AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene056170


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene056170.t1 MTR_7g053180 98.496 133 2 0 1 133 1 133 5.55e-96 272
MS.gene056170.t1 MTR_7g007920 96.992 133 4 0 1 133 1 133 3.33e-94 268
MS.gene056170.t1 MTR_8g085740 90.977 133 12 0 1 133 1 133 1.35e-87 251
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene056170.t1 AT4G18100 87.970 133 16 0 1 133 1 133 1.03e-87 251
MS.gene056170.t1 AT5G46430 85.714 133 19 0 1 133 1 133 1.14e-86 248
MS.gene056170.t1 AT5G46430 85.714 133 19 0 1 133 1 133 1.14e-86 248

Find 31 sgRNAs with CRISPR-Local

Find 119 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACGCTTCTTGATAATCTTCT+TGG 0.286757 8.1:+65984991 None:intergenic
CGTTGAACGTGCAGCTCAAT+TGG 0.326038 8.1:-65983400 MS.gene056170:CDS
TTGATGCCAAACATTGGATA+TGG 0.326130 8.1:-65983662 MS.gene056170:CDS
TGTGCACAACGTCCAGGATT+TGG 0.342709 8.1:-65983588 MS.gene056170:CDS
AGGGTATTGATTCCCGTGTT+AGG 0.376726 8.1:-65983706 MS.gene056170:CDS
ACAAACTTCTTGAATCCATT+TGG 0.404947 8.1:+65983611 None:intergenic
GCGCAACCTGGCGAGCTTGT+TGG 0.407725 8.1:+65983365 None:intergenic
TCTGAGCCATATCCAATGTT+TGG 0.407849 8.1:+65983656 None:intergenic
TGAATCCATTTGGCAGATAG+TGG 0.417879 8.1:+65983621 None:intergenic
TCATCAAAAGTTCCAAATCC+TGG 0.470149 8.1:+65983576 None:intergenic
GCAAAGCTGGCGTAGGCCAA+AGG 0.477684 8.1:-65983726 MS.gene056170:CDS
TTGTCACCAACAAGCTCGCC+AGG 0.480042 8.1:-65983371 MS.gene056170:CDS
ACACGGGAATCAATACCCTT+TGG 0.483819 8.1:+65983710 None:intergenic
CAAAGCTGGCGTAGGCCAAA+GGG 0.488390 8.1:-65983725 MS.gene056170:CDS
AGCGTGTCAAGAGGTTCATC+AGG 0.489670 8.1:-65984973 MS.gene056170:CDS
GTTTGTTGTGCACAACGTCC+AGG 0.491285 8.1:-65983594 MS.gene056170:CDS
ACCCGCCACTATCTGCCAAA+TGG 0.504178 8.1:-65983626 MS.gene056170:CDS
GTTACAATATGCAGCAAAGC+TGG 0.510986 8.1:-65983739 MS.gene056170:intron
ACAATGTGTCAACCAGGAAA+AGG 0.530451 8.1:-65983431 MS.gene056170:CDS
GTTACTTTGATGCCAAACAT+TGG 0.535195 8.1:-65983668 MS.gene056170:CDS
TCATCAGGCCTCAAAGTGAC+AGG 0.536054 8.1:-65984958 MS.gene056170:CDS
TCCATTTGGCAGATAGTGGC+GGG 0.540014 8.1:+65983625 None:intergenic
GTCTTCTTGACTGCGCAACC+TGG 0.546687 8.1:+65983353 None:intergenic
ATAATCTTCTTGGTTAGCAG+AGG 0.563378 8.1:+65985001 None:intergenic
ATCCATTTGGCAGATAGTGG+CGG 0.591907 8.1:+65983624 None:intergenic
CGTGTTAGGAGAAAATTCAA+AGG 0.598188 8.1:-65983692 MS.gene056170:CDS
TGACAGGAAAATATGTGTCA+AGG 0.606818 8.1:-65984942 MS.gene056170:intron
ATATGCAGCAAAGCTGGCGT+AGG 0.630348 8.1:-65983733 MS.gene056170:intron
TGTGTCAACCAGGAAAAGGA+AGG 0.637655 8.1:-65983427 MS.gene056170:CDS
TTATCAAGAAGCGTGTCAAG+AGG 0.643611 8.1:-65984982 MS.gene056170:CDS
TCGCACACAATGTGTCAACC+AGG 0.659311 8.1:-65983437 MS.gene056170:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATGAGAGTAAAAAAATAAA+AGG + chr8.1:65984519-65984538 None:intergenic 15.0%
!! ATGTTAAAATATTATAAACC+TGG + chr8.1:65984591-65984610 None:intergenic 15.0%
!! TTATATATGGTAGAAATTTA+GGG - chr8.1:65983473-65983492 MS.gene056170:intron 15.0%
!!! ATAAAATTGTTGCTTATATA+TGG - chr8.1:65983460-65983479 MS.gene056170:intron 15.0%
!!! TTTTAACAAAACAAAATTGA+GGG + chr8.1:65983972-65983991 None:intergenic 15.0%
!!! TTTTTTGGTTCAATTATTTT+TGG - chr8.1:65983870-65983889 MS.gene056170:intron 15.0%
!! AATTTAATTACACATGTGTT+TGG + chr8.1:65984317-65984336 None:intergenic 20.0%
!! ACAAAATGCAATAGAAAAAT+TGG + chr8.1:65984456-65984475 None:intergenic 20.0%
!! AGTTTCACAAAACATATATA+AGG + chr8.1:65983530-65983549 None:intergenic 20.0%
!! CTTATATATGGTAGAAATTT+AGG - chr8.1:65983472-65983491 MS.gene056170:intron 20.0%
!! GTAAAAAAATAAAAGGTAAC+AGG + chr8.1:65984512-65984531 None:intergenic 20.0%
!! GTTATTATAAAAGTCTTTCA+GGG - chr8.1:65983769-65983788 MS.gene056170:intron 20.0%
!! TAATGTGTTTAGAAATATGT+TGG - chr8.1:65983673-65983692 MS.gene056170:CDS 20.0%
!! TGTTATTATAAAAGTCTTTC+AGG - chr8.1:65983768-65983787 MS.gene056170:intron 20.0%
!!! AATATAGAGCAATTTTTTGA+AGG - chr8.1:65983902-65983921 MS.gene056170:intron 20.0%
!!! ACATATTTCTAAACACATTA+AGG + chr8.1:65983673-65983692 None:intergenic 20.0%
!!! ATATAGAGCAATTTTTTGAA+GGG - chr8.1:65983903-65983922 MS.gene056170:intron 20.0%
!!! CATATTTCTAAACACATTAA+GGG + chr8.1:65983672-65983691 None:intergenic 20.0%
!!! GTTTTAACAAAACAAAATTG+AGG + chr8.1:65983973-65983992 None:intergenic 20.0%
! AAACAGTAACAAAATGAAAG+AGG + chr8.1:65984161-65984180 None:intergenic 25.0%
! AACTCTAAATTATCCTTTGT+TGG - chr8.1:65983990-65984009 MS.gene056170:intron 25.0%
! AATTGTGAAAAAAACTTGAG+AGG - chr8.1:65984043-65984062 MS.gene056170:intron 25.0%
! ATTGTGAAAAAAACTTGAGA+GGG - chr8.1:65984044-65984063 MS.gene056170:intron 25.0%
!! ATATGTTTTGTGAAACTTTC+TGG - chr8.1:65983533-65983552 MS.gene056170:intron 25.0%
!! CTTTGTTTATTTACATCTTC+AGG - chr8.1:65984881-65984900 MS.gene056170:intron 25.0%
!! TATGTTTTGTGAAACTTTCT+GGG - chr8.1:65983534-65983553 MS.gene056170:intron 25.0%
!!! AACAAAATGAAAGAGGTATT+TGG + chr8.1:65984154-65984173 None:intergenic 25.0%
!!! TATAGAGCAATTTTTTGAAG+GGG - chr8.1:65983904-65983923 MS.gene056170:intron 25.0%
!!! TTGGTTCAATTATTTTTGGT+GGG - chr8.1:65983874-65983893 MS.gene056170:intron 25.0%
!!! TTGTTTTGTGAATTTCGTTT+TGG - chr8.1:65984180-65984199 MS.gene056170:intron 25.0%
!!! TTTGGTTCAATTATTTTTGG+TGG - chr8.1:65983873-65983892 MS.gene056170:intron 25.0%
AATATCTAACTCACCAACAA+AGG + chr8.1:65984006-65984025 None:intergenic 30.0%
ACAAACTTCTTGAATCCATT+TGG + chr8.1:65984749-65984768 None:intergenic 30.0%
ATAAGGAGAAAAGCTCAAAT+AGG + chr8.1:65983513-65983532 None:intergenic 30.0%
GCAATAGAAAAATTGGTACA+AGG + chr8.1:65984449-65984468 None:intergenic 30.0%
GTGAGGATAATAAGCTAAAT+TGG - chr8.1:65984272-65984291 MS.gene056170:intron 30.0%
TACGTATTTGTAGTGTATCT+AGG + chr8.1:65983739-65983758 None:intergenic 30.0%
TTAATTGAACGCACAGATAA+AGG + chr8.1:65984087-65984106 None:intergenic 30.0%
! AGAAATGCTTTTCATTTCCA+AGG - chr8.1:65983797-65983816 MS.gene056170:intron 30.0%
! ATGTTTTGCAAATTGAATCC+AGG - chr8.1:65984570-65984589 MS.gene056170:intron 30.0%
! CTTTGAATTTTCTCCTAACA+CGG + chr8.1:65984667-65984686 None:intergenic 30.0%
! GAAATGCTTTTCATTTCCAA+GGG - chr8.1:65983798-65983817 MS.gene056170:intron 30.0%
! TTGTTGGTGAGTTAGATATT+TGG - chr8.1:65984006-65984025 MS.gene056170:intron 30.0%
! TTTGAATTTTCTCCTAACAC+GGG + chr8.1:65984666-65984685 None:intergenic 30.0%
!!! TGACTTACATGACGTTTTTT+TGG - chr8.1:65983855-65983874 MS.gene056170:intron 30.0%
!!! TGGTTCAATTATTTTTGGTG+GGG - chr8.1:65983875-65983894 MS.gene056170:intron 30.0%
AAAAGGTAACAGGAATCAGT+AGG + chr8.1:65984502-65984521 None:intergenic 35.0%
AATCAGTAGGAACATCAAAG+AGG + chr8.1:65984489-65984508 None:intergenic 35.0%
ATAAACCTTTGCCTGAAATC+TGG + chr8.1:65984405-65984424 None:intergenic 35.0%
ATAATCTTCTTGGTTAGCAG+AGG + chr8.1:65983359-65983378 None:intergenic 35.0%
ATTGGTACAAGGACAAAACA+TGG + chr8.1:65984438-65984457 None:intergenic 35.0%
CGTGTTAGGAGAAAATTCAA+AGG - chr8.1:65984665-65984684 MS.gene056170:intron 35.0%
TAACAATGTCTCCAGATTTC+AGG - chr8.1:65984391-65984410 MS.gene056170:intron 35.0%
TAGTGTATCTAGGAATCTGT+TGG + chr8.1:65983729-65983748 None:intergenic 35.0%
TCAAATAGGTCATTCGAAAC+AGG + chr8.1:65983499-65983518 None:intergenic 35.0%
TCATCAAAAGTTCCAAATCC+TGG + chr8.1:65984784-65984803 None:intergenic 35.0%
TGACAGGAAAATATGTGTCA+AGG - chr8.1:65983415-65983434 MS.gene056170:CDS 35.0%
TTGATGCCAAACATTGGATA+TGG - chr8.1:65984695-65984714 MS.gene056170:intron 35.0%
! AAACACATTAAGGGCATGTT+TGG + chr8.1:65983663-65983682 None:intergenic 35.0%
! ACGCTTCTTGATAATCTTCT+TGG + chr8.1:65983369-65983388 None:intergenic 35.0%
! GTTACTTTGATGCCAAACAT+TGG - chr8.1:65984689-65984708 MS.gene056170:intron 35.0%
!!! AACTTTTGATGATGCACAAC+AGG - chr8.1:65984792-65984811 MS.gene056170:intron 35.0%
!!! CATATTTTCCTGTCACTTTG+AGG + chr8.1:65983410-65983429 None:intergenic 35.0%
!!! TCAATTATTTTTGGTGGGGA+GGG - chr8.1:65983879-65983898 MS.gene056170:intron 35.0%
!!! TTCAATTATTTTTGGTGGGG+AGG - chr8.1:65983878-65983897 MS.gene056170:intron 35.0%
!!! TTTGGTTGGTTGTTTTTGGA+GGG - chr8.1:65983825-65983844 MS.gene056170:intron 35.0%
AAAGCTCAAGCATACCTTTG+AGG + chr8.1:65983704-65983723 None:intergenic 40.0%
AAATAAGCAACACGAGCACA+AGG + chr8.1:65984367-65984386 None:intergenic 40.0%
AATAAGCAACACGAGCACAA+GGG + chr8.1:65984366-65984385 None:intergenic 40.0%
ACAATGTGTCAACCAGGAAA+AGG - chr8.1:65984926-65984945 MS.gene056170:intron 40.0%
AGTCAGAATTGCAGCTACTA+AGG + chr8.1:65984863-65984882 None:intergenic 40.0%
ATATGTTGGAGAGACCTCAA+AGG - chr8.1:65983687-65983706 MS.gene056170:CDS 40.0%
GTCAGAATTGCAGCTACTAA+GGG + chr8.1:65984862-65984881 None:intergenic 40.0%
GTTACAATATGCAGCAAAGC+TGG - chr8.1:65984618-65984637 MS.gene056170:intron 40.0%
TCTGAGCCATATCCAATGTT+TGG + chr8.1:65984704-65984723 None:intergenic 40.0%
TGAATCCATTTGGCAGATAG+TGG + chr8.1:65984739-65984758 None:intergenic 40.0%
! TTACACATGTGTTTGGTTCG+CGG + chr8.1:65984310-65984329 None:intergenic 40.0%
! TTATCAAGAAGCGTGTCAAG+AGG - chr8.1:65983375-65983394 MS.gene056170:CDS 40.0%
! TTCATATGACAAGTGCTGTG+AGG - chr8.1:65984255-65984274 MS.gene056170:intron 40.0%
!! CGTGTTTGGTTGGTTGTTTT+TGG - chr8.1:65983821-65983840 MS.gene056170:intron 40.0%
!!! GTTTGGTTGGTTGTTTTTGG+AGG - chr8.1:65983824-65983843 MS.gene056170:intron 40.0%
!!! TTGGTTGGTTGTTTTTGGAG+GGG - chr8.1:65983826-65983845 MS.gene056170:intron 40.0%
ACACGGGAATCAATACCCTT+TGG + chr8.1:65984650-65984669 None:intergenic 45.0%
ATAAGCAACACGAGCACAAG+GGG + chr8.1:65984365-65984384 None:intergenic 45.0%
GATGATGCACAACAGGTAAC+TGG - chr8.1:65984799-65984818 MS.gene056170:intron 45.0%
TGAACGCACAGATAAAGGTG+AGG + chr8.1:65984082-65984101 None:intergenic 45.0%
TGTCTCCAGATTTCAGGCAA+AGG - chr8.1:65984397-65984416 MS.gene056170:intron 45.0%
TGTGTCAACCAGGAAAAGGA+AGG - chr8.1:65984930-65984949 MS.gene056170:intron 45.0%
! AACGATCTCCTTCCTTTTCC+TGG + chr8.1:65984941-65984960 None:intergenic 45.0%
! ATCCATTTGGCAGATAGTGG+CGG + chr8.1:65984736-65984755 None:intergenic 45.0%
! CAAGGGGATGTTTGGTTCAA+CGG + chr8.1:65984349-65984368 None:intergenic 45.0%
! TACACATGTGTTTGGTTCGC+GGG + chr8.1:65984309-65984328 None:intergenic 45.0%
! TTCATTTCCAAGGGCGTGTT+TGG - chr8.1:65983807-65983826 MS.gene056170:intron 45.0%
!! AGGGTATTGATTCCCGTGTT+AGG - chr8.1:65984651-65984670 MS.gene056170:intron 45.0%
!!! TTGGTTGTTTTTGGAGGGGA+GGG - chr8.1:65983830-65983849 MS.gene056170:intron 45.0%
AGCGTGTCAAGAGGTTCATC+AGG - chr8.1:65983384-65983403 MS.gene056170:CDS 50.0%
ATATGCAGCAAAGCTGGCGT+AGG - chr8.1:65984624-65984643 MS.gene056170:intron 50.0%
CGTTGAACGTGCAGCTCAAT+TGG - chr8.1:65984957-65984976 MS.gene056170:CDS 50.0%
TCATCAGGCCTCAAAGTGAC+AGG - chr8.1:65983399-65983418 MS.gene056170:CDS 50.0%
TCGCACACAATGTGTCAACC+AGG - chr8.1:65984920-65984939 MS.gene056170:intron 50.0%
TGTGCACAACGTCCAGGATT+TGG - chr8.1:65984769-65984788 MS.gene056170:intron 50.0%
! ACACATGTGTTTGGTTCGCG+GGG + chr8.1:65984308-65984327 None:intergenic 50.0%
! GGATGTTTGGTTCAACGGAG+AGG + chr8.1:65984344-65984363 None:intergenic 50.0%
! GTTTGGTTCAACGGAGAGGA+GGG + chr8.1:65984340-65984359 None:intergenic 50.0%
! TCCATTTGGCAGATAGTGGC+GGG + chr8.1:65984735-65984754 None:intergenic 50.0%
! TGTTTGGTTCAACGGAGAGG+AGG + chr8.1:65984341-65984360 None:intergenic 50.0%
! TTTCCAAGGGCGTGTTTGGT+TGG - chr8.1:65983811-65983830 MS.gene056170:intron 50.0%
!! GTTTGTTGTGCACAACGTCC+AGG - chr8.1:65984763-65984782 MS.gene056170:intron 50.0%
!!! GTTGGTTGTTTTTGGAGGGG+AGG - chr8.1:65983829-65983848 MS.gene056170:intron 50.0%
!!! TGGTTGTTTTTGGAGGGGAG+GGG - chr8.1:65983831-65983850 MS.gene056170:intron 50.0%
ACCCGCCACTATCTGCCAAA+TGG - chr8.1:65984731-65984750 MS.gene056170:intron 55.0%
CAAAGCTGGCGTAGGCCAAA+GGG - chr8.1:65984632-65984651 MS.gene056170:intron 55.0%
CAACCAACCAAACACGCCCT+TGG + chr8.1:65983817-65983836 None:intergenic 55.0%
CACGAGCACAAGGGGATGTT+TGG + chr8.1:65984357-65984376 None:intergenic 55.0%
GTCTTCTTGACTGCGCAACC+TGG + chr8.1:65985007-65985026 None:intergenic 55.0%
TTGTCACCAACAAGCTCGCC+AGG - chr8.1:65984986-65985005 MS.gene056170:CDS 55.0%
! CACATGTGTTTGGTTCGCGG+GGG + chr8.1:65984307-65984326 None:intergenic 55.0%
GCAAAGCTGGCGTAGGCCAA+AGG - chr8.1:65984631-65984650 MS.gene056170:intron 60.0%
GCGCAACCTGGCGAGCTTGT+TGG + chr8.1:65984995-65985014 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 65983347 65985032 65983347 ID=MS.gene056170
chr8.1 mRNA 65983347 65985032 65983347 ID=MS.gene056170.t1;Parent=MS.gene056170
chr8.1 exon 65984943 65985032 65984943 ID=MS.gene056170.t1.exon1;Parent=MS.gene056170.t1
chr8.1 CDS 65984943 65985032 65984943 ID=cds.MS.gene056170.t1;Parent=MS.gene056170.t1
chr8.1 exon 65983566 65983747 65983566 ID=MS.gene056170.t1.exon2;Parent=MS.gene056170.t1
chr8.1 CDS 65983566 65983747 65983566 ID=cds.MS.gene056170.t1;Parent=MS.gene056170.t1
chr8.1 exon 65983347 65983476 65983347 ID=MS.gene056170.t1.exon3;Parent=MS.gene056170.t1
chr8.1 CDS 65983347 65983476 65983347 ID=cds.MS.gene056170.t1;Parent=MS.gene056170.t1
Gene Sequence

>MS.gene056170

ATGGCTGTTCCTCTGCTAACCAAGAAGATTATCAAGAAGCGTGTCAAGAGGTTCATCAGGCCTCAAAGTGACAGGAAAATATGTGTCAAGGTATGTCAATGTTATGTGCAAAAATAAAATTGTTGCTTATATATGGTAGAAATTTAGGGCCTGTTTCGAATGACCTATTTGAGCTTTTCTCCTTATATATGTTTTGTGAAACTTTCTGGGAGTACTAATGAAAACAGCTTTTAACAATTTTCACAAGCTTTTTCATGTTATAGCTTATGTAAGATTATGCGTCTGCTCTATAAGCTGCAAATAAGCTCTTTATCCAAACATGCCCTTAATGTGTTTAGAAATATGTTGGAGAGACCTCAAAGGTATGCTTGAGCTTTCTCCAACAGATTCCTAGATACACTACAAATACGTATAGATTGAATGTTATTATAAAAGTCTTTCAGGGTAAGTAGAAATGCTTTTCATTTCCAAGGGCGTGTTTGGTTGGTTGTTTTTGGAGGGGAGGGGATGACTTACATGACGTTTTTTTGGTTCAATTATTTTTGGTGGGGAGGGAATATAGAGCAATTTTTTGAAGGGGAGTGAAGTGATGCTAAAATGCGAATTAATATAATGATATTTACCCTCAATTTTGTTTTGTTAAAACTCTAAATTATCCTTTGTTGGTGAGTTAGATATTTGGATTTGTTTCGTCTCAATTGTGAAAAAAACTTGAGAGGGTTATGTGAATAACCTCACCTTTATCTGTGCGTTCAATTAATTTTCTTGCTTATTGTTTTGTTTCATGTCAATTCATTTTTTCTTCCAAATACCTCTTTCATTTTGTTACTGTTTTGTTTTGTGAATTTCGTTTTGGTGCTTACTTCTTTATTGTGTTAATTTCGTTTATATAATAAGACAAACATGTTTTCATATGACAAGTGCTGTGAGGATAATAAGCTAAATTGGTTTTAAAATCCCCCGCGAACCAAACACATGTGTAATTAAATTCCCTCCTCTCCGTTGAACCAAACATCCCCTTGTGCTCGTGTTGCTTATTTTACATAACAATGTCTCCAGATTTCAGGCAAAGGTTTATTGTGTCACTTCCATGTTTTGTCCTTGTACCAATTTTTCTATTGCATTTTGTAATACAAAAACCTCTTTGATGTTCCTACTGATTCCTGTTACCTTTTATTTTTTTACTCTCATTCTATTTGCTGTTCTGTGCATACAGTTTGTATATGTTTTGCAAATTGAATCCAGGTTTATAATATTTTAACATTGTTACTGTTACAATATGCAGCAAAGCTGGCGTAGGCCAAAGGGTATTGATTCCCGTGTTAGGAGAAAATTCAAAGGAGTTACTTTGATGCCAAACATTGGATATGGCTCAGACAAGAAGACCCGCCACTATCTGCCAAATGGATTCAAGAAGTTTGTTGTGCACAACGTCCAGGATTTGGAACTTTTGATGATGCACAACAGGTAACTGGTTTTAGTTTTGCATTTTGATTCTTAATTTCTCATTGACCCTTAGTAGCTGCAATTCTGACTTTGTTTATTTACATCTTCAGGACATACTGTGCTGAGATCGCACACAATGTGTCAACCAGGAAAAGGAAGGAGATCGTTGAACGTGCAGCTCAATTGGATGTTGTTGTCACCAACAAGCTCGCCAGGTTGCGCAGTCAAGAAGACGAGTGA

Protein sequence

>MS.gene056170.t1

MAVPLLTKKIIKKRVKRFIRPQSDRKICVKQSWRRPKGIDSRVRRKFKGVTLMPNIGYGSDKKTRHYLPNGFKKFVVHNVQDLELLMMHNRTYCAEIAHNVSTRKRKEIVERAAQLDVVVTNKLARLRSQEDE