AlfalfaGEDB | MS.gene035362

M. sativa cultivar XinJiangDaYe / MS.gene035362

KEGG: K10418
GO: GO:0005575 GO:0005875 GO:0007017 GO:0008150 GO:0009987 GO:0030286 GO:0032991 GO:1902494
PFAM: PF01221.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene035362.t1	XP_003609697.1	98.1	103	2	0	1	103	1	103	6.30E-47	196.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene035362.t1	Q3MHR3	62.7	59	22	0	45	103	4	62	3.8e-16	85.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene035362.t1	G7JLJ9	98.1	103	2	0	1	103	1	103	4.6e-47	196.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene035362	MS.gene043643	PPI
MS.gene035362	MS.gene49473	PPI
MS.gene035362	MS.gene064353	PPI
MS.gene071364	MS.gene035362	PPI
MS.gene96225	MS.gene035362	PPI
MS.gene035362	MS.gene09543	PPI
MS.gene21834	MS.gene035362	PPI
MS.gene035362	MS.gene29908	PPI
MS.gene25632	MS.gene035362	PPI
MS.gene035362	MS.gene36490	PPI
MS.gene035826	MS.gene035362	PPI
MS.gene035362	MS.gene77160	PPI
MS.gene035362	MS.gene070831	PPI
MS.gene035362	MS.gene043595	PPI
MS.gene035362	MS.gene001031	PPI
MS.gene035362	MS.gene35155	PPI
MS.gene035362	MS.gene81099	PPI
MS.gene99872	MS.gene035362	PPI
MS.gene035362	MS.gene064351	PPI
MS.gene035362	MS.gene028302	PPI
MS.gene071366	MS.gene035362	PPI
MS.gene035362	MS.gene043593	PPI
MS.gene035362	MS.gene002746	PPI
MS.gene33030	MS.gene035362	PPI
MS.gene035362	MS.gene043821	PPI
MS.gene035828	MS.gene035362	PPI
MS.gene035362	MS.gene20245	PPI
MS.gene023152	MS.gene035362	PPI
MS.gene035362	MS.gene043645	PPI
MS.gene035362	MS.gene070829	PPI
MS.gene035362	MS.gene95803	PPI
MS.gene035362	MS.gene016178	PPI
MS.gene035362	MS.gene035825	PPI
MS.gene035362	MS.gene77163	PPI
MS.gene035362	MS.gene34372	PPI
MS.gene035362	MS.gene043818	PPI
MS.gene035362	MS.gene051574	PPI
MS.gene035362	MS.gene09540	PPI
MS.gene070946	MS.gene035362	PPI
MS.gene035362	MS.gene015202	PPI
MS.gene035362	MS.gene004435	PPI
MS.gene035362	MS.gene73093	PPI
MS.gene015206	MS.gene035362	PPI
MS.gene035362	MS.gene25633	PPI
MS.gene035362	MS.gene25631	PPI
MS.gene035362	MS.gene024860	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene035362.t1	MTR_4g120150	98.058	103	2	0	1	103	1	103	1.79e-71	209
MS.gene035362.t1	MTR_8g464690	48.333	60	29	1	46	103	5	64	4.14e-15	65.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene035362.t1	AT4G15930	58.252	103	36	3	1	103	1	96	4.74e-35	115
MS.gene035362.t1	AT4G15930	58.252	103	36	3	1	103	1	96	5.75e-35	116

Find 24 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTCTTCGTTGCGGGTGTTGC+AGG	0.303894	8.4:+11613175	None:intergenic
ATAATGACCTTCTTCGTTGC+GGG	0.323218	8.4:+11613166	None:intergenic
TGTATTGTTGGTCGCAATTT+CGG	0.332097	8.4:-11612895	None:intergenic
CCTACTTGGCATTGTATTGT+TGG	0.395219	8.4:-11612907	MS.gene035362:CDS
ACAAGAGGCATGGTCCTACT+TGG	0.447517	8.4:-11612921	MS.gene035362:CDS
AAGAAGCACAGCGCAGGTGG+AGG	0.466246	8.4:-11613274	MS.gene035362:CDS
GCGAAGAAGCACAGCGCAGG+TGG	0.479599	8.4:-11613277	MS.gene035362:CDS
GAGGCGAAGAAGCACAGCGC+AGG	0.496399	8.4:-11613280	MS.gene035362:CDS
AGGTGGAGGAGCATCTCTAA+TGG	0.510524	8.4:-11613260	MS.gene035362:CDS
TGCAACACCCGCAACGAAGA+AGG	0.520232	8.4:-11613173	MS.gene035362:CDS
TTGCATGTCAGGGAGCATAT+CGG	0.526660	8.4:+11613135	None:intergenic
TGTTGCAGAGACTATAAAGA+AGG	0.528953	8.4:-11612950	MS.gene035362:CDS
TGCGGGTGTTGCAGGAGAAG+CGG	0.546280	8.4:+11613183	None:intergenic
TATGCTCCCTGACATGCAAA+AGG	0.547026	8.4:-11613131	MS.gene035362:CDS
GATAATGACCTTCTTCGTTG+CGG	0.566862	8.4:+11613165	None:intergenic
GGAGAAGCGGATGCAGAGAG+TGG	0.576599	8.4:+11613196	None:intergenic
CCAACAATACAATGCCAAGT+AGG	0.596782	8.4:+11612907	None:intergenic
AATTGAATTAGGCATTTGAG+AGG	0.599018	8.4:-11612990	MS.gene035362:intron
TAGGTAAAGAAAGATGAGCG+AGG	0.624160	8.4:-11613302	None:intergenic
AAGGAGTTTGACAAGAGGCA+TGG	0.631606	8.4:-11612931	MS.gene035362:CDS
GAGAGTGGAGATGTAACAAG+TGG	0.663410	8.4:+11613211	None:intergenic
AACAACGACAGCGATATCAA+CGG	0.685652	8.4:+11613105	None:intergenic
TAAAGAAGGAGTTTGACAAG+AGG	0.686443	8.4:-11612936	MS.gene035362:CDS
GTAAAGAAAGATGAGCGAGG+AGG	0.700815	8.4:-11613299	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAATTAGAAAAAATTGAAGA+AGG	+	chr8.4:11613140-11613159	None:intergenic	15.0%
!!!	TATTGTTTTGAAATTGAATT+AGG	-	chr8.4:11613184-11613203	MS.gene035362:CDS	15.0%
	AATTGAATTAGGCATTTGAG+AGG	-	chr8.4:11613195-11613214	MS.gene035362:CDS	30.0%
	TAAAGAAGGAGTTTGACAAG+AGG	-	chr8.4:11613249-11613268	MS.gene035362:CDS	35.0%
	TGTTGCAGAGACTATAAAGA+AGG	-	chr8.4:11613235-11613254	MS.gene035362:CDS	35.0%
!	AACAAGTGGTTTTCGATCTT+CGG	+	chr8.4:11612963-11612982	None:intergenic	35.0%
	AACAACGACAGCGATATCAA+CGG	+	chr8.4:11613083-11613102	None:intergenic	40.0%
	ATAATGACCTTCTTCGTTGC+GGG	+	chr8.4:11613022-11613041	None:intergenic	40.0%
	CCAACAATACAATGCCAAGT+AGG	+	chr8.4:11613281-11613300	None:intergenic	40.0%
	CCTACTTGGCATTGTATTGT+TGG	-	chr8.4:11613278-11613297	MS.gene035362:CDS	40.0%
	GATAATGACCTTCTTCGTTG+CGG	+	chr8.4:11613023-11613042	None:intergenic	40.0%
	AAGGAGTTTGACAAGAGGCA+TGG	-	chr8.4:11613254-11613273	MS.gene035362:CDS	45.0%
	GAGAGTGGAGATGTAACAAG+TGG	+	chr8.4:11612977-11612996	None:intergenic	45.0%
	TATGCTCCCTGACATGCAAA+AGG	-	chr8.4:11613054-11613073	MS.gene035362:intron	45.0%
!	TGGTTTTCGATCTTCGGTGA+GGG	+	chr8.4:11612957-11612976	None:intergenic	45.0%
!	TTGCATGTCAGGGAGCATAT+CGG	+	chr8.4:11613053-11613072	None:intergenic	45.0%
!	ACAAGAGGCATGGTCCTACT+TGG	-	chr8.4:11613264-11613283	MS.gene035362:CDS	50.0%
!	ACGGCTTCCTTTTGCATGTC+AGG	+	chr8.4:11613064-11613083	None:intergenic	50.0%
!	CGGCTTCCTTTTGCATGTCA+GGG	+	chr8.4:11613063-11613082	None:intergenic	50.0%
!	GTGGTTTTCGATCTTCGGTG+AGG	+	chr8.4:11612958-11612977	None:intergenic	50.0%
!!	AGGTGGAGGAGCATCTCTAA+TGG	-	chr8.4:11612925-11612944	MS.gene035362:CDS	50.0%
	TGCAACACCCGCAACGAAGA+AGG	-	chr8.4:11613012-11613031	MS.gene035362:intron	55.0%
!	TTCTTCGTTGCGGGTGTTGC+AGG	+	chr8.4:11613013-11613032	None:intergenic	55.0%
	GGAGAAGCGGATGCAGAGAG+TGG	+	chr8.4:11612992-11613011	None:intergenic	60.0%
!	AAGAAGCACAGCGCAGGTGG+AGG	-	chr8.4:11612911-11612930	MS.gene035362:CDS	60.0%
!	TGCGGGTGTTGCAGGAGAAG+CGG	+	chr8.4:11613005-11613024	None:intergenic	60.0%
!	GAGGCGAAGAAGCACAGCGC+AGG	-	chr8.4:11612905-11612924	MS.gene035362:CDS	65.0%
!	GCGAAGAAGCACAGCGCAGG+TGG	-	chr8.4:11612908-11612927	MS.gene035362:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.4	gene	11612896	11613311	11612896	ID=MS.gene035362
chr8.4	mRNA	11612896	11613311	11612896	ID=MS.gene035362.t1;Parent=MS.gene035362
chr8.4	exon	11613108	11613311	11613108	ID=MS.gene035362.t1.exon1;Parent=MS.gene035362.t1
chr8.4	CDS	11613108	11613311	11613108	ID=cds.MS.gene035362.t1;Parent=MS.gene035362.t1
chr8.4	exon	11612896	11613001	11612896	ID=MS.gene035362.t1.exon2;Parent=MS.gene035362.t1
chr8.4	CDS	11612896	11613001	11612896	ID=cds.MS.gene035362.t1;Parent=MS.gene035362.t1

Gene Sequence

>MS.gene035362

ATGAGCGAGGAGGCGAAGAAGCACAGCGCAGGTGGAGGAGCATCTCTAATGGCGAAATCCCTCACCGAAGATCGAAAACCACTTGTTACATCTCCACTCTCTGCATCCGCTTCTCCTGCAACACCCGCAACGAAGAAGGTCATTATCAAGAGTGCCGATATGCTCCCTGACATGCAAAAGGAAGCCGTTGATATCGCTGTCGTTGTTAGTCTCTCTTACTCTTCAACATTCACTTCTTCTTCCTTCTTCAATTTTTTCTAATTTTCATTTTTTTTTATTTTTTGCTAATATTGTTTTGAAATTGAATTAGGCATTTGAGAGGCACAGCGTAGAGAAAGATGTTGCAGAGACTATAAAGAAGGAGTTTGACAAGAGGCATGGTCCTACTTGGCATTGTATTGTTGGTCGCAATTTCG

Protein sequence

>MS.gene035362.t1

MSEEAKKHSAGGGASLMAKSLTEDRKPLVTSPLSASASPATPATKKVIIKSADMLPDMQKEAVDIAVVAFERHSVEKDVAETIKKEFDKRHGPTWHCIVGRNF