Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45749.t1 | XP_024625350.1 | 98.5 | 133 | 2 | 0 | 1 | 133 | 1 | 133 | 1.20E-66 | 262.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45749.t1 | P49211 | 88.0 | 133 | 16 | 0 | 1 | 133 | 1 | 133 | 1.6e-62 | 240.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45749.t1 | I3SPQ1 | 98.5 | 133 | 2 | 0 | 1 | 133 | 1 | 133 | 8.8e-67 | 262.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049386 | MS.gene45749 | 0.888047 | 8.35E-73 | -1.69E-46 |
| MS.gene059339 | MS.gene45749 | 0.879499 | 1.19E-69 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene45749 | MS.gene95251 | PPI |
| MS.gene45749 | MS.gene023035 | PPI |
| MS.gene45749 | MS.gene016666 | PPI |
| MS.gene45749 | MS.gene051274 | PPI |
| MS.gene45749 | MS.gene027346 | PPI |
| MS.gene45749 | MS.gene056170 | PPI |
| MS.gene45749 | MS.gene011050 | PPI |
| MS.gene08771 | MS.gene45749 | PPI |
| MS.gene45749 | MS.gene08771 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45749.t1 | MTR_7g053180 | 98.496 | 133 | 2 | 0 | 1 | 133 | 1 | 133 | 5.55e-96 | 272 |
| MS.gene45749.t1 | MTR_7g007920 | 96.992 | 133 | 4 | 0 | 1 | 133 | 1 | 133 | 3.33e-94 | 268 |
| MS.gene45749.t1 | MTR_8g085740 | 90.977 | 133 | 12 | 0 | 1 | 133 | 1 | 133 | 1.35e-87 | 251 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45749.t1 | AT4G18100 | 87.970 | 133 | 16 | 0 | 1 | 133 | 1 | 133 | 1.03e-87 | 251 |
| MS.gene45749.t1 | AT5G46430 | 85.714 | 133 | 19 | 0 | 1 | 133 | 1 | 133 | 1.14e-86 | 248 |
| MS.gene45749.t1 | AT5G46430 | 85.714 | 133 | 19 | 0 | 1 | 133 | 1 | 133 | 1.14e-86 | 248 |
Find 31 sgRNAs with CRISPR-Local
Find 100 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGTTGAACGTGCAGCTCAAT+TGG | 0.326038 | 8.3:-58991604 | MS.gene45749:CDS |
| TTGATGCCAAACATTGGATA+TGG | 0.326130 | 8.3:-58991866 | MS.gene45749:CDS |
| TGTGCACAATGTCCAGGATT+TGG | 0.380353 | 8.3:-58991792 | MS.gene45749:CDS |
| AGGGTATTGATTCCCGTGTT+AGG | 0.396188 | 8.3:-58991910 | MS.gene45749:CDS |
| GCGCAACCTGGCGAGCTTGT+TGG | 0.407725 | 8.3:+58991569 | None:intergenic |
| TCTGAGCCATATCCAATGTT+TGG | 0.407849 | 8.3:+58991860 | None:intergenic |
| ACAAACTTCTTGAAACCATT+AGG | 0.435341 | 8.3:+58991815 | None:intergenic |
| ACCCGCCACTATCTGCCTAA+TGG | 0.462229 | 8.3:-58991830 | MS.gene45749:CDS |
| TCATCAAAAGTTCCAAATCC+TGG | 0.470149 | 8.3:+58991780 | None:intergenic |
| TGAAACCATTAGGCAGATAG+TGG | 0.475780 | 8.3:+58991825 | None:intergenic |
| GTTTGTTGTGCACAATGTCC+AGG | 0.477032 | 8.3:-58991798 | MS.gene45749:CDS |
| GCAAAGCTGGCGTAGGCCAA+AGG | 0.477684 | 8.3:-58991930 | MS.gene45749:CDS |
| TTGTCACCAACAAGCTCGCC+AGG | 0.480042 | 8.3:-58991575 | MS.gene45749:CDS |
| ACACGGGAATCAATACCCTT+TGG | 0.483819 | 8.3:+58991914 | None:intergenic |
| CAAAGCTGGCGTAGGCCAAA+GGG | 0.488390 | 8.3:-58991929 | MS.gene45749:CDS |
| AGCGTGTCAAGAGGTTCATC+AGG | 0.489670 | 8.3:-58992967 | MS.gene45749:CDS |
| CTTTGAACTTACGCCTAACA+CGG | 0.510702 | 8.3:+58991897 | None:intergenic |
| GTTATGGTATGCAGCAAAGC+TGG | 0.513607 | 8.3:-58991943 | MS.gene45749:intron |
| CGTGTTAGGCGTAAGTTCAA+AGG | 0.520936 | 8.3:-58991896 | MS.gene45749:CDS |
| GTTACTTTGATGCCAAACAT+TGG | 0.535195 | 8.3:-58991872 | MS.gene45749:CDS |
| TCATCAGGCCTCAAAGTGAC+AGG | 0.536054 | 8.3:-58992952 | MS.gene45749:CDS |
| ACCATTAGGCAGATAGTGGC+GGG | 0.544473 | 8.3:+58991829 | None:intergenic |
| GTCTTCTTGACTGCGCAACC+TGG | 0.546687 | 8.3:+58991557 | None:intergenic |
| ATAATCTTCTTAGTTAGCAG+AGG | 0.547700 | 8.3:+58992995 | None:intergenic |
| TGTGTCAACCAGGAAAAGAA+AGG | 0.574402 | 8.3:-58991631 | MS.gene45749:CDS |
| TGACAGGAAAATATGTGTCA+AGG | 0.606818 | 8.3:-58992936 | MS.gene45749:intron |
| TTTGAACTTACGCCTAACAC+GGG | 0.609080 | 8.3:+58991898 | None:intergenic |
| AACCATTAGGCAGATAGTGG+CGG | 0.617842 | 8.3:+58991828 | None:intergenic |
| GTATGCAGCAAAGCTGGCGT+AGG | 0.627909 | 8.3:-58991937 | MS.gene45749:intron |
| TTATCAAGAAGCGTGTCAAG+AGG | 0.643611 | 8.3:-58992976 | MS.gene45749:CDS |
| TCGCACACAATGTGTCAACC+AGG | 0.659311 | 8.3:-58991641 | MS.gene45749:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AACTTATAAAAGTAATAAGT+CGG | - | chr8.3:58992043-58992062 | MS.gene45749:intron | 15.0% |
| !! | GAATGAAAGTAAAAAATAAA+AGG | + | chr8.3:58992512-58992531 | None:intergenic | 15.0% |
| !! | GTTAGGATAATAAATTAAAT+TGG | - | chr8.3:58992293-58992312 | MS.gene45749:intron | 15.0% |
| !!! | TCAAAGTTTTTATTTCAATT+AGG | + | chr8.3:58991977-58991996 | None:intergenic | 15.0% |
| !! | AATCTAGTTATAGTGTATTA+AGG | + | chr8.3:58991743-58991762 | None:intergenic | 20.0% |
| !! | ACAAAATGCAATAGAAAAAT+TGG | + | chr8.3:58992448-58992467 | None:intergenic | 20.0% |
| !! | ACTTATAAAAGTAATAAGTC+GGG | - | chr8.3:58992044-58992063 | MS.gene45749:intron | 20.0% |
| !! | AGTAAAAAATAAAAGGTAAC+AGG | + | chr8.3:58992505-58992524 | None:intergenic | 20.0% |
| !! | ATTGAAATAAAAACTTTGAG+TGG | - | chr8.3:58991978-58991997 | MS.gene45749:intron | 20.0% |
| !! | TAATTTGAAAGCAACAAATT+TGG | + | chr8.3:58992136-58992155 | None:intergenic | 20.0% |
| !!! | TATTTTACATGATGTTCATT+TGG | - | chr8.3:58991840-58991859 | MS.gene45749:CDS | 20.0% |
| !!! | TTTTAACATTGTTACTGTTA+TGG | - | chr8.3:58992596-58992615 | MS.gene45749:intron | 20.0% |
| !!! | TTTTGTTTGATTTCTGTTTT+TGG | - | chr8.3:58991806-58991825 | MS.gene45749:CDS | 20.0% |
| ! | AAATAAGCTACATTAGTATG+AGG | + | chr8.3:58992365-58992384 | None:intergenic | 25.0% |
| ! | AAATTAACGCATCAAAATAG+TGG | + | chr8.3:58992203-58992222 | None:intergenic | 25.0% |
| ! | AATAAGCTACATTAGTATGA+GGG | + | chr8.3:58992364-58992383 | None:intergenic | 25.0% |
| ! | AATTAACGCATCAAAATAGT+GGG | + | chr8.3:58992202-58992221 | None:intergenic | 25.0% |
| !! | CTTTGTTTATTTACATCTTC+AGG | - | chr8.3:58992875-58992894 | MS.gene45749:intron | 25.0% |
| !!! | CAAATTTTTTACAAGTGGAA+GGG | - | chr8.3:58991865-58991884 | MS.gene45749:CDS | 25.0% |
| !!! | TCAAATTTTTTACAAGTGGA+AGG | - | chr8.3:58991864-58991883 | MS.gene45749:CDS | 25.0% |
| !!! | TGGTTCAAATTTTTTACAAG+TGG | - | chr8.3:58991860-58991879 | MS.gene45749:CDS | 25.0% |
| !!! | TTGATGCGTTAATTTTGTTT+TGG | - | chr8.3:58992208-58992227 | MS.gene45749:intron | 25.0% |
| AAATTGTGAAGAAACTTGAG+AGG | + | chr8.3:58992106-58992125 | None:intergenic | 30.0% | |
| ACAAACTTCTTGAAACCATT+AGG | + | chr8.3:58992743-58992762 | None:intergenic | 30.0% | |
| ATAATCTTCTTAGTTAGCAG+AGG | + | chr8.3:58991563-58991582 | None:intergenic | 30.0% | |
| CAAGTTTCTTCACAATTTAG+AGG | - | chr8.3:58992108-58992127 | MS.gene45749:intron | 30.0% | |
| CATATGTAGAAATGCTTCTT+AGG | - | chr8.3:58991783-58991802 | MS.gene45749:CDS | 30.0% | |
| GACACAATACACTTTAAATC+TGG | + | chr8.3:58992403-58992422 | None:intergenic | 30.0% | |
| GCAATAGAAAAATTGGTAGA+AGG | + | chr8.3:58992441-58992460 | None:intergenic | 30.0% | |
| TGTATATACAGAACAGCAAA+TGG | + | chr8.3:58992534-58992553 | None:intergenic | 30.0% | |
| ! | AGTTTTTCTTGTAGTGAAGT+GGG | - | chr8.3:58991927-58991946 | MS.gene45749:CDS | 30.0% |
| ! | TAGAACCCAATGCATTTTAA+CGG | + | chr8.3:58992022-58992041 | None:intergenic | 30.0% |
| ! | TGTTTTGCATATTGAATCCT+CGG | - | chr8.3:58992563-58992582 | MS.gene45749:intron | 30.0% |
| !! | ATTTGCTGTTCTGTATATAC+AGG | - | chr8.3:58992533-58992552 | MS.gene45749:intron | 30.0% |
| !!! | CATGCTTTTTAAGTGTTGTT+AGG | - | chr8.3:58992276-58992295 | MS.gene45749:intron | 30.0% |
| !!! | TTTGATTTCTGTTTTTGGAG+AGG | - | chr8.3:58991811-58991830 | MS.gene45749:CDS | 30.0% |
| AAAAGGTAACAGGTATCAGT+AGG | + | chr8.3:58992495-58992514 | None:intergenic | 35.0% | |
| ACCTTGACACAATAAATCAC+TGG | + | chr8.3:58991692-58991711 | None:intergenic | 35.0% | |
| ATCTCACCATTACAATGCAT+TGG | + | chr8.3:58992071-58992090 | None:intergenic | 35.0% | |
| ATCTCACCGTTAAAATGCAT+TGG | - | chr8.3:58992013-58992032 | MS.gene45749:intron | 35.0% | |
| GTTAAAATATTAGACACCCG+AGG | + | chr8.3:58992583-58992602 | None:intergenic | 35.0% | |
| TCATCAAAAGTTCCAAATCC+TGG | + | chr8.3:58992778-58992797 | None:intergenic | 35.0% | |
| TCCAGTGATTTATTGTGTCA+AGG | - | chr8.3:58991688-58991707 | MS.gene45749:intron | 35.0% | |
| TCTCACCGTTAAAATGCATT+GGG | - | chr8.3:58992014-58992033 | MS.gene45749:intron | 35.0% | |
| TGAAGAAACTTGAGAGGTTT+AGG | + | chr8.3:58992100-58992119 | None:intergenic | 35.0% | |
| TGACAGGAAAATATGTGTCA+AGG | - | chr8.3:58991619-58991638 | MS.gene45749:CDS | 35.0% | |
| TTGATGCCAAACATTGGATA+TGG | - | chr8.3:58992689-58992708 | MS.gene45749:intron | 35.0% | |
| ! | CAGTTTTTCTTGTAGTGAAG+TGG | - | chr8.3:58991926-58991945 | MS.gene45749:CDS | 35.0% |
| ! | GTTACTTTGATGCCAAACAT+TGG | - | chr8.3:58992683-58992702 | MS.gene45749:intron | 35.0% |
| ! | GTTTTGCATATTGAATCCTC+GGG | - | chr8.3:58992564-58992583 | MS.gene45749:intron | 35.0% |
| !!! | AACTTTTGATGATGCACAAC+AGG | - | chr8.3:58992786-58992805 | MS.gene45749:intron | 35.0% |
| !!! | ATTTCTGTTTTTGGAGAGGA+TGG | - | chr8.3:58991815-58991834 | MS.gene45749:CDS | 35.0% |
| !!! | ATTTTTTACAAGTGGAAGGG+AGG | - | chr8.3:58991868-58991887 | MS.gene45749:CDS | 35.0% |
| !!! | CATATTTTCCTGTCACTTTG+AGG | + | chr8.3:58991614-58991633 | None:intergenic | 35.0% |
| AATTGGTAGAAGGACAAGAC+AGG | + | chr8.3:58992431-58992450 | None:intergenic | 40.0% | |
| AGTCAGAATTGCAGCTACTA+CGG | + | chr8.3:58992857-58992876 | None:intergenic | 40.0% | |
| ATCAGTAGGAACATCAGAGA+AGG | + | chr8.3:58992481-58992500 | None:intergenic | 40.0% | |
| ATTGGTAGAAGGACAAGACA+GGG | + | chr8.3:58992430-58992449 | None:intergenic | 40.0% | |
| CTTTGAACTTACGCCTAACA+CGG | + | chr8.3:58992661-58992680 | None:intergenic | 40.0% | |
| TCTGAGCCATATCCAATGTT+TGG | + | chr8.3:58992698-58992717 | None:intergenic | 40.0% | |
| TGAAACCATTAGGCAGATAG+TGG | + | chr8.3:58992733-58992752 | None:intergenic | 40.0% | |
| TGTGTCAACCAGGAAAAGAA+AGG | - | chr8.3:58992924-58992943 | MS.gene45749:intron | 40.0% | |
| TTTGAACTTACGCCTAACAC+GGG | + | chr8.3:58992660-58992679 | None:intergenic | 40.0% | |
| ! | AACGATCTCCTTTCTTTTCC+TGG | + | chr8.3:58992935-58992954 | None:intergenic | 40.0% |
| ! | TTATCAAGAAGCGTGTCAAG+AGG | - | chr8.3:58991579-58991598 | MS.gene45749:CDS | 40.0% |
| ACAAGTGGAAGGGAGGAAAA+AGG | - | chr8.3:58991875-58991894 | MS.gene45749:CDS | 45.0% | |
| ACACGGGAATCAATACCCTT+TGG | + | chr8.3:58992644-58992663 | None:intergenic | 45.0% | |
| CATTAGTATGAGGGCCTGTA+TGG | + | chr8.3:58992355-58992374 | None:intergenic | 45.0% | |
| CGTGTTAGGCGTAAGTTCAA+AGG | - | chr8.3:58992659-58992678 | MS.gene45749:intron | 45.0% | |
| GATGATGCACAACAGGTAAC+TGG | - | chr8.3:58992793-58992812 | MS.gene45749:intron | 45.0% | |
| GCCTGTATGGTTCAACAAAG+GGG | + | chr8.3:58992342-58992361 | None:intergenic | 45.0% | |
| GGCCTGTATGGTTCAACAAA+GGG | + | chr8.3:58992343-58992362 | None:intergenic | 45.0% | |
| GTATGGTTCAACAAAGGGGA+GGG | + | chr8.3:58992338-58992357 | None:intergenic | 45.0% | |
| GTCAGAATTGCAGCTACTAC+GGG | + | chr8.3:58992856-58992875 | None:intergenic | 45.0% | |
| GTTATGGTATGCAGCAAAGC+TGG | - | chr8.3:58992612-58992631 | MS.gene45749:intron | 45.0% | |
| TATGGTTCAACAAAGGGGAG+GGG | + | chr8.3:58992337-58992356 | None:intergenic | 45.0% | |
| TCCCCTTTGTTGAACCATAC+AGG | - | chr8.3:58992338-58992357 | MS.gene45749:intron | 45.0% | |
| TCGGGTCCAATGCATTGTAA+TGG | - | chr8.3:58992062-58992081 | MS.gene45749:intron | 45.0% | |
| TGTATGGTTCAACAAAGGGG+AGG | + | chr8.3:58992339-58992358 | None:intergenic | 45.0% | |
| TGTGCACAATGTCCAGGATT+TGG | - | chr8.3:58992763-58992782 | MS.gene45749:intron | 45.0% | |
| ! | AACCATTAGGCAGATAGTGG+CGG | + | chr8.3:58992730-58992749 | None:intergenic | 45.0% |
| !! | AGGGTATTGATTCCCGTGTT+AGG | - | chr8.3:58992645-58992664 | MS.gene45749:intron | 45.0% |
| !! | GTTTGTTGTGCACAATGTCC+AGG | - | chr8.3:58992757-58992776 | MS.gene45749:intron | 45.0% |
| AGCGTGTCAAGAGGTTCATC+AGG | - | chr8.3:58991588-58991607 | MS.gene45749:CDS | 50.0% | |
| CGTTGAACGTGCAGCTCAAT+TGG | - | chr8.3:58992951-58992970 | MS.gene45749:CDS | 50.0% | |
| GGGCCTGTATGGTTCAACAA+AGG | + | chr8.3:58992344-58992363 | None:intergenic | 50.0% | |
| TCATCAGGCCTCAAAGTGAC+AGG | - | chr8.3:58991603-58991622 | MS.gene45749:CDS | 50.0% | |
| TCGCACACAATGTGTCAACC+AGG | - | chr8.3:58992914-58992933 | MS.gene45749:intron | 50.0% | |
| TGGAAGGGAGGAAAAAGGAG+TGG | - | chr8.3:58991880-58991899 | MS.gene45749:CDS | 50.0% | |
| ! | ACCATTAGGCAGATAGTGGC+GGG | + | chr8.3:58992729-58992748 | None:intergenic | 50.0% |
| ACCCGCCACTATCTGCCTAA+TGG | - | chr8.3:58992725-58992744 | MS.gene45749:intron | 55.0% | |
| CAAAGCTGGCGTAGGCCAAA+GGG | - | chr8.3:58992626-58992645 | MS.gene45749:intron | 55.0% | |
| GTATGCAGCAAAGCTGGCGT+AGG | - | chr8.3:58992618-58992637 | MS.gene45749:intron | 55.0% | |
| GTCTTCTTGACTGCGCAACC+TGG | + | chr8.3:58993001-58993020 | None:intergenic | 55.0% | |
| GTTCAACAAAGGGGAGGGGA+GGG | + | chr8.3:58992333-58992352 | None:intergenic | 55.0% | |
| TTCAACAAAGGGGAGGGGAG+GGG | + | chr8.3:58992332-58992351 | None:intergenic | 55.0% | |
| TTGTCACCAACAAGCTCGCC+AGG | - | chr8.3:58992980-58992999 | MS.gene45749:CDS | 55.0% | |
| GCAAAGCTGGCGTAGGCCAA+AGG | - | chr8.3:58992625-58992644 | MS.gene45749:intron | 60.0% | |
| GGTTCAACAAAGGGGAGGGG+AGG | + | chr8.3:58992334-58992353 | None:intergenic | 60.0% | |
| GCGCAACCTGGCGAGCTTGT+TGG | + | chr8.3:58992989-58993008 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 58991551 | 58993026 | 58991551 | ID=MS.gene45749 |
| chr8.3 | mRNA | 58991551 | 58993026 | 58991551 | ID=MS.gene45749.t1;Parent=MS.gene45749 |
| chr8.3 | exon | 58992937 | 58993026 | 58992937 | ID=MS.gene45749.t1.exon1;Parent=MS.gene45749.t1 |
| chr8.3 | CDS | 58992937 | 58993026 | 58992937 | ID=cds.MS.gene45749.t1;Parent=MS.gene45749.t1 |
| chr8.3 | exon | 58991770 | 58991951 | 58991770 | ID=MS.gene45749.t1.exon2;Parent=MS.gene45749.t1 |
| chr8.3 | CDS | 58991770 | 58991951 | 58991770 | ID=cds.MS.gene45749.t1;Parent=MS.gene45749.t1 |
| chr8.3 | exon | 58991551 | 58991680 | 58991551 | ID=MS.gene45749.t1.exon3;Parent=MS.gene45749.t1 |
| chr8.3 | CDS | 58991551 | 58991680 | 58991551 | ID=cds.MS.gene45749.t1;Parent=MS.gene45749.t1 |
| Gene Sequence |
| Protein sequence |
