AlfalfaGEDB | MS.gene041598

M. sativa cultivar XinJiangDaYe / MS.gene041598

KEGG: K10418
GO: GO:0005575 GO:0005875 GO:0007017 GO:0008150 GO:0009987 GO:0030286 GO:0032991 GO:1902494
PFAM: PF01221.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene041598.t1	XP_003609697.1	98.1	103	2	0	1	103	1	103	6.30E-47	196.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene041598.t1	Q3MHR3	62.7	59	22	0	45	103	4	62	3.8e-16	85.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene041598.t1	G7JLJ9	98.1	103	2	0	1	103	1	103	4.6e-47	196.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene25631	MS.gene041598	PPI
MS.gene024860	MS.gene041598	PPI
MS.gene004435	MS.gene041598	PPI
MS.gene35155	MS.gene041598	PPI
MS.gene77163	MS.gene041598	PPI
MS.gene071364	MS.gene041598	PPI
MS.gene043645	MS.gene041598	PPI
MS.gene09540	MS.gene041598	PPI
MS.gene96225	MS.gene041598	PPI
MS.gene49473	MS.gene041598	PPI
MS.gene043821	MS.gene041598	PPI
MS.gene21834	MS.gene041598	PPI
MS.gene25632	MS.gene041598	PPI
MS.gene043818	MS.gene041598	PPI
MS.gene064353	MS.gene041598	PPI
MS.gene028302	MS.gene041598	PPI
MS.gene77160	MS.gene041598	PPI
MS.gene95803	MS.gene041598	PPI
MS.gene035825	MS.gene041598	PPI
MS.gene73093	MS.gene041598	PPI
MS.gene016178	MS.gene041598	PPI
MS.gene043643	MS.gene041598	PPI
MS.gene81099	MS.gene041598	PPI
MS.gene035826	MS.gene041598	PPI
MS.gene002746	MS.gene041598	PPI
MS.gene33030	MS.gene041598	PPI
MS.gene070829	MS.gene041598	PPI
MS.gene064351	MS.gene041598	PPI
MS.gene035828	MS.gene041598	PPI
MS.gene34372	MS.gene041598	PPI
MS.gene023152	MS.gene041598	PPI
MS.gene20245	MS.gene041598	PPI
MS.gene25633	MS.gene041598	PPI
MS.gene001031	MS.gene041598	PPI
MS.gene36490	MS.gene041598	PPI
MS.gene015202	MS.gene041598	PPI
MS.gene99872	MS.gene041598	PPI
MS.gene070831	MS.gene041598	PPI
MS.gene043595	MS.gene041598	PPI
MS.gene071366	MS.gene041598	PPI
MS.gene09543	MS.gene041598	PPI
MS.gene051574	MS.gene041598	PPI
MS.gene070946	MS.gene041598	PPI
MS.gene29908	MS.gene041598	PPI
MS.gene043593	MS.gene041598	PPI
MS.gene015206	MS.gene041598	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene041598.t1	MTR_4g120150	98.058	103	2	0	1	103	1	103	1.79e-71	209
MS.gene041598.t1	MTR_8g464690	48.333	60	29	1	46	103	5	64	4.14e-15	65.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene041598.t1	AT4G15930	58.252	103	36	3	1	103	1	96	4.74e-35	115
MS.gene041598.t1	AT4G15930	58.252	103	36	3	1	103	1	96	5.75e-35	116

Find 24 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTCTTCGTTGCGGGTGTTGC+AGG	0.303894	8.4:+11691297	None:intergenic
ATAATGACCTTCTTCGTTGC+GGG	0.323218	8.4:+11691288	None:intergenic
TGTATTGTTGGTCGCAATTT+CGG	0.332097	8.4:-11691017	None:intergenic
CCTACTTGGCATTGTATTGT+TGG	0.395219	8.4:-11691029	MS.gene041598:CDS
ACAAGAGGCATGGTCCTACT+TGG	0.447517	8.4:-11691043	MS.gene041598:CDS
AAGAAGCACAGCGCAGGTGG+AGG	0.466246	8.4:-11691396	MS.gene041598:CDS
GCGAAGAAGCACAGCGCAGG+TGG	0.479599	8.4:-11691399	MS.gene041598:CDS
GAGGCGAAGAAGCACAGCGC+AGG	0.496399	8.4:-11691402	MS.gene041598:CDS
AGGTGGAGGAGCATCTCTAA+TGG	0.510524	8.4:-11691382	MS.gene041598:CDS
TGCAACACCCGCAACGAAGA+AGG	0.520232	8.4:-11691295	MS.gene041598:CDS
TTGCATGTCAGGGAGCATAT+CGG	0.526660	8.4:+11691257	None:intergenic
TGTTGCAGAGACTATAAAGA+AGG	0.528953	8.4:-11691072	MS.gene041598:CDS
TGCGGGTGTTGCAGGAGAAG+CGG	0.546280	8.4:+11691305	None:intergenic
TATGCTCCCTGACATGCAAA+AGG	0.547026	8.4:-11691253	MS.gene041598:CDS
GATAATGACCTTCTTCGTTG+CGG	0.566862	8.4:+11691287	None:intergenic
GGAGAAGCGGATGCAGAGAG+TGG	0.576599	8.4:+11691318	None:intergenic
CCAACAATACAATGCCAAGT+AGG	0.596782	8.4:+11691029	None:intergenic
AATTGAATTAGGCATTTGAG+AGG	0.599018	8.4:-11691112	MS.gene041598:intron
TAGGTAAAGAAAGATGAGCG+AGG	0.624160	8.4:-11691424	None:intergenic
AAGGAGTTTGACAAGAGGCA+TGG	0.631606	8.4:-11691053	MS.gene041598:CDS
GAGAGTGGAGATGTAACAAG+TGG	0.663410	8.4:+11691333	None:intergenic
AACAACGACAGCGATATCAA+CGG	0.685652	8.4:+11691227	None:intergenic
TAAAGAAGGAGTTTGACAAG+AGG	0.686443	8.4:-11691058	MS.gene041598:CDS
GTAAAGAAAGATGAGCGAGG+AGG	0.700815	8.4:-11691421	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAATTAGAAAAAATTGAAGA+AGG	+	chr8.4:11691262-11691281	None:intergenic	15.0%
!!!	TATTGTTTTGAAATTGAATT+AGG	-	chr8.4:11691306-11691325	MS.gene041598:CDS	15.0%
	AATTGAATTAGGCATTTGAG+AGG	-	chr8.4:11691317-11691336	MS.gene041598:CDS	30.0%
	TAAAGAAGGAGTTTGACAAG+AGG	-	chr8.4:11691371-11691390	MS.gene041598:CDS	35.0%
	TGTTGCAGAGACTATAAAGA+AGG	-	chr8.4:11691357-11691376	MS.gene041598:CDS	35.0%
!	AACAAGTGGTTTTCGATCTT+CGG	+	chr8.4:11691085-11691104	None:intergenic	35.0%
	AACAACGACAGCGATATCAA+CGG	+	chr8.4:11691205-11691224	None:intergenic	40.0%
	ATAATGACCTTCTTCGTTGC+GGG	+	chr8.4:11691144-11691163	None:intergenic	40.0%
	CCAACAATACAATGCCAAGT+AGG	+	chr8.4:11691403-11691422	None:intergenic	40.0%
	CCTACTTGGCATTGTATTGT+TGG	-	chr8.4:11691400-11691419	MS.gene041598:CDS	40.0%
	GATAATGACCTTCTTCGTTG+CGG	+	chr8.4:11691145-11691164	None:intergenic	40.0%
	AAGGAGTTTGACAAGAGGCA+TGG	-	chr8.4:11691376-11691395	MS.gene041598:CDS	45.0%
	GAGAGTGGAGATGTAACAAG+TGG	+	chr8.4:11691099-11691118	None:intergenic	45.0%
	TATGCTCCCTGACATGCAAA+AGG	-	chr8.4:11691176-11691195	MS.gene041598:intron	45.0%
!	TGGTTTTCGATCTTCGGTGA+GGG	+	chr8.4:11691079-11691098	None:intergenic	45.0%
!	TTGCATGTCAGGGAGCATAT+CGG	+	chr8.4:11691175-11691194	None:intergenic	45.0%
!	ACAAGAGGCATGGTCCTACT+TGG	-	chr8.4:11691386-11691405	MS.gene041598:CDS	50.0%
!	ACGGCTTCCTTTTGCATGTC+AGG	+	chr8.4:11691186-11691205	None:intergenic	50.0%
!	CGGCTTCCTTTTGCATGTCA+GGG	+	chr8.4:11691185-11691204	None:intergenic	50.0%
!	GTGGTTTTCGATCTTCGGTG+AGG	+	chr8.4:11691080-11691099	None:intergenic	50.0%
!!	AGGTGGAGGAGCATCTCTAA+TGG	-	chr8.4:11691047-11691066	MS.gene041598:CDS	50.0%
	TGCAACACCCGCAACGAAGA+AGG	-	chr8.4:11691134-11691153	MS.gene041598:intron	55.0%
!	TTCTTCGTTGCGGGTGTTGC+AGG	+	chr8.4:11691135-11691154	None:intergenic	55.0%
	GGAGAAGCGGATGCAGAGAG+TGG	+	chr8.4:11691114-11691133	None:intergenic	60.0%
!	AAGAAGCACAGCGCAGGTGG+AGG	-	chr8.4:11691033-11691052	MS.gene041598:CDS	60.0%
!	TGCGGGTGTTGCAGGAGAAG+CGG	+	chr8.4:11691127-11691146	None:intergenic	60.0%
!	GAGGCGAAGAAGCACAGCGC+AGG	-	chr8.4:11691027-11691046	MS.gene041598:CDS	65.0%
!	GCGAAGAAGCACAGCGCAGG+TGG	-	chr8.4:11691030-11691049	MS.gene041598:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.4	gene	11691018	11691433	11691018	ID=MS.gene041598
chr8.4	mRNA	11691018	11691433	11691018	ID=MS.gene041598.t1;Parent=MS.gene041598
chr8.4	exon	11691230	11691433	11691230	ID=MS.gene041598.t1.exon1;Parent=MS.gene041598.t1
chr8.4	CDS	11691230	11691433	11691230	ID=cds.MS.gene041598.t1;Parent=MS.gene041598.t1
chr8.4	exon	11691018	11691123	11691018	ID=MS.gene041598.t1.exon2;Parent=MS.gene041598.t1
chr8.4	CDS	11691018	11691123	11691018	ID=cds.MS.gene041598.t1;Parent=MS.gene041598.t1

Gene Sequence

>MS.gene041598

ATGAGCGAGGAGGCGAAGAAGCACAGCGCAGGTGGAGGAGCATCTCTAATGGCGAAATCCCTCACCGAAGATCGAAAACCACTTGTTACATCTCCACTCTCTGCATCCGCTTCTCCTGCAACACCCGCAACGAAGAAGGTCATTATCAAGAGTGCCGATATGCTCCCTGACATGCAAAAGGAAGCCGTTGATATCGCTGTCGTTGTTAGTCTCTCTTACTCTTCAACATTCACTTCTTCTTCCTTCTTCAATTTTTTCTAATTTTCATTTTTTTTTATTTTTTGCTAATATTGTTTTGAAATTGAATTAGGCATTTGAGAGGCACAGCGTAGAGAAAGATGTTGCAGAGACTATAAAGAAGGAGTTTGACAAGAGGCATGGTCCTACTTGGCATTGTATTGTTGGTCGCAATTTCG

Protein sequence

>MS.gene041598.t1

MSEEAKKHSAGGGASLMAKSLTEDRKPLVTSPLSASASPATPATKKVIIKSADMLPDMQKEAVDIAVVAFERHSVEKDVAETIKKEFDKRHGPTWHCIVGRNF