AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene48922

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene48922.t1 MTR_4g025690 97.342 489 13 0 1 489 25 513 0.0 994
MS.gene48922.t1 MTR_4g025730 74.338 491 117 5 1 489 26 509 0.0 754
MS.gene48922.t1 MTR_8g104655 50.584 514 220 10 5 489 33 541 0.0 518
MS.gene48922.t1 MTR_3g033690 53.414 498 217 9 4 489 32 526 7.61e-179 512
MS.gene48922.t1 MTR_4g035980 51.485 505 225 9 4 489 36 539 7.95e-178 510
MS.gene48922.t1 MTR_3g008640 53.307 499 217 10 4 489 32 527 1.94e-177 508
MS.gene48922.t1 MTR_3g008720 53.307 499 217 10 4 489 32 527 3.93e-177 508
MS.gene48922.t1 MTR_7g053360 53.106 499 218 10 4 489 32 527 4.82e-176 505
MS.gene48922.t1 MTR_3g008700 52.695 501 221 10 2 489 30 527 1.22e-175 504
MS.gene48922.t1 MTR_7g108905 49.012 506 228 11 8 485 49 552 1.24e-160 467
MS.gene48922.t1 MTR_8g104645 62.059 340 120 4 159 489 33 372 2.45e-155 446
MS.gene48922.t1 MTR_4g025670 52.668 431 185 8 72 489 65 489 2.01e-153 446
MS.gene48922.t1 MTR_4g087980 51.242 443 191 6 66 489 80 516 7.35e-153 446
MS.gene48922.t1 MTR_8g104580 45.224 513 216 11 5 489 25 500 1.01e-152 444
MS.gene48922.t1 MTR_8g023310 46.374 524 241 10 4 489 68 589 7.12e-149 438
MS.gene48922.t1 MTR_2g044880 50.112 447 203 5 61 489 81 525 1.37e-147 432
MS.gene48922.t1 MTR_1g008140 43.726 526 248 10 8 489 86 607 1.62e-147 435
MS.gene48922.t1 MTR_7g050980 47.852 512 229 13 6 489 54 555 1.30e-146 431
MS.gene48922.t1 MTR_1g083520 48.622 508 216 13 8 485 32 524 3.25e-145 426
MS.gene48922.t1 MTR_7g050870 51.678 447 192 9 63 489 112 554 6.75e-145 426
MS.gene48922.t1 MTR_3g091380 44.168 523 244 10 8 489 82 597 1.93e-144 427
MS.gene48922.t1 MTR_1g088020 47.566 534 218 17 8 489 32 555 2.14e-140 415
MS.gene48922.t1 MTR_7g112580 48.904 456 188 10 66 489 100 542 7.74e-139 410
MS.gene48922.t1 MTR_7g108890 46.139 531 229 15 8 489 40 562 3.89e-137 407
MS.gene48922.t1 MTR_1g054880 46.328 531 227 14 8 489 37 558 4.32e-137 407
MS.gene48922.t1 MTR_1g088225 47.378 534 217 18 8 489 32 553 4.49e-137 407
MS.gene48922.t1 MTR_7g050950 49.220 449 199 10 63 489 112 553 2.23e-136 405
MS.gene48922.t1 MTR_7g108900 45.438 537 235 15 8 489 27 560 5.71e-135 401
MS.gene48922.t1 MTR_4g087970 46.067 445 213 5 67 489 78 517 2.12e-133 396
MS.gene48922.t1 MTR_8g042910 45.772 544 224 16 8 489 78 612 2.60e-132 396
MS.gene48922.t1 MTR_8g042900 44.078 515 250 13 4 489 76 581 6.12e-132 394
MS.gene48922.t1 MTR_7g108875 43.691 531 242 12 8 489 42 564 4.19e-131 392
MS.gene48922.t1 MTR_3g010780 42.664 518 255 12 2 489 69 574 2.17e-128 385
MS.gene48922.t1 MTR_2g044810 42.200 500 267 9 1 485 38 530 1.35e-124 374
MS.gene48922.t1 MTR_4g124550 42.218 514 258 12 4 484 81 588 1.47e-123 373
MS.gene48922.t1 MTR_0458s0030 65.019 263 90 2 228 489 53 314 5.74e-122 359
MS.gene48922.t1 MTR_1g088040 65.019 263 90 2 228 489 53 314 5.74e-122 359
MS.gene48922.t1 MTR_3g010770 57.325 314 127 4 182 489 263 575 3.17e-121 367
MS.gene48922.t1 MTR_8g023150 42.442 516 257 12 4 489 77 582 3.27e-120 364
MS.gene48922.t1 MTR_4g124520 41.352 503 255 13 1 485 37 517 7.51e-120 361
MS.gene48922.t1 MTR_0618s0020 67.544 228 73 1 263 489 1 228 2.94e-116 341
MS.gene48922.t1 MTR_1g086190 40.246 487 251 11 37 485 75 559 1.04e-112 344
MS.gene48922.t1 MTR_1g086210 40.781 461 227 10 70 485 106 565 3.29e-111 341
MS.gene48922.t1 MTR_8g093460 38.803 518 268 14 2 483 41 545 2.60e-103 320
MS.gene48922.t1 MTR_2g044660 48.632 329 161 5 159 484 304 627 8.08e-103 321
MS.gene48922.t1 MTR_1g083510 37.687 536 272 16 4 487 56 581 1.18e-102 319
MS.gene48922.t1 MTR_3g095920 39.652 517 265 14 1 478 45 553 2.35e-102 318
MS.gene48922.t1 MTR_7g110490 39.530 468 226 12 70 484 106 569 1.58e-101 316
MS.gene48922.t1 MTR_7g110510 39.530 468 226 12 70 484 106 569 1.58e-101 316
MS.gene48922.t1 MTR_7g110440 37.821 468 234 10 70 484 106 569 4.88e-101 315
MS.gene48922.t1 MTR_7g112590 36.278 532 280 14 4 486 59 580 9.14e-101 314
MS.gene48922.t1 MTR_1g083470 34.915 527 286 12 1 485 55 566 8.28e-100 311
MS.gene48922.t1 MTR_1g083500 34.915 527 286 12 1 485 55 566 8.28e-100 311
MS.gene48922.t1 MTR_7g110560 37.452 518 277 13 4 485 39 545 1.41e-99 310
MS.gene48922.t1 MTR_4g005170 38.496 452 243 12 66 485 123 571 2.32e-97 307
MS.gene48922.t1 MTR_1g083490 37.719 456 249 11 66 489 123 575 2.40e-97 305
MS.gene48922.t1 MTR_4g005180 37.446 462 234 12 66 485 105 553 2.06e-95 300
MS.gene48922.t1 MTR_4g120290 36.782 522 278 15 4 485 59 568 3.86e-94 297
MS.gene48922.t1 MTR_2g436040 33.333 507 308 11 4 486 43 543 3.03e-93 295
MS.gene48922.t1 MTR_4g120280 33.966 527 288 11 4 486 59 569 1.42e-92 293
MS.gene48922.t1 MTR_2g044810 53.846 247 112 2 240 485 216 461 3.88e-92 288
MS.gene48922.t1 MTR_8g088610 44.277 332 176 7 161 486 45 373 1.57e-91 283
MS.gene48922.t1 MTR_2g436050 36.170 470 268 11 48 486 93 561 4.23e-88 281
MS.gene48922.t1 MTR_7g110420 36.478 477 242 12 70 489 106 578 8.66e-88 280
MS.gene48922.t1 MTR_5g083620 42.012 338 180 7 159 485 20 352 9.01e-87 271
MS.gene48922.t1 MTR_0458s0010 38.772 521 225 19 24 489 37 518 2.02e-84 270
MS.gene48922.t1 MTR_7g110430 33.405 467 222 10 70 484 106 535 2.56e-81 262
MS.gene48922.t1 MTR_2g105560 40.385 312 171 5 181 489 40 339 1.52e-73 236
MS.gene48922.t1 MTR_7g107180 40.317 315 175 5 178 489 3 307 2.75e-72 231
MS.gene48922.t1 MTR_6g024165 39.875 321 168 8 169 478 28 334 2.73e-67 219
MS.gene48922.t1 MTR_2g039530 42.434 304 158 6 158 456 28 319 1.27e-63 210
MS.gene48922.t1 MTR_7g111560 30.099 505 314 16 8 487 64 554 1.62e-62 213
MS.gene48922.t1 MTR_1g086540 36.646 322 178 6 169 478 37 344 3.42e-57 193
MS.gene48922.t1 MTR_8g102380 38.158 304 159 6 190 478 88 377 1.91e-55 190
MS.gene48922.t1 MTR_6g088810 39.145 304 154 7 190 478 90 377 9.53e-55 188
MS.gene48922.t1 MTR_6g012800 34.356 326 179 9 182 487 6 316 4.21e-52 179
MS.gene48922.t1 MTR_6g042660 35.764 288 157 7 171 451 30 296 1.03e-48 170
MS.gene48922.t1 MTR_7g092780 32.132 333 195 7 159 478 73 387 1.84e-47 169
MS.gene48922.t1 MTR_8g102380 40.726 248 121 6 190 422 88 324 3.48e-47 166
MS.gene48922.t1 MTR_7g011530 33.230 322 184 8 184 487 8 316 7.78e-47 165
MS.gene48922.t1 MTR_5g083590 32.143 308 155 5 182 485 7 264 2.93e-46 162
MS.gene48922.t1 MTR_5g095490 33.876 307 171 6 188 478 67 357 3.85e-45 162
MS.gene48922.t1 MTR_6g042690 34.507 284 153 8 182 451 39 303 1.62e-43 157
MS.gene48922.t1 MTR_2g033970 32.390 318 191 6 172 480 46 348 2.14e-43 157
MS.gene48922.t1 MTR_4g124740 34.091 308 170 7 182 478 54 339 2.18e-43 157
MS.gene48922.t1 MTR_7g102600 32.459 305 184 6 184 478 101 393 4.38e-43 157
MS.gene48922.t1 MTR_1g033050 32.982 285 168 5 177 451 13 284 2.53e-42 153
MS.gene48922.t1 MTR_5g012600 31.529 314 187 7 182 481 63 362 3.41e-40 149
MS.gene48922.t1 MTR_5g088300 32.215 298 183 5 182 478 37 316 9.23e-39 144
MS.gene48922.t1 MTR_5g093970 33.115 305 178 9 182 478 43 329 1.03e-38 144
MS.gene48922.t1 MTR_2g436600 31.348 319 184 9 172 480 45 338 8.25e-38 141
MS.gene48922.t1 MTR_2g073390 30.247 324 203 8 169 483 58 367 3.36e-37 140
MS.gene48922.t1 MTR_4g074290 31.193 327 196 9 169 483 58 367 6.79e-36 137
MS.gene48922.t1 MTR_4g051810 32.441 299 182 6 182 478 37 317 1.36e-35 135
MS.gene48922.t1 MTR_5g088280 31.229 301 188 5 179 478 36 318 2.65e-35 134
MS.gene48922.t1 MTR_5g088290 31.229 301 183 7 182 478 48 328 5.87e-34 131
MS.gene48922.t1 MTR_0009s0350 28.834 326 205 8 169 483 58 367 3.17e-32 127
MS.gene48922.t1 MTR_0009s0340 28.834 326 205 8 169 483 58 367 3.17e-32 127
MS.gene48922.t1 MTR_8g036225 27.302 315 200 6 182 486 33 328 2.19e-30 120
MS.gene48922.t1 MTR_6g025590 38.122 181 102 3 169 339 36 216 1.92e-29 116
MS.gene48922.t1 MTR_2g436070 27.957 279 157 10 4 251 56 321 8.46e-20 91.3
MS.gene48922.t1 MTR_4g130790 28.112 249 147 8 185 420 25 254 3.70e-16 79.0
MS.gene48922.t1 MTR_0408s0030 40.404 99 46 3 355 451 36 123 1.79e-15 74.3
MS.gene48922.t1 MTR_6g024200 34.375 160 84 5 320 478 137 276 2.78e-15 76.6
MS.gene48922.t1 MTR_7g102580 40.659 91 44 2 249 334 68 153 2.80e-12 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene48922.t1 AT2G45220 67.213 488 154 4 3 489 29 511 0.0 699
MS.gene48922.t1 AT5G51490 50.992 504 222 9 7 489 37 536 4.93e-176 505
MS.gene48922.t1 AT3G47400 49.609 512 233 9 3 489 83 594 1.53e-172 498
MS.gene48922.t1 AT4G00190 53.388 487 202 9 8 489 8 474 4.02e-172 493
MS.gene48922.t1 AT5G51500 50.598 502 227 9 7 489 41 540 6.59e-172 495
MS.gene48922.t1 AT1G23200 47.736 530 216 13 8 489 38 554 1.60e-152 446
MS.gene48922.t1 AT3G49220 45.187 509 249 8 8 489 91 596 1.59e-146 432
MS.gene48922.t1 AT3G43270 48.857 481 214 8 39 489 47 525 4.01e-146 429
MS.gene48922.t1 AT3G49220 45.187 509 249 8 8 489 136 641 1.39e-145 431
MS.gene48922.t1 AT5G53370 44.031 511 253 8 8 489 79 585 3.03e-144 426
MS.gene48922.t1 AT3G14310 49.467 469 186 10 68 486 122 589 5.23e-144 426
MS.gene48922.t1 AT3G60730 47.414 464 202 7 62 487 58 517 6.06e-144 423
MS.gene48922.t1 AT5G53370 44.031 511 253 8 8 489 115 621 1.19e-143 426
MS.gene48922.t1 AT3G59010 49.260 473 208 8 39 489 66 528 4.39e-143 421
MS.gene48922.t1 AT1G53830 45.472 530 241 11 4 489 62 587 6.90e-142 420
MS.gene48922.t1 AT4G02320 47.253 455 213 10 60 489 66 518 4.94e-140 413
MS.gene48922.t1 AT1G11580 49.658 439 197 7 68 485 118 553 6.11e-138 409
MS.gene48922.t1 AT1G11580 49.658 439 197 7 68 485 139 574 1.39e-137 409
MS.gene48922.t1 AT4G33220 44.118 510 241 9 21 489 17 523 2.20e-137 406
MS.gene48922.t1 AT3G05620 49.231 455 187 11 67 489 99 541 4.01e-135 401
MS.gene48922.t1 AT4G33220 56.627 332 141 2 161 489 71 402 6.61e-135 395
MS.gene48922.t1 AT4G02330 44.404 545 232 17 8 489 35 571 9.41e-129 386
MS.gene48922.t1 AT2G43050 48.611 432 190 10 75 489 100 516 1.79e-128 383
MS.gene48922.t1 AT4G02300 45.169 445 212 10 69 486 90 529 1.22e-126 379
MS.gene48922.t1 AT1G02810 42.523 555 237 14 8 489 32 577 3.72e-126 379
MS.gene48922.t1 AT5G04960 41.879 511 254 13 4 487 68 562 1.14e-123 372
MS.gene48922.t1 AT2G26450 43.404 470 238 11 38 484 142 606 1.65e-123 374
MS.gene48922.t1 AT2G47550 46.220 463 201 11 72 489 99 558 1.14e-121 367
MS.gene48922.t1 AT3G10720 42.251 542 244 16 8 489 83 615 1.20e-121 369
MS.gene48922.t1 AT3G10710 42.690 513 246 13 4 489 70 561 1.22e-121 367
MS.gene48922.t1 AT5G20860 40.856 514 270 12 2 489 33 538 4.43e-121 365
MS.gene48922.t1 AT1G53840 40.385 520 259 12 4 483 73 581 1.14e-119 363
MS.gene48922.t1 AT3G05610 41.501 453 233 10 66 489 116 565 2.07e-118 362
MS.gene48922.t1 AT2G26440 40.116 516 260 12 7 485 40 543 2.90e-118 358
MS.gene48922.t1 AT5G04970 41.697 542 247 17 8 489 88 620 1.79e-116 356
MS.gene48922.t1 AT5G49180 40.039 517 270 15 4 485 55 566 4.87e-116 353
MS.gene48922.t1 AT3G06830 39.425 487 255 11 39 489 86 568 5.30e-116 353
MS.gene48922.t1 AT5G27870 41.348 445 235 8 66 485 115 558 2.41e-115 356
MS.gene48922.t1 AT4G33230 42.576 458 226 11 60 484 148 601 5.06e-112 344
MS.gene48922.t1 AT3G10720 60.385 260 101 2 231 489 1 259 3.00e-110 327
MS.gene48922.t1 AT3G14300 39.567 508 260 12 8 479 463 959 7.01e-110 348
MS.gene48922.t1 AT4G15980 47.564 349 174 5 147 487 353 700 6.02e-107 333
MS.gene48922.t1 AT4G15980 47.564 349 174 5 147 487 363 710 1.40e-106 333
MS.gene48922.t1 AT3G27980 42.893 394 216 5 103 489 106 497 1.14e-104 322
MS.gene48922.t1 AT1G11590 43.000 400 215 5 101 489 127 524 2.25e-101 314
MS.gene48922.t1 AT4G03930 42.462 398 216 6 103 489 129 524 8.90e-101 312
MS.gene48922.t1 AT5G64640 39.652 517 267 17 3 481 92 601 1.09e-100 315
MS.gene48922.t1 AT5G09760 39.029 515 262 17 3 477 44 546 1.40e-99 310
MS.gene48922.t1 AT5G09760 38.835 515 263 16 3 477 70 572 2.56e-99 310
MS.gene48922.t1 AT5G20860 37.728 493 245 12 2 468 33 489 1.78e-97 303
MS.gene48922.t1 AT2G47030 44.540 348 173 6 160 489 243 588 5.47e-86 276
MS.gene48922.t1 AT2G47040 43.768 345 177 6 160 489 253 595 2.24e-82 266
MS.gene48922.t1 AT3G62170 41.737 357 180 6 154 484 229 583 2.37e-82 266
MS.gene48922.t1 AT1G11370 48.996 249 124 2 174 419 38 286 4.46e-80 251
MS.gene48922.t1 AT5G19730 37.864 309 165 6 183 478 87 381 1.52e-54 187
MS.gene48922.t1 AT3G17060 33.542 319 184 7 172 479 36 337 3.24e-50 175
MS.gene48922.t1 AT2G36700 33.775 302 181 4 184 478 44 333 6.76e-49 171
MS.gene48922.t1 AT1G05310 33.934 333 189 8 159 478 74 388 1.64e-48 172
MS.gene48922.t1 AT3G29090 35.106 282 161 4 182 451 7 278 1.01e-47 167
MS.gene48922.t1 AT2G21610 32.890 301 179 7 182 478 51 332 1.12e-45 163
MS.gene48922.t1 AT2G21610 32.890 301 179 7 182 478 56 337 1.67e-45 163
MS.gene48922.t1 AT5G07430 31.081 370 229 8 119 478 2 355 3.10e-45 162
MS.gene48922.t1 AT2G36710 32.372 312 189 7 182 483 91 390 1.49e-44 161
MS.gene48922.t1 AT5G55590 33.131 329 192 7 164 478 64 378 7.25e-43 156
MS.gene48922.t1 AT5G07420 32.075 318 192 7 169 478 54 355 2.60e-42 154
MS.gene48922.t1 AT5G47500 30.699 329 191 7 164 478 52 357 4.57e-42 154
MS.gene48922.t1 AT5G18990 33.441 311 177 7 182 478 29 323 9.45e-42 152
MS.gene48922.t1 AT2G19150 33.754 317 179 9 182 485 41 339 4.25e-41 150
MS.gene48922.t1 AT3G24130 31.348 319 185 8 182 484 34 334 5.84e-41 150
MS.gene48922.t1 AT5G55590 35.165 273 152 7 164 422 64 325 7.89e-40 147
MS.gene48922.t1 AT1G44980 41.489 188 104 2 162 343 54 241 9.88e-40 144
MS.gene48922.t1 AT5G61680 31.329 316 191 8 173 478 59 358 4.98e-39 145
MS.gene48922.t1 AT1G69940 29.141 326 206 7 162 478 46 355 7.87e-38 142
MS.gene48922.t1 AT5G26810 32.692 312 183 8 182 478 34 333 2.48e-37 140
MS.gene48922.t1 AT5G07410 29.141 326 206 7 162 478 46 355 5.17e-37 140
MS.gene48922.t1 AT2G47280 32.476 311 178 8 182 478 35 327 1.97e-34 132
MS.gene48922.t1 AT3G49220 35.193 233 121 7 68 274 194 422 4.11e-34 133
MS.gene48922.t1 AT2G19150 34.921 252 140 7 182 422 41 279 6.38e-34 129

Find 101 sgRNAs with CRISPR-Local

Find 145 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGCAAATATGTTGCAATTT+TGG 0.085785 4.3:+72867347 None:intergenic
TACAAACAATGCAACATTTA+TGG 0.116630 4.3:-72867409 MS.gene48922:CDS
GATAAGTCTTAACAGAATTT+TGG 0.160141 4.3:+72867206 None:intergenic
GCATTTCTATTCACAATTCT+AGG 0.200545 4.3:-72867258 MS.gene48922:CDS
TACCTACCTTTGTTATTCTT+TGG 0.232922 4.3:+72868573 None:intergenic
GCATTAAGTACTTTATATTA+TGG 0.242821 4.3:-72867082 MS.gene48922:CDS
CCAAAGTTTAACTTCATTCA+TGG 0.264428 4.3:+72869181 None:intergenic
TGTTGTTGGAGATGGATTTA+TGG 0.279809 4.3:-72867578 MS.gene48922:CDS
TGGCTCAAGATATCACATTT+AGG 0.292215 4.3:-72867558 MS.gene48922:CDS
ACTGTATCAAATTTCATTGC+TGG 0.309927 4.3:-72866962 MS.gene48922:CDS
TTCACGTGAGTTACTACATT+TGG 0.320043 4.3:+72868995 None:intergenic
TACACACAAGGTTGAGGATT+TGG 0.321560 4.3:+72869146 None:intergenic
GTGATTGTGTTGACTTTGTT+TGG 0.331970 4.3:+72867316 None:intergenic
TGATTTCTTCAAACTTTCAT+TGG 0.341515 4.3:-72869072 MS.gene48922:CDS
GTAAATGGCACTTTGGTTGC+AGG 0.349440 4.3:+72866926 None:intergenic
AAGGTTGAGGATTTGGTGTT+TGG 0.356796 4.3:+72869153 None:intergenic
ATCCAGATGGTTGGTTAGAA+TGG 0.359713 4.3:-72867117 MS.gene48922:CDS
AATTCTGTTAAGACTTATCT+TGG 0.359832 4.3:-72867202 MS.gene48922:CDS
AATTAAAGAGTAGATGTAAA+TGG 0.364262 4.3:+72866911 None:intergenic
CAAGCTGTTGCATTGCGTTC+CGG 0.373938 4.3:-72867511 MS.gene48922:CDS
GACCATGGAAGCAATATTCT+AGG 0.374299 4.3:-72867177 MS.gene48922:CDS
CATGCAAACACTCTTTCATT+AGG 0.385373 4.3:-72869020 MS.gene48922:CDS
CAATGAACAAGTTGAGATAA+AGG 0.386142 4.3:-72868523 MS.gene48922:CDS
TTCTAGGGTTACCGCTGCTT+CGG 0.395543 4.3:-72867242 MS.gene48922:CDS
TAGCAATTTAGTAACATTGT+TGG 0.401894 4.3:+72868779 None:intergenic
CGCTGCTTCGGATTTGAAAC+CGG 0.416213 4.3:-72867230 MS.gene48922:CDS
GGGAACCCATCTTTGTAGCT+AGG 0.418473 4.3:+72868717 None:intergenic
GAATAGAAATGCCAGTGTTT+TGG 0.434967 4.3:+72867269 None:intergenic
GCAAAGAATATTATGTTGGT+TGG 0.435785 4.3:-72868501 MS.gene48922:CDS
ATTCTTTGGTATAGCATTCA+CGG 0.447221 4.3:+72868587 None:intergenic
CCATGAATGAAGTTAAACTT+TGG 0.447619 4.3:-72869181 MS.gene48922:CDS
ACTAACAGTTGTGTACTTCC+CGG 0.449602 4.3:+72868614 None:intergenic
AACGCAATGCAACAGCTTGA+TGG 0.450785 4.3:+72867515 None:intergenic
CATTTCTATTCACAATTCTA+GGG 0.450955 4.3:-72867257 MS.gene48922:CDS
TTTGCAGCTGTTGTTGGAGA+TGG 0.451928 4.3:-72867586 MS.gene48922:intron
CTCCATTCTAACCAACCATC+TGG 0.471248 4.3:+72867115 None:intergenic
CACCTGGCCTAACCCATGTT+GGG 0.479286 4.3:+72868697 None:intergenic
AATATTATGTTGGTTGGAGA+TGG 0.480645 4.3:-72868495 MS.gene48922:CDS
ATGAGTTTGTGTTGGTAAGA+AGG 0.482647 4.3:+72869107 None:intergenic
AAAGGCAAAGAATATTATGT+TGG 0.483951 4.3:-72868505 MS.gene48922:CDS
CCATTTGACACGGTTTCTTG+TGG 0.485738 4.3:+72867030 None:intergenic
GAGTATATGAACACTGGTCT+TGG 0.488203 4.3:-72867058 MS.gene48922:CDS
TTGTGTTGACTTTGTTTGGA+GGG 0.490707 4.3:+72867320 None:intergenic
CAAGAACCTAGCTACAAAGA+TGG 0.493394 4.3:-72868723 MS.gene48922:CDS
TAGTAGCAAAAGACGGGTCC+GGG 0.495277 4.3:-72868632 MS.gene48922:CDS
ATTTCATTGCTGGAAATGCT+TGG 0.496886 4.3:-72866952 MS.gene48922:CDS
TGTAGTAGCTTTCTATCACC+TGG 0.499249 4.3:+72868681 None:intergenic
GTAGTAGCAAAAGACGGGTC+CGG 0.499299 4.3:-72868633 MS.gene48922:CDS
CCACAAGAAACCGTGTCAAA+TGG 0.501895 4.3:-72867030 MS.gene48922:CDS
GCCCTAGAATATTGCTTCCA+TGG 0.507314 4.3:+72867175 None:intergenic
TAAATTGCTAAGTAACACTT+TGG 0.507939 4.3:-72868766 MS.gene48922:CDS
AGCTTGATGGTTCTTTGCAC+CGG 0.510568 4.3:+72867528 None:intergenic
TCACCTGGCCTAACCCATGT+TGG 0.518597 4.3:+72868696 None:intergenic
TTTGTTTGATGAGTTTGTGT+TGG 0.521126 4.3:+72869099 None:intergenic
AGTAGATGTAAATGGCACTT+TGG 0.524094 4.3:+72866919 None:intergenic
ACCGGTAGCAAAAGCGTTGG+AGG 0.524747 4.3:-72868453 MS.gene48922:CDS
AAATACAGACAAGTCTGATC+CGG 0.525381 4.3:+72867492 None:intergenic
ATGTTGGTTGGAGATGGTAT+TGG 0.525732 4.3:-72868489 MS.gene48922:CDS
AAAGCTGGATTCTATGATCT+TGG 0.527056 4.3:-72868831 MS.gene48922:CDS
TTCCCAACATGGGTTAGGCC+AGG 0.531406 4.3:-72868699 MS.gene48922:CDS
AAGAAATACACACAAGGTTG+AGG 0.533464 4.3:+72869140 None:intergenic
ATGGGTTCCCAACATGGGTT+AGG 0.533950 4.3:-72868704 MS.gene48922:CDS
TGAGTTTGTGTTGGTAAGAA+GGG 0.534506 4.3:+72869108 None:intergenic
ACAAAGATGGGTTCCCAACA+TGG 0.535204 4.3:-72868710 MS.gene48922:CDS
GTTTGATTCATCCAGATGGT+TGG 0.539110 4.3:-72867126 MS.gene48922:CDS
AGCTTTACATGTCTCAAGGT+TGG 0.546562 4.3:+72868848 None:intergenic
ACAATGCAACATTTATGGTA+CGG 0.547191 4.3:-72867404 MS.gene48922:CDS
AACCTTGAGACATGTAAAGC+TGG 0.551306 4.3:-72868846 MS.gene48922:CDS
AAGAACCTAGCTACAAAGAT+GGG 0.557927 4.3:-72868722 MS.gene48922:CDS
TAGGTATGTTATACATGTGA+AGG 0.561706 4.3:-72868556 MS.gene48922:CDS
CTCAAGTTGATGCTCAAACA+TGG 0.563888 4.3:-72868887 MS.gene48922:CDS
ATCCAGCTTTACATGTCTCA+AGG 0.564983 4.3:+72868844 None:intergenic
ATTGTGTTGACTTTGTTTGG+AGG 0.571190 4.3:+72867319 None:intergenic
GATAGTTTGATTCATCCAGA+TGG 0.585344 4.3:-72867130 MS.gene48922:CDS
ACATTCCGTTCTGCCACTGT+TGG 0.587276 4.3:-72868420 MS.gene48922:intron
GATAACCTTGCCATTTGACA+CGG 0.589011 4.3:+72867020 None:intergenic
ACGTAGTAGTAGCAAAAGAC+GGG 0.589841 4.3:-72868638 MS.gene48922:CDS
AACGTAGTAGTAGCAAAAGA+CGG 0.590566 4.3:-72868639 MS.gene48922:CDS
ACCATGGAAGCAATATTCTA+GGG 0.592357 4.3:-72867176 MS.gene48922:CDS
ATTGGTAAAACTGTAATCAC+CGG 0.592601 4.3:-72868471 MS.gene48922:CDS
CTAATGAAAGAGTGTTTGCA+TGG 0.596121 4.3:+72869021 None:intergenic
AGACTTATCTTGGAAGACCA+TGG 0.599466 4.3:-72867192 MS.gene48922:CDS
ATCACCGGTAGCAAAAGCGT+TGG 0.601310 4.3:-72868456 MS.gene48922:CDS
AGAAACCGTGTCAAATGGCA+AGG 0.607462 4.3:-72867025 MS.gene48922:CDS
TACCAAAGAATAACAAAGGT+AGG 0.608433 4.3:-72868575 MS.gene48922:CDS
GGTAGCAAAAGCGTTGGAGG+AGG 0.610712 4.3:-72868450 MS.gene48922:CDS
ATCACATTTAGGAACACTGC+CGG 0.612058 4.3:-72867547 MS.gene48922:CDS
GTAACATTGTTGGACATGAG+AGG 0.612493 4.3:+72868789 None:intergenic
CAAAGATGGGTTCCCAACAT+GGG 0.625227 4.3:-72868709 MS.gene48922:CDS
ACAGATCCTAACCAAAACAC+TGG 0.626471 4.3:-72867280 MS.gene48922:CDS
GCTATACCAAAGAATAACAA+AGG 0.633072 4.3:-72868579 MS.gene48922:CDS
TATGGAGAGTATATGAACAC+TGG 0.633586 4.3:-72867064 MS.gene48922:CDS
TATGTTATACATGTGAAGGC+TGG 0.634350 4.3:-72868552 MS.gene48922:CDS
ACTTACCAACAGTGGCAGAA+CGG 0.640460 4.3:+72868415 None:intergenic
GTTCAAGAAAGAGCACTCAA+AGG 0.644742 4.3:-72869044 MS.gene48922:CDS
GTCAACACAATCACTGCACA+AGG 0.651414 4.3:-72867307 MS.gene48922:CDS
CTGCTCAAGAAATACACACA+AGG 0.658259 4.3:+72869134 None:intergenic
CGGTTTCAAATCCGAAGCAG+CGG 0.662915 4.3:+72867231 None:intergenic
GATGGTTGGTTAGAATGGAG+TGG 0.667743 4.3:-72867112 MS.gene48922:CDS
CAGTAACTACTTACCAACAG+TGG 0.681222 4.3:+72868407 None:intergenic
AGTGGCAGAACGGAATGTTG+TGG 0.699012 4.3:+72868425 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTAACATTATTTTTGTTT+TGG + chr4.3:72867928-72867947 None:intergenic 10.0%
!! CAATTTATTTAAATTGTTGA+AGG - chr4.3:72868305-72868324 MS.gene48922:intron 15.0%
!! TATTAATGTTAAAAACTAAG+AGG + chr4.3:72868440-72868459 None:intergenic 15.0%
!!! CTTGCAATTTTTTTATATAT+AGG + chr4.3:72868141-72868160 None:intergenic 15.0%
!! GCATTAAGTACTTTATATTA+TGG - chr4.3:72869010-72869029 MS.gene48922:CDS 20.0%
!!! CTTTTATGTATTTATAGAAC+CGG - chr4.3:72868088-72868107 MS.gene48922:intron 20.0%
!!! TCTTGCAATTTTTTTGATAT+AGG + chr4.3:72868180-72868199 None:intergenic 20.0%
!!! TGAAATGTTGCTTATTATTA+AGG - chr4.3:72867749-72867768 MS.gene48922:intron 20.0%
!!! TTTGTTCCATTTTTTTAATG+CGG - chr4.3:72868249-72868268 MS.gene48922:intron 20.0%
!!! TTTTATAGATGTAGTTTTGA+AGG - chr4.3:72868620-72868639 MS.gene48922:CDS 20.0%
! AAAGGCAAAGAATATTATGT+TGG - chr4.3:72867587-72867606 MS.gene48922:intron 25.0%
! AATTCTGTTAAGACTTATCT+TGG - chr4.3:72868890-72868909 MS.gene48922:CDS 25.0%
! ATTATTAAGGTTGCCAAAAT+CGG - chr4.3:72867762-72867781 MS.gene48922:intron 25.0%
! CATTTCTATTCACAATTCTA+GGG - chr4.3:72868835-72868854 MS.gene48922:CDS 25.0%
! GTAAAACCGCATTAAAAAAA+TGG + chr4.3:72868258-72868277 None:intergenic 25.0%
! TAAATTGCTAAGTAACACTT+TGG - chr4.3:72867326-72867345 MS.gene48922:CDS 25.0%
! TACAAACAATGCAACATTTA+TGG - chr4.3:72868683-72868702 MS.gene48922:CDS 25.0%
! TAGCAATTTAGTAACATTGT+TGG + chr4.3:72867316-72867335 None:intergenic 25.0%
! TGATTTCTTCAAACTTTCAT+TGG - chr4.3:72867020-72867039 MS.gene48922:CDS 25.0%
!! GATAAGTCTTAACAGAATTT+TGG + chr4.3:72868889-72868908 None:intergenic 25.0%
!! TTGCAAATATGTTGCAATTT+TGG + chr4.3:72868748-72868767 None:intergenic 25.0%
!!! AACACTTTGTTTTAGTATCT+AGG + chr4.3:72867185-72867204 None:intergenic 25.0%
!!! ACACTTTGTTTTAGTATCTA+GGG + chr4.3:72867184-72867203 None:intergenic 25.0%
!!! CTGAATTTTTGGATTTAAGT+CGG - chr4.3:72868347-72868366 MS.gene48922:intron 25.0%
!!! TCGATTTTGATTACGATTTT+CGG + chr4.3:72867896-72867915 None:intergenic 25.0%
!!! TGAATTTTTGGATTTAAGTC+GGG - chr4.3:72868348-72868367 MS.gene48922:intron 25.0%
!!! TTTTTTAATGCGGTTTTACT+AGG - chr4.3:72868259-72868278 MS.gene48922:intron 25.0%
AAATTGCAGAATTCCAAAAC+AGG + chr4.3:72868291-72868310 None:intergenic 30.0%
ACAATGCAACATTTATGGTA+CGG - chr4.3:72868688-72868707 MS.gene48922:CDS 30.0%
ACTGTATCAAATTTCATTGC+TGG - chr4.3:72869130-72869149 MS.gene48922:CDS 30.0%
ATTCTTTGGTATAGCATTCA+CGG + chr4.3:72867508-72867527 None:intergenic 30.0%
ATTGGTAAAACTGTAATCAC+CGG - chr4.3:72867621-72867640 MS.gene48922:intron 30.0%
CGACTTAAATCCAAAAATTC+AGG + chr4.3:72868349-72868368 None:intergenic 30.0%
GCAAAGAATATTATGTTGGT+TGG - chr4.3:72867591-72867610 MS.gene48922:intron 30.0%
GCATTTCTATTCACAATTCT+AGG - chr4.3:72868834-72868853 MS.gene48922:CDS 30.0%
GCTATACCAAAGAATAACAA+AGG - chr4.3:72867513-72867532 MS.gene48922:CDS 30.0%
TACCAAAGAATAACAAAGGT+AGG - chr4.3:72867517-72867536 MS.gene48922:CDS 30.0%
TAGGTATGTTATACATGTGA+AGG - chr4.3:72867536-72867555 MS.gene48922:CDS 30.0%
TTATTAAGGTTGCCAAAATC+GGG - chr4.3:72867763-72867782 MS.gene48922:intron 30.0%
TTTGTTTGATGAGTTTGTGT+TGG + chr4.3:72866996-72867015 None:intergenic 30.0%
! AATATTATGTTGGTTGGAGA+TGG - chr4.3:72867597-72867616 MS.gene48922:intron 30.0%
! CAATGAACAAGTTGAGATAA+AGG - chr4.3:72867569-72867588 MS.gene48922:CDS 30.0%
! TAAAATCCTGTGATTTTGCA+TGG - chr4.3:72868216-72868235 MS.gene48922:intron 30.0%
!! GGTACGGTTGATTTTATATT+TGG - chr4.3:72868704-72868723 MS.gene48922:CDS 30.0%
!! TACCTACCTTTGTTATTCTT+TGG + chr4.3:72867522-72867541 None:intergenic 30.0%
AAAGCTGGATTCTATGATCT+TGG - chr4.3:72867261-72867280 MS.gene48922:CDS 35.0%
AAATACAGACAAGTCTGATC+CGG + chr4.3:72868603-72868622 None:intergenic 35.0%
AACGTAGTAGTAGCAAAAGA+CGG - chr4.3:72867453-72867472 MS.gene48922:CDS 35.0%
AAGAAATACACACAAGGTTG+AGG + chr4.3:72866955-72866974 None:intergenic 35.0%
AAGAACCTAGCTACAAAGAT+GGG - chr4.3:72867370-72867389 MS.gene48922:CDS 35.0%
ACCATGGAAGCAATATTCTA+GGG - chr4.3:72868916-72868935 MS.gene48922:CDS 35.0%
AGTAGATGTAAATGGCACTT+TGG + chr4.3:72869176-72869195 None:intergenic 35.0%
ATTTCATTGCTGGAAATGCT+TGG - chr4.3:72869140-72869159 MS.gene48922:CDS 35.0%
CATGCAAACACTCTTTCATT+AGG - chr4.3:72867072-72867091 MS.gene48922:CDS 35.0%
GATAGTTTGATTCATCCAGA+TGG - chr4.3:72868962-72868981 MS.gene48922:CDS 35.0%
GTATTTATAGAACCGGATCA+CGG - chr4.3:72868095-72868114 MS.gene48922:intron 35.0%
TAAAAGACCAAGTTCAGTCT+AGG + chr4.3:72868074-72868093 None:intergenic 35.0%
TAAACAACCTAGACTGAACT+TGG - chr4.3:72868064-72868083 MS.gene48922:intron 35.0%
TATATATAGGTCAATCGACC+CGG + chr4.3:72868128-72868147 None:intergenic 35.0%
TATGTTATACATGTGAAGGC+TGG - chr4.3:72867540-72867559 MS.gene48922:CDS 35.0%
TATTTATAGAACCGGATCAC+GGG - chr4.3:72868096-72868115 MS.gene48922:intron 35.0%
TGAAATCCATGCAAAATCAC+AGG + chr4.3:72868225-72868244 None:intergenic 35.0%
TGGCTCAAGATATCACATTT+AGG - chr4.3:72868534-72868553 MS.gene48922:CDS 35.0%
TTCACGTGAGTTACTACATT+TGG + chr4.3:72867100-72867119 None:intergenic 35.0%
! ATGAGTTTGTGTTGGTAAGA+AGG + chr4.3:72866988-72867007 None:intergenic 35.0%
! CAAAATCGGGATTTAACTTC+AGG - chr4.3:72867776-72867795 MS.gene48922:intron 35.0%
! CTGAAGTTAAATCCCGATTT+TGG + chr4.3:72867778-72867797 None:intergenic 35.0%
! GAATAGAAATGCCAGTGTTT+TGG + chr4.3:72868826-72868845 None:intergenic 35.0%
! GATTTAACTTCAGGTCGAAT+GGG - chr4.3:72867785-72867804 MS.gene48922:intron 35.0%
! GTCGCAAAATCCTGAATTTT+TGG - chr4.3:72868336-72868355 MS.gene48922:intron 35.0%
! GTCTTAACAGAATTTTGGAC+CGG + chr4.3:72868884-72868903 None:intergenic 35.0%
! TATGGAGAGTATATGAACAC+TGG - chr4.3:72869028-72869047 MS.gene48922:CDS 35.0%
! TCTTTTGCTACTACTACGTT+AGG + chr4.3:72867453-72867472 None:intergenic 35.0%
! TGAGTTTGTGTTGGTAAGAA+GGG + chr4.3:72866987-72867006 None:intergenic 35.0%
! TGTTGTTGGAGATGGATTTA+TGG - chr4.3:72868514-72868533 MS.gene48922:CDS 35.0%
!! ATTGTGTTGACTTTGTTTGG+AGG + chr4.3:72868776-72868795 None:intergenic 35.0%
!! CTAATGAAAGAGTGTTTGCA+TGG + chr4.3:72867074-72867093 None:intergenic 35.0%
!! GTGATTGTGTTGACTTTGTT+TGG + chr4.3:72868779-72868798 None:intergenic 35.0%
!! TTGTGTTGACTTTGTTTGGA+GGG + chr4.3:72868775-72868794 None:intergenic 35.0%
AACCTTGAGACATGTAAAGC+TGG - chr4.3:72867246-72867265 MS.gene48922:CDS 40.0%
ACAGATCCTAACCAAAACAC+TGG - chr4.3:72868812-72868831 MS.gene48922:CDS 40.0%
ACGTAGTAGTAGCAAAAGAC+GGG - chr4.3:72867454-72867473 MS.gene48922:CDS 40.0%
ACTAACAGTTGTGTACTTCC+CGG + chr4.3:72867481-72867500 None:intergenic 40.0%
ACTCACGTGAAAAATCAGCT+TGG - chr4.3:72867109-72867128 MS.gene48922:CDS 40.0%
AGACTTATCTTGGAAGACCA+TGG - chr4.3:72868900-72868919 MS.gene48922:CDS 40.0%
AGCTTTACATGTCTCAAGGT+TGG + chr4.3:72867247-72867266 None:intergenic 40.0%
ATCACATTTAGGAACACTGC+CGG - chr4.3:72868545-72868564 MS.gene48922:CDS 40.0%
ATCCAGCTTTACATGTCTCA+AGG + chr4.3:72867251-72867270 None:intergenic 40.0%
CAAGAACCTAGCTACAAAGA+TGG - chr4.3:72867369-72867388 MS.gene48922:CDS 40.0%
CAGTAACTACTTACCAACAG+TGG + chr4.3:72867688-72867707 None:intergenic 40.0%
CTCACGTGAAAAATCAGCTT+GGG - chr4.3:72867110-72867129 MS.gene48922:CDS 40.0%
CTGCTCAAGAAATACACACA+AGG + chr4.3:72866961-72866980 None:intergenic 40.0%
GACCATGGAAGCAATATTCT+AGG - chr4.3:72868915-72868934 MS.gene48922:CDS 40.0%
GTAACATTGTTGGACATGAG+AGG + chr4.3:72867306-72867325 None:intergenic 40.0%
GTTTGATTCATCCAGATGGT+TGG - chr4.3:72868966-72868985 MS.gene48922:CDS 40.0%
TACACACAAGGTTGAGGATT+TGG + chr4.3:72866949-72866968 None:intergenic 40.0%
TGTAGTAGCTTTCTATCACC+TGG + chr4.3:72867414-72867433 None:intergenic 40.0%
! ATCCAGATGGTTGGTTAGAA+TGG - chr4.3:72868975-72868994 MS.gene48922:CDS 40.0%
! ATGTTGGTTGGAGATGGTAT+TGG - chr4.3:72867603-72867622 MS.gene48922:intron 40.0%
! CATCTAGCTTCGAGTCATTT+AGG + chr4.3:72868014-72868033 None:intergenic 40.0%
! GAGTATATGAACACTGGTCT+TGG - chr4.3:72869034-72869053 MS.gene48922:CDS 40.0%
! GATAACCTTGCCATTTGACA+CGG + chr4.3:72869075-72869094 None:intergenic 40.0%
! GGATTTAACTTCAGGTCGAA+TGG - chr4.3:72867784-72867803 MS.gene48922:intron 40.0%
! TACTAGGATCTTGCCTGTTT+TGG - chr4.3:72868275-72868294 MS.gene48922:intron 40.0%
! TGTCTTTTTGCAGCTGTTGT+TGG - chr4.3:72868500-72868519 MS.gene48922:CDS 40.0%
!! AAGGTTGAGGATTTGGTGTT+TGG + chr4.3:72866942-72866961 None:intergenic 40.0%
!! CTCAAGTTGATGCTCAAACA+TGG - chr4.3:72867205-72867224 MS.gene48922:CDS 40.0%
!! GTTCAAGAAAGAGCACTCAA+AGG - chr4.3:72867048-72867067 MS.gene48922:CDS 40.0%
!!! CTAGGGTTTTGTTGAGCTTA+AGG + chr4.3:72867167-72867186 None:intergenic 40.0%
!!! GAAATGCCAGTGTTTTGGTT+AGG + chr4.3:72868821-72868840 None:intergenic 40.0%
!!! GGTTTTGTTGAGCTTAAGGA+TGG + chr4.3:72867163-72867182 None:intergenic 40.0%
AACGCAATGCAACAGCTTGA+TGG + chr4.3:72868580-72868599 None:intergenic 45.0%
ACTTACCAACAGTGGCAGAA+CGG + chr4.3:72867680-72867699 None:intergenic 45.0%
AGAAACCGTGTCAAATGGCA+AGG - chr4.3:72869067-72869086 MS.gene48922:CDS 45.0%
CCACAAGAAACCGTGTCAAA+TGG - chr4.3:72869062-72869081 MS.gene48922:CDS 45.0%
CCATTTGACACGGTTTCTTG+TGG + chr4.3:72869065-72869084 None:intergenic 45.0%
CTCCATTCTAACCAACCATC+TGG + chr4.3:72868980-72868999 None:intergenic 45.0%
GCCCTAGAATATTGCTTCCA+TGG + chr4.3:72868920-72868939 None:intergenic 45.0%
GTAAATGGCACTTTGGTTGC+AGG + chr4.3:72869169-72869188 None:intergenic 45.0%
GTCAACACAATCACTGCACA+AGG - chr4.3:72868785-72868804 MS.gene48922:CDS 45.0%
TTTGCAGCTGTTGTTGGAGA+TGG - chr4.3:72868506-72868525 MS.gene48922:CDS 45.0%
! ACAAAGATGGGTTCCCAACA+TGG - chr4.3:72867382-72867401 MS.gene48922:CDS 45.0%
! AGCTTGATGGTTCTTTGCAC+CGG + chr4.3:72868567-72868586 None:intergenic 45.0%
! CAAAGATGGGTTCCCAACAT+GGG - chr4.3:72867383-72867402 MS.gene48922:CDS 45.0%
! GATGGTTGGTTAGAATGGAG+TGG - chr4.3:72868980-72868999 MS.gene48922:CDS 45.0%
ACATTCCGTTCTGCCACTGT+TGG - chr4.3:72867672-72867691 MS.gene48922:intron 50.0%
ACCGGATCACGGGTATAATC+CGG - chr4.3:72868106-72868125 MS.gene48922:intron 50.0%
AGTGGCAGAACGGAATGTTG+TGG + chr4.3:72867670-72867689 None:intergenic 50.0%
ATGGGTTCCCAACATGGGTT+AGG - chr4.3:72867388-72867407 MS.gene48922:CDS 50.0%
CAAGCTGTTGCATTGCGTTC+CGG - chr4.3:72868581-72868600 MS.gene48922:CDS 50.0%
CGGTTTCAAATCCGAAGCAG+CGG + chr4.3:72868864-72868883 None:intergenic 50.0%
GGGAACCCATCTTTGTAGCT+AGG + chr4.3:72867378-72867397 None:intergenic 50.0%
GTAGTAGCAAAAGACGGGTC+CGG - chr4.3:72867459-72867478 MS.gene48922:CDS 50.0%
TAGTAGCAAAAGACGGGTCC+GGG - chr4.3:72867460-72867479 MS.gene48922:CDS 50.0%
! ATCACCGGTAGCAAAAGCGT+TGG - chr4.3:72867636-72867655 MS.gene48922:intron 50.0%
! CGCTGCTTCGGATTTGAAAC+CGG - chr4.3:72868862-72868881 MS.gene48922:CDS 50.0%
! TCCTCCAACGCTTTTGCTAC+CGG + chr4.3:72867643-72867662 None:intergenic 50.0%
!! TTCTAGGGTTACCGCTGCTT+CGG - chr4.3:72868850-72868869 MS.gene48922:CDS 50.0%
CACCTGGCCTAACCCATGTT+GGG + chr4.3:72867398-72867417 None:intergenic 55.0%
CCCGGATTATACCCGTGATC+CGG + chr4.3:72868110-72868129 None:intergenic 55.0%
CCGGATCACGGGTATAATCC+GGG - chr4.3:72868107-72868126 MS.gene48922:intron 55.0%
TCACCTGGCCTAACCCATGT+TGG + chr4.3:72867399-72867418 None:intergenic 55.0%
!! ACCGGTAGCAAAAGCGTTGG+AGG - chr4.3:72867639-72867658 MS.gene48922:intron 55.0%
!! GGTAGCAAAAGCGTTGGAGG+AGG - chr4.3:72867642-72867661 MS.gene48922:intron 55.0%
!! TTCCCAACATGGGTTAGGCC+AGG - chr4.3:72867393-72867412 MS.gene48922:CDS 55.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 72866913 72869201 72866913 ID=MS.gene48922
chr4.3 mRNA 72866913 72869201 72866913 ID=MS.gene48922.t1;Parent=MS.gene48922
chr4.3 exon 72868421 72869201 72868421 ID=MS.gene48922.t1.exon1;Parent=MS.gene48922.t1
chr4.3 CDS 72868421 72869201 72868421 ID=cds.MS.gene48922.t1;Parent=MS.gene48922.t1
chr4.3 exon 72866913 72867601 72866913 ID=MS.gene48922.t1.exon2;Parent=MS.gene48922.t1
chr4.3 CDS 72866913 72867601 72866913 ID=cds.MS.gene48922.t1;Parent=MS.gene48922.t1
Gene Sequence

>MS.gene48922

ATGAATGAAGTTAAACTTTGGTGTAACCAAACACCAAATCCTCAACCTTGTGTGTATTTCTTGAGCAGTAACCCTTCTTACCAACACAAACTCATCAAACAAAAAGTTGATTTCTTCAAACTTTCATTGGAACTTGTTCAAGAAAGAGCACTCAAAGGCCATGCAAACACTCTTTCATTAGGCTCCAAATGTAGTAACTCACGTGAAAAATCAGCTTGGGATGATTGTGTTGAGCTATATGAACAAACCATCCTTAAGCTCAACAAAACCCTAGATACTAAAACAAAGTGTTCTCAAGTTGATGCTCAAACATGGCTAAGCACAGCTCTCACCAACCTTGAGACATGTAAAGCTGGATTCTATGATCTTGGTGTTCAAGATTATATTCTTCCTCTCATGTCCAACAATGTTACTAAATTGCTAAGTAACACTTTGGCTCTTAACAAAGTTCAATATCAAGAACCTAGCTACAAAGATGGGTTCCCAACATGGGTTAGGCCAGGTGATAGAAAGCTACTACAAACTTCGTCTTCTCGACCTAACGTAGTAGTAGCAAAAGACGGGTCCGGGAAGTACACAACTGTTAGTGCAGCCGTGAATGCTATACCAAAGAATAACAAAGGTAGGTATGTTATACATGTGAAGGCTGGTGTCTACAATGAACAAGTTGAGATAAAGGCAAAGAATATTATGTTGGTTGGAGATGGTATTGGTAAAACTGTAATCACCGGTAGCAAAAGCGTTGGAGGAGGAACCACAACATTCCGTTCTGCCACTGTTGGTAAGTAGTTACTGCTAAAAAAAATTATTTATTGATACAAGTTTGAGATACAATATGAAATGTTGCTTATTATTAAGGTTGCCAAAATCGGGATTTAACTTCAGGTCGAATGGGACTAGCAAAATCAAAAATCATAAAATTGCAATCAAAATCGAAAGATTTTACTCAACTACTTTTGTATAATCGATATATAGAAAAACCGAAAATCGTAATCAAAATCGATAGATTTTACCAAAACAAAAATAATGTTAAATATATGCTATGAATTTTCTAGAGTCATAAATGACTCAAAGTTTAAAGTCTACAAAATACTTATACCTAAATGACTCGAAGCTAGATGTCAAATATTCTTTAGTAGTCATAATAATACTAAACAACCTAGACTGAACTTGGTCTTTTATGTATTTATAGAACCGGATCACGGGTATAATCCGGGTCGATTGACCTATATATAAAAAAATTGCAAGAATCATTCAATATTGACCTATATCAAAAAAATTGCAAGAATCATTCAAAATCTTGTAAAATCCTGTGATTTTGCATGGATTTCATGATTTTGTTCCATTTTTTTAATGCGGTTTTACTAGGATCTTGCCTGTTTTGGAATTCTGCAATTTATTTAAATTGTTGAAGGTGAATAAAGTCGCAAAATCCTGAATTTTTGGATTTAAGTCGGGATTTGACAACAATGCTTATTATGTCTTTAATTGTTATATATGTGTGTATCTAGATTAAAACATTCTCCTCTTAGTTTTTAACATTAATAGAATACACTCAATGACTAGAAATCTAACGAATATATATTATGTCTTTTTGCAGCTGTTGTTGGAGATGGATTTATGGCTCAAGATATCACATTTAGGAACACTGCCGGTGCAAAGAACCATCAAGCTGTTGCATTGCGTTCCGGATCAGACTTGTCTGTATTTTATAGATGTAGTTTTGAAGGTTTTCAAGACACATTATATGTGCATTCAGATAGACAATTCTACAAACAATGCAACATTTATGGTACGGTTGATTTTATATTTGGCAATGCTGCTGCAGTGTTCCAAAATTGCAACATATTTGCAAGAAACCCTCCAAACAAAGTCAACACAATCACTGCACAAGGTAGAACAGATCCTAACCAAAACACTGGCATTTCTATTCACAATTCTAGGGTTACCGCTGCTTCGGATTTGAAACCGGTCCAAAATTCTGTTAAGACTTATCTTGGAAGACCATGGAAGCAATATTCTAGGGCAGTTTTTATGAAGACTTCTCTTGATAGTTTGATTCATCCAGATGGTTGGTTAGAATGGAGTGGTAATTTTGCATTAAGTACTTTATATTATGGAGAGTATATGAACACTGGTCTTGGTTCTTCCACAAGAAACCGTGTCAAATGGCAAGGTTATCATGTGATAACAAGTGCTTCTGAAGCATCAAAGTTCACTGTATCAAATTTCATTGCTGGAAATGCTTGGTTACCTGCAACCAAAGTGCCATTTACATCTACTCTTTAA
Protein sequence

>MS.gene48922.t1

MNEVKLWCNQTPNPQPCVYFLSSNPSYQHKLIKQKVDFFKLSLELVQERALKGHANTLSLGSKCSNSREKSAWDDCVELYEQTILKLNKTLDTKTKCSQVDAQTWLSTALTNLETCKAGFYDLGVQDYILPLMSNNVTKLLSNTLALNKVQYQEPSYKDGFPTWVRPGDRKLLQTSSSRPNVVVAKDGSGKYTTVSAAVNAIPKNNKGRYVIHVKAGVYNEQVEIKAKNIMLVGDGIGKTVITGSKSVGGGTTTFRSATVAVVGDGFMAQDITFRNTAGAKNHQAVALRSGSDLSVFYRCSFEGFQDTLYVHSDRQFYKQCNIYGTVDFIFGNAAAVFQNCNIFARNPPNKVNTITAQGRTDPNQNTGISIHNSRVTAASDLKPVQNSVKTYLGRPWKQYSRAVFMKTSLDSLIHPDGWLEWSGNFALSTLYYGEYMNTGLGSSTRNRVKWQGYHVITSASEASKFTVSNFIAGNAWLPATKVPFTSTL