AT4G33220 50.578 346 168 3 131 476 60 402 2.28e-120 358
MS.gene009435.t1 AT2G43050 45.183 436 210 8 51 476 100 516 2.57e-120 362
MS.gene009435.t1 AT5G20860 43.864 440 229 9 47 476 107 538 2.66e-120 362
MS.gene009435.t1 AT5G04960 43.125 480 239 11 15 474 97 562 9.56e-120 362
MS.gene009435.t1 AT2G47550 53.134 335 150 4 147 476 226 558 1.80e-116 353
MS.gene009435.t1 AT4G02330 48.468 359 169 5 125 476 222 571 2.38e-116 353
MS.gene009435.t1 AT3G05620 45.658 403 197 8 83 476 152 541 5.45e-116 352
MS.gene009435.t1 AT1G02810 40.741 486 225 10 51 476 95 577 1.50e-115 352
MS.gene009435.t1 AT2G26450 39.752 483 264 8 7 476 143 611 1.79e-115 353
MS.gene009435.t1 AT5G49180 40.851 470 251 9 27 472 100 566 2.74e-114 348
MS.gene009435.t1 AT2G26440 41.648 449 231 9 51 476 107 547 3.02e-113 345
MS.gene009435.t1 AT3G10710 44.009 434 226 9 52 476 136 561 1.36e-111 341
MS.gene009435.t1 AT5G27870 38.174 482 278 8 11 476 85 562 2.54e-110 343
MS.gene009435.t1 AT3G05610 38.636 484 275 8 11 476 86 565 7.78e-110 340
MS.gene009435.t1 AT4G33230 38.866 476 257 10 22 476 144 606 8.62e-110 338
MS.gene009435.t1 AT3G27980 44.638 401 204 8 84 476 107 497 3.36e-109 333
MS.gene009435.t1 AT1G11590 40.909 484 255 14 14 476 51 524 8.20e-109 333
MS.gene009435.t1 AT3G06830 37.628 489 271 11 17 474 81 566 1.36e-108 333
MS.gene009435.t1 AT4G03930 39.787 470 240 11 19 476 86 524 6.22e-107 328
MS.gene009435.t1 AT3G10720 51.111 315 150 3 164 476 303 615 1.23e-105 327
MS.gene009435.t1 AT5G04970 47.962 319 162 3 160 476 304 620 5.80e-100 313
MS.gene009435.t1 AT3G10720 55.172 261 115 2 216 476 1 259 4.29e-99 298
MS.gene009435.t1 AT5G20860 40.811 419 202 8 47 455 107 489 4.45e-99 307
MS.gene009435.t1 AT1G53840 36.992 492 262 12 15 470 102 581 8.27e-97 303
MS.gene009435.t1 AT3G14300 38.155 477 260 10 5 461 492 953 3.60e-94 306
MS.gene009435.t1 AT4G15980 42.149 363 202 6 117 474 341 700 2.03e-91 293
MS.gene009435.t1 AT4G15980 42.149 363 202 6 117 474 351 710 3.58e-91 292
MS.gene009435.t1 AT5G09760 48.161 299 141 5 158 443 235 532 8.90e-86 274
MS.gene009435.t1 AT5G09760 48.161 299 141 5 158 443 261 558 1.71e-85 274
MS.gene009435.t1 AT1G11370 48.988 247 125 1 161 407 43 288 1.47e-82 257
MS.gene009435.t1 AT5G64640 37.825 423 218 12 81 468 189 601 1.36e-81 264
MS.gene009435.t1 AT2G47030 39.326 356 198 6 135 476 237 588 1.03e-74 246
MS.gene009435.t1 AT2G47040 38.244 353 203 6 135 476 247 595 1.59e-72 240
MS.gene009435.t1 AT3G62170 37.158 366 200 9 135 476 229 588 4.17e-69 231
MS.gene009435.t1 AT2G36710 33.856 319 188 8 161 470 86 390 3.35e-51 179
MS.gene009435.t1 AT2G36700 34.591 318 185 9 156 465 31 333 3.67e-51 177
MS.gene009435.t1 AT2G21610 33.887 301 177 7 166 465 53 332 5.54e-51 177
MS.gene009435.t1 AT2G21610 33.887 301 177 7 166 465 58 337 7.12e-51 177
MS.gene009435.t1 AT5G19730 31.215 362 204 11 127 465 42 381 1.52e-50 177
MS.gene009435.t1 AT3G29090 33.684 285 162 6 166 438 9 278 1.23e-48 169
MS.gene009435.t1 AT1G05310 33.333 315 180 7 164 465 91 388 5.97e-46 164
MS.gene009435.t1 AT3G17060 30.960 323 192 8 153 465 35 336 1.16e-42 154
MS.gene009435.t1 AT5G47500 29.697 330 201 9 149 466 48 358 2.47e-40 149
MS.gene009435.t1 AT5G07430 29.882 338 206 10 142 465 35 355 9.63e-39 144
MS.gene009435.t1 AT1G44980 41.270 189 107 3 143 328 54 241 1.14e-38 141
MS.gene009435.t1 AT5G55590 33.333 318 183 10 160 465 78 378 1.35e-37 142
MS.gene009435.t1 AT3G24130 31.818 286 169 7 164 438 34 304 2.12e-37 140
MS.gene009435.t1 AT5G55590 34.866 261 145 7 160 408 78 325 7.45e-37 138
MS.gene009435.t1 AT5G07420 29.794 339 205 10 142 465 35 355 1.17e-36 139
MS.gene009435.t1 AT5G18990 29.900 301 174 9 153 438 21 299 1.12e-32 127
MS.gene009435.t1 AT5G26810 29.592 294 169 8 164 438 34 308 3.62e-32 125
MS.gene009435.t1 AT5G61680 28.190 337 208 10 145 465 40 358 5.06e-32 126
MS.gene009435.t1 AT5G07410 28.657 335 207 11 145 465 39 355 1.11e-31 125
MS.gene009435.t1 AT1G69940 28.060 335 209 11 145 465 39 355 1.00e-30 122
MS.gene009435.t1 AT2G19150 26.970 330 205 9 156 472 33 339 7.68e-30 119
MS.gene009435.t1 AT2G19150 28.571 266 158 7 156 408 33 279 2.57e-27 111
MS.gene009435.t1 AT2G47280 27.397 292 180 8 160 438 31 303 1.00e-26 110
MS.gene009435.t1 AT3G49220 31.081 222 127 7 46 246 196 412 2.92e-24 105

Find 103 sgRNAs with CRISPR-Local

Find 141 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTCCACAATTGTCAAATT+AGG 0.096980 3.4:-3869267 MS.gene009435:CDS
TACAGACAATGTTTCATTTA+TGG 0.113744 3.4:-3869427 MS.gene009435:CDS
GCTCAAGATACATTGTATTA+TGG 0.153901 3.4:-3869094 MS.gene009435:CDS
GGCACTGTAGACTTCATATT+TGG 0.203147 3.4:-3869406 MS.gene009435:CDS
TTGCGAAAATGTTACAGTTT+TGG 0.235352 3.4:+3869365 None:intergenic
GCATCAAAGTTTACGGTTAC+TGG 0.240632 3.4:-3868986 MS.gene009435:CDS
TGTGAGGACCAGCAGTATTT+TGG 0.243797 3.4:+3869557 None:intergenic
ACCACAAGATTGTACTTTAC+TGG 0.268962 3.4:+3870111 None:intergenic
ATTCCACAATTGTCAAATTA+GGG 0.271376 3.4:-3869266 MS.gene009435:CDS
ACGGTTACTGGACTCCTTGC+TGG 0.289286 3.4:-3868974 MS.gene009435:CDS
CTCAAGATACATTGTATTAT+GGG 0.311973 3.4:-3869093 MS.gene009435:CDS
GGAATGAAATTCCAGTGTTT+TGG 0.313831 3.4:+3869284 None:intergenic
CATTTAAATTTAGGTATTGA+TGG 0.320933 3.4:-3869610 MS.gene009435:intron
TCAAACATAGAGTTGAATTC+AGG 0.331287 3.4:-3870934 MS.gene009435:CDS
GATCAAAGACCAGATGTAAA+TGG 0.333623 3.4:+3868923 None:intergenic
CATCTCAGCCGCACACTTGA+AGG 0.342128 3.4:-3870770 MS.gene009435:CDS
TATGGGGAGTATGAGAATTA+TGG 0.345818 3.4:-3869076 MS.gene009435:CDS
GTAAACTTTGATGCTTCCTT+AGG 0.347510 3.4:+3868995 None:intergenic
TAAGGAAGCATCAAAGTTTA+CGG 0.357836 3.4:-3868993 MS.gene009435:CDS
TTCTACCGGTGTGCTATATC+AGG 0.383983 3.4:-3869490 MS.gene009435:CDS
TCATGAACAGAAAACCAGTT+TGG 0.390060 3.4:+3870159 None:intergenic
AAGAAGAGTCATTTCCAAAC+TGG 0.395925 3.4:-3870173 MS.gene009435:CDS
CTGGTTCATCAACAGCAAAC+AGG 0.401229 3.4:-3869051 MS.gene009435:CDS
TGACAAGTACAAAACCTTCC+TGG 0.405163 3.4:-3869218 MS.gene009435:CDS
GAATGGATCACCTCGACCTT+GGG 0.406727 3.4:+3869306 None:intergenic
AGCCATGTTTGTGCATCATT+TGG 0.409326 3.4:+3870719 None:intergenic
TTTCATGAAATGCATATTGA+TGG 0.410824 3.4:+3870580 None:intergenic
GCAACAATATTCAAGAGTTA+TGG 0.414279 3.4:-3869185 MS.gene009435:CDS
AAACCTTCCTGGGCAGGCCT+TGG 0.419119 3.4:-3869207 MS.gene009435:CDS
CAACAGCAAACAGGGTGAAA+TGG 0.423776 3.4:-3869042 MS.gene009435:CDS
CCAATGTCATTGAGATGATA+AGG 0.424647 3.4:-3870613 MS.gene009435:CDS
GTGGTTGCGAAAGATGGTTC+TGG 0.428910 3.4:-3870093 MS.gene009435:CDS
TCAGTGCATAAAACGGTTCA+TGG 0.448897 3.4:-3870827 MS.gene009435:CDS
GGAATGGATCACCTCGACCT+TGG 0.452399 3.4:+3869305 None:intergenic
AATCTTGTGGTTGCGAAAGA+TGG 0.453128 3.4:-3870099 MS.gene009435:CDS
ACAAGCTCTAGAAGTGTTCA+AGG 0.465823 3.4:-3869904 MS.gene009435:CDS
CAGCCCTAATTTGACAATTG+TGG 0.474354 3.4:+3869263 None:intergenic
TCACCCCATGGAGACCAACC+TGG 0.478370 3.4:+3869127 None:intergenic
CTGATCTATCTGTCTTCTAC+CGG 0.482455 3.4:-3869504 MS.gene009435:CDS
ACTACTGTGCCATTTACATC+TGG 0.484988 3.4:-3868932 MS.gene009435:CDS
TAAACTTTGATGCTTCCTTA+GGG 0.485228 3.4:+3868996 None:intergenic
ATTGTACTTTACTGGAGACT+TGG 0.493638 3.4:+3870119 None:intergenic
AATATTGAAACATGTAAAAG+TGG 0.495425 3.4:-3870680 MS.gene009435:CDS
TTTGTGATACATGTTAAGAA+AGG 0.500212 3.4:-3870009 MS.gene009435:CDS
CCTTATCATCTCAATGACAT+TGG 0.503793 3.4:+3870613 None:intergenic
GGGCGGTGATCATGTTAGCT+TGG 0.512801 3.4:+3869326 None:intergenic
CAGATAGATCAGAGGCTGAT+CGG 0.521445 3.4:+3869515 None:intergenic
CAAACATAGAGTTGAATTCA+GGG 0.525299 3.4:-3870933 MS.gene009435:CDS
GACAAGTACAAAACCTTCCT+GGG 0.525686 3.4:-3869217 MS.gene009435:CDS
ATGATCACCGCCCAAGGTCG+AGG 0.526125 3.4:-3869316 MS.gene009435:CDS
CATGAGCCATGAGGGTGTCT+TGG 0.529891 3.4:+3869464 None:intergenic
CACCCCATGGAGACCAACCT+GGG 0.530544 3.4:+3869128 None:intergenic
CTGTTTGCTGTTGATGAACC+AGG 0.531874 3.4:+3869052 None:intergenic
GCAAACAGGGTGAAATGGCC+TGG 0.531958 3.4:-3869037 MS.gene009435:CDS
TTCGCAAGAAAGCCTTTGGA+TGG 0.536363 3.4:-3869349 MS.gene009435:CDS
ACTCTTGAATATTGTTGCCA+AGG 0.536658 3.4:+3869190 None:intergenic
ATTGTTGCCAAGGCCTGCCC+AGG 0.539912 3.4:+3869200 None:intergenic
ATGGACTTCACTTCATTGCA+CGG 0.543309 3.4:-3869591 MS.gene009435:CDS
TGCTGTTGCAGAGCTATAAG+TGG 0.543731 3.4:+3869873 None:intergenic
AACATTATGCTGGTTGGTGA+TGG 0.543858 3.4:-3869946 MS.gene009435:CDS
AGTACAAAACCTTCCTGGGC+AGG 0.545921 3.4:-3869213 MS.gene009435:CDS
AAACTCATCAGTGCATAAAA+CGG 0.551962 3.4:-3870834 MS.gene009435:CDS
ACTTATAGCTCTGCAACAGC+AGG 0.557059 3.4:-3869871 MS.gene009435:intron
TCCTCACAAGGGTCAAGCTG+TGG 0.557729 3.4:-3869542 MS.gene009435:CDS
AAATACTGCTGGTCCTCACA+AGG 0.561048 3.4:-3869554 MS.gene009435:CDS
CACCAAATGATGCACAAACA+TGG 0.565228 3.4:-3870721 MS.gene009435:CDS
ATAACCTTCCAAAATACTGC+TGG 0.566373 3.4:-3869565 MS.gene009435:CDS
CTAAGAGAAAATACAGGACA+AGG 0.578008 3.4:-3870032 MS.gene009435:CDS
TGGACTTCACTTCATTGCAC+GGG 0.579713 3.4:-3869590 MS.gene009435:CDS
GGTGATCCATTCCAAAACAC+TGG 0.579879 3.4:-3869295 MS.gene009435:CDS
TCATAATGATAACATTATGC+TGG 0.579993 3.4:-3869956 MS.gene009435:CDS
TTGCCAAGGCCTGCCCAGGA+AGG 0.582257 3.4:+3869204 None:intergenic
TCATGTGATAACAAACCCTA+AGG 0.582871 3.4:-3869011 MS.gene009435:CDS
ATGTTAGCTTGGCCATCCAA+AGG 0.583281 3.4:+3869337 None:intergenic
AATGATAACATTATGCTGGT+TGG 0.589997 3.4:-3869952 MS.gene009435:CDS
CCTTGCTGGACCAACATGGT+TGG 0.591137 3.4:-3868960 MS.gene009435:CDS
TGGATCACCTCGACCTTGGG+CGG 0.595949 3.4:+3869309 None:intergenic
TCAAGATACATTGTATTATG+GGG 0.596259 3.4:-3869092 MS.gene009435:CDS
TCCAGTAAAGTACAATCTTG+TGG 0.603808 3.4:-3870112 MS.gene009435:CDS
TGTTTAAGCCTTCAAGTGTG+CGG 0.604466 3.4:+3870762 None:intergenic
GCCACAGCTTGACCCTTGTG+AGG 0.610896 3.4:+3869541 None:intergenic
GCTAACATGATCACCGCCCA+AGG 0.613648 3.4:-3869322 MS.gene009435:CDS
TGTTTATAGAGAGAACATTG+AGG 0.615615 3.4:-3869986 MS.gene009435:CDS
TGGTTCATCAACAGCAAACA+GGG 0.617491 3.4:-3869050 MS.gene009435:CDS
TTTGTTATCACATGATAACC+AGG 0.617495 3.4:+3869019 None:intergenic
GCAAAATCAGTGTCACCCCA+TGG 0.619580 3.4:+3869115 None:intergenic
GACTCCTTGCTGGACCAACA+TGG 0.622054 3.4:-3868964 MS.gene009435:CDS
CTGCTGCTAAGAGAAAATAC+AGG 0.626019 3.4:-3870038 MS.gene009435:CDS
ACCCCATGGAGACCAACCTG+GGG 0.627096 3.4:+3869129 None:intergenic
CCAACCATGTTGGTCCAGCA+AGG 0.628774 3.4:+3868960 None:intergenic
AGGCTACCAAGACACCCTCA+TGG 0.632122 3.4:-3869470 MS.gene009435:CDS
TGCATCAGATCTAAAGCCTG+TGG 0.645806 3.4:-3869242 MS.gene009435:CDS
CCCCATGGAGACCAACCTGG+GGG 0.656474 3.4:+3869130 None:intergenic
GAGTATGAGAATTATGGACC+TGG 0.666021 3.4:-3869070 MS.gene009435:CDS
GTAGAAGACAGATAGATCAG+AGG 0.666155 3.4:+3869507 None:intergenic
ACGTTGTGCATGAGCCATGA+GGG 0.666275 3.4:+3869456 None:intergenic
GTAGTTGTAGCCAACCATGT+TGG 0.667448 3.4:+3868950 None:intergenic
CCCATGGAGACCAACCTGGG+GGG 0.685029 3.4:+3869131 None:intergenic
AATACTGCTGGTCCTCACAA+GGG 0.685253 3.4:-3869553 MS.gene009435:CDS
CATTTCATGAAACATGACAA+AGG 0.696167 3.4:-3870569 MS.gene009435:intron
GACGTTGTGCATGAGCCATG+AGG 0.724421 3.4:+3869455 None:intergenic
AGCTCTAGAAGTGTTCAAGG+TGG 0.733912 3.4:-3869901 MS.gene009435:CDS
GGTAGCCTGATATAGCACAC+CGG 0.754487 3.4:+3869485 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGATATAAAATAATTTCTT+TGG - chr3.4:3870118-3870137 MS.gene009435:CDS 10.0%
!! ATATTTATTATTATACTTTG+CGG - chr3.4:3870196-3870215 MS.gene009435:intron 10.0%
!! TAAATTATTGAATGTTTATT+TGG - chr3.4:3869619-3869638 MS.gene009435:intron 10.0%
!!! ATAATTCAATAACTTTATTT+AGG - chr3.4:3869579-3869598 MS.gene009435:CDS 10.0%
!!! CTAAAATTTTATAATAAGAT+AGG - chr3.4:3870167-3870186 MS.gene009435:CDS 10.0%
!!! AGTACAATACTTAAATTTTA+AGG + chr3.4:3869551-3869570 None:intergenic 15.0%
!!! GTACAATACTTAAATTTTAA+GGG + chr3.4:3869550-3869569 None:intergenic 15.0%
!!! TACAATACTTAAATTTTAAG+GGG + chr3.4:3869549-3869568 None:intergenic 15.0%
!!! TTTTTTAAAATAATCACTCT+TGG - chr3.4:3870243-3870262 MS.gene009435:intron 15.0%
!! AATATTGAAACATGTAAAAG+TGG - chr3.4:3869198-3869217 MS.gene009435:CDS 20.0%
!! ACAAGAAAATAAAAAGAGAA+AGG + chr3.4:3870050-3870069 None:intergenic 20.0%
!!! ATTCAATAACTTTATTTAGG+AGG - chr3.4:3869582-3869601 MS.gene009435:CDS 20.0%
!!! CATTTAAATTTAGGTATTGA+TGG - chr3.4:3870268-3870287 MS.gene009435:intron 20.0%
! ATTCCACAATTGTCAAATTA+GGG - chr3.4:3870612-3870631 MS.gene009435:CDS 25.0%
! CAAGACATAATTTCTACTAA+TGG + chr3.4:3869419-3869438 None:intergenic 25.0%
! CATTAGTAGAAATTATGTCT+TGG - chr3.4:3869417-3869436 MS.gene009435:CDS 25.0%
! CTCAAGATACATTGTATTAT+GGG - chr3.4:3870785-3870804 MS.gene009435:CDS 25.0%
! CTCTTGGATCATTTAAATTT+AGG - chr3.4:3870259-3870278 MS.gene009435:intron 25.0%
! TACAGACAATGTTTCATTTA+TGG - chr3.4:3870451-3870470 MS.gene009435:intron 25.0%
! TCAAGATACATTGTATTATG+GGG - chr3.4:3870786-3870805 MS.gene009435:CDS 25.0%
! TCATAATGATAACATTATGC+TGG - chr3.4:3869922-3869941 MS.gene009435:CDS 25.0%
! TTTCATGAAATGCATATTGA+TGG + chr3.4:3869301-3869320 None:intergenic 25.0%
! TTTGTGATACATGTTAAGAA+AGG - chr3.4:3869869-3869888 MS.gene009435:intron 25.0%
!!! TATGGTGATGAAAACTTTTA+TGG - chr3.4:3870711-3870730 MS.gene009435:CDS 25.0%
AAACTCATCAGTGCATAAAA+CGG - chr3.4:3869044-3869063 MS.gene009435:CDS 30.0%
AATGATAACATTATGCTGGT+TGG - chr3.4:3869926-3869945 MS.gene009435:CDS 30.0%
CAAACATAGAGTTGAATTCA+GGG - chr3.4:3868945-3868964 MS.gene009435:CDS 30.0%
CATTCCACAATTGTCAAATT+AGG - chr3.4:3870611-3870630 MS.gene009435:CDS 30.0%
CATTTCATGAAACATGACAA+AGG - chr3.4:3869309-3869328 MS.gene009435:CDS 30.0%
GCAACAATATTCAAGAGTTA+TGG - chr3.4:3870693-3870712 MS.gene009435:CDS 30.0%
GCTCAAGATACATTGTATTA+TGG - chr3.4:3870784-3870803 MS.gene009435:CDS 30.0%
TAAACTTTGATGCTTCCTTA+GGG + chr3.4:3870885-3870904 None:intergenic 30.0%
TCAAACATAGAGTTGAATTC+AGG - chr3.4:3868944-3868963 MS.gene009435:CDS 30.0%
TGTTTATAGAGAGAACATTG+AGG - chr3.4:3869892-3869911 MS.gene009435:CDS 30.0%
! TTGCGAAAATGTTACAGTTT+TGG + chr3.4:3870516-3870535 None:intergenic 30.0%
! TTTGTTATCACATGATAACC+AGG + chr3.4:3870862-3870881 None:intergenic 30.0%
!! TAAGGAAGCATCAAAGTTTA+CGG - chr3.4:3870885-3870904 MS.gene009435:CDS 30.0%
AAGAAGAGTCATTTCCAAAC+TGG - chr3.4:3869705-3869724 MS.gene009435:intron 35.0%
ACCACAAGATTGTACTTTAC+TGG + chr3.4:3869770-3869789 None:intergenic 35.0%
ACTCTTGAATATTGTTGCCA+AGG + chr3.4:3870691-3870710 None:intergenic 35.0%
ATAACCTTCCAAAATACTGC+TGG - chr3.4:3870313-3870332 MS.gene009435:intron 35.0%
ATTGTACTTTACTGGAGACT+TGG + chr3.4:3869762-3869781 None:intergenic 35.0%
CCAATGTCATTGAGATGATA+AGG - chr3.4:3869265-3869284 MS.gene009435:CDS 35.0%
CCTTATCATCTCAATGACAT+TGG + chr3.4:3869268-3869287 None:intergenic 35.0%
CTAAGAGAAAATACAGGACA+AGG - chr3.4:3869846-3869865 MS.gene009435:intron 35.0%
GTAAACTTTGATGCTTCCTT+AGG + chr3.4:3870886-3870905 None:intergenic 35.0%
TACTTTAACCAATGCCTCAA+GGG - chr3.4:3869509-3869528 MS.gene009435:CDS 35.0%
TCATGAACAGAAAACCAGTT+TGG + chr3.4:3869722-3869741 None:intergenic 35.0%
TCATGTGATAACAAACCCTA+AGG - chr3.4:3870867-3870886 MS.gene009435:CDS 35.0%
TCCAGTAAAGTACAATCTTG+TGG - chr3.4:3869766-3869785 MS.gene009435:intron 35.0%
! GGAATGAAATTCCAGTGTTT+TGG + chr3.4:3870597-3870616 None:intergenic 35.0%
! TATGGGGAGTATGAGAATTA+TGG - chr3.4:3870802-3870821 MS.gene009435:CDS 35.0%
! TTCTTGTGCTTCTTTTTGCA+TGG + chr3.4:3869007-3869026 None:intergenic 35.0%
!! TATGCACTGATGAGTTTTGT+TGG + chr3.4:3869041-3869060 None:intergenic 35.0%
!!! ACAGTTTTGGAATACAACTG+CGG + chr3.4:3870503-3870522 None:intergenic 35.0%
!!! ACTGGTTTTCTGTTCATGAA+AGG - chr3.4:3869723-3869742 MS.gene009435:intron 35.0%
!!! CATGGTAAGAGCTTGTTTTA+AGG + chr3.4:3868989-3869008 None:intergenic 35.0%
!!! GAAATTCCAGTGTTTTGGAA+TGG + chr3.4:3870592-3870611 None:intergenic 35.0%
AATCTTGTGGTTGCGAAAGA+TGG - chr3.4:3869779-3869798 MS.gene009435:intron 40.0%
AGCCATGTTTGTGCATCATT+TGG + chr3.4:3869162-3869181 None:intergenic 40.0%
ATGGACTTCACTTCATTGCA+CGG - chr3.4:3870287-3870306 MS.gene009435:intron 40.0%
CACCAAATGATGCACAAACA+TGG - chr3.4:3869157-3869176 MS.gene009435:CDS 40.0%
CAGCCCTAATTTGACAATTG+TGG + chr3.4:3870618-3870637 None:intergenic 40.0%
CTGATCTATCTGTCTTCTAC+CGG - chr3.4:3870374-3870393 MS.gene009435:intron 40.0%
CTGCTGCTAAGAGAAAATAC+AGG - chr3.4:3869840-3869859 MS.gene009435:intron 40.0%
GACAAGTACAAAACCTTCCT+GGG - chr3.4:3870661-3870680 MS.gene009435:CDS 40.0%
GAGTATGAGAATTATGGACC+TGG - chr3.4:3870808-3870827 MS.gene009435:CDS 40.0%
GCATCAAAGTTTACGGTTAC+TGG - chr3.4:3870892-3870911 MS.gene009435:CDS 40.0%
GGCACTGTAGACTTCATATT+TGG - chr3.4:3870472-3870491 MS.gene009435:intron 40.0%
GTACTTTAACCAATGCCTCA+AGG - chr3.4:3869508-3869527 MS.gene009435:CDS 40.0%
GTAGAAGACAGATAGATCAG+AGG + chr3.4:3870374-3870393 None:intergenic 40.0%
TCAGTGCATAAAACGGTTCA+TGG - chr3.4:3869051-3869070 MS.gene009435:CDS 40.0%
TGACAAGTACAAAACCTTCC+TGG - chr3.4:3870660-3870679 MS.gene009435:CDS 40.0%
TGGTTCATCAACAGCAAACA+GGG - chr3.4:3870828-3870847 MS.gene009435:CDS 40.0%
TGTTTAAGCCTTCAAGTGTG+CGG + chr3.4:3869119-3869138 None:intergenic 40.0%
! CATTTTCGCAAGAAAGCCTT+TGG - chr3.4:3870525-3870544 MS.gene009435:intron 40.0%
! GTTTTGTACTTGTCAACCAC+AGG + chr3.4:3870655-3870674 None:intergenic 40.0%
!! AACATTATGCTGGTTGGTGA+TGG - chr3.4:3869932-3869951 MS.gene009435:CDS 40.0%
!! ACAAGCTCTAGAAGTGTTCA+AGG - chr3.4:3869974-3869993 MS.gene009435:CDS 40.0%
!! ACTACTGTGCCATTTACATC+TGG - chr3.4:3870946-3870965 MS.gene009435:CDS 40.0%
AAATACTGCTGGTCCTCACA+AGG - chr3.4:3870324-3870343 MS.gene009435:intron 45.0%
AATACTGCTGGTCCTCACAA+GGG - chr3.4:3870325-3870344 MS.gene009435:intron 45.0%
AATGCTAATGAGTGCCCTTG+AGG + chr3.4:3869526-3869545 None:intergenic 45.0%
CAACAGCAAACAGGGTGAAA+TGG - chr3.4:3870836-3870855 MS.gene009435:CDS 45.0%
CAGATAGATCAGAGGCTGAT+CGG + chr3.4:3870366-3870385 None:intergenic 45.0%
CTGGTTCATCAACAGCAAAC+AGG - chr3.4:3870827-3870846 MS.gene009435:CDS 45.0%
GGTGATCCATTCCAAAACAC+TGG - chr3.4:3870583-3870602 MS.gene009435:CDS 45.0%
GTAGTTGTAGCCAACCATGT+TGG + chr3.4:3870931-3870950 None:intergenic 45.0%
TGCTGTTGCAGAGCTATAAG+TGG + chr3.4:3870008-3870027 None:intergenic 45.0%
TGGACTTCACTTCATTGCAC+GGG - chr3.4:3870288-3870307 MS.gene009435:intron 45.0%
TTCGCAAGAAAGCCTTTGGA+TGG - chr3.4:3870529-3870548 MS.gene009435:intron 45.0%
TTCTACCGGTGTGCTATATC+AGG - chr3.4:3870388-3870407 MS.gene009435:intron 45.0%
! ACTTATAGCTCTGCAACAGC+AGG - chr3.4:3870007-3870026 MS.gene009435:CDS 45.0%
! ATGTTAGCTTGGCCATCCAA+AGG + chr3.4:3870544-3870563 None:intergenic 45.0%
! TGCATCAGATCTAAAGCCTG+TGG - chr3.4:3870636-3870655 MS.gene009435:CDS 45.0%
! TGTGAGGACCAGCAGTATTT+TGG + chr3.4:3870324-3870343 None:intergenic 45.0%
!! AGCTCTAGAAGTGTTCAAGG+TGG - chr3.4:3869977-3869996 MS.gene009435:CDS 45.0%
!! AGGACCAGCAGTATTTTGGA+AGG + chr3.4:3870320-3870339 None:intergenic 45.0%
!! CTGTTTGCTGTTGATGAACC+AGG + chr3.4:3870829-3870848 None:intergenic 45.0%
!!! ATATTTTTCTATTTAAAATT+TGG - chr3.4:3869348-3869367 MS.gene009435:CDS 5.0%
!!! TATTTTTCTATTTAAAATTT+GGG - chr3.4:3869349-3869368 MS.gene009435:CDS 5.0%
AATGAGTGCCCTTGAGGCAT+TGG + chr3.4:3869520-3869539 None:intergenic 50.0%
ACGTTGTGCATGAGCCATGA+GGG + chr3.4:3870425-3870444 None:intergenic 50.0%
AGTACAAAACCTTCCTGGGC+AGG - chr3.4:3870665-3870684 MS.gene009435:CDS 50.0%
CTTGCTAACCAGTGCTCTGA+GGG - chr3.4:3869373-3869392 MS.gene009435:CDS 50.0%
GAATGGATCACCTCGACCTT+GGG + chr3.4:3870575-3870594 None:intergenic 50.0%
GCAAAATCAGTGTCACCCCA+TGG + chr3.4:3870766-3870785 None:intergenic 50.0%
GGTAGCCTGATATAGCACAC+CGG + chr3.4:3870396-3870415 None:intergenic 50.0%
GTGGTTGCGAAAGATGGTTC+TGG - chr3.4:3869785-3869804 MS.gene009435:intron 50.0%
TCTTGCTAACCAGTGCTCTG+AGG - chr3.4:3869372-3869391 MS.gene009435:CDS 50.0%
AACCATTGCCCTCAGAGCAC+TGG + chr3.4:3869384-3869403 None:intergenic 55.0%
ACGGTTACTGGACTCCTTGC+TGG - chr3.4:3870904-3870923 MS.gene009435:CDS 55.0%
AGGCTACCAAGACACCCTCA+TGG - chr3.4:3870408-3870427 MS.gene009435:intron 55.0%
CATCTCAGCCGCACACTTGA+AGG - chr3.4:3869108-3869127 MS.gene009435:CDS 55.0%
CCAACCATGTTGGTCCAGCA+AGG + chr3.4:3870921-3870940 None:intergenic 55.0%
CCTTGCTGGACCAACATGGT+TGG - chr3.4:3870918-3870937 MS.gene009435:CDS 55.0%
GACGTTGTGCATGAGCCATG+AGG + chr3.4:3870426-3870445 None:intergenic 55.0%
GACTCCTTGCTGGACCAACA+TGG - chr3.4:3870914-3870933 MS.gene009435:CDS 55.0%
GCAAACAGGGTGAAATGGCC+TGG - chr3.4:3870841-3870860 MS.gene009435:CDS 55.0%
GCTAACATGATCACCGCCCA+AGG - chr3.4:3870556-3870575 MS.gene009435:intron 55.0%
GGAATGGATCACCTCGACCT+TGG + chr3.4:3870576-3870595 None:intergenic 55.0%
GGGCGGTGATCATGTTAGCT+TGG + chr3.4:3870555-3870574 None:intergenic 55.0%
TCCTCACAAGGGTCAAGCTG+TGG - chr3.4:3870336-3870355 MS.gene009435:intron 55.0%
!! AACCAGTGCTCTGAGGGCAA+TGG - chr3.4:3869379-3869398 MS.gene009435:CDS 55.0%
!! CATGAGCCATGAGGGTGTCT+TGG + chr3.4:3870417-3870436 None:intergenic 55.0%
AAACCTTCCTGGGCAGGCCT+TGG - chr3.4:3870671-3870690 MS.gene009435:CDS 60.0%
ACCCCATGGAGACCAACCTG+GGG + chr3.4:3870752-3870771 None:intergenic 60.0%
ATGATCACCGCCCAAGGTCG+AGG - chr3.4:3870562-3870581 MS.gene009435:intron 60.0%
ATTGTTGCCAAGGCCTGCCC+AGG + chr3.4:3870681-3870700 None:intergenic 60.0%
CACCCCATGGAGACCAACCT+GGG + chr3.4:3870753-3870772 None:intergenic 60.0%
CTTTAGTGAGCCCCCCAGGT+TGG - chr3.4:3870737-3870756 MS.gene009435:CDS 60.0%
GACACTTTAGTGAGCCCCCC+AGG - chr3.4:3870733-3870752 MS.gene009435:CDS 60.0%
GCCACAGCTTGACCCTTGTG+AGG + chr3.4:3870340-3870359 None:intergenic 60.0%
TCACCCCATGGAGACCAACC+TGG + chr3.4:3870754-3870773 None:intergenic 60.0%
TGGATCACCTCGACCTTGGG+CGG + chr3.4:3870572-3870591 None:intergenic 60.0%
CCCATGGAGACCAACCTGGG+GGG + chr3.4:3870750-3870769 None:intergenic 65.0%
CCCCATGGAGACCAACCTGG+GGG + chr3.4:3870751-3870770 None:intergenic 65.0%
TTGCCAAGGCCTGCCCAGGA+AGG + chr3.4:3870677-3870696 None:intergenic 65.0%
!! CCCCCAGGTTGGTCTCCATG+GGG - chr3.4:3870748-3870767 MS.gene009435:CDS 65.0%
!! CCCCCCAGGTTGGTCTCCAT+GGG - chr3.4:3870747-3870766 MS.gene009435:CDS 65.0%
!! GCCCCCCAGGTTGGTCTCCA+TGG - chr3.4:3870746-3870765 MS.gene009435:CDS 70.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 3868925 3870975 3868925 ID=MS.gene009435
chr3.4 mRNA 3868925 3870975 3868925 ID=MS.gene009435.t1;Parent=MS.gene009435
chr3.4 exon 3870570 3870975 3870570 ID=MS.gene009435.t1.exon1;Parent=MS.gene009435.t1
chr3.4 CDS 3870570 3870975 3870570 ID=cds.MS.gene009435.t1;Parent=MS.gene009435.t1
chr3.4 exon 3869872 3870201 3869872 ID=MS.gene009435.t1.exon2;Parent=MS.gene009435.t1
chr3.4 CDS 3869872 3870201 3869872 ID=cds.MS.gene009435.t1;Parent=MS.gene009435.t1
chr3.4 exon 3868925 3869619 3868925 ID=MS.gene009435.t1.exon3;Parent=MS.gene009435.t1
chr3.4 CDS 3868925 3869619 3868925 ID=cds.MS.gene009435.t1;Parent=MS.gene009435.t1
Gene Sequence

>MS.gene009435

ATGAACAATCATTTCAAACTCAAACATAGAGTTGAATTCAGGGAAATGCTTGTTCAATTAGCCTTAAAACAAGCTCTTACCATGCAAAAAGAAGCACAAGAAAATTCTCAACACCAACAAAACTCATCAGTGCATAAAACGGTTCATGGTGATTGTTTGAAGCTATTTGAAAACACAATTTTTCATCTCAGCCGCACACTTGAAGGCTTAAACAATGCAAGCAAAAATTGTTCACCAAATGATGCACAAACATGGCTCACAACTTCTCTCACAAATATTGAAACATGTAAAAGTGGAGCTTTAGAACTCAATGCTCAAGATTTCAATTTCATTATGCAAACCAATGTCATTGAGATGATAAGGAACATCTTAGCCATCAATATGCATTTCATGAAACATGACAAAGGTACTAACAATGATTTTATATTTTTCTATTTAAAATTTGGGTCTTGCTAACCAGTGCTCTGAGGGCAATGGTTAATGCTAAGAATCCATTAGTAGAAATTATGTCTTGGAAACGTAAGTTTTTACATTTGTTTTAGCAATAATTACACAACTTTTTGATGCAAAGTTACTTATTTTAGTACTTTAACCAATGCCTCAAGGGCACTCATTAGCATTCCCCTTAAAATTTAAGTATTGTACTGCTAAAAAATAATTCAATAACTTTATTTAGGAGGAATTAAATGAGAGATAAATTATTGAATGTTTATTTGGTGATATATATATATATATATATAGTCATGCTGATCTAAATTTCTGAATCTGTTGCAGAAACAAAAGAAGAGTCATTTCCAAACTGGTTTTCTGTTCATGAAAGGAAGCTTTTGCAGTCCAAGTCTCCAGTAAAGTACAATCTTGTGGTTGCGAAAGATGGTTCTGGACAGTACAAGACTGTGCAAGCTGCTTTAAATGCTGCTGCTAAGAGAAAATACAGGACAAGGTTTGTGATACATGTTAAGAAAGGTGTTTATAGAGAGAACATTGAGGTTGCTGTTCATAATGATAACATTATGCTGGTTGGTGATGGAATGCAAAATACTATAATAACAAGCTCTAGAAGTGTTCAAGGTGGTTTTACCACTTATAGCTCTGCAACAGCAGGTGAGTTATATTCAATCTCCTTTCTCTTTTTATTTTCTTGTTATTAAAGAAATAAAATTAAATAGTGTCAAGTCAACTACTGAACATAGAAGATATAAAATAATTTCTTTGGTAAAATTTTATTTTATCTTTGAAGCTCTAAAATTTTATAATAAGATAGGTGTTTAATATTTATTATTATACTTTGCGGCTTTCTTACATACAATTACTTTCATTTTTTAAAATAATCACTCTTGGATCATTTAAATTTAGGTATTGATGGACTTCACTTCATTGCACGGGACATAACCTTCCAAAATACTGCTGGTCCTCACAAGGGTCAAGCTGTGGCGCTCCGATCAGCCTCTGATCTATCTGTCTTCTACCGGTGTGCTATATCAGGCTACCAAGACACCCTCATGGCTCATGCACAACGTCAATTTTACAGACAATGTTTCATTTATGGCACTGTAGACTTCATATTTGGCAATGCCGCAGTTGTATTCCAAAACTGTAACATTTTCGCAAGAAAGCCTTTGGATGGCCAAGCTAACATGATCACCGCCCAAGGTCGAGGTGATCCATTCCAAAACACTGGAATTTCATTCCACAATTGTCAAATTAGGGCTGCATCAGATCTAAAGCCTGTGGTTGACAAGTACAAAACCTTCCTGGGCAGGCCTTGGCAACAATATTCAAGAGTTATGGTGATGAAAACTTTTATGGACACTTTAGTGAGCCCCCCAGGTTGGTCTCCATGGGGTGACACTGATTTTGCTCAAGATACATTGTATTATGGGGAGTATGAGAATTATGGACCTGGTTCATCAACAGCAAACAGGGTGAAATGGCCTGGTTATCATGTGATAACAAACCCTAAGGAAGCATCAAAGTTTACGGTTACTGGACTCCTTGCTGGACCAACATGGTTGGCTACAACTACTGTGCCATTTACATCTGGTCTTTGA
Protein sequence

>MS.gene009435.t1

MNNHFKLKHRVEFREMLVQLALKQALTMQKEAQENSQHQQNSSVHKTVHGDCLKLFENTIFHLSRTLEGLNNASKNCSPNDAQTWLTTSLTNIETCKSGALELNAQDFNFIMQTNVIEMIRNILAINMHFMKHDKETKEESFPNWFSVHERKLLQSKSPVKYNLVVAKDGSGQYKTVQAALNAAAKRKYRTRFVIHVKKGVYRENIEVAVHNDNIMLVGDGMQNTIITSSRSVQGGFTTYSSATAGIDGLHFIARDITFQNTAGPHKGQAVALRSASDLSVFYRCAISGYQDTLMAHAQRQFYRQCFIYGTVDFIFGNAAVVFQNCNIFARKPLDGQANMITAQGRGDPFQNTGISFHNCQIRAASDLKPVVDKYKTFLGRPWQQYSRVMVMKTFMDTLVSPPGWSPWGDTDFAQDTLYYGEYENYGPGSSTANRVKWPGYHVITNPKEASKFTVTGLLAGPTWLATTTVPFTSGL