Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene012931.t1 | RHN59613.1 | 100 | 115 | 0 | 0 | 1 | 115 | 32 | 146 | 1.30E-61 | 245.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene012931.t1 | Q1RMP0 | 38.2 | 110 | 67 | 1 | 7 | 115 | 13 | 122 | 1.9e-16 | 86.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene012931.t1 | G7JQD3 | 100.0 | 115 | 0 | 0 | 1 | 115 | 1 | 115 | 9.6e-62 | 245.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene26777 | MS.gene012931 | PPI |
| MS.gene43180 | MS.gene012931 | PPI |
| MS.gene68633 | MS.gene012931 | PPI |
| MS.gene047813 | MS.gene012931 | PPI |
| MS.gene022098 | MS.gene012931 | PPI |
| MS.gene053492 | MS.gene012931 | PPI |
| MS.gene39636 | MS.gene012931 | PPI |
| MS.gene064140 | MS.gene012931 | PPI |
| MS.gene030166 | MS.gene012931 | PPI |
| MS.gene26516 | MS.gene012931 | PPI |
| MS.gene97308 | MS.gene012931 | PPI |
| MS.gene95440 | MS.gene012931 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene012931.t1 | MTR_3g102420 | 100.000 | 115 | 0 | 0 | 1 | 115 | 1 | 115 | 4.90e-84 | 240 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene012931.t1 | AT3G25940 | 61.739 | 115 | 41 | 1 | 3 | 114 | 4 | 118 | 2.85e-45 | 142 |
| MS.gene012931.t1 | AT1G20065 | 62.069 | 58 | 22 | 0 | 3 | 60 | 4 | 61 | 9.71e-20 | 76.6 |
| MS.gene012931.t1 | AT1G20065 | 62.069 | 58 | 22 | 0 | 3 | 60 | 4 | 61 | 9.71e-20 | 76.6 |
| MS.gene012931.t1 | AT1G20065 | 62.069 | 58 | 22 | 0 | 3 | 60 | 4 | 61 | 9.71e-20 | 76.6 |
| MS.gene012931.t1 | AT1G20065 | 62.069 | 58 | 22 | 0 | 3 | 60 | 4 | 61 | 9.71e-20 | 76.6 |
| MS.gene012931.t1 | AT1G20065 | 56.000 | 50 | 22 | 0 | 3 | 52 | 4 | 53 | 8.82e-13 | 59.7 |
Find 21 sgRNAs with CRISPR-Local
Find 112 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAATACCTGTCTGGTATAAA+AGG | 0.277991 | 3.4:-90041898 | None:intergenic |
| CAAAACCAAGTGTAACATAA+AGG | 0.353007 | 3.4:+90040178 | MS.gene012931:CDS |
| ACAGAAGGTGCAATTGTCTA+AGG | 0.372837 | 3.4:+90041755 | MS.gene012931:CDS |
| CAGATGAGATCGGCGGATGA+AGG | 0.477672 | 3.4:+90042004 | MS.gene012931:intron |
| AAAACCAAGTGTAACATAAA+GGG | 0.477760 | 3.4:+90040179 | MS.gene012931:CDS |
| GAAGTCTAGTAATACCTGTC+TGG | 0.505310 | 3.4:-90041907 | None:intergenic |
| TAGGATATCAGAAGAGAACT+TGG | 0.517197 | 3.4:+90041714 | MS.gene012931:intron |
| AGATGAGATCGGCGGATGAA+GGG | 0.551664 | 3.4:+90042005 | MS.gene012931:intron |
| TTGAAACTGATGACCACAGC+GGG | 0.553103 | 3.4:-90042050 | None:intergenic |
| AGTACCCTTTATGTTACACT+TGG | 0.559345 | 3.4:-90040183 | None:intergenic |
| TGATTTAATCGAGGAACAGA+AGG | 0.563158 | 3.4:+90041740 | MS.gene012931:CDS |
| CATAGTGCATAATCAGTTGA+GGG | 0.577852 | 3.4:-90040146 | None:intergenic |
| TTCCTAATTTCAGATGAGAT+CGG | 0.584414 | 3.4:+90041994 | MS.gene012931:intron |
| GCATAGTGCATAATCAGTTG+AGG | 0.598932 | 3.4:-90040147 | None:intergenic |
| AAGTTACACAATTTCTGCTG+AGG | 0.612146 | 3.4:+90041045 | MS.gene012931:CDS |
| ACTTGGAATTGATTTAATCG+AGG | 0.619946 | 3.4:+90041731 | MS.gene012931:CDS |
| CTAATTTCAGATGAGATCGG+CGG | 0.632502 | 3.4:+90041997 | MS.gene012931:intron |
| TTCTACACATGCACCCGCTG+TGG | 0.636738 | 3.4:+90042037 | MS.gene012931:CDS |
| TCTCGACCACGTGAATACAT+TGG | 0.647515 | 3.4:-90040083 | None:intergenic |
| ACAGTGCCAATGTATTCACG+TGG | 0.744012 | 3.4:+90040077 | None:intergenic |
| CTTGAAACTGATGACCACAG+CGG | 0.759797 | 3.4:-90042051 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGAACAAAAAAAAAAAAATT+AGG | + | chr3.4:90040646-90040665 | MS.gene012931:intron | 10.0% |
| !! | ATTCTACAATATATATTTAA+TGG | + | chr3.4:90041784-90041803 | MS.gene012931:intron | 10.0% |
| !!! | CTAATATATTTATTGATTAT+TGG | + | chr3.4:90041662-90041681 | MS.gene012931:intron | 10.0% |
| !! | AAATAAGCTTCAAATTAAAT+TGG | - | chr3.4:90041947-90041966 | None:intergenic | 15.0% |
| !! | AAATTATATCTAAATATGCA+AGG | - | chr3.4:90040909-90040928 | None:intergenic | 15.0% |
| !! | ATATAATCATATCATATGTT+AGG | - | chr3.4:90041121-90041140 | None:intergenic | 15.0% |
| !! | TTTAATATCTGAAAATTACA+AGG | - | chr3.4:90041014-90041033 | None:intergenic | 15.0% |
| !!! | TTGTAATTTTCAGATATTAA+AGG | + | chr3.4:90041013-90041032 | MS.gene012931:intron | 15.0% |
| !! | AAAAAAATTAGGTGACTTTA+TGG | + | chr3.4:90040657-90040676 | MS.gene012931:intron | 20.0% |
| !! | AAAATTAGAAGAAAGAAGTT+TGG | - | chr3.4:90041277-90041296 | None:intergenic | 20.0% |
| !! | AACTAATATATTGTGTGTTT+AGG | + | chr3.4:90040960-90040979 | MS.gene012931:intron | 20.0% |
| !! | ACATATATGGAAATAACATT+AGG | + | chr3.4:90040500-90040519 | MS.gene012931:intron | 20.0% |
| !! | ACATTTATTACAACTTAACA+GGG | - | chr3.4:90040737-90040756 | None:intergenic | 20.0% |
| !! | CTTAAATGGTTTACTAATTT+GGG | + | chr3.4:90040303-90040322 | MS.gene012931:intron | 20.0% |
| !! | TATTAAGATCATTGCTTAAA+TGG | + | chr3.4:90040289-90040308 | MS.gene012931:intron | 20.0% |
| !!! | AAACTTCTTTCTTCTAATTT+TGG | + | chr3.4:90041276-90041295 | MS.gene012931:intron | 20.0% |
| !!! | ATTTTCAGATATTAAAGGAA+AGG | + | chr3.4:90041018-90041037 | MS.gene012931:intron | 20.0% |
| ! | AAAACCAAGTGTAACATAAA+GGG | + | chr3.4:90040179-90040198 | MS.gene012931:CDS | 25.0% |
| ! | AACAAAACATGTGAAAAATG+TGG | + | chr3.4:90041861-90041880 | MS.gene012931:CDS | 25.0% |
| ! | ATAGATGATATATGATACGA+GGG | - | chr3.4:90041354-90041373 | None:intergenic | 25.0% |
| ! | CATTTATTACAACTTAACAG+GGG | - | chr3.4:90040736-90040755 | None:intergenic | 25.0% |
| ! | CTAATGTTATGTTTCTTTAC+AGG | + | chr3.4:90041836-90041855 | MS.gene012931:intron | 25.0% |
| ! | CTTAGAGTGAAAAGAAAATT+TGG | + | chr3.4:90040361-90040380 | MS.gene012931:intron | 25.0% |
| ! | GACATTTATTACAACTTAAC+AGG | - | chr3.4:90040738-90040757 | None:intergenic | 25.0% |
| ! | GCTTAAATGGTTTACTAATT+TGG | + | chr3.4:90040302-90040321 | MS.gene012931:intron | 25.0% |
| ! | TCAATTTCATCTAAATACAC+TGG | - | chr3.4:90041385-90041404 | None:intergenic | 25.0% |
| ! | TGATATCAAATCTTAAACCT+AGG | - | chr3.4:90041594-90041613 | None:intergenic | 25.0% |
| AATTACAAGGACATTCAGTT+AGG | - | chr3.4:90041001-90041020 | None:intergenic | 30.0% | |
| AATTATCCTCATCTACTTTG+TGG | + | chr3.4:90041687-90041706 | MS.gene012931:intron | 30.0% | |
| CAAAACCAAGTGTAACATAA+AGG | + | chr3.4:90040178-90040197 | MS.gene012931:CDS | 30.0% | |
| CATAGATGATATATGATACG+AGG | - | chr3.4:90041355-90041374 | None:intergenic | 30.0% | |
| CTATGATAAACTGTGTTTGT+TGG | + | chr3.4:90041450-90041469 | MS.gene012931:intron | 30.0% | |
| GCAAGGTGTAAAAATTGTTT+AGG | + | chr3.4:90040444-90040463 | MS.gene012931:intron | 30.0% | |
| GGAATCTTCATGATTCTTTA+AGG | + | chr3.4:90040685-90040704 | MS.gene012931:intron | 30.0% | |
| GTAGGTGATCAAATAACTAA+TGG | + | chr3.4:90040387-90040406 | MS.gene012931:intron | 30.0% | |
| GTTTAGGCTGCATAATATAT+AGG | + | chr3.4:90040460-90040479 | MS.gene012931:intron | 30.0% | |
| TAATACCTGTCTGGTATAAA+AGG | - | chr3.4:90041901-90041920 | None:intergenic | 30.0% | |
| TAGATGATATATGATACGAG+GGG | - | chr3.4:90041353-90041372 | None:intergenic | 30.0% | |
| TCATATCATATGTTAGGAGA+TGG | - | chr3.4:90041115-90041134 | None:intergenic | 30.0% | |
| TCTCATCTTGTTAATGCTTA+TGG | + | chr3.4:90040756-90040775 | MS.gene012931:intron | 30.0% | |
| TTCCTAATTTCAGATGAGAT+CGG | + | chr3.4:90041994-90042013 | MS.gene012931:intron | 30.0% | |
| ! | ACTTGGAATTGATTTAATCG+AGG | + | chr3.4:90041731-90041750 | MS.gene012931:CDS | 30.0% |
| ! | TATTGTGACTTTGTTCTAGA+AGG | - | chr3.4:90041330-90041349 | None:intergenic | 30.0% |
| !! | ATTCTGATTTTGAAAGAGCA+AGG | + | chr3.4:90040427-90040446 | MS.gene012931:intron | 30.0% |
| !! | ATTGTGACTTTGTTCTAGAA+GGG | - | chr3.4:90041329-90041348 | None:intergenic | 30.0% |
| !! | GAAAAGAAAATTTGGTGTGT+AGG | + | chr3.4:90040369-90040388 | MS.gene012931:intron | 30.0% |
| !!! | GCTGATAGATTATTTTGAAG+AGG | - | chr3.4:90040224-90040243 | None:intergenic | 30.0% |
| !!! | GCTTTTTGAAATATCAACCT+AGG | + | chr3.4:90041574-90041593 | MS.gene012931:intron | 30.0% |
| AAGTTACACAATTTCTGCTG+AGG | + | chr3.4:90041045-90041064 | MS.gene012931:CDS | 35.0% | |
| ACAGAGCAGATTTAATATGC+AGG | + | chr3.4:90040551-90040570 | MS.gene012931:intron | 35.0% | |
| ACATGTGAAAAATGTGGTCA+TGG | + | chr3.4:90041867-90041886 | MS.gene012931:CDS | 35.0% | |
| AGTACCCTTTATGTTACACT+TGG | - | chr3.4:90040186-90040205 | None:intergenic | 35.0% | |
| ATTCAGTTAGGACGCATAAT+GGG | - | chr3.4:90040989-90041008 | None:intergenic | 35.0% | |
| CAGAGCAGATTTAATATGCA+GGG | + | chr3.4:90040552-90040571 | MS.gene012931:intron | 35.0% | |
| CATAGTGCATAATCAGTTGA+GGG | - | chr3.4:90040149-90040168 | None:intergenic | 35.0% | |
| TAGGATATCAGAAGAGAACT+TGG | + | chr3.4:90041714-90041733 | MS.gene012931:intron | 35.0% | |
| TAGTCTCCAGAAAATAGAGA+AGG | - | chr3.4:90041305-90041324 | None:intergenic | 35.0% | |
| TATATGTGCCTATGTCATAG+TGG | - | chr3.4:90041420-90041439 | None:intergenic | 35.0% | |
| TGATTTAATCGAGGAACAGA+AGG | + | chr3.4:90041740-90041759 | MS.gene012931:CDS | 35.0% | |
| TTTCCATATATGTGTGTAGC+TGG | - | chr3.4:90040493-90040512 | None:intergenic | 35.0% | |
| ! | ATCACTATGTTATGCTGCTT+AGG | + | chr3.4:90041139-90041158 | MS.gene012931:intron | 35.0% |
| ! | GATCTCATCTGAAATTAGGA+AGG | - | chr3.4:90041995-90042014 | None:intergenic | 35.0% |
| ! | TTAGGTGACTTTATGGATTG+AGG | + | chr3.4:90040664-90040683 | MS.gene012931:intron | 35.0% |
| ! | TTATGCTGCTTAGGCTTATA+GGG | + | chr3.4:90041148-90041167 | MS.gene012931:intron | 35.0% |
| !! | ACCTTGATTTGCTTTGTCTT+GGG | + | chr3.4:90041491-90041510 | MS.gene012931:intron | 35.0% |
| !! | TACCTTGATTTGCTTTGTCT+TGG | + | chr3.4:90041490-90041509 | MS.gene012931:intron | 35.0% |
| !! | TTTTGTTCTGTCACTTGTGT+GGG | + | chr3.4:90040108-90040127 | MS.gene012931:CDS | 35.0% |
| !! | TTTTTGTTCTGTCACTTGTG+TGG | + | chr3.4:90040107-90040126 | MS.gene012931:CDS | 35.0% |
| !!! | TTACACTTGGTTTTGCACAA+GGG | - | chr3.4:90040173-90040192 | None:intergenic | 35.0% |
| ACATTAGGTGACATCTCTGA+TGG | + | chr3.4:90040515-90040534 | MS.gene012931:intron | 40.0% | |
| AGATTTAATATGCAGGGACG+CGG | + | chr3.4:90040558-90040577 | MS.gene012931:intron | 40.0% | |
| ATCCTCATCTACTTTGTGGT+TGG | + | chr3.4:90041691-90041710 | MS.gene012931:intron | 40.0% | |
| ATGCCAGCTACACACATATA+TGG | + | chr3.4:90040487-90040506 | MS.gene012931:intron | 40.0% | |
| CATTCAGTTAGGACGCATAA+TGG | - | chr3.4:90040990-90041009 | None:intergenic | 40.0% | |
| CTAATTTCAGATGAGATCGG+CGG | + | chr3.4:90041997-90042016 | MS.gene012931:intron | 40.0% | |
| GAAGTCTAGTAATACCTGTC+TGG | - | chr3.4:90041910-90041929 | None:intergenic | 40.0% | |
| GCATAGTGCATAATCAGTTG+AGG | - | chr3.4:90040150-90040169 | None:intergenic | 40.0% | |
| GGTCTACTGATATGTGCATT+GGG | + | chr3.4:90041169-90041188 | MS.gene012931:intron | 40.0% | |
| GTGACATCTCTGATGGTAAA+AGG | + | chr3.4:90040522-90040541 | MS.gene012931:intron | 40.0% | |
| TACCAACCACAAAGTAGATG+AGG | - | chr3.4:90041696-90041715 | None:intergenic | 40.0% | |
| TCATCTACTTTGTGGTTGGT+AGG | + | chr3.4:90041695-90041714 | MS.gene012931:intron | 40.0% | |
| TCCCAAGACAAAGCAAATCA+AGG | - | chr3.4:90041495-90041514 | None:intergenic | 40.0% | |
| TCTACTGCTTGATACTCCTT+AGG | + | chr3.4:90040850-90040869 | MS.gene012931:intron | 40.0% | |
| TTGATACTCCTTAGGTGTAG+TGG | + | chr3.4:90040858-90040877 | MS.gene012931:intron | 40.0% | |
| ! | ACAGAAGGTGCAATTGTCTA+AGG | + | chr3.4:90041755-90041774 | MS.gene012931:CDS | 40.0% |
| ! | ATGGTTTCGCAAAGTATCTG+TGG | + | chr3.4:90040775-90040794 | MS.gene012931:intron | 40.0% |
| ! | GTTATGCTGCTTAGGCTTAT+AGG | + | chr3.4:90041147-90041166 | MS.gene012931:intron | 40.0% |
| ! | TGGCTTCCTTCTCTATTTTC+TGG | + | chr3.4:90041296-90041315 | MS.gene012931:intron | 40.0% |
| ! | TGGTTTCGCAAAGTATCTGT+GGG | + | chr3.4:90040776-90040795 | MS.gene012931:intron | 40.0% |
| !!! | GTTACACTTGGTTTTGCACA+AGG | - | chr3.4:90040174-90040193 | None:intergenic | 40.0% |
| !!! | TACACTTGGTTTTGCACAAG+GGG | - | chr3.4:90040172-90040191 | None:intergenic | 40.0% |
| ATCCACCTCCACTATGACAT+AGG | + | chr3.4:90041409-90041428 | MS.gene012931:intron | 45.0% | |
| ATGTGCCTATGTCATAGTGG+AGG | - | chr3.4:90041417-90041436 | None:intergenic | 45.0% | |
| CTTGAAACTGATGACCACAG+CGG | - | chr3.4:90042054-90042073 | None:intergenic | 45.0% | |
| GGGTCTACTGATATGTGCAT+TGG | + | chr3.4:90041168-90041187 | MS.gene012931:intron | 45.0% | |
| TTGAAACTGATGACCACAGC+GGG | - | chr3.4:90042053-90042072 | None:intergenic | 45.0% | |
| TTGCTTTGTCTTGGGACTCT+TGG | + | chr3.4:90041499-90041518 | MS.gene012931:intron | 45.0% | |
| TTTGTCTTGGGACTCTTGGT+GGG | + | chr3.4:90041503-90041522 | MS.gene012931:intron | 45.0% | |
| ! | AGCTGCCTTTTATACCAGAC+AGG | + | chr3.4:90041893-90041912 | MS.gene012931:CDS | 45.0% |
| ! | CGCCGATCTCATCTGAAATT+AGG | - | chr3.4:90041999-90042018 | None:intergenic | 45.0% |
| ! | TGTGTCTGAGTTTTGCTGCT+AGG | + | chr3.4:90040617-90040636 | MS.gene012931:intron | 45.0% |
| !! | ACACACTAAGTGCTGAGAAG+TGG | - | chr3.4:90040602-90040621 | None:intergenic | 45.0% |
| !! | ACACTAAGTGCTGAGAAGTG+GGG | - | chr3.4:90040600-90040619 | None:intergenic | 45.0% |
| !! | CACACTAAGTGCTGAGAAGT+GGG | - | chr3.4:90040601-90040620 | None:intergenic | 45.0% |
| AGATGAGATCGGCGGATGAA+GGG | + | chr3.4:90042005-90042024 | MS.gene012931:intron | 50.0% | |
| CTTTGTCTTGGGACTCTTGG+TGG | + | chr3.4:90041502-90041521 | MS.gene012931:intron | 50.0% | |
| GACGTGTACCACTACACCTA+AGG | - | chr3.4:90040869-90040888 | None:intergenic | 50.0% | |
| TGCTGAGAAGTGGGGAATTG+CGG | - | chr3.4:90040592-90040611 | None:intergenic | 50.0% | |
| ! | TGCCTATGTCATAGTGGAGG+TGG | - | chr3.4:90041414-90041433 | None:intergenic | 50.0% |
| CAGATGAGATCGGCGGATGA+AGG | + | chr3.4:90042004-90042023 | MS.gene012931:intron | 55.0% | |
| GCTGAGAAGTGGGGAATTGC+GGG | - | chr3.4:90040591-90040610 | None:intergenic | 55.0% | |
| TTCTACACATGCACCCGCTG+TGG | + | chr3.4:90042037-90042056 | MS.gene012931:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 90040086 | 90042081 | 90040086 | ID=MS.gene012931 |
| chr3.4 | mRNA | 90040086 | 90042081 | 90040086 | ID=MS.gene012931.t1;Parent=MS.gene012931 |
| chr3.4 | exon | 90040086 | 90040200 | 90040086 | ID=MS.gene012931.t1.exon1;Parent=MS.gene012931.t1 |
| chr3.4 | CDS | 90040086 | 90040200 | 90040086 | ID=cds.MS.gene012931.t1;Parent=MS.gene012931.t1 |
| chr3.4 | exon | 90041026 | 90041066 | 90041026 | ID=MS.gene012931.t1.exon2;Parent=MS.gene012931.t1 |
| chr3.4 | CDS | 90041026 | 90041066 | 90041026 | ID=cds.MS.gene012931.t1;Parent=MS.gene012931.t1 |
| chr3.4 | exon | 90041717 | 90041776 | 90041717 | ID=MS.gene012931.t1.exon3;Parent=MS.gene012931.t1 |
| chr3.4 | CDS | 90041717 | 90041776 | 90041717 | ID=cds.MS.gene012931.t1;Parent=MS.gene012931.t1 |
| chr3.4 | exon | 90041858 | 90041914 | 90041858 | ID=MS.gene012931.t1.exon4;Parent=MS.gene012931.t1 |
| chr3.4 | CDS | 90041858 | 90041914 | 90041858 | ID=cds.MS.gene012931.t1;Parent=MS.gene012931.t1 |
| chr3.4 | exon | 90042007 | 90042081 | 90042007 | ID=MS.gene012931.t1.exon5;Parent=MS.gene012931.t1 |
| chr3.4 | CDS | 90042007 | 90042081 | 90042007 | ID=cds.MS.gene012931.t1;Parent=MS.gene012931.t1 |
| Gene Sequence |
| Protein sequence |
