Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene016178.t1 | XP_024631073.1 | 96.6 | 557 | 19 | 0 | 1 | 557 | 7 | 563 | 0.00E+00 | 1087.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene016178.t1 | Q8BVH9 | 43.7 | 254 | 138 | 2 | 10 | 259 | 14 | 266 | 1.4e-51 | 205.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene016178.t1 | A0A396JIH7 | 96.6 | 557 | 19 | 0 | 1 | 557 | 7 | 563 | 0.0e+00 | 1087.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene016178 | MS.gene77822 | PPI |
| MS.gene016178 | MS.gene78481 | PPI |
| MS.gene016178 | MS.gene041598 | PPI |
| MS.gene035362 | MS.gene016178 | PPI |
| MS.gene016178 | MS.gene035363 | PPI |
| MS.gene041599 | MS.gene016178 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene016178.t1 | MTR_1g015770 | 93.794 | 564 | 28 | 1 | 1 | 557 | 7 | 570 | 0.0 | 1070 |
| MS.gene016178.t1 | MTR_1g079800 | 32.963 | 270 | 129 | 7 | 41 | 259 | 53 | 321 | 1.42e-32 | 127 |
| MS.gene016178.t1 | MTR_1g079800 | 32.296 | 257 | 123 | 6 | 53 | 259 | 89 | 344 | 8.24e-29 | 117 |
| MS.gene016178.t1 | MTR_8g056070 | 82.500 | 40 | 7 | 0 | 429 | 468 | 32 | 71 | 1.04e-14 | 70.5 |
| MS.gene016178.t1 | MTR_3g036280 | 78.947 | 38 | 8 | 0 | 431 | 468 | 24 | 61 | 1.23e-13 | 66.2 |
| MS.gene016178.t1 | MTR_3g045720 | 29.252 | 147 | 96 | 4 | 316 | 457 | 35 | 178 | 1.12e-12 | 67.0 |
| MS.gene016178.t1 | MTR_7g032210 | 75.000 | 40 | 10 | 0 | 429 | 468 | 67 | 106 | 1.48e-12 | 65.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene016178.t1 | AT2G26200 | 64.791 | 551 | 187 | 4 | 13 | 557 | 16 | 565 | 0.0 | 728 |
| MS.gene016178.t1 | AT2G26200 | 64.438 | 329 | 106 | 4 | 1 | 320 | 1 | 327 | 4.25e-148 | 430 |
| MS.gene016178.t1 | AT1G54650 | 34.524 | 252 | 140 | 4 | 32 | 259 | 43 | 293 | 1.56e-36 | 138 |
| MS.gene016178.t1 | AT1G54650 | 34.524 | 252 | 140 | 4 | 32 | 259 | 63 | 313 | 2.24e-36 | 138 |
| MS.gene016178.t1 | AT2G26810 | 31.214 | 173 | 108 | 5 | 347 | 514 | 59 | 225 | 3.56e-17 | 82.0 |
| MS.gene016178.t1 | AT2G26810 | 31.214 | 173 | 108 | 5 | 347 | 514 | 59 | 225 | 3.56e-17 | 82.0 |
| MS.gene016178.t1 | AT2G26810 | 38.261 | 115 | 65 | 3 | 347 | 457 | 59 | 171 | 4.84e-15 | 74.7 |
| MS.gene016178.t1 | AT2G26810 | 38.261 | 115 | 65 | 3 | 347 | 457 | 59 | 171 | 4.84e-15 | 74.7 |
| MS.gene016178.t1 | AT2G26810 | 38.261 | 115 | 65 | 3 | 347 | 457 | 59 | 171 | 4.84e-15 | 74.7 |
| MS.gene016178.t1 | AT2G26810 | 38.261 | 115 | 65 | 3 | 347 | 457 | 67 | 179 | 4.98e-15 | 74.7 |
| MS.gene016178.t1 | AT2G26810 | 38.261 | 115 | 65 | 3 | 347 | 457 | 67 | 179 | 4.98e-15 | 74.7 |
| MS.gene016178.t1 | AT1G54650 | 34.906 | 106 | 66 | 1 | 32 | 134 | 63 | 168 | 1.12e-12 | 67.8 |
Find 106 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCAATATTGTTTCCGGAAAA+AGG | 0.103649 | 1.4:-6559889 | MS.gene016178:CDS |
| GCAGATGCATGAAGACTTTA+AGG | 0.160121 | 1.4:-6563650 | MS.gene016178:intron |
| TGTTATTGACAATTGGTTTA+TGG | 0.179744 | 1.4:-6559399 | MS.gene016178:CDS |
| AGGAGGAAAGAAAGTAATTT+TGG | 0.191683 | 1.4:-6564893 | MS.gene016178:CDS |
| CTTTCAGCTGCATCACAATT+TGG | 0.194547 | 1.4:-6559476 | MS.gene016178:CDS |
| ATTTATTCCAAACCAAATTC+TGG | 0.197214 | 1.4:-6566833 | MS.gene016178:CDS |
| ACAAATTTATTCAAAGAAAA+TGG | 0.213631 | 1.4:-6561016 | MS.gene016178:CDS |
| GAAAATCCCAATATTGTTTC+CGG | 0.249931 | 1.4:-6559896 | MS.gene016178:CDS |
| AGACGTTTCAGGCGAATTTC+CGG | 0.250447 | 1.4:+6559427 | None:intergenic |
| AAGGGTGAGACCCCAGAATT+TGG | 0.265459 | 1.4:+6566821 | None:intergenic |
| ATAACACTTCGAGACGTTTC+AGG | 0.273056 | 1.4:+6559416 | None:intergenic |
| AAAGAGAAGCTAAGAAATAT+TGG | 0.275974 | 1.4:-6566687 | MS.gene016178:CDS |
| GATCTCACGGCAATGGCATC+TGG | 0.281647 | 1.4:-6566893 | None:intergenic |
| ATTCGCCTCAGCTTCTTTAC+TGG | 0.282944 | 1.4:+6560773 | None:intergenic |
| AATATGTTTAATGCACTATT+AGG | 0.285579 | 1.4:+6559353 | None:intergenic |
| TTTCAGCTGCATCACAATTT+GGG | 0.310864 | 1.4:-6559475 | MS.gene016178:CDS |
| ATTCTGGGGTCTCACCCTTT+TGG | 0.312133 | 1.4:-6566817 | MS.gene016178:CDS |
| TGATGCTATCACAGGAAATA+TGG | 0.318795 | 1.4:+6564367 | None:intergenic |
| ATAAAGGAGATGAGTGATAG+AGG | 0.329154 | 1.4:-6559665 | MS.gene016178:CDS |
| CATCAAACCAGTTGATTTGC+AGG | 0.330099 | 1.4:+6559958 | None:intergenic |
| TTAATATTCTGCAAAACAAT+AGG | 0.332969 | 1.4:+6563332 | None:intergenic |
| GTGGGAATCGGCCCGATTAA+TGG | 0.336825 | 1.4:-6559933 | MS.gene016178:CDS |
| CTTGATGGTGATAACAATAT+TGG | 0.341012 | 1.4:-6560747 | MS.gene016178:CDS |
| CATCGACACGGCGAAAGATA+TGG | 0.341313 | 1.4:+6559510 | None:intergenic |
| GAAAACTAACCTTGTCTTTG+TGG | 0.362479 | 1.4:+6566647 | None:intergenic |
| TTTATATAGGAGAGATTCTC+TGG | 0.370599 | 1.4:-6561206 | MS.gene016178:intron |
| CTCGAAGTGTTATTGACAAT+TGG | 0.375056 | 1.4:-6559406 | MS.gene016178:CDS |
| CAAGGACGGAAGCCATTAAT+CGG | 0.393239 | 1.4:+6559921 | None:intergenic |
| TTGACATGTCTGAGGGAGTC+GGG | 0.399247 | 1.4:-6560677 | MS.gene016178:CDS |
| TTTATTCCAAACCAAATTCT+GGG | 0.404687 | 1.4:-6566832 | MS.gene016178:CDS |
| CTCACCACGAGCTATTGAAT+TGG | 0.405150 | 1.4:-6564312 | MS.gene016178:CDS |
| GGAGTCGGGGCTGACATGTT+TGG | 0.408447 | 1.4:-6560663 | MS.gene016178:CDS |
| TTTCAAAGACCGGCATTACT+TGG | 0.412980 | 1.4:-6565042 | MS.gene016178:CDS |
| ACGAGCTATTGAATTGGTTA+AGG | 0.421963 | 1.4:-6564306 | MS.gene016178:intron |
| TGAGACCCCAGAATTTGGTT+TGG | 0.422537 | 1.4:+6566826 | None:intergenic |
| ATCTCACGGCAATGGCATCT+GGG | 0.434582 | 1.4:-6566892 | None:intergenic |
| CGCAGCAGCACATATCCATT+TGG | 0.435282 | 1.4:+6563197 | None:intergenic |
| CATGCATAAACAAAAGCATC+TGG | 0.435659 | 1.4:+6564341 | None:intergenic |
| AAGAGAAGCTAAGAAATATT+GGG | 0.435875 | 1.4:-6566686 | MS.gene016178:CDS |
| CAACATACCTGCAAATCAAC+TGG | 0.438434 | 1.4:-6559965 | MS.gene016178:CDS |
| TTGGAGTTAGGGTGTGGCAG+TGG | 0.441103 | 1.4:-6559863 | MS.gene016178:CDS |
| TTTCTCCATTTGCAGCCAAA+TGG | 0.443656 | 1.4:-6563212 | MS.gene016178:intron |
| TGGTTTGATGCTGTGGGAAT+CGG | 0.444115 | 1.4:-6559945 | MS.gene016178:CDS |
| GTTTGCTGGTTTCAGGCGTT+GGG | 0.449293 | 1.4:-6560835 | MS.gene016178:intron |
| GCATTACTTGGATAAAGAAT+GGG | 0.455655 | 1.4:-6565030 | MS.gene016178:CDS |
| GGCATTACTTGGATAAAGAA+TGG | 0.457337 | 1.4:-6565031 | MS.gene016178:CDS |
| ATTATGGAGATCAACCTTAG+AGG | 0.457883 | 1.4:-6560025 | MS.gene016178:CDS |
| AGCTTCTTTACTGGAAGAGT+TGG | 0.466430 | 1.4:+6560782 | None:intergenic |
| AGGGTAGGTACCTCTCCAAA+AGG | 0.473644 | 1.4:+6566802 | None:intergenic |
| GCTGAGGCGAATCATCTTGA+TGG | 0.475133 | 1.4:-6560762 | MS.gene016178:CDS |
| TTTACTGAACAGGTTGGGTG+TGG | 0.479098 | 1.4:-6564401 | MS.gene016178:intron |
| CTGCTGCGAGACTATGCTAC+TGG | 0.479610 | 1.4:-6563182 | MS.gene016178:CDS |
| CTTGCCTTCCGACGAGTATG+AGG | 0.495769 | 1.4:-6560637 | MS.gene016178:intron |
| TTCTGTTGATATTGTTACAA+TGG | 0.496744 | 1.4:-6563560 | MS.gene016178:intron |
| AGATCACATAGAAAGCATAA+AGG | 0.499104 | 1.4:-6559681 | MS.gene016178:CDS |
| GAGTTAGGGTGTGGCAGTGG+TGG | 0.504655 | 1.4:-6559860 | MS.gene016178:CDS |
| TGGAGATCAACCTTAGAGGC+TGG | 0.507549 | 1.4:-6560021 | MS.gene016178:CDS |
| GAACAGGTTGGGTGTGGAGC+TGG | 0.509594 | 1.4:-6564395 | MS.gene016178:intron |
| CAGCGACAAAAGCGTGAACA+TGG | 0.510092 | 1.4:+6563623 | None:intergenic |
| TATGTTATCTGCAGTGTCCC+CGG | 0.526619 | 1.4:-6563363 | MS.gene016178:CDS |
| ATTTGCCACTTCAAACTCAA+AGG | 0.532302 | 1.4:-6566862 | MS.gene016178:CDS |
| GTAGTTAGTTTAGTCAGCAA+TGG | 0.533457 | 1.4:+6559728 | None:intergenic |
| CTGAAACACAAAATACAGCT+TGG | 0.541542 | 1.4:+6560811 | None:intergenic |
| AATCTCTTTGCACAAATCAT+CGG | 0.542409 | 1.4:+6563591 | None:intergenic |
| TGCAACCTTTGAGTTTGAAG+TGG | 0.544850 | 1.4:+6566857 | None:intergenic |
| AAGAAGTTGAGAACCGCTCA+AGG | 0.548248 | 1.4:-6560961 | MS.gene016178:CDS |
| AATCAACTGGTTTGATGCTG+TGG | 0.549620 | 1.4:-6559952 | MS.gene016178:CDS |
| AGGGTATTGGAGTTAGGGTG+TGG | 0.549969 | 1.4:-6559869 | MS.gene016178:CDS |
| GTAACAATATCAACAGAAGA+TGG | 0.550718 | 1.4:+6563565 | None:intergenic |
| CAGTTGATTTGCAGGTATGT+TGG | 0.552753 | 1.4:+6559966 | None:intergenic |
| AAACATATTGCTCTTCTGCA+TGG | 0.557086 | 1.4:-6559339 | MS.gene016178:CDS |
| ATTCTTTATCCAAGTAATGC+CGG | 0.560199 | 1.4:+6565033 | None:intergenic |
| ATCTGTAGCAACTACTCGAT+CGG | 0.561693 | 1.4:+6559808 | None:intergenic |
| TGCTACTGGAGACCTTGCTC+AGG | 0.567273 | 1.4:-6563168 | MS.gene016178:intron |
| TTAGTAGACAAGTGGCCTAC+CGG | 0.568314 | 1.4:-6559446 | MS.gene016178:CDS |
| CAAGGGAGTTGATAATGAAA+CGG | 0.570171 | 1.4:-6560943 | MS.gene016178:intron |
| TCTGGATATGATGCTATCAC+AGG | 0.575758 | 1.4:+6564359 | None:intergenic |
| ATTGACATGTCTGAGGGAGT+CGG | 0.576361 | 1.4:-6560678 | MS.gene016178:CDS |
| AAGGACGGAAGCCATTAATC+GGG | 0.578586 | 1.4:+6559922 | None:intergenic |
| TAAAGGAGATGAGTGATAGA+GGG | 0.579905 | 1.4:-6559664 | MS.gene016178:CDS |
| GGGTAGGTACCTCTCCAAAA+GGG | 0.580724 | 1.4:+6566803 | None:intergenic |
| CTCTTCCAGTAAAGAAGCTG+AGG | 0.582410 | 1.4:-6560778 | MS.gene016178:CDS |
| TCATTATCAACTCCCTTGAG+CGG | 0.584655 | 1.4:+6560948 | None:intergenic |
| GGTTTATATTAGTAGACAAG+TGG | 0.593320 | 1.4:-6559454 | MS.gene016178:CDS |
| AGAGGGTTCAATGTCATCAT+CGG | 0.597470 | 1.4:-6559647 | MS.gene016178:CDS |
| GATCGAGTAGTTGCTACAGA+TGG | 0.598212 | 1.4:-6559806 | MS.gene016178:CDS |
| ATATGGCAAAGAATAAGTAC+AGG | 0.601647 | 1.4:+6559527 | None:intergenic |
| CTCTGTAATTGACATGTCTG+AGG | 0.603915 | 1.4:-6560685 | MS.gene016178:CDS |
| ATCAACTGGTTTGATGCTGT+GGG | 0.608480 | 1.4:-6559951 | MS.gene016178:CDS |
| TTATTCCAAACCAAATTCTG+GGG | 0.617390 | 1.4:-6566831 | MS.gene016178:CDS |
| TGACATGTCTGAGGGAGTCG+GGG | 0.622122 | 1.4:-6560676 | MS.gene016178:CDS |
| ATAGGAGAGATTCTCTGGCA+AGG | 0.623934 | 1.4:-6561201 | MS.gene016178:intron |
| TCTCACGGCAATGGCATCTG+GGG | 0.623959 | 1.4:-6566891 | None:intergenic |
| CAACAAAATCAACCTGAGCA+AGG | 0.630816 | 1.4:+6563156 | None:intergenic |
| CTACAAACACCACAAAGACA+AGG | 0.634893 | 1.4:-6566656 | MS.gene016178:intron |
| CTTACCTCATACTCGTCGGA+AGG | 0.644138 | 1.4:+6560633 | None:intergenic |
| AGAAGTTGAGAACCGCTCAA+GGG | 0.645207 | 1.4:-6560960 | MS.gene016178:CDS |
| GTTTCAGGCGAATTTCCGGT+AGG | 0.649486 | 1.4:+6559431 | None:intergenic |
| TGGTGATAACAATATTGGCA+CGG | 0.662682 | 1.4:-6560742 | MS.gene016178:CDS |
| AAGCCTTACCTCATACTCGT+CGG | 0.662998 | 1.4:+6560629 | None:intergenic |
| AGCGACAAAAGCGTGAACAT+GGG | 0.663872 | 1.4:+6563624 | None:intergenic |
| GCAACGTTGGTTCATCGACA+CGG | 0.677877 | 1.4:+6559498 | None:intergenic |
| CATTACTTGGATAAAGAATG+GGG | 0.679169 | 1.4:-6565029 | MS.gene016178:CDS |
| TCTGTAATTGACATGTCTGA+GGG | 0.683888 | 1.4:-6560684 | MS.gene016178:CDS |
| GATGCAGCTGAAAGCAACGT+TGG | 0.684511 | 1.4:+6559485 | None:intergenic |
| TTAACCAATTCAATAGCTCG+TGG | 0.686527 | 1.4:+6564308 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 6559334 | 6566903 | 6559334 | ID=MS.gene016178 |
| chr1.4 | mRNA | 6559334 | 6566903 | 6559334 | ID=MS.gene016178.t1;Parent=MS.gene016178 |
| chr1.4 | exon | 6566813 | 6566903 | 6566813 | ID=MS.gene016178.t1.exon1;Parent=MS.gene016178.t1 |
| chr1.4 | CDS | 6566813 | 6566903 | 6566813 | ID=cds.MS.gene016178.t1;Parent=MS.gene016178.t1 |
| chr1.4 | exon | 6566657 | 6566718 | 6566657 | ID=MS.gene016178.t1.exon2;Parent=MS.gene016178.t1 |
| chr1.4 | CDS | 6566657 | 6566718 | 6566657 | ID=cds.MS.gene016178.t1;Parent=MS.gene016178.t1 |
| chr1.4 | exon | 6565016 | 6565066 | 6565016 | ID=MS.gene016178.t1.exon3;Parent=MS.gene016178.t1 |
| chr1.4 | CDS | 6565016 | 6565066 | 6565016 | ID=cds.MS.gene016178.t1;Parent=MS.gene016178.t1 |
| chr1.4 | exon | 6564891 | 6564917 | 6564891 | ID=MS.gene016178.t1.exon4;Parent=MS.gene016178.t1 |
| chr1.4 | CDS | 6564891 | 6564917 | 6564891 | ID=cds.MS.gene016178.t1;Parent=MS.gene016178.t1 |
| chr1.4 | exon | 6564307 | 6564411 | 6564307 | ID=MS.gene016178.t1.exon5;Parent=MS.gene016178.t1 |
| chr1.4 | CDS | 6564307 | 6564411 | 6564307 | ID=cds.MS.gene016178.t1;Parent=MS.gene016178.t1 |
| chr1.4 | exon | 6563561 | 6563668 | 6563561 | ID=MS.gene016178.t1.exon6;Parent=MS.gene016178.t1 |
| chr1.4 | CDS | 6563561 | 6563668 | 6563561 | ID=cds.MS.gene016178.t1;Parent=MS.gene016178.t1 |
| chr1.4 | exon | 6563319 | 6563390 | 6563319 | ID=MS.gene016178.t1.exon7;Parent=MS.gene016178.t1 |
| chr1.4 | CDS | 6563319 | 6563390 | 6563319 | ID=cds.MS.gene016178.t1;Parent=MS.gene016178.t1 |
| chr1.4 | exon | 6563169 | 6563219 | 6563169 | ID=MS.gene016178.t1.exon8;Parent=MS.gene016178.t1 |
| chr1.4 | CDS | 6563169 | 6563219 | 6563169 | ID=cds.MS.gene016178.t1;Parent=MS.gene016178.t1 |
| chr1.4 | exon | 6561157 | 6561219 | 6561157 | ID=MS.gene016178.t1.exon9;Parent=MS.gene016178.t1 |
| chr1.4 | CDS | 6561157 | 6561219 | 6561157 | ID=cds.MS.gene016178.t1;Parent=MS.gene016178.t1 |
| chr1.4 | exon | 6560944 | 6561068 | 6560944 | ID=MS.gene016178.t1.exon10;Parent=MS.gene016178.t1 |
| chr1.4 | CDS | 6560944 | 6561068 | 6560944 | ID=cds.MS.gene016178.t1;Parent=MS.gene016178.t1 |
| chr1.4 | exon | 6560638 | 6560842 | 6560638 | ID=MS.gene016178.t1.exon11;Parent=MS.gene016178.t1 |
| chr1.4 | CDS | 6560638 | 6560842 | 6560638 | ID=cds.MS.gene016178.t1;Parent=MS.gene016178.t1 |
| chr1.4 | exon | 6559334 | 6560047 | 6559334 | ID=MS.gene016178.t1.exon12;Parent=MS.gene016178.t1 |
| chr1.4 | CDS | 6559334 | 6560047 | 6559334 | ID=cds.MS.gene016178.t1;Parent=MS.gene016178.t1 |
| Gene Sequence |
| Protein sequence |
