Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041599.t1 | XP_003609697.1 | 96.2 | 106 | 4 | 0 | 1 | 106 | 1 | 106 | 1.20E-47 | 199.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041599.t1 | Q3MHR3 | 61.3 | 62 | 24 | 0 | 45 | 106 | 4 | 65 | 7.2e-17 | 88.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041599.t1 | G7JLJ9 | 96.2 | 106 | 4 | 0 | 1 | 106 | 1 | 106 | 8.7e-48 | 199.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041599.t1 | MTR_4g120150 | 96.226 | 106 | 4 | 0 | 1 | 106 | 1 | 106 | 5.80e-72 | 213 |
| MS.gene041599.t1 | MTR_8g464690 | 49.206 | 63 | 30 | 1 | 46 | 106 | 5 | 67 | 7.25e-16 | 69.7 |
| MS.gene041599.t1 | MTR_4g107350 | 53.448 | 58 | 25 | 1 | 51 | 106 | 220 | 277 | 2.09e-11 | 61.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041599.t1 | AT4G15930 | 57.547 | 106 | 38 | 3 | 1 | 106 | 1 | 99 | 1.79e-35 | 120 |
| MS.gene041599.t1 | AT4G15930 | 58.654 | 104 | 36 | 3 | 1 | 104 | 1 | 97 | 4.82e-35 | 118 |
Find 0 sgRNAs with CRISPR-Local
Find 138 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATGTAAAAAATTAAATTCTA+AGG | + | 32569:4247-4266 | MS.gene041599:intron | 10.0% |
| !! | ATAGATTATAGATCTAATTA+AGG | - | 32569:4039-4058 | MS.gene041599:intergenic | 15.0% |
| !! | ATCAAAAGAAAATATATGTT+TGG | - | 32569:5621-5640 | MS.gene041599:intergenic | 15.0% |
| !! | GAAAGATATAAATATTTGAT+GGG | + | 32569:5689-5708 | MS.gene041599:intron | 15.0% |
| !! | TAGATTATAGATCTAATTAA+GGG | - | 32569:4038-4057 | MS.gene041599:intergenic | 15.0% |
| !! | TATAAGTTTGATATTATGAA+CGG | + | 32569:5355-5374 | MS.gene041599:intron | 15.0% |
| !! | TGAAAATTAGAAAAAATTGA+AGG | - | 32569:3853-3872 | MS.gene041599:intergenic | 15.0% |
| !! | TGAAAGATATAAATATTTGA+TGG | + | 32569:5688-5707 | MS.gene041599:intron | 15.0% |
| !!! | ATTTTTTTTTTTTTGTGATG+TGG | + | 32569:5164-5183 | MS.gene041599:intron | 15.0% |
| !!! | CTAACAACAATTTTAAAAAA+TGG | - | 32569:4309-4328 | MS.gene041599:intergenic | 15.0% |
| !!! | TATTGTTTTGAAATTGAATT+AGG | + | 32569:3894-3913 | MS.gene041599:intron | 15.0% |
| !! | AGATTATAGATCTAATTAAG+GGG | - | 32569:4037-4056 | MS.gene041599:intergenic | 20.0% |
| !! | ATTCTCATAAAGATAGAATT+GGG | - | 32569:4826-4845 | MS.gene041599:intergenic | 20.0% |
| !! | GTAGTAAATAGAAAAAATCT+AGG | - | 32569:4167-4186 | MS.gene041599:intergenic | 20.0% |
| !! | TATTCTCATAAAGATAGAAT+TGG | - | 32569:4827-4846 | MS.gene041599:intergenic | 20.0% |
| !! | TGACTTATTCATAATACAAT+CGG | - | 32569:5404-5423 | MS.gene041599:intergenic | 20.0% |
| !!! | AGTATTTAGTTTGTTAACTT+TGG | + | 32569:4632-4651 | MS.gene041599:intron | 20.0% |
| !!! | GTGTTTTATAGATTGAATTT+TGG | + | 32569:4367-4386 | MS.gene041599:intron | 20.0% |
| ! | AACATTTACACAATTTAGCA+TGG | - | 32569:4865-4884 | MS.gene041599:intergenic | 25.0% |
| ! | CAATCTATAAAACACATACA+CGG | - | 32569:4362-4381 | MS.gene041599:intergenic | 25.0% |
| ! | CATAACTATGAGTTAGAAAT+TGG | - | 32569:4134-4153 | MS.gene041599:intergenic | 25.0% |
| ! | TAACACTATTAAAGTTCTTC+AGG | - | 32569:4708-4727 | MS.gene041599:intergenic | 25.0% |
| !! | ATATCACTTCTAGAAAAGAA+GGG | + | 32569:5550-5569 | MS.gene041599:intron | 25.0% |
| !! | TATATCACTTCTAGAAAAGA+AGG | + | 32569:5549-5568 | MS.gene041599:intron | 25.0% |
| !! | TTACTACTGTTTTCTTATTG+TGG | + | 32569:4180-4199 | MS.gene041599:intron | 25.0% |
| !! | TTATGTTTTCTTCTCTCATA+TGG | + | 32569:4943-4962 | MS.gene041599:intron | 25.0% |
| !!! | AAATACTGTTTTTTCACTGA+GGG | - | 32569:4619-4638 | MS.gene041599:intergenic | 25.0% |
| !!! | AAATATTTGATGGGTTTGAT+AGG | + | 32569:5698-5717 | MS.gene041599:intron | 25.0% |
| !!! | AATATTTGATGGGTTTGATA+GGG | + | 32569:5699-5718 | MS.gene041599:intron | 25.0% |
| !!! | TAAATACTGTTTTTTCACTG+AGG | - | 32569:4620-4639 | MS.gene041599:intergenic | 25.0% |
| !!! | TTAGTTTGTTAACTTTGGAT+CGG | + | 32569:4637-4656 | MS.gene041599:intron | 25.0% |
| !!! | TTTTTTTTGTGATGTGGAAA+AGG | + | 32569:5170-5189 | MS.gene041599:intron | 25.0% |
| AAACAGGTATGTCGTTAAAT+TGG | - | 32569:4590-4609 | MS.gene041598:intergenic | 30.0% | |
| AAAGAACACAGAATACAAAG+TGG | - | 32569:4505-4524 | MS.gene041598:intergenic | 30.0% | |
| AATTGAATTAGGCATTTGAG+AGG | + | 32569:3905-3924 | MS.gene041599:intron | 30.0% | |
| AATTTAACGACATACCTGTT+TGG | + | 32569:4589-4608 | MS.gene041599:intron | 30.0% | |
| ATCTTTCATTCTACACTTCA+TGG | - | 32569:5676-5695 | MS.gene041599:intergenic | 30.0% | |
| TGTGTAGTTAACAAAATGGA+AGG | + | 32569:4203-4222 | MS.gene041599:intron | 30.0% | |
| ! | CTGTGAACAGAAAAATGTTT+TGG | - | 32569:4761-4780 | MS.gene041599:intergenic | 30.0% |
| !!! | AATACTGTTTTTTCACTGAG+GGG | - | 32569:4618-4637 | MS.gene041599:intergenic | 30.0% |
| AAGATGTTGGATGTCGAAAA+GGG | - | 32569:4797-4816 | MS.gene041598:intergenic | 35.0% | |
| AATTGGGAACAACAAGATGT+TGG | - | 32569:4810-4829 | MS.gene041599:intergenic | 35.0% | |
| AGATGAGTAATTCTTGAGTG+GGG | + | 32569:4422-4441 | MS.gene041599:intron | 35.0% | |
| CAATTGCCATAAATTCCCTA+AGG | - | 32569:5649-5668 | MS.gene041599:intergenic | 35.0% | |
| GACACACACAAAATGTATTG+AGG | - | 32569:5453-5472 | MS.gene041599:intergenic | 35.0% | |
| GAGATGAGTAATTCTTGAGT+GGG | + | 32569:4421-4440 | MS.gene041599:intron | 35.0% | |
| GTGGTGTGTAGTTAACAAAA+TGG | + | 32569:4199-4218 | MS.gene041599:intron | 35.0% | |
| GTTCCAGTTTCAAAATACCA+GGG | - | 32569:5491-5510 | MS.gene041599:intergenic | 35.0% | |
| TAAAGAAGGAGTTTGACAAG+AGG | + | 32569:3959-3978 | MS.gene041599:CDS | 35.0% | |
| TGAAGTCTAATACATCTGAG+AGG | + | 32569:5073-5092 | MS.gene041599:intron | 35.0% | |
| TGTATTCTGTGTTCTTTGCA+GGG | + | 32569:4508-4527 | MS.gene041599:intron | 35.0% | |
| TGTATTGTTGGTCGCAATTT+CGG | + | 32569:4000-4019 | MS.gene041599:CDS | 35.0% | |
| TGTTGCAGAGACTATAAAGA+AGG | + | 32569:3945-3964 | MS.gene041599:CDS | 35.0% | |
| TTGGAATGATAAACTGACAC+AGG | - | 32569:4571-4590 | MS.gene041599:intergenic | 35.0% | |
| TTGTATTCTGTGTTCTTTGC+AGG | + | 32569:4507-4526 | MS.gene041599:intron | 35.0% | |
| ! | AACAAGTGGTTTTCGATCTT+CGG | - | 32569:3676-3695 | MS.gene041599:intergenic | 35.0% |
| ! | CAGTAAATAACAAAGTGCCA+CGG | - | 32569:4918-4937 | MS.gene041599:intergenic | 35.0% |
| ! | TTCTAGAAAAGAAGGGTCAA+GGG | + | 32569:5557-5576 | MS.gene041599:intron | 35.0% |
| ! | TTCTTTTGATGTCAGCCTTA+GGG | + | 32569:5631-5650 | MS.gene041599:intron | 35.0% |
| ! | TTTCTTTTGATGTCAGCCTT+AGG | + | 32569:5630-5649 | MS.gene041599:intron | 35.0% |
| !! | ATTAAATTCTAAGGCGTGTC+TGG | + | 32569:4256-4275 | MS.gene041599:intron | 35.0% |
| !!! | CTGGTATTTTGAAACTGGAA+CGG | + | 32569:5490-5509 | MS.gene041599:intron | 35.0% |
| !!! | CTTTTCGAGTTATTTCTACC+TGG | + | 32569:4399-4418 | MS.gene041599:intron | 35.0% |
| AACAACGACAGCGATATCAA+CGG | - | 32569:3796-3815 | MS.gene041598:intergenic | 40.0% | |
| ACACAATTTAGCATGGCGTT+TGG | - | 32569:4858-4877 | MS.gene041599:intergenic | 40.0% | |
| AGTCTAATACATCTGAGAGG+AGG | + | 32569:5076-5095 | MS.gene041599:intron | 40.0% | |
| ATAATGACCTTCTTCGTTGC+GGG | - | 32569:3735-3754 | MS.gene041599:intergenic | 40.0% | |
| ATCAAAGCTTGGACTAAGCT+CGG | + | 32569:5756-5775 | MS.gene041599:CDS | 40.0% | |
| CAAGATGTTGGATGTCGAAA+AGG | - | 32569:4798-4817 | MS.gene041599:intergenic | 40.0% | |
| CCAACAATACAATGCCAAGT+AGG | - | 32569:3991-4010 | MS.gene041599:intergenic | 40.0% | |
| CCTACTTGGCATTGTATTGT+TGG | + | 32569:3988-4007 | MS.gene041599:CDS | 40.0% | |
| CGTTCCAGTTTCAAAATACC+AGG | - | 32569:5492-5511 | MS.gene041599:intergenic | 40.0% | |
| CTCAAGAATTACTCATCTCC+AGG | - | 32569:4420-4439 | MS.gene041599:intergenic | 40.0% | |
| GAAACTGGAACGGCTAATAA+CGG | + | 32569:5500-5519 | MS.gene041599:intron | 40.0% | |
| GACAGGAGTAGAAATCACAT+AGG | - | 32569:5290-5309 | MS.gene041599:intergenic | 40.0% | |
| GAGTTTGATCGTAGTGGTAT+AGG | + | 32569:4478-4497 | MS.gene041599:intron | 40.0% | |
| GATAATGACCTTCTTCGTTG+CGG | - | 32569:3736-3755 | MS.gene041599:intergenic | 40.0% | |
| GGAGATGAGTAATTCTTGAG+TGG | + | 32569:4420-4439 | MS.gene041599:intron | 40.0% | |
| GGGATAGAATAGGCTTGATA+GGG | + | 32569:4677-4696 | MS.gene041599:intron | 40.0% | |
| GTATTCTGTGTTCTTTGCAG+GGG | + | 32569:4509-4528 | MS.gene041599:intron | 40.0% | |
| GTCTAATACATCTGAGAGGA+GGG | + | 32569:5077-5096 | MS.gene041599:intron | 40.0% | |
| GTTAAGATGTGCAGAATGAC+AGG | - | 32569:5307-5326 | MS.gene041599:intergenic | 40.0% | |
| TATTCTGTGTTCTTTGCAGG+GGG | + | 32569:4510-4529 | MS.gene041599:intron | 40.0% | |
| TGGGATAGAATAGGCTTGAT+AGG | + | 32569:4676-4695 | MS.gene041599:intron | 40.0% | |
| TGTCAGCCTTAGGGAATTTA+TGG | + | 32569:5640-5659 | MS.gene041599:intron | 40.0% | |
| ! | AATTCTTGAGTGGGGTACTT+AGG | + | 32569:4430-4449 | MS.gene041599:intron | 40.0% |
| ! | AATTTGAGACTCAAGCAGTG+CGG | - | 32569:5256-5275 | MS.gene041599:intergenic | 40.0% |
| ! | ATTCTTGAGTGGGGTACTTA+GGG | + | 32569:4431-4450 | MS.gene041599:intron | 40.0% |
| ! | CTTCTAGAAAAGAAGGGTCA+AGG | + | 32569:5556-5575 | MS.gene041599:intron | 40.0% |
| ! | TTTCAAGTCGAGTTAATCCG+TGG | + | 32569:4898-4917 | MS.gene041599:intron | 40.0% |
| !! | TTTGATGGGTTTGATAGGGA+TGG | + | 32569:5703-5722 | MS.gene041599:intron | 40.0% |
| AAGGAGTTTGACAAGAGGCA+TGG | + | 32569:3964-3983 | MS.gene041599:CDS | 45.0% | |
| CCAACTGAGTTTGATCGTAG+TGG | + | 32569:4472-4491 | MS.gene041599:intron | 45.0% | |
| CCACTACGATCAAACTCAGT+TGG | - | 32569:4475-4494 | MS.gene041599:intergenic | 45.0% | |
| CTATTCCGTGGAAGAGGAAA+AGG | - | 32569:4545-4564 | MS.gene041599:intergenic | 45.0% | |
| GAGAGTGGAGATGTAACAAG+TGG | - | 32569:3690-3709 | MS.gene041599:intergenic | 45.0% | |
| GTGTGTGCTTGGGATAGAAT+AGG | + | 32569:4667-4686 | MS.gene041599:intron | 45.0% | |
| TATGCTCCCTGACATGCAAA+AGG | + | 32569:3767-3786 | MS.gene041599:CDS | 45.0% | |
| TCAAAGCTTGGACTAAGCTC+GGG | + | 32569:5757-5776 | MS.gene041599:CDS | 45.0% | |
| TTAGCATGGCGTTTGGACTA+TGG | - | 32569:4851-4870 | MS.gene041599:intergenic | 45.0% | |
| ! | AGTCCAAGCTTTGATTGTGC+TGG | - | 32569:5751-5770 | MS.gene041599:intergenic | 45.0% |
| ! | ATTTGAGACTCAAGCAGTGC+GGG | - | 32569:5255-5274 | MS.gene041599:intergenic | 45.0% |
| ! | CGGAGTTGTTTGCTGAACTT+AGG | + | 32569:5375-5394 | MS.gene041599:intron | 45.0% |
| ! | GTCCAAGCTTTGATTGTGCT+GGG | - | 32569:5750-5769 | MS.gene041599:intergenic | 45.0% |
| ! | TGGTTTTCGATCTTCGGTGA+GGG | - | 32569:3670-3689 | MS.gene041599:intergenic | 45.0% |
| ! | TTGCATGTCAGGGAGCATAT+CGG | - | 32569:3766-3785 | MS.gene041599:intergenic | 45.0% |
| !!! | AAGCTTAAGTCGCTTTTGCG+TGG | - | 32569:5329-5348 | MS.gene041599:intergenic | 45.0% |
| !!! | GCTCCCTGGTATTTTGAAAC+TGG | + | 32569:5485-5504 | MS.gene041599:intron | 45.0% |
| !! | AAAAAAAAAATATAGTATAT+TGG | - | 32569:5155-5174 | MS.gene041599:intergenic | 5.0% |
| AGCCCAGCACAATCAAAGCT+TGG | + | 32569:5745-5764 | MS.gene041599:CDS | 50.0% | |
| AGCTCGGGTTTCAGATACCA+TGG | + | 32569:5772-5791 | MS.gene041599:CDS | 50.0% | |
| CGGATTGAGTGTGTGTGCTT+GGG | + | 32569:4657-4676 | MS.gene041599:intron | 50.0% | |
| GTTCTGCTCAGCAATATCGC+AGG | - | 32569:5029-5048 | MS.gene041599:intergenic | 50.0% | |
| TAGGGATGGAGAGACTGCAA+AGG | + | 32569:5717-5736 | MS.gene041599:intron | 50.0% | |
| TCAAAATACCAGGGAGCCAG+AGG | - | 32569:5482-5501 | MS.gene041599:intergenic | 50.0% | |
| TCACTGAGGGGAAACCAAAC+AGG | - | 32569:4606-4625 | MS.gene041599:intergenic | 50.0% | |
| TCGGATTGAGTGTGTGTGCT+TGG | + | 32569:4656-4675 | MS.gene041599:intron | 50.0% | |
| TGTGTGTGTCAAGAGTCCTC+TGG | + | 32569:5463-5482 | MS.gene041599:intron | 50.0% | |
| ! | ACAAGAGGCATGGTCCTACT+TGG | + | 32569:3974-3993 | MS.gene041599:CDS | 50.0% |
| ! | ACGGCTTCCTTTTGCATGTC+AGG | - | 32569:3777-3796 | MS.gene041599:intergenic | 50.0% |
| ! | CGGCTTCCTTTTGCATGTCA+GGG | - | 32569:3776-3795 | MS.gene041599:intergenic | 50.0% |
| ! | GGGGTACTTAGGGATGACAA+TGG | + | 32569:4441-4460 | MS.gene041599:intron | 50.0% |
| ! | GGTGTCCTTTTCCTCTTCCA+CGG | + | 32569:4537-4556 | MS.gene041599:CDS | 50.0% |
| ! | GTGGTTTTCGATCTTCGGTG+AGG | - | 32569:3671-3690 | MS.gene041599:intergenic | 50.0% |
| ! | GTGTTCTTTGCAGGGGGTTT+TGG | + | 32569:4516-4535 | MS.gene041599:intron | 50.0% |
| !! | AGGTGGAGGAGCATCTCTAA+TGG | + | 32569:3638-3657 | MS.gene041599:CDS | 50.0% |
| !! | TGGCTTCTGGAACATCACCA+TGG | - | 32569:5792-5811 | MS.gene041599:intergenic | 50.0% |
| AGGCAGCTATTCCGTGGAAG+AGG | - | 32569:4551-4570 | MS.gene041599:intergenic | 55.0% | |
| TGACACAGGCAGCTATTCCG+TGG | - | 32569:4557-4576 | MS.gene041599:intergenic | 55.0% | |
| TGCAACACCCGCAACGAAGA+AGG | + | 32569:3725-3744 | MS.gene041599:CDS | 55.0% | |
| ! | GGTCAAGGGAGTGTGATCTC+TGG | + | 32569:5571-5590 | MS.gene041599:intron | 55.0% |
| ! | TTCTTCGTTGCGGGTGTTGC+AGG | - | 32569:3726-3745 | MS.gene041599:intergenic | 55.0% |
| GGAGAAGCGGATGCAGAGAG+TGG | - | 32569:3705-3724 | MS.gene041599:intergenic | 60.0% | |
| ! | AAGAAGCACAGCGCAGGTGG+AGG | + | 32569:3624-3643 | MS.gene041599:CDS | 60.0% |
| ! | TCAAGAGTCCTCTGGCTCCC+TGG | + | 32569:5471-5490 | MS.gene041599:intron | 60.0% |
| ! | TGCGGGTGTTGCAGGAGAAG+CGG | - | 32569:3718-3737 | MS.gene041599:intergenic | 60.0% |
| ! | GAGGCGAAGAAGCACAGCGC+AGG | + | 32569:3618-3637 | MS.gene041599:CDS | 65.0% |
| ! | GCGAAGAAGCACAGCGCAGG+TGG | + | 32569:3621-3640 | MS.gene041599:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 32569 | gene | 3609 | 5822 | 3609 | ID=MS.gene041599 |
| 32569 | mRNA | 3609 | 5822 | 3609 | ID=MS.gene041599.t1;Parent=MS.gene041599 |
| 32569 | exon | 3609 | 3812 | 3609 | ID=MS.gene041599.t1.exon1;Parent=MS.gene041599.t1 |
| 32569 | CDS | 3609 | 3812 | 3609 | ID=cds.MS.gene041599.t1;Parent=MS.gene041599.t1 |
| 32569 | exon | 3916 | 4021 | 3916 | ID=MS.gene041599.t1.exon2;Parent=MS.gene041599.t1 |
| 32569 | CDS | 3916 | 4021 | 3916 | ID=cds.MS.gene041599.t1;Parent=MS.gene041599.t1 |
| 32569 | exon | 4529 | 4572 | 4529 | ID=MS.gene041599.t1.exon3;Parent=MS.gene041599.t1 |
| 32569 | CDS | 4529 | 4572 | 4529 | ID=cds.MS.gene041599.t1;Parent=MS.gene041599.t1 |
| 32569 | exon | 5192 | 5235 | 5192 | ID=MS.gene041599.t1.exon4;Parent=MS.gene041599.t1 |
| 32569 | CDS | 5192 | 5235 | 5192 | ID=cds.MS.gene041599.t1;Parent=MS.gene041599.t1 |
| 32569 | exon | 5720 | 5822 | 5720 | ID=MS.gene041599.t1.exon5;Parent=MS.gene041599.t1 |
| 32569 | CDS | 5720 | 5822 | 5720 | ID=cds.MS.gene041599.t1;Parent=MS.gene041599.t1 |
| Gene Sequence |
| Protein sequence |
