AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene48246

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene48246.t1 MTR_4g025690 97.912 479 10 0 1 479 25 503 0.0 981
MS.gene48246.t1 MTR_4g025730 74.486 486 114 6 1 483 26 504 0.0 750
MS.gene48246.t1 MTR_8g104655 50.688 509 216 11 5 483 33 536 3.97e-175 508
MS.gene48246.t1 MTR_3g033690 53.768 491 208 10 4 480 32 517 1.19e-173 504
MS.gene48246.t1 MTR_4g035980 51.000 500 224 10 4 483 36 534 1.02e-171 500
MS.gene48246.t1 MTR_3g008640 53.673 490 207 11 4 478 32 516 1.64e-171 499
MS.gene48246.t1 MTR_3g008720 53.673 490 207 11 4 478 32 516 3.04e-171 498
MS.gene48246.t1 MTR_7g053360 53.673 490 207 11 4 478 32 516 1.13e-170 497
MS.gene48246.t1 MTR_3g008700 53.049 492 211 11 2 478 30 516 9.60e-170 494
MS.gene48246.t1 MTR_7g108905 48.515 505 229 12 8 483 49 551 1.53e-155 459
MS.gene48246.t1 MTR_4g025670 53.286 426 179 9 72 483 65 484 1.58e-150 444
MS.gene48246.t1 MTR_8g104645 62.687 335 115 5 159 483 33 367 2.09e-150 439
MS.gene48246.t1 MTR_4g087980 51.501 433 185 6 66 479 80 506 5.42e-148 438
MS.gene48246.t1 MTR_8g104580 45.079 508 213 12 5 483 25 495 2.36e-146 434
MS.gene48246.t1 MTR_8g023310 46.360 522 239 11 4 486 68 587 9.66e-145 432
MS.gene48246.t1 MTR_7g050980 48.104 501 222 13 6 478 54 544 4.82e-143 427
MS.gene48246.t1 MTR_2g044880 50.343 437 197 5 61 479 81 515 8.04e-143 426
MS.gene48246.t1 MTR_1g008140 43.411 516 244 10 8 479 86 597 5.11e-142 426
MS.gene48246.t1 MTR_7g050870 52.294 436 184 9 63 478 112 543 2.28e-141 422
MS.gene48246.t1 MTR_1g083520 48.718 507 214 14 8 483 32 523 2.61e-141 422
MS.gene48246.t1 MTR_3g091380 44.055 513 239 10 8 479 82 587 2.69e-140 421
MS.gene48246.t1 MTR_1g088020 47.259 529 216 18 8 483 32 550 2.13e-136 410
MS.gene48246.t1 MTR_7g108890 46.360 522 223 14 8 480 40 553 3.06e-135 407
MS.gene48246.t1 MTR_7g112580 48.770 447 184 10 66 480 100 533 8.78e-134 403
MS.gene48246.t1 MTR_1g088225 47.070 529 215 19 8 483 32 548 2.58e-133 402
MS.gene48246.t1 MTR_7g050950 50.461 434 194 9 63 478 112 542 3.64e-133 402
MS.gene48246.t1 MTR_1g054880 49.565 460 182 12 72 483 96 553 2.68e-132 399
MS.gene48246.t1 MTR_7g108900 46.241 532 227 17 8 483 27 555 3.19e-132 399
MS.gene48246.t1 MTR_4g087970 46.682 437 202 6 67 479 78 507 1.09e-129 391
MS.gene48246.t1 MTR_8g042900 44.752 505 241 14 4 479 76 571 1.81e-128 390
MS.gene48246.t1 MTR_7g108875 43.678 522 237 12 8 480 42 555 2.71e-127 387
MS.gene48246.t1 MTR_8g042910 47.521 484 191 13 51 479 127 602 2.63e-126 386
MS.gene48246.t1 MTR_3g010780 42.495 513 252 13 2 483 69 569 4.03e-122 374
MS.gene48246.t1 MTR_4g124550 42.054 516 261 11 4 486 81 591 1.52e-120 370
MS.gene48246.t1 MTR_2g044810 41.566 498 259 10 1 478 38 523 7.98e-120 367
MS.gene48246.t1 MTR_0458s0030 64.729 258 88 3 228 483 53 309 1.12e-117 353
MS.gene48246.t1 MTR_1g088040 64.729 258 88 3 228 483 53 309 1.12e-117 353
MS.gene48246.t1 MTR_3g010770 58.553 304 119 4 182 479 263 565 3.81e-117 361
MS.gene48246.t1 MTR_8g023150 42.292 506 252 12 4 479 77 572 3.59e-116 358
MS.gene48246.t1 MTR_4g124520 47.103 397 184 10 100 478 122 510 9.63e-116 355
MS.gene48246.t1 MTR_0618s0020 68.161 223 69 2 263 483 1 223 1.90e-111 334
MS.gene48246.t1 MTR_1g086190 39.673 489 254 12 37 486 75 561 1.86e-106 333
MS.gene48246.t1 MTR_1g086210 49.530 319 156 3 159 472 228 546 4.26e-106 332
MS.gene48246.t1 MTR_8g093460 39.181 513 263 14 2 478 41 540 3.45e-102 322
MS.gene48246.t1 MTR_3g095920 38.921 519 270 14 1 480 45 555 3.06e-100 317
MS.gene48246.t1 MTR_2g044660 48.338 331 164 4 159 486 304 630 3.26e-100 319
MS.gene48246.t1 MTR_7g110490 39.270 466 228 11 70 487 106 564 7.94e-98 311
MS.gene48246.t1 MTR_7g110510 39.270 466 228 11 70 487 106 564 7.94e-98 311
MS.gene48246.t1 MTR_7g110440 38.043 460 227 10 70 481 106 555 6.36e-97 308
MS.gene48246.t1 MTR_1g083510 37.121 528 268 16 4 478 56 572 8.61e-95 303
MS.gene48246.t1 MTR_1g083470 36.158 531 271 17 1 483 55 565 1.02e-94 303
MS.gene48246.t1 MTR_1g083500 36.158 531 271 17 1 483 55 565 1.02e-94 303
MS.gene48246.t1 MTR_7g110560 38.806 469 250 12 48 483 80 544 4.08e-94 300
MS.gene48246.t1 MTR_7g112590 36.694 496 259 12 32 478 83 572 6.95e-94 301
MS.gene48246.t1 MTR_4g005170 38.274 452 241 12 66 483 123 570 6.70e-92 297
MS.gene48246.t1 MTR_1g083490 37.832 452 243 12 66 483 123 570 1.01e-91 295
MS.gene48246.t1 MTR_2g436040 33.464 511 309 12 4 489 43 547 2.18e-90 292
MS.gene48246.t1 MTR_4g005180 37.310 461 233 13 66 483 105 552 4.39e-90 290
MS.gene48246.t1 MTR_4g120290 36.122 526 273 15 4 483 59 567 6.87e-90 290
MS.gene48246.t1 MTR_4g120280 34.667 525 282 13 4 483 59 567 1.53e-88 287
MS.gene48246.t1 MTR_2g044810 53.750 240 109 2 240 478 216 454 1.78e-87 280
MS.gene48246.t1 MTR_8g088610 43.939 330 175 8 161 483 45 371 4.71e-86 274
MS.gene48246.t1 MTR_2g436050 36.730 471 265 12 48 486 93 562 4.14e-85 277
MS.gene48246.t1 MTR_7g110420 35.246 488 250 12 70 501 106 583 4.87e-84 275
MS.gene48246.t1 MTR_5g083620 42.478 339 178 8 159 485 20 353 1.18e-83 267
MS.gene48246.t1 MTR_0458s0010 38.447 528 227 21 8 483 32 513 1.95e-80 263
MS.gene48246.t1 MTR_7g110430 33.333 459 216 10 70 481 106 521 4.56e-77 255
MS.gene48246.t1 MTR_2g105560 40.391 307 167 6 181 483 40 334 1.95e-69 229
MS.gene48246.t1 MTR_7g107180 40.645 310 170 6 178 483 3 302 4.56e-68 224
MS.gene48246.t1 MTR_6g024165 39.938 323 169 8 169 480 28 336 3.72e-66 220
MS.gene48246.t1 MTR_2g039530 42.434 304 158 6 158 456 28 319 1.22e-62 211
MS.gene48246.t1 MTR_7g111560 30.478 502 309 17 8 483 64 551 4.74e-60 210
MS.gene48246.t1 MTR_1g086540 36.646 322 178 6 169 478 37 344 3.78e-56 194
MS.gene48246.t1 MTR_8g102380 38.816 304 157 7 190 478 88 377 1.54e-54 191
MS.gene48246.t1 MTR_6g088810 39.474 304 153 7 190 478 90 377 6.98e-54 189
MS.gene48246.t1 MTR_6g012800 35.082 305 169 7 182 472 6 295 1.15e-50 178
MS.gene48246.t1 MTR_6g042660 36.111 288 156 7 171 451 30 296 5.10e-48 171
MS.gene48246.t1 MTR_8g102380 41.129 248 120 6 190 422 88 324 2.34e-46 167
MS.gene48246.t1 MTR_7g092780 32.132 333 195 7 159 478 73 387 4.79e-46 167
MS.gene48246.t1 MTR_7g011530 34.219 301 173 6 184 472 8 295 1.61e-45 164
MS.gene48246.t1 MTR_5g083590 32.890 301 148 5 182 478 7 257 1.10e-44 160
MS.gene48246.t1 MTR_5g095490 34.416 308 168 7 188 478 67 357 7.08e-44 161
MS.gene48246.t1 MTR_6g042690 35.211 284 151 8 182 451 39 303 1.25e-43 159
MS.gene48246.t1 MTR_4g124740 34.628 309 167 8 182 478 54 339 5.84e-43 158
MS.gene48246.t1 MTR_7g102600 32.787 305 183 6 184 478 101 393 2.59e-42 157
MS.gene48246.t1 MTR_1g033050 33.333 285 167 5 177 451 13 284 4.68e-42 154
MS.gene48246.t1 MTR_2g033970 32.188 320 193 6 172 482 46 350 1.29e-41 154
MS.gene48246.t1 MTR_5g012600 31.190 311 186 7 182 478 63 359 4.79e-39 147
MS.gene48246.t1 MTR_5g093970 33.443 305 177 9 182 478 43 329 5.26e-38 144
MS.gene48246.t1 MTR_5g088300 32.215 298 183 5 182 478 37 316 6.43e-38 143
MS.gene48246.t1 MTR_2g073390 30.189 318 200 7 169 478 58 361 2.19e-36 140
MS.gene48246.t1 MTR_2g436600 31.132 318 184 9 172 479 45 337 4.16e-36 139
MS.gene48246.t1 MTR_4g074290 30.841 321 194 8 169 478 58 361 7.07e-35 136
MS.gene48246.t1 MTR_5g088280 31.229 301 188 5 179 478 36 318 9.47e-35 134
MS.gene48246.t1 MTR_4g051810 32.441 299 182 6 182 478 37 317 1.00e-34 134
MS.gene48246.t1 MTR_5g088290 31.561 301 182 7 182 478 48 328 8.65e-34 132
MS.gene48246.t1 MTR_0009s0350 29.063 320 201 8 169 478 58 361 2.69e-31 125
MS.gene48246.t1 MTR_0009s0340 29.063 320 201 8 169 478 58 361 2.69e-31 125
MS.gene48246.t1 MTR_8g036225 27.564 312 198 6 182 483 33 326 1.04e-29 120
MS.gene48246.t1 MTR_6g025590 38.122 181 102 3 169 339 36 216 4.62e-29 116
MS.gene48246.t1 MTR_2g436070 28.315 279 156 10 4 251 56 321 1.13e-19 91.7
MS.gene48246.t1 MTR_0408s0030 41.414 99 45 3 355 451 36 123 3.86e-16 76.6
MS.gene48246.t1 MTR_4g130790 28.112 249 147 8 185 420 25 254 6.73e-16 78.6
MS.gene48246.t1 MTR_6g024200 34.375 160 84 5 320 478 137 276 2.63e-15 77.0
MS.gene48246.t1 MTR_7g102580 40.659 91 44 2 249 334 68 153 2.40e-12 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene48246.t1 AT2G45220 67.427 482 152 3 3 483 29 506 0.0 698
MS.gene48246.t1 AT5G51490 51.102 499 218 10 7 483 37 531 2.25e-172 501
MS.gene48246.t1 AT5G51500 50.704 497 223 10 7 483 41 535 1.00e-166 487
MS.gene48246.t1 AT3G47400 48.915 507 233 10 3 483 83 589 1.94e-165 486
MS.gene48246.t1 AT4G00190 53.459 477 197 9 8 479 8 464 3.09e-165 481
MS.gene48246.t1 AT1G23200 47.818 527 213 14 8 485 38 551 4.90e-150 445
MS.gene48246.t1 AT3G43270 49.045 471 208 8 39 479 47 515 7.32e-143 426
MS.gene48246.t1 AT3G49220 44.689 499 246 8 8 479 91 586 1.26e-140 422
MS.gene48246.t1 AT3G49220 44.689 499 246 8 8 479 136 631 7.16e-140 422
MS.gene48246.t1 AT3G14310 49.362 470 186 10 68 486 122 590 1.04e-139 420
MS.gene48246.t1 AT3G60730 47.505 461 199 8 62 483 58 514 1.45e-139 417
MS.gene48246.t1 AT5G53370 43.713 501 249 8 8 479 79 575 3.32e-139 418
MS.gene48246.t1 AT3G59010 49.460 463 202 8 39 479 66 518 4.93e-139 416
MS.gene48246.t1 AT5G53370 43.713 501 249 8 8 479 115 611 1.27e-138 418
MS.gene48246.t1 AT1G53830 45.369 529 240 12 4 487 62 586 2.43e-136 411
MS.gene48246.t1 AT1G11580 49.886 437 194 8 68 482 118 551 9.47e-136 408
MS.gene48246.t1 AT1G11580 49.886 437 194 8 68 482 139 572 1.43e-135 409
MS.gene48246.t1 AT4G02320 46.847 444 209 10 60 478 66 507 5.47e-133 400
MS.gene48246.t1 AT4G33220 46.035 454 203 6 66 479 62 513 7.93e-133 400
MS.gene48246.t1 AT4G33220 56.832 322 136 2 161 479 71 392 2.46e-130 389
MS.gene48246.t1 AT3G05620 49.213 445 182 11 67 479 99 531 1.47e-129 392
MS.gene48246.t1 AT4G02330 43.519 540 233 19 8 483 35 566 2.94e-125 382
MS.gene48246.t1 AT2G43050 48.926 419 188 10 75 479 100 506 3.32e-125 380
MS.gene48246.t1 AT4G02300 45.067 446 212 11 69 486 90 530 7.35e-123 374
MS.gene48246.t1 AT2G26450 42.095 506 265 11 4 486 109 609 5.70e-122 375
MS.gene48246.t1 AT1G02810 42.495 553 229 16 8 483 32 572 1.33e-121 373
MS.gene48246.t1 AT3G10720 42.105 532 241 15 8 480 83 606 1.55e-118 366
MS.gene48246.t1 AT2G47550 42.205 526 243 14 8 483 39 553 5.34e-118 363
MS.gene48246.t1 AT1G53840 40.698 516 255 12 4 479 73 577 7.06e-118 363
MS.gene48246.t1 AT5G04960 41.352 503 252 13 4 479 68 554 1.76e-117 362
MS.gene48246.t1 AT2G26440 40.472 509 254 12 7 478 40 536 9.83e-115 354
MS.gene48246.t1 AT5G20860 40.079 504 268 12 2 479 33 528 1.04e-114 353
MS.gene48246.t1 AT3G10710 41.948 503 244 13 4 479 70 551 3.57e-114 353
MS.gene48246.t1 AT3G05610 41.741 448 228 11 66 483 116 560 1.84e-113 354
MS.gene48246.t1 AT5G49180 41.909 482 242 14 34 479 81 560 2.22e-112 348
MS.gene48246.t1 AT5G04970 41.651 533 242 17 8 480 88 611 3.43e-112 350
MS.gene48246.t1 AT4G33230 43.363 452 219 11 60 478 148 595 1.85e-111 347
MS.gene48246.t1 AT3G06830 39.540 478 249 11 39 480 86 559 2.99e-111 345
MS.gene48246.t1 AT3G14300 39.845 517 257 13 8 483 463 966 7.48e-111 355
MS.gene48246.t1 AT5G27870 41.216 444 234 9 66 483 115 557 1.18e-110 349
MS.gene48246.t1 AT3G10720 61.355 251 95 2 231 480 1 250 5.59e-106 321
MS.gene48246.t1 AT4G15980 47.661 342 170 5 147 480 353 693 5.16e-102 325
MS.gene48246.t1 AT4G15980 47.661 342 170 5 147 480 363 703 1.08e-101 325
MS.gene48246.t1 AT3G27980 43.636 385 208 5 103 480 106 488 3.73e-101 317
MS.gene48246.t1 AT5G64640 39.181 513 267 17 3 477 92 597 3.38e-98 313
MS.gene48246.t1 AT1G11590 43.445 389 207 5 101 478 127 513 4.49e-97 307
MS.gene48246.t1 AT4G03930 43.411 387 206 5 103 478 129 513 1.49e-96 306
MS.gene48246.t1 AT5G09760 38.641 515 264 17 3 477 44 546 2.29e-96 306
MS.gene48246.t1 AT5G09760 38.447 515 265 16 3 477 70 572 4.43e-96 306
MS.gene48246.t1 AT5G20860 37.120 493 248 12 2 468 33 489 1.58e-94 300
MS.gene48246.t1 AT2G47030 44.510 337 167 6 160 478 243 577 2.73e-82 270
MS.gene48246.t1 AT3G62170 42.165 351 175 6 154 478 229 577 1.69e-79 263
MS.gene48246.t1 AT1G11370 48.594 249 125 2 174 419 38 286 4.91e-79 252
MS.gene48246.t1 AT2G47040 43.713 334 171 6 160 478 253 584 1.45e-78 261
MS.gene48246.t1 AT5G19730 37.864 309 165 6 183 478 87 381 2.63e-53 187
MS.gene48246.t1 AT3G17060 32.822 326 191 7 172 486 36 344 8.74e-49 174
MS.gene48246.t1 AT1G05310 33.934 333 189 8 159 478 74 388 3.92e-47 171
MS.gene48246.t1 AT2G36700 33.444 302 182 4 184 478 44 333 4.37e-47 169
MS.gene48246.t1 AT3G29090 35.816 282 159 4 182 451 7 278 5.33e-47 168
MS.gene48246.t1 AT2G21610 32.890 301 179 7 182 478 51 332 1.14e-44 163
MS.gene48246.t1 AT5G07430 31.081 370 229 8 119 478 2 355 1.19e-44 163
MS.gene48246.t1 AT2G21610 32.890 301 179 7 182 478 56 337 1.58e-44 162
MS.gene48246.t1 AT2G36710 32.558 301 181 6 188 478 97 385 6.27e-43 159
MS.gene48246.t1 AT5G55590 33.435 329 191 8 164 478 64 378 3.16e-42 157
MS.gene48246.t1 AT5G07420 32.390 318 191 7 169 478 54 355 4.57e-42 156
MS.gene48246.t1 AT5G47500 30.395 329 192 7 164 478 52 357 2.47e-41 154
MS.gene48246.t1 AT5G18990 33.441 311 177 7 182 478 29 323 4.99e-41 152
MS.gene48246.t1 AT2G19150 34.603 315 174 10 182 483 41 336 3.03e-40 150
MS.gene48246.t1 AT3G24130 33.099 284 163 6 182 451 34 304 2.39e-39 147
MS.gene48246.t1 AT5G55590 34.799 273 153 7 164 422 64 325 2.49e-39 147
MS.gene48246.t1 AT1G44980 42.021 188 103 2 162 343 54 241 3.26e-39 144
MS.gene48246.t1 AT5G61680 31.329 316 191 8 173 478 59 358 1.36e-38 146
MS.gene48246.t1 AT1G69940 28.834 326 207 7 162 478 46 355 2.68e-37 142
MS.gene48246.t1 AT5G07410 28.834 326 207 7 162 478 46 355 1.87e-36 140
MS.gene48246.t1 AT5G26810 32.372 312 184 8 182 478 34 333 3.09e-36 139
MS.gene48246.t1 AT2G47280 31.949 313 181 8 182 480 35 329 7.57e-34 132
MS.gene48246.t1 AT2G19150 35.317 252 139 7 182 422 41 279 1.33e-33 130
MS.gene48246.t1 AT3G49220 33.906 233 124 7 68 274 194 422 7.35e-32 128

Find 130 sgRNAs with CRISPR-Local

Find 193 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGCAAATATGTTGCAATTT+TGG 0.085785 4.2:+77463807 None:intergenic
GGTACGGTTGATTTCATATT+TGG 0.108393 4.2:-77463848 MS.gene48246:CDS
TACAAACAATGTAACATTTA+TGG 0.134217 4.2:-77463869 MS.gene48246:CDS
GATAAGTCTTAACAGAATTT+TGG 0.160141 4.2:+77463666 None:intergenic
GAATTTCTATTCACAATTCT+AGG 0.194647 4.2:-77463718 MS.gene48246:CDS
TACCTACCTTTGTTATTCTT+TGG 0.232922 4.2:+77464935 None:intergenic
GCATTAAGTACTTTATATTA+TGG 0.242821 4.2:-77463542 MS.gene48246:CDS
CCAAAGTTTAACTTCATTCA+TGG 0.264428 4.2:+77465543 None:intergenic
TGTTGTTGGAGATGGATTTA+TGG 0.279809 4.2:-77464038 MS.gene48246:CDS
TGGCTCAAGATATCACATTT+AGG 0.292215 4.2:-77464018 MS.gene48246:CDS
ACTGTATCAAATTTCATTGC+TGG 0.309927 4.2:-77463422 MS.gene48246:CDS
TTCACGTGAGTTACTACATT+TGG 0.320043 4.2:+77465357 None:intergenic
TACACACAAGGTTGAGGATT+TGG 0.321560 4.2:+77465508 None:intergenic
GTGATTGTGTTGACTTTGTT+TGG 0.331970 4.2:+77463776 None:intergenic
GAATAGAAATTCCAGTGTTT+TGG 0.338411 4.2:+77463729 None:intergenic
AAGCTTATGCTGTTTCTTCC+AGG 0.339637 4.2:+77462527 None:intergenic
TGATTTCTTCAAACTTTCAT+TGG 0.341515 4.2:-77465434 MS.gene48246:CDS
ATCCATCTGGTTGGTTAGAA+TGG 0.351047 4.2:-77463577 MS.gene48246:CDS
AATGATGATCATTCCCAAAA+AGG 0.353622 4.2:-77462394 MS.gene48246:CDS
TTCTAGGGTTACGGCTGCTT+CGG 0.355427 4.2:-77463702 MS.gene48246:CDS
AAGGTTGAGGATTTGGTGTT+TGG 0.356796 4.2:+77465515 None:intergenic
AATTCTGTTAAGACTTATCT+TGG 0.359832 4.2:-77463662 MS.gene48246:CDS
GGGAATCAAGTTAATCTTGT+TGG 0.369596 4.2:-77462588 MS.gene48246:CDS
TACTACAAACTTCATCTTCT+CGG 0.370790 4.2:-77465027 MS.gene48246:CDS
CAAGCTGTTGCATTGCGTTC+CGG 0.373938 4.2:-77463971 MS.gene48246:CDS
GACCATGGAAGCAATATTCT+AGG 0.374299 4.2:-77463637 MS.gene48246:CDS
GAACTTAGCGTCTCAATGAT+TGG 0.380622 4.2:+77462309 None:intergenic
CCCAAGGAATAATGCATGTT+AGG 0.382560 4.2:+77462670 None:intergenic
CATGCAAACACTCTTTCATT+AGG 0.385373 4.2:-77465382 MS.gene48246:CDS
CAATGAACAAGTTGAGATAA+AGG 0.386142 4.2:-77464885 MS.gene48246:CDS
ACCTAACATGCATTATTCCT+TGG 0.387628 4.2:-77462671 MS.gene48246:CDS
AACTTGAGAACACTTTGTTT+TGG 0.394932 4.2:+77465264 None:intergenic
TATTCACAATTCTAGGGTTA+CGG 0.397130 4.2:-77463711 MS.gene48246:CDS
TAGCAATTTAGTAACATTGT+TGG 0.401894 4.2:+77465141 None:intergenic
GTCTCAATGATTGGGAAATT+CGG 0.405148 4.2:+77462318 None:intergenic
CCTAACATGCATTATTCCTT+GGG 0.406540 4.2:-77462670 MS.gene48246:CDS
GGAATCAAGTTAATCTTGTT+GGG 0.409647 4.2:-77462587 MS.gene48246:CDS
ACTGATCTAGGAGTGGAATA+TGG 0.411308 4.2:-77462447 MS.gene48246:CDS
AGCTTTACATGTCTCAAGAT+TGG 0.413290 4.2:+77465210 None:intergenic
ATAATGCATGTTAGGTTCTC+TGG 0.415169 4.2:+77462678 None:intergenic
GGGAACCCATCTTTGTAGCT+AGG 0.418473 4.2:+77465079 None:intergenic
GATAGTTTGATTCATCCATC+TGG 0.426673 4.2:-77463590 MS.gene48246:CDS
GCAAAGAATATTATGTTGGT+TGG 0.435785 4.2:-77464863 MS.gene48246:CDS
AGTCAAGGTGTTACTGATCT+AGG 0.441226 4.2:-77462459 MS.gene48246:CDS
ATTCTTTGGTATAGCATTCA+CGG 0.447221 4.2:+77464949 None:intergenic
CCATGAATGAAGTTAAACTT+TGG 0.447619 4.2:-77465543 MS.gene48246:CDS
GAAGTTCTACGGTCATGGTT+TGG 0.448066 4.2:+77462424 None:intergenic
AACGCAATGCAACAGCTTGA+TGG 0.450785 4.2:+77463975 None:intergenic
TTTGCAGCTGTTGTTGGAGA+TGG 0.451928 4.2:-77464046 MS.gene48246:intron
GGCTGCTTCGGATTTGAAAC+CGG 0.452219 4.2:-77463690 MS.gene48246:CDS
AACTTAGCGTCTCAATGATT+GGG 0.452754 4.2:+77462310 None:intergenic
ACCATGACCGTAGAACTTCT+AGG 0.465551 4.2:-77462420 MS.gene48246:CDS
ACCTAGAAGTTCTACGGTCA+TGG 0.473530 4.2:+77462419 None:intergenic
ATCTTGCTTTGTGAAATAGA+TGG 0.474159 4.2:+77462498 None:intergenic
AATTTCTATTCACAATTCTA+GGG 0.475155 4.2:-77463717 MS.gene48246:CDS
ACATGCATTATTCCTTGGGT+TGG 0.476214 4.2:-77462666 MS.gene48246:CDS
CACCTGGCCTAACCCATGTT+GGG 0.479286 4.2:+77465059 None:intergenic
AATATTATGTTGGTTGGAGA+TGG 0.480645 4.2:-77464857 MS.gene48246:CDS
ATGAGTTTGTGTTGGTAAGA+AGG 0.482647 4.2:+77465469 None:intergenic
AAAGGCAAAGAATATTATGT+TGG 0.483951 4.2:-77464867 MS.gene48246:CDS
CCATTTGACACGGTTTCTTG+TGG 0.485738 4.2:+77463490 None:intergenic
GAGTATATGAACACTGGTCT+TGG 0.488203 4.2:-77463518 MS.gene48246:CDS
AAGCAAGATTATTCAGAAGC+TGG 0.488370 4.2:-77462484 MS.gene48246:CDS
TTGTGTTGACTTTGTTTGGA+GGG 0.490707 4.2:+77463780 None:intergenic
CAAGAACCTAGCTACAAAGA+TGG 0.493394 4.2:-77465085 MS.gene48246:CDS
CCACAAGAAACCGTGTCAAA+TGG 0.501895 4.2:-77463490 MS.gene48246:CDS
TACTACTGCAAATAAACAAA+GGG 0.505104 4.2:-77462608 MS.gene48246:CDS
GCCCTAGAATATTGCTTCCA+TGG 0.507314 4.2:+77463635 None:intergenic
AGCTTGATGGTTCTTTGCAC+CGG 0.510568 4.2:+77463988 None:intergenic
TCACCTGGCCTAACCCATGT+TGG 0.518597 4.2:+77465058 None:intergenic
ACGTAGTCGTGGCGAAAGAC+GGG 0.518740 4.2:-77465000 MS.gene48246:CDS
TGTAGTAACTTTCTATCACC+TGG 0.519105 4.2:+77465043 None:intergenic
TTTGTTTGATGAGTTTGTGT+TGG 0.521126 4.2:+77465461 None:intergenic
AACGTAGTCGTGGCGAAAGA+CGG 0.524269 4.2:-77465001 MS.gene48246:CDS
ACCGGTAGCAAAAGCGTTGG+AGG 0.524747 4.2:-77464815 MS.gene48246:CDS
AAATACAGACAAGTCTGATC+CGG 0.525381 4.2:+77463952 None:intergenic
ATGTTGGTTGGAGATGGTAT+TGG 0.525732 4.2:-77464851 MS.gene48246:CDS
TTCCCAACATGGGTTAGGCC+AGG 0.531406 4.2:-77465061 MS.gene48246:CDS
ACAATGTAACATTTATGGTA+CGG 0.531513 4.2:-77463864 MS.gene48246:CDS
AAGAAATACACACAAGGTTG+AGG 0.533464 4.2:+77465502 None:intergenic
ATGGGTTCCCAACATGGGTT+AGG 0.533950 4.2:-77465066 MS.gene48246:CDS
TGAGTTTGTGTTGGTAAGAA+GGG 0.534506 4.2:+77465470 None:intergenic
ACAAAGATGGGTTCCCAACA+TGG 0.535204 4.2:-77465072 MS.gene48246:CDS
TCTTTCGCCACGACTACGTT+AGG 0.545212 4.2:+77465004 None:intergenic
ACCTTCCGTTCTGCCACTGT+TGG 0.545587 4.2:-77464782 MS.gene48246:intron
TTATGCATCACAGTCTGCTA+TGG 0.546651 4.2:+77462257 None:intergenic
CATTCCACCTAGAAGTTCTA+CGG 0.550579 4.2:+77462413 None:intergenic
ATTGCTCTATGACTCCAGCT+TGG 0.551539 4.2:+77462630 None:intergenic
AATCTTGAGACATGTAAAGC+TGG 0.554422 4.2:-77465208 MS.gene48246:CDS
GCTAGCAGATGCTGTCAAGT+TGG 0.556073 4.2:-77462280 MS.gene48246:CDS
AAGAACCTAGCTACAAAGAT+GGG 0.557927 4.2:-77465084 MS.gene48246:CDS
GTTTGATTCATCCATCTGGT+TGG 0.562831 4.2:-77463586 MS.gene48246:CDS
CTCAAGTTGATGCTCAAACA+TGG 0.563888 4.2:-77465249 MS.gene48246:CDS
ATTGTGTTGACTTTGTTTGG+AGG 0.571190 4.2:+77463779 None:intergenic
ATTTCATTGCTGGAAATGCA+TGG 0.571250 4.2:-77463412 MS.gene48246:CDS
GTCGTGGCGAAAGACGGGTC+AGG 0.575618 4.2:-77464995 MS.gene48246:CDS
CTCCATTCTAACCAACCAGA+TGG 0.577867 4.2:+77463575 None:intergenic
ATTGGTAAAACTATAATCAC+CGG 0.579363 4.2:-77464833 MS.gene48246:CDS
ATACTACTGCAAATAAACAA+AGG 0.580807 4.2:-77462609 MS.gene48246:CDS
TGATCAGTGCTTCCAACCCA+AGG 0.584481 4.2:+77462654 None:intergenic
GAAGCACTGATCAACCAAGC+TGG 0.585971 4.2:-77462644 MS.gene48246:CDS
ACCAACAGTGGCAGAACGGA+AGG 0.586392 4.2:+77464781 None:intergenic
GATAACCTTGCCATTTGACA+CGG 0.589011 4.2:+77463480 None:intergenic
AGTGGCAGAACGGAAGGTTG+TGG 0.591491 4.2:+77464787 None:intergenic
ACAGATCCTAACCAAAACAC+TGG 0.592258 4.2:-77463740 MS.gene48246:CDS
ACCATGGAAGCAATATTCTA+GGG 0.592357 4.2:-77463636 MS.gene48246:CDS
CTAATGAAAGAGTGTTTGCA+TGG 0.596121 4.2:+77465383 None:intergenic
ATCACCGGTAGCAAAAGCGT+TGG 0.596826 4.2:-77464818 MS.gene48246:CDS
AGACTTATCTTGGAAGACCA+TGG 0.599466 4.2:-77463652 MS.gene48246:CDS
AAAGCTGGATTCTATGAGCT+TGG 0.600729 4.2:-77465193 MS.gene48246:CDS
TATGTTATACATGTGAAAGC+TGG 0.604818 4.2:-77464914 MS.gene48246:CDS
AGAAACCGTGTCAAATGGCA+AGG 0.607462 4.2:-77463485 MS.gene48246:CDS
TTCTCGGCCTAACGTAGTCG+TGG 0.607759 4.2:-77465011 MS.gene48246:CDS
TACCAAAGAATAACAAAGGT+AGG 0.608433 4.2:-77464937 MS.gene48246:CDS
GGTAGCAAAAGCGTTGGAGG+AGG 0.610712 4.2:-77464812 MS.gene48246:CDS
ATCACATTTAGGAACACTGC+CGG 0.612058 4.2:-77464007 MS.gene48246:CDS
GTAACATTGTTGGACATGAG+AGG 0.612493 4.2:+77465151 None:intergenic
CAAAGATGGGTTCCCAACAT+GGG 0.625227 4.2:-77465071 MS.gene48246:CDS
TAAATTGCTAAGTAACACAT+TGG 0.627948 4.2:-77465128 MS.gene48246:CDS
GCTATACCAAAGAATAACAA+AGG 0.633072 4.2:-77464941 MS.gene48246:CDS
TATGGAGAGTATATGAACAC+TGG 0.633586 4.2:-77463524 MS.gene48246:CDS
ATTCAGAAGCTGGAGAGTCA+AGG 0.635781 4.2:-77462474 MS.gene48246:CDS
ACGTACCAACAGTGGCAGAA+CGG 0.642943 4.2:+77464777 None:intergenic
GTTCAAGAAAGAGCACTCAA+AGG 0.644742 4.2:-77465406 MS.gene48246:CDS
GTCAACACAATCACTGCACA+AGG 0.651414 4.2:-77463767 MS.gene48246:CDS
AGGTGTTACTGATCTAGGAG+TGG 0.651725 4.2:-77462454 MS.gene48246:CDS
TCTGGTTGGTTAGAATGGAG+TGG 0.670039 4.2:-77463572 MS.gene48246:CDS
CAATGACTACGTACCAACAG+TGG 0.678439 4.2:+77464769 None:intergenic
TTGCTCAAGAAATACACACA+AGG 0.709189 4.2:+77465496 None:intergenic
ATGACCGTAGAACTTCTAGG+TGG 0.762413 4.2:-77462417 MS.gene48246:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATTATTACTAATTAAG+TGG + chr4.2:77464672-77464691 None:intergenic 10.0%
!! AAAAATTTAACATTAATAGA+AGG - chr4.2:77463214-77463233 MS.gene48246:intron 10.0%
!! AAAATTTAACATTAATAGAA+GGG - chr4.2:77463215-77463234 MS.gene48246:intron 10.0%
!! TATTAATGTTAAAAATTAAG+AGG + chr4.2:77463160-77463179 None:intergenic 10.0%
!!! ATATGAATTTTTATATTTTG+GGG + chr4.2:77463445-77463464 None:intergenic 10.0%
!!! TTAAAAACTTCTAATTTTTT+GGG - chr4.2:77463480-77463499 MS.gene48246:CDS 10.0%
!!! CGAAATTTTGATATATTTAT+AGG + chr4.2:77463259-77463278 None:intergenic 15.0%
!!! GTTAAAAACTTCTAATTTTT+TGG - chr4.2:77463479-77463498 MS.gene48246:CDS 15.0%
!!! TAAAAACTTCTAATTTTTTG+GGG - chr4.2:77463481-77463500 MS.gene48246:CDS 15.0%
!!! TATGAATTTTTATATTTTGG+GGG + chr4.2:77463444-77463463 None:intergenic 15.0%
!! AAATGCATTTGTAAAGTTAT+TGG - chr4.2:77464834-77464853 MS.gene48246:CDS 20.0%
!! AATTAAAGAGTAGATGTAAA+TGG + chr4.2:77464430-77464449 None:intergenic 20.0%
!! AATTTCTATTCACAATTCTA+GGG - chr4.2:77464081-77464100 MS.gene48246:intron 20.0%
!! GCATTAAGTACTTTATATTA+TGG - chr4.2:77464256-77464275 MS.gene48246:intron 20.0%
!! TACAAACAATGTAACATTTA+TGG - chr4.2:77463929-77463948 MS.gene48246:CDS 20.0%
!! TATGCAAGTTATAGTATTTA+TGG - chr4.2:77464754-77464773 MS.gene48246:intron 20.0%
!!! AAAAACTTCTAATTTTTTGG+GGG - chr4.2:77463482-77463501 MS.gene48246:CDS 20.0%
!!! AAGTCTTTGAAATTTTTTAG+AGG - chr4.2:77463623-77463642 MS.gene48246:CDS 20.0%
!!! AGTCTTTGAAATTTTTTAGA+GGG - chr4.2:77463624-77463643 MS.gene48246:CDS 20.0%
!!! TTTTATAGATGTAGTTTTGA+AGG - chr4.2:77463866-77463885 MS.gene48246:CDS 20.0%
! AAAGGCAAAGAATATTATGT+TGG - chr4.2:77462931-77462950 MS.gene48246:intron 25.0%
! AATTCTGTTAAGACTTATCT+TGG - chr4.2:77464136-77464155 MS.gene48246:intron 25.0%
! ACAATGTAACATTTATGGTA+CGG - chr4.2:77463934-77463953 MS.gene48246:CDS 25.0%
! ACACAAAAATCTCTTATGAA+GGG - chr4.2:77465017-77465036 MS.gene48246:CDS 25.0%
! AGTTGTGTTAATAGAGTAAA+TGG + chr4.2:77464712-77464731 None:intergenic 25.0%
! ATACTACTGCAAATAAACAA+AGG - chr4.2:77465189-77465208 MS.gene48246:CDS 25.0%
! ATTGGTAAAACTATAATCAC+CGG - chr4.2:77462965-77462984 MS.gene48246:intron 25.0%
! CATTGAGTGTATTCTATTAA+GGG + chr4.2:77463698-77463717 None:intergenic 25.0%
! GAATTTCTATTCACAATTCT+AGG - chr4.2:77464080-77464099 MS.gene48246:intron 25.0%
! TAAATTGCTAAGTAACACAT+TGG - chr4.2:77462670-77462689 MS.gene48246:CDS 25.0%
! TACACAAAAATCTCTTATGA+AGG - chr4.2:77465016-77465035 MS.gene48246:CDS 25.0%
! TACTACTGCAAATAAACAAA+GGG - chr4.2:77465190-77465209 MS.gene48246:CDS 25.0%
! TAGCAATTTAGTAACATTGT+TGG + chr4.2:77462660-77462679 None:intergenic 25.0%
! TATGAAACTTGAACAAGAAA+GGG - chr4.2:77465094-77465113 MS.gene48246:CDS 25.0%
! TCATTGAGTGTATTCTATTA+AGG + chr4.2:77463699-77463718 None:intergenic 25.0%
! TGATTTCTTCAAACTTTCAT+TGG - chr4.2:77462364-77462383 MS.gene48246:CDS 25.0%
! TTATGAAACTTGAACAAGAA+AGG - chr4.2:77465093-77465112 MS.gene48246:CDS 25.0%
!! AAGTGAAAATGGAAAACTTT+TGG - chr4.2:77463593-77463612 MS.gene48246:CDS 25.0%
!! AAGTTTTCCATTTTCACTTT+TGG + chr4.2:77463592-77463611 None:intergenic 25.0%
!! AATTAAGTGGTGTTAAAATG+AGG + chr4.2:77464659-77464678 None:intergenic 25.0%
!! AGAAGGGTTAAATATGTTTT+TGG - chr4.2:77463231-77463250 MS.gene48246:intron 25.0%
!! AGTGAAAATGGAAAACTTTT+GGG - chr4.2:77463594-77463613 MS.gene48246:CDS 25.0%
!! ATTTTCCACAATCACATTTT+TGG - chr4.2:77463295-77463314 MS.gene48246:intron 25.0%
!! GATAAGTCTTAACAGAATTT+TGG + chr4.2:77464135-77464154 None:intergenic 25.0%
!! TTATCAATCTTTTCATTACC+CGG + chr4.2:77464877-77464896 None:intergenic 25.0%
!! TTGCAAATATGTTGCAATTT+TGG + chr4.2:77463994-77464013 None:intergenic 25.0%
!!! TTTTAATAGAGGTACATAGA+TGG + chr4.2:77465248-77465267 None:intergenic 25.0%
AAAAATCTCTTATGAAGGGT+TGG - chr4.2:77465021-77465040 MS.gene48246:CDS 30.0%
AAAATCTCTTATGAAGGGTT+GGG - chr4.2:77465022-77465041 MS.gene48246:CDS 30.0%
AATGATGATCATTCCCAAAA+AGG - chr4.2:77465404-77465423 MS.gene48246:CDS 30.0%
ACCAAATCTACAAGAATAAG+TGG + chr4.2:77464986-77465005 None:intergenic 30.0%
ACCACTTATTCTTGTAGATT+TGG - chr4.2:77464982-77465001 MS.gene48246:CDS 30.0%
ACTGTATCAAATTTCATTGC+TGG - chr4.2:77464376-77464395 MS.gene48246:intron 30.0%
ACTTATTCTTGTAGATTTGG+TGG - chr4.2:77464985-77465004 MS.gene48246:CDS 30.0%
AGAGAAACCAAAAGTGAAAA+TGG - chr4.2:77463582-77463601 MS.gene48246:CDS 30.0%
ATCTTGCTTTGTGAAATAGA+TGG + chr4.2:77465303-77465322 None:intergenic 30.0%
ATTAGAGTACACATCTTGTT+AGG - chr4.2:77464940-77464959 MS.gene48246:CDS 30.0%
ATTCTTTGGTATAGCATTCA+CGG + chr4.2:77462852-77462871 None:intergenic 30.0%
GCAAAGAATATTATGTTGGT+TGG - chr4.2:77462935-77462954 MS.gene48246:intron 30.0%
GCTATACCAAAGAATAACAA+AGG - chr4.2:77462857-77462876 MS.gene48246:intron 30.0%
GGAATCAAGTTAATCTTGTT+GGG - chr4.2:77465211-77465230 MS.gene48246:CDS 30.0%
GTACCTCTATTAAAAATTCC+TGG - chr4.2:77465253-77465272 MS.gene48246:CDS 30.0%
TACCAAAGAATAACAAAGGT+AGG - chr4.2:77462861-77462880 MS.gene48246:intron 30.0%
TACTACAAACTTCATCTTCT+CGG - chr4.2:77462771-77462790 MS.gene48246:intron 30.0%
TATGTTATACATGTGAAAGC+TGG - chr4.2:77462884-77462903 MS.gene48246:intron 30.0%
TGTGTTAATAGAGTAAATGG+TGG + chr4.2:77464709-77464728 None:intergenic 30.0%
TTTGTTTGATGAGTTTGTGT+TGG + chr4.2:77462340-77462359 None:intergenic 30.0%
! AATATTATGTTGGTTGGAGA+TGG - chr4.2:77462941-77462960 MS.gene48246:intron 30.0%
! AGTTTTCTGAACATCCTTTT+TGG + chr4.2:77465421-77465440 None:intergenic 30.0%
! CAATGAACAAGTTGAGATAA+AGG - chr4.2:77462913-77462932 MS.gene48246:intron 30.0%
! CTTCCAGGAATTTTTAATAG+AGG + chr4.2:77465259-77465278 None:intergenic 30.0%
! GAATAGAAATTCCAGTGTTT+TGG + chr4.2:77464072-77464091 None:intergenic 30.0%
! GTGAAAATGGAAAACTTTTG+GGG - chr4.2:77463595-77463614 MS.gene48246:CDS 30.0%
! GTTTTCTGAACATCCTTTTT+GGG + chr4.2:77465420-77465439 None:intergenic 30.0%
!! AACTTGAGAACACTTTGTTT+TGG + chr4.2:77462537-77462556 None:intergenic 30.0%
!! TACCTACCTTTGTTATTCTT+TGG + chr4.2:77462866-77462885 None:intergenic 30.0%
!! TATTCACAATTCTAGGGTTA+CGG - chr4.2:77464087-77464106 MS.gene48246:intron 30.0%
AAATACAGACAAGTCTGATC+CGG + chr4.2:77463849-77463868 None:intergenic 35.0%
AAATCTCTTATGAAGGGTTG+GGG - chr4.2:77465023-77465042 MS.gene48246:CDS 35.0%
AACTTAGCGTCTCAATGATT+GGG + chr4.2:77465491-77465510 None:intergenic 35.0%
AAGAAATACACACAAGGTTG+AGG + chr4.2:77462299-77462318 None:intergenic 35.0%
AAGAACCTAGCTACAAAGAT+GGG - chr4.2:77462714-77462733 MS.gene48246:intron 35.0%
AAGCAAGATTATTCAGAAGC+TGG - chr4.2:77465314-77465333 MS.gene48246:CDS 35.0%
AATCTTGAGACATGTAAAGC+TGG - chr4.2:77462590-77462609 MS.gene48246:CDS 35.0%
ACCATGGAAGCAATATTCTA+GGG - chr4.2:77464162-77464181 MS.gene48246:intron 35.0%
ACCTAACATGCATTATTCCT+TGG - chr4.2:77465127-77465146 MS.gene48246:CDS 35.0%
AGAGACCAAAAATGTGATTG+TGG + chr4.2:77463303-77463322 None:intergenic 35.0%
AGCTTTACATGTCTCAAGAT+TGG + chr4.2:77462591-77462610 None:intergenic 35.0%
AGTAGATGTAAATGGCACTT+TGG + chr4.2:77464422-77464441 None:intergenic 35.0%
ATAATGCATGTTAGGTTCTC+TGG + chr4.2:77465123-77465142 None:intergenic 35.0%
ATTTCATTGCTGGAAATGCA+TGG - chr4.2:77464386-77464405 MS.gene48246:intron 35.0%
CATGCAAACACTCTTTCATT+AGG - chr4.2:77462416-77462435 MS.gene48246:CDS 35.0%
CCTAACATGCATTATTCCTT+GGG - chr4.2:77465128-77465147 MS.gene48246:CDS 35.0%
GATAGTTTGATTCATCCATC+TGG - chr4.2:77464208-77464227 MS.gene48246:intron 35.0%
GGGAATCAAGTTAATCTTGT+TGG - chr4.2:77465210-77465229 MS.gene48246:CDS 35.0%
GTCTCAATGATTGGGAAATT+CGG + chr4.2:77465483-77465502 None:intergenic 35.0%
TGGCTCAAGATATCACATTT+AGG - chr4.2:77463780-77463799 MS.gene48246:CDS 35.0%
TGTAGTAACTTTCTATCACC+TGG + chr4.2:77462758-77462777 None:intergenic 35.0%
TTCACGTGAGTTACTACATT+TGG + chr4.2:77462444-77462463 None:intergenic 35.0%
TTGCTCAAGAAATACACACA+AGG + chr4.2:77462305-77462324 None:intergenic 35.0%
! ATGAGTTTGTGTTGGTAAGA+AGG + chr4.2:77462332-77462351 None:intergenic 35.0%
! GGTACGGTTGATTTCATATT+TGG - chr4.2:77463950-77463969 MS.gene48246:CDS 35.0%
! GTCTTAACAGAATTTTGGAC+CGG + chr4.2:77464130-77464149 None:intergenic 35.0%
! TATGGAGAGTATATGAACAC+TGG - chr4.2:77464274-77464293 MS.gene48246:intron 35.0%
! TGAGTTTGTGTTGGTAAGAA+GGG + chr4.2:77462331-77462350 None:intergenic 35.0%
! TGTTGTTGGAGATGGATTTA+TGG - chr4.2:77463760-77463779 MS.gene48246:CDS 35.0%
!! ATTGTGTTGACTTTGTTTGG+AGG + chr4.2:77464022-77464041 None:intergenic 35.0%
!! CTAATGAAAGAGTGTTTGCA+TGG + chr4.2:77462418-77462437 None:intergenic 35.0%
!! GTGATTGTGTTGACTTTGTT+TGG + chr4.2:77464025-77464044 None:intergenic 35.0%
!! TAGAGTAAATGGTGGTGTTT+TGG + chr4.2:77464701-77464720 None:intergenic 35.0%
!! TTGTGTTGACTTTGTTTGGA+GGG + chr4.2:77464021-77464040 None:intergenic 35.0%
!!! AACACTTTGTTTTGGTGTCT+AGG + chr4.2:77462529-77462548 None:intergenic 35.0%
!!! ACACTTTGTTTTGGTGTCTA+GGG + chr4.2:77462528-77462547 None:intergenic 35.0%
!!! GAAATTCCAGTGTTTTGGTT+AGG + chr4.2:77464067-77464086 None:intergenic 35.0%
AAAGCTGGATTCTATGAGCT+TGG - chr4.2:77462605-77462624 MS.gene48246:CDS 40.0%
ACAGATCCTAACCAAAACAC+TGG - chr4.2:77464058-77464077 MS.gene48246:intron 40.0%
ACATGCATTATTCCTTGGGT+TGG - chr4.2:77465132-77465151 MS.gene48246:CDS 40.0%
ACTCACGTGAAAAATCAGCT+TGG - chr4.2:77462453-77462472 MS.gene48246:CDS 40.0%
ACTGATCTAGGAGTGGAATA+TGG - chr4.2:77465351-77465370 MS.gene48246:CDS 40.0%
AGACTTATCTTGGAAGACCA+TGG - chr4.2:77464146-77464165 MS.gene48246:intron 40.0%
ATCACATTTAGGAACACTGC+CGG - chr4.2:77463791-77463810 MS.gene48246:CDS 40.0%
CAAGAACCTAGCTACAAAGA+TGG - chr4.2:77462713-77462732 MS.gene48246:intron 40.0%
CATTCCACCTAGAAGTTCTA+CGG + chr4.2:77465388-77465407 None:intergenic 40.0%
CCCAAGGAATAATGCATGTT+AGG + chr4.2:77465131-77465150 None:intergenic 40.0%
CTCACGTGAAAAATCAGCTT+GGG - chr4.2:77462454-77462473 MS.gene48246:CDS 40.0%
GAACTTAGCGTCTCAATGAT+TGG + chr4.2:77465492-77465511 None:intergenic 40.0%
GACCATGGAAGCAATATTCT+AGG - chr4.2:77464161-77464180 MS.gene48246:intron 40.0%
GATCTTAAATCTAGACCTCC+GGG - chr4.2:77464856-77464875 MS.gene48246:CDS 40.0%
GGATCTTAAATCTAGACCTC+CGG - chr4.2:77464855-77464874 MS.gene48246:CDS 40.0%
GTAAATGGCACTTTGGTTAC+AGG + chr4.2:77464415-77464434 None:intergenic 40.0%
GTAACATTGTTGGACATGAG+AGG + chr4.2:77462650-77462669 None:intergenic 40.0%
GTTTGATTCATCCATCTGGT+TGG - chr4.2:77464212-77464231 MS.gene48246:intron 40.0%
TACACACAAGGTTGAGGATT+TGG + chr4.2:77462293-77462312 None:intergenic 40.0%
TTATGCATCACAGTCTGCTA+TGG + chr4.2:77465544-77465563 None:intergenic 40.0%
! ATCCATCTGGTTGGTTAGAA+TGG - chr4.2:77464221-77464240 MS.gene48246:intron 40.0%
! ATGTTGGTTGGAGATGGTAT+TGG - chr4.2:77462947-77462966 MS.gene48246:intron 40.0%
! GAGTATATGAACACTGGTCT+TGG - chr4.2:77464280-77464299 MS.gene48246:intron 40.0%
! GATAACCTTGCCATTTGACA+CGG + chr4.2:77464321-77464340 None:intergenic 40.0%
! TCAATCTTTTCATTACCCGG+AGG + chr4.2:77464874-77464893 None:intergenic 40.0%
! TGTCTTTTTGCAGCTGTTGT+TGG - chr4.2:77463746-77463765 MS.gene48246:CDS 40.0%
!! AAGCTTATGCTGTTTCTTCC+AGG + chr4.2:77465274-77465293 None:intergenic 40.0%
!! AAGGTTGAGGATTTGGTGTT+TGG + chr4.2:77462286-77462305 None:intergenic 40.0%
!! AGTCAAGGTGTTACTGATCT+AGG - chr4.2:77465339-77465358 MS.gene48246:CDS 40.0%
!! CTCAAGTTGATGCTCAAACA+TGG - chr4.2:77462549-77462568 MS.gene48246:CDS 40.0%
!! GTTCAAGAAAGAGCACTCAA+AGG - chr4.2:77462392-77462411 MS.gene48246:CDS 40.0%
!!! CTAGGGTTTTGTTGAGCTTA+AGG + chr4.2:77462511-77462530 None:intergenic 40.0%
!!! GGTTTTGTTGAGCTTAAGGA+TGG + chr4.2:77462507-77462526 None:intergenic 40.0%
AACGCAATGCAACAGCTTGA+TGG + chr4.2:77463826-77463845 None:intergenic 45.0%
ACCATGACCGTAGAACTTCT+AGG - chr4.2:77465378-77465397 MS.gene48246:CDS 45.0%
ACCTAGAAGTTCTACGGTCA+TGG + chr4.2:77465382-77465401 None:intergenic 45.0%
AGAAACCGTGTCAAATGGCA+AGG - chr4.2:77464313-77464332 MS.gene48246:intron 45.0%
ATGACCGTAGAACTTCTAGG+TGG - chr4.2:77465381-77465400 MS.gene48246:CDS 45.0%
ATTCAGAAGCTGGAGAGTCA+AGG - chr4.2:77465324-77465343 MS.gene48246:CDS 45.0%
ATTGCTCTATGACTCCAGCT+TGG + chr4.2:77465171-77465190 None:intergenic 45.0%
CAATGACTACGTACCAACAG+TGG + chr4.2:77463032-77463051 None:intergenic 45.0%
CCACAAGAAACCGTGTCAAA+TGG - chr4.2:77464308-77464327 MS.gene48246:intron 45.0%
CCATTTGACACGGTTTCTTG+TGG + chr4.2:77464311-77464330 None:intergenic 45.0%
CTCCATTCTAACCAACCAGA+TGG + chr4.2:77464226-77464245 None:intergenic 45.0%
GAAGTTCTACGGTCATGGTT+TGG + chr4.2:77465377-77465396 None:intergenic 45.0%
GCCCTAGAATATTGCTTCCA+TGG + chr4.2:77464166-77464185 None:intergenic 45.0%
GTCAACACAATCACTGCACA+AGG - chr4.2:77464031-77464050 MS.gene48246:CDS 45.0%
TTTGCAGCTGTTGTTGGAGA+TGG - chr4.2:77463752-77463771 MS.gene48246:CDS 45.0%
! ACAAAGATGGGTTCCCAACA+TGG - chr4.2:77462726-77462745 MS.gene48246:intron 45.0%
! AGCTTGATGGTTCTTTGCAC+CGG + chr4.2:77463813-77463832 None:intergenic 45.0%
! CAAAGATGGGTTCCCAACAT+GGG - chr4.2:77462727-77462746 MS.gene48246:intron 45.0%
! TCTGGTTGGTTAGAATGGAG+TGG - chr4.2:77464226-77464245 MS.gene48246:intron 45.0%
!! AGGTGTTACTGATCTAGGAG+TGG - chr4.2:77465344-77465363 MS.gene48246:CDS 45.0%
!!! AAATATGAATTTTTATATTT+TGG + chr4.2:77463447-77463466 None:intergenic 5.0%
!!! AATATGAATTTTTATATTTT+GGG + chr4.2:77463446-77463465 None:intergenic 5.0%
AACGTAGTCGTGGCGAAAGA+CGG - chr4.2:77462797-77462816 MS.gene48246:intron 50.0%
ACGTACCAACAGTGGCAGAA+CGG + chr4.2:77463024-77463043 None:intergenic 50.0%
ATGGGTTCCCAACATGGGTT+AGG - chr4.2:77462732-77462751 MS.gene48246:intron 50.0%
CAAGCTGTTGCATTGCGTTC+CGG - chr4.2:77463827-77463846 MS.gene48246:CDS 50.0%
GCTAGCAGATGCTGTCAAGT+TGG - chr4.2:77465518-77465537 MS.gene48246:CDS 50.0%
GGGAACCCATCTTTGTAGCT+AGG + chr4.2:77462722-77462741 None:intergenic 50.0%
TCTTTCGCCACGACTACGTT+AGG + chr4.2:77462797-77462816 None:intergenic 50.0%
TGATCAGTGCTTCCAACCCA+AGG + chr4.2:77465147-77465166 None:intergenic 50.0%
! ATCACCGGTAGCAAAAGCGT+TGG - chr4.2:77462980-77462999 MS.gene48246:intron 50.0%
! GGCTGCTTCGGATTTGAAAC+CGG - chr4.2:77464108-77464127 MS.gene48246:intron 50.0%
! TCCTCCAACGCTTTTGCTAC+CGG + chr4.2:77462987-77463006 None:intergenic 50.0%
!! GAAGCACTGATCAACCAAGC+TGG - chr4.2:77465154-77465173 MS.gene48246:CDS 50.0%
!! TTCTAGGGTTACGGCTGCTT+CGG - chr4.2:77464096-77464115 MS.gene48246:intron 50.0%
ACCAACAGTGGCAGAACGGA+AGG + chr4.2:77463020-77463039 None:intergenic 55.0%
ACCTTCCGTTCTGCCACTGT+TGG - chr4.2:77463016-77463035 MS.gene48246:intron 55.0%
ACGTAGTCGTGGCGAAAGAC+GGG - chr4.2:77462798-77462817 MS.gene48246:intron 55.0%
AGTGGCAGAACGGAAGGTTG+TGG + chr4.2:77463014-77463033 None:intergenic 55.0%
CACCTGGCCTAACCCATGTT+GGG + chr4.2:77462742-77462761 None:intergenic 55.0%
TCACCTGGCCTAACCCATGT+TGG + chr4.2:77462743-77462762 None:intergenic 55.0%
TTCTCGGCCTAACGTAGTCG+TGG - chr4.2:77462787-77462806 MS.gene48246:intron 55.0%
!! ACCGGTAGCAAAAGCGTTGG+AGG - chr4.2:77462983-77463002 MS.gene48246:intron 55.0%
!! GGTAGCAAAAGCGTTGGAGG+AGG - chr4.2:77462986-77463005 MS.gene48246:intron 55.0%
!! TTCCCAACATGGGTTAGGCC+AGG - chr4.2:77462737-77462756 MS.gene48246:intron 55.0%
GTCGTGGCGAAAGACGGGTC+AGG - chr4.2:77462803-77462822 MS.gene48246:intron 65.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 77462257 77465563 77462257 ID=MS.gene48246
chr4.2 mRNA 77462257 77465563 77462257 ID=MS.gene48246.t1;Parent=MS.gene48246
chr4.2 exon 77464783 77465563 77464783 ID=MS.gene48246.t1.exon1;Parent=MS.gene48246.t1
chr4.2 CDS 77464783 77465563 77464783 ID=cds.MS.gene48246.t1;Parent=MS.gene48246.t1
chr4.2 exon 77463406 77464061 77463406 ID=MS.gene48246.t1.exon2;Parent=MS.gene48246.t1
chr4.2 CDS 77463406 77464061 77463406 ID=cds.MS.gene48246.t1;Parent=MS.gene48246.t1
chr4.2 exon 77462257 77462709 77462257 ID=MS.gene48246.t1.exon3;Parent=MS.gene48246.t1
chr4.2 CDS 77462257 77462709 77462257 ID=cds.MS.gene48246.t1;Parent=MS.gene48246.t1
Gene Sequence

>MS.gene48246

ATGAATGAAGTTAAACTTTGGTGTAACCAAACACCAAATCCTCAACCTTGTGTGTATTTCTTGAGCAATAACCCTTCTTACCAACACAAACTCATCAAACAAAAAGTTGATTTCTTCAAACTTTCATTGGAACTTGTTCAAGAAAGAGCACTCAAAGGCCATGCAAACACTCTTTCATTAGGCTCCAAATGTAGTAACTCACGTGAAAAATCAGCTTGGGCTGATTGTGTTGAGCTATATGAACAAACCATCCTTAAGCTCAACAAAACCCTAGACACCAAAACAAAGTGTTCTCAAGTTGATGCTCAAACATGGCTAAGCACTGCTCTCACCAATCTTGAGACATGTAAAGCTGGATTCTATGAGCTTGGTGTTCAAGATTATATTCTTCCTCTCATGTCCAACAATGTTACTAAATTGCTAAGTAACACATTGGCTCTTAACAAAGTTCAATATCAAGAACCTAGCTACAAAGATGGGTTCCCAACATGGGTTAGGCCAGGTGATAGAAAGTTACTACAAACTTCATCTTCTCGGCCTAACGTAGTCGTGGCGAAAGACGGGTCAGGAAAGTACACAACTGTTAGTGCAGCCGTGAATGCTATACCAAAGAATAACAAAGGTAGGTATGTTATACATGTGAAAGCTGGTGTCTACAATGAACAAGTTGAGATAAAGGCAAAGAATATTATGTTGGTTGGAGATGGTATTGGTAAAACTATAATCACCGGTAGCAAAAGCGTTGGAGGAGGAACCACAACCTTCCGTTCTGCCACTGTTGGTACGTAGTCATTGTTGAAAAAAATTATTTATTGATACAAGTTTGAGATAAAATATGAAATGTTGCTTATTATGTCTTTAATTGTTATATGTGTGTGTGTATCTAGATTAAAACATTCTCCTCTTAATTTTTAACATTAATAGAATACACAAAACTTTGACATTAATAGAATACACAAAAATTTAACATTAATAGAAGGGTTAAATATGTTTTTGGTTCCTATAAATATATCAAAATTTCGTTTTAGTTCTTCTAAAATTTTCCACAATCACATTTTTGGTCTCTATTTTTAATTCAACTCATGTATTTTTAATAAAATTATGCAGAAAAACGTATAAAATATTAAAAAAAATCTCTAAATATTTTTTTTTTAACAAATCATGAATTAAATATAAATTTTTAACCCCCAAAATATAAAAATTCATATTTAATTCATGTTTAGTTAAAAACTTCTAATTTTTTGGGGGAGTTTCTTATGATATTATAAAGTTTTCAGCAAAATTTTATTCAAAACTATGAATTTTACATATGAATTTACTTAAAAAGAGAAACCAAAAGTGAAAATGGAAAACTTTTGGGGACTGAAAGTCTTTGAAATTTTTTAGAGGGACTAAAATGAAAAACTGATCTATTTATATAGACTAAAGACATATTTAACCCTTAATAGAATACACTCAATGACTAGAAAACTAACTAATATATATATTATGTCTTTTTGCAGCTGTTGTTGGAGATGGATTTATGGCTCAAGATATCACATTTAGGAACACTGCCGGTGCAAAGAACCATCAAGCTGTTGCATTGCGTTCCGGATCAGACTTGTCTGTATTTTATAGATGTAGTTTTGAAGGTTTTCAAGACACATTATATGTGCATTCAGATAGACAATTCTACAAACAATGTAACATTTATGGTACGGTTGATTTCATATTTGGCAATGCTGCTGCAGTGTTCCAAAATTGCAACATATTTGCAAGAAACCCTCCAAACAAAGTCAACACAATCACTGCACAAGGTAGAACAGATCCTAACCAAAACACTGGAATTTCTATTCACAATTCTAGGGTTACGGCTGCTTCGGATTTGAAACCGGTCCAAAATTCTGTTAAGACTTATCTTGGAAGACCATGGAAGCAATATTCTAGGGCAGTTTTTATGAAGACTTCTCTTGATAGTTTGATTCATCCATCTGGTTGGTTAGAATGGAGTGGTAATTTTGCATTAAGTACTTTATATTATGGAGAGTATATGAACACTGGTCTTGGTTCTTCCACAAGAAACCGTGTCAAATGGCAAGGTTATCATGTGATAACAAGTGCTTCTGAAGCATCAAAGTTCACTGTATCAAATTTCATTGCTGGAAATGCATGGTTACCTGTAACCAAAGTGCCATTTACATCTACTCTTTAATTGAGTAGACGTACTTACATTGATAGTTTATTGATTTAATTGTTGATTGTTTGATTTGTTTCAATATTCATTTTATTCTTTGATAATTGTTTAGTAATGTAACATTACAACTTGAAATCTATTGTGTATAATTTGTCGCTATCTTAATTTTTTGTATTTGATTGTTTTGTTGTTAGATTGTAATAATGATCATAAAGATGTTGCTTTGCCTCATTTTAACACCACTTAATTAGTAATAATTTTTGTCAAACCAAAACACCACCATTTACTCTATTAACACAACTGCTCGTCTAATTTAATTTAAAATATGCAAGTTATAGTATTTATGGTTGACTTAAAAAACAACACACGTGTGAAAAAAGAGCAGTACTCATTTTGTTATTGTCAAATGCATTTGTAAAGTTATTGGATCTTAAATCTAGACCTCCGGGTAATGAAAAGATTGATAACAAAAATCAAATTCGAGAACTTTAGAGAAGTGCACTTCAAATAATTAGAGTACACATCTTGTTAGGAATTGACTCGTTCATGAAAACCACTTATTCTTGTAGATTTGGTGGCATTTTTTTACACAAAAATCTCTTATGAAGGGTTGGGGCATTTCAAATTCTACTAACAAAGATGCATGTGAGAATTATCTATTTCTTATGAAACTTGAACAAGAAAGGGCTGCCAGAGAACCTAACATGCATTATTCCTTGGGTTGGAAGCACTGATCAACCAAGCTGGAGTCATAGAGCAATACTACTGCAAATAAACAAAGGGAATCAAGTTAATCTTGTTGGGTACATCTTCAACCATCTATGTACCTCTATTAAAAATTCCTGGAAGAAACAGCATAAGCTTCTTCAACCATCTATTTCACAAAGCAAGATTATTCAGAAGCTGGAGAGTCAAGGTGTTACTGATCTAGGAGTGGAATATGGCCAAACCATGACCGTAGAACTTCTAGGTGGAATGATGATCATTCCCAAAAAGGATGTTCAGAAAACTACTGCTTTTTCTATTCAGAAAGACATAAGTCATATGATTCCGAATTTCCCAATCATTGAGACGCTAAGTTCACAAGAGCTAGCAGATGCTGTCAAGTTGGCCATAGCAGACTGTGATGCATAA
Protein sequence

>MS.gene48246.t1

MNEVKLWCNQTPNPQPCVYFLSNNPSYQHKLIKQKVDFFKLSLELVQERALKGHANTLSLGSKCSNSREKSAWADCVELYEQTILKLNKTLDTKTKCSQVDAQTWLSTALTNLETCKAGFYELGVQDYILPLMSNNVTKLLSNTLALNKVQYQEPSYKDGFPTWVRPGDRKLLQTSSSRPNVVVAKDGSGKYTTVSAAVNAIPKNNKGRYVIHVKAGVYNEQVEIKAKNIMLVGDGIGKTIITGSKSVGGGTTTFRSATVAVVGDGFMAQDITFRNTAGAKNHQAVALRSGSDLSVFYRCSFEGFQDTLYVHSDRQFYKQCNIYGTVDFIFGNAAAVFQNCNIFARNPPNKVNTITAQGRTDPNQNTGISIHNSRVTAASDLKPVQNSVKTYLGRPWKQYSRAVFMKTSLDSLIHPSGWLEWSGNFALSTLYYGEYMNTGLGSSTRNRVKWQGYHVITSASEASKFTVSNFIAGNAWLPKGLPENLTCIIPWVGSTDQPSWSHRAILLQINKGNQVNLVGYIFNHLCTSIKNSWKKQHKLLQPSISQSKIIQKLESQGVTDLGVEYGQTMTVELLGGMMIIPKKDVQKTTAFSIQKDISHMIPNFPIIETLSSQELADAVKLAIADCDA