AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene059222

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene059222.t1 MTR_4g025690 97.137 489 14 0 1 489 25 513 0.0 993
MS.gene059222.t1 MTR_4g025730 74.338 491 117 5 1 489 26 509 0.0 754
MS.gene059222.t1 MTR_8g104655 50.584 514 220 10 5 489 33 541 0.0 518
MS.gene059222.t1 MTR_3g033690 53.414 498 217 9 4 489 32 526 7.61e-179 512
MS.gene059222.t1 MTR_4g035980 51.485 505 225 9 4 489 36 539 7.29e-178 510
MS.gene059222.t1 MTR_3g008640 53.307 499 217 10 4 489 32 527 1.41e-177 509
MS.gene059222.t1 MTR_3g008720 53.307 499 217 10 4 489 32 527 4.15e-177 508
MS.gene059222.t1 MTR_7g053360 53.106 499 218 10 4 489 32 527 7.96e-176 504
MS.gene059222.t1 MTR_3g008700 52.695 501 221 10 2 489 30 527 1.19e-175 504
MS.gene059222.t1 MTR_7g108905 49.209 506 227 11 8 485 49 552 1.93e-161 469
MS.gene059222.t1 MTR_8g104645 62.059 340 120 4 159 489 33 372 4.42e-155 446
MS.gene059222.t1 MTR_4g025670 53.052 426 181 8 72 484 65 484 7.74e-154 447
MS.gene059222.t1 MTR_8g104580 45.224 513 216 11 5 489 25 500 1.97e-152 444
MS.gene059222.t1 MTR_4g087980 51.242 443 191 6 66 489 80 516 2.54e-152 444
MS.gene059222.t1 MTR_8g023310 46.374 524 241 10 4 489 68 589 2.86e-149 439
MS.gene059222.t1 MTR_1g008140 43.916 526 247 10 8 489 86 607 3.92e-148 437
MS.gene059222.t1 MTR_2g044880 50.112 447 203 5 61 489 81 525 2.83e-147 432
MS.gene059222.t1 MTR_7g050980 47.656 512 230 13 6 489 54 555 3.68e-146 430
MS.gene059222.t1 MTR_3g091380 44.359 523 243 10 8 489 82 597 1.66e-145 430
MS.gene059222.t1 MTR_1g083520 48.622 508 216 13 8 485 32 524 4.17e-145 426
MS.gene059222.t1 MTR_7g050870 51.678 447 192 9 63 489 112 554 6.60e-145 427
MS.gene059222.t1 MTR_1g088020 47.753 534 217 17 8 489 32 555 3.31e-141 417
MS.gene059222.t1 MTR_7g112580 48.904 456 188 10 66 489 100 542 1.88e-138 410
MS.gene059222.t1 MTR_1g088225 47.566 534 216 18 8 489 32 553 4.42e-138 409
MS.gene059222.t1 MTR_1g054880 46.328 531 227 14 8 489 37 558 1.68e-137 408
MS.gene059222.t1 MTR_7g108890 46.139 531 229 15 8 489 40 562 1.96e-137 408
MS.gene059222.t1 MTR_7g108900 45.624 537 234 15 8 489 27 560 6.08e-136 404
MS.gene059222.t1 MTR_7g050950 48.998 449 200 10 63 489 112 553 8.84e-136 403
MS.gene059222.t1 MTR_4g087970 46.067 445 213 5 67 489 78 517 5.50e-133 395
MS.gene059222.t1 MTR_8g042910 45.772 544 224 16 8 489 78 612 1.19e-132 397
MS.gene059222.t1 MTR_7g108875 43.879 531 241 12 8 489 42 564 4.18e-132 394
MS.gene059222.t1 MTR_8g042900 43.883 515 251 13 4 489 76 581 7.92e-131 391
MS.gene059222.t1 MTR_3g010780 42.471 518 256 12 2 489 69 574 3.24e-127 382
MS.gene059222.t1 MTR_2g044810 42.200 500 267 9 1 485 38 530 6.46e-125 375
MS.gene059222.t1 MTR_4g124550 42.218 514 258 12 4 484 81 588 2.29e-123 373
MS.gene059222.t1 MTR_0458s0030 65.399 263 89 2 228 489 53 314 7.20e-123 362
MS.gene059222.t1 MTR_1g088040 65.399 263 89 2 228 489 53 314 7.20e-123 362
MS.gene059222.t1 MTR_3g010770 57.325 314 127 4 182 489 263 575 1.37e-121 367
MS.gene059222.t1 MTR_4g124520 41.352 503 255 13 1 485 37 517 2.06e-120 363
MS.gene059222.t1 MTR_8g023150 42.442 516 257 12 4 489 77 582 2.08e-120 365
MS.gene059222.t1 MTR_0618s0020 67.544 228 73 1 263 489 1 228 4.40e-116 341
MS.gene059222.t1 MTR_1g086190 39.836 487 253 11 37 485 75 559 1.16e-110 339
MS.gene059222.t1 MTR_1g086210 40.564 461 228 10 70 485 106 565 4.99e-110 338
MS.gene059222.t1 MTR_8g093460 38.803 518 268 14 2 483 41 545 1.77e-103 320
MS.gene059222.t1 MTR_3g095920 39.652 517 265 14 1 478 45 553 1.25e-102 318
MS.gene059222.t1 MTR_2g044660 48.632 329 161 5 159 484 304 627 1.52e-102 320
MS.gene059222.t1 MTR_1g083510 37.313 536 274 16 4 487 56 581 6.46e-101 315
MS.gene059222.t1 MTR_7g112590 36.278 532 280 14 4 486 59 580 1.33e-100 314
MS.gene059222.t1 MTR_7g110490 39.316 468 227 12 70 484 106 569 5.85e-100 312
MS.gene059222.t1 MTR_7g110510 39.316 468 227 12 70 484 106 569 5.85e-100 312
MS.gene059222.t1 MTR_7g110440 37.607 468 235 10 70 484 106 569 1.72e-99 310
MS.gene059222.t1 MTR_7g110560 37.452 518 277 13 4 485 39 545 4.34e-99 309
MS.gene059222.t1 MTR_1g083470 34.725 527 287 12 1 485 55 566 1.41e-98 308
MS.gene059222.t1 MTR_1g083500 34.725 527 287 12 1 485 55 566 1.41e-98 308
MS.gene059222.t1 MTR_1g083490 37.500 456 250 11 66 489 123 575 2.10e-96 303
MS.gene059222.t1 MTR_4g005170 38.274 452 244 12 66 485 123 571 2.68e-96 304
MS.gene059222.t1 MTR_4g005180 37.229 462 235 12 66 485 105 553 2.16e-94 297
MS.gene059222.t1 MTR_4g120290 36.782 522 278 15 4 485 59 568 1.74e-93 295
MS.gene059222.t1 MTR_2g436040 33.333 507 308 11 4 486 43 543 1.20e-92 294
MS.gene059222.t1 MTR_2g044810 53.846 247 112 2 240 485 216 461 2.35e-92 288
MS.gene059222.t1 MTR_4g120280 33.966 527 288 11 4 486 59 569 6.96e-92 291
MS.gene059222.t1 MTR_8g088610 43.976 332 177 7 161 486 45 373 3.27e-90 280
MS.gene059222.t1 MTR_2g436050 36.170 470 268 11 48 486 93 561 2.20e-87 279
MS.gene059222.t1 MTR_7g110420 36.268 477 243 12 70 489 106 578 2.40e-86 277
MS.gene059222.t1 MTR_5g083620 41.716 338 181 7 159 485 20 352 3.06e-85 267
MS.gene059222.t1 MTR_0458s0010 38.964 521 224 19 24 489 37 518 4.03e-85 271
MS.gene059222.t1 MTR_7g110430 33.191 467 223 10 70 484 106 535 5.27e-80 259
MS.gene059222.t1 MTR_2g105560 40.385 312 171 5 181 489 40 339 1.82e-73 236
MS.gene059222.t1 MTR_7g107180 40.317 315 175 5 178 489 3 307 2.09e-72 232
MS.gene059222.t1 MTR_6g024165 39.564 321 169 8 169 478 28 334 2.13e-66 217
MS.gene059222.t1 MTR_2g039530 42.434 304 158 6 158 456 28 319 6.73e-64 211
MS.gene059222.t1 MTR_7g111560 30.099 505 314 16 8 487 64 554 3.51e-62 212
MS.gene059222.t1 MTR_1g086540 36.646 322 178 6 169 478 37 344 6.44e-57 192
MS.gene059222.t1 MTR_8g102380 38.158 304 159 6 190 478 88 377 3.00e-55 189
MS.gene059222.t1 MTR_6g088810 39.145 304 154 7 190 478 90 377 1.36e-54 187
MS.gene059222.t1 MTR_6g012800 34.663 326 178 9 182 487 6 316 5.73e-53 181
MS.gene059222.t1 MTR_6g042660 35.764 288 157 7 171 451 30 296 9.98e-49 170
MS.gene059222.t1 MTR_7g011530 33.230 322 184 8 184 487 8 316 4.04e-47 166
MS.gene059222.t1 MTR_8g102380 40.726 248 121 6 190 422 88 324 4.28e-47 166
MS.gene059222.t1 MTR_7g092780 32.132 333 195 7 159 478 73 387 5.33e-47 167
MS.gene059222.t1 MTR_5g083590 32.143 308 155 5 182 485 7 264 7.17e-46 161
MS.gene059222.t1 MTR_5g095490 33.876 307 171 6 188 478 67 357 9.16e-45 160
MS.gene059222.t1 MTR_6g042690 34.507 284 153 8 182 451 39 303 1.43e-43 157
MS.gene059222.t1 MTR_4g124740 34.091 308 170 7 182 478 54 339 2.20e-43 157
MS.gene059222.t1 MTR_2g033970 32.390 318 191 6 172 480 46 348 7.28e-43 155
MS.gene059222.t1 MTR_1g033050 32.982 285 168 5 177 451 13 284 9.71e-43 154
MS.gene059222.t1 MTR_7g102600 32.459 305 184 6 184 478 101 393 1.11e-42 156
MS.gene059222.t1 MTR_5g012600 31.529 314 187 7 182 481 63 362 4.53e-40 148
MS.gene059222.t1 MTR_5g088300 32.215 298 183 5 182 478 37 316 6.39e-39 144
MS.gene059222.t1 MTR_5g093970 33.115 305 178 9 182 478 43 329 7.82e-39 144
MS.gene059222.t1 MTR_2g436600 31.348 319 184 9 172 480 45 338 1.96e-37 140
MS.gene059222.t1 MTR_2g073390 30.247 324 203 8 169 483 58 367 4.20e-37 140
MS.gene059222.t1 MTR_4g074290 31.193 327 196 9 169 483 58 367 9.37e-36 136
MS.gene059222.t1 MTR_4g051810 32.441 299 182 6 182 478 37 317 1.03e-35 135
MS.gene059222.t1 MTR_5g088280 31.229 301 188 5 179 478 36 318 2.42e-35 134
MS.gene059222.t1 MTR_5g088290 31.229 301 183 7 182 478 48 328 2.35e-34 132
MS.gene059222.t1 MTR_0009s0350 28.834 326 205 8 169 483 58 367 3.94e-32 126
MS.gene059222.t1 MTR_0009s0340 28.834 326 205 8 169 483 58 367 3.94e-32 126
MS.gene059222.t1 MTR_8g036225 27.302 315 200 6 182 486 33 328 4.61e-30 120
MS.gene059222.t1 MTR_6g025590 37.569 181 103 3 169 339 36 216 1.19e-28 114
MS.gene059222.t1 MTR_2g436070 27.957 279 157 10 4 251 56 321 1.81e-19 90.5
MS.gene059222.t1 MTR_4g130790 28.112 249 147 8 185 420 25 254 5.93e-16 78.2
MS.gene059222.t1 MTR_0408s0030 40.404 99 46 3 355 451 36 123 1.04e-15 74.7
MS.gene059222.t1 MTR_6g024200 34.375 160 84 5 320 478 137 276 2.46e-15 76.6
MS.gene059222.t1 MTR_7g102580 40.659 91 44 2 249 334 68 153 2.72e-12 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene059222.t1 AT2G45220 67.213 488 154 4 3 489 29 511 0.0 699
MS.gene059222.t1 AT5G51490 50.992 504 222 9 7 489 37 536 3.09e-176 506
MS.gene059222.t1 AT3G47400 49.609 512 233 9 3 489 83 594 1.26e-172 499
MS.gene059222.t1 AT5G51500 50.598 502 227 9 7 489 41 540 4.70e-172 495
MS.gene059222.t1 AT4G00190 53.388 487 202 9 8 489 8 474 7.73e-172 492
MS.gene059222.t1 AT1G23200 47.925 530 215 13 8 489 38 554 2.01e-153 448
MS.gene059222.t1 AT3G49220 45.383 509 248 8 8 489 91 596 2.52e-147 434
MS.gene059222.t1 AT3G43270 48.857 481 214 8 39 489 47 525 1.15e-146 430
MS.gene059222.t1 AT3G49220 45.383 509 248 8 8 489 136 641 1.96e-146 434
MS.gene059222.t1 AT5G53370 44.227 511 252 8 8 489 79 585 4.00e-145 428
MS.gene059222.t1 AT3G60730 47.629 464 201 7 62 487 58 517 7.22e-145 425
MS.gene059222.t1 AT5G53370 44.227 511 252 8 8 489 115 621 1.11e-144 428
MS.gene059222.t1 AT3G14310 48.718 468 191 8 68 486 122 589 1.89e-144 427
MS.gene059222.t1 AT3G59010 49.471 473 207 8 39 489 66 528 4.26e-144 424
MS.gene059222.t1 AT1G53830 45.472 530 241 11 4 489 62 587 1.54e-141 419
MS.gene059222.t1 AT4G02320 47.473 455 212 10 60 489 66 518 6.03e-141 415
MS.gene059222.t1 AT1G11580 49.658 439 197 7 68 485 118 553 3.92e-138 409
MS.gene059222.t1 AT1G11580 49.658 439 197 7 68 485 139 574 1.06e-137 409
MS.gene059222.t1 AT4G33220 43.725 510 243 8 21 489 17 523 2.59e-137 406
MS.gene059222.t1 AT4G33220 56.627 332 141 2 161 489 71 402 1.16e-134 395
MS.gene059222.t1 AT3G05620 49.011 455 188 11 67 489 99 541 3.04e-134 399
MS.gene059222.t1 AT2G43050 48.843 432 189 10 75 489 100 516 1.13e-129 386
MS.gene059222.t1 AT4G02330 44.404 545 232 17 8 489 35 571 2.71e-129 387
MS.gene059222.t1 AT4G02300 45.393 445 211 10 69 486 90 529 1.24e-127 382
MS.gene059222.t1 AT1G02810 42.523 555 237 14 8 489 32 577 2.72e-126 380
MS.gene059222.t1 AT2G26450 43.404 470 238 11 38 484 142 606 4.06e-124 375
MS.gene059222.t1 AT5G04960 41.683 511 255 13 4 487 68 562 5.59e-123 371
MS.gene059222.t1 AT3G10720 42.251 542 244 16 8 489 83 615 6.43e-122 370
MS.gene059222.t1 AT2G47550 46.220 463 201 11 72 489 99 558 9.76e-122 367
MS.gene059222.t1 AT5G20860 40.856 514 270 12 2 489 33 538 2.75e-121 365
MS.gene059222.t1 AT3G10710 42.495 513 247 13 4 489 70 561 7.25e-121 365
MS.gene059222.t1 AT1G53840 40.385 520 259 12 4 483 73 581 6.75e-120 363
MS.gene059222.t1 AT2G26440 40.116 516 260 12 7 485 40 543 2.06e-118 358
MS.gene059222.t1 AT3G05610 41.501 453 233 10 66 489 116 565 3.82e-118 362
MS.gene059222.t1 AT5G04970 41.697 542 247 17 8 489 88 620 5.02e-117 357
MS.gene059222.t1 AT3G06830 39.630 487 254 11 39 489 86 568 8.97e-117 355
MS.gene059222.t1 AT5G49180 40.232 517 269 15 4 485 55 566 9.57e-117 355
MS.gene059222.t1 AT5G27870 41.348 445 235 8 66 485 115 558 1.15e-114 355
MS.gene059222.t1 AT4G33230 42.576 458 226 11 60 484 148 601 1.19e-112 345
MS.gene059222.t1 AT3G14300 39.764 508 259 12 8 479 463 959 9.04e-111 350
MS.gene059222.t1 AT3G10720 60.385 260 101 2 231 489 1 259 9.28e-111 329
MS.gene059222.t1 AT4G15980 47.278 349 175 5 147 487 353 700 8.30e-106 331
MS.gene059222.t1 AT4G15980 47.278 349 175 5 147 487 363 710 1.51e-105 330
MS.gene059222.t1 AT3G27980 42.893 394 216 5 103 489 106 497 1.06e-104 322
MS.gene059222.t1 AT1G11590 43.000 400 215 5 101 489 127 524 2.59e-101 314
MS.gene059222.t1 AT4G03930 42.462 398 216 6 103 489 129 524 5.67e-101 313
MS.gene059222.t1 AT5G64640 39.265 517 269 17 3 481 92 601 1.03e-98 309
MS.gene059222.t1 AT5G09760 38.835 515 263 17 3 477 44 546 1.48e-98 307
MS.gene059222.t1 AT5G09760 38.641 515 264 16 3 477 70 572 4.17e-98 307
MS.gene059222.t1 AT5G20860 37.728 493 245 12 2 468 33 489 1.02e-97 304
MS.gene059222.t1 AT2G47030 44.540 348 173 6 160 489 243 588 2.74e-85 274
MS.gene059222.t1 AT2G47040 43.768 345 177 6 160 489 253 595 8.95e-82 265
MS.gene059222.t1 AT3G62170 41.737 357 180 6 154 484 229 583 1.16e-81 265
MS.gene059222.t1 AT1G11370 49.398 249 123 2 174 419 38 286 4.42e-81 253
MS.gene059222.t1 AT5G19730 37.864 309 165 6 183 478 87 381 1.66e-54 187
MS.gene059222.t1 AT3G17060 33.229 319 185 7 172 479 36 337 4.72e-49 172
MS.gene059222.t1 AT1G05310 33.934 333 189 8 159 478 74 388 3.28e-48 171
MS.gene059222.t1 AT3G29090 35.106 282 161 4 182 451 7 278 4.72e-48 168
MS.gene059222.t1 AT2G36700 33.444 302 182 4 184 478 44 333 3.38e-47 167
MS.gene059222.t1 AT2G21610 32.890 301 179 7 182 478 51 332 1.63e-45 162
MS.gene059222.t1 AT2G21610 32.890 301 179 7 182 478 56 337 2.25e-45 162
MS.gene059222.t1 AT5G07430 31.081 370 229 8 119 478 2 355 2.40e-45 162
MS.gene059222.t1 AT2G36710 32.372 312 189 7 182 483 91 390 2.65e-44 161
MS.gene059222.t1 AT5G55590 33.739 329 190 8 164 478 64 378 2.74e-43 157
MS.gene059222.t1 AT5G07420 32.075 318 192 7 169 478 54 355 2.11e-42 154
MS.gene059222.t1 AT5G18990 33.441 311 177 7 182 478 29 323 4.79e-42 152
MS.gene059222.t1 AT5G47500 30.699 329 191 7 164 478 52 357 6.54e-42 153
MS.gene059222.t1 AT2G19150 33.754 317 179 9 182 485 41 339 1.67e-41 151
MS.gene059222.t1 AT3G24130 31.348 319 185 8 182 484 34 334 1.15e-40 149
MS.gene059222.t1 AT5G55590 35.165 273 152 7 164 422 64 325 2.89e-40 148
MS.gene059222.t1 AT1G44980 41.489 188 104 2 162 343 54 241 1.04e-39 144
MS.gene059222.t1 AT5G61680 31.329 316 191 8 173 478 59 358 4.15e-39 145
MS.gene059222.t1 AT1G69940 29.141 326 206 7 162 478 46 355 1.10e-37 142
MS.gene059222.t1 AT5G07410 29.141 326 206 7 162 478 46 355 7.91e-37 139
MS.gene059222.t1 AT5G26810 32.372 312 184 8 182 478 34 333 2.85e-36 137
MS.gene059222.t1 AT3G49220 36.697 218 111 6 68 259 194 410 6.63e-35 135
MS.gene059222.t1 AT2G47280 32.476 311 178 8 182 478 35 327 1.01e-34 133
MS.gene059222.t1 AT2G19150 34.524 252 141 7 182 422 41 279 3.08e-34 130

Find 102 sgRNAs with CRISPR-Local

Find 143 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGCAAATATGTTACAATTT+TGG 0.106193 4.1:+73200258 None:intergenic
GGTACGGTTGATTTCATATT+TGG 0.122126 4.1:-73200299 MS.gene059222:CDS
TACAAACAATGTAACATTTA+TGG 0.134217 4.1:-73200320 MS.gene059222:CDS
GATAAGTCTTAACAGAATTT+TGG 0.160141 4.1:+73200117 None:intergenic
GCATTTCTATTCACAATTCT+AGG 0.200545 4.1:-73200169 MS.gene059222:CDS
TACCTACCTTTGTTATTCTT+TGG 0.232922 4.1:+73201484 None:intergenic
GCATTAAGTACTTTATATTA+TGG 0.242821 4.1:-73199993 MS.gene059222:CDS
CCAAAGTTTAACTTCATTCA+TGG 0.264428 4.1:+73202092 None:intergenic
TGTTGTTGGAGATGGATTTA+TGG 0.279809 4.1:-73200489 MS.gene059222:CDS
TGGCTCAAGATATCACATTT+AGG 0.292215 4.1:-73200469 MS.gene059222:CDS
ACTGTATCAAATTTCATTGC+TGG 0.309927 4.1:-73199873 MS.gene059222:CDS
TTCACGTGAGTTACTACATT+TGG 0.320043 4.1:+73201906 None:intergenic
TACACACAAGGTTGAGGATT+TGG 0.321560 4.1:+73202057 None:intergenic
GTGATTGTGTTGACTTTGTT+TGG 0.331970 4.1:+73200227 None:intergenic
TGATTTCTTCAAACTTTCAT+TGG 0.341515 4.1:-73201983 MS.gene059222:CDS
GTAAATGGCACTTTGGTTGC+AGG 0.349440 4.1:+73199837 None:intergenic
TTCTAGGGTTACGGCTGCTT+CGG 0.355427 4.1:-73200153 MS.gene059222:CDS
AAGGTTGAGGATTTGGTGTT+TGG 0.356796 4.1:+73202064 None:intergenic
AATTCTGTTAAGACTTATCT+TGG 0.359832 4.1:-73200113 MS.gene059222:CDS
ATCCAGCTGGTTGGTTAGAA+TGG 0.361304 4.1:-73200028 MS.gene059222:CDS
AATTAAAGAGTAGATGTAAA+TGG 0.364262 4.1:+73199822 None:intergenic
CAAGCTGTTGCATTGCGTTC+CGG 0.373938 4.1:-73200422 MS.gene059222:CDS
GACCATGGAAGCAATATTCT+AGG 0.374299 4.1:-73200088 MS.gene059222:CDS
CATGCAAACACTCTTTCATT+AGG 0.385373 4.1:-73201931 MS.gene059222:CDS
CAATGAACAAGTTGAGATAA+AGG 0.386142 4.1:-73201434 MS.gene059222:CDS
TATTCACAATTCTAGGGTTA+CGG 0.397130 4.1:-73200162 MS.gene059222:CDS
TAGCAATTTAGTAACATTGT+TGG 0.401894 4.1:+73201690 None:intergenic
GGGAACCCATCTTTGTAGCT+AGG 0.418473 4.1:+73201628 None:intergenic
GAATAGAAATGCCAGTGTTT+TGG 0.434967 4.1:+73200180 None:intergenic
GCAAAGAATATTATGTTGGT+TGG 0.435785 4.1:-73201412 MS.gene059222:CDS
ATTCTTTGGTATAGCATTCA+CGG 0.447221 4.1:+73201498 None:intergenic
CCATGAATGAAGTTAAACTT+TGG 0.447619 4.1:-73202092 MS.gene059222:CDS
AACGCAATGCAACAGCTTGA+TGG 0.450785 4.1:+73200426 None:intergenic
CATTTCTATTCACAATTCTA+GGG 0.450955 4.1:-73200168 MS.gene059222:CDS
TTTGCAGCTGTTGTTGGAGA+TGG 0.451928 4.1:-73200497 MS.gene059222:intron
GGCTGCTTCGGATTTGAAAC+CGG 0.452219 4.1:-73200141 MS.gene059222:CDS
TAGTAGCAAAAGACGGGTCC+GGG 0.473703 4.1:-73201543 MS.gene059222:CDS
CACCTGGCCTAACCCATGTT+GGG 0.479286 4.1:+73201608 None:intergenic
AATATTATGTTGGTTGGAGA+TGG 0.480645 4.1:-73201406 MS.gene059222:CDS
ATGAGTTTGTGTTGGTAAGA+AGG 0.482647 4.1:+73202018 None:intergenic
AAAGGCAAAGAATATTATGT+TGG 0.483951 4.1:-73201416 MS.gene059222:CDS
CCATTTGACACGGTTTCTTG+TGG 0.485738 4.1:+73199941 None:intergenic
GAGTATATGAACACTGGTCT+TGG 0.488203 4.1:-73199969 MS.gene059222:CDS
TTGTGTTGACTTTGTTTGGA+GGG 0.490707 4.1:+73200231 None:intergenic
CAAGAACCTAGCTACAAAGA+TGG 0.493394 4.1:-73201634 MS.gene059222:CDS
ATTTCATTGCTGGAAATGCT+TGG 0.496886 4.1:-73199863 MS.gene059222:CDS
TGTAGTAGCTTTCTATCACC+TGG 0.499249 4.1:+73201592 None:intergenic
CCACAAGAAACCGTGTCAAA+TGG 0.501895 4.1:-73199941 MS.gene059222:CDS
GCCCTAGAATATTGCTTCCA+TGG 0.507314 4.1:+73200086 None:intergenic
TAAATTGCTAAGTAACACTT+TGG 0.507939 4.1:-73201677 MS.gene059222:CDS
AGCTTGATGGTTCTTTGCAC+CGG 0.510568 4.1:+73200439 None:intergenic
GATAGTTTGATTCATCCAGC+TGG 0.516549 4.1:-73200041 MS.gene059222:CDS
TCACCTGGCCTAACCCATGT+TGG 0.518597 4.1:+73201607 None:intergenic
TTTGTTTGATGAGTTTGTGT+TGG 0.521126 4.1:+73202010 None:intergenic
AGTAGATGTAAATGGCACTT+TGG 0.524094 4.1:+73199830 None:intergenic
ACCGGTAGCAAAAGCGTTGG+AGG 0.524747 4.1:-73201364 MS.gene059222:CDS
AAATACAGACAAGTCTGATC+CGG 0.525381 4.1:+73200403 None:intergenic
ATGTTGGTTGGAGATGGTAT+TGG 0.525732 4.1:-73201400 MS.gene059222:CDS
AAAGCTGGATTCTATGATCT+TGG 0.527056 4.1:-73201742 MS.gene059222:CDS
GTAGTAGCAAAAGACGGGTC+CGG 0.527702 4.1:-73201544 MS.gene059222:CDS
TTCCCAACATGGGTTAGGCC+AGG 0.531406 4.1:-73201610 MS.gene059222:CDS
ACAATGTAACATTTATGGTA+CGG 0.531513 4.1:-73200315 MS.gene059222:CDS
AAGAAATACACACAAGGTTG+AGG 0.533464 4.1:+73202051 None:intergenic
ATGGGTTCCCAACATGGGTT+AGG 0.533950 4.1:-73201615 MS.gene059222:CDS
TGAGTTTGTGTTGGTAAGAA+GGG 0.534506 4.1:+73202019 None:intergenic
ACAAAGATGGGTTCCCAACA+TGG 0.535204 4.1:-73201621 MS.gene059222:CDS
AGCTTTACATGTCTCAAGGT+TGG 0.546562 4.1:+73201759 None:intergenic
CTCCATTCTAACCAACCAGC+TGG 0.548616 4.1:+73200026 None:intergenic
AACCTTGAGACATGTAAAGC+TGG 0.551306 4.1:-73201757 MS.gene059222:CDS
AAGAACCTAGCTACAAAGAT+GGG 0.557927 4.1:-73201633 MS.gene059222:CDS
TAGGTATGTTATACATGTGA+AGG 0.561706 4.1:-73201467 MS.gene059222:CDS
CTCAAGTTGATGCTCAAACA+TGG 0.563888 4.1:-73201798 MS.gene059222:CDS
ATCCAGCTTTACATGTCTCA+AGG 0.564983 4.1:+73201755 None:intergenic
ATTGTGTTGACTTTGTTTGG+AGG 0.571190 4.1:+73200230 None:intergenic
ACATTCCGTTCTGCCACTGT+TGG 0.587276 4.1:-73201331 MS.gene059222:intron
GATAACCTTGCCATTTGACA+CGG 0.589011 4.1:+73199931 None:intergenic
ACGTAGTAGTAGCAAAAGAC+GGG 0.589841 4.1:-73201549 MS.gene059222:CDS
AACGTAGTAGTAGCAAAAGA+CGG 0.590566 4.1:-73201550 MS.gene059222:CDS
GTTTGATTCATCCAGCTGGT+TGG 0.591836 4.1:-73200037 MS.gene059222:CDS
ACCATGGAAGCAATATTCTA+GGG 0.592357 4.1:-73200087 MS.gene059222:CDS
ATTGGTAAAACTGTAATCAC+CGG 0.592601 4.1:-73201382 MS.gene059222:CDS
CTAATGAAAGAGTGTTTGCA+TGG 0.596121 4.1:+73201932 None:intergenic
AGACTTATCTTGGAAGACCA+TGG 0.599466 4.1:-73200103 MS.gene059222:CDS
ATCACCGGTAGCAAAAGCGT+TGG 0.601310 4.1:-73201367 MS.gene059222:CDS
AGAAACCGTGTCAAATGGCA+AGG 0.607462 4.1:-73199936 MS.gene059222:CDS
TACCAAAGAATAACAAAGGT+AGG 0.608433 4.1:-73201486 MS.gene059222:CDS
GGTAGCAAAAGCGTTGGAGG+AGG 0.610712 4.1:-73201361 MS.gene059222:CDS
ATCACATTTAGGAACACTGC+CGG 0.612058 4.1:-73200458 MS.gene059222:CDS
GTAACATTGTTGGACATGAG+AGG 0.612493 4.1:+73201700 None:intergenic
CAAAGATGGGTTCCCAACAT+GGG 0.625227 4.1:-73201620 MS.gene059222:CDS
ACAGATCCTAACCAAAACAC+TGG 0.626471 4.1:-73200191 MS.gene059222:CDS
ACTAACAGTTGTGTACGTCC+CGG 0.629155 4.1:+73201525 None:intergenic
GCTATACCAAAGAATAACAA+AGG 0.633072 4.1:-73201490 MS.gene059222:CDS
TATGGAGAGTATATGAACAC+TGG 0.633586 4.1:-73199975 MS.gene059222:CDS
TATGTTATACATGTGAAGGC+TGG 0.634350 4.1:-73201463 MS.gene059222:CDS
ACTTACCAACAGTGGCAGAA+CGG 0.640460 4.1:+73201326 None:intergenic
GTTCAAGAAAGAGCACTCAA+AGG 0.644742 4.1:-73201955 MS.gene059222:CDS
GTCAACACAATCACTGCACA+AGG 0.651414 4.1:-73200218 MS.gene059222:CDS
CTGCTCAAGAAATACACACA+AGG 0.658259 4.1:+73202045 None:intergenic
GCTGGTTGGTTAGAATGGAG+TGG 0.670039 4.1:-73200023 MS.gene059222:CDS
CAGTAACTACTTACCAACAG+TGG 0.681222 4.1:+73201318 None:intergenic
AGTGGCAGAACGGAATGTTG+TGG 0.699012 4.1:+73201336 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTAACATTATTTTTGTTT+TGG + chr4.1:73200838-73200857 None:intergenic 10.0%
!! CAATTTATTTAAATTGTTGA+AGG - chr4.1:73201216-73201235 MS.gene059222:intron 15.0%
!! TATTAATGTTAAAAACTAAG+AGG + chr4.1:73201353-73201372 None:intergenic 15.0%
!!! CTTGCAATTTTTTTTTATAT+AGG + chr4.1:73201052-73201071 None:intergenic 15.0%
!! GCATTAAGTACTTTATATTA+TGG - chr4.1:73201921-73201940 MS.gene059222:CDS 20.0%
!! TACAAACAATGTAACATTTA+TGG - chr4.1:73201594-73201613 MS.gene059222:CDS 20.0%
!!! CTTTTATGTATTTATAGAAC+CGG - chr4.1:73200999-73201018 MS.gene059222:intron 20.0%
!!! TCTTGCAATTTTTTTGATAT+AGG + chr4.1:73201091-73201110 None:intergenic 20.0%
!!! TGAAATGTTGCTTATTATTA+AGG - chr4.1:73200659-73200678 MS.gene059222:intron 20.0%
!!! TTGCAAATATGTTACAATTT+TGG + chr4.1:73201659-73201678 None:intergenic 20.0%
!!! TTTGTTCCATTTTTTTAATG+CGG - chr4.1:73201160-73201179 MS.gene059222:intron 20.0%
!!! TTTTATAGATGTAGTTTTGA+AGG - chr4.1:73201531-73201550 MS.gene059222:CDS 20.0%
! AAAGGCAAAGAATATTATGT+TGG - chr4.1:73200498-73200517 MS.gene059222:intron 25.0%
! AATTCTGTTAAGACTTATCT+TGG - chr4.1:73201801-73201820 MS.gene059222:CDS 25.0%
! ACAATGTAACATTTATGGTA+CGG - chr4.1:73201599-73201618 MS.gene059222:CDS 25.0%
! ATTATTAAGGTTGCCAAAAT+CGG - chr4.1:73200672-73200691 MS.gene059222:intron 25.0%
! CATTTCTATTCACAATTCTA+GGG - chr4.1:73201746-73201765 MS.gene059222:CDS 25.0%
! GTAAAACCGCATTAAAAAAA+TGG + chr4.1:73201169-73201188 None:intergenic 25.0%
! TAAATTGCTAAGTAACACTT+TGG - chr4.1:73200237-73200256 MS.gene059222:CDS 25.0%
! TAGCAATTTAGTAACATTGT+TGG + chr4.1:73200227-73200246 None:intergenic 25.0%
! TGATTTCTTCAAACTTTCAT+TGG - chr4.1:73199931-73199950 MS.gene059222:CDS 25.0%
!! GATAAGTCTTAACAGAATTT+TGG + chr4.1:73201800-73201819 None:intergenic 25.0%
!!! AACACTTTGTTTTAGTATCT+AGG + chr4.1:73200096-73200115 None:intergenic 25.0%
!!! ACACTTTGTTTTAGTATCTA+GGG + chr4.1:73200095-73200114 None:intergenic 25.0%
!!! CTGAATTTTTGGATTTAAGT+CGG - chr4.1:73201258-73201277 MS.gene059222:intron 25.0%
!!! TGAATTTTTGGATTTAAGTC+GGG - chr4.1:73201259-73201278 MS.gene059222:intron 25.0%
!!! TTTTTTAATGCGGTTTTACT+AGG - chr4.1:73201170-73201189 MS.gene059222:intron 25.0%
AAATTGCAGAATTCCAAAAC+AGG + chr4.1:73201202-73201221 None:intergenic 30.0%
ACTGTATCAAATTTCATTGC+TGG - chr4.1:73202041-73202060 MS.gene059222:CDS 30.0%
ATTCTTTGGTATAGCATTCA+CGG + chr4.1:73200419-73200438 None:intergenic 30.0%
ATTGGTAAAACTGTAATCAC+CGG - chr4.1:73200532-73200551 MS.gene059222:intron 30.0%
CGACTTAAATCCAAAAATTC+AGG + chr4.1:73201260-73201279 None:intergenic 30.0%
GCAAAGAATATTATGTTGGT+TGG - chr4.1:73200502-73200521 MS.gene059222:intron 30.0%
GCATTTCTATTCACAATTCT+AGG - chr4.1:73201745-73201764 MS.gene059222:CDS 30.0%
GCTATACCAAAGAATAACAA+AGG - chr4.1:73200424-73200443 MS.gene059222:CDS 30.0%
TACCAAAGAATAACAAAGGT+AGG - chr4.1:73200428-73200447 MS.gene059222:CDS 30.0%
TAGGTATGTTATACATGTGA+AGG - chr4.1:73200447-73200466 MS.gene059222:CDS 30.0%
TTATTAAGGTTGCCAAAATC+GGG - chr4.1:73200673-73200692 MS.gene059222:intron 30.0%
TTTGTTTGATGAGTTTGTGT+TGG + chr4.1:73199907-73199926 None:intergenic 30.0%
! AATATTATGTTGGTTGGAGA+TGG - chr4.1:73200508-73200527 MS.gene059222:intron 30.0%
! CAATGAACAAGTTGAGATAA+AGG - chr4.1:73200480-73200499 MS.gene059222:CDS 30.0%
! GTTGCAAAATCCTGAATTTT+TGG - chr4.1:73201247-73201266 MS.gene059222:intron 30.0%
! TAAAATCCTGTGATTTTGCA+TGG - chr4.1:73201127-73201146 MS.gene059222:intron 30.0%
!! TACCTACCTTTGTTATTCTT+TGG + chr4.1:73200433-73200452 None:intergenic 30.0%
!! TATTCACAATTCTAGGGTTA+CGG - chr4.1:73201752-73201771 MS.gene059222:CDS 30.0%
AAAGCTGGATTCTATGATCT+TGG - chr4.1:73200172-73200191 MS.gene059222:CDS 35.0%
AAATACAGACAAGTCTGATC+CGG + chr4.1:73201514-73201533 None:intergenic 35.0%
AACGTAGTAGTAGCAAAAGA+CGG - chr4.1:73200364-73200383 MS.gene059222:CDS 35.0%
AAGAAATACACACAAGGTTG+AGG + chr4.1:73199866-73199885 None:intergenic 35.0%
AAGAACCTAGCTACAAAGAT+GGG - chr4.1:73200281-73200300 MS.gene059222:CDS 35.0%
ACCATGGAAGCAATATTCTA+GGG - chr4.1:73201827-73201846 MS.gene059222:CDS 35.0%
AGTAGATGTAAATGGCACTT+TGG + chr4.1:73202087-73202106 None:intergenic 35.0%
ATTTCATTGCTGGAAATGCT+TGG - chr4.1:73202051-73202070 MS.gene059222:CDS 35.0%
CATGCAAACACTCTTTCATT+AGG - chr4.1:73199983-73200002 MS.gene059222:CDS 35.0%
GTATTTATAGAACCGGATCA+CGG - chr4.1:73201006-73201025 MS.gene059222:intron 35.0%
TAAAAGACCAAGTTCTGTCT+AGG + chr4.1:73200985-73201004 None:intergenic 35.0%
TAAACAACCTAGACAGAACT+TGG - chr4.1:73200975-73200994 MS.gene059222:intron 35.0%
TATGTTATACATGTGAAGGC+TGG - chr4.1:73200451-73200470 MS.gene059222:CDS 35.0%
TATTTATAGAACCGGATCAC+GGG - chr4.1:73201007-73201026 MS.gene059222:intron 35.0%
TGAAATCCATGCAAAATCAC+AGG + chr4.1:73201136-73201155 None:intergenic 35.0%
TGGCTCAAGATATCACATTT+AGG - chr4.1:73201445-73201464 MS.gene059222:CDS 35.0%
TTCACGTGAGTTACTACATT+TGG + chr4.1:73200011-73200030 None:intergenic 35.0%
TTTATATAGGTCAATCGACC+CGG + chr4.1:73201039-73201058 None:intergenic 35.0%
! ATGAGTTTGTGTTGGTAAGA+AGG + chr4.1:73199899-73199918 None:intergenic 35.0%
! GAATAGAAATGCCAGTGTTT+TGG + chr4.1:73201737-73201756 None:intergenic 35.0%
! GGTACGGTTGATTTCATATT+TGG - chr4.1:73201615-73201634 MS.gene059222:CDS 35.0%
! GTCTTAACAGAATTTTGGAC+CGG + chr4.1:73201795-73201814 None:intergenic 35.0%
! TATGGAGAGTATATGAACAC+TGG - chr4.1:73201939-73201958 MS.gene059222:CDS 35.0%
! TCTTTTGCTACTACTACGTT+AGG + chr4.1:73200364-73200383 None:intergenic 35.0%
! TGAGTTTGTGTTGGTAAGAA+GGG + chr4.1:73199898-73199917 None:intergenic 35.0%
! TGTTGTTGGAGATGGATTTA+TGG - chr4.1:73201425-73201444 MS.gene059222:CDS 35.0%
!! ATTGTGTTGACTTTGTTTGG+AGG + chr4.1:73201687-73201706 None:intergenic 35.0%
!! CTAATGAAAGAGTGTTTGCA+TGG + chr4.1:73199985-73200004 None:intergenic 35.0%
!! GTGATTGTGTTGACTTTGTT+TGG + chr4.1:73201690-73201709 None:intergenic 35.0%
!! TTGTGTTGACTTTGTTTGGA+GGG + chr4.1:73201686-73201705 None:intergenic 35.0%
AACCTTGAGACATGTAAAGC+TGG - chr4.1:73200157-73200176 MS.gene059222:CDS 40.0%
ACAGATCCTAACCAAAACAC+TGG - chr4.1:73201723-73201742 MS.gene059222:CDS 40.0%
ACGTAGTAGTAGCAAAAGAC+GGG - chr4.1:73200365-73200384 MS.gene059222:CDS 40.0%
ACTCACGTGAAAAATCAGCT+TGG - chr4.1:73200020-73200039 MS.gene059222:CDS 40.0%
AGACTTATCTTGGAAGACCA+TGG - chr4.1:73201811-73201830 MS.gene059222:CDS 40.0%
AGCTTTACATGTCTCAAGGT+TGG + chr4.1:73200158-73200177 None:intergenic 40.0%
ATCACATTTAGGAACACTGC+CGG - chr4.1:73201456-73201475 MS.gene059222:CDS 40.0%
ATCCAGCTTTACATGTCTCA+AGG + chr4.1:73200162-73200181 None:intergenic 40.0%
CAAGAACCTAGCTACAAAGA+TGG - chr4.1:73200280-73200299 MS.gene059222:CDS 40.0%
CAGTAACTACTTACCAACAG+TGG + chr4.1:73200599-73200618 None:intergenic 40.0%
CTCACGTGAAAAATCAGCTT+GGG - chr4.1:73200021-73200040 MS.gene059222:CDS 40.0%
CTGCTCAAGAAATACACACA+AGG + chr4.1:73199872-73199891 None:intergenic 40.0%
GACCATGGAAGCAATATTCT+AGG - chr4.1:73201826-73201845 MS.gene059222:CDS 40.0%
GATAGTTTGATTCATCCAGC+TGG - chr4.1:73201873-73201892 MS.gene059222:CDS 40.0%
GTAACATTGTTGGACATGAG+AGG + chr4.1:73200217-73200236 None:intergenic 40.0%
TACACACAAGGTTGAGGATT+TGG + chr4.1:73199860-73199879 None:intergenic 40.0%
TGTAGTAGCTTTCTATCACC+TGG + chr4.1:73200325-73200344 None:intergenic 40.0%
! ATGTTGGTTGGAGATGGTAT+TGG - chr4.1:73200514-73200533 MS.gene059222:intron 40.0%
! CAAAATCGGGAATTAGCTTC+AGG - chr4.1:73200686-73200705 MS.gene059222:intron 40.0%
! CTGAAGCTAATTCCCGATTT+TGG + chr4.1:73200688-73200707 None:intergenic 40.0%
! GAATTAGCTTCAGGTCGAAT+GGG - chr4.1:73200695-73200714 MS.gene059222:intron 40.0%
! GAGTATATGAACACTGGTCT+TGG - chr4.1:73201945-73201964 MS.gene059222:CDS 40.0%
! GATAACCTTGCCATTTGACA+CGG + chr4.1:73201986-73202005 None:intergenic 40.0%
! TACTAGGATCTTGCCTGTTT+TGG - chr4.1:73201186-73201205 MS.gene059222:intron 40.0%
! TGTCTTTTTGCAGCTGTTGT+TGG - chr4.1:73201411-73201430 MS.gene059222:CDS 40.0%
!! AAGGTTGAGGATTTGGTGTT+TGG + chr4.1:73199853-73199872 None:intergenic 40.0%
!! CTCAAGTTGATGCTCAAACA+TGG - chr4.1:73200116-73200135 MS.gene059222:CDS 40.0%
!! GTTCAAGAAAGAGCACTCAA+AGG - chr4.1:73199959-73199978 MS.gene059222:CDS 40.0%
!!! CTAGGGTTTTGTTGAGCTTA+AGG + chr4.1:73200078-73200097 None:intergenic 40.0%
!!! GAAATGCCAGTGTTTTGGTT+AGG + chr4.1:73201732-73201751 None:intergenic 40.0%
!!! GGTTTTGTTGAGCTTAAGGA+TGG + chr4.1:73200074-73200093 None:intergenic 40.0%
AACGCAATGCAACAGCTTGA+TGG + chr4.1:73201491-73201510 None:intergenic 45.0%
ACTAACAGTTGTGTACGTCC+CGG + chr4.1:73200392-73200411 None:intergenic 45.0%
ACTTACCAACAGTGGCAGAA+CGG + chr4.1:73200591-73200610 None:intergenic 45.0%
AGAAACCGTGTCAAATGGCA+AGG - chr4.1:73201978-73201997 MS.gene059222:CDS 45.0%
CCACAAGAAACCGTGTCAAA+TGG - chr4.1:73201973-73201992 MS.gene059222:CDS 45.0%
CCATTTGACACGGTTTCTTG+TGG + chr4.1:73201976-73201995 None:intergenic 45.0%
GCCCTAGAATATTGCTTCCA+TGG + chr4.1:73201831-73201850 None:intergenic 45.0%
GTAAATGGCACTTTGGTTGC+AGG + chr4.1:73202080-73202099 None:intergenic 45.0%
GTCAACACAATCACTGCACA+AGG - chr4.1:73201696-73201715 MS.gene059222:CDS 45.0%
GTTTGATTCATCCAGCTGGT+TGG - chr4.1:73201877-73201896 MS.gene059222:CDS 45.0%
TTTGCAGCTGTTGTTGGAGA+TGG - chr4.1:73201417-73201436 MS.gene059222:CDS 45.0%
! ACAAAGATGGGTTCCCAACA+TGG - chr4.1:73200293-73200312 MS.gene059222:CDS 45.0%
! AGCTTGATGGTTCTTTGCAC+CGG + chr4.1:73201478-73201497 None:intergenic 45.0%
! ATCCAGCTGGTTGGTTAGAA+TGG - chr4.1:73201886-73201905 MS.gene059222:CDS 45.0%
! CAAAGATGGGTTCCCAACAT+GGG - chr4.1:73200294-73200313 MS.gene059222:CDS 45.0%
! GGAATTAGCTTCAGGTCGAA+TGG - chr4.1:73200694-73200713 MS.gene059222:intron 45.0%
ACATTCCGTTCTGCCACTGT+TGG - chr4.1:73200583-73200602 MS.gene059222:intron 50.0%
ACCGGATCACGGGTATAATC+CGG - chr4.1:73201017-73201036 MS.gene059222:intron 50.0%
AGTGGCAGAACGGAATGTTG+TGG + chr4.1:73200581-73200600 None:intergenic 50.0%
ATGGGTTCCCAACATGGGTT+AGG - chr4.1:73200299-73200318 MS.gene059222:CDS 50.0%
CAAGCTGTTGCATTGCGTTC+CGG - chr4.1:73201492-73201511 MS.gene059222:CDS 50.0%
CTCCATTCTAACCAACCAGC+TGG + chr4.1:73201891-73201910 None:intergenic 50.0%
GGGAACCCATCTTTGTAGCT+AGG + chr4.1:73200289-73200308 None:intergenic 50.0%
GTAGTAGCAAAAGACGGGTC+CGG - chr4.1:73200370-73200389 MS.gene059222:CDS 50.0%
TAGTAGCAAAAGACGGGTCC+GGG - chr4.1:73200371-73200390 MS.gene059222:CDS 50.0%
! ATCACCGGTAGCAAAAGCGT+TGG - chr4.1:73200547-73200566 MS.gene059222:intron 50.0%
! GCTGGTTGGTTAGAATGGAG+TGG - chr4.1:73201891-73201910 MS.gene059222:CDS 50.0%
! GGCTGCTTCGGATTTGAAAC+CGG - chr4.1:73201773-73201792 MS.gene059222:CDS 50.0%
! TCCTCCAACGCTTTTGCTAC+CGG + chr4.1:73200554-73200573 None:intergenic 50.0%
!! TTCTAGGGTTACGGCTGCTT+CGG - chr4.1:73201761-73201780 MS.gene059222:CDS 50.0%
CACCTGGCCTAACCCATGTT+GGG + chr4.1:73200309-73200328 None:intergenic 55.0%
CCCGGATTATACCCGTGATC+CGG + chr4.1:73201021-73201040 None:intergenic 55.0%
CCGGATCACGGGTATAATCC+GGG - chr4.1:73201018-73201037 MS.gene059222:intron 55.0%
TCACCTGGCCTAACCCATGT+TGG + chr4.1:73200310-73200329 None:intergenic 55.0%
!! ACCGGTAGCAAAAGCGTTGG+AGG - chr4.1:73200550-73200569 MS.gene059222:intron 55.0%
!! GGTAGCAAAAGCGTTGGAGG+AGG - chr4.1:73200553-73200572 MS.gene059222:intron 55.0%
!! TTCCCAACATGGGTTAGGCC+AGG - chr4.1:73200304-73200323 MS.gene059222:CDS 55.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 73199824 73202112 73199824 ID=MS.gene059222
chr4.1 mRNA 73199824 73202112 73199824 ID=MS.gene059222.t1;Parent=MS.gene059222
chr4.1 exon 73201332 73202112 73201332 ID=MS.gene059222.t1.exon1;Parent=MS.gene059222.t1
chr4.1 CDS 73201332 73202112 73201332 ID=cds.MS.gene059222.t1;Parent=MS.gene059222.t1
chr4.1 exon 73199824 73200512 73199824 ID=MS.gene059222.t1.exon2;Parent=MS.gene059222.t1
chr4.1 CDS 73199824 73200512 73199824 ID=cds.MS.gene059222.t1;Parent=MS.gene059222.t1
Gene Sequence

>MS.gene059222

ATGAATGAAGTTAAACTTTGGTGTAACCAAACACCAAATCCTCAACCTTGTGTGTATTTCTTGAGCAGTAACCCTTCTTACCAACACAAACTCATCAAACAAAAAGTTGATTTCTTCAAACTTTCATTGGAACTTGTTCAAGAAAGAGCACTCAAAGGCCATGCAAACACTCTTTCATTAGGCTCCAAATGTAGTAACTCACGTGAAAAATCAGCTTGGGATGATTGTGTTGAGCTATATGAACAAACCATCCTTAAGCTCAACAAAACCCTAGATACTAAAACAAAGTGTTCTCAAGTTGATGCTCAAACATGGCTAAGCACAGCTCTCACCAACCTTGAGACATGTAAAGCTGGATTCTATGATCTTGGTGTTCAAGATTATATTCTTCCTCTCATGTCCAACAATGTTACTAAATTGCTAAGTAACACTTTGGCTCTTAACAAAGTTCAATATCAAGAACCTAGCTACAAAGATGGGTTCCCAACATGGGTTAGGCCAGGTGATAGAAAGCTACTACAAACTTCGTCTTCTCGACCTAACGTAGTAGTAGCAAAAGACGGGTCCGGGACGTACACAACTGTTAGTGCAGCCGTGAATGCTATACCAAAGAATAACAAAGGTAGGTATGTTATACATGTGAAGGCTGGTGTCTACAATGAACAAGTTGAGATAAAGGCAAAGAATATTATGTTGGTTGGAGATGGTATTGGTAAAACTGTAATCACCGGTAGCAAAAGCGTTGGAGGAGGAACCACAACATTCCGTTCTGCCACTGTTGGTAAGTAGTTACTGCTAAAAAAATTATTTATTGATACAAGTTTGAGATACAATATGAAATGTTGCTTATTATTAAGGTTGCCAAAATCGGGAATTAGCTTCAGGTCGAATGGGACTAGCAAAATCAAAAATCATAAAATTGCAATCAAAATCGAAAGATTTTATTCAACTACTTTTGTATAATCGATATATAGCAAAATCGAAAATCGTAATCAAAATCGATAGATTTTACCAAAACAAAAATAATGTTAAATATATGCTATGAATTTTCTAGCGTCATAAATGACTCAAAGTTTAAAGTCTACAAAATACTTATACATAAATGACTCGAAGCTTAGATGTTAAATATTCTTTAGTAGTCATAATAATACTAAACAACCTAGACAGAACTTGGTCTTTTATGTATTTATAGAACCGGATCACGGGTATAATCCGGGTCGATTGACCTATATAAAAAAAAATTGCAAGAATCATTCAATATTGACCTATATCAAAAAAATTGCAAGAATCATTCAAAATCTTGTAAAATCCTGTGATTTTGCATGGATTTCATGATTTTGTTCCATTTTTTTAATGCGGTTTTACTAGGATCTTGCCTGTTTTGGAATTCTGCAATTTATTTAAATTGTTGAAGGTGAATAAAGTTGCAAAATCCTGAATTTTTGGATTTAAGTCGGGATTTGACAACAATGCTTATTATGTCTTTAATTGTTATATATGTGTGTGTATCTAGATTAAAACATTTTCCTCTTAGTTTTTAACATTAATAGAATACACTCAATGACTAGAAATCTAACGAATATATATTGTCTTTTTGCAGCTGTTGTTGGAGATGGATTTATGGCTCAAGATATCACATTTAGGAACACTGCCGGTGCAAAGAACCATCAAGCTGTTGCATTGCGTTCCGGATCAGACTTGTCTGTATTTTATAGATGTAGTTTTGAAGGTTTTCAAGACACATTATATGTGCATTCAGATAGACAATTCTACAAACAATGTAACATTTATGGTACGGTTGATTTCATATTTGGTAATGCTGCTGCAGTTTTCCAAAATTGTAACATATTTGCAAGAAACCCTCCAAACAAAGTCAACACAATCACTGCACAAGGTAGAACAGATCCTAACCAAAACACTGGCATTTCTATTCACAATTCTAGGGTTACGGCTGCTTCGGATTTGAAACCGGTCCAAAATTCTGTTAAGACTTATCTTGGAAGACCATGGAAGCAATATTCTAGGGCAGTTTTTATGAAGACTTCTCTTGATAGTTTGATTCATCCAGCTGGTTGGTTAGAATGGAGTGGTAATTTTGCATTAAGTACTTTATATTATGGAGAGTATATGAACACTGGTCTTGGTTCTTCCACAAGAAACCGTGTCAAATGGCAAGGTTATCATGTGATAACAAGTGCTTCTGAAGCATCAAAGTTCACTGTATCAAATTTCATTGCTGGAAATGCTTGGTTACCTGCAACCAAAGTGCCATTTACATCTACTCTTTAA
Protein sequence

>MS.gene059222.t1

MNEVKLWCNQTPNPQPCVYFLSSNPSYQHKLIKQKVDFFKLSLELVQERALKGHANTLSLGSKCSNSREKSAWDDCVELYEQTILKLNKTLDTKTKCSQVDAQTWLSTALTNLETCKAGFYDLGVQDYILPLMSNNVTKLLSNTLALNKVQYQEPSYKDGFPTWVRPGDRKLLQTSSSRPNVVVAKDGSGTYTTVSAAVNAIPKNNKGRYVIHVKAGVYNEQVEIKAKNIMLVGDGIGKTVITGSKSVGGGTTTFRSATVAVVGDGFMAQDITFRNTAGAKNHQAVALRSGSDLSVFYRCSFEGFQDTLYVHSDRQFYKQCNIYGTVDFIFGNAAAVFQNCNIFARNPPNKVNTITAQGRTDPNQNTGISIHNSRVTAASDLKPVQNSVKTYLGRPWKQYSRAVFMKTSLDSLIHPAGWLEWSGNFALSTLYYGEYMNTGLGSSTRNRVKWQGYHVITSASEASKFTVSNFIAGNAWLPATKVPFTSTL