Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene011050.t1 | XP_024625350.1 | 98.5 | 133 | 2 | 0 | 1 | 133 | 1 | 133 | 2.10E-66 | 261.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene011050.t1 | P49211 | 88.0 | 133 | 16 | 0 | 1 | 133 | 1 | 133 | 2.7e-62 | 239.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene011050.t1 | I3SPQ1 | 98.5 | 133 | 2 | 0 | 1 | 133 | 1 | 133 | 1.5e-66 | 261.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene08771 | MS.gene011050 | PPI |
| MS.gene027346 | MS.gene011050 | PPI |
| MS.gene023035 | MS.gene011050 | PPI |
| MS.gene95251 | MS.gene011050 | PPI |
| MS.gene016666 | MS.gene011050 | PPI |
| MS.gene45749 | MS.gene011050 | PPI |
| MS.gene056170 | MS.gene011050 | PPI |
| MS.gene051274 | MS.gene011050 | PPI |
| MS.gene011050 | MS.gene051274 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene011050.t1 | MTR_7g053180 | 98.496 | 133 | 2 | 0 | 1 | 133 | 1 | 133 | 1.48e-95 | 271 |
| MS.gene011050.t1 | MTR_7g007920 | 96.992 | 133 | 4 | 0 | 1 | 133 | 1 | 133 | 7.59e-94 | 266 |
| MS.gene011050.t1 | MTR_8g085740 | 90.977 | 133 | 12 | 0 | 1 | 133 | 1 | 133 | 3.35e-87 | 250 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene011050.t1 | AT4G18100 | 87.970 | 133 | 16 | 0 | 1 | 133 | 1 | 133 | 2.64e-87 | 250 |
| MS.gene011050.t1 | AT5G46430 | 85.714 | 133 | 19 | 0 | 1 | 133 | 1 | 133 | 2.37e-86 | 248 |
| MS.gene011050.t1 | AT5G46430 | 85.714 | 133 | 19 | 0 | 1 | 133 | 1 | 133 | 2.37e-86 | 248 |
Find 32 sgRNAs with CRISPR-Local
Find 58 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACGCTTCTTCACAATCTTCT+TGG | 0.281194 | 7.1:+49070271 | None:intergenic |
| CGTTGAACGTGCGGCACAAT+TGG | 0.294789 | 7.1:-49069265 | MS.gene011050:CDS |
| ACCCGCCACTATTTGCCTAA+TGG | 0.370226 | 7.1:-49069489 | MS.gene011050:CDS |
| AGGGTATTGATTCACGAGTT+AGG | 0.379946 | 7.1:-49069569 | MS.gene011050:CDS |
| CAAAGCTGGCGCAGGCCAAA+GGG | 0.395273 | 7.1:-49069588 | MS.gene011050:CDS |
| GCGCAACCTGGCCAGCTTGT+TGG | 0.406434 | 7.1:+49069230 | None:intergenic |
| GCAAAGCTGGCGCAGGCCAA+AGG | 0.427216 | 7.1:-49069589 | MS.gene011050:CDS |
| ACAAACTTCTTGAATCCATT+AGG | 0.438510 | 7.1:+49069474 | None:intergenic |
| CTATGCAGCAAAGCTGGCGC+AGG | 0.457182 | 7.1:-49069596 | MS.gene011050:intron |
| TTGTCACCAACAAGCTGGCC+AGG | 0.462847 | 7.1:-49069236 | MS.gene011050:CDS |
| GTTGTGCTATGCAGCAAAGC+TGG | 0.468798 | 7.1:-49069602 | MS.gene011050:intron |
| TCATCAGGCCACAGAGTGAC+AGG | 0.478689 | 7.1:-49070238 | MS.gene011050:CDS |
| TGAATCCATTAGGCAAATAG+TGG | 0.478886 | 7.1:+49069484 | None:intergenic |
| TCAACAAAAGCTCCAGGTCC+TGG | 0.484927 | 7.1:+49069439 | None:intergenic |
| AGCGTGTCAAGAGGTTCATC+AGG | 0.489670 | 7.1:-49070253 | MS.gene011050:CDS |
| TTGATGCCTAACATTGGATA+CGG | 0.494285 | 7.1:-49069525 | MS.gene011050:CDS |
| ACAATCTTCTTGGTGAGCAA+TGG | 0.494679 | 7.1:+49070281 | None:intergenic |
| ACTCGTGAATCAATACCCTT+TGG | 0.495359 | 7.1:+49069573 | None:intergenic |
| GTTTGTCGTGCACAATGTCC+AGG | 0.506762 | 7.1:-49069457 | MS.gene011050:CDS |
| TCTGAGCCGTATCCAATGTT+AGG | 0.508029 | 7.1:+49069519 | None:intergenic |
| CGTGCACAATGTCCAGGACC+TGG | 0.538275 | 7.1:-49069451 | MS.gene011050:CDS |
| GTCTTCTTGACTGCGCAACC+TGG | 0.545129 | 7.1:+49069218 | None:intergenic |
| TCCATTAGGCAAATAGTGGC+GGG | 0.556875 | 7.1:+49069488 | None:intergenic |
| GTGACTTTGATGCCTAACAT+TGG | 0.577268 | 7.1:-49069531 | MS.gene011050:CDS |
| TGTGCATCAACAAAAGCTCC+AGG | 0.581714 | 7.1:+49069433 | None:intergenic |
| ATCCATTAGGCAAATAGTGG+CGG | 0.593712 | 7.1:+49069487 | None:intergenic |
| CGAGTTAGGAGAAAATTCAA+AGG | 0.594861 | 7.1:-49069555 | MS.gene011050:CDS |
| TGACAGGAAAATATGTGTGA+AGG | 0.603768 | 7.1:-49070222 | MS.gene011050:intron |
| AAAGGAGATCGTTGAACGTG+CGG | 0.614630 | 7.1:-49069274 | MS.gene011050:CDS |
| TGTTGTTGTCACCAACAAGC+TGG | 0.626162 | 7.1:-49069241 | MS.gene011050:CDS |
| TTGTGAAGAAGCGTGTCAAG+AGG | 0.636300 | 7.1:-49070262 | MS.gene011050:CDS |
| CTTCTTGGTGAGCAATGGAA+CGG | 0.637090 | 7.1:+49070286 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGATTAAATGTTATAATTTC+AGG | - | chr7.1:49069509-49069528 | MS.gene011050:CDS | 15.0% |
| !! | AGTAAAAAATAATAGATAAC+AGG | + | chr7.1:49069793-49069812 | None:intergenic | 15.0% |
| !! | ATACATAACAACAAATAAAA+TGG | + | chr7.1:49069817-49069836 | None:intergenic | 15.0% |
| !! | GATTAAATGTTATAATTTCA+GGG | - | chr7.1:49069510-49069529 | MS.gene011050:CDS | 15.0% |
| !! | TAAATGAGTGTAATAATAAA+TGG | + | chr7.1:49069630-49069649 | None:intergenic | 15.0% |
| !! | AATAGATAAGCTTTCAAAAA+TGG | - | chr7.1:49069311-49069330 | MS.gene011050:CDS | 20.0% |
| !!! | CATTTTCAGCAAAAATAATT+AGG | + | chr7.1:49069572-49069591 | None:intergenic | 20.0% |
| ! | ATCAAATGATTTAGTGTGTT+TGG | - | chr7.1:49069380-49069399 | MS.gene011050:intron | 25.0% |
| !!! | ATTTGTTGTTATGTATATGC+TGG | - | chr7.1:49069821-49069840 | MS.gene011050:intron | 25.0% |
| ACAAACTTCTTGAATCCATT+AGG | + | chr7.1:49070031-49070050 | None:intergenic | 30.0% | |
| AGGTACATGCGTGTAATAAA+TGG | + | chr7.1:49069552-49069571 | None:intergenic | 35.0% | |
| CGAGTTAGGAGAAAATTCAA+AGG | - | chr7.1:49069947-49069966 | MS.gene011050:intron | 35.0% | |
| TAGTGTGTTTGGAAAACTGT+TGG | - | chr7.1:49069391-49069410 | MS.gene011050:intron | 35.0% | |
| TGAATCCATTAGGCAAATAG+TGG | + | chr7.1:49070021-49070040 | None:intergenic | 35.0% | |
| TGACAGGAAAATATGTGTGA+AGG | - | chr7.1:49069280-49069299 | MS.gene011050:CDS | 35.0% | |
| TGCATAGCACAACAGTAATA+AGG | + | chr7.1:49069893-49069912 | None:intergenic | 35.0% | |
| TGTGTCAACCAGAAAAAGAA+AGG | - | chr7.1:49070210-49070229 | MS.gene011050:intron | 35.0% | |
| TGTTTGGAAAACTGTTGGAT+AGG | - | chr7.1:49069396-49069415 | MS.gene011050:intron | 35.0% | |
| TTGATGCCTAACATTGGATA+CGG | - | chr7.1:49069977-49069996 | MS.gene011050:intron | 35.0% | |
| ! | AACGATCTCCTTTCTTTTTC+TGG | + | chr7.1:49070221-49070240 | None:intergenic | 35.0% |
| AAAGCTCAAACATACCACGA+AGG | + | chr7.1:49069422-49069441 | None:intergenic | 40.0% | |
| ACAATCTTCTTGGTGAGCAA+TGG | + | chr7.1:49069224-49069243 | None:intergenic | 40.0% | |
| ACGCTTCTTCACAATCTTCT+TGG | + | chr7.1:49069234-49069253 | None:intergenic | 40.0% | |
| ACTCGTGAATCAATACCCTT+TGG | + | chr7.1:49069932-49069951 | None:intergenic | 40.0% | |
| AGTTACGAAGAGACAATGAG+AGG | + | chr7.1:49070132-49070151 | None:intergenic | 40.0% | |
| ATCCATTAGGCAAATAGTGG+CGG | + | chr7.1:49070018-49070037 | None:intergenic | 40.0% | |
| CATTTACATGAACAACCCAC+AGG | - | chr7.1:49070161-49070180 | MS.gene011050:intron | 40.0% | |
| GTGACTTTGATGCCTAACAT+TGG | - | chr7.1:49069971-49069990 | MS.gene011050:intron | 40.0% | |
| TAACAGGTATCAGTAGCAGT+AGG | + | chr7.1:49069777-49069796 | None:intergenic | 40.0% | |
| ! | AGCTTTTGTTGATGCACAAC+AGG | - | chr7.1:49070074-49070093 | MS.gene011050:intron | 40.0% |
| ! | CATATTTTCCTGTCACTCTG+TGG | + | chr7.1:49069275-49069294 | None:intergenic | 40.0% |
| !! | AGGGTATTGATTCACGAGTT+AGG | - | chr7.1:49069933-49069952 | MS.gene011050:intron | 40.0% |
| AAAGGAGATCGTTGAACGTG+CGG | - | chr7.1:49070228-49070247 | MS.gene011050:CDS | 45.0% | |
| AGGTATCAGTAGCAGTAGGT+AGG | + | chr7.1:49069773-49069792 | None:intergenic | 45.0% | |
| CTTCTTGGTGAGCAATGGAA+CGG | + | chr7.1:49069219-49069238 | None:intergenic | 45.0% | |
| GTTGATGCACAACAGGTAAC+TGG | - | chr7.1:49070081-49070100 | MS.gene011050:intron | 45.0% | |
| TCCATTAGGCAAATAGTGGC+GGG | + | chr7.1:49070017-49070036 | None:intergenic | 45.0% | |
| TCTGAGCCGTATCCAATGTT+AGG | + | chr7.1:49069986-49070005 | None:intergenic | 45.0% | |
| TGTGCATCAACAAAAGCTCC+AGG | + | chr7.1:49070072-49070091 | None:intergenic | 45.0% | |
| TGTTGTTGTCACCAACAAGC+TGG | - | chr7.1:49070261-49070280 | MS.gene011050:CDS | 45.0% | |
| ! | TTGTGAAGAAGCGTGTCAAG+AGG | - | chr7.1:49069240-49069259 | MS.gene011050:CDS | 45.0% |
| AACTGTTGGATAGGCCTTCG+TGG | - | chr7.1:49069405-49069424 | MS.gene011050:intron | 50.0% | |
| ACCCGCCACTATTTGCCTAA+TGG | - | chr7.1:49070013-49070032 | MS.gene011050:intron | 50.0% | |
| AGCGTGTCAAGAGGTTCATC+AGG | - | chr7.1:49069249-49069268 | MS.gene011050:CDS | 50.0% | |
| CTCAGCACAGTATGTCCTGT+GGG | + | chr7.1:49070179-49070198 | None:intergenic | 50.0% | |
| GTTGTGCTATGCAGCAAAGC+TGG | - | chr7.1:49069900-49069919 | MS.gene011050:intron | 50.0% | |
| GTTTGTCGTGCACAATGTCC+AGG | - | chr7.1:49070045-49070064 | MS.gene011050:intron | 50.0% | |
| TCAACAAAAGCTCCAGGTCC+TGG | + | chr7.1:49070066-49070085 | None:intergenic | 50.0% | |
| TCTCAGCACAGTATGTCCTG+TGG | + | chr7.1:49070180-49070199 | None:intergenic | 50.0% | |
| CGTTGAACGTGCGGCACAAT+TGG | - | chr7.1:49070237-49070256 | MS.gene011050:CDS | 55.0% | |
| GTCTTCTTGACTGCGCAACC+TGG | + | chr7.1:49070287-49070306 | None:intergenic | 55.0% | |
| TCATCAGGCCACAGAGTGAC+AGG | - | chr7.1:49069264-49069283 | MS.gene011050:CDS | 55.0% | |
| TTGTCACCAACAAGCTGGCC+AGG | - | chr7.1:49070266-49070285 | MS.gene011050:CDS | 55.0% | |
| CAAAGCTGGCGCAGGCCAAA+GGG | - | chr7.1:49069914-49069933 | MS.gene011050:intron | 60.0% | |
| CGTGCACAATGTCCAGGACC+TGG | - | chr7.1:49070051-49070070 | MS.gene011050:intron | 60.0% | |
| CTATGCAGCAAAGCTGGCGC+AGG | - | chr7.1:49069906-49069925 | MS.gene011050:intron | 60.0% | |
| GCAAAGCTGGCGCAGGCCAA+AGG | - | chr7.1:49069913-49069932 | MS.gene011050:intron | 65.0% | |
| GCGCAACCTGGCCAGCTTGT+TGG | + | chr7.1:49070275-49070294 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 49069212 | 49070312 | 49069212 | ID=MS.gene011050 |
| chr7.1 | mRNA | 49069212 | 49070312 | 49069212 | ID=MS.gene011050.t1;Parent=MS.gene011050 |
| chr7.1 | exon | 49070223 | 49070312 | 49070223 | ID=MS.gene011050.t1.exon1;Parent=MS.gene011050.t1 |
| chr7.1 | CDS | 49070223 | 49070312 | 49070223 | ID=cds.MS.gene011050.t1;Parent=MS.gene011050.t1 |
| chr7.1 | exon | 49069429 | 49069610 | 49069429 | ID=MS.gene011050.t1.exon2;Parent=MS.gene011050.t1 |
| chr7.1 | CDS | 49069429 | 49069610 | 49069429 | ID=cds.MS.gene011050.t1;Parent=MS.gene011050.t1 |
| chr7.1 | exon | 49069212 | 49069341 | 49069212 | ID=MS.gene011050.t1.exon3;Parent=MS.gene011050.t1 |
| chr7.1 | CDS | 49069212 | 49069341 | 49069212 | ID=cds.MS.gene011050.t1;Parent=MS.gene011050.t1 |
| Gene Sequence |
| Protein sequence |
