AlfalfaGEDB | MS.gene78481

M. sativa cultivar XinJiangDaYe / MS.gene78481

KEGG: K10418
GO: GO:0005575 GO:0005875 GO:0007017 GO:0008150 GO:0009987 GO:0030286 GO:0032991 GO:1902494
PFAM: PF01221.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene78481.t1	XP_003609697.1	98.1	103	2	0	1	103	1	103	6.30E-47	196.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene78481.t1	Q3MHR3	62.7	59	22	0	45	103	4	62	3.8e-16	85.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene78481.t1	G7JLJ9	98.1	103	2	0	1	103	1	103	4.6e-47	196.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene77160	MS.gene78481	PPI
MS.gene95803	MS.gene78481	PPI
MS.gene064351	MS.gene78481	PPI
MS.gene035825	MS.gene78481	PPI
MS.gene73093	MS.gene78481	PPI
MS.gene016178	MS.gene78481	PPI
MS.gene043643	MS.gene78481	PPI
MS.gene81099	MS.gene78481	PPI
MS.gene035826	MS.gene78481	PPI
MS.gene33030	MS.gene78481	PPI
MS.gene070829	MS.gene78481	PPI
MS.gene035828	MS.gene78481	PPI
MS.gene34372	MS.gene78481	PPI
MS.gene023152	MS.gene78481	PPI
MS.gene20245	MS.gene78481	PPI
MS.gene25633	MS.gene78481	PPI
MS.gene001031	MS.gene78481	PPI
MS.gene36490	MS.gene78481	PPI
MS.gene015202	MS.gene78481	PPI
MS.gene99872	MS.gene78481	PPI
MS.gene070831	MS.gene78481	PPI
MS.gene043595	MS.gene78481	PPI
MS.gene071366	MS.gene78481	PPI
MS.gene09543	MS.gene78481	PPI
MS.gene78481	MS.gene028302	PPI
MS.gene070946	MS.gene78481	PPI
MS.gene29908	MS.gene78481	PPI
MS.gene043593	MS.gene78481	PPI
MS.gene015206	MS.gene78481	PPI
MS.gene78481	MS.gene002746	PPI
MS.gene043818	MS.gene78481	PPI
MS.gene024860	MS.gene78481	PPI
MS.gene35155	MS.gene78481	PPI
MS.gene77163	MS.gene78481	PPI
MS.gene071364	MS.gene78481	PPI
MS.gene043645	MS.gene78481	PPI
MS.gene96225	MS.gene78481	PPI
MS.gene49473	MS.gene78481	PPI
MS.gene043821	MS.gene78481	PPI
MS.gene78481	MS.gene051574	PPI
MS.gene25632	MS.gene78481	PPI
MS.gene78481	MS.gene09540	PPI
MS.gene78481	MS.gene004435	PPI
MS.gene21834	MS.gene78481	PPI
MS.gene064353	MS.gene78481	PPI
MS.gene78481	MS.gene25631	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene78481.t1	MTR_4g120150	98.058	103	2	0	1	103	1	103	1.79e-71	209
MS.gene78481.t1	MTR_8g464690	48.333	60	29	1	46	103	5	64	4.14e-15	65.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene78481.t1	AT4G15930	58.252	103	36	3	1	103	1	96	4.74e-35	115
MS.gene78481.t1	AT4G15930	58.252	103	36	3	1	103	1	96	5.75e-35	116

Find 24 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTCTTCGTTGCGGGTGTTGC+AGG	0.303894	8.4:-11674143	None:intergenic
ATAATGACCTTCTTCGTTGC+GGG	0.323218	8.4:-11674152	None:intergenic
TGTATTGTTGGTCGCAATTT+CGG	0.332097	8.4:+11674420	MS.gene78481:CDS
CCTACTTGGCATTGTATTGT+TGG	0.395219	8.4:+11674408	MS.gene78481:CDS
ACAAGAGGCATGGTCCTACT+TGG	0.447517	8.4:+11674394	MS.gene78481:CDS
AAGAAGCACAGCGCAGGTGG+AGG	0.466246	8.4:+11674044	MS.gene78481:CDS
GCGAAGAAGCACAGCGCAGG+TGG	0.479599	8.4:+11674041	MS.gene78481:CDS
GAGGCGAAGAAGCACAGCGC+AGG	0.496399	8.4:+11674038	MS.gene78481:CDS
AGGTGGAGGAGCATCTCTAA+TGG	0.510524	8.4:+11674058	MS.gene78481:CDS
TGCAACACCCGCAACGAAGA+AGG	0.520232	8.4:+11674145	MS.gene78481:CDS
TTGCATGTCAGGGAGCATAT+CGG	0.526660	8.4:-11674183	None:intergenic
TGTTGCAGAGACTATAAAGA+AGG	0.528953	8.4:+11674365	MS.gene78481:CDS
TGCGGGTGTTGCAGGAGAAG+CGG	0.546280	8.4:-11674135	None:intergenic
TATGCTCCCTGACATGCAAA+AGG	0.547026	8.4:+11674187	MS.gene78481:CDS
GATAATGACCTTCTTCGTTG+CGG	0.566862	8.4:-11674153	None:intergenic
GGAGAAGCGGATGCAGAGAG+TGG	0.576599	8.4:-11674122	None:intergenic
CCAACAATACAATGCCAAGT+AGG	0.596782	8.4:-11674408	None:intergenic
AATTGAATTAGGCATTTGAG+AGG	0.599018	8.4:+11674325	MS.gene78481:intron
TAGGTAAAGAAAGATGAGCG+AGG	0.624160	8.4:+11674016	None:intergenic
AAGGAGTTTGACAAGAGGCA+TGG	0.631606	8.4:+11674384	MS.gene78481:CDS
GAGAGTGGAGATGTAACAAG+TGG	0.663410	8.4:-11674107	None:intergenic
AACAACGACAGCGATATCAA+CGG	0.685652	8.4:-11674213	None:intergenic
TAAAGAAGGAGTTTGACAAG+AGG	0.686443	8.4:+11674379	MS.gene78481:CDS
GTAAAGAAAGATGAGCGAGG+AGG	0.700815	8.4:+11674019	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TGAAAATTAGAAAAAATTGA+AGG	-	chr8.4:11674273-11674292	None:intergenic	15.0%
!!!	TATTGTTTTGAAATTGAATT+AGG	+	chr8.4:11674314-11674333	MS.gene78481:intron	15.0%
	AATTGAATTAGGCATTTGAG+AGG	+	chr8.4:11674325-11674344	MS.gene78481:intron	30.0%
	TAAAGAAGGAGTTTGACAAG+AGG	+	chr8.4:11674379-11674398	MS.gene78481:CDS	35.0%
	TGTTGCAGAGACTATAAAGA+AGG	+	chr8.4:11674365-11674384	MS.gene78481:CDS	35.0%
!	AACAAGTGGTTTTCGATCTT+CGG	-	chr8.4:11674096-11674115	None:intergenic	35.0%
	AACAACGACAGCGATATCAA+CGG	-	chr8.4:11674216-11674235	None:intergenic	40.0%
	ATAATGACCTTCTTCGTTGC+GGG	-	chr8.4:11674155-11674174	None:intergenic	40.0%
	CCAACAATACAATGCCAAGT+AGG	-	chr8.4:11674411-11674430	None:intergenic	40.0%
	CCTACTTGGCATTGTATTGT+TGG	+	chr8.4:11674408-11674427	MS.gene78481:CDS	40.0%
	GATAATGACCTTCTTCGTTG+CGG	-	chr8.4:11674156-11674175	None:intergenic	40.0%
	AAGGAGTTTGACAAGAGGCA+TGG	+	chr8.4:11674384-11674403	MS.gene78481:CDS	45.0%
	GAGAGTGGAGATGTAACAAG+TGG	-	chr8.4:11674110-11674129	None:intergenic	45.0%
	TATGCTCCCTGACATGCAAA+AGG	+	chr8.4:11674187-11674206	MS.gene78481:CDS	45.0%
!	TGGTTTTCGATCTTCGGTGA+GGG	-	chr8.4:11674090-11674109	None:intergenic	45.0%
!	TTGCATGTCAGGGAGCATAT+CGG	-	chr8.4:11674186-11674205	None:intergenic	45.0%
!	ACAAGAGGCATGGTCCTACT+TGG	+	chr8.4:11674394-11674413	MS.gene78481:CDS	50.0%
!	ACGGCTTCCTTTTGCATGTC+AGG	-	chr8.4:11674197-11674216	None:intergenic	50.0%
!	CGGCTTCCTTTTGCATGTCA+GGG	-	chr8.4:11674196-11674215	None:intergenic	50.0%
!	GTGGTTTTCGATCTTCGGTG+AGG	-	chr8.4:11674091-11674110	None:intergenic	50.0%
!!	AGGTGGAGGAGCATCTCTAA+TGG	+	chr8.4:11674058-11674077	MS.gene78481:CDS	50.0%
	TGCAACACCCGCAACGAAGA+AGG	+	chr8.4:11674145-11674164	MS.gene78481:CDS	55.0%
!	TTCTTCGTTGCGGGTGTTGC+AGG	-	chr8.4:11674146-11674165	None:intergenic	55.0%
	GGAGAAGCGGATGCAGAGAG+TGG	-	chr8.4:11674125-11674144	None:intergenic	60.0%
!	AAGAAGCACAGCGCAGGTGG+AGG	+	chr8.4:11674044-11674063	MS.gene78481:CDS	60.0%
!	TGCGGGTGTTGCAGGAGAAG+CGG	-	chr8.4:11674138-11674157	None:intergenic	60.0%
!	GAGGCGAAGAAGCACAGCGC+AGG	+	chr8.4:11674038-11674057	MS.gene78481:CDS	65.0%
!	GCGAAGAAGCACAGCGCAGG+TGG	+	chr8.4:11674041-11674060	MS.gene78481:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.4	gene	11674029	11674441	11674029	ID=MS.gene78481
chr8.4	mRNA	11674029	11674441	11674029	ID=MS.gene78481.t1;Parent=MS.gene78481
chr8.4	exon	11674029	11674232	11674029	ID=MS.gene78481.t1.exon1;Parent=MS.gene78481.t1
chr8.4	CDS	11674029	11674232	11674029	ID=cds.MS.gene78481.t1;Parent=MS.gene78481.t1
chr8.4	exon	11674336	11674441	11674336	ID=MS.gene78481.t1.exon2;Parent=MS.gene78481.t1
chr8.4	CDS	11674336	11674441	11674336	ID=cds.MS.gene78481.t1;Parent=MS.gene78481.t1

Gene Sequence

>MS.gene78481

ATGAGCGAGGAGGCGAAGAAGCACAGCGCAGGTGGAGGAGCATCTCTAATGGCGAAATCCCTCACCGAAGATCGAAAACCACTTGTTACATCTCCACTCTCTGCATCCGCTTCTCCTGCAACACCCGCAACGAAGAAGGTCATTATCAAGAGTGCCGATATGCTCCCTGACATGCAAAAGGAAGCCGTTGATATCGCTGTCGTTGTTAGTCTCTCTTACTCTTCAACATTCACTTCTTCTTCCTTCAATTTTTTCTAATTTTCATTTTTTTTTATTTTTTGCTAATATTGTTTTGAAATTGAATTAGGCATTTGAGAGGCACAGCGTAGAGAAAGATGTTGCAGAGACTATAAAGAAGGAGTTTGACAAGAGGCATGGTCCTACTTGGCATTGTATTGTTGGTCGCAATTTCG

Protein sequence

>MS.gene78481.t1

MSEEAKKHSAGGGASLMAKSLTEDRKPLVTSPLSASASPATPATKKVIIKSADMLPDMQKEAVDIAVVAFERHSVEKDVAETIKKEFDKRHGPTWHCIVGRNF