AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene45853

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene45853.t1 MTR_4g025690 97.546 489 12 0 1 489 25 513 0.0 997
MS.gene45853.t1 MTR_4g025730 74.949 491 114 5 1 489 26 509 0.0 761
MS.gene45853.t1 MTR_8g104655 50.584 514 220 10 5 489 33 541 3.59e-180 516
MS.gene45853.t1 MTR_3g033690 53.493 501 212 10 4 489 32 526 1.14e-179 514
MS.gene45853.t1 MTR_3g008640 53.386 502 212 11 4 489 32 527 3.62e-178 510
MS.gene45853.t1 MTR_4g035980 51.485 505 225 9 4 489 36 539 4.27e-178 511
MS.gene45853.t1 MTR_3g008720 53.386 502 212 11 4 489 32 527 6.73e-178 509
MS.gene45853.t1 MTR_7g053360 53.386 502 212 11 4 489 32 527 1.96e-177 508
MS.gene45853.t1 MTR_3g008700 52.778 504 216 11 2 489 30 527 2.22e-176 506
MS.gene45853.t1 MTR_7g108905 53.499 443 180 8 69 485 110 552 1.89e-160 466
MS.gene45853.t1 MTR_8g104645 62.059 340 120 4 159 489 33 372 4.14e-155 446
MS.gene45853.t1 MTR_4g025670 53.052 426 181 8 72 484 65 484 7.02e-154 447
MS.gene45853.t1 MTR_4g087980 51.242 443 191 6 66 489 80 516 8.65e-153 445
MS.gene45853.t1 MTR_8g104580 45.224 513 216 11 5 489 25 500 8.35e-152 442
MS.gene45853.t1 MTR_8g023310 45.992 524 243 10 4 489 68 589 3.58e-148 436
MS.gene45853.t1 MTR_1g008140 43.726 526 248 10 8 489 86 607 2.27e-147 435
MS.gene45853.t1 MTR_2g044880 50.112 447 203 5 61 489 81 525 2.33e-147 432
MS.gene45853.t1 MTR_7g050980 47.656 512 230 13 6 489 54 555 7.73e-147 431
MS.gene45853.t1 MTR_7g050870 51.902 447 191 9 63 489 112 554 1.53e-145 428
MS.gene45853.t1 MTR_1g083520 48.622 508 216 13 8 485 32 524 4.03e-145 426
MS.gene45853.t1 MTR_3g091380 44.168 523 244 10 8 489 82 597 7.11e-145 428
MS.gene45853.t1 MTR_1g088020 47.940 534 216 17 8 489 32 555 2.50e-141 417
MS.gene45853.t1 MTR_7g112580 48.904 456 188 10 66 489 100 542 1.82e-138 410
MS.gene45853.t1 MTR_1g088225 47.753 534 215 18 8 489 32 553 4.28e-138 409
MS.gene45853.t1 MTR_7g108890 46.328 531 228 15 8 489 40 562 1.23e-137 408
MS.gene45853.t1 MTR_1g054880 49.892 465 184 12 72 489 96 558 7.18e-137 406
MS.gene45853.t1 MTR_7g050950 49.220 449 199 10 63 489 112 553 2.44e-136 405
MS.gene45853.t1 MTR_7g108900 46.346 520 221 15 25 489 44 560 1.18e-135 403
MS.gene45853.t1 MTR_4g087970 46.067 445 213 5 67 489 78 517 3.13e-133 395
MS.gene45853.t1 MTR_7g108875 44.090 533 237 13 8 489 42 564 5.73e-132 394
MS.gene45853.t1 MTR_8g042910 46.784 513 209 14 32 489 109 612 2.04e-131 394
MS.gene45853.t1 MTR_8g042900 45.436 482 227 12 37 489 107 581 2.72e-131 392
MS.gene45853.t1 MTR_3g010780 43.827 486 233 11 34 489 99 574 2.37e-127 382
MS.gene45853.t1 MTR_2g044810 42.000 500 268 9 1 485 38 530 3.93e-124 373
MS.gene45853.t1 MTR_4g124550 42.412 514 257 12 4 484 81 588 9.67e-124 374
MS.gene45853.t1 MTR_0458s0030 65.399 263 89 2 228 489 53 314 6.32e-123 362
MS.gene45853.t1 MTR_1g088040 65.399 263 89 2 228 489 53 314 6.32e-123 362
MS.gene45853.t1 MTR_3g010770 57.325 314 127 4 182 489 263 575 1.38e-121 367
MS.gene45853.t1 MTR_8g023150 42.054 516 259 12 4 489 77 582 4.57e-120 364
MS.gene45853.t1 MTR_4g124520 47.030 404 188 10 100 485 122 517 1.05e-119 361
MS.gene45853.t1 MTR_0618s0020 67.544 228 73 1 263 489 1 228 5.53e-116 341
MS.gene45853.t1 MTR_1g086190 39.836 487 253 11 37 485 75 559 2.49e-110 338
MS.gene45853.t1 MTR_1g086210 42.317 423 205 8 101 485 144 565 9.11e-110 337
MS.gene45853.t1 MTR_8g093460 38.996 518 267 14 2 483 41 545 6.80e-104 322
MS.gene45853.t1 MTR_2g044660 48.632 329 161 5 159 484 304 627 1.31e-102 320
MS.gene45853.t1 MTR_3g095920 39.265 517 267 14 1 478 45 553 5.20e-102 317
MS.gene45853.t1 MTR_1g083510 38.200 500 257 14 37 487 85 581 2.77e-100 313
MS.gene45853.t1 MTR_7g110490 39.316 468 227 12 70 484 106 569 5.14e-100 312
MS.gene45853.t1 MTR_7g110510 39.316 468 227 12 70 484 106 569 5.14e-100 312
MS.gene45853.t1 MTR_7g112590 36.905 504 263 13 32 486 83 580 1.79e-99 311
MS.gene45853.t1 MTR_7g110440 37.607 468 235 10 70 484 106 569 2.76e-99 310
MS.gene45853.t1 MTR_7g110560 38.936 470 251 11 48 485 80 545 1.28e-98 308
MS.gene45853.t1 MTR_1g083470 37.662 462 233 11 66 485 118 566 2.78e-97 305
MS.gene45853.t1 MTR_1g083500 37.662 462 233 11 66 485 118 566 2.78e-97 305
MS.gene45853.t1 MTR_1g083490 37.281 456 251 11 66 489 123 575 2.81e-95 300
MS.gene45853.t1 MTR_4g005170 37.832 452 246 11 66 485 123 571 3.14e-95 301
MS.gene45853.t1 MTR_4g005180 37.013 462 236 12 66 485 105 553 2.57e-93 294
MS.gene45853.t1 MTR_2g044810 53.846 247 112 2 240 485 216 461 2.25e-92 289
MS.gene45853.t1 MTR_2g436040 33.136 507 309 10 4 486 43 543 2.83e-92 293
MS.gene45853.t1 MTR_4g120290 36.660 521 280 16 4 485 59 568 7.64e-92 291
MS.gene45853.t1 MTR_4g120280 33.776 527 289 11 4 486 59 569 1.48e-90 288
MS.gene45853.t1 MTR_8g088610 43.976 332 177 7 161 486 45 373 2.37e-90 281
MS.gene45853.t1 MTR_2g436050 36.170 470 268 11 48 486 93 561 2.32e-87 279
MS.gene45853.t1 MTR_5g083620 41.716 338 181 7 159 485 20 352 1.62e-85 268
MS.gene45853.t1 MTR_7g110420 36.059 477 244 12 70 489 106 578 1.62e-85 275
MS.gene45853.t1 MTR_0458s0010 39.212 533 227 20 8 489 32 518 2.99e-85 272
MS.gene45853.t1 MTR_7g110430 32.976 467 224 10 70 484 106 535 4.83e-79 256
MS.gene45853.t1 MTR_2g105560 40.385 312 171 5 181 489 40 339 1.15e-73 236
MS.gene45853.t1 MTR_7g107180 40.317 315 175 5 178 489 3 307 1.98e-72 232
MS.gene45853.t1 MTR_6g024165 39.875 321 168 8 169 478 28 334 2.76e-67 219
MS.gene45853.t1 MTR_2g039530 42.574 303 157 6 158 455 28 318 7.98e-64 211
MS.gene45853.t1 MTR_7g111560 30.099 505 314 16 8 487 64 554 1.27e-62 213
MS.gene45853.t1 MTR_1g086540 36.646 322 178 6 169 478 37 344 3.11e-57 193
MS.gene45853.t1 MTR_8g102380 38.158 304 159 6 190 478 88 377 2.38e-55 189
MS.gene45853.t1 MTR_6g088810 39.145 304 154 7 190 478 90 377 8.23e-55 188
MS.gene45853.t1 MTR_6g012800 34.663 326 178 9 182 487 6 316 5.55e-53 181
MS.gene45853.t1 MTR_6g042660 35.764 288 157 7 171 451 30 296 8.90e-49 171
MS.gene45853.t1 MTR_7g011530 33.230 322 184 8 184 487 8 316 3.25e-47 166
MS.gene45853.t1 MTR_7g092780 32.132 333 195 7 159 478 73 387 4.16e-47 168
MS.gene45853.t1 MTR_8g102380 40.726 248 121 6 190 422 88 324 5.16e-47 166
MS.gene45853.t1 MTR_5g083590 32.143 308 155 5 182 485 7 264 2.87e-46 162
MS.gene45853.t1 MTR_5g095490 33.876 307 171 6 188 478 67 357 7.15e-45 161
MS.gene45853.t1 MTR_6g042690 34.507 284 153 8 182 451 39 303 8.47e-44 157
MS.gene45853.t1 MTR_4g124740 34.091 308 170 7 182 478 54 339 1.63e-43 157
MS.gene45853.t1 MTR_2g033970 32.390 318 191 6 172 480 46 348 4.31e-43 156
MS.gene45853.t1 MTR_7g102600 32.459 305 184 6 184 478 101 393 1.01e-42 156
MS.gene45853.t1 MTR_1g033050 32.982 285 168 5 177 451 13 284 1.73e-42 153
MS.gene45853.t1 MTR_5g012600 31.529 314 187 7 182 481 63 362 1.62e-40 149
MS.gene45853.t1 MTR_5g088300 32.215 298 183 5 182 478 37 316 7.00e-39 144
MS.gene45853.t1 MTR_5g093970 33.115 305 178 9 182 478 43 329 1.07e-38 144
MS.gene45853.t1 MTR_2g436600 31.348 319 184 9 172 480 45 338 2.02e-37 140
MS.gene45853.t1 MTR_2g073390 30.247 324 203 8 169 483 58 367 3.23e-37 140
MS.gene45853.t1 MTR_4g074290 31.193 327 196 9 169 483 58 367 6.52e-36 137
MS.gene45853.t1 MTR_5g088280 31.229 301 188 5 179 478 36 318 1.29e-35 135
MS.gene45853.t1 MTR_4g051810 32.441 299 182 6 182 478 37 317 1.51e-35 135
MS.gene45853.t1 MTR_5g088290 31.229 301 183 7 182 478 48 328 2.45e-34 132
MS.gene45853.t1 MTR_0009s0350 28.834 326 205 8 169 483 58 367 2.89e-32 127
MS.gene45853.t1 MTR_0009s0340 28.834 326 205 8 169 483 58 367 2.89e-32 127
MS.gene45853.t1 MTR_8g036225 27.302 315 200 6 182 486 33 328 4.56e-30 120
MS.gene45853.t1 MTR_6g025590 38.122 181 102 3 169 339 36 216 1.81e-29 116
MS.gene45853.t1 MTR_2g436070 28.315 279 156 10 4 251 56 321 2.79e-20 92.8
MS.gene45853.t1 MTR_4g130790 28.112 249 147 8 185 420 25 254 7.64e-16 77.8
MS.gene45853.t1 MTR_0408s0030 40.404 99 46 3 355 451 36 123 1.00e-15 74.7
MS.gene45853.t1 MTR_6g024200 34.375 160 84 5 320 478 137 276 2.39e-15 76.6
MS.gene45853.t1 MTR_7g102580 40.659 91 44 2 249 334 68 153 2.74e-12 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene45853.t1 AT2G45220 67.213 488 154 4 3 489 29 511 0.0 699
MS.gene45853.t1 AT5G51490 51.389 504 220 9 7 489 37 536 2.48e-178 511
MS.gene45853.t1 AT5G51500 50.797 502 226 9 7 489 41 540 2.80e-173 498
MS.gene45853.t1 AT3G47400 49.414 512 234 9 3 489 83 594 3.17e-172 498
MS.gene45853.t1 AT4G00190 53.388 487 202 9 8 489 8 474 1.18e-171 492
MS.gene45853.t1 AT1G23200 47.925 530 215 13 8 489 38 554 3.54e-154 450
MS.gene45853.t1 AT3G43270 48.857 481 214 8 39 489 47 525 2.90e-147 432
MS.gene45853.t1 AT3G49220 44.990 509 250 8 8 489 91 596 1.55e-145 430
MS.gene45853.t1 AT3G49220 44.990 509 250 8 8 489 136 641 9.03e-145 429
MS.gene45853.t1 AT3G60730 47.629 464 201 8 62 487 58 517 2.93e-144 424
MS.gene45853.t1 AT3G14310 48.504 468 192 8 68 486 122 589 2.07e-143 424
MS.gene45853.t1 AT5G53370 43.836 511 254 8 8 489 79 585 2.54e-143 424
MS.gene45853.t1 AT3G59010 49.260 473 208 8 39 489 66 528 4.59e-143 421
MS.gene45853.t1 AT5G53370 43.836 511 254 8 8 489 115 621 6.57e-143 424
MS.gene45853.t1 AT1G53830 48.596 463 197 7 68 489 125 587 3.28e-141 418
MS.gene45853.t1 AT1G11580 50.114 439 195 7 68 485 118 553 4.47e-140 414
MS.gene45853.t1 AT4G02320 47.253 455 213 10 60 489 66 518 5.57e-140 413
MS.gene45853.t1 AT1G11580 50.114 439 195 7 68 485 139 574 1.35e-139 414
MS.gene45853.t1 AT4G33220 46.121 464 208 6 66 489 62 523 3.40e-137 406
MS.gene45853.t1 AT4G33220 56.627 332 141 2 161 489 71 402 9.67e-135 395
MS.gene45853.t1 AT3G05620 49.231 455 187 11 67 489 99 541 1.09e-134 400
MS.gene45853.t1 AT4G02330 43.670 545 236 18 8 489 35 571 4.18e-129 387
MS.gene45853.t1 AT2G43050 48.611 432 190 10 75 489 100 516 8.48e-129 384
MS.gene45853.t1 AT4G02300 45.393 445 211 10 69 486 90 529 1.83e-127 381
MS.gene45853.t1 AT1G02810 42.523 555 237 14 8 489 32 577 4.23e-126 379
MS.gene45853.t1 AT2G26450 41.865 504 264 12 4 484 109 606 2.97e-124 375
MS.gene45853.t1 AT5G04960 41.683 511 255 13 4 487 68 562 1.76e-123 372
MS.gene45853.t1 AT3G10720 42.435 542 243 16 8 489 83 615 3.67e-123 373
MS.gene45853.t1 AT2G47550 42.373 531 246 13 8 489 39 558 5.82e-122 368
MS.gene45853.t1 AT3G10710 42.495 513 247 13 4 489 70 561 4.00e-121 366
MS.gene45853.t1 AT5G20860 40.661 514 271 12 2 489 33 538 9.64e-121 364
MS.gene45853.t1 AT1G53840 40.192 520 260 12 4 483 73 581 7.43e-120 363
MS.gene45853.t1 AT2G26440 39.922 516 261 12 7 485 40 543 5.79e-118 357
MS.gene45853.t1 AT3G05610 41.280 453 234 10 66 489 116 565 3.64e-117 359
MS.gene45853.t1 AT5G04970 41.513 542 248 17 8 489 88 620 6.37e-117 357
MS.gene45853.t1 AT3G06830 39.630 487 254 11 39 489 86 568 8.78e-117 355
MS.gene45853.t1 AT5G49180 41.803 488 246 14 34 485 81 566 1.75e-116 354
MS.gene45853.t1 AT5G27870 41.124 445 236 8 66 485 115 558 8.28e-114 352
MS.gene45853.t1 AT4G33230 42.795 458 225 11 60 484 148 601 1.19e-113 348
MS.gene45853.t1 AT3G10720 60.385 260 101 2 231 489 1 259 9.48e-111 329
MS.gene45853.t1 AT3G14300 39.567 508 260 12 8 479 463 959 1.23e-110 350
MS.gene45853.t1 AT4G15980 47.564 349 174 5 147 487 353 700 1.26e-106 333
MS.gene45853.t1 AT4G15980 47.564 349 174 5 147 487 363 710 2.89e-106 332
MS.gene45853.t1 AT3G27980 43.147 394 215 5 103 489 106 497 1.46e-105 324
MS.gene45853.t1 AT1G11590 43.250 400 214 5 101 489 127 524 3.33e-102 316
MS.gene45853.t1 AT4G03930 42.714 398 215 6 103 489 129 524 7.94e-102 315
MS.gene45853.t1 AT5G64640 39.072 517 270 17 3 481 92 601 9.46e-100 312
MS.gene45853.t1 AT5G09760 38.641 515 264 17 3 477 44 546 1.65e-97 305
MS.gene45853.t1 AT5G20860 37.525 493 246 12 2 468 33 489 2.74e-97 303
MS.gene45853.t1 AT5G09760 38.447 515 265 16 3 477 70 572 2.92e-97 305
MS.gene45853.t1 AT2G47030 44.540 348 173 6 160 489 243 588 1.67e-85 275
MS.gene45853.t1 AT3G62170 41.737 357 180 6 154 484 229 583 6.54e-82 265
MS.gene45853.t1 AT2G47040 43.768 345 177 6 160 489 253 595 6.87e-82 265
MS.gene45853.t1 AT1G11370 49.398 249 123 2 174 419 38 286 4.47e-81 253
MS.gene45853.t1 AT5G19730 37.864 309 165 6 183 478 87 381 1.22e-54 188
MS.gene45853.t1 AT3G17060 33.229 319 185 7 172 479 36 337 4.39e-49 172
MS.gene45853.t1 AT1G05310 33.934 333 189 8 159 478 74 388 2.61e-48 171
MS.gene45853.t1 AT3G29090 35.106 282 161 4 182 451 7 278 3.88e-48 169
MS.gene45853.t1 AT2G36700 33.444 302 182 4 184 478 44 333 9.61e-48 168
MS.gene45853.t1 AT2G21610 32.890 301 179 7 182 478 51 332 1.22e-45 163
MS.gene45853.t1 AT2G21610 32.890 301 179 7 182 478 56 337 1.60e-45 163
MS.gene45853.t1 AT5G07430 31.081 370 229 8 119 478 2 355 2.44e-45 162
MS.gene45853.t1 AT2G36710 32.372 312 189 7 182 483 91 390 2.62e-44 161
MS.gene45853.t1 AT5G55590 33.739 329 190 8 164 478 64 378 2.23e-43 157
MS.gene45853.t1 AT5G07420 32.075 318 192 7 169 478 54 355 2.05e-42 154
MS.gene45853.t1 AT5G47500 30.699 329 191 7 164 478 52 357 2.65e-42 154
MS.gene45853.t1 AT5G18990 33.441 311 177 7 182 478 29 323 4.32e-42 153
MS.gene45853.t1 AT2G19150 33.754 317 179 9 182 485 41 339 1.57e-41 152
MS.gene45853.t1 AT3G24130 31.348 319 185 8 182 484 34 334 1.17e-40 149
MS.gene45853.t1 AT5G55590 35.165 273 152 7 164 422 64 325 2.20e-40 148
MS.gene45853.t1 AT1G44980 41.489 188 104 2 162 343 54 241 9.19e-40 144
MS.gene45853.t1 AT5G61680 31.329 316 191 8 173 478 59 358 2.91e-39 146
MS.gene45853.t1 AT1G69940 29.141 326 206 7 162 478 46 355 5.69e-38 142
MS.gene45853.t1 AT5G26810 32.692 312 183 8 182 478 34 333 4.29e-37 139
MS.gene45853.t1 AT5G07410 29.141 326 206 7 162 478 46 355 4.44e-37 140
MS.gene45853.t1 AT2G47280 32.476 311 178 8 182 478 35 327 8.05e-35 133
MS.gene45853.t1 AT2G19150 34.921 252 140 7 182 422 41 279 2.99e-34 130
MS.gene45853.t1 AT3G49220 35.780 218 113 6 68 259 194 410 4.96e-33 130

Find 100 sgRNAs with CRISPR-Local

Find 145 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGCAAATATGTTGCAATTT+TGG 0.085785 4.4:+75902889 None:intergenic
TACAAACAATGCAACATTTA+TGG 0.116630 4.4:-75902951 MS.gene45853:CDS
GATAAGTCTTAACAGAATTT+TGG 0.160141 4.4:+75902748 None:intergenic
GCATTTCTATTCACAATTCT+AGG 0.200545 4.4:-75902800 MS.gene45853:CDS
TACCTACCTTTGTTATTCTT+TGG 0.232922 4.4:+75904118 None:intergenic
GCATTAAGTACTTTATATTA+TGG 0.242821 4.4:-75902624 MS.gene45853:CDS
CCAAAGTTTAACTTCGTTCA+TGG 0.261322 4.4:+75904726 None:intergenic
TGTTGTTGGAGATGGATTTA+TGG 0.279809 4.4:-75903120 MS.gene45853:CDS
TGGCTCAAGATATCACATTT+AGG 0.292215 4.4:-75903100 MS.gene45853:CDS
ACTGTATCAAATTTCATTGC+TGG 0.309927 4.4:-75902504 MS.gene45853:CDS
TTCACGTGAGTTACTACATT+TGG 0.320043 4.4:+75904540 None:intergenic
GTGATTGTGTTGACTTTGTT+TGG 0.331970 4.4:+75902858 None:intergenic
TGATTTCTTCAAACTTTCAT+TGG 0.341515 4.4:-75904617 MS.gene45853:CDS
GTAAATGGCACTTTGGTTGC+AGG 0.349440 4.4:+75902468 None:intergenic
AATTCTGTTAAGACTTATCT+TGG 0.359832 4.4:-75902744 MS.gene45853:CDS
ATCCAGCTGGTTGGTTAGAA+TGG 0.361304 4.4:-75902659 MS.gene45853:CDS
AATTAAAGAGTAGATGTAAA+TGG 0.364262 4.4:+75902453 None:intergenic
AAGGTTGAGGATTAGGTGTT+TGG 0.366280 4.4:+75904698 None:intergenic
CAAGCTGTTGCATTGCGTTC+CGG 0.373938 4.4:-75903053 MS.gene45853:CDS
GACCATGGAAGCAATATTCT+AGG 0.374299 4.4:-75902719 MS.gene45853:CDS
CATGCAAACACTCTTTCATT+AGG 0.385373 4.4:-75904565 MS.gene45853:CDS
CAATGAACAAGTTGAGATAA+AGG 0.386142 4.4:-75904068 MS.gene45853:CDS
TTCTAGGGTTACCGCTGCTT+CGG 0.395543 4.4:-75902784 MS.gene45853:CDS
TAGCAATTTAGTAACATTGT+TGG 0.401894 4.4:+75904324 None:intergenic
CGCTGCTTCGGATTTGAAAC+CGG 0.416213 4.4:-75902772 MS.gene45853:CDS
TACACACAAGGTTGAGGATT+AGG 0.417987 4.4:+75904691 None:intergenic
GGGAACCCATCTTTGTAGCT+AGG 0.418473 4.4:+75904262 None:intergenic
AACGCAATGCAACAGCTTGA+TGG 0.429138 4.4:+75903057 None:intergenic
GAATAGAAATGCCAGTGTTT+TGG 0.434967 4.4:+75902811 None:intergenic
GCAAAGAATATTATGTTGGT+TGG 0.435785 4.4:-75904046 MS.gene45853:CDS
ATTCTTTGGTATAGCATTCA+CGG 0.447221 4.4:+75904132 None:intergenic
ACTAACAGTTGTGTACTTCC+CGG 0.449602 4.4:+75904159 None:intergenic
CATTTCTATTCACAATTCTA+GGG 0.450955 4.4:-75902799 MS.gene45853:CDS
TTTGCAGCTGTTGTTGGAGA+TGG 0.451928 4.4:-75903128 MS.gene45853:intron
CCATGAACGAAGTTAAACTT+TGG 0.454650 4.4:-75904726 MS.gene45853:CDS
CACCTGGCCTAACCCATGTT+GGG 0.479286 4.4:+75904242 None:intergenic
AATATTATGTTGGTTGGAGA+TGG 0.480645 4.4:-75904040 MS.gene45853:CDS
ATGAGTTTGTGTTGGTAAGA+AGG 0.482647 4.4:+75904652 None:intergenic
AAAGGCAAAGAATATTATGT+TGG 0.483951 4.4:-75904050 MS.gene45853:CDS
CCATTTGACACGGTTTCTTG+TGG 0.485738 4.4:+75902572 None:intergenic
GAGTATATGAACACTGGTCT+TGG 0.488203 4.4:-75902600 MS.gene45853:CDS
TTGTGTTGACTTTGTTTGGA+GGG 0.490707 4.4:+75902862 None:intergenic
CAAGAACCTAGCTACAAAGA+TGG 0.493394 4.4:-75904268 MS.gene45853:CDS
TAGTAGCAAAAGACGGGTCC+GGG 0.495277 4.4:-75904177 MS.gene45853:CDS
ATTTCATTGCTGGAAATGCT+TGG 0.496886 4.4:-75902494 MS.gene45853:CDS
TGTAGTAGCTTTCTATCACC+TGG 0.499249 4.4:+75904226 None:intergenic
GTAGTAGCAAAAGACGGGTC+CGG 0.499299 4.4:-75904178 MS.gene45853:CDS
CCACAAGAAACCGTGTCAAA+TGG 0.501895 4.4:-75902572 MS.gene45853:CDS
AAGAAATACACACAAGGTTG+AGG 0.502901 4.4:+75904685 None:intergenic
GCCCTAGAATATTGCTTCCA+TGG 0.507314 4.4:+75902717 None:intergenic
TAAATTGCTAAGTAACACTT+TGG 0.507939 4.4:-75904311 MS.gene45853:CDS
GATAGTTTGATTCATCCAGC+TGG 0.516549 4.4:-75902672 MS.gene45853:CDS
TCACCTGGCCTAACCCATGT+TGG 0.518597 4.4:+75904241 None:intergenic
TTTGTTTGATGAGTTTGTGT+TGG 0.521126 4.4:+75904644 None:intergenic
AGTAGATGTAAATGGCACTT+TGG 0.524094 4.4:+75902461 None:intergenic
ACCGGTAGCAAAAGCGTTGG+AGG 0.524747 4.4:-75903998 MS.gene45853:CDS
AAATACAGACAAGTCTGATC+CGG 0.525381 4.4:+75903034 None:intergenic
ATGTTGGTTGGAGATGGTAT+TGG 0.525732 4.4:-75904034 MS.gene45853:CDS
AAAGCTGGATTCTATGATCT+TGG 0.527056 4.4:-75904376 MS.gene45853:CDS
TTCCCAACATGGGTTAGGCC+AGG 0.531406 4.4:-75904244 MS.gene45853:CDS
ATGGGTTCCCAACATGGGTT+AGG 0.533950 4.4:-75904249 MS.gene45853:CDS
TGAGTTTGTGTTGGTAAGAA+GGG 0.534506 4.4:+75904653 None:intergenic
ACAAAGATGGGTTCCCAACA+TGG 0.535204 4.4:-75904255 MS.gene45853:CDS
AGCTTTACATGTCTCAAGGT+TGG 0.546562 4.4:+75904393 None:intergenic
ACAATGCAACATTTATGGTA+CGG 0.547191 4.4:-75902946 MS.gene45853:CDS
CTCCATTCTAACCAACCAGC+TGG 0.548616 4.4:+75902657 None:intergenic
AACCTTGAGACATGTAAAGC+TGG 0.551306 4.4:-75904391 MS.gene45853:CDS
AAGAACCTAGCTACAAAGAT+GGG 0.557927 4.4:-75904267 MS.gene45853:CDS
TAGGTATGTTATACATGTGA+AGG 0.561706 4.4:-75904101 MS.gene45853:CDS
CTCAAGTTGATGCTCAAACA+TGG 0.563888 4.4:-75904432 MS.gene45853:CDS
ATCCAGCTTTACATGTCTCA+AGG 0.564983 4.4:+75904389 None:intergenic
ATTGTGTTGACTTTGTTTGG+AGG 0.571190 4.4:+75902861 None:intergenic
ACATTCCGTTCTGCCACTGT+TGG 0.587276 4.4:-75903965 MS.gene45853:intron
GATAACCTTGCCATTTGACA+CGG 0.589011 4.4:+75902562 None:intergenic
ACGTAGTAGTAGCAAAAGAC+GGG 0.589841 4.4:-75904183 MS.gene45853:CDS
AACGTAGTAGTAGCAAAAGA+CGG 0.590566 4.4:-75904184 MS.gene45853:CDS
GTTTGATTCATCCAGCTGGT+TGG 0.591836 4.4:-75902668 MS.gene45853:CDS
ACCATGGAAGCAATATTCTA+GGG 0.592357 4.4:-75902718 MS.gene45853:CDS
ATTGGTAAAACTGTAATCAC+CGG 0.592601 4.4:-75904016 MS.gene45853:CDS
CTAATGAAAGAGTGTTTGCA+TGG 0.596121 4.4:+75904566 None:intergenic
AGACTTATCTTGGAAGACCA+TGG 0.599466 4.4:-75902734 MS.gene45853:CDS
ATCACCGGTAGCAAAAGCGT+TGG 0.601310 4.4:-75904001 MS.gene45853:CDS
AGAAACCGTGTCAAATGGCA+AGG 0.607462 4.4:-75902567 MS.gene45853:CDS
TACCAAAGAATAACAAAGGT+AGG 0.608433 4.4:-75904120 MS.gene45853:CDS
GGTAGCAAAAGCGTTGGAGG+AGG 0.610712 4.4:-75903995 MS.gene45853:CDS
ATCACATTTAGGAACACTGC+CGG 0.612058 4.4:-75903089 MS.gene45853:CDS
GTAACATTGTTGGACATGAG+AGG 0.612493 4.4:+75904334 None:intergenic
CAAAGATGGGTTCCCAACAT+GGG 0.625227 4.4:-75904254 MS.gene45853:CDS
ACAGATCCTAACCAAAACAC+TGG 0.626471 4.4:-75902822 MS.gene45853:CDS
GCTATACCAAAGAATAACAA+AGG 0.633072 4.4:-75904124 MS.gene45853:CDS
TATGGAGAGTATATGAACAC+TGG 0.633586 4.4:-75902606 MS.gene45853:CDS
TATGTTATACATGTGAAGGC+TGG 0.634350 4.4:-75904097 MS.gene45853:CDS
ACTTACCAACAGTGGCAGAA+CGG 0.640460 4.4:+75903960 None:intergenic
GTTCAAGAAAGAGCACTCAA+AGG 0.644742 4.4:-75904589 MS.gene45853:CDS
GTCAACACAATCACTGCACA+AGG 0.651414 4.4:-75902849 MS.gene45853:CDS
CGGTTTCAAATCCGAAGCAG+CGG 0.662915 4.4:+75902773 None:intergenic
GCTGGTTGGTTAGAATGGAG+TGG 0.670039 4.4:-75902654 MS.gene45853:CDS
CAGTAACTACTTACCAACAG+TGG 0.681222 4.4:+75903952 None:intergenic
AGTGGCAGAACGGAATGTTG+TGG 0.699012 4.4:+75903970 None:intergenic
TTGTTCAAGAAATACACACA+AGG 0.713213 4.4:+75904679 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTAACATTATTTTTGTTT+TGG + chr4.4:75903470-75903489 None:intergenic 10.0%
!! CAATTTATTTAAATTGTTGA+AGG - chr4.4:75903848-75903867 MS.gene45853:intron 15.0%
!! TATTAATGTTAAAAACTAAG+AGG + chr4.4:75903985-75904004 None:intergenic 15.0%
!!! CTTGCAATTTTTTTTTATAT+AGG + chr4.4:75903684-75903703 None:intergenic 15.0%
!! GCATTAAGTACTTTATATTA+TGG - chr4.4:75904555-75904574 MS.gene45853:CDS 20.0%
!!! CTTTTATGTATTTATAGAAC+CGG - chr4.4:75903631-75903650 MS.gene45853:intron 20.0%
!!! TCTTGCAATTTTTTTGATAT+AGG + chr4.4:75903723-75903742 None:intergenic 20.0%
!!! TGAAATGTTGCTTATTATTA+AGG - chr4.4:75903291-75903310 MS.gene45853:intron 20.0%
!!! TTTGTTCCATTTTTTTAATG+CGG - chr4.4:75903792-75903811 MS.gene45853:intron 20.0%
!!! TTTTATAGATGTAGTTTTGA+AGG - chr4.4:75904165-75904184 MS.gene45853:CDS 20.0%
! AAAGGCAAAGAATATTATGT+TGG - chr4.4:75903129-75903148 MS.gene45853:intron 25.0%
! AATTCTGTTAAGACTTATCT+TGG - chr4.4:75904435-75904454 MS.gene45853:CDS 25.0%
! ATTATTAAGGTTGCCAAAAT+CGG - chr4.4:75903304-75903323 MS.gene45853:intron 25.0%
! CATTTCTATTCACAATTCTA+GGG - chr4.4:75904380-75904399 MS.gene45853:CDS 25.0%
! GTAAAACCGCATTAAAAAAA+TGG + chr4.4:75903801-75903820 None:intergenic 25.0%
! TAAATTGCTAAGTAACACTT+TGG - chr4.4:75902868-75902887 MS.gene45853:CDS 25.0%
! TACAAACAATGCAACATTTA+TGG - chr4.4:75904228-75904247 MS.gene45853:CDS 25.0%
! TAGCAATTTAGTAACATTGT+TGG + chr4.4:75902858-75902877 None:intergenic 25.0%
! TGATTTCTTCAAACTTTCAT+TGG - chr4.4:75902562-75902581 MS.gene45853:CDS 25.0%
!! GATAAGTCTTAACAGAATTT+TGG + chr4.4:75904434-75904453 None:intergenic 25.0%
!! TTGCAAATATGTTGCAATTT+TGG + chr4.4:75904293-75904312 None:intergenic 25.0%
!!! AACACTTTGTTTTAGTATCT+AGG + chr4.4:75902727-75902746 None:intergenic 25.0%
!!! ACACTTTGTTTTAGTATCTA+GGG + chr4.4:75902726-75902745 None:intergenic 25.0%
!!! CTGAATTTTTGGATTTAAGT+CGG - chr4.4:75903890-75903909 MS.gene45853:intron 25.0%
!!! TCGATTTTGATTACGATTTT+CGG + chr4.4:75903438-75903457 None:intergenic 25.0%
!!! TGAATTTTTGGATTTAAGTC+GGG - chr4.4:75903891-75903910 MS.gene45853:intron 25.0%
!!! TTTTTTAATGCGGTTTTACT+AGG - chr4.4:75903802-75903821 MS.gene45853:intron 25.0%
AAATTGCAGAATTCCAAAAC+AGG + chr4.4:75903834-75903853 None:intergenic 30.0%
ACAATGCAACATTTATGGTA+CGG - chr4.4:75904233-75904252 MS.gene45853:CDS 30.0%
ACTGTATCAAATTTCATTGC+TGG - chr4.4:75904675-75904694 MS.gene45853:CDS 30.0%
ATTCTTTGGTATAGCATTCA+CGG + chr4.4:75903050-75903069 None:intergenic 30.0%
ATTGGTAAAACTGTAATCAC+CGG - chr4.4:75903163-75903182 MS.gene45853:intron 30.0%
CGACTTAAATCCAAAAATTC+AGG + chr4.4:75903892-75903911 None:intergenic 30.0%
GCAAAGAATATTATGTTGGT+TGG - chr4.4:75903133-75903152 MS.gene45853:intron 30.0%
GCATTTCTATTCACAATTCT+AGG - chr4.4:75904379-75904398 MS.gene45853:CDS 30.0%
GCTATACCAAAGAATAACAA+AGG - chr4.4:75903055-75903074 MS.gene45853:CDS 30.0%
TACCAAAGAATAACAAAGGT+AGG - chr4.4:75903059-75903078 MS.gene45853:CDS 30.0%
TAGGTATGTTATACATGTGA+AGG - chr4.4:75903078-75903097 MS.gene45853:CDS 30.0%
TTATTAAGGTTGCCAAAATC+GGG - chr4.4:75903305-75903324 MS.gene45853:intron 30.0%
TTGTTCAAGAAATACACACA+AGG + chr4.4:75902503-75902522 None:intergenic 30.0%
TTTGTTTGATGAGTTTGTGT+TGG + chr4.4:75902538-75902557 None:intergenic 30.0%
! AATATTATGTTGGTTGGAGA+TGG - chr4.4:75903139-75903158 MS.gene45853:intron 30.0%
! CAATGAACAAGTTGAGATAA+AGG - chr4.4:75903111-75903130 MS.gene45853:CDS 30.0%
! TAAAATCCTGTGATTTTGCA+TGG - chr4.4:75903759-75903778 MS.gene45853:intron 30.0%
!! GGTACGGTTGATTTTATATT+TGG - chr4.4:75904249-75904268 MS.gene45853:CDS 30.0%
!! TACCTACCTTTGTTATTCTT+TGG + chr4.4:75903064-75903083 None:intergenic 30.0%
AAAGCTGGATTCTATGATCT+TGG - chr4.4:75902803-75902822 MS.gene45853:CDS 35.0%
AAATACAGACAAGTCTGATC+CGG + chr4.4:75904148-75904167 None:intergenic 35.0%
AACGTAGTAGTAGCAAAAGA+CGG - chr4.4:75902995-75903014 MS.gene45853:CDS 35.0%
AAGAAATACACACAAGGTTG+AGG + chr4.4:75902497-75902516 None:intergenic 35.0%
AAGAACCTAGCTACAAAGAT+GGG - chr4.4:75902912-75902931 MS.gene45853:CDS 35.0%
ACCATGGAAGCAATATTCTA+GGG - chr4.4:75904461-75904480 MS.gene45853:CDS 35.0%
AGTAGATGTAAATGGCACTT+TGG + chr4.4:75904721-75904740 None:intergenic 35.0%
ATCTAAGCTTCGAGTCATTT+AGG + chr4.4:75903556-75903575 None:intergenic 35.0%
ATTTCATTGCTGGAAATGCT+TGG - chr4.4:75904685-75904704 MS.gene45853:CDS 35.0%
CATGCAAACACTCTTTCATT+AGG - chr4.4:75902614-75902633 MS.gene45853:CDS 35.0%
GTATTTATAGAACCGGATCA+CGG - chr4.4:75903638-75903657 MS.gene45853:intron 35.0%
TAAAAGACCAAGTTCTGTCT+AGG + chr4.4:75903617-75903636 None:intergenic 35.0%
TAAACAACCTAGACAGAACT+TGG - chr4.4:75903607-75903626 MS.gene45853:intron 35.0%
TATGTTATACATGTGAAGGC+TGG - chr4.4:75903082-75903101 MS.gene45853:CDS 35.0%
TATTTATAGAACCGGATCAC+GGG - chr4.4:75903639-75903658 MS.gene45853:intron 35.0%
TGAAATCCATGCAAAATCAC+AGG + chr4.4:75903768-75903787 None:intergenic 35.0%
TGGCTCAAGATATCACATTT+AGG - chr4.4:75904079-75904098 MS.gene45853:CDS 35.0%
TTCACGTGAGTTACTACATT+TGG + chr4.4:75902642-75902661 None:intergenic 35.0%
TTTATATAGGTCAATCGACC+CGG + chr4.4:75903671-75903690 None:intergenic 35.0%
! ATGAGTTTGTGTTGGTAAGA+AGG + chr4.4:75902530-75902549 None:intergenic 35.0%
! CAAAATCGGGATTTAACTTC+AGG - chr4.4:75903318-75903337 MS.gene45853:intron 35.0%
! CTGAAGTTAAATCCCGATTT+TGG + chr4.4:75903320-75903339 None:intergenic 35.0%
! GAATAGAAATGCCAGTGTTT+TGG + chr4.4:75904371-75904390 None:intergenic 35.0%
! GATTTAACTTCAGGTCGAAT+GGG - chr4.4:75903327-75903346 MS.gene45853:intron 35.0%
! GTCGCAAAATCCTGAATTTT+TGG - chr4.4:75903879-75903898 MS.gene45853:intron 35.0%
! GTCTTAACAGAATTTTGGAC+CGG + chr4.4:75904429-75904448 None:intergenic 35.0%
! TATGGAGAGTATATGAACAC+TGG - chr4.4:75904573-75904592 MS.gene45853:CDS 35.0%
! TCTTTTGCTACTACTACGTT+AGG + chr4.4:75902995-75903014 None:intergenic 35.0%
! TGAGTTTGTGTTGGTAAGAA+GGG + chr4.4:75902529-75902548 None:intergenic 35.0%
! TGTTGTTGGAGATGGATTTA+TGG - chr4.4:75904059-75904078 MS.gene45853:CDS 35.0%
!! ATTGTGTTGACTTTGTTTGG+AGG + chr4.4:75904321-75904340 None:intergenic 35.0%
!! CTAATGAAAGAGTGTTTGCA+TGG + chr4.4:75902616-75902635 None:intergenic 35.0%
!! GTGATTGTGTTGACTTTGTT+TGG + chr4.4:75904324-75904343 None:intergenic 35.0%
!! TTGTGTTGACTTTGTTTGGA+GGG + chr4.4:75904320-75904339 None:intergenic 35.0%
AACCTTGAGACATGTAAAGC+TGG - chr4.4:75902788-75902807 MS.gene45853:CDS 40.0%
AAGGTTGAGGATTAGGTGTT+TGG + chr4.4:75902484-75902503 None:intergenic 40.0%
ACAGATCCTAACCAAAACAC+TGG - chr4.4:75904357-75904376 MS.gene45853:CDS 40.0%
ACGTAGTAGTAGCAAAAGAC+GGG - chr4.4:75902996-75903015 MS.gene45853:CDS 40.0%
ACTAACAGTTGTGTACTTCC+CGG + chr4.4:75903023-75903042 None:intergenic 40.0%
ACTCACGTGAAAAATCAGCT+TGG - chr4.4:75902651-75902670 MS.gene45853:CDS 40.0%
AGACTTATCTTGGAAGACCA+TGG - chr4.4:75904445-75904464 MS.gene45853:CDS 40.0%
AGCTTTACATGTCTCAAGGT+TGG + chr4.4:75902789-75902808 None:intergenic 40.0%
ATCACATTTAGGAACACTGC+CGG - chr4.4:75904090-75904109 MS.gene45853:CDS 40.0%
ATCCAGCTTTACATGTCTCA+AGG + chr4.4:75902793-75902812 None:intergenic 40.0%
CAAGAACCTAGCTACAAAGA+TGG - chr4.4:75902911-75902930 MS.gene45853:CDS 40.0%
CAGTAACTACTTACCAACAG+TGG + chr4.4:75903230-75903249 None:intergenic 40.0%
CTCACGTGAAAAATCAGCTT+GGG - chr4.4:75902652-75902671 MS.gene45853:CDS 40.0%
GACCATGGAAGCAATATTCT+AGG - chr4.4:75904460-75904479 MS.gene45853:CDS 40.0%
GATAGTTTGATTCATCCAGC+TGG - chr4.4:75904507-75904526 MS.gene45853:CDS 40.0%
GTAACATTGTTGGACATGAG+AGG + chr4.4:75902848-75902867 None:intergenic 40.0%
TACACACAAGGTTGAGGATT+AGG + chr4.4:75902491-75902510 None:intergenic 40.0%
TGTAGTAGCTTTCTATCACC+TGG + chr4.4:75902956-75902975 None:intergenic 40.0%
! ATGTTGGTTGGAGATGGTAT+TGG - chr4.4:75903145-75903164 MS.gene45853:intron 40.0%
! GAGTATATGAACACTGGTCT+TGG - chr4.4:75904579-75904598 MS.gene45853:CDS 40.0%
! GATAACCTTGCCATTTGACA+CGG + chr4.4:75904620-75904639 None:intergenic 40.0%
! GGATTTAACTTCAGGTCGAA+TGG - chr4.4:75903326-75903345 MS.gene45853:intron 40.0%
! TACTAGGATCTTGCCTGTTT+TGG - chr4.4:75903818-75903837 MS.gene45853:intron 40.0%
! TGTCTTTTTGCAGCTGTTGT+TGG - chr4.4:75904045-75904064 MS.gene45853:CDS 40.0%
!! CTCAAGTTGATGCTCAAACA+TGG - chr4.4:75902747-75902766 MS.gene45853:CDS 40.0%
!! GTTCAAGAAAGAGCACTCAA+AGG - chr4.4:75902590-75902609 MS.gene45853:CDS 40.0%
!!! AGCTTGATGGTTTTTTGCAC+CGG + chr4.4:75904112-75904131 None:intergenic 40.0%
!!! CTAGGGTTTTGTTGAGCTTA+AGG + chr4.4:75902709-75902728 None:intergenic 40.0%
!!! GAAATGCCAGTGTTTTGGTT+AGG + chr4.4:75904366-75904385 None:intergenic 40.0%
!!! GGTTTTGTTGAGCTTAAGGA+TGG + chr4.4:75902705-75902724 None:intergenic 40.0%
AACGCAATGCAACAGCTTGA+TGG + chr4.4:75904125-75904144 None:intergenic 45.0%
ACTTACCAACAGTGGCAGAA+CGG + chr4.4:75903222-75903241 None:intergenic 45.0%
AGAAACCGTGTCAAATGGCA+AGG - chr4.4:75904612-75904631 MS.gene45853:CDS 45.0%
CCACAAGAAACCGTGTCAAA+TGG - chr4.4:75904607-75904626 MS.gene45853:CDS 45.0%
CCATTTGACACGGTTTCTTG+TGG + chr4.4:75904610-75904629 None:intergenic 45.0%
GCCCTAGAATATTGCTTCCA+TGG + chr4.4:75904465-75904484 None:intergenic 45.0%
GTAAATGGCACTTTGGTTGC+AGG + chr4.4:75904714-75904733 None:intergenic 45.0%
GTCAACACAATCACTGCACA+AGG - chr4.4:75904330-75904349 MS.gene45853:CDS 45.0%
GTTTGATTCATCCAGCTGGT+TGG - chr4.4:75904511-75904530 MS.gene45853:CDS 45.0%
TTTGCAGCTGTTGTTGGAGA+TGG - chr4.4:75904051-75904070 MS.gene45853:CDS 45.0%
! ACAAAGATGGGTTCCCAACA+TGG - chr4.4:75902924-75902943 MS.gene45853:CDS 45.0%
! ATCCAGCTGGTTGGTTAGAA+TGG - chr4.4:75904520-75904539 MS.gene45853:CDS 45.0%
! CAAAGATGGGTTCCCAACAT+GGG - chr4.4:75902925-75902944 MS.gene45853:CDS 45.0%
ACATTCCGTTCTGCCACTGT+TGG - chr4.4:75903214-75903233 MS.gene45853:intron 50.0%
ACCGGATCACGGGTATAATC+CGG - chr4.4:75903649-75903668 MS.gene45853:intron 50.0%
AGTGGCAGAACGGAATGTTG+TGG + chr4.4:75903212-75903231 None:intergenic 50.0%
ATGGGTTCCCAACATGGGTT+AGG - chr4.4:75902930-75902949 MS.gene45853:CDS 50.0%
CAAGCTGTTGCATTGCGTTC+CGG - chr4.4:75904126-75904145 MS.gene45853:CDS 50.0%
CGGTTTCAAATCCGAAGCAG+CGG + chr4.4:75904409-75904428 None:intergenic 50.0%
CTCCATTCTAACCAACCAGC+TGG + chr4.4:75904525-75904544 None:intergenic 50.0%
GGGAACCCATCTTTGTAGCT+AGG + chr4.4:75902920-75902939 None:intergenic 50.0%
GTAGTAGCAAAAGACGGGTC+CGG - chr4.4:75903001-75903020 MS.gene45853:CDS 50.0%
TAGTAGCAAAAGACGGGTCC+GGG - chr4.4:75903002-75903021 MS.gene45853:CDS 50.0%
! ATCACCGGTAGCAAAAGCGT+TGG - chr4.4:75903178-75903197 MS.gene45853:intron 50.0%
! CGCTGCTTCGGATTTGAAAC+CGG - chr4.4:75904407-75904426 MS.gene45853:CDS 50.0%
! GCTGGTTGGTTAGAATGGAG+TGG - chr4.4:75904525-75904544 MS.gene45853:CDS 50.0%
! TCCTCCAACGCTTTTGCTAC+CGG + chr4.4:75903185-75903204 None:intergenic 50.0%
!! TTCTAGGGTTACCGCTGCTT+CGG - chr4.4:75904395-75904414 MS.gene45853:CDS 50.0%
CACCTGGCCTAACCCATGTT+GGG + chr4.4:75902940-75902959 None:intergenic 55.0%
CCCGGATTATACCCGTGATC+CGG + chr4.4:75903653-75903672 None:intergenic 55.0%
CCGGATCACGGGTATAATCC+GGG - chr4.4:75903650-75903669 MS.gene45853:intron 55.0%
TCACCTGGCCTAACCCATGT+TGG + chr4.4:75902941-75902960 None:intergenic 55.0%
!! ACCGGTAGCAAAAGCGTTGG+AGG - chr4.4:75903181-75903200 MS.gene45853:intron 55.0%
!! GGTAGCAAAAGCGTTGGAGG+AGG - chr4.4:75903184-75903203 MS.gene45853:intron 55.0%
!! TTCCCAACATGGGTTAGGCC+AGG - chr4.4:75902935-75902954 MS.gene45853:CDS 55.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 75902455 75904746 75902455 ID=MS.gene45853
chr4.4 mRNA 75902455 75904746 75902455 ID=MS.gene45853.t1;Parent=MS.gene45853
chr4.4 exon 75903966 75904746 75903966 ID=MS.gene45853.t1.exon1;Parent=MS.gene45853.t1
chr4.4 CDS 75903966 75904746 75903966 ID=cds.MS.gene45853.t1;Parent=MS.gene45853.t1
chr4.4 exon 75902455 75903143 75902455 ID=MS.gene45853.t1.exon2;Parent=MS.gene45853.t1
chr4.4 CDS 75902455 75903143 75902455 ID=cds.MS.gene45853.t1;Parent=MS.gene45853.t1
Gene Sequence

>MS.gene45853

ATGAACGAAGTTAAACTTTGGTGTAACCAAACACCTAATCCTCAACCTTGTGTGTATTTCTTGAACAATAACCCTTCTTACCAACACAAACTCATCAAACAAAAAGTTGATTTCTTCAAACTTTCATTGGAACTTGTTCAAGAAAGAGCACTCAAAGGCCATGCAAACACTCTTTCATTAGGCTCCAAATGTAGTAACTCACGTGAAAAATCAGCTTGGGCTGATTGTGTTGAGCTATATGAACAAACCATCCTTAAGCTCAACAAAACCCTAGATACTAAAACAAAGTGTTCTCAAGTTGATGCTCAAACATGGCTAAGCACAGCTCTCACCAACCTTGAGACATGTAAAGCTGGATTCTATGATCTTGGTGTTCAAGATTATATTCTTCCTCTCATGTCCAACAATGTTACTAAATTGCTAAGTAACACTTTGGCTCTTAACAAAGTTCAATATCAAGAACCTAGCTACAAAGATGGGTTCCCAACATGGGTTAGGCCAGGTGATAGAAAGCTACTACAAACTTCGTCTTCTCGACCTAACGTAGTAGTAGCAAAAGACGGGTCCGGGAAGTACACAACTGTTAGTGCAGCCGTGAATGCTATACCAAAGAATAACAAAGGTAGGTATGTTATACATGTGAAGGCTGGTGTCTACAATGAACAAGTTGAGATAAAGGCAAAGAATATTATGTTGGTTGGAGATGGTATTGGTAAAACTGTAATCACCGGTAGCAAAAGCGTTGGAGGAGGAACCACAACATTCCGTTCTGCCACTGTTGGTAAGTAGTTACTGCTAAAAAAAATTATTTATTGATACAAGTTTGAGATACAATATGAAATGTTGCTTATTATTAAGGTTGCCAAAATCGGGATTTAACTTCAGGTCGAATGGGACTAGCAAAATCAAAAATCATAAAATTGCAATCAAAATCGAAAAATTTTACTCAACTACTTTTGTATAATCGATATATAGAAAAACCGAAAATCGTAATCAAAATCGATAGATTTTACCAAAACAAAAATAATGTTAAATATATGCTATGAATTTTCTAGAGTCATAAATGACTCAAAGTTTAAAGTCTACAAAATACTTATACCTAAATGACTCGAAGCTTAGATGTCAAATATTCTTTAGTAGTCATAATAATACTAAACAACCTAGACAGAACTTGGTCTTTTATGTATTTATAGAACCGGATCACGGGTATAATCCGGGTCGATTGACCTATATAAAAAAAAATTGCAAGAATCATTCAATATTGACCTATATCAAAAAAATTGCAAGAATCATTCAAAATCTTGTAAAATCCTGTGATTTTGCATGGATTTCATGATTTTGTTCCATTTTTTTAATGCGGTTTTACTAGGATCTTGCCTGTTTTGGAATTCTGCAATTTATTTAAATTGTTGAAGGTGAATAAAGTCGCAAAATCCTGAATTTTTGGATTTAAGTCGGGATTTGACAACAATGCTTATTATGTCTTTAATTGTTATACATGTGTGTGTATCTAGATTAAAACATTCTCCTCTTAGTTTTTAACATTAATAGAATACACTCAATGACTAGAAATCTAACGAATATATATTATGTCTTTTTGCAGCTGTTGTTGGAGATGGATTTATGGCTCAAGATATCACATTTAGGAACACTGCCGGTGCAAAAAACCATCAAGCTGTTGCATTGCGTTCCGGATCAGACTTGTCTGTATTTTATAGATGTAGTTTTGAAGGTTTTCAAGACACATTATATGTGCATTCAGATAGACAATTCTACAAACAATGCAACATTTATGGTACGGTTGATTTTATATTTGGTAATGCTGCTGCAGTGTTCCAAAATTGCAACATATTTGCAAGAAACCCTCCAAACAAAGTCAACACAATCACTGCACAAGGTAGAACAGATCCTAACCAAAACACTGGCATTTCTATTCACAATTCTAGGGTTACCGCTGCTTCGGATTTGAAACCGGTCCAAAATTCTGTTAAGACTTATCTTGGAAGACCATGGAAGCAATATTCTAGGGCAGTTTTTATGAAGACTTCTCTTGATAGTTTGATTCATCCAGCTGGTTGGTTAGAATGGAGTGGTAATTTTGCATTAAGTACTTTATATTATGGAGAGTATATGAACACTGGTCTTGGTTCTTCCACAAGAAACCGTGTCAAATGGCAAGGTTATCATGTGATAACAAGTGCTTCTGAAGCATCAAAGTTCACTGTATCAAATTTCATTGCTGGAAATGCTTGGTTACCTGCAACCAAAGTGCCATTTACATCTACTCTTTAA
Protein sequence

>MS.gene45853.t1

MNEVKLWCNQTPNPQPCVYFLNNNPSYQHKLIKQKVDFFKLSLELVQERALKGHANTLSLGSKCSNSREKSAWADCVELYEQTILKLNKTLDTKTKCSQVDAQTWLSTALTNLETCKAGFYDLGVQDYILPLMSNNVTKLLSNTLALNKVQYQEPSYKDGFPTWVRPGDRKLLQTSSSRPNVVVAKDGSGKYTTVSAAVNAIPKNNKGRYVIHVKAGVYNEQVEIKAKNIMLVGDGIGKTVITGSKSVGGGTTTFRSATVAVVGDGFMAQDITFRNTAGAKNHQAVALRSGSDLSVFYRCSFEGFQDTLYVHSDRQFYKQCNIYGTVDFIFGNAAAVFQNCNIFARNPPNKVNTITAQGRTDPNQNTGISIHNSRVTAASDLKPVQNSVKTYLGRPWKQYSRAVFMKTSLDSLIHPAGWLEWSGNFALSTLYYGEYMNTGLGSSTRNRVKWQGYHVITSASEASKFTVSNFIAGNAWLPATKVPFTSTL