Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene023035.t1 | XP_024625350.1 | 99.2 | 133 | 1 | 0 | 1 | 133 | 1 | 133 | 9.30E-67 | 262.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene023035.t1 | P49211 | 88.7 | 133 | 15 | 0 | 1 | 133 | 1 | 133 | 1.2e-62 | 240.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene023035.t1 | I3SPQ1 | 99.2 | 133 | 1 | 0 | 1 | 133 | 1 | 133 | 6.7e-67 | 262.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene023035 | MS.gene051274 | PPI |
| MS.gene016666 | MS.gene023035 | PPI |
| MS.gene45749 | MS.gene023035 | PPI |
| MS.gene056170 | MS.gene023035 | PPI |
| MS.gene08771 | MS.gene023035 | PPI |
| MS.gene023035 | MS.gene011050 | PPI |
| MS.gene95251 | MS.gene023035 | PPI |
| MS.gene027346 | MS.gene023035 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene023035.t1 | MTR_7g053180 | 99.248 | 133 | 1 | 0 | 1 | 133 | 1 | 133 | 4.00e-96 | 272 |
| MS.gene023035.t1 | MTR_7g007920 | 97.744 | 133 | 3 | 0 | 1 | 133 | 1 | 133 | 2.40e-94 | 268 |
| MS.gene023035.t1 | MTR_8g085740 | 91.729 | 133 | 11 | 0 | 1 | 133 | 1 | 133 | 1.02e-87 | 251 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene023035.t1 | AT4G18100 | 88.722 | 133 | 15 | 0 | 1 | 133 | 1 | 133 | 7.89e-88 | 251 |
| MS.gene023035.t1 | AT5G46430 | 86.466 | 133 | 18 | 0 | 1 | 133 | 1 | 133 | 6.93e-87 | 249 |
| MS.gene023035.t1 | AT5G46430 | 86.466 | 133 | 18 | 0 | 1 | 133 | 1 | 133 | 6.93e-87 | 249 |
Find 32 sgRNAs with CRISPR-Local
Find 60 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACGCTTCTTCACAATCTTCT+TGG | 0.281194 | 7.2:+50202940 | None:intergenic |
| CGTTGAACGTGCGGCACAAT+TGG | 0.294789 | 7.2:-50201934 | MS.gene023035:CDS |
| ACCCGCCACTATTTGCCTAA+TGG | 0.370226 | 7.2:-50202158 | MS.gene023035:CDS |
| AGGGTATTGATTCACGAGTT+AGG | 0.379946 | 7.2:-50202238 | MS.gene023035:CDS |
| CAAAGCTGGCGCAGGCCAAA+GGG | 0.395273 | 7.2:-50202257 | MS.gene023035:CDS |
| GCGCAACCTGGCCAGCTTGT+TGG | 0.406434 | 7.2:+50201899 | None:intergenic |
| GCAAAGCTGGCGCAGGCCAA+AGG | 0.427216 | 7.2:-50202258 | MS.gene023035:CDS |
| ACAAACTTCTTGAATCCATT+AGG | 0.438510 | 7.2:+50202143 | None:intergenic |
| CTATGCAGCAAAGCTGGCGC+AGG | 0.457182 | 7.2:-50202265 | MS.gene023035:intron |
| TTGTCACCAACAAGCTGGCC+AGG | 0.462847 | 7.2:-50201905 | MS.gene023035:CDS |
| TCATCAAAAGCTCCAGGTCC+TGG | 0.466322 | 7.2:+50202108 | None:intergenic |
| GTTGTGCTATGCAGCAAAGC+TGG | 0.468798 | 7.2:-50202271 | MS.gene023035:intron |
| TCATCAGGCCACAGAGTGAC+AGG | 0.478689 | 7.2:-50202907 | MS.gene023035:CDS |
| TGAATCCATTAGGCAAATAG+TGG | 0.478886 | 7.2:+50202153 | None:intergenic |
| AGCGTGTCAAGAGGTTCATC+AGG | 0.489670 | 7.2:-50202922 | MS.gene023035:CDS |
| TTGATGCCTAACATTGGATA+CGG | 0.494285 | 7.2:-50202194 | MS.gene023035:CDS |
| ACAATCTTCTTGGTGAGCAA+TGG | 0.494679 | 7.2:+50202950 | None:intergenic |
| ACTCGTGAATCAATACCCTT+TGG | 0.495359 | 7.2:+50202242 | None:intergenic |
| GTTTGTCGTGCACAATGTCC+AGG | 0.506762 | 7.2:-50202126 | MS.gene023035:CDS |
| TCTGAGCCGTATCCAATGTT+AGG | 0.508029 | 7.2:+50202188 | None:intergenic |
| CGTGCACAATGTCCAGGACC+TGG | 0.538275 | 7.2:-50202120 | MS.gene023035:CDS |
| GTCTTCTTGACTGCGCAACC+TGG | 0.545129 | 7.2:+50201887 | None:intergenic |
| TCCATTAGGCAAATAGTGGC+GGG | 0.556875 | 7.2:+50202157 | None:intergenic |
| GTGACTTTGATGCCTAACAT+TGG | 0.577268 | 7.2:-50202200 | MS.gene023035:CDS |
| TGTGCATCATCAAAAGCTCC+AGG | 0.581714 | 7.2:+50202102 | None:intergenic |
| ATCCATTAGGCAAATAGTGG+CGG | 0.593712 | 7.2:+50202156 | None:intergenic |
| CGAGTTAGGAGAAAATTCAA+AGG | 0.594861 | 7.2:-50202224 | MS.gene023035:CDS |
| TGACAGGAAAATATGTGTGA+AGG | 0.603768 | 7.2:-50202891 | MS.gene023035:intron |
| AAAGGAGATCGTTGAACGTG+CGG | 0.614630 | 7.2:-50201943 | MS.gene023035:CDS |
| TGTTGTTGTCACCAACAAGC+TGG | 0.626162 | 7.2:-50201910 | MS.gene023035:CDS |
| TTGTGAAGAAGCGTGTCAAG+AGG | 0.636300 | 7.2:-50202931 | MS.gene023035:CDS |
| CTTCTTGGTGAGCAATGGAA+CGG | 0.637090 | 7.2:+50202955 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGATTAAATGTTATAATTTC+AGG | - | chr7.2:50202179-50202198 | MS.gene023035:CDS | 15.0% |
| !! | AGTAAAAAATAATAGATAAC+AGG | + | chr7.2:50202462-50202481 | None:intergenic | 15.0% |
| !! | ATACATAACAACAAATAAAA+TGG | + | chr7.2:50202486-50202505 | None:intergenic | 15.0% |
| !! | GATTAAATGTTATAATTTCA+GGG | - | chr7.2:50202180-50202199 | MS.gene023035:CDS | 15.0% |
| !! | TAAATGAGTGTAATAATAAA+TGG | + | chr7.2:50202301-50202320 | None:intergenic | 15.0% |
| !!! | ATTTTCAGCAAAAAATAATT+AGG | + | chr7.2:50202242-50202261 | None:intergenic | 15.0% |
| !! | AATAGATAAGCTTTCAAAAA+TGG | - | chr7.2:50201980-50201999 | MS.gene023035:CDS | 20.0% |
| ! | ATCAAATGATTTAGTGTGTT+TGG | - | chr7.2:50202050-50202069 | MS.gene023035:intron | 25.0% |
| !! | CATTTTCAGCAAAGATAATT+AGG | + | chr7.2:50202324-50202343 | None:intergenic | 25.0% |
| !!! | ATTTGTTGTTATGTATATGC+TGG | - | chr7.2:50202490-50202509 | MS.gene023035:intron | 25.0% |
| ACAAACTTCTTGAATCCATT+AGG | + | chr7.2:50202700-50202719 | None:intergenic | 30.0% | |
| ATTGCTACACTGACAAAATA+TGG | + | chr7.2:50202379-50202398 | None:intergenic | 30.0% | |
| AGGTACATGCGTGTAATAAA+TGG | + | chr7.2:50202222-50202241 | None:intergenic | 35.0% | |
| CATTTACATAAACAACCCAC+AGG | - | chr7.2:50202830-50202849 | MS.gene023035:intron | 35.0% | |
| CGAGTTAGGAGAAAATTCAA+AGG | - | chr7.2:50202616-50202635 | MS.gene023035:intron | 35.0% | |
| TAGTGTGTTTGGAAAACTGT+TGG | - | chr7.2:50202061-50202080 | MS.gene023035:intron | 35.0% | |
| TGAATCCATTAGGCAAATAG+TGG | + | chr7.2:50202690-50202709 | None:intergenic | 35.0% | |
| TGACAGGAAAATATGTGTGA+AGG | - | chr7.2:50201949-50201968 | MS.gene023035:CDS | 35.0% | |
| TGCATAGCACAACAGTAATA+AGG | + | chr7.2:50202562-50202581 | None:intergenic | 35.0% | |
| TGTGTCAACCAGAAAAAGAA+AGG | - | chr7.2:50202879-50202898 | MS.gene023035:intron | 35.0% | |
| TGTTTGGAAAACTGTTGGAT+AGG | - | chr7.2:50202066-50202085 | MS.gene023035:intron | 35.0% | |
| TTGATGCCTAACATTGGATA+CGG | - | chr7.2:50202646-50202665 | MS.gene023035:intron | 35.0% | |
| ! | AACGATCTCCTTTCTTTTTC+TGG | + | chr7.2:50202890-50202909 | None:intergenic | 35.0% |
| AAAGCTCAAACATACCACGA+AGG | + | chr7.2:50202092-50202111 | None:intergenic | 40.0% | |
| ACAATCTTCTTGGTGAGCAA+TGG | + | chr7.2:50201893-50201912 | None:intergenic | 40.0% | |
| ACGCTTCTTCACAATCTTCT+TGG | + | chr7.2:50201903-50201922 | None:intergenic | 40.0% | |
| ACTCGTGAATCAATACCCTT+TGG | + | chr7.2:50202601-50202620 | None:intergenic | 40.0% | |
| AGTTACGAAGAGACAATGAG+AGG | + | chr7.2:50202801-50202820 | None:intergenic | 40.0% | |
| ATCCATTAGGCAAATAGTGG+CGG | + | chr7.2:50202687-50202706 | None:intergenic | 40.0% | |
| GTGACTTTGATGCCTAACAT+TGG | - | chr7.2:50202640-50202659 | MS.gene023035:intron | 40.0% | |
| TAACAGGTATCAGTAGCAGT+AGG | + | chr7.2:50202446-50202465 | None:intergenic | 40.0% | |
| ! | CATATTTTCCTGTCACTCTG+TGG | + | chr7.2:50201944-50201963 | None:intergenic | 40.0% |
| !! | AGGGTATTGATTCACGAGTT+AGG | - | chr7.2:50202602-50202621 | MS.gene023035:intron | 40.0% |
| !!! | AGCTTTTGATGATGCACAAC+AGG | - | chr7.2:50202743-50202762 | MS.gene023035:intron | 40.0% |
| AAAGGAGATCGTTGAACGTG+CGG | - | chr7.2:50202897-50202916 | MS.gene023035:CDS | 45.0% | |
| AGGTATCAGTAGCAGTAGGT+AGG | + | chr7.2:50202442-50202461 | None:intergenic | 45.0% | |
| CTTCTTGGTGAGCAATGGAA+CGG | + | chr7.2:50201888-50201907 | None:intergenic | 45.0% | |
| GATGATGCACAACAGGTAAC+TGG | - | chr7.2:50202750-50202769 | MS.gene023035:intron | 45.0% | |
| TCCATTAGGCAAATAGTGGC+GGG | + | chr7.2:50202686-50202705 | None:intergenic | 45.0% | |
| TCTGAGCCGTATCCAATGTT+AGG | + | chr7.2:50202655-50202674 | None:intergenic | 45.0% | |
| TGTGCATCATCAAAAGCTCC+AGG | + | chr7.2:50202741-50202760 | None:intergenic | 45.0% | |
| TGTTGTTGTCACCAACAAGC+TGG | - | chr7.2:50202930-50202949 | MS.gene023035:CDS | 45.0% | |
| ! | TTGTGAAGAAGCGTGTCAAG+AGG | - | chr7.2:50201909-50201928 | MS.gene023035:CDS | 45.0% |
| AACTGTTGGATAGGCCTTCG+TGG | - | chr7.2:50202075-50202094 | MS.gene023035:intron | 50.0% | |
| ACCCGCCACTATTTGCCTAA+TGG | - | chr7.2:50202682-50202701 | MS.gene023035:intron | 50.0% | |
| AGCGTGTCAAGAGGTTCATC+AGG | - | chr7.2:50201918-50201937 | MS.gene023035:CDS | 50.0% | |
| CTCAGCACAGTATGTCCTGT+GGG | + | chr7.2:50202848-50202867 | None:intergenic | 50.0% | |
| GTTGTGCTATGCAGCAAAGC+TGG | - | chr7.2:50202569-50202588 | MS.gene023035:intron | 50.0% | |
| GTTTGTCGTGCACAATGTCC+AGG | - | chr7.2:50202714-50202733 | MS.gene023035:intron | 50.0% | |
| TCATCAAAAGCTCCAGGTCC+TGG | + | chr7.2:50202735-50202754 | None:intergenic | 50.0% | |
| TCTCAGCACAGTATGTCCTG+TGG | + | chr7.2:50202849-50202868 | None:intergenic | 50.0% | |
| CGTTGAACGTGCGGCACAAT+TGG | - | chr7.2:50202906-50202925 | MS.gene023035:CDS | 55.0% | |
| GTCTTCTTGACTGCGCAACC+TGG | + | chr7.2:50202956-50202975 | None:intergenic | 55.0% | |
| TCATCAGGCCACAGAGTGAC+AGG | - | chr7.2:50201933-50201952 | MS.gene023035:CDS | 55.0% | |
| TTGTCACCAACAAGCTGGCC+AGG | - | chr7.2:50202935-50202954 | MS.gene023035:CDS | 55.0% | |
| CAAAGCTGGCGCAGGCCAAA+GGG | - | chr7.2:50202583-50202602 | MS.gene023035:intron | 60.0% | |
| CGTGCACAATGTCCAGGACC+TGG | - | chr7.2:50202720-50202739 | MS.gene023035:intron | 60.0% | |
| CTATGCAGCAAAGCTGGCGC+AGG | - | chr7.2:50202575-50202594 | MS.gene023035:intron | 60.0% | |
| GCAAAGCTGGCGCAGGCCAA+AGG | - | chr7.2:50202582-50202601 | MS.gene023035:intron | 65.0% | |
| GCGCAACCTGGCCAGCTTGT+TGG | + | chr7.2:50202944-50202963 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 50201881 | 50202981 | 50201881 | ID=MS.gene023035 |
| chr7.2 | mRNA | 50201881 | 50202981 | 50201881 | ID=MS.gene023035.t1;Parent=MS.gene023035 |
| chr7.2 | exon | 50202892 | 50202981 | 50202892 | ID=MS.gene023035.t1.exon1;Parent=MS.gene023035.t1 |
| chr7.2 | CDS | 50202892 | 50202981 | 50202892 | ID=cds.MS.gene023035.t1;Parent=MS.gene023035.t1 |
| chr7.2 | exon | 50202098 | 50202279 | 50202098 | ID=MS.gene023035.t1.exon2;Parent=MS.gene023035.t1 |
| chr7.2 | CDS | 50202098 | 50202279 | 50202098 | ID=cds.MS.gene023035.t1;Parent=MS.gene023035.t1 |
| chr7.2 | exon | 50201881 | 50202010 | 50201881 | ID=MS.gene023035.t1.exon3;Parent=MS.gene023035.t1 |
| chr7.2 | CDS | 50201881 | 50202010 | 50201881 | ID=cds.MS.gene023035.t1;Parent=MS.gene023035.t1 |
| Gene Sequence |
| Protein sequence |
