Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene035363.t1 | XP_003609697.1 | 96.2 | 106 | 4 | 0 | 1 | 106 | 1 | 106 | 1.20E-47 | 199.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene035363.t1 | Q3MHR3 | 61.3 | 62 | 24 | 0 | 45 | 106 | 4 | 65 | 7.2e-17 | 88.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene035363.t1 | G7JLJ9 | 96.2 | 106 | 4 | 0 | 1 | 106 | 1 | 106 | 8.7e-48 | 199.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene035363.t1 | MTR_4g120150 | 96.226 | 106 | 4 | 0 | 1 | 106 | 1 | 106 | 5.80e-72 | 213 |
| MS.gene035363.t1 | MTR_8g464690 | 49.206 | 63 | 30 | 1 | 46 | 106 | 5 | 67 | 7.25e-16 | 69.7 |
| MS.gene035363.t1 | MTR_4g107350 | 53.448 | 58 | 25 | 1 | 51 | 106 | 220 | 277 | 2.09e-11 | 61.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene035363.t1 | AT4G15930 | 57.547 | 106 | 38 | 3 | 1 | 106 | 1 | 99 | 1.79e-35 | 120 |
| MS.gene035363.t1 | AT4G15930 | 58.654 | 104 | 36 | 3 | 1 | 104 | 1 | 97 | 4.82e-35 | 118 |
Find 0 sgRNAs with CRISPR-Local
Find 138 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATGTAAAAAATTAAATTCTA+AGG | + | 32571:9440-9459 | MS.gene035363:intron | 10.0% |
| !! | AAATTAGAAAAAATTGAAGA+AGG | - | 32571:9043-9062 | MS.gene035363:intergenic | 15.0% |
| !! | ATAGATTATAGATCTAATTA+AGG | - | 32571:9232-9251 | MS.gene035363:intergenic | 15.0% |
| !! | ATCAAAAGAAAATATATGTT+TGG | - | 32571:10814-10833 | MS.gene035363:intergenic | 15.0% |
| !! | GAAAGATATAAATATTTGAT+GGG | + | 32571:10882-10901 | MS.gene035363:intron | 15.0% |
| !! | TAGATTATAGATCTAATTAA+GGG | - | 32571:9231-9250 | MS.gene035363:intergenic | 15.0% |
| !! | TATAAGTTTGATATTATGAA+CGG | + | 32571:10548-10567 | MS.gene035363:intron | 15.0% |
| !! | TGAAAGATATAAATATTTGA+TGG | + | 32571:10881-10900 | MS.gene035363:intron | 15.0% |
| !!! | CTAACAACAATTTTAAAAAA+TGG | - | 32571:9502-9521 | MS.gene035363:intergenic | 15.0% |
| !!! | TATTGTTTTGAAATTGAATT+AGG | + | 32571:9087-9106 | MS.gene035363:intron | 15.0% |
| !!! | TTTTTTTTTTTTTTGTGATG+TGG | + | 32571:10357-10376 | MS.gene035363:intron | 15.0% |
| !! | AGATTATAGATCTAATTAAG+GGG | - | 32571:9230-9249 | MS.gene035363:intergenic | 20.0% |
| !! | ATTCTCATAAAGATAGAATT+GGG | - | 32571:10019-10038 | MS.gene035363:intergenic | 20.0% |
| !! | GTAGTAAATAGAAAAAATCT+AGG | - | 32571:9360-9379 | MS.gene035363:intergenic | 20.0% |
| !! | TATTCTCATAAAGATAGAAT+TGG | - | 32571:10020-10039 | MS.gene035363:intergenic | 20.0% |
| !! | TGACTTATTCATAATACAAT+CGG | - | 32571:10597-10616 | MS.gene035363:intergenic | 20.0% |
| !!! | AGTATTTAGTTTGTTAACTT+TGG | + | 32571:9825-9844 | MS.gene035363:intron | 20.0% |
| !!! | GTGTTTTATAGATTGAATTT+TGG | + | 32571:9560-9579 | MS.gene035363:intron | 20.0% |
| ! | AACATTTACACAATTTAGCA+TGG | - | 32571:10058-10077 | MS.gene035363:intergenic | 25.0% |
| ! | CAATCTATAAAACACATACA+CGG | - | 32571:9555-9574 | MS.gene035363:intergenic | 25.0% |
| ! | CATAACTATGAGTTAGAAAT+TGG | - | 32571:9327-9346 | MS.gene035363:intergenic | 25.0% |
| ! | TAACACTATTAAAGTTCTTC+AGG | - | 32571:9901-9920 | MS.gene035363:intergenic | 25.0% |
| !! | ATATCACTTCTAGAAAAGAA+GGG | + | 32571:10743-10762 | MS.gene035363:intron | 25.0% |
| !! | TATATCACTTCTAGAAAAGA+AGG | + | 32571:10742-10761 | MS.gene035363:intron | 25.0% |
| !! | TTACTACTGTTTTCTTATTG+TGG | + | 32571:9373-9392 | MS.gene035363:intron | 25.0% |
| !! | TTATGTTTTCTTCTCTCATA+TGG | + | 32571:10136-10155 | MS.gene035363:intron | 25.0% |
| !!! | AAATACTGTTTTTTCACTGA+GGG | - | 32571:9812-9831 | MS.gene035363:intergenic | 25.0% |
| !!! | AAATATTTGATGGGTTTGAT+AGG | + | 32571:10891-10910 | MS.gene035363:intron | 25.0% |
| !!! | AATATTTGATGGGTTTGATA+GGG | + | 32571:10892-10911 | MS.gene035363:intron | 25.0% |
| !!! | TAAATACTGTTTTTTCACTG+AGG | - | 32571:9813-9832 | MS.gene035363:intergenic | 25.0% |
| !!! | TTAGTTTGTTAACTTTGGAT+CGG | + | 32571:9830-9849 | MS.gene035363:intron | 25.0% |
| !!! | TTTTTTTTGTGATGTGGAAA+AGG | + | 32571:10363-10382 | MS.gene035363:intron | 25.0% |
| AAACAGGTATGTCGTTAAAT+TGG | - | 32571:9783-9802 | MS.gene035362:intergenic | 30.0% | |
| AAAGAACACAGAATACAAAG+TGG | - | 32571:9698-9717 | MS.gene035362:intergenic | 30.0% | |
| AATTGAATTAGGCATTTGAG+AGG | + | 32571:9098-9117 | MS.gene035363:intron | 30.0% | |
| AATTTAACGACATACCTGTT+TGG | + | 32571:9782-9801 | MS.gene035363:intron | 30.0% | |
| ATCTTTCATTCTACACTTCA+TGG | - | 32571:10869-10888 | MS.gene035363:intergenic | 30.0% | |
| TGTGTAGTTAACAAAATGGA+AGG | + | 32571:9396-9415 | MS.gene035363:intron | 30.0% | |
| ! | CTGTGAACAGAAAAATGTTT+TGG | - | 32571:9954-9973 | MS.gene035363:intergenic | 30.0% |
| !!! | AATACTGTTTTTTCACTGAG+GGG | - | 32571:9811-9830 | MS.gene035363:intergenic | 30.0% |
| AAGATGTTGGATGTCGAAAA+GGG | - | 32571:9990-10009 | MS.gene035362:intergenic | 35.0% | |
| AATTGGGAACAACAAGATGT+TGG | - | 32571:10003-10022 | MS.gene035363:intergenic | 35.0% | |
| AGATGAGTAATTCTTGAGTG+GGG | + | 32571:9615-9634 | MS.gene035363:intron | 35.0% | |
| CAATTGCCATAAATTCCCTA+AGG | - | 32571:10842-10861 | MS.gene035363:intergenic | 35.0% | |
| GACACACACAAAATGTATTG+AGG | - | 32571:10646-10665 | MS.gene035363:intergenic | 35.0% | |
| GAGATGAGTAATTCTTGAGT+GGG | + | 32571:9614-9633 | MS.gene035363:intron | 35.0% | |
| GTGGTGTGTAGTTAACAAAA+TGG | + | 32571:9392-9411 | MS.gene035363:intron | 35.0% | |
| GTTCCAGTTTCAAAATACCA+GGG | - | 32571:10684-10703 | MS.gene035363:intergenic | 35.0% | |
| TAAAGAAGGAGTTTGACAAG+AGG | + | 32571:9152-9171 | MS.gene035363:CDS | 35.0% | |
| TGAAGTCTAATACATCTGAG+AGG | + | 32571:10266-10285 | MS.gene035363:intron | 35.0% | |
| TGTATTCTGTGTTCTTTGCA+GGG | + | 32571:9701-9720 | MS.gene035363:intron | 35.0% | |
| TGTATTGTTGGTCGCAATTT+CGG | + | 32571:9193-9212 | MS.gene035363:CDS | 35.0% | |
| TGTTGCAGAGACTATAAAGA+AGG | + | 32571:9138-9157 | MS.gene035363:CDS | 35.0% | |
| TTGGAATGATAAACTGACAC+AGG | - | 32571:9764-9783 | MS.gene035363:intergenic | 35.0% | |
| TTGTATTCTGTGTTCTTTGC+AGG | + | 32571:9700-9719 | MS.gene035363:intron | 35.0% | |
| ! | AACAAGTGGTTTTCGATCTT+CGG | - | 32571:8866-8885 | MS.gene035363:intergenic | 35.0% |
| ! | CAGTAAATAACAAAGTGCCA+CGG | - | 32571:10111-10130 | MS.gene035363:intergenic | 35.0% |
| ! | TTCTAGAAAAGAAGGGTCAA+GGG | + | 32571:10750-10769 | MS.gene035363:intron | 35.0% |
| ! | TTCTTTTGATGTCAGCCTTA+GGG | + | 32571:10824-10843 | MS.gene035363:intron | 35.0% |
| ! | TTTCTTTTGATGTCAGCCTT+AGG | + | 32571:10823-10842 | MS.gene035363:intron | 35.0% |
| !! | ATTAAATTCTAAGGCGTGTC+TGG | + | 32571:9449-9468 | MS.gene035363:intron | 35.0% |
| !!! | CTGGTATTTTGAAACTGGAA+CGG | + | 32571:10683-10702 | MS.gene035363:intron | 35.0% |
| !!! | CTTTTCGAGTTATTTCTACC+TGG | + | 32571:9592-9611 | MS.gene035363:intron | 35.0% |
| AACAACGACAGCGATATCAA+CGG | - | 32571:8986-9005 | MS.gene035362:intergenic | 40.0% | |
| ACACAATTTAGCATGGCGTT+TGG | - | 32571:10051-10070 | MS.gene035363:intergenic | 40.0% | |
| AGTCTAATACATCTGAGAGG+AGG | + | 32571:10269-10288 | MS.gene035363:intron | 40.0% | |
| ATAATGACCTTCTTCGTTGC+GGG | - | 32571:8925-8944 | MS.gene035363:intergenic | 40.0% | |
| ATCAAAGCTTGGACTAAGCT+CGG | + | 32571:10949-10968 | MS.gene035363:CDS | 40.0% | |
| CAAGATGTTGGATGTCGAAA+AGG | - | 32571:9991-10010 | MS.gene035363:intergenic | 40.0% | |
| CCAACAATACAATGCCAAGT+AGG | - | 32571:9184-9203 | MS.gene035363:intergenic | 40.0% | |
| CCTACTTGGCATTGTATTGT+TGG | + | 32571:9181-9200 | MS.gene035363:CDS | 40.0% | |
| CGTTCCAGTTTCAAAATACC+AGG | - | 32571:10685-10704 | MS.gene035363:intergenic | 40.0% | |
| CTCAAGAATTACTCATCTCC+AGG | - | 32571:9613-9632 | MS.gene035363:intergenic | 40.0% | |
| GAAACTGGAACGGCTAATAA+CGG | + | 32571:10693-10712 | MS.gene035363:intron | 40.0% | |
| GACAGGAGTAGAAATCACAT+AGG | - | 32571:10483-10502 | MS.gene035363:intergenic | 40.0% | |
| GAGTTTGATCGTAGTGGTAT+AGG | + | 32571:9671-9690 | MS.gene035363:intron | 40.0% | |
| GATAATGACCTTCTTCGTTG+CGG | - | 32571:8926-8945 | MS.gene035363:intergenic | 40.0% | |
| GGAGATGAGTAATTCTTGAG+TGG | + | 32571:9613-9632 | MS.gene035363:intron | 40.0% | |
| GGGATAGAATAGGCTTGATA+GGG | + | 32571:9870-9889 | MS.gene035363:intron | 40.0% | |
| GTATTCTGTGTTCTTTGCAG+GGG | + | 32571:9702-9721 | MS.gene035363:intron | 40.0% | |
| GTCTAATACATCTGAGAGGA+GGG | + | 32571:10270-10289 | MS.gene035363:intron | 40.0% | |
| GTTAAGATGTGCAGAATGAC+AGG | - | 32571:10500-10519 | MS.gene035363:intergenic | 40.0% | |
| TATTCTGTGTTCTTTGCAGG+GGG | + | 32571:9703-9722 | MS.gene035363:intron | 40.0% | |
| TGGGATAGAATAGGCTTGAT+AGG | + | 32571:9869-9888 | MS.gene035363:intron | 40.0% | |
| TGTCAGCCTTAGGGAATTTA+TGG | + | 32571:10833-10852 | MS.gene035363:intron | 40.0% | |
| ! | AATTCTTGAGTGGGGTACTT+AGG | + | 32571:9623-9642 | MS.gene035363:intron | 40.0% |
| ! | AATTTGAGACTCAAGCAGTG+CGG | - | 32571:10449-10468 | MS.gene035363:intergenic | 40.0% |
| ! | ATTCTTGAGTGGGGTACTTA+GGG | + | 32571:9624-9643 | MS.gene035363:intron | 40.0% |
| ! | CTTCTAGAAAAGAAGGGTCA+AGG | + | 32571:10749-10768 | MS.gene035363:intron | 40.0% |
| ! | TTTCAAGTCGAGTTAATCCG+TGG | + | 32571:10091-10110 | MS.gene035363:intron | 40.0% |
| !! | TTTGATGGGTTTGATAGGGA+TGG | + | 32571:10896-10915 | MS.gene035363:intron | 40.0% |
| AAGGAGTTTGACAAGAGGCA+TGG | + | 32571:9157-9176 | MS.gene035363:CDS | 45.0% | |
| CCAACTGAGTTTGATCGTAG+TGG | + | 32571:9665-9684 | MS.gene035363:intron | 45.0% | |
| CCACTACGATCAAACTCAGT+TGG | - | 32571:9668-9687 | MS.gene035363:intergenic | 45.0% | |
| CTATTCCGTGGAAGAGGAAA+AGG | - | 32571:9738-9757 | MS.gene035363:intergenic | 45.0% | |
| GAGAGTGGAGATGTAACAAG+TGG | - | 32571:8880-8899 | MS.gene035363:intergenic | 45.0% | |
| GTGTGTGCTTGGGATAGAAT+AGG | + | 32571:9860-9879 | MS.gene035363:intron | 45.0% | |
| TATGCTCCCTGACATGCAAA+AGG | + | 32571:8957-8976 | MS.gene035363:CDS | 45.0% | |
| TCAAAGCTTGGACTAAGCTC+GGG | + | 32571:10950-10969 | MS.gene035363:CDS | 45.0% | |
| TTAGCATGGCGTTTGGACTA+TGG | - | 32571:10044-10063 | MS.gene035363:intergenic | 45.0% | |
| ! | AGTCCAAGCTTTGATTGTGC+TGG | - | 32571:10944-10963 | MS.gene035363:intergenic | 45.0% |
| ! | ATTTGAGACTCAAGCAGTGC+GGG | - | 32571:10448-10467 | MS.gene035363:intergenic | 45.0% |
| ! | CGGAGTTGTTTGCTGAACTT+AGG | + | 32571:10568-10587 | MS.gene035363:intron | 45.0% |
| ! | GTCCAAGCTTTGATTGTGCT+GGG | - | 32571:10943-10962 | MS.gene035363:intergenic | 45.0% |
| ! | TGGTTTTCGATCTTCGGTGA+GGG | - | 32571:8860-8879 | MS.gene035363:intergenic | 45.0% |
| ! | TTGCATGTCAGGGAGCATAT+CGG | - | 32571:8956-8975 | MS.gene035363:intergenic | 45.0% |
| !!! | AAGCTTAAGTCGCTTTTGCG+TGG | - | 32571:10522-10541 | MS.gene035363:intergenic | 45.0% |
| !!! | GCTCCCTGGTATTTTGAAAC+TGG | + | 32571:10678-10697 | MS.gene035363:intron | 45.0% |
| !! | AAAAAAAAAAAATAGTATAT+TGG | - | 32571:10348-10367 | MS.gene035363:intergenic | 5.0% |
| AGCCCAGCACAATCAAAGCT+TGG | + | 32571:10938-10957 | MS.gene035363:CDS | 50.0% | |
| AGCTCGGGTTTCAGATACCA+TGG | + | 32571:10965-10984 | MS.gene035363:CDS | 50.0% | |
| CGGATTGAGTGTGTGTGCTT+GGG | + | 32571:9850-9869 | MS.gene035363:intron | 50.0% | |
| GTTCTGCTCAGCAATATCGC+AGG | - | 32571:10222-10241 | MS.gene035363:intergenic | 50.0% | |
| TAGGGATGGAGAGACTGCAA+AGG | + | 32571:10910-10929 | MS.gene035363:intron | 50.0% | |
| TCAAAATACCAGGGAGCCAG+AGG | - | 32571:10675-10694 | MS.gene035363:intergenic | 50.0% | |
| TCACTGAGGGGAAACCAAAC+AGG | - | 32571:9799-9818 | MS.gene035363:intergenic | 50.0% | |
| TCGGATTGAGTGTGTGTGCT+TGG | + | 32571:9849-9868 | MS.gene035363:intron | 50.0% | |
| TGTGTGTGTCAAGAGTCCTC+TGG | + | 32571:10656-10675 | MS.gene035363:intron | 50.0% | |
| ! | ACAAGAGGCATGGTCCTACT+TGG | + | 32571:9167-9186 | MS.gene035363:CDS | 50.0% |
| ! | ACGGCTTCCTTTTGCATGTC+AGG | - | 32571:8967-8986 | MS.gene035363:intergenic | 50.0% |
| ! | CGGCTTCCTTTTGCATGTCA+GGG | - | 32571:8966-8985 | MS.gene035363:intergenic | 50.0% |
| ! | GGGGTACTTAGGGATGACAA+TGG | + | 32571:9634-9653 | MS.gene035363:intron | 50.0% |
| ! | GGTGTCCTTTTCCTCTTCCA+CGG | + | 32571:9730-9749 | MS.gene035363:CDS | 50.0% |
| ! | GTGGTTTTCGATCTTCGGTG+AGG | - | 32571:8861-8880 | MS.gene035363:intergenic | 50.0% |
| ! | GTGTTCTTTGCAGGGGGTTT+TGG | + | 32571:9709-9728 | MS.gene035363:intron | 50.0% |
| !! | AGGTGGAGGAGCATCTCTAA+TGG | + | 32571:8828-8847 | MS.gene035363:CDS | 50.0% |
| !! | TGGCTTCTGGAACATCACCA+TGG | - | 32571:10985-11004 | MS.gene035363:intergenic | 50.0% |
| AGGCAGCTATTCCGTGGAAG+AGG | - | 32571:9744-9763 | MS.gene035363:intergenic | 55.0% | |
| TGACACAGGCAGCTATTCCG+TGG | - | 32571:9750-9769 | MS.gene035363:intergenic | 55.0% | |
| TGCAACACCCGCAACGAAGA+AGG | + | 32571:8915-8934 | MS.gene035363:CDS | 55.0% | |
| ! | GGTCAAGGGAGTGTGATCTC+TGG | + | 32571:10764-10783 | MS.gene035363:intron | 55.0% |
| ! | TTCTTCGTTGCGGGTGTTGC+AGG | - | 32571:8916-8935 | MS.gene035363:intergenic | 55.0% |
| GGAGAAGCGGATGCAGAGAG+TGG | - | 32571:8895-8914 | MS.gene035363:intergenic | 60.0% | |
| ! | AAGAAGCACAGCGCAGGTGG+AGG | + | 32571:8814-8833 | MS.gene035363:CDS | 60.0% |
| ! | TCAAGAGTCCTCTGGCTCCC+TGG | + | 32571:10664-10683 | MS.gene035363:intron | 60.0% |
| ! | TGCGGGTGTTGCAGGAGAAG+CGG | - | 32571:8908-8927 | MS.gene035363:intergenic | 60.0% |
| ! | GAGGCGAAGAAGCACAGCGC+AGG | + | 32571:8808-8827 | MS.gene035363:CDS | 65.0% |
| ! | GCGAAGAAGCACAGCGCAGG+TGG | + | 32571:8811-8830 | MS.gene035363:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 32571 | gene | 8799 | 11015 | 8799 | ID=MS.gene035363 |
| 32571 | mRNA | 8799 | 11015 | 8799 | ID=MS.gene035363.t1;Parent=MS.gene035363 |
| 32571 | exon | 8799 | 9002 | 8799 | ID=MS.gene035363.t1.exon1;Parent=MS.gene035363.t1 |
| 32571 | CDS | 8799 | 9002 | 8799 | ID=cds.MS.gene035363.t1;Parent=MS.gene035363.t1 |
| 32571 | exon | 9109 | 9214 | 9109 | ID=MS.gene035363.t1.exon2;Parent=MS.gene035363.t1 |
| 32571 | CDS | 9109 | 9214 | 9109 | ID=cds.MS.gene035363.t1;Parent=MS.gene035363.t1 |
| 32571 | exon | 9722 | 9765 | 9722 | ID=MS.gene035363.t1.exon3;Parent=MS.gene035363.t1 |
| 32571 | CDS | 9722 | 9765 | 9722 | ID=cds.MS.gene035363.t1;Parent=MS.gene035363.t1 |
| 32571 | exon | 10385 | 10428 | 10385 | ID=MS.gene035363.t1.exon4;Parent=MS.gene035363.t1 |
| 32571 | CDS | 10385 | 10428 | 10385 | ID=cds.MS.gene035363.t1;Parent=MS.gene035363.t1 |
| 32571 | exon | 10913 | 11015 | 10913 | ID=MS.gene035363.t1.exon5;Parent=MS.gene035363.t1 |
| 32571 | CDS | 10913 | 11015 | 10913 | ID=cds.MS.gene035363.t1;Parent=MS.gene035363.t1 |
| Gene Sequence |
| Protein sequence |
