AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene48247

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene48247.t1 MTR_4g025690 97.137 489 14 0 1 489 25 513 0.0 994
MS.gene48247.t1 MTR_4g025730 74.338 491 117 5 1 489 26 509 0.0 756
MS.gene48247.t1 MTR_8g104655 50.584 514 220 10 5 489 33 541 1.20e-179 514
MS.gene48247.t1 MTR_3g033690 53.493 501 212 10 4 489 32 526 4.00e-179 513
MS.gene48247.t1 MTR_3g008640 53.386 502 212 11 4 489 32 527 7.59e-178 509
MS.gene48247.t1 MTR_3g008720 53.386 502 212 11 4 489 32 527 2.63e-177 508
MS.gene48247.t1 MTR_4g035980 51.287 505 226 9 4 489 36 539 3.77e-177 508
MS.gene48247.t1 MTR_7g053360 53.386 502 212 11 4 489 32 527 5.95e-177 507
MS.gene48247.t1 MTR_3g008700 52.778 504 216 11 2 489 30 527 1.04e-175 504
MS.gene48247.t1 MTR_7g108905 53.273 443 181 8 69 485 110 552 4.87e-160 465
MS.gene48247.t1 MTR_8g104645 62.059 340 120 4 159 489 33 372 3.29e-155 446
MS.gene48247.t1 MTR_4g025670 53.052 426 181 8 72 484 65 484 1.29e-153 446
MS.gene48247.t1 MTR_4g087980 51.242 443 191 6 66 489 80 516 9.03e-153 445
MS.gene48247.t1 MTR_8g104580 45.224 513 216 11 5 489 25 500 3.36e-151 441
MS.gene48247.t1 MTR_8g023310 45.992 524 243 10 4 489 68 589 5.96e-148 436
MS.gene48247.t1 MTR_1g008140 43.916 526 247 10 8 489 86 607 6.18e-148 436
MS.gene48247.t1 MTR_2g044880 50.112 447 203 5 61 489 81 525 1.83e-147 432
MS.gene48247.t1 MTR_7g050980 51.549 452 185 10 63 489 113 555 1.60e-146 431
MS.gene48247.t1 MTR_3g091380 44.359 523 243 10 8 489 82 597 1.49e-145 430
MS.gene48247.t1 MTR_7g050870 51.678 447 192 9 63 489 112 554 8.38e-145 426
MS.gene48247.t1 MTR_1g083520 48.425 508 217 13 8 485 32 524 1.33e-144 425
MS.gene48247.t1 MTR_1g088020 47.893 522 213 16 24 489 37 555 1.13e-140 416
MS.gene48247.t1 MTR_7g112580 48.904 456 188 10 66 489 100 542 1.57e-138 410
MS.gene48247.t1 MTR_7g108890 45.624 537 223 14 8 489 40 562 7.58e-138 409
MS.gene48247.t1 MTR_1g088225 47.701 522 212 17 24 489 37 553 1.26e-137 408
MS.gene48247.t1 MTR_1g054880 50.108 465 183 12 72 489 96 558 3.33e-137 407
MS.gene48247.t1 MTR_7g050950 49.220 449 199 10 63 489 112 553 1.78e-136 405
MS.gene48247.t1 MTR_7g108900 45.962 520 223 15 25 489 44 560 2.52e-134 400
MS.gene48247.t1 MTR_4g087970 46.067 445 213 5 67 489 78 517 2.75e-133 395
MS.gene48247.t1 MTR_8g042910 47.571 494 196 13 51 489 127 612 8.52e-132 395
MS.gene48247.t1 MTR_7g108875 43.902 533 238 13 8 489 42 564 1.69e-131 393
MS.gene48247.t1 MTR_8g042900 43.883 515 251 13 4 489 76 581 2.24e-131 393
MS.gene48247.t1 MTR_3g010780 43.827 486 233 11 34 489 99 574 1.10e-127 383
MS.gene48247.t1 MTR_2g044810 41.600 500 270 9 1 485 38 530 7.93e-124 372
MS.gene48247.t1 MTR_4g124550 42.412 514 257 12 4 484 81 588 2.10e-123 373
MS.gene48247.t1 MTR_0458s0030 65.019 263 90 2 228 489 53 314 1.89e-122 360
MS.gene48247.t1 MTR_1g088040 65.019 263 90 2 228 489 53 314 1.89e-122 360
MS.gene48247.t1 MTR_3g010770 57.643 314 126 4 182 489 263 575 5.77e-122 369
MS.gene48247.t1 MTR_4g124520 47.030 404 188 10 100 485 122 517 1.29e-119 360
MS.gene48247.t1 MTR_8g023150 42.054 516 259 12 4 489 77 582 2.59e-119 362
MS.gene48247.t1 MTR_0618s0020 67.544 228 73 1 263 489 1 228 4.40e-116 341
MS.gene48247.t1 MTR_1g086190 39.836 487 253 11 37 485 75 559 2.68e-110 338
MS.gene48247.t1 MTR_1g086210 42.317 423 205 8 101 485 144 565 6.67e-110 337
MS.gene48247.t1 MTR_8g093460 38.964 521 263 15 2 483 41 545 2.85e-104 322
MS.gene48247.t1 MTR_2g044660 48.632 329 161 5 159 484 304 627 1.01e-102 321
MS.gene48247.t1 MTR_3g095920 39.265 517 267 14 1 478 45 553 6.65e-102 317
MS.gene48247.t1 MTR_1g083510 37.500 536 273 16 4 487 56 581 7.83e-101 314
MS.gene48247.t1 MTR_7g110490 39.316 468 227 12 70 484 106 569 4.20e-100 312
MS.gene48247.t1 MTR_7g110510 39.316 468 227 12 70 484 106 569 4.20e-100 312
MS.gene48247.t1 MTR_7g112590 36.905 504 263 13 32 486 83 580 1.08e-99 311
MS.gene48247.t1 MTR_7g110440 37.607 468 235 10 70 484 106 569 1.29e-99 311
MS.gene48247.t1 MTR_7g110560 38.936 470 251 11 48 485 80 545 9.69e-99 308
MS.gene48247.t1 MTR_1g083470 35.714 532 275 15 1 485 55 566 1.53e-97 306
MS.gene48247.t1 MTR_1g083500 35.714 532 275 15 1 485 55 566 1.53e-97 306
MS.gene48247.t1 MTR_1g083490 35.361 526 293 15 1 489 60 575 1.21e-95 301
MS.gene48247.t1 MTR_4g005170 38.053 452 245 12 66 485 123 571 2.28e-95 301
MS.gene48247.t1 MTR_4g005180 37.013 462 236 12 66 485 105 553 1.68e-93 295
MS.gene48247.t1 MTR_2g044810 53.846 247 112 2 240 485 216 461 2.67e-92 288
MS.gene48247.t1 MTR_2g436040 33.333 507 308 11 4 486 43 543 3.78e-92 293
MS.gene48247.t1 MTR_4g120290 35.824 522 283 13 4 485 59 568 7.32e-92 291
MS.gene48247.t1 MTR_8g088610 43.976 332 177 7 161 486 45 373 1.23e-90 281
MS.gene48247.t1 MTR_4g120280 35.837 466 245 10 65 486 114 569 1.70e-90 287
MS.gene48247.t1 MTR_2g436050 36.170 470 268 11 48 486 93 561 1.86e-87 279
MS.gene48247.t1 MTR_5g083620 41.716 338 181 7 159 485 20 352 7.41e-86 268
MS.gene48247.t1 MTR_7g110420 36.059 477 244 12 70 489 106 578 8.06e-86 275
MS.gene48247.t1 MTR_0458s0010 38.964 521 224 19 24 489 37 518 1.25e-84 270
MS.gene48247.t1 MTR_7g110430 32.976 467 224 10 70 484 106 535 2.67e-79 257
MS.gene48247.t1 MTR_2g105560 40.385 312 171 5 181 489 40 339 8.63e-74 236
MS.gene48247.t1 MTR_7g107180 40.317 315 175 5 178 489 3 307 1.56e-72 232
MS.gene48247.t1 MTR_6g024165 39.875 321 168 8 169 478 28 334 2.35e-67 220
MS.gene48247.t1 MTR_2g039530 42.763 304 157 6 158 456 28 319 2.33e-64 212
MS.gene48247.t1 MTR_7g111560 30.297 505 313 16 8 487 64 554 2.23e-63 216
MS.gene48247.t1 MTR_1g086540 36.646 322 178 6 169 478 37 344 2.83e-57 194
MS.gene48247.t1 MTR_8g102380 38.158 304 159 6 190 478 88 377 1.95e-55 190
MS.gene48247.t1 MTR_6g088810 39.145 304 154 7 190 478 90 377 7.81e-55 188
MS.gene48247.t1 MTR_6g012800 34.356 326 179 9 182 487 6 316 1.60e-52 180
MS.gene48247.t1 MTR_6g042660 35.764 288 157 7 171 451 30 296 8.44e-49 171
MS.gene48247.t1 MTR_7g092780 32.132 333 195 7 159 478 73 387 3.35e-47 168
MS.gene48247.t1 MTR_8g102380 40.726 248 121 6 190 422 88 324 4.02e-47 166
MS.gene48247.t1 MTR_7g011530 33.230 322 184 8 184 487 8 316 5.08e-47 166
MS.gene48247.t1 MTR_5g083590 32.143 308 155 5 182 485 7 264 2.87e-46 162
MS.gene48247.t1 MTR_5g095490 33.876 307 171 6 188 478 67 357 6.19e-45 161
MS.gene48247.t1 MTR_6g042690 34.859 284 152 8 182 451 39 303 3.56e-44 158
MS.gene48247.t1 MTR_4g124740 34.091 308 170 7 182 478 54 339 1.26e-43 157
MS.gene48247.t1 MTR_2g033970 32.390 318 191 6 172 480 46 348 3.30e-43 156
MS.gene48247.t1 MTR_7g102600 32.459 305 184 6 184 478 101 393 6.14e-43 157
MS.gene48247.t1 MTR_1g033050 32.982 285 168 5 177 451 13 284 1.22e-42 154
MS.gene48247.t1 MTR_5g012600 31.529 314 187 7 182 481 63 362 2.17e-40 149
MS.gene48247.t1 MTR_5g088300 32.215 298 183 5 182 478 37 316 6.86e-39 144
MS.gene48247.t1 MTR_5g093970 33.115 305 178 9 182 478 43 329 1.13e-38 144
MS.gene48247.t1 MTR_2g436600 31.348 319 184 9 172 480 45 338 1.43e-37 141
MS.gene48247.t1 MTR_2g073390 30.247 324 203 8 169 483 58 367 2.75e-37 140
MS.gene48247.t1 MTR_4g074290 31.193 327 196 9 169 483 58 367 5.72e-36 137
MS.gene48247.t1 MTR_4g051810 32.441 299 182 6 182 478 37 317 1.07e-35 135
MS.gene48247.t1 MTR_5g088280 31.229 301 188 5 179 478 36 318 1.88e-35 135
MS.gene48247.t1 MTR_5g088290 31.229 301 183 7 182 478 48 328 3.31e-34 132
MS.gene48247.t1 MTR_0009s0350 29.448 326 203 9 169 483 58 367 2.45e-32 127
MS.gene48247.t1 MTR_0009s0340 29.448 326 203 9 169 483 58 367 2.45e-32 127
MS.gene48247.t1 MTR_8g036225 27.302 315 200 6 182 486 33 328 4.09e-30 120
MS.gene48247.t1 MTR_6g025590 38.122 181 102 3 169 339 36 216 1.88e-29 116
MS.gene48247.t1 MTR_2g436070 27.957 279 157 10 4 251 56 321 8.54e-20 91.3
MS.gene48247.t1 MTR_0408s0030 41.414 99 45 3 355 451 36 123 4.39e-16 75.9
MS.gene48247.t1 MTR_4g130790 28.112 249 147 8 185 420 25 254 4.91e-16 78.6
MS.gene48247.t1 MTR_6g024200 34.375 160 84 5 320 478 137 276 2.24e-15 77.0
MS.gene48247.t1 MTR_7g102580 40.659 91 44 2 249 334 68 153 2.64e-12 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene48247.t1 AT2G45220 67.008 488 155 4 3 489 29 511 0.0 696
MS.gene48247.t1 AT5G51490 51.389 504 220 9 7 489 37 536 8.96e-178 509
MS.gene48247.t1 AT5G51500 51.093 503 223 10 7 489 41 540 7.46e-173 497
MS.gene48247.t1 AT3G47400 49.609 512 233 9 3 489 83 594 2.58e-172 498
MS.gene48247.t1 AT4G00190 53.183 487 203 9 8 489 8 474 3.44e-171 491
MS.gene48247.t1 AT1G23200 47.547 530 217 13 8 489 38 554 4.80e-153 447
MS.gene48247.t1 AT3G43270 50.547 457 194 8 63 489 71 525 7.37e-147 431
MS.gene48247.t1 AT3G49220 44.990 509 250 8 8 489 91 596 1.16e-145 430
MS.gene48247.t1 AT3G49220 44.990 509 250 8 8 489 136 641 1.11e-144 429
MS.gene48247.t1 AT3G60730 47.629 464 201 8 62 487 58 517 2.93e-144 424
MS.gene48247.t1 AT5G53370 44.031 511 253 8 8 489 79 585 6.07e-144 425
MS.gene48247.t1 AT3G14310 48.504 468 192 8 68 486 122 589 1.37e-143 424
MS.gene48247.t1 AT5G53370 44.031 511 253 8 8 489 115 621 1.64e-143 426
MS.gene48247.t1 AT3G59010 49.471 473 207 8 39 489 66 528 9.39e-143 420
MS.gene48247.t1 AT1G53830 45.660 530 240 11 4 489 62 587 7.69e-142 420
MS.gene48247.t1 AT4G02320 47.033 455 214 10 60 489 66 518 1.84e-139 411
MS.gene48247.t1 AT1G11580 49.886 439 196 7 68 485 118 553 1.87e-139 413
MS.gene48247.t1 AT1G11580 49.886 439 196 7 68 485 139 574 4.13e-139 412
MS.gene48247.t1 AT4G33220 46.121 464 208 6 66 489 62 523 3.87e-137 405
MS.gene48247.t1 AT3G05620 49.231 455 187 11 67 489 99 541 8.46e-135 400
MS.gene48247.t1 AT4G33220 56.627 332 141 2 161 489 71 402 1.18e-134 395
MS.gene48247.t1 AT2G43050 48.843 432 189 10 75 489 100 516 3.13e-129 385
MS.gene48247.t1 AT4G02330 44.220 545 233 17 8 489 35 571 1.21e-128 385
MS.gene48247.t1 AT4G02300 45.169 445 212 10 69 486 90 529 6.09e-127 380
MS.gene48247.t1 AT1G02810 42.523 555 237 14 8 489 32 577 1.12e-125 378
MS.gene48247.t1 AT2G26450 42.400 500 250 13 8 484 122 606 4.04e-125 378
MS.gene48247.t1 AT5G04960 41.683 511 255 13 4 487 68 562 3.63e-123 371
MS.gene48247.t1 AT3G10720 42.251 542 244 16 8 489 83 615 1.67e-122 371
MS.gene48247.t1 AT2G47550 42.373 531 246 13 8 489 39 558 6.48e-122 368
MS.gene48247.t1 AT3G10710 42.495 513 247 13 4 489 70 561 1.30e-120 365
MS.gene48247.t1 AT5G20860 40.661 514 271 12 2 489 33 538 1.41e-120 363
MS.gene48247.t1 AT1G53840 40.192 520 260 12 4 483 73 581 2.28e-119 362
MS.gene48247.t1 AT3G05610 41.280 453 234 10 66 489 116 565 2.75e-117 359
MS.gene48247.t1 AT2G26440 39.922 516 261 12 7 485 40 543 2.77e-117 355
MS.gene48247.t1 AT5G04970 41.328 542 249 17 8 489 88 620 2.39e-116 355
MS.gene48247.t1 AT5G49180 41.598 488 247 14 34 485 81 566 3.56e-116 353
MS.gene48247.t1 AT3G06830 39.425 487 255 11 39 489 86 568 5.19e-116 353
MS.gene48247.t1 AT5G27870 41.124 445 236 8 66 485 115 558 8.28e-114 352
MS.gene48247.t1 AT4G33230 40.577 520 257 14 4 484 95 601 1.89e-113 348
MS.gene48247.t1 AT3G10720 60.385 260 101 2 231 489 1 259 1.67e-110 328
MS.gene48247.t1 AT3G14300 39.370 508 261 12 8 479 463 959 1.30e-109 347
MS.gene48247.t1 AT4G15980 47.564 349 174 5 147 487 353 700 9.43e-107 333
MS.gene48247.t1 AT4G15980 47.851 349 173 5 147 487 363 710 2.22e-106 332
MS.gene48247.t1 AT3G27980 42.893 394 216 5 103 489 106 497 4.83e-105 322
MS.gene48247.t1 AT1G11590 43.000 400 215 5 101 489 127 524 1.17e-101 315
MS.gene48247.t1 AT4G03930 42.462 398 216 6 103 489 129 524 2.28e-101 314
MS.gene48247.t1 AT5G64640 38.878 517 271 16 3 481 92 601 6.17e-100 313
MS.gene48247.t1 AT5G09760 38.039 510 274 14 3 477 44 546 1.33e-97 305
MS.gene48247.t1 AT5G09760 38.039 510 274 14 3 477 70 572 3.05e-97 305
MS.gene48247.t1 AT5G20860 37.525 493 246 12 2 468 33 489 7.34e-97 302
MS.gene48247.t1 AT2G47030 44.540 348 173 6 160 489 243 588 1.09e-85 275
MS.gene48247.t1 AT3G62170 41.737 357 180 6 154 484 229 583 3.62e-82 266
MS.gene48247.t1 AT2G47040 43.768 345 177 6 160 489 253 595 4.09e-82 266
MS.gene48247.t1 AT1G11370 48.996 249 124 2 174 419 38 286 1.32e-80 252
MS.gene48247.t1 AT5G19730 37.864 309 165 6 183 478 87 381 1.16e-54 188
MS.gene48247.t1 AT3G17060 33.229 319 185 7 172 479 36 337 2.58e-49 172
MS.gene48247.t1 AT1G05310 33.934 333 189 8 159 478 74 388 2.33e-48 171
MS.gene48247.t1 AT2G36700 33.444 302 182 4 184 478 44 333 5.83e-48 169
MS.gene48247.t1 AT3G29090 35.106 282 161 4 182 451 7 278 6.00e-48 168
MS.gene48247.t1 AT5G07430 31.351 370 228 8 119 478 2 355 7.93e-46 164
MS.gene48247.t1 AT2G21610 32.890 301 179 7 182 478 51 332 1.12e-45 163
MS.gene48247.t1 AT2G21610 32.890 301 179 7 182 478 56 337 1.31e-45 163
MS.gene48247.t1 AT2G36710 32.372 312 189 7 182 483 91 390 2.16e-44 161
MS.gene48247.t1 AT5G55590 33.739 329 190 8 164 478 64 378 3.69e-43 157
MS.gene48247.t1 AT5G07420 32.390 318 191 7 169 478 54 355 6.17e-43 156
MS.gene48247.t1 AT5G47500 30.699 329 191 7 164 478 52 357 3.72e-42 154
MS.gene48247.t1 AT5G18990 33.441 311 177 7 182 478 29 323 6.46e-42 152
MS.gene48247.t1 AT2G19150 33.754 317 179 9 182 485 41 339 2.82e-41 151
MS.gene48247.t1 AT3G24130 31.348 319 185 8 182 484 34 334 1.17e-40 149
MS.gene48247.t1 AT5G55590 35.165 273 152 7 164 422 64 325 3.90e-40 147
MS.gene48247.t1 AT5G61680 31.646 316 190 8 173 478 59 358 1.07e-39 147
MS.gene48247.t1 AT1G44980 41.489 188 104 2 162 343 54 241 1.12e-39 144
MS.gene48247.t1 AT1G69940 29.141 326 206 7 162 478 46 355 3.13e-38 143
MS.gene48247.t1 AT5G07410 29.141 326 206 7 162 478 46 355 2.52e-37 140
MS.gene48247.t1 AT5G26810 32.692 312 183 8 182 478 34 333 3.36e-37 140
MS.gene48247.t1 AT2G47280 32.476 311 178 8 182 478 35 327 1.49e-34 132
MS.gene48247.t1 AT2G19150 34.921 252 140 7 182 422 41 279 4.91e-34 130
MS.gene48247.t1 AT3G49220 34.764 233 122 7 68 274 194 422 5.47e-33 130

Find 103 sgRNAs with CRISPR-Local

Find 142 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTACGGTTGATTTCATATT+TGG 0.122126 4.2:-77452248 MS.gene48247:CDS
TTGCAAATATGTTACAATTT+TGG 0.131621 4.2:+77452207 None:intergenic
TACAAACAATGTAACATTTA+TGG 0.134217 4.2:-77452269 MS.gene48247:CDS
GATAAGTCTTAACAGAATTT+TGG 0.160141 4.2:+77452066 None:intergenic
GCATTTCTATTCACAATTCT+AGG 0.200545 4.2:-77452118 MS.gene48247:CDS
TACCTACCTTTGTTATTCTT+TGG 0.232922 4.2:+77453423 None:intergenic
GCATTAAGTACTTTATATTA+TGG 0.242821 4.2:-77451942 MS.gene48247:CDS
CCAAAGTTTAACTTCATTCA+TGG 0.264428 4.2:+77454031 None:intergenic
TGTTGTTGGAGATGGATTTA+TGG 0.279809 4.2:-77452438 MS.gene48247:CDS
TGGCTCAAGATATCACATTT+AGG 0.292215 4.2:-77452418 MS.gene48247:CDS
ACTGTATCAAATTTCATTGC+TGG 0.309927 4.2:-77451822 MS.gene48247:CDS
ACACACAAGGTTGAGGATTA+GGG 0.312500 4.2:+77453997 None:intergenic
TTCACGTGAGTTACTACATT+TGG 0.320043 4.2:+77453845 None:intergenic
TGATTTCTTCAAACTTTCAT+TGG 0.324376 4.2:-77453922 MS.gene48247:CDS
GTGATTGTGTTGACTTTGTT+TGG 0.331970 4.2:+77452176 None:intergenic
GTAAATGGCACTTTGGTTGC+AGG 0.349440 4.2:+77451786 None:intergenic
ATCCATCTGGTTGGTTAGAA+TGG 0.351047 4.2:-77451977 MS.gene48247:CDS
AATTCTGTTAAGACTTATCT+TGG 0.359832 4.2:-77452062 MS.gene48247:CDS
TACACACAAGGTTGAGGATT+AGG 0.360450 4.2:+77453996 None:intergenic
AATTAAAGAGTAGATGTAAA+TGG 0.364262 4.2:+77451771 None:intergenic
TACTACAAACTTCATCTTCT+CGG 0.370790 4.2:-77453515 MS.gene48247:CDS
CAAGCTGTTGCATTGCGTTC+CGG 0.373938 4.2:-77452371 MS.gene48247:CDS
GACCATGGAAGCAATATTCT+AGG 0.374299 4.2:-77452037 MS.gene48247:CDS
CATGCAAACACTCTTTCATT+AGG 0.385373 4.2:-77453870 MS.gene48247:CDS
CAATGAACAAGTTGAGATAA+AGG 0.386142 4.2:-77453373 MS.gene48247:CDS
TTCTAGGGTTACCGCTGCTT+CGG 0.395543 4.2:-77452102 MS.gene48247:CDS
TAGCAATTTAGTAACATTGT+TGG 0.401894 4.2:+77453629 None:intergenic
CGCTGCTTCGGATTTGAAAC+CGG 0.416213 4.2:-77452090 MS.gene48247:CDS
GGGAACCCATCTTTGTAGCT+AGG 0.418473 4.2:+77453567 None:intergenic
GATAGTTTGATTCATCCATC+TGG 0.426673 4.2:-77451990 MS.gene48247:CDS
AACGCAATGCAACAGCTTGA+TGG 0.429138 4.2:+77452375 None:intergenic
GAATAGAAATGCCAGTGTTT+TGG 0.434967 4.2:+77452129 None:intergenic
GCAAAGAATATTATGTTGGT+TGG 0.435785 4.2:-77453351 MS.gene48247:CDS
ATTCTTTGGTATAGCATTCA+CGG 0.447221 4.2:+77453437 None:intergenic
CCATGAATGAAGTTAAACTT+TGG 0.447619 4.2:-77454031 MS.gene48247:CDS
CATTTCTATTCACAATTCTA+GGG 0.450955 4.2:-77452117 MS.gene48247:CDS
TTTGCAGCTGTTGTTGGAGA+TGG 0.451928 4.2:-77452446 MS.gene48247:intron
CACCTGGCCTGACCCATGTT+GGG 0.452533 4.2:+77453547 None:intergenic
ATGAGTTTGTGTTGGTAAGT+AGG 0.457261 4.2:+77453957 None:intergenic
TTCCCAACATGGGTCAGGCC+AGG 0.470969 4.2:-77453549 MS.gene48247:CDS
TGAGTTTGTGTTGGTAAGTA+GGG 0.473331 4.2:+77453958 None:intergenic
ATGGGTTCCCAACATGGGTC+AGG 0.478964 4.2:-77453554 MS.gene48247:CDS
AATATTATGTTGGTTGGAGA+TGG 0.480645 4.2:-77453345 MS.gene48247:CDS
AAAGGCAAAGAATATTATGT+TGG 0.483951 4.2:-77453355 MS.gene48247:CDS
CCATTTGACACGGTTTCTTG+TGG 0.485738 4.2:+77451890 None:intergenic
GAGTATATGAACACTGGTCT+TGG 0.488203 4.2:-77451918 MS.gene48247:CDS
TTGTGTTGACTTTGTTTGGA+GGG 0.490707 4.2:+77452180 None:intergenic
CAAGAACCTAGCTACAAAGA+TGG 0.493394 4.2:-77453573 MS.gene48247:CDS
ATTTCATTGCTGGAAATGCT+TGG 0.496886 4.2:-77451812 MS.gene48247:CDS
CCACAAGAAACCGTGTCAAA+TGG 0.501895 4.2:-77451890 MS.gene48247:CDS
AAGAAATACACACAAGGTTG+AGG 0.502901 4.2:+77453990 None:intergenic
GCCCTAGAATATTGCTTCCA+TGG 0.507314 4.2:+77452035 None:intergenic
TAAATTGCTAAGTAACACTT+TGG 0.507939 4.2:-77453616 MS.gene48247:CDS
ACGTAGTCGTGGCGAAAGAC+GGG 0.518740 4.2:-77453488 MS.gene48247:CDS
TGTAGTAACTTTCTATCACC+TGG 0.519105 4.2:+77453531 None:intergenic
TTTGTTTGATGAGTTTGTGT+TGG 0.521126 4.2:+77453949 None:intergenic
AGTAGATGTAAATGGCACTT+TGG 0.524094 4.2:+77451779 None:intergenic
AACGTAGTCGTGGCGAAAGA+CGG 0.524269 4.2:-77453489 MS.gene48247:CDS
ACCGGTAGCAAAAGCGTTGG+AGG 0.524747 4.2:-77453303 MS.gene48247:CDS
AAATACAGACAAGTCTGATC+CGG 0.525381 4.2:+77452352 None:intergenic
ATGTTGGTTGGAGATGGTAT+TGG 0.525732 4.2:-77453339 MS.gene48247:CDS
AAAGCTGGATTCTATGATCT+TGG 0.527056 4.2:-77453681 MS.gene48247:CDS
ACAATGTAACATTTATGGTA+CGG 0.531513 4.2:-77452264 MS.gene48247:CDS
ACAAAGATGGGTTCCCAACA+TGG 0.535204 4.2:-77453560 MS.gene48247:CDS
TCTTTCGCCACGACTACGTT+AGG 0.545212 4.2:+77453492 None:intergenic
TCACCTGGCCTGACCCATGT+TGG 0.545397 4.2:+77453546 None:intergenic
AGCTTTACATGTCTCAAGGT+TGG 0.546562 4.2:+77453698 None:intergenic
AACCTTGAGACATGTAAAGC+TGG 0.551306 4.2:-77453696 MS.gene48247:CDS
AAGAACCTAGCTACAAAGAT+GGG 0.557927 4.2:-77453572 MS.gene48247:CDS
ACCTTCCGTTCTGCCACTGT+TGG 0.562514 4.2:-77453270 MS.gene48247:intron
GTTTGATTCATCCATCTGGT+TGG 0.562831 4.2:-77451986 MS.gene48247:CDS
CTCAAGTTGATGCTCAAACA+TGG 0.563888 4.2:-77453737 MS.gene48247:CDS
ATCCAGCTTTACATGTCTCA+AGG 0.564983 4.2:+77453694 None:intergenic
CACACAAGGTTGAGGATTAG+GGG 0.565318 4.2:+77453998 None:intergenic
ATTGTGTTGACTTTGTTTGG+AGG 0.571190 4.2:+77452179 None:intergenic
GTCGTGGCGAAAGACGGGTC+AGG 0.575618 4.2:-77453483 MS.gene48247:CDS
ACCAACAGTGGCAGAACGGA+AGG 0.576581 4.2:+77453269 None:intergenic
CTCCATTCTAACCAACCAGA+TGG 0.577867 4.2:+77451975 None:intergenic
GATAACCTTGCCATTTGACA+CGG 0.589011 4.2:+77451880 None:intergenic
AGTGGCAGAACGGAAGGTTG+TGG 0.591491 4.2:+77453275 None:intergenic
ACCATGGAAGCAATATTCTA+GGG 0.592357 4.2:-77452036 MS.gene48247:CDS
ATTGGTAAAACTGTAATCAC+CGG 0.592601 4.2:-77453321 MS.gene48247:CDS
CTAATGAAAGAGTGTTTGCA+TGG 0.596121 4.2:+77453871 None:intergenic
AGACTTATCTTGGAAGACCA+TGG 0.599466 4.2:-77452052 MS.gene48247:CDS
ATCACCGGTAGCAAAAGCGT+TGG 0.601310 4.2:-77453306 MS.gene48247:CDS
TATGTTATACATGTGAAAGC+TGG 0.604818 4.2:-77453402 MS.gene48247:CDS
AGAAACCGTGTCAAATGGCA+AGG 0.607462 4.2:-77451885 MS.gene48247:CDS
TTCTCGGCCTAACGTAGTCG+TGG 0.607759 4.2:-77453499 MS.gene48247:CDS
TACCAAAGAATAACAAAGGT+AGG 0.608433 4.2:-77453425 MS.gene48247:CDS
GGTAGCAAAAGCGTTGGAGG+AGG 0.610712 4.2:-77453300 MS.gene48247:CDS
ATCACATTTAGGAACACTGC+CGG 0.612058 4.2:-77452407 MS.gene48247:CDS
GTAACATTGTTGGACATGAG+AGG 0.612493 4.2:+77453639 None:intergenic
ACAGATCCTAACCAAAACAC+TGG 0.626471 4.2:-77452140 MS.gene48247:CDS
CAAAGATGGGTTCCCAACAT+GGG 0.630453 4.2:-77453559 MS.gene48247:CDS
GCTATACCAAAGAATAACAA+AGG 0.633072 4.2:-77453429 MS.gene48247:CDS
TATGGAGAGTATATGAACAC+TGG 0.633586 4.2:-77451924 MS.gene48247:CDS
ACTTACCAACAGTGGCAGAA+CGG 0.640460 4.2:+77453265 None:intergenic
GTTCAAGAAAGAGCACTCAA+AGG 0.643280 4.2:-77453894 MS.gene48247:CDS
GTCAACACAATCACTGCACA+AGG 0.651414 4.2:-77452167 MS.gene48247:CDS
CGGTTTCAAATCCGAAGCAG+CGG 0.662915 4.2:+77452091 None:intergenic
TCTGGTTGGTTAGAATGGAG+TGG 0.670039 4.2:-77451972 MS.gene48247:CDS
CAGTGACTACTTACCAACAG+TGG 0.706992 4.2:+77453257 None:intergenic
TTGTTCAAGAAATACACACA+AGG 0.713213 4.2:+77453984 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAATCACATTAAAAAAA+TGG + chr4.2:77453106-77453125 None:intergenic 10.0%
!! TATTAATGTTAAAAACTAAG+AGG + chr4.2:77453290-77453309 None:intergenic 15.0%
!!! TCTTGCAATTTTTTTTTTAA+AGG + chr4.2:77453028-77453047 None:intergenic 15.0%
!! AACATATATTTAGCGTTATT+TGG + chr4.2:77452804-77452823 None:intergenic 20.0%
!! GCATTAAGTACTTTATATTA+TGG - chr4.2:77453860-77453879 MS.gene48247:CDS 20.0%
!! TACAAACAATGTAACATTTA+TGG - chr4.2:77453533-77453552 MS.gene48247:CDS 20.0%
!! TTTCATGTATTTATAGAACT+GGG - chr4.2:77452959-77452978 MS.gene48247:intron 20.0%
!!! AAGATTTAATAGGATTTTGA+AGG + chr4.2:77453053-77453072 None:intergenic 20.0%
!!! ATGAATTTTTGGATTTAAGT+CGG - chr4.2:77453195-77453214 MS.gene48247:intron 20.0%
!!! TACTTTTGTATAATCGATAT+AGG - chr4.2:77452723-77452742 MS.gene48247:intron 20.0%
!!! TTGCAAATATGTTACAATTT+TGG + chr4.2:77453598-77453617 None:intergenic 20.0%
!!! TTTTATAGATGTAGTTTTGA+AGG - chr4.2:77453470-77453489 MS.gene48247:CDS 20.0%
! AAAGGCAAAGAATATTATGT+TGG - chr4.2:77452447-77452466 MS.gene48247:intron 25.0%
! AATTCTGTTAAGACTTATCT+TGG - chr4.2:77453740-77453759 MS.gene48247:CDS 25.0%
! ACAATGTAACATTTATGGTA+CGG - chr4.2:77453538-77453557 MS.gene48247:CDS 25.0%
! CATTTCTATTCACAATTCTA+GGG - chr4.2:77453685-77453704 MS.gene48247:CDS 25.0%
! CTTTCATGTATTTATAGAAC+TGG - chr4.2:77452958-77452977 MS.gene48247:intron 25.0%
! TAAATTGCTAAGTAACACTT+TGG - chr4.2:77452186-77452205 MS.gene48247:CDS 25.0%
! TAGCAATTTAGTAACATTGT+TGG + chr4.2:77452176-77452195 None:intergenic 25.0%
! TGATTTCTTCAAACTTTCAT+TGG - chr4.2:77451880-77451899 MS.gene48247:CDS 25.0%
! TGCTTATTATCAAGCTAAAT+CGG - chr4.2:77452617-77452636 MS.gene48247:intron 25.0%
!! AATCGGGATTAATTTAACTT+CGG - chr4.2:77452634-77452653 MS.gene48247:intron 25.0%
!! ATTAGAATTTTGCCTGTTTT+AGG - chr4.2:77453124-77453143 MS.gene48247:intron 25.0%
!! GATAAGTCTTAACAGAATTT+TGG + chr4.2:77453739-77453758 None:intergenic 25.0%
!! GTCACAAAATCATGAATTTT+TGG - chr4.2:77453184-77453203 MS.gene48247:intron 25.0%
!!! AACACTTTGTTTTAGTATCT+AGG + chr4.2:77452045-77452064 None:intergenic 25.0%
!!! ACACTTTGTTTTAGTATCTA+GGG + chr4.2:77452044-77452063 None:intergenic 25.0%
!!! TATTTAGCGTTATTTGGTTT+TGG + chr4.2:77452798-77452817 None:intergenic 25.0%
!!! TGAATTTTTGGATTTAAGTC+GGG - chr4.2:77453196-77453215 MS.gene48247:intron 25.0%
ACTGTATCAAATTTCATTGC+TGG - chr4.2:77453980-77453999 MS.gene48247:CDS 30.0%
ATTCTTTGGTATAGCATTCA+CGG + chr4.2:77452368-77452387 None:intergenic 30.0%
ATTGGTAAAACTGTAATCAC+CGG - chr4.2:77452481-77452500 MS.gene48247:intron 30.0%
GCAAAATCGCAAGATTTAAT+AGG + chr4.2:77453063-77453082 None:intergenic 30.0%
GCAAAGAATATTATGTTGGT+TGG - chr4.2:77452451-77452470 MS.gene48247:intron 30.0%
GCATTTCTATTCACAATTCT+AGG - chr4.2:77453684-77453703 MS.gene48247:CDS 30.0%
GCTATACCAAAGAATAACAA+AGG - chr4.2:77452373-77452392 MS.gene48247:CDS 30.0%
GCTTATTATCAAGCTAAATC+GGG - chr4.2:77452618-77452637 MS.gene48247:intron 30.0%
TAAACAACCTAGAGAAAACT+TGG - chr4.2:77452934-77452953 MS.gene48247:intron 30.0%
TACCAAAGAATAACAAAGGT+AGG - chr4.2:77452377-77452396 MS.gene48247:CDS 30.0%
TACTACAAACTTCATCTTCT+CGG - chr4.2:77452287-77452306 MS.gene48247:CDS 30.0%
TATGTTATACATGTGAAAGC+TGG - chr4.2:77452400-77452419 MS.gene48247:CDS 30.0%
TTGTTCAAGAAATACACACA+AGG + chr4.2:77451821-77451840 None:intergenic 30.0%
TTTGTTTGATGAGTTTGTGT+TGG + chr4.2:77451856-77451875 None:intergenic 30.0%
! AATATTATGTTGGTTGGAGA+TGG - chr4.2:77452457-77452476 MS.gene48247:intron 30.0%
! ATCGGGATTAATTTAACTTC+GGG - chr4.2:77452635-77452654 MS.gene48247:intron 30.0%
! CAATGAACAAGTTGAGATAA+AGG - chr4.2:77452429-77452448 MS.gene48247:CDS 30.0%
! CAATGGATTTGAATTGTTGA+AGG - chr4.2:77453153-77453172 MS.gene48247:intron 30.0%
! TTAAATCTTGCGATTTTGCA+TGG - chr4.2:77453064-77453083 MS.gene48247:intron 30.0%
!! TACCTACCTTTGTTATTCTT+TGG + chr4.2:77452382-77452401 None:intergenic 30.0%
AAAGCTGGATTCTATGATCT+TGG - chr4.2:77452121-77452140 MS.gene48247:CDS 35.0%
AAATACAGACAAGTCTGATC+CGG + chr4.2:77453453-77453472 None:intergenic 35.0%
AAGAAATACACACAAGGTTG+AGG + chr4.2:77451815-77451834 None:intergenic 35.0%
AAGAACCTAGCTACAAAGAT+GGG - chr4.2:77452230-77452249 MS.gene48247:CDS 35.0%
ACCATGGAAGCAATATTCTA+GGG - chr4.2:77453766-77453785 MS.gene48247:CDS 35.0%
AGTAGATGTAAATGGCACTT+TGG + chr4.2:77454026-77454045 None:intergenic 35.0%
ATTTCATTGCTGGAAATGCT+TGG - chr4.2:77453990-77454009 MS.gene48247:CDS 35.0%
CATGCAAACACTCTTTCATT+AGG - chr4.2:77451932-77451951 MS.gene48247:CDS 35.0%
GATAGTTTGATTCATCCATC+TGG - chr4.2:77453812-77453831 MS.gene48247:CDS 35.0%
TATTTATAGAACTGGGTCAC+AGG - chr4.2:77452966-77452985 MS.gene48247:intron 35.0%
TGGCTCAAGATATCACATTT+AGG - chr4.2:77453384-77453403 MS.gene48247:CDS 35.0%
TGTAGTAACTTTCTATCACC+TGG + chr4.2:77452274-77452293 None:intergenic 35.0%
TTCACGTGAGTTACTACATT+TGG + chr4.2:77451960-77451979 None:intergenic 35.0%
! AATTTAACTTCGGGTCGAAT+GGG - chr4.2:77452644-77452663 MS.gene48247:intron 35.0%
! ATGAGTTTGTGTTGGTAAGT+AGG + chr4.2:77451848-77451867 None:intergenic 35.0%
! GAATAGAAATGCCAGTGTTT+TGG + chr4.2:77453676-77453695 None:intergenic 35.0%
! GGTACGGTTGATTTCATATT+TGG - chr4.2:77453554-77453573 MS.gene48247:CDS 35.0%
! GTCTTAACAGAATTTTGGAC+CGG + chr4.2:77453734-77453753 None:intergenic 35.0%
! TAATTTAACTTCGGGTCGAA+TGG - chr4.2:77452643-77452662 MS.gene48247:intron 35.0%
! TATGGAGAGTATATGAACAC+TGG - chr4.2:77453878-77453897 MS.gene48247:CDS 35.0%
! TGAAAGACCAAGTTTTCTCT+AGG + chr4.2:77452944-77452963 None:intergenic 35.0%
! TGAGTTTGTGTTGGTAAGTA+GGG + chr4.2:77451847-77451866 None:intergenic 35.0%
! TGTTGTTGGAGATGGATTTA+TGG - chr4.2:77453364-77453383 MS.gene48247:CDS 35.0%
!! ATTGTGTTGACTTTGTTTGG+AGG + chr4.2:77453626-77453645 None:intergenic 35.0%
!! CTAATGAAAGAGTGTTTGCA+TGG + chr4.2:77451934-77451953 None:intergenic 35.0%
!! GTGATTGTGTTGACTTTGTT+TGG + chr4.2:77453629-77453648 None:intergenic 35.0%
!! TTGTGTTGACTTTGTTTGGA+GGG + chr4.2:77453625-77453644 None:intergenic 35.0%
AACCTTGAGACATGTAAAGC+TGG - chr4.2:77452106-77452125 MS.gene48247:CDS 40.0%
ACACACAAGGTTGAGGATTA+GGG + chr4.2:77451808-77451827 None:intergenic 40.0%
ACAGATCCTAACCAAAACAC+TGG - chr4.2:77453662-77453681 MS.gene48247:CDS 40.0%
ACTCACGTGAAAAATCAGCT+TGG - chr4.2:77451969-77451988 MS.gene48247:CDS 40.0%
AGACTTATCTTGGAAGACCA+TGG - chr4.2:77453750-77453769 MS.gene48247:CDS 40.0%
AGCTTTACATGTCTCAAGGT+TGG + chr4.2:77452107-77452126 None:intergenic 40.0%
ATCACATTTAGGAACACTGC+CGG - chr4.2:77453395-77453414 MS.gene48247:CDS 40.0%
ATCCAGCTTTACATGTCTCA+AGG + chr4.2:77452111-77452130 None:intergenic 40.0%
CAAGAACCTAGCTACAAAGA+TGG - chr4.2:77452229-77452248 MS.gene48247:CDS 40.0%
CCATTGCAGAATCCTAAAAC+AGG + chr4.2:77453139-77453158 None:intergenic 40.0%
CTCACGTGAAAAATCAGCTT+GGG - chr4.2:77451970-77451989 MS.gene48247:CDS 40.0%
GACCATGGAAGCAATATTCT+AGG - chr4.2:77453765-77453784 MS.gene48247:CDS 40.0%
GTAACATTGTTGGACATGAG+AGG + chr4.2:77452166-77452185 None:intergenic 40.0%
GTTTGATTCATCCATCTGGT+TGG - chr4.2:77453816-77453835 MS.gene48247:CDS 40.0%
TACACACAAGGTTGAGGATT+AGG + chr4.2:77451809-77451828 None:intergenic 40.0%
! ATCCATCTGGTTGGTTAGAA+TGG - chr4.2:77453825-77453844 MS.gene48247:CDS 40.0%
! ATGTTGGTTGGAGATGGTAT+TGG - chr4.2:77452463-77452482 MS.gene48247:intron 40.0%
! GAGTATATGAACACTGGTCT+TGG - chr4.2:77453884-77453903 MS.gene48247:CDS 40.0%
! GATAACCTTGCCATTTGACA+CGG + chr4.2:77453925-77453944 None:intergenic 40.0%
! TAATCCGTCGATTGACGAAT+CGG - chr4.2:77452991-77453010 MS.gene48247:intron 40.0%
! TGTCTTTTTGCAGCTGTTGT+TGG - chr4.2:77453350-77453369 MS.gene48247:CDS 40.0%
!! CTCAAGTTGATGCTCAAACA+TGG - chr4.2:77452065-77452084 MS.gene48247:CDS 40.0%
!! GTTCAAGAAAGAGCACTCAA+AGG - chr4.2:77451908-77451927 MS.gene48247:CDS 40.0%
!!! AGCTTGATGGTTTTTTGCAC+CGG + chr4.2:77453417-77453436 None:intergenic 40.0%
!!! CCTGTTTTAGGATTCTGCAA+TGG - chr4.2:77453136-77453155 MS.gene48247:intron 40.0%
!!! CTAGGGTTTTGTTGAGCTTA+AGG + chr4.2:77452027-77452046 None:intergenic 40.0%
!!! GAAATGCCAGTGTTTTGGTT+AGG + chr4.2:77453671-77453690 None:intergenic 40.0%
!!! GGTTTTGTTGAGCTTAAGGA+TGG + chr4.2:77452023-77452042 None:intergenic 40.0%
AACGCAATGCAACAGCTTGA+TGG + chr4.2:77453430-77453449 None:intergenic 45.0%
ACTTACCAACAGTGGCAGAA+CGG + chr4.2:77452540-77452559 None:intergenic 45.0%
AGAAACCGTGTCAAATGGCA+AGG - chr4.2:77453917-77453936 MS.gene48247:CDS 45.0%
CACACAAGGTTGAGGATTAG+GGG + chr4.2:77451807-77451826 None:intergenic 45.0%
CAGTGACTACTTACCAACAG+TGG + chr4.2:77452548-77452567 None:intergenic 45.0%
CCACAAGAAACCGTGTCAAA+TGG - chr4.2:77453912-77453931 MS.gene48247:CDS 45.0%
CCATTTGACACGGTTTCTTG+TGG + chr4.2:77453915-77453934 None:intergenic 45.0%
CTCCATTCTAACCAACCAGA+TGG + chr4.2:77453830-77453849 None:intergenic 45.0%
GCCCTAGAATATTGCTTCCA+TGG + chr4.2:77453770-77453789 None:intergenic 45.0%
GTAAATGGCACTTTGGTTGC+AGG + chr4.2:77454019-77454038 None:intergenic 45.0%
GTCAACACAATCACTGCACA+AGG - chr4.2:77453635-77453654 MS.gene48247:CDS 45.0%
TTTGCAGCTGTTGTTGGAGA+TGG - chr4.2:77453356-77453375 MS.gene48247:CDS 45.0%
! AATCCGTCGATTGACGAATC+GGG - chr4.2:77452992-77453011 MS.gene48247:intron 45.0%
! ACAAAGATGGGTTCCCAACA+TGG - chr4.2:77452242-77452261 MS.gene48247:CDS 45.0%
! CAAAGATGGGTTCCCAACAT+GGG - chr4.2:77452243-77452262 MS.gene48247:CDS 45.0%
! TCTGGTTGGTTAGAATGGAG+TGG - chr4.2:77453830-77453849 MS.gene48247:CDS 45.0%
AACGTAGTCGTGGCGAAAGA+CGG - chr4.2:77452313-77452332 MS.gene48247:CDS 50.0%
CAAGCTGTTGCATTGCGTTC+CGG - chr4.2:77453431-77453450 MS.gene48247:CDS 50.0%
CGGTTTCAAATCCGAAGCAG+CGG + chr4.2:77453714-77453733 None:intergenic 50.0%
GGGAACCCATCTTTGTAGCT+AGG + chr4.2:77452238-77452257 None:intergenic 50.0%
TCTTTCGCCACGACTACGTT+AGG + chr4.2:77452313-77452332 None:intergenic 50.0%
! ATCACCGGTAGCAAAAGCGT+TGG - chr4.2:77452496-77452515 MS.gene48247:intron 50.0%
! CGCTGCTTCGGATTTGAAAC+CGG - chr4.2:77453712-77453731 MS.gene48247:CDS 50.0%
! TCCTCCAACGCTTTTGCTAC+CGG + chr4.2:77452503-77452522 None:intergenic 50.0%
! TGACCCGATTCGTCAATCGA+CGG + chr4.2:77452998-77453017 None:intergenic 50.0%
!! TTCTAGGGTTACCGCTGCTT+CGG - chr4.2:77453700-77453719 MS.gene48247:CDS 50.0%
ACCAACAGTGGCAGAACGGA+AGG + chr4.2:77452536-77452555 None:intergenic 55.0%
ACCTTCCGTTCTGCCACTGT+TGG - chr4.2:77452532-77452551 MS.gene48247:intron 55.0%
ACGTAGTCGTGGCGAAAGAC+GGG - chr4.2:77452314-77452333 MS.gene48247:CDS 55.0%
AGTGGCAGAACGGAAGGTTG+TGG + chr4.2:77452530-77452549 None:intergenic 55.0%
ATGGGTTCCCAACATGGGTC+AGG - chr4.2:77452248-77452267 MS.gene48247:CDS 55.0%
TTCTCGGCCTAACGTAGTCG+TGG - chr4.2:77452303-77452322 MS.gene48247:CDS 55.0%
!! ACCGGTAGCAAAAGCGTTGG+AGG - chr4.2:77452499-77452518 MS.gene48247:intron 55.0%
!! GGTAGCAAAAGCGTTGGAGG+AGG - chr4.2:77452502-77452521 MS.gene48247:intron 55.0%
CACCTGGCCTGACCCATGTT+GGG + chr4.2:77452258-77452277 None:intergenic 60.0%
TCACCTGGCCTGACCCATGT+TGG + chr4.2:77452259-77452278 None:intergenic 60.0%
TTCCCAACATGGGTCAGGCC+AGG - chr4.2:77452253-77452272 MS.gene48247:CDS 60.0%
GTCGTGGCGAAAGACGGGTC+AGG - chr4.2:77452319-77452338 MS.gene48247:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 77451773 77454051 77451773 ID=MS.gene48247
chr4.2 mRNA 77451773 77454051 77451773 ID=MS.gene48247.t1;Parent=MS.gene48247
chr4.2 exon 77453271 77454051 77453271 ID=MS.gene48247.t1.exon1;Parent=MS.gene48247.t1
chr4.2 CDS 77453271 77454051 77453271 ID=cds.MS.gene48247.t1;Parent=MS.gene48247.t1
chr4.2 exon 77451773 77452461 77451773 ID=MS.gene48247.t1.exon2;Parent=MS.gene48247.t1
chr4.2 CDS 77451773 77452461 77451773 ID=cds.MS.gene48247.t1;Parent=MS.gene48247.t1
Gene Sequence

>MS.gene48247

ATGAATGAAGTTAAACTTTGGTGTAACAAAACCCCTAATCCTCAACCTTGTGTGTATTTCTTGAACAATAACCCTACTTACCAACACAAACTCATCAAACAAAAAGTTGATTTCTTCAAACTTTCATTGGATCTTGTTCAAGAAAGAGCACTCAAAGGCCATGCAAACACTCTTTCATTAGGCTCCAAATGTAGTAACTCACGTGAAAAATCAGCTTGGGCTGATTGTGTTGAGCTATATGAACAAACCATCCTTAAGCTCAACAAAACCCTAGATACTAAAACAAAGTGTTCTCAAGTTGATGCTCAAACATGGCTAAGCACAGCTCTCACCAACCTTGAGACATGTAAAGCTGGATTCTATGATCTTGGTGTTCAAGATTATATTCTTCCTCTCATGTCCAACAATGTTACTAAATTGCTAAGTAACACTTTGGCTCTTAACAAAGTTCAATATCAAGAACCTAGCTACAAAGATGGGTTCCCAACATGGGTCAGGCCAGGTGATAGAAAGTTACTACAAACTTCATCTTCTCGGCCTAACGTAGTCGTGGCGAAAGACGGGTCAGGAAAGTACACAACTGTTAGTGCAGCCGTGAATGCTATACCAAAGAATAACAAAGGTAGGTATGTTATACATGTGAAAGCTGGTGTCTACAATGAACAAGTTGAGATAAAGGCAAAGAATATTATGTTGGTTGGAGATGGTATTGGTAAAACTGTAATCACCGGTAGCAAAAGCGTTGGAGGAGGAACCACAACCTTCCGTTCTGCCACTGTTGGTAAGTAGTCACTGCTAAATTTTTTTTTTTATTGATACAAGTTTGAGATAAAATATGAAATGTTGCTTATTATCAAGCTAAATCGGGATTAATTTAACTTCGGGTCGAATGGGACAAGCAAAATCAAAAATCGTAAAACTGCAATCAAAATCGAAATATTTTACTCAACTACTTTTGTATAATCGATATAGGATAGCAAAATCAAAAATCGTAAACTCGCAATCAAAATCGATAGATTTTACCAAAACCAAATAACGCTAAATATATGTTATAAATTGTTTATATTCGTCAATGACTCAAAGTTTAAAGTCTACAAAATACTTATACATAAATGACTCAAAACTTAGATGTCAAATATTTTTTAGTAGTCATAATAACGCTAAACAACCTAGAGAAAACTTGGTCTTTCATGTATTTATAGAACTGGGTCACAGGTATAATCCGTCGATTGACGAATCGGGTCAAAATTGACCTTTAAAAAAAAAATTGCAAGAATCCTTCAAAATCCTATTAAATCTTGCGATTTTGCATGGATTTCAAGATTTTGTTCCATTTTTTTAATGTGATTTTATTAGAATTTTGCCTGTTTTAGGATTCTGCAATGGATTTGAATTGTTGAAGGTGAATAAAGTCACAAAATCATGAATTTTTGGATTTAAGTCGGGATTTGACAACAATGCTTTTTATGTCTTTAATTGTTATATATGTGTGTGTATCTAGATTAAAACATTCTCCTCTTAGTTTTTAACATTAATAGAATACACTCAATGACTAGAAATTTAACGAATATATATTATGTCTTTTTGCAGCTGTTGTTGGAGATGGATTTATGGCTCAAGATATCACATTTAGGAACACTGCCGGTGCAAAAAACCATCAAGCTGTTGCATTGCGTTCCGGATCAGACTTGTCTGTATTTTATAGATGTAGTTTTGAAGGTTTTCAAGACACATTATATGTGCATTCAGATAGACAATTCTACAAACAATGTAACATTTATGGTACGGTTGATTTCATATTTGGTAATGCTGCTGCAGTGTTCCAAAATTGTAACATATTTGCAAGAAACCCTCCAAACAAAGTCAACACAATCACTGCACAAGGTAGAACAGATCCTAACCAAAACACTGGCATTTCTATTCACAATTCTAGGGTTACCGCTGCTTCGGATTTGAAACCGGTCCAAAATTCTGTTAAGACTTATCTTGGAAGACCATGGAAGCAATATTCTAGGGCAGTTTTTATGAAGACTTCTCTTGATAGTTTGATTCATCCATCTGGTTGGTTAGAATGGAGTGGTAATTTTGCATTAAGTACTTTATATTATGGAGAGTATATGAACACTGGTCTTGGTTCTTCCACAAGAAACCGTGTCAAATGGCAAGGTTATCATGTGATAACAAGTGCTTCTGAAGCATCAAAGTTCACTGTATCAAATTTCATTGCTGGAAATGCTTGGTTACCTGCAACCAAAGTGCCATTTACATCTACTCTTTAA
Protein sequence

>MS.gene48247.t1

MNEVKLWCNKTPNPQPCVYFLNNNPTYQHKLIKQKVDFFKLSLDLVQERALKGHANTLSLGSKCSNSREKSAWADCVELYEQTILKLNKTLDTKTKCSQVDAQTWLSTALTNLETCKAGFYDLGVQDYILPLMSNNVTKLLSNTLALNKVQYQEPSYKDGFPTWVRPGDRKLLQTSSSRPNVVVAKDGSGKYTTVSAAVNAIPKNNKGRYVIHVKAGVYNEQVEIKAKNIMLVGDGIGKTVITGSKSVGGGTTTFRSATVAVVGDGFMAQDITFRNTAGAKNHQAVALRSGSDLSVFYRCSFEGFQDTLYVHSDRQFYKQCNIYGTVDFIFGNAAAVFQNCNIFARNPPNKVNTITAQGRTDPNQNTGISIHNSRVTAASDLKPVQNSVKTYLGRPWKQYSRAVFMKTSLDSLIHPSGWLEWSGNFALSTLYYGEYMNTGLGSSTRNRVKWQGYHVITSASEASKFTVSNFIAGNAWLPATKVPFTSTL