Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027346.t1 | XP_024625350.1 | 99.2 | 133 | 1 | 0 | 1 | 133 | 1 | 133 | 9.30E-67 | 262.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027346.t1 | P49211 | 88.7 | 133 | 15 | 0 | 1 | 133 | 1 | 133 | 1.2e-62 | 240.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027346.t1 | I3SPQ1 | 99.2 | 133 | 1 | 0 | 1 | 133 | 1 | 133 | 6.7e-67 | 262.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene027346 | MS.gene011050 | PPI |
| MS.gene016666 | MS.gene027346 | PPI |
| MS.gene45749 | MS.gene027346 | PPI |
| MS.gene027346 | MS.gene95251 | PPI |
| MS.gene056170 | MS.gene027346 | PPI |
| MS.gene08771 | MS.gene027346 | PPI |
| MS.gene027346 | MS.gene051274 | PPI |
| MS.gene027346 | MS.gene023035 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027346.t1 | MTR_7g053180 | 99.248 | 133 | 1 | 0 | 1 | 133 | 1 | 133 | 4.00e-96 | 272 |
| MS.gene027346.t1 | MTR_7g007920 | 97.744 | 133 | 3 | 0 | 1 | 133 | 1 | 133 | 2.40e-94 | 268 |
| MS.gene027346.t1 | MTR_8g085740 | 91.729 | 133 | 11 | 0 | 1 | 133 | 1 | 133 | 1.02e-87 | 251 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027346.t1 | AT4G18100 | 88.722 | 133 | 15 | 0 | 1 | 133 | 1 | 133 | 7.89e-88 | 251 |
| MS.gene027346.t1 | AT5G46430 | 86.466 | 133 | 18 | 0 | 1 | 133 | 1 | 133 | 6.93e-87 | 249 |
| MS.gene027346.t1 | AT5G46430 | 86.466 | 133 | 18 | 0 | 1 | 133 | 1 | 133 | 6.93e-87 | 249 |
Find 31 sgRNAs with CRISPR-Local
Find 61 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACGCTTCTTCACAATCTTCT+TGG | 0.281194 | 7.4:+50032390 | None:intergenic |
| CGTTGAACGTGCAGCACAAT+TGG | 0.339452 | 7.4:-50031414 | MS.gene027346:CDS |
| ACCCGCCACTATTTGCCTAA+TGG | 0.370226 | 7.4:-50031638 | MS.gene027346:CDS |
| AGGGTATTGATTCACGAGTT+AGG | 0.379946 | 7.4:-50031718 | MS.gene027346:CDS |
| CAAAGCTGGCGCAGGCCAAA+GGG | 0.395273 | 7.4:-50031737 | MS.gene027346:CDS |
| GCGCAACCTGGCCAGCTTGT+TGG | 0.406434 | 7.4:+50031379 | None:intergenic |
| GCAAAGCTGGCGCAGGCCAA+AGG | 0.427216 | 7.4:-50031738 | MS.gene027346:CDS |
| TTGTCACCAACAAGCTGGCC+AGG | 0.462847 | 7.4:-50031385 | MS.gene027346:CDS |
| TCATCAAAAGCTCCAGGTCC+TGG | 0.466322 | 7.4:+50031588 | None:intergenic |
| GTATGCAGCAAAGCTGGCGC+AGG | 0.468387 | 7.4:-50031745 | MS.gene027346:intron |
| ACGAACTTCTTGAATCCATT+AGG | 0.471582 | 7.4:+50031623 | None:intergenic |
| TGAATCCATTAGGCAAATAG+TGG | 0.478886 | 7.4:+50031633 | None:intergenic |
| AGCGTGTCAAGAGGTTCATC+AGG | 0.489670 | 7.4:-50032372 | MS.gene027346:CDS |
| TTGATGCCTAACATTGGATA+CGG | 0.494285 | 7.4:-50031674 | MS.gene027346:CDS |
| ACAATCTTCTTGGTGAGCAA+TGG | 0.494679 | 7.4:+50032400 | None:intergenic |
| ACTCGTGAATCAATACCCTT+TGG | 0.495359 | 7.4:+50031722 | None:intergenic |
| TCTGAGCCGTATCCAATGTT+AGG | 0.508029 | 7.4:+50031668 | None:intergenic |
| GTTCGTCGTGCACAATGTCC+AGG | 0.509201 | 7.4:-50031606 | MS.gene027346:CDS |
| TCATCAGGCCACAAAGTGAC+AGG | 0.512111 | 7.4:-50032357 | MS.gene027346:CDS |
| CGTGCACAATGTCCAGGACC+TGG | 0.533636 | 7.4:-50031600 | MS.gene027346:CDS |
| GTTACTTTGATGCCTAACAT+TGG | 0.534023 | 7.4:-50031680 | MS.gene027346:CDS |
| GTCTTCTTGACTGCGCAACC+TGG | 0.545129 | 7.4:+50031367 | None:intergenic |
| GTTGTGGTATGCAGCAAAGC+TGG | 0.550030 | 7.4:-50031751 | MS.gene027346:intron |
| TCCATTAGGCAAATAGTGGC+GGG | 0.556875 | 7.4:+50031637 | None:intergenic |
| TGTGCATCATCAAAAGCTCC+AGG | 0.581714 | 7.4:+50031582 | None:intergenic |
| ATCCATTAGGCAAATAGTGG+CGG | 0.593712 | 7.4:+50031636 | None:intergenic |
| CGAGTTAGGAGAAAATTCAA+AGG | 0.594861 | 7.4:-50031704 | MS.gene027346:CDS |
| TGACAGGAAAATATGTGTGA+AGG | 0.596948 | 7.4:-50032341 | MS.gene027346:intron |
| TGTTGTTGTCACCAACAAGC+TGG | 0.626162 | 7.4:-50031390 | MS.gene027346:CDS |
| TTGTGAAGAAGCGTGTCAAG+AGG | 0.636300 | 7.4:-50032381 | MS.gene027346:CDS |
| CTTCTTGGTGAGCAATGGAA+CGG | 0.637090 | 7.4:+50032405 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATACAGAACAACAAATAAAA+TGG | + | chr7.4:50031936-50031955 | None:intergenic | 20.0% |
| !! | CAAATACAGCAAAAATAATT+AGG | + | chr7.4:50031798-50031817 | None:intergenic | 20.0% |
| !!! | AATAGATTAGCTTTCAAAAA+TGG | - | chr7.4:50031460-50031479 | MS.gene027346:CDS | 20.0% |
| ! | AGAACAACAAATAAAATGGA+AGG | + | chr7.4:50031932-50031951 | None:intergenic | 25.0% |
| ! | AGATTGGATGTTATAATTTC+AGG | - | chr7.4:50031657-50031676 | MS.gene027346:CDS | 25.0% |
| ! | ATCAAATGATTTAGTGTGTT+TGG | - | chr7.4:50031528-50031547 | MS.gene027346:intron | 25.0% |
| ! | GATTGGATGTTATAATTTCA+GGG | - | chr7.4:50031658-50031677 | MS.gene027346:CDS | 25.0% |
| !! | CATTTTCAGCAAGAATAATT+AGG | + | chr7.4:50031720-50031739 | None:intergenic | 25.0% |
| ACAAATAAAATGGAAGGAGT+AGG | + | chr7.4:50031926-50031945 | None:intergenic | 30.0% | |
| ! | GTCATTTCCATATTTTGTCA+GGG | - | chr7.4:50031849-50031868 | MS.gene027346:intron | 30.0% |
| !! | ATTTGTTGTTCTGTATATGC+TGG | - | chr7.4:50031940-50031959 | MS.gene027346:intron | 30.0% |
| AAAGCTCAAACATATCACGA+CGG | + | chr7.4:50031570-50031589 | None:intergenic | 35.0% | |
| ACGAACTTCTTGAATCCATT+AGG | + | chr7.4:50032150-50032169 | None:intergenic | 35.0% | |
| AGGTACATGAGCGTAATAAA+TGG | + | chr7.4:50031700-50031719 | None:intergenic | 35.0% | |
| AGTTACTAAGAGACAATGAG+AGG | + | chr7.4:50032251-50032270 | None:intergenic | 35.0% | |
| ATAAAATGGAAGGAGTAGGT+AGG | + | chr7.4:50031922-50031941 | None:intergenic | 35.0% | |
| ATTGCTACCCTGACAAAATA+TGG | + | chr7.4:50031859-50031878 | None:intergenic | 35.0% | |
| CACTATGAACACTTGTAGAT+TGG | - | chr7.4:50031641-50031660 | MS.gene027346:CDS | 35.0% | |
| CATTTACATGAACAATCCAC+AGG | - | chr7.4:50032280-50032299 | MS.gene027346:intron | 35.0% | |
| CGAGTTAGGAGAAAATTCAA+AGG | - | chr7.4:50032066-50032085 | MS.gene027346:intron | 35.0% | |
| TAGTGTGTTTGGAAAACTGT+TGG | - | chr7.4:50031539-50031558 | MS.gene027346:intron | 35.0% | |
| TGAATCCATTAGGCAAATAG+TGG | + | chr7.4:50032140-50032159 | None:intergenic | 35.0% | |
| TGACAGGAAAATATGTGTGA+AGG | - | chr7.4:50031429-50031448 | MS.gene027346:CDS | 35.0% | |
| TGCATACCACAACAGTAATA+AGG | + | chr7.4:50032012-50032031 | None:intergenic | 35.0% | |
| TGTTTGGAAAACTGTTGGAT+AGG | - | chr7.4:50031544-50031563 | MS.gene027346:intron | 35.0% | |
| TTCTGACCTTATTACTGTTG+TGG | - | chr7.4:50032003-50032022 | MS.gene027346:intron | 35.0% | |
| TTGATGCCTAACATTGGATA+CGG | - | chr7.4:50032096-50032115 | MS.gene027346:intron | 35.0% | |
| ! | AACGATCTCCTTTCTTTTTC+GGG | + | chr7.4:50032340-50032359 | None:intergenic | 35.0% |
| ! | CAACGATCTCCTTTCTTTTT+CGG | + | chr7.4:50032341-50032360 | None:intergenic | 35.0% |
| ! | CGTCATTTCCATATTTTGTC+AGG | - | chr7.4:50031848-50031867 | MS.gene027346:intron | 35.0% |
| ! | GTTACTTTGATGCCTAACAT+TGG | - | chr7.4:50032090-50032109 | MS.gene027346:intron | 35.0% |
| !!! | CATATTTTCCTGTCACTTTG+TGG | + | chr7.4:50031424-50031443 | None:intergenic | 35.0% |
| ACAATCTTCTTGGTGAGCAA+TGG | + | chr7.4:50031373-50031392 | None:intergenic | 40.0% | |
| ACGCTTCTTCACAATCTTCT+TGG | + | chr7.4:50031383-50031402 | None:intergenic | 40.0% | |
| ACTCGTGAATCAATACCCTT+TGG | + | chr7.4:50032051-50032070 | None:intergenic | 40.0% | |
| ATCCATTAGGCAAATAGTGG+CGG | + | chr7.4:50032137-50032156 | None:intergenic | 40.0% | |
| TGTGTCAACCCGAAAAAGAA+AGG | - | chr7.4:50032329-50032348 | MS.gene027346:intron | 40.0% | |
| !! | AGGGTATTGATTCACGAGTT+AGG | - | chr7.4:50032052-50032071 | MS.gene027346:intron | 40.0% |
| !!! | AGCTTTTGATGATGCACAAC+AGG | - | chr7.4:50032193-50032212 | MS.gene027346:intron | 40.0% |
| CTTCTTGGTGAGCAATGGAA+CGG | + | chr7.4:50031368-50031387 | None:intergenic | 45.0% | |
| GATGATGCACAACAGGTAAC+TGG | - | chr7.4:50032200-50032219 | MS.gene027346:intron | 45.0% | |
| TCCATTAGGCAAATAGTGGC+GGG | + | chr7.4:50032136-50032155 | None:intergenic | 45.0% | |
| TCTGAGCCGTATCCAATGTT+AGG | + | chr7.4:50032105-50032124 | None:intergenic | 45.0% | |
| TGTGCATCATCAAAAGCTCC+AGG | + | chr7.4:50032191-50032210 | None:intergenic | 45.0% | |
| TGTTGTTGTCACCAACAAGC+TGG | - | chr7.4:50032380-50032399 | MS.gene027346:CDS | 45.0% | |
| ! | TTGTGAAGAAGCGTGTCAAG+AGG | - | chr7.4:50031389-50031408 | MS.gene027346:CDS | 45.0% |
| ACCCGCCACTATTTGCCTAA+TGG | - | chr7.4:50032132-50032151 | MS.gene027346:intron | 50.0% | |
| AGCGTGTCAAGAGGTTCATC+AGG | - | chr7.4:50031398-50031417 | MS.gene027346:CDS | 50.0% | |
| CGTTGAACGTGCAGCACAAT+TGG | - | chr7.4:50032356-50032375 | MS.gene027346:CDS | 50.0% | |
| GTTGTGGTATGCAGCAAAGC+TGG | - | chr7.4:50032019-50032038 | MS.gene027346:intron | 50.0% | |
| TCATCAAAAGCTCCAGGTCC+TGG | + | chr7.4:50032185-50032204 | None:intergenic | 50.0% | |
| TCATCAGGCCACAAAGTGAC+AGG | - | chr7.4:50031413-50031432 | MS.gene027346:CDS | 50.0% | |
| TCTCAGCACAGTATGTCCTG+TGG | + | chr7.4:50032299-50032318 | None:intergenic | 50.0% | |
| GTCTTCTTGACTGCGCAACC+TGG | + | chr7.4:50032406-50032425 | None:intergenic | 55.0% | |
| GTTCGTCGTGCACAATGTCC+AGG | - | chr7.4:50032164-50032183 | MS.gene027346:intron | 55.0% | |
| TTGTCACCAACAAGCTGGCC+AGG | - | chr7.4:50032385-50032404 | MS.gene027346:CDS | 55.0% | |
| CAAAGCTGGCGCAGGCCAAA+GGG | - | chr7.4:50032033-50032052 | MS.gene027346:intron | 60.0% | |
| CGTGCACAATGTCCAGGACC+TGG | - | chr7.4:50032170-50032189 | MS.gene027346:intron | 60.0% | |
| GTATGCAGCAAAGCTGGCGC+AGG | - | chr7.4:50032025-50032044 | MS.gene027346:intron | 60.0% | |
| GCAAAGCTGGCGCAGGCCAA+AGG | - | chr7.4:50032032-50032051 | MS.gene027346:intron | 65.0% | |
| GCGCAACCTGGCCAGCTTGT+TGG | + | chr7.4:50032394-50032413 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 50031361 | 50032431 | 50031361 | ID=MS.gene027346 |
| chr7.4 | mRNA | 50031361 | 50032431 | 50031361 | ID=MS.gene027346.t1;Parent=MS.gene027346 |
| chr7.4 | exon | 50032342 | 50032431 | 50032342 | ID=MS.gene027346.t1.exon1;Parent=MS.gene027346.t1 |
| chr7.4 | CDS | 50032342 | 50032431 | 50032342 | ID=cds.MS.gene027346.t1;Parent=MS.gene027346.t1 |
| chr7.4 | exon | 50031578 | 50031759 | 50031578 | ID=MS.gene027346.t1.exon2;Parent=MS.gene027346.t1 |
| chr7.4 | CDS | 50031578 | 50031759 | 50031578 | ID=cds.MS.gene027346.t1;Parent=MS.gene027346.t1 |
| chr7.4 | exon | 50031361 | 50031490 | 50031361 | ID=MS.gene027346.t1.exon3;Parent=MS.gene027346.t1 |
| chr7.4 | CDS | 50031361 | 50031490 | 50031361 | ID=cds.MS.gene027346.t1;Parent=MS.gene027346.t1 |
| Gene Sequence |
| Protein sequence |
