Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067529.t1 | RHN66515.1 | 95.9 | 195 | 8 | 0 | 1 | 195 | 19 | 213 | 3.80E-101 | 377.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067529.t1 | O13737 | 34.4 | 180 | 103 | 3 | 6 | 184 | 4 | 169 | 1.3e-17 | 91.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067529.t1 | A0A072UTY5 | 95.9 | 195 | 8 | 0 | 1 | 195 | 1 | 195 | 2.7e-101 | 377.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene03534 | MS.gene067529 | PPI |
| MS.gene067529 | MS.gene03534 | PPI |
| MS.gene067529 | MS.gene018905 | PPI |
| MS.gene067529 | MS.gene03531 | PPI |
| MS.gene067529 | MS.gene018908 | PPI |
| MS.gene067529 | MS.gene067528 | PPI |
| MS.gene067530 | MS.gene067529 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067529.t1 | MTR_3g435200 | 95.897 | 195 | 8 | 0 | 1 | 195 | 1 | 195 | 6.44e-138 | 383 |
| MS.gene067529.t1 | MTR_3g435170 | 88.108 | 185 | 20 | 1 | 1 | 183 | 1 | 185 | 2.51e-115 | 326 |
| MS.gene067529.t1 | MTR_3g435230 | 74.619 | 197 | 48 | 1 | 1 | 195 | 1 | 197 | 2.56e-108 | 308 |
| MS.gene067529.t1 | MTR_3g435250 | 69.543 | 197 | 58 | 1 | 1 | 195 | 1 | 197 | 2.50e-99 | 286 |
| MS.gene067529.t1 | MTR_5g041930 | 63.636 | 198 | 68 | 1 | 2 | 195 | 5 | 202 | 2.11e-90 | 263 |
| MS.gene067529.t1 | MTR_3g435230 | 71.111 | 135 | 37 | 1 | 1 | 133 | 1 | 135 | 1.18e-64 | 196 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067529.t1 | AT1G18720 | 61.616 | 198 | 71 | 1 | 1 | 193 | 5 | 202 | 2.81e-86 | 253 |
| MS.gene067529.t1 | AT1G18720 | 61.616 | 198 | 71 | 1 | 1 | 193 | 5 | 202 | 2.81e-86 | 253 |
| MS.gene067529.t1 | AT1G74440 | 58.031 | 193 | 76 | 1 | 1 | 188 | 5 | 197 | 2.72e-80 | 238 |
| MS.gene067529.t1 | AT1G74440 | 58.031 | 193 | 76 | 1 | 1 | 188 | 5 | 197 | 2.72e-80 | 238 |
Find 35 sgRNAs with CRISPR-Local
Find 87 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAACGTGCACCAGCTTTAT+TGG | 0.194865 | 3.2:-26511951 | MS.gene067529:CDS |
| AGACAGGGTGGATAAAATTA+AGG | 0.262797 | 3.2:+26512484 | None:intergenic |
| CGGTTCTTACCTTTCTTTGT+TGG | 0.339825 | 3.2:-26512377 | MS.gene067529:CDS |
| TAATTCACATCCTCTTTGTT+TGG | 0.345262 | 3.2:-26512569 | MS.gene067529:CDS |
| ATATCCTGGATTTCAGAAAA+AGG | 0.356957 | 3.2:-26511528 | MS.gene067529:CDS |
| GGTTCTTACCTTTCTTTGTT+GGG | 0.381903 | 3.2:-26512376 | MS.gene067529:CDS |
| CGGCCTCTTCTACGCTGCTT+TGG | 0.387562 | 3.2:-26512424 | MS.gene067529:CDS |
| CTTACCTTTCTTTGTTGGGT+TGG | 0.393169 | 3.2:-26512372 | MS.gene067529:CDS |
| AATTTACCTCAAACACATAA+TGG | 0.412526 | 3.2:+26512045 | None:intergenic |
| TATTAAAGCTGGTTCGTTTG+CGG | 0.436341 | 3.2:-26512400 | MS.gene067529:CDS |
| TTTGTCTTTGCCGTTGTCTA+CGG | 0.443563 | 3.2:-26512444 | MS.gene067529:CDS |
| GTTCATATCCTATGGATGAT+TGG | 0.446343 | 3.2:+26511552 | None:intergenic |
| AGGATATGGTTCATATCCTA+TGG | 0.457257 | 3.2:+26511544 | None:intergenic |
| GACAGGGTGGATAAAATTAA+GGG | 0.468090 | 3.2:+26512485 | None:intergenic |
| GCTGCTTTGGATATTAAAGC+TGG | 0.472736 | 3.2:-26512411 | MS.gene067529:CDS |
| ATAGGATATGAACCATATCC+TGG | 0.473768 | 3.2:-26511542 | MS.gene067529:CDS |
| TATGATCTTGCAGGTTGTGT+TGG | 0.480273 | 3.2:-26512105 | MS.gene067529:intron |
| CCATTGGCTACGAACTCGCT+TGG | 0.488285 | 3.2:-26512329 | MS.gene067529:CDS |
| GAAAAGCACTTTGCCTTCTA+CGG | 0.495605 | 3.2:-26512621 | MS.gene067529:CDS |
| AGAACCAACCCAACAAAGAA+AGG | 0.516029 | 3.2:+26512368 | None:intergenic |
| GGTAAACAAGAGCAGTGAAA+AGG | 0.518223 | 3.2:+26512541 | None:intergenic |
| ACAAGAGCAGTGAAAAGGAT+TGG | 0.535729 | 3.2:+26512546 | None:intergenic |
| AAAATGGAGGAGTGAAGTAA+AGG | 0.535761 | 3.2:+26512520 | None:intergenic |
| TATCCAAAGCAGCGTAGAAG+AGG | 0.546806 | 3.2:+26512421 | None:intergenic |
| GCTGTGGTAAGAACCGTAGA+AGG | 0.548197 | 3.2:+26512608 | None:intergenic |
| TAGATGCTAATATCAAAGAA+TGG | 0.550042 | 3.2:-26511493 | MS.gene067529:CDS |
| TGGCTACGAACTCGCTTGGA+AGG | 0.554028 | 3.2:-26512325 | MS.gene067529:intron |
| TAGATCCTCCAATCATCCAT+AGG | 0.596680 | 3.2:-26511560 | MS.gene067529:CDS |
| CATATCCTATGGATGATTGG+AGG | 0.601474 | 3.2:+26511555 | None:intergenic |
| CCAAGCGAGTTCGTAGCCAA+TGG | 0.611989 | 3.2:+26512329 | None:intergenic |
| GTAGAAGAGGCCGTAGACAA+CGG | 0.626155 | 3.2:+26512434 | None:intergenic |
| TAAAGCTGGTTCGTTTGCGG+CGG | 0.626479 | 3.2:-26512397 | MS.gene067529:CDS |
| GCAATGTTGTCCAATAAAGC+TGG | 0.640581 | 3.2:+26511941 | None:intergenic |
| AAAGGATTGGCCAAACAAAG+AGG | 0.660028 | 3.2:+26512559 | None:intergenic |
| CAACGGCAAAGACAAAACCA+AGG | 0.747192 | 3.2:+26512451 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAGAAATTAATGCTTAATT+GGG | + | chr3.2:26511832-26511851 | None:intergenic | 15.0% |
| !! | AAGTTGAAATTATAATACAA+AGG | - | chr3.2:26512337-26512356 | MS.gene067529:CDS | 15.0% |
| !! | TAAAGAAATTAATGCTTAAT+TGG | + | chr3.2:26511833-26511852 | None:intergenic | 15.0% |
| !! | TGTATTATAATTTCAACTTA+GGG | + | chr3.2:26512336-26512355 | None:intergenic | 15.0% |
| !! | TTGTATTATAATTTCAACTT+AGG | + | chr3.2:26512337-26512356 | None:intergenic | 15.0% |
| !!! | TTTCTTTACATTTTATTTAG+AGG | - | chr3.2:26511869-26511888 | MS.gene067529:intron | 15.0% |
| !!! | ATGTGAATTATGATGTTTTT+TGG | + | chr3.2:26511523-26511542 | None:intergenic | 20.0% |
| !!! | TGTGAATTATGATGTTTTTT+GGG | + | chr3.2:26511522-26511541 | None:intergenic | 20.0% |
| ! | AAGACTAAAGATACGAAAAA+AGG | + | chr3.2:26512442-26512461 | None:intergenic | 25.0% |
| ! | AATTTACCTCAAACACATAA+TGG | + | chr3.2:26512060-26512079 | None:intergenic | 25.0% |
| ! | CATATAATAAAAATGCCATG+AGG | - | chr3.2:26511913-26511932 | MS.gene067529:CDS | 25.0% |
| ! | TAGATGCTAATATCAAAGAA+TGG | - | chr3.2:26512609-26512628 | MS.gene067529:CDS | 25.0% |
| ! | TTTCTTTCAAAACTGAAACA+AGG | - | chr3.2:26512497-26512516 | MS.gene067529:CDS | 25.0% |
| !! | ATTAAGTCACAACAGATTTT+TGG | + | chr3.2:26511967-26511986 | None:intergenic | 25.0% |
| !! | CTCATGGCATTTTTATTATA+TGG | + | chr3.2:26511915-26511934 | None:intergenic | 25.0% |
| AGGGCATAACAATGAAAAAA+TGG | + | chr3.2:26511601-26511620 | None:intergenic | 30.0% | |
| ATATCCTGGATTTCAGAAAA+AGG | - | chr3.2:26512574-26512593 | MS.gene067529:CDS | 30.0% | |
| ATTTATGACTATGATCTTGC+AGG | - | chr3.2:26511988-26512007 | MS.gene067529:intron | 30.0% | |
| CAAGGTTAAAAATCAAGACA+GGG | + | chr3.2:26511636-26511655 | None:intergenic | 30.0% | |
| CGAAAAAAGGTAGAAAATGA+AGG | + | chr3.2:26512429-26512448 | None:intergenic | 30.0% | |
| GCATAACAATGAAAAAATGG+AGG | + | chr3.2:26511598-26511617 | None:intergenic | 30.0% | |
| TAATTCACATCCTCTTTGTT+TGG | - | chr3.2:26511533-26511552 | MS.gene067529:CDS | 30.0% | |
| TCCAGAGTAATGTTGTAATA+TGG | - | chr3.2:26512275-26512294 | MS.gene067529:intron | 30.0% | |
| TCCATATTACAACATTACTC+TGG | + | chr3.2:26512279-26512298 | None:intergenic | 30.0% | |
| ! | ATAATTTTCGATATGCCTCA+TGG | + | chr3.2:26511931-26511950 | None:intergenic | 30.0% |
| !!! | AGTTTTGTTGCAAATTCCAT+TGG | - | chr3.2:26511757-26511776 | MS.gene067529:intron | 30.0% |
| !!! | TTTTTCTGAAATCCAGGATA+TGG | + | chr3.2:26512575-26512594 | None:intergenic | 30.0% |
| AAAATGGAGGAGTGAAGTAA+AGG | + | chr3.2:26511585-26511604 | None:intergenic | 35.0% | |
| ACTCATAGTTACCGATAAGT+GGG | - | chr3.2:26512248-26512267 | MS.gene067529:intron | 35.0% | |
| AGACAGGGTGGATAAAATTA+AGG | + | chr3.2:26511621-26511640 | None:intergenic | 35.0% | |
| AGGATATGGTTCATATCCTA+TGG | + | chr3.2:26512561-26512580 | None:intergenic | 35.0% | |
| ATAGGATATGAACCATATCC+TGG | - | chr3.2:26512560-26512579 | MS.gene067529:CDS | 35.0% | |
| CCAAGGTTAAAAATCAAGAC+AGG | + | chr3.2:26511637-26511656 | None:intergenic | 35.0% | |
| GACAGGGTGGATAAAATTAA+GGG | + | chr3.2:26511620-26511639 | None:intergenic | 35.0% | |
| GGTTCTTACCTTTCTTTGTT+GGG | - | chr3.2:26511726-26511745 | MS.gene067529:intron | 35.0% | |
| GTTCATATCCTATGGATGAT+TGG | + | chr3.2:26512553-26512572 | None:intergenic | 35.0% | |
| ! | CATTGCTCAAGCTTTTCTAA+TGG | - | chr3.2:26512178-26512197 | MS.gene067529:intron | 35.0% |
| ! | CCTGTCTTGATTTTTAACCT+TGG | - | chr3.2:26511634-26511653 | MS.gene067529:intron | 35.0% |
| ! | CGTATCTTTAGTCTTTGTAC+CGG | - | chr3.2:26512447-26512466 | MS.gene067529:CDS | 35.0% |
| ! | GTATCTTTAGTCTTTGTACC+GGG | - | chr3.2:26512448-26512467 | MS.gene067529:CDS | 35.0% |
| ! | TTTTGGCCATTATGTGTTTG+AGG | - | chr3.2:26512051-26512070 | MS.gene067529:intron | 35.0% |
| !! | TATTAAAGCTGGTTCGTTTG+CGG | - | chr3.2:26511702-26511721 | MS.gene067529:intron | 35.0% |
| !!! | GATGTCTCACATTTTTTTCG+CGG | - | chr3.2:26511806-26511825 | MS.gene067529:intron | 35.0% |
| ACAAGAGCAGTGAAAAGGAT+TGG | + | chr3.2:26511559-26511578 | None:intergenic | 40.0% | |
| ACCTCAAGAACCACGAAAAA+AGG | + | chr3.2:26512206-26512225 | None:intergenic | 40.0% | |
| AGAACCAACCCAACAAAGAA+AGG | + | chr3.2:26511737-26511756 | None:intergenic | 40.0% | |
| CATATCCTATGGATGATTGG+AGG | + | chr3.2:26512550-26512569 | None:intergenic | 40.0% | |
| CGGTTCTTACCTTTCTTTGT+TGG | - | chr3.2:26511725-26511744 | MS.gene067529:intron | 40.0% | |
| CTTACCTTTCTTTGTTGGGT+TGG | - | chr3.2:26511730-26511749 | MS.gene067529:intron | 40.0% | |
| GACTCATAGTTACCGATAAG+TGG | - | chr3.2:26512247-26512266 | MS.gene067529:intron | 40.0% | |
| GATCTGTTAGAACAGTATCC+CGG | + | chr3.2:26512469-26512488 | None:intergenic | 40.0% | |
| GCAATGTTGTCCAATAAAGC+TGG | + | chr3.2:26512164-26512183 | None:intergenic | 40.0% | |
| GGATACTGTTCTAACAGATC+TGG | - | chr3.2:26512469-26512488 | MS.gene067529:CDS | 40.0% | |
| GGTAAACAAGAGCAGTGAAA+AGG | + | chr3.2:26511564-26511583 | None:intergenic | 40.0% | |
| GGTTAAAAATCAAGACAGGG+TGG | + | chr3.2:26511633-26511652 | None:intergenic | 40.0% | |
| TAGATCCTCCAATCATCCAT+AGG | - | chr3.2:26512542-26512561 | MS.gene067529:CDS | 40.0% | |
| TATGATCTTGCAGGTTGTGT+TGG | - | chr3.2:26511997-26512016 | MS.gene067529:intron | 40.0% | |
| TTTGTCTTTGCCGTTGTCTA+CGG | - | chr3.2:26511658-26511677 | MS.gene067529:intron | 40.0% | |
| ! | AACTATAGTCGCTTTGCAGT+TGG | - | chr3.2:26512360-26512379 | MS.gene067529:CDS | 40.0% |
| ! | CGTATCATGCTTTTCAGTGA+TGG | + | chr3.2:26512089-26512108 | None:intergenic | 40.0% |
| ! | GAAAATGAAGGCACGATGTT+TGG | + | chr3.2:26512417-26512436 | None:intergenic | 40.0% |
| ! | GCTGCTTTGGATATTAAAGC+TGG | - | chr3.2:26511691-26511710 | MS.gene067529:intron | 40.0% |
| ! | TGGATTTCAGAAAAAGGTGC+AGG | - | chr3.2:26512580-26512599 | MS.gene067529:CDS | 40.0% |
| !! | AAAGGATTGGCCAAACAAAG+AGG | + | chr3.2:26511546-26511565 | None:intergenic | 40.0% |
| !! | GAAAAGCACTTTGCCTTCTA+CGG | - | chr3.2:26511481-26511500 | MS.gene067529:CDS | 40.0% |
| !! | GGCTACTCAGTTGTTTTGTT+GGG | - | chr3.2:26512018-26512037 | MS.gene067529:intron | 40.0% |
| !! | TGGCTACTCAGTTGTTTTGT+TGG | - | chr3.2:26512017-26512036 | MS.gene067529:intron | 40.0% |
| !!! | TCCTTTTTTCGTGGTTCTTG+AGG | - | chr3.2:26512202-26512221 | MS.gene067529:intron | 40.0% |
| !!! | TCTAATGGCTCCTTTTTTCG+TGG | - | chr3.2:26512193-26512212 | MS.gene067529:intron | 40.0% |
| !!! | TGATGTTTTTTGGGTTGCTG+TGG | + | chr3.2:26511513-26511532 | None:intergenic | 40.0% |
| !!! | TGCACCTTTTTCTGAAATCC+AGG | + | chr3.2:26512581-26512600 | None:intergenic | 40.0% |
| CAACGGCAAAGACAAAACCA+AGG | + | chr3.2:26511654-26511673 | None:intergenic | 45.0% | |
| CATAGTTACCGATAAGTGGG+TGG | - | chr3.2:26512251-26512270 | MS.gene067529:intron | 45.0% | |
| TATCCAAAGCAGCGTAGAAG+AGG | + | chr3.2:26511684-26511703 | None:intergenic | 45.0% | |
| ! | GAAACGTGCACCAGCTTTAT+TGG | - | chr3.2:26512151-26512170 | MS.gene067529:intron | 45.0% |
| ! | TGTTGGGCAGGACAGATTTT+TGG | - | chr3.2:26512034-26512053 | MS.gene067529:intron | 45.0% |
| ! | TTCAGAAAAAGGTGCAGGCA+AGG | - | chr3.2:26512585-26512604 | MS.gene067529:CDS | 45.0% |
| !! | ACTCAGTTGTTTTGTTGGGC+AGG | - | chr3.2:26512022-26512041 | MS.gene067529:intron | 45.0% |
| CGCTTTGCAGTTGGATACGT+AGG | - | chr3.2:26512369-26512388 | MS.gene067529:CDS | 50.0% | |
| GCTGTGGTAAGAACCGTAGA+AGG | + | chr3.2:26511497-26511516 | None:intergenic | 50.0% | |
| GTAGAAGAGGCCGTAGACAA+CGG | + | chr3.2:26511671-26511690 | None:intergenic | 50.0% | |
| !! | CTCTGGAACCACCCACTTAT+CGG | + | chr3.2:26512262-26512281 | None:intergenic | 50.0% |
| !! | TAAAGCTGGTTCGTTTGCGG+CGG | - | chr3.2:26511705-26511724 | MS.gene067529:intron | 50.0% |
| CCAAGCGAGTTCGTAGCCAA+TGG | + | chr3.2:26511776-26511795 | None:intergenic | 55.0% | |
| TGGCTACGAACTCGCTTGGA+AGG | - | chr3.2:26511777-26511796 | MS.gene067529:intron | 55.0% | |
| ! | CCATTGGCTACGAACTCGCT+TGG | - | chr3.2:26511773-26511792 | MS.gene067529:intron | 55.0% |
| CGGCCTCTTCTACGCTGCTT+TGG | - | chr3.2:26511678-26511697 | MS.gene067529:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 26511463 | 26512661 | 26511463 | ID=MS.gene067529 |
| chr3.2 | mRNA | 26511463 | 26512661 | 26511463 | ID=MS.gene067529.t1;Parent=MS.gene067529 |
| chr3.2 | exon | 26512326 | 26512661 | 26512326 | ID=MS.gene067529.t1.exon1;Parent=MS.gene067529.t1 |
| chr3.2 | CDS | 26512326 | 26512661 | 26512326 | ID=cds.MS.gene067529.t1;Parent=MS.gene067529.t1 |
| chr3.2 | exon | 26512052 | 26512114 | 26512052 | ID=MS.gene067529.t1.exon2;Parent=MS.gene067529.t1 |
| chr3.2 | CDS | 26512052 | 26512114 | 26512052 | ID=cds.MS.gene067529.t1;Parent=MS.gene067529.t1 |
| chr3.2 | exon | 26511901 | 26511972 | 26511901 | ID=MS.gene067529.t1.exon3;Parent=MS.gene067529.t1 |
| chr3.2 | CDS | 26511901 | 26511972 | 26511901 | ID=cds.MS.gene067529.t1;Parent=MS.gene067529.t1 |
| chr3.2 | exon | 26511463 | 26511579 | 26511463 | ID=MS.gene067529.t1.exon4;Parent=MS.gene067529.t1 |
| chr3.2 | CDS | 26511463 | 26511579 | 26511463 | ID=cds.MS.gene067529.t1;Parent=MS.gene067529.t1 |
| Gene Sequence |
| Protein sequence |
