Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051274.t1 | XP_024625350.1 | 99.2 | 133 | 1 | 0 | 1 | 133 | 1 | 133 | 9.30E-67 | 262.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051274.t1 | P49211 | 88.7 | 133 | 15 | 0 | 1 | 133 | 1 | 133 | 1.2e-62 | 240.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051274.t1 | I3SPQ1 | 99.2 | 133 | 1 | 0 | 1 | 133 | 1 | 133 | 6.7e-67 | 262.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051274 | MS.gene053148 | 0.807197 | 5.68E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene023035 | MS.gene051274 | PPI |
| MS.gene95251 | MS.gene051274 | PPI |
| MS.gene016666 | MS.gene051274 | PPI |
| MS.gene45749 | MS.gene051274 | PPI |
| MS.gene056170 | MS.gene051274 | PPI |
| MS.gene08771 | MS.gene051274 | PPI |
| MS.gene027346 | MS.gene051274 | PPI |
| MS.gene051274 | MS.gene011050 | PPI |
| MS.gene011050 | MS.gene051274 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051274.t1 | MTR_7g053180 | 99.248 | 133 | 1 | 0 | 1 | 133 | 1 | 133 | 4.00e-96 | 272 |
| MS.gene051274.t1 | MTR_7g007920 | 97.744 | 133 | 3 | 0 | 1 | 133 | 1 | 133 | 2.40e-94 | 268 |
| MS.gene051274.t1 | MTR_8g085740 | 91.729 | 133 | 11 | 0 | 1 | 133 | 1 | 133 | 1.02e-87 | 251 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051274.t1 | AT4G18100 | 88.722 | 133 | 15 | 0 | 1 | 133 | 1 | 133 | 7.89e-88 | 251 |
| MS.gene051274.t1 | AT5G46430 | 86.466 | 133 | 18 | 0 | 1 | 133 | 1 | 133 | 6.93e-87 | 249 |
| MS.gene051274.t1 | AT5G46430 | 86.466 | 133 | 18 | 0 | 1 | 133 | 1 | 133 | 6.93e-87 | 249 |
Find 32 sgRNAs with CRISPR-Local
Find 56 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACGCTTCTTCACAATCTTCT+TGG | 0.281194 | 7.3:+50917009 | None:intergenic |
| CGTTGAACGTGCGGCACAAT+TGG | 0.294789 | 7.3:-50916078 | MS.gene051274:CDS |
| ACCCGCCACTATTTGCCTAA+TGG | 0.370226 | 7.3:-50916300 | MS.gene051274:CDS |
| AGGGTATTGATTCACGAGTT+AGG | 0.379946 | 7.3:-50916380 | MS.gene051274:CDS |
| CAAAGCTGGCGCAGGCCAAA+GGG | 0.395273 | 7.3:-50916399 | MS.gene051274:CDS |
| GCGCAACCTGGCCAGCTTGT+TGG | 0.406434 | 7.3:+50916043 | None:intergenic |
| GCAAAGCTGGCGCAGGCCAA+AGG | 0.427216 | 7.3:-50916400 | MS.gene051274:CDS |
| ACAAACTTCTTGAATCCATT+AGG | 0.438510 | 7.3:+50916285 | None:intergenic |
| TTGTCACCAACAAGCTGGCC+AGG | 0.462847 | 7.3:-50916049 | MS.gene051274:CDS |
| GTATGCAGCAAAGCTGGCGC+AGG | 0.468387 | 7.3:-50916407 | MS.gene051274:intron |
| TGAATCCATTAGGCAAATAG+TGG | 0.478886 | 7.3:+50916295 | None:intergenic |
| TCATCAAAAGTTCCAGGTCC+TGG | 0.486161 | 7.3:+50916250 | None:intergenic |
| AGCGTGTCAAGAGGTTCATC+AGG | 0.489670 | 7.3:-50916991 | MS.gene051274:CDS |
| TTGATGCCTAACATTGGATA+CGG | 0.494285 | 7.3:-50916336 | MS.gene051274:CDS |
| ACAATCTTCTTGGTGAGCAA+TGG | 0.494679 | 7.3:+50917019 | None:intergenic |
| ACTCGTGAATCAATACCCTT+TGG | 0.495359 | 7.3:+50916384 | None:intergenic |
| GTTTGTCGTGCACAATGTCC+AGG | 0.506762 | 7.3:-50916268 | MS.gene051274:CDS |
| TCTGAGCCGTATCCAATGTT+AGG | 0.508029 | 7.3:+50916330 | None:intergenic |
| TCATCAGGCCACAAAGTGAC+AGG | 0.512111 | 7.3:-50916976 | MS.gene051274:CDS |
| TGTGCATCATCAAAAGTTCC+AGG | 0.513705 | 7.3:+50916244 | None:intergenic |
| GTTACTTTGATGCCTAACAT+TGG | 0.534023 | 7.3:-50916342 | MS.gene051274:CDS |
| GTCTTCTTGACTGCGCAACC+TGG | 0.545129 | 7.3:+50916031 | None:intergenic |
| GTTGTGGTATGCAGCAAAGC+TGG | 0.550030 | 7.3:-50916413 | MS.gene051274:intron |
| TCCATTAGGCAAATAGTGGC+GGG | 0.556875 | 7.3:+50916299 | None:intergenic |
| CGTGCACAATGTCCAGGACC+TGG | 0.559607 | 7.3:-50916262 | MS.gene051274:CDS |
| ATCCATTAGGCAAATAGTGG+CGG | 0.593712 | 7.3:+50916298 | None:intergenic |
| CGAGTTAGGAGAAAATTCAA+AGG | 0.594861 | 7.3:-50916366 | MS.gene051274:CDS |
| TGACAGGAAAATATGTGTGA+AGG | 0.596948 | 7.3:-50916960 | MS.gene051274:intron |
| AAAGGAGATCGTTGAACGTG+CGG | 0.614630 | 7.3:-50916087 | MS.gene051274:CDS |
| TGTTGTTGTCACCAACAAGC+TGG | 0.626162 | 7.3:-50916054 | MS.gene051274:CDS |
| TTGTGAAGAAGCGTGTCAAG+AGG | 0.636300 | 7.3:-50917000 | MS.gene051274:CDS |
| CTTCTTGGTGAGCAATGGAA+CGG | 0.637090 | 7.3:+50917024 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATGGAAGTAAAAAATAAC+AGG | + | chr7.3:50916552-50916571 | None:intergenic | 20.0% |
| !! | AGATTGAATGTTATAATTTC+AGG | - | chr7.3:50916267-50916286 | MS.gene051274:CDS | 20.0% |
| !! | ATACAGAACAACAAATAAAA+TGG | + | chr7.3:50916569-50916588 | None:intergenic | 20.0% |
| !! | GATTGAATGTTATAATTTCA+GGG | - | chr7.3:50916268-50916287 | MS.gene051274:CDS | 20.0% |
| !! | TTCATGTAAATATCAGAATA+TGG | + | chr7.3:50916891-50916910 | None:intergenic | 20.0% |
| !!! | AATAGATTAGCTTTCAAAAA+TGG | - | chr7.3:50916124-50916143 | MS.gene051274:CDS | 20.0% |
| !!! | CATTTTCAGCAAAAATAATT+AGG | + | chr7.3:50916408-50916427 | None:intergenic | 20.0% |
| ! | AAAAAATAACAGGTATCAGT+AGG | + | chr7.3:50916542-50916561 | None:intergenic | 25.0% |
| ! | ATCAAATGATTTAGTGTGTT+TGG | - | chr7.3:50916192-50916211 | MS.gene051274:intron | 25.0% |
| !! | CATTTTCAGCAAGAATAATT+AGG | + | chr7.3:50916330-50916349 | None:intergenic | 25.0% |
| ACAAACTTCTTGAATCCATT+AGG | + | chr7.3:50916771-50916790 | None:intergenic | 30.0% | |
| ATTGCTACACTGACAAAATA+TGG | + | chr7.3:50916469-50916488 | None:intergenic | 30.0% | |
| TATTTACATGAACAAACCTC+AGG | - | chr7.3:50916899-50916918 | MS.gene051274:intron | 30.0% | |
| !! | ATTTGTTGTTCTGTATATGC+TGG | - | chr7.3:50916573-50916592 | MS.gene051274:intron | 30.0% |
| AGGTACATGAGCGTAATAAA+TGG | + | chr7.3:50916310-50916329 | None:intergenic | 35.0% | |
| CGAGTTAGGAGAAAATTCAA+AGG | - | chr7.3:50916687-50916706 | MS.gene051274:intron | 35.0% | |
| TGAATCCATTAGGCAAATAG+TGG | + | chr7.3:50916761-50916780 | None:intergenic | 35.0% | |
| TGACAGGAAAATATGTGTGA+AGG | - | chr7.3:50916093-50916112 | MS.gene051274:CDS | 35.0% | |
| TGCATACCACAACAGTAATA+AGG | + | chr7.3:50916633-50916652 | None:intergenic | 35.0% | |
| TGTGTCAACCAGAAAAAGAA+AGG | - | chr7.3:50916948-50916967 | MS.gene051274:intron | 35.0% | |
| TTCTGACCTTATTACTGTTG+TGG | - | chr7.3:50916624-50916643 | MS.gene051274:intron | 35.0% | |
| TTGATGCCTAACATTGGATA+CGG | - | chr7.3:50916717-50916736 | MS.gene051274:intron | 35.0% | |
| ! | AACGATCTCCTTTCTTTTTC+TGG | + | chr7.3:50916959-50916978 | None:intergenic | 35.0% |
| ! | GTTACTTTGATGCCTAACAT+TGG | - | chr7.3:50916711-50916730 | MS.gene051274:intron | 35.0% |
| !!! | AACTTTTGATGATGCACAAC+AGG | - | chr7.3:50916814-50916833 | MS.gene051274:intron | 35.0% |
| !!! | CATATTTTCCTGTCACTTTG+TGG | + | chr7.3:50916088-50916107 | None:intergenic | 35.0% |
| ACAATCTTCTTGGTGAGCAA+TGG | + | chr7.3:50916037-50916056 | None:intergenic | 40.0% | |
| ACGCTTCTTCACAATCTTCT+TGG | + | chr7.3:50916047-50916066 | None:intergenic | 40.0% | |
| ACTCGTGAATCAATACCCTT+TGG | + | chr7.3:50916672-50916691 | None:intergenic | 40.0% | |
| ATCCATTAGGCAAATAGTGG+CGG | + | chr7.3:50916758-50916777 | None:intergenic | 40.0% | |
| TAACAGGTATCAGTAGGAGT+AGG | + | chr7.3:50916536-50916555 | None:intergenic | 40.0% | |
| TGTGCATCATCAAAAGTTCC+AGG | + | chr7.3:50916812-50916831 | None:intergenic | 40.0% | |
| !! | AGGGTATTGATTCACGAGTT+AGG | - | chr7.3:50916673-50916692 | MS.gene051274:intron | 40.0% |
| AAAGGAGATCGTTGAACGTG+CGG | - | chr7.3:50916966-50916985 | MS.gene051274:CDS | 45.0% | |
| AGGTATCAGTAGGAGTAGGT+AGG | + | chr7.3:50916532-50916551 | None:intergenic | 45.0% | |
| CTTCTTGGTGAGCAATGGAA+CGG | + | chr7.3:50916032-50916051 | None:intergenic | 45.0% | |
| GATGATGCACAACAGGTAAC+TGG | - | chr7.3:50916821-50916840 | MS.gene051274:intron | 45.0% | |
| TCATCAAAAGTTCCAGGTCC+TGG | + | chr7.3:50916806-50916825 | None:intergenic | 45.0% | |
| TCCATTAGGCAAATAGTGGC+GGG | + | chr7.3:50916757-50916776 | None:intergenic | 45.0% | |
| TCTGAGCCGTATCCAATGTT+AGG | + | chr7.3:50916726-50916745 | None:intergenic | 45.0% | |
| TGTTGTTGTCACCAACAAGC+TGG | - | chr7.3:50916999-50917018 | MS.gene051274:CDS | 45.0% | |
| ! | TTGTGAAGAAGCGTGTCAAG+AGG | - | chr7.3:50916053-50916072 | MS.gene051274:CDS | 45.0% |
| ACCCGCCACTATTTGCCTAA+TGG | - | chr7.3:50916753-50916772 | MS.gene051274:intron | 50.0% | |
| AGCGTGTCAAGAGGTTCATC+AGG | - | chr7.3:50916062-50916081 | MS.gene051274:CDS | 50.0% | |
| GTTGTGGTATGCAGCAAAGC+TGG | - | chr7.3:50916640-50916659 | MS.gene051274:intron | 50.0% | |
| GTTTGTCGTGCACAATGTCC+AGG | - | chr7.3:50916785-50916804 | MS.gene051274:intron | 50.0% | |
| TCATCAGGCCACAAAGTGAC+AGG | - | chr7.3:50916077-50916096 | MS.gene051274:CDS | 50.0% | |
| TCTCAGCACAGTATGTCCTG+AGG | + | chr7.3:50916918-50916937 | None:intergenic | 50.0% | |
| CGTTGAACGTGCGGCACAAT+TGG | - | chr7.3:50916975-50916994 | MS.gene051274:CDS | 55.0% | |
| GTCTTCTTGACTGCGCAACC+TGG | + | chr7.3:50917025-50917044 | None:intergenic | 55.0% | |
| TTGTCACCAACAAGCTGGCC+AGG | - | chr7.3:50917004-50917023 | MS.gene051274:CDS | 55.0% | |
| CAAAGCTGGCGCAGGCCAAA+GGG | - | chr7.3:50916654-50916673 | MS.gene051274:intron | 60.0% | |
| CGTGCACAATGTCCAGGACC+TGG | - | chr7.3:50916791-50916810 | MS.gene051274:intron | 60.0% | |
| GTATGCAGCAAAGCTGGCGC+AGG | - | chr7.3:50916646-50916665 | MS.gene051274:intron | 60.0% | |
| GCAAAGCTGGCGCAGGCCAA+AGG | - | chr7.3:50916653-50916672 | MS.gene051274:intron | 65.0% | |
| GCGCAACCTGGCCAGCTTGT+TGG | + | chr7.3:50917013-50917032 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 50916025 | 50917050 | 50916025 | ID=MS.gene051274 |
| chr7.3 | mRNA | 50916025 | 50917050 | 50916025 | ID=MS.gene051274.t1;Parent=MS.gene051274 |
| chr7.3 | exon | 50916961 | 50917050 | 50916961 | ID=MS.gene051274.t1.exon1;Parent=MS.gene051274.t1 |
| chr7.3 | CDS | 50916961 | 50917050 | 50916961 | ID=cds.MS.gene051274.t1;Parent=MS.gene051274.t1 |
| chr7.3 | exon | 50916240 | 50916421 | 50916240 | ID=MS.gene051274.t1.exon2;Parent=MS.gene051274.t1 |
| chr7.3 | CDS | 50916240 | 50916421 | 50916240 | ID=cds.MS.gene051274.t1;Parent=MS.gene051274.t1 |
| chr7.3 | exon | 50916025 | 50916154 | 50916025 | ID=MS.gene051274.t1.exon3;Parent=MS.gene051274.t1 |
| chr7.3 | CDS | 50916025 | 50916154 | 50916025 | ID=cds.MS.gene051274.t1;Parent=MS.gene051274.t1 |
| Gene Sequence |
| Protein sequence |
