Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene018905.t1 | RHN66515.1 | 96.4 | 195 | 7 | 0 | 1 | 195 | 19 | 213 | 2.20E-101 | 378.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene018905.t1 | O13737 | 35.0 | 180 | 102 | 3 | 6 | 184 | 4 | 169 | 7.7e-18 | 92.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene018905.t1 | A0A072UTY5 | 96.4 | 195 | 7 | 0 | 1 | 195 | 1 | 195 | 1.6e-101 | 378.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene067529 | MS.gene018905 | PPI |
| MS.gene067528 | MS.gene018905 | PPI |
| MS.gene018908 | MS.gene018905 | PPI |
| MS.gene067530 | MS.gene018905 | PPI |
| MS.gene03534 | MS.gene018905 | PPI |
| MS.gene018905 | MS.gene018908 | PPI |
| MS.gene03531 | MS.gene018905 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene018905.t1 | MTR_3g435200 | 96.410 | 195 | 7 | 0 | 1 | 195 | 1 | 195 | 1.82e-138 | 384 |
| MS.gene018905.t1 | MTR_3g435170 | 88.649 | 185 | 19 | 1 | 1 | 183 | 1 | 185 | 8.40e-116 | 327 |
| MS.gene018905.t1 | MTR_3g435230 | 75.127 | 197 | 47 | 1 | 1 | 195 | 1 | 197 | 7.59e-109 | 310 |
| MS.gene018905.t1 | MTR_3g435250 | 69.543 | 197 | 58 | 1 | 1 | 195 | 1 | 197 | 1.31e-99 | 286 |
| MS.gene018905.t1 | MTR_5g041930 | 63.636 | 198 | 68 | 1 | 2 | 195 | 5 | 202 | 3.77e-90 | 263 |
| MS.gene018905.t1 | MTR_3g435230 | 71.852 | 135 | 36 | 1 | 1 | 133 | 1 | 135 | 3.44e-65 | 197 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene018905.t1 | AT1G18720 | 61.616 | 198 | 71 | 1 | 1 | 193 | 5 | 202 | 4.59e-86 | 253 |
| MS.gene018905.t1 | AT1G18720 | 61.616 | 198 | 71 | 1 | 1 | 193 | 5 | 202 | 4.59e-86 | 253 |
| MS.gene018905.t1 | AT1G74440 | 58.031 | 193 | 76 | 1 | 1 | 188 | 5 | 197 | 4.70e-80 | 237 |
| MS.gene018905.t1 | AT1G74440 | 58.031 | 193 | 76 | 1 | 1 | 188 | 5 | 197 | 4.70e-80 | 237 |
Find 0 sgRNAs with CRISPR-Local
Find 107 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTATAAAGAATAAAGATA+CGG | + | 23448:10226-10245 | None:intergenic | 10.0% |
| !! | TTATTCTTTATAATTATACA+GGG | - | 23448:10231-10250 | MS.gene018905:CDS | 10.0% |
| !! | TTTATTCTTTATAATTATAC+AGG | - | 23448:10230-10249 | MS.gene018905:CDS | 10.0% |
| !! | AAAGAAATTAATGCTTAATT+GGG | + | 23448:9371-9390 | None:intergenic | 15.0% |
| !! | AAGTTGAAATTATAATACAA+AGG | - | 23448:10113-10132 | MS.gene018905:CDS | 15.0% |
| !! | ACATAACCATATAATAAAAA+TGG | - | 23448:9445-9464 | MS.gene018905:intron | 15.0% |
| !! | TAAAGAAATTAATGCTTAAT+TGG | + | 23448:9372-9391 | None:intergenic | 15.0% |
| !! | TTGTATTATAATTTCAACTT+AGG | + | 23448:10113-10132 | None:intergenic | 15.0% |
| !!! | ATGTTTTTCTAAAATAAATC+AGG | + | 23448:10022-10041 | None:intergenic | 15.0% |
| !!! | TTTTATATTATAAACCCTAA+GGG | - | 23448:9859-9878 | MS.gene018905:CDS | 15.0% |
| !!! | ATGTGAATTATGATGTTTTT+TGG | + | 23448:9062-9081 | None:intergenic | 20.0% |
| !!! | ATTAAGTCACAACATATTTT+TGG | + | 23448:9506-9525 | None:intergenic | 20.0% |
| !!! | GTTTTATATTATAAACCCTA+AGG | - | 23448:9858-9877 | MS.gene018905:CDS | 20.0% |
| !!! | TGTGAATTATGATGTTTTTT+GGG | + | 23448:9061-9080 | None:intergenic | 20.0% |
| !!! | TTTCTTTACATTTTATTTCG+AGG | - | 23448:9408-9427 | MS.gene018905:intron | 20.0% |
| ! | AATTTACCTCAAACACATAA+TGG | + | 23448:9599-9618 | None:intergenic | 25.0% |
| ! | ACATAACAATGAAAAAATGG+TGG | + | 23448:9137-9156 | None:intergenic | 25.0% |
| ! | AGGACATAACAATGAAAAAA+TGG | + | 23448:9140-9159 | None:intergenic | 25.0% |
| ! | CATATAATAAAAATGGCATG+AGG | - | 23448:9452-9471 | MS.gene018905:intron | 25.0% |
| ! | TAGATGCTAATATCAAAGAA+TGG | - | 23448:10387-10406 | MS.gene018905:CDS | 25.0% |
| !!! | CTCATGCCATTTTTATTATA+TGG | + | 23448:9454-9473 | None:intergenic | 25.0% |
| AAAAATGAACGCACTATGTT+TGG | + | 23448:10193-10212 | None:intergenic | 30.0% | |
| ATATCCTGGATTTCAGAAAA+AGG | - | 23448:10352-10371 | MS.gene018905:CDS | 30.0% | |
| ATTTATGACTATGATCTTGC+AGG | - | 23448:9527-9546 | MS.gene018905:intron | 30.0% | |
| CTAGGTTAAAAATCAAGACA+GGG | + | 23448:9175-9194 | None:intergenic | 30.0% | |
| GTTTCTTTCACAATGAAACT+AGG | - | 23448:10275-10294 | MS.gene018905:CDS | 30.0% | |
| TAATTCACATCCTCTTTGTT+TGG | - | 23448:9072-9091 | MS.gene018905:CDS | 30.0% | |
| TCCAGAGTAATGTTGTAATA+TGG | - | 23448:9815-9834 | MS.gene018905:intron | 30.0% | |
| TCCATATTACAACATTACTC+TGG | + | 23448:9819-9838 | None:intergenic | 30.0% | |
| ! | ATTTCAACTTAGGTTGTGTT+TGG | + | 23448:10103-10122 | None:intergenic | 30.0% |
| ! | GTTCATATCCTATTGATGAT+TGG | + | 23448:10331-10350 | None:intergenic | 30.0% |
| !!! | AGTTTTGTTGCAAATTCCAT+TGG | - | 23448:9296-9315 | MS.gene018905:intron | 30.0% |
| !!! | TTTTTCTGAAATCCAGGATA+TGG | + | 23448:10353-10372 | None:intergenic | 30.0% |
| AGACAGGGTGGATAAAATTA+AGG | + | 23448:9160-9179 | None:intergenic | 35.0% | |
| ATAGGATATGAACCATATCC+TGG | - | 23448:10338-10357 | MS.gene018905:CDS | 35.0% | |
| CCTAGGTTAAAAATCAAGAC+AGG | + | 23448:9176-9195 | None:intergenic | 35.0% | |
| GGTTCTTACCTTTCTTTGTT+GGG | - | 23448:9265-9284 | MS.gene018905:intron | 35.0% | |
| TAGATCCTCCAATCATCAAT+AGG | - | 23448:10320-10339 | MS.gene018905:CDS | 35.0% | |
| TATGATCTTGCAGGTTGTAT+TGG | - | 23448:9536-9555 | MS.gene018905:intron | 35.0% | |
| TGTGTTTGGATTGAGGATTT+GGG | + | 23448:10089-10108 | None:intergenic | 35.0% | |
| TGTTTGTTTAGTATGACTCG+TGG | - | 23448:9773-9792 | MS.gene018905:intron | 35.0% | |
| TTGTGTTTGGATTGAGGATT+TGG | + | 23448:10090-10109 | None:intergenic | 35.0% | |
| ! | AAAATGGTGGAGTGAAGTAA+AGG | + | 23448:9124-9143 | None:intergenic | 35.0% |
| ! | CATATCCTATTGATGATTGG+AGG | + | 23448:10328-10347 | None:intergenic | 35.0% |
| ! | CATTGCTCAAGCTTTTCTAA+TGG | - | 23448:9718-9737 | MS.gene018905:CDS | 35.0% |
| ! | CCTGTCTTGATTTTTAACCT+AGG | - | 23448:9173-9192 | MS.gene018905:intron | 35.0% |
| ! | TTTTGGCCATTATGTGTTTG+AGG | - | 23448:9590-9609 | MS.gene018905:intron | 35.0% |
| !! | TATAAAAGCTGGTTCGTTTG+CGG | - | 23448:9241-9260 | MS.gene018905:intron | 35.0% |
| !!! | GATGTCTCACATTTTTTTCG+CGG | - | 23448:9345-9364 | MS.gene018905:intron | 35.0% |
| ACAAGAGCAGTGAAAAGGAT+TGG | + | 23448:9098-9117 | None:intergenic | 40.0% | |
| ACCTCAAGAACCACGAAAAA+AGG | + | 23448:9746-9765 | None:intergenic | 40.0% | |
| ACTCGTGGTTACTGATAAGT+GGG | - | 23448:9788-9807 | MS.gene018905:intron | 40.0% | |
| AGAACCAACCCAACAAAGAA+AGG | + | 23448:9276-9295 | None:intergenic | 40.0% | |
| CGGTTCTTACCTTTCTTTGT+TGG | - | 23448:9264-9283 | MS.gene018905:intron | 40.0% | |
| CTTACCTTTCTTTGTTGGGT+TGG | - | 23448:9269-9288 | MS.gene018905:intron | 40.0% | |
| GGTAAACAAGAGCAGTGAAA+AGG | + | 23448:9103-9122 | None:intergenic | 40.0% | |
| GGTTAAAAATCAAGACAGGG+TGG | + | 23448:9172-9191 | None:intergenic | 40.0% | |
| TTCGTATTCGCTGTTGTCTA+CGG | - | 23448:9197-9216 | MS.gene018905:intron | 40.0% | |
| TTTGGATTGAGGATTTGGGA+GGG | + | 23448:10085-10104 | None:intergenic | 40.0% | |
| ! | ATAAACCCTAAGGGTGTGTT+TGG | - | 23448:9868-9887 | MS.gene018905:CDS | 40.0% |
| ! | CGTATCATGCTTTTCAGTGA+TGG | + | 23448:9628-9647 | None:intergenic | 40.0% |
| ! | CTTAGGTTGTGTTTGGATTG+AGG | + | 23448:10096-10115 | None:intergenic | 40.0% |
| ! | GCTGCTTTGGATATAAAAGC+TGG | - | 23448:9230-9249 | MS.gene018905:intron | 40.0% |
| ! | TGGATTTCAGAAAAAGGTGC+AGG | - | 23448:10358-10377 | MS.gene018905:CDS | 40.0% |
| !! | AAAGGATTGGCCAAACAAAG+AGG | + | 23448:9085-9104 | None:intergenic | 40.0% |
| !! | GAAAAGCACTTTGCCTTCTA+CGG | - | 23448:9020-9039 | MS.gene018905:CDS | 40.0% |
| !! | GGCTACTCAGTTGTTTTGTT+GGG | - | 23448:9557-9576 | MS.gene018905:intron | 40.0% |
| !! | TGGCTACTCAGTTGTTTTGT+TGG | - | 23448:9556-9575 | MS.gene018905:intron | 40.0% |
| !!! | TCCTTTTTTCGTGGTTCTTG+AGG | - | 23448:9742-9761 | MS.gene018905:intron | 40.0% |
| !!! | TCTAATGGCTCCTTTTTTCG+TGG | - | 23448:9733-9752 | MS.gene018905:intron | 40.0% |
| !!! | TGCACCTTTTTCTGAAATCC+AGG | + | 23448:10359-10378 | None:intergenic | 40.0% |
| CAACAGCGAATACGAAACCT+AGG | + | 23448:9193-9212 | None:intergenic | 45.0% | |
| CGCTTTGCAGTTGAATACGT+TGG | - | 23448:10145-10164 | MS.gene018905:CDS | 45.0% | |
| GAAACCAGTCGAGATCAGAT+AGG | + | 23448:10260-10279 | None:intergenic | 45.0% | |
| GACTCGTGGTTACTGATAAG+TGG | - | 23448:9787-9806 | MS.gene018905:intron | 45.0% | |
| GCAATGTTGTCCAATAGAGC+TGG | + | 23448:9704-9723 | None:intergenic | 45.0% | |
| GCTGTGATAAGAACCGTAGA+AGG | + | 23448:9036-9055 | None:intergenic | 45.0% | |
| GTTTGGATTGAGGATTTGGG+AGG | + | 23448:10086-10105 | None:intergenic | 45.0% | |
| TCCATCCAAACACACCCTTA+GGG | + | 23448:9876-9895 | None:intergenic | 45.0% | |
| TGTGTTTGGATGGAGGGTTT+AGG | - | 23448:9882-9901 | MS.gene018905:intron | 45.0% | |
| TTGGATTGAGGATTTGGGAG+GGG | + | 23448:10084-10103 | None:intergenic | 45.0% | |
| TTTGGATGGAGGGTTTAGGA+GGG | - | 23448:9886-9905 | MS.gene018905:intron | 45.0% | |
| ! | TAAGGGTGTGTTTGGATGGA+GGG | - | 23448:9876-9895 | MS.gene018905:intron | 45.0% |
| ! | TGTTGGGCAGGACAGATTTT+TGG | - | 23448:9573-9592 | MS.gene018905:intron | 45.0% |
| ! | TTCAGAAAAAGGTGCAGGCA+AGG | - | 23448:10363-10382 | MS.gene018905:CDS | 45.0% |
| !! | ACTCAGTTGTTTTGTTGGGC+AGG | - | 23448:9561-9580 | MS.gene018905:intron | 45.0% |
| !!! | TAATTTTCTTTTTTAAATTA+CGG | + | 23448:10055-10074 | None:intergenic | 5.0% |
| !!! | TATTTTTCAATTTTAAATTA+CGG | - | 23448:9926-9945 | MS.gene018905:intron | 5.0% |
| ATTGAGGATTTGGGAGGGGA+GGG | + | 23448:10080-10099 | None:intergenic | 50.0% | |
| CGTGGTTACTGATAAGTGGG+TGG | - | 23448:9791-9810 | MS.gene018905:intron | 50.0% | |
| CTCCATCCAAACACACCCTT+AGG | + | 23448:9877-9896 | None:intergenic | 50.0% | |
| GAAACGTGCACCAGCTCTAT+TGG | - | 23448:9691-9710 | MS.gene018905:CDS | 50.0% | |
| GTTGCCTATCTGATCTCGAC+TGG | - | 23448:10253-10272 | MS.gene018905:CDS | 50.0% | |
| GTTTGGATGGAGGGTTTAGG+AGG | - | 23448:9885-9904 | MS.gene018905:intron | 50.0% | |
| TTGGATGGAGGGTTTAGGAG+GGG | - | 23448:9887-9906 | MS.gene018905:intron | 50.0% | |
| ! | ACCCTAAGGGTGTGTTTGGA+TGG | - | 23448:9872-9891 | MS.gene018905:CDS | 50.0% |
| ! | CTAAGGGTGTGTTTGGATGG+AGG | - | 23448:9875-9894 | MS.gene018905:intron | 50.0% |
| !! | AAAAGCTGGTTCGTTTGCGG+CGG | - | 23448:9244-9263 | MS.gene018905:intron | 50.0% |
| AGGGTTTAGGAGGGGAAGAG+AGG | - | 23448:9895-9914 | MS.gene018905:intron | 55.0% | |
| CCAAGCGAGTTCGTAGCCAA+TGG | + | 23448:9315-9334 | None:intergenic | 55.0% | |
| CGGTCTCTTCTACGCTGCTT+TGG | - | 23448:9217-9236 | MS.gene018905:intron | 55.0% | |
| GATTGAGGATTTGGGAGGGG+AGG | + | 23448:10081-10100 | None:intergenic | 55.0% | |
| GGGTTTAGGAGGGGAAGAGA+GGG | - | 23448:9896-9915 | MS.gene018905:intron | 55.0% | |
| GGTTTAGGAGGGGAAGAGAG+GGG | - | 23448:9897-9916 | MS.gene018905:intron | 55.0% | |
| TGGCTACGAACTCGCTTGGA+AGG | - | 23448:9316-9335 | MS.gene018905:intron | 55.0% | |
| ! | CCATTGGCTACGAACTCGCT+TGG | - | 23448:9312-9331 | MS.gene018905:intron | 55.0% |
| TAGGAGGGGAAGAGAGGGGA+CGG | - | 23448:9901-9920 | MS.gene018905:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 23448 | gene | 9002 | 10439 | 9002 | ID=MS.gene018905 |
| 23448 | mRNA | 9002 | 10439 | 9002 | ID=MS.gene018905.t1;Parent=MS.gene018905 |
| 23448 | exon | 10104 | 10439 | 10104 | ID=MS.gene018905.t1.exon1;Parent=MS.gene018905.t1 |
| 23448 | CDS | 10104 | 10439 | 10104 | ID=cds.MS.gene018905.t1;Parent=MS.gene018905.t1 |
| 23448 | exon | 9830 | 9892 | 9830 | ID=MS.gene018905.t1.exon2;Parent=MS.gene018905.t1 |
| 23448 | CDS | 9830 | 9892 | 9830 | ID=cds.MS.gene018905.t1;Parent=MS.gene018905.t1 |
| 23448 | exon | 9678 | 9749 | 9678 | ID=MS.gene018905.t1.exon3;Parent=MS.gene018905.t1 |
| 23448 | CDS | 9678 | 9749 | 9678 | ID=cds.MS.gene018905.t1;Parent=MS.gene018905.t1 |
| 23448 | exon | 9002 | 9118 | 9002 | ID=MS.gene018905.t1.exon4;Parent=MS.gene018905.t1 |
| 23448 | CDS | 9002 | 9118 | 9002 | ID=cds.MS.gene018905.t1;Parent=MS.gene018905.t1 |
| Gene Sequence |
| Protein sequence |
