AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene01960

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene01960.t1 MTR_2g027390 96.759 432 10 2 1 428 1 432 0.0 842
MS.gene01960.t1 MTR_7g017500 39.474 114 66 3 93 205 149 260 1.61e-22 100
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene01960.t1 AT5G47790 58.508 429 116 8 1 428 1 368 6.70e-162 461
MS.gene01960.t1 AT5G38840 39.640 111 64 3 96 205 93 201 3.82e-19 90.5

Find 125 sgRNAs with CRISPR-Local

Find 153 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGTGTATATGTAATTGATT+TGG 0.192768 2.2:-59859827 MS.gene01960:CDS
TTGGAACCGTTCTCTGTTAA+TGG 0.193902 2.2:-59860631 MS.gene01960:CDS
GGTGCTAGATAGGATTAATT+TGG 0.279458 2.2:-59860332 MS.gene01960:CDS
AGAAGATGAGAGTTTCATTC+AGG 0.291478 2.2:-59859465 MS.gene01960:CDS
AGTGGAGGCGGCTCCGTTGT+TGG 0.292771 2.2:-59860470 MS.gene01960:CDS
GTGGAGGCGGCTCCGTTGTT+GGG 0.315269 2.2:-59860469 MS.gene01960:CDS
GTTTAAGAAAGCCGAGAGTT+TGG 0.325196 2.2:-59860650 MS.gene01960:CDS
ACTACCAAGCTCAGTGGATT+TGG 0.351010 2.2:+59859543 None:intergenic
GGGATTTACGATGACCTATA+TGG 0.356648 2.2:-59859196 MS.gene01960:CDS
GAGGACTGCCCTTTCCCCTT+TGG 0.357778 2.2:+59859304 None:intergenic
TTTCATTCAGGGATCAGGTT+GGG 0.361537 2.2:-59859453 MS.gene01960:CDS
AGTGTATATGTAATTGATTT+GGG 0.362845 2.2:-59859826 MS.gene01960:CDS
GAGGATGATGATGATTTGTT+TGG 0.370753 2.2:-59859043 MS.gene01960:CDS
CCCTCGTCCTTCACCACCCA+CGG 0.373388 2.2:-59859668 MS.gene01960:CDS
CTTCCTTCCTTAACACCAAC+TGG 0.386824 2.2:+59859370 None:intergenic
GAAGAACATCTGGTCTTGAT+AGG 0.392084 2.2:-59860672 MS.gene01960:CDS
ACCTGGTCCAGTTGGTGTTA+AGG 0.396500 2.2:-59859377 MS.gene01960:CDS
TTCATTCAGGGATCAGGTTG+GGG 0.397217 2.2:-59859452 MS.gene01960:CDS
CGCGGCTCAATCGCCCAATC+TGG 0.397896 2.2:+59860394 None:intergenic
TGTAATTTGAAGAGAATGTA+TGG 0.407152 2.2:-59860694 None:intergenic
GGAGAGTTAGTTGAGGTTGT+TGG 0.408959 2.2:-59859430 MS.gene01960:CDS
TAAGGTAGTTGGTCATTCTT+CGG 0.414880 2.2:-59860572 MS.gene01960:CDS
TCTTAGCAGCTCTTTCTGAT+TGG 0.422101 2.2:+59859494 None:intergenic
TCTCCATTCTCCTTAGCTGC+TGG 0.430224 2.2:+59859106 None:intergenic
GTTGTTGGGGCAGAATCAAC+AGG 0.433225 2.2:-59860455 MS.gene01960:CDS
GAGAGTTTCATTCAGGGATC+AGG 0.440722 2.2:-59859458 MS.gene01960:CDS
AGGATGGTCAGGTGCTAGAT+AGG 0.444081 2.2:-59860342 MS.gene01960:CDS
GATGTGGCAGCCACCAGATT+GGG 0.444291 2.2:-59860407 MS.gene01960:CDS
TCTGATGGGTCAGGAGGTGG+TGG 0.444611 2.2:+59859634 None:intergenic
AATACGCTGCTGAATCGTTA+TGG 0.447922 2.2:-59859589 MS.gene01960:CDS
CAAGCACAAAATCACAAGTC+TGG 0.448208 2.2:+59860278 None:intergenic
AGAATGTATGGAAGAACATC+TGG 0.453804 2.2:-59860682 MS.gene01960:CDS
TCATCTGATGGGTCAGGAGG+TGG 0.454291 2.2:+59859631 None:intergenic
GCCTCTTCATCTGATGGGTC+AGG 0.463310 2.2:+59859625 None:intergenic
CTGTAATTCCAAAGGGGAAA+GGG 0.464451 2.2:-59859312 MS.gene01960:CDS
CAAACTACTGTAATTCCAAA+GGG 0.471274 2.2:-59859319 MS.gene01960:CDS
AAACTCAACAGGTGAATCCT+TGG 0.474394 2.2:+59859765 None:intergenic
CGATGTGGCAGCCACCAGAT+TGG 0.476461 2.2:-59860408 MS.gene01960:CDS
TCCTTAACACCAACTGGACC+AGG 0.478092 2.2:+59859376 None:intergenic
GCGCCAGCAGCTAAGGAGAA+TGG 0.478653 2.2:-59859109 MS.gene01960:CDS
CCTCGTCCTTCACCACCCAC+GGG 0.483891 2.2:-59859667 MS.gene01960:CDS
TGTCCCACTTCAAACTCAAC+AGG 0.484199 2.2:+59859754 None:intergenic
CTGTCAAAGTTGGATCAGCC+TGG 0.486875 2.2:-59859162 MS.gene01960:CDS
TTGATCCCCGTGGGTGGTGA+AGG 0.486979 2.2:+59859661 None:intergenic
AGGATGATGATGATTTGTTT+GGG 0.488049 2.2:-59859042 MS.gene01960:CDS
TCCTCGTAATCAGCAATGCT+TGG 0.489223 2.2:+59859061 None:intergenic
GAATCATTATCTGTCAAAGT+TGG 0.489351 2.2:-59859172 MS.gene01960:CDS
TGTACAGAATTCTTCGACTG+TGG 0.489814 2.2:+59860538 None:intergenic
CAGATAATGATTCTCCATAT+AGG 0.494590 2.2:+59859182 None:intergenic
GGAAGTTATGAAGGATGGTC+AGG 0.495075 2.2:-59860353 MS.gene01960:CDS
TGGAGGCGGCTCCGTTGTTG+GGG 0.498085 2.2:-59860468 MS.gene01960:CDS
AATCAACAGGCAACTCAGAT+TGG 0.499106 2.2:-59860442 MS.gene01960:CDS
TTGGGCGATTGAGCCGCGAC+CGG 0.501004 2.2:-59860389 MS.gene01960:CDS
GGAAAGGGCAGTCCTCTGCT+AGG 0.502686 2.2:-59859297 MS.gene01960:CDS
TACTTGGAAGTTATGAAGGA+TGG 0.503632 2.2:-59860358 MS.gene01960:CDS
GAGGTAGATTCATCCCAAAA+AGG 0.506021 2.2:-59859274 MS.gene01960:CDS
TTCTCAGAAAGGTGTGGGAG+TGG 0.506673 2.2:-59860488 MS.gene01960:CDS
TGTTGCTCAGTCTTCTAGTA+AGG 0.506725 2.2:-59860590 MS.gene01960:CDS
GAAGATGAGAGTTTCATTCA+GGG 0.508142 2.2:-59859464 MS.gene01960:CDS
GAAAGGTGTGGGAGTGGAGG+CGG 0.508188 2.2:-59860482 MS.gene01960:CDS
GTTAAGGAAGGAAGTCTAGT+TGG 0.508857 2.2:-59859361 MS.gene01960:CDS
TGGGCGATTGAGCCGCGACC+GGG 0.511260 2.2:-59860388 MS.gene01960:CDS
TAGTTGGTCATTCTTCGGCT+TGG 0.516977 2.2:-59860567 MS.gene01960:CDS
TGAACGAGCGCCAGCAGCTA+AGG 0.523991 2.2:-59859116 MS.gene01960:CDS
CATCAACATGGTTCTCAGAA+AGG 0.526028 2.2:-59860499 MS.gene01960:CDS
CAGTCTTCTAGTAAGGTAGT+TGG 0.528464 2.2:-59860583 MS.gene01960:CDS
TCCTGACCCATCAGATGAAG+AGG 0.533497 2.2:-59859626 MS.gene01960:CDS
GCTAAGGAGAATGGAGAAAG+TGG 0.536680 2.2:-59859100 MS.gene01960:CDS
CTCTCGCCAACATGCTGCGG+TGG 0.540409 2.2:-59860242 MS.gene01960:CDS
GGCTCAATCGCCCAATCTGG+TGG 0.540495 2.2:+59860397 None:intergenic
ACAACAGCCTCTTCATCTGA+TGG 0.540627 2.2:+59859619 None:intergenic
CCCGTGGGTGGTGAAGGACG+AGG 0.543398 2.2:+59859667 None:intergenic
TCTTAATATGTATAATCTCG+TGG 0.545734 2.2:+59859711 None:intergenic
GAGCTTGGTAGTGCAACAAA+TGG 0.548432 2.2:-59859532 MS.gene01960:CDS
GTTTCATTCAGGGATCAGGT+TGG 0.560524 2.2:-59859454 MS.gene01960:CDS
GAAAGGGCAGTCCTCTGCTA+GGG 0.561223 2.2:-59859296 MS.gene01960:CDS
GCTCGTTCACCACTACTGAC+AGG 0.562972 2.2:+59859130 None:intergenic
CAACAGGCAACTCAGATTGG+TGG 0.563642 2.2:-59860439 MS.gene01960:CDS
CAAACGCTCATTTGCAACAA+AGG 0.565573 2.2:+59859789 None:intergenic
TTCACCTGTTGAGTTTGAAG+TGG 0.567231 2.2:-59859758 MS.gene01960:CDS
GGGAAATTGATCCCCGTGGG+TGG 0.570676 2.2:+59859655 None:intergenic
TCACCTGTTGAGTTTGAAGT+GGG 0.574165 2.2:-59859757 MS.gene01960:CDS
GAGGTTGTTGGAATTTCAGA+TGG 0.576473 2.2:-59859418 MS.gene01960:CDS
CAGGCAACTCAGATTGGTGG+AGG 0.577255 2.2:-59860436 MS.gene01960:CDS
TCTTCATCTGATGGGTCAGG+AGG 0.578491 2.2:+59859628 None:intergenic
CATGGTTCTCAGAAAGGTGT+GGG 0.580915 2.2:-59860493 MS.gene01960:CDS
TGTCTCTCGCCAACATGCTG+CGG 0.581927 2.2:-59860245 MS.gene01960:CDS
ACAAACTACTGTAATTCCAA+AGG 0.585279 2.2:-59859320 MS.gene01960:CDS
GAATTACCATTAACAGAGAA+CGG 0.587204 2.2:+59860625 None:intergenic
TCTAACCTTCCGTTCTTGTG+AGG 0.588526 2.2:+59860217 None:intergenic
ACTGTAATTCCAAAGGGGAA+AGG 0.588891 2.2:-59859313 MS.gene01960:CDS
AGAGAACGGTTCCAAACTCT+CGG 0.589404 2.2:+59860639 None:intergenic
TGTCAAAGTTGGATCAGCCT+GGG 0.595464 2.2:-59859161 MS.gene01960:CDS
CAACAGCCTCTTCATCTGAT+GGG 0.598150 2.2:+59859620 None:intergenic
AGGGCAGTCCTCTGCTAGGG+AGG 0.602420 2.2:-59859293 MS.gene01960:CDS
GAGGAACCACCGCAGCATGT+TGG 0.602912 2.2:+59860236 None:intergenic
TGGTGGGAAATTGATCCCCG+TGG 0.605958 2.2:+59859651 None:intergenic
TCAGAAAGGTGTGGGAGTGG+AGG 0.607307 2.2:-59860485 MS.gene01960:CDS
TGCAAATGAGCGTTTGACCA+AGG 0.607826 2.2:-59859782 MS.gene01960:CDS
GGTCCAGTTGGTGTTAAGGA+AGG 0.608986 2.2:-59859373 MS.gene01960:CDS
GAAACAGAACCTGGTCCAGT+TGG 0.609238 2.2:-59859385 MS.gene01960:CDS
ACATGGTTCTCAGAAAGGTG+TGG 0.612081 2.2:-59860494 MS.gene01960:CDS
GTGTCCAAATCCACTGAGCT+TGG 0.614575 2.2:-59859547 MS.gene01960:CDS
GCGGTGGTTCCTCACAAGAA+CGG 0.615525 2.2:-59860226 MS.gene01960:CDS
ACTGACAGGTGAGTATGCCC+AGG 0.616123 2.2:+59859144 None:intergenic
TCATTCAGGGATCAGGTTGG+GGG 0.620615 2.2:-59859451 MS.gene01960:CDS
TGTTGATTCTGCCCCAACAA+CGG 0.623282 2.2:+59860457 None:intergenic
GCATACTCACCTGTCAGTAG+TGG 0.625393 2.2:-59859139 MS.gene01960:CDS
TGAATCTACCTCCCTAGCAG+AGG 0.627223 2.2:+59859285 None:intergenic
CCGTGGGTGGTGAAGGACGA+GGG 0.627786 2.2:+59859668 None:intergenic
TCCAAGCATTGCTGATTACG+AGG 0.635210 2.2:-59859062 MS.gene01960:CDS
TTGGTGGAGGGCAGTCGATG+TGG 0.637100 2.2:-59860423 MS.gene01960:CDS
ACAGAATTCTTCGACTGTGG+AGG 0.638226 2.2:+59860541 None:intergenic
TGGTTCCTCACAAGAACGGA+AGG 0.642477 2.2:-59860222 MS.gene01960:intron
GGTGGGAAATTGATCCCCGT+GGG 0.643525 2.2:+59859652 None:intergenic
CTGATGGGTCAGGAGGTGGT+GGG 0.647207 2.2:+59859635 None:intergenic
TGTTGCACTACCAAGCTCAG+TGG 0.656951 2.2:+59859537 None:intergenic
TTGAAGTGGGACAATCATTG+CGG 0.662047 2.2:-59859744 MS.gene01960:CDS
ATTGATTTGGGATCTGCACA+TGG 0.667462 2.2:-59859814 MS.gene01960:CDS
CTCGTCCTTCACCACCCACG+GGG 0.671541 2.2:-59859666 MS.gene01960:CDS
AGGCAACTCAGATTGGTGGA+GGG 0.676455 2.2:-59860435 MS.gene01960:CDS
TCACAGAATCAACATCAACA+TGG 0.707104 2.2:-59860511 MS.gene01960:CDS
AAACTACTGTAATTCCAAAG+GGG 0.709535 2.2:-59859318 MS.gene01960:CDS
GTAGATGTAGAAACAGAACC+TGG 0.729040 2.2:-59859394 MS.gene01960:CDS
TGAAGGACGAGGGAAAAGAG+CGG 0.744449 2.2:+59859678 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATATGTCTTATATTATTT+AGG - chr2.2:59859817-59859836 MS.gene01960:CDS 10.0%
!!! ATTATTTAGAATATGCAATT+CGG + chr2.2:59859599-59859618 None:intergenic 15.0%
!! AAATGAATTACAAATGACTT+AGG - chr2.2:59859755-59859774 MS.gene01960:CDS 20.0%
!!! AGTGTATATGTAATTGATTT+GGG - chr2.2:59859892-59859911 MS.gene01960:intron 20.0%
!!! ATAGACGAAGAAATATTTTT+GGG - chr2.2:59859409-59859428 MS.gene01960:CDS 20.0%
! ATTACAACTATAAGTTGCAA+GGG + chr2.2:59859853-59859872 None:intergenic 25.0%
! CAGTGTATATGTAATTGATT+TGG - chr2.2:59859891-59859910 MS.gene01960:intron 25.0%
! TATTACAACTATAAGTTGCA+AGG + chr2.2:59859854-59859873 None:intergenic 25.0%
! TCTTAATATGTATAATCTCG+TGG + chr2.2:59860010-59860029 None:intergenic 25.0%
! TTTCGTTTGATAAGGTAATA+TGG + chr2.2:59859694-59859713 None:intergenic 25.0%
!! GATAGACGAAGAAATATTTT+TGG - chr2.2:59859408-59859427 MS.gene01960:CDS 25.0%
!! TTTTTACTTGGAAGTTATGA+AGG - chr2.2:59859356-59859375 MS.gene01960:CDS 25.0%
AAACTACTGTAATTCCAAAG+GGG - chr2.2:59860400-59860419 MS.gene01960:CDS 30.0%
ACAAACTACTGTAATTCCAA+AGG - chr2.2:59860398-59860417 MS.gene01960:CDS 30.0%
CAAACTACTGTAATTCCAAA+GGG - chr2.2:59860399-59860418 MS.gene01960:CDS 30.0%
CAGATAATGATTCTCCATAT+AGG + chr2.2:59860539-59860558 None:intergenic 30.0%
GAATCATTATCTGTCAAAGT+TGG - chr2.2:59860546-59860565 MS.gene01960:CDS 30.0%
GAATTACCATTAACAGAGAA+CGG + chr2.2:59859096-59859115 None:intergenic 30.0%
TTGAATGCTTTCGTTTGATA+AGG + chr2.2:59859702-59859721 None:intergenic 30.0%
! TTTATCAGTCACACCTTTTT+GGG + chr2.2:59860460-59860479 None:intergenic 30.0%
!! AATATGGTGTCATTTGATAC+AGG + chr2.2:59859678-59859697 None:intergenic 30.0%
!! AGGATGATGATGATTTGTTT+GGG - chr2.2:59860676-59860695 MS.gene01960:CDS 30.0%
ACTTCCAAGTAAAAAACACC+CGG + chr2.2:59859351-59859370 None:intergenic 35.0%
AGAAGATGAGAGTTTCATTC+AGG - chr2.2:59860253-59860272 MS.gene01960:CDS 35.0%
AGGTAATAGATGCAAGCTAT+TGG + chr2.2:59859658-59859677 None:intergenic 35.0%
ATCCATTCCAGTAACATCAA+AGG + chr2.2:59859541-59859560 None:intergenic 35.0%
GAAGATGAGAGTTTCATTCA+GGG - chr2.2:59860254-59860273 MS.gene01960:CDS 35.0%
GGTGCTAGATAGGATTAATT+TGG - chr2.2:59859386-59859405 MS.gene01960:CDS 35.0%
TAAGGTAGTTGGTCATTCTT+CGG - chr2.2:59859146-59859165 MS.gene01960:CDS 35.0%
TACTTGGAAGTTATGAAGGA+TGG - chr2.2:59859360-59859379 MS.gene01960:CDS 35.0%
TCACAGAATCAACATCAACA+TGG - chr2.2:59859207-59859226 MS.gene01960:CDS 35.0%
TGCAACAAATGGCAAAAACA+AGG - chr2.2:59860197-59860216 MS.gene01960:intron 35.0%
! GTTTATCAGTCACACCTTTT+TGG + chr2.2:59860461-59860480 None:intergenic 35.0%
!! AAAAGCTAAAAGTGCTGAAG+TGG + chr2.2:59859783-59859802 None:intergenic 35.0%
!! ATCGTAAATCCCAGTTTTAG+CGG + chr2.2:59860514-59860533 None:intergenic 35.0%
!! GAGGATGATGATGATTTGTT+TGG - chr2.2:59860675-59860694 MS.gene01960:CDS 35.0%
!!! GACGAAGAAATATTTTTGGG+AGG - chr2.2:59859412-59859431 MS.gene01960:CDS 35.0%
AAACTCAACAGGTGAATCCT+TGG + chr2.2:59859956-59859975 None:intergenic 40.0%
AATACGCTGCTGAATCGTTA+TGG - chr2.2:59860129-59860148 MS.gene01960:intron 40.0%
AATCAACAGGCAACTCAGAT+TGG - chr2.2:59859276-59859295 MS.gene01960:CDS 40.0%
ACTGTAATTCCAAAGGGGAA+AGG - chr2.2:59860405-59860424 MS.gene01960:CDS 40.0%
ATCAAAAACCCCGCTAAAAC+TGG - chr2.2:59860501-59860520 MS.gene01960:CDS 40.0%
CAAACGCTCATTTGCAACAA+AGG + chr2.2:59859932-59859951 None:intergenic 40.0%
CAAGCACAAAATCACAAGTC+TGG + chr2.2:59859443-59859462 None:intergenic 40.0%
CAGTCTTCTAGTAAGGTAGT+TGG - chr2.2:59859135-59859154 MS.gene01960:CDS 40.0%
CATCAACATGGTTCTCAGAA+AGG - chr2.2:59859219-59859238 MS.gene01960:CDS 40.0%
CTCCTTTGATGTTACTGGAA+TGG - chr2.2:59859536-59859555 MS.gene01960:CDS 40.0%
CTGTAATTCCAAAGGGGAAA+GGG - chr2.2:59860406-59860425 MS.gene01960:CDS 40.0%
GAGGTAGATTCATCCCAAAA+AGG - chr2.2:59860444-59860463 MS.gene01960:CDS 40.0%
GAGGTTGTTGGAATTTCAGA+TGG - chr2.2:59860300-59860319 MS.gene01960:CDS 40.0%
GGGATTTACGATGACCTATA+TGG - chr2.2:59860522-59860541 MS.gene01960:CDS 40.0%
GTAGATGTAGAAACAGAACC+TGG - chr2.2:59860324-59860343 MS.gene01960:CDS 40.0%
GTTAAGGAAGGAAGTCTAGT+TGG - chr2.2:59860357-59860376 MS.gene01960:CDS 40.0%
GTTTAAGAAAGCCGAGAGTT+TGG - chr2.2:59859068-59859087 MS.gene01960:CDS 40.0%
TCAAAAACCCCGCTAAAACT+GGG - chr2.2:59860502-59860521 MS.gene01960:CDS 40.0%
TCTCTCTCCTTTGATGTTAC+TGG - chr2.2:59859531-59859550 MS.gene01960:CDS 40.0%
TCTTAGCAGCTCTTTCTGAT+TGG + chr2.2:59860227-59860246 None:intergenic 40.0%
TGTACAGAATTCTTCGACTG+TGG + chr2.2:59859183-59859202 None:intergenic 40.0%
TGTTGCTCAGTCTTCTAGTA+AGG - chr2.2:59859128-59859147 MS.gene01960:CDS 40.0%
TTCACCTGTTGAGTTTGAAG+TGG - chr2.2:59859960-59859979 MS.gene01960:intron 40.0%
TTGAAGTGGGACAATCATTG+CGG - chr2.2:59859974-59859993 MS.gene01960:intron 40.0%
TTGGAACCGTTCTCTGTTAA+TGG - chr2.2:59859087-59859106 MS.gene01960:CDS 40.0%
TTTCATTCAGGGATCAGGTT+GGG - chr2.2:59860265-59860284 MS.gene01960:CDS 40.0%
! TCACCTGTTGAGTTTGAAGT+GGG - chr2.2:59859961-59859980 MS.gene01960:intron 40.0%
!! ATTGATTTGGGATCTGCACA+TGG - chr2.2:59859904-59859923 MS.gene01960:intron 40.0%
!! CGGGGTTTTTGATCTTGTTT+AGG + chr2.2:59860494-59860513 None:intergenic 40.0%
!! GAAGAACATCTGGTCTTGAT+AGG - chr2.2:59859046-59859065 MS.gene01960:CDS 40.0%
!! TCGTAAATCCCAGTTTTAGC+GGG + chr2.2:59860513-59860532 None:intergenic 40.0%
AAGTAAAAAACACCCGGTCG+CGG + chr2.2:59859345-59859364 None:intergenic 45.0%
ACAACAGCCTCTTCATCTGA+TGG + chr2.2:59860102-59860121 None:intergenic 45.0%
ACAGAATTCTTCGACTGTGG+AGG + chr2.2:59859180-59859199 None:intergenic 45.0%
ACATGGTTCTCAGAAAGGTG+TGG - chr2.2:59859224-59859243 MS.gene01960:CDS 45.0%
AGAGAACGGTTCCAAACTCT+CGG + chr2.2:59859082-59859101 None:intergenic 45.0%
CAACAGCCTCTTCATCTGAT+GGG + chr2.2:59860101-59860120 None:intergenic 45.0%
CTTCCTTCCTTAACACCAAC+TGG + chr2.2:59860351-59860370 None:intergenic 45.0%
GAGAGTTTCATTCAGGGATC+AGG - chr2.2:59860260-59860279 MS.gene01960:CDS 45.0%
GCTAAGGAGAATGGAGAAAG+TGG - chr2.2:59860618-59860637 MS.gene01960:CDS 45.0%
GGAAGTTATGAAGGATGGTC+AGG - chr2.2:59859365-59859384 MS.gene01960:CDS 45.0%
GGAGAGTTAGTTGAGGTTGT+TGG - chr2.2:59860288-59860307 MS.gene01960:CDS 45.0%
GTTTCATTCAGGGATCAGGT+TGG - chr2.2:59860264-59860283 MS.gene01960:CDS 45.0%
TAGTTGGTCATTCTTCGGCT+TGG - chr2.2:59859151-59859170 MS.gene01960:CDS 45.0%
TCCTCGTAATCAGCAATGCT+TGG + chr2.2:59860660-59860679 None:intergenic 45.0%
TCTAACCTTCCGTTCTTGTG+AGG + chr2.2:59859504-59859523 None:intergenic 45.0%
TGCAAATGAGCGTTTGACCA+AGG - chr2.2:59859936-59859955 MS.gene01960:intron 45.0%
TGTCAAAGTTGGATCAGCCT+GGG - chr2.2:59860557-59860576 MS.gene01960:CDS 45.0%
TGTCCCACTTCAAACTCAAC+AGG + chr2.2:59859967-59859986 None:intergenic 45.0%
! ACTACCAAGCTCAGTGGATT+TGG + chr2.2:59860178-59860197 None:intergenic 45.0%
! CATGGTTCTCAGAAAGGTGT+GGG - chr2.2:59859225-59859244 MS.gene01960:CDS 45.0%
! TCCAAGCATTGCTGATTACG+AGG - chr2.2:59860656-59860675 MS.gene01960:CDS 45.0%
! TTCATTCAGGGATCAGGTTG+GGG - chr2.2:59860266-59860285 MS.gene01960:CDS 45.0%
!! CGTAAATCCCAGTTTTAGCG+GGG + chr2.2:59860512-59860531 None:intergenic 45.0%
!! GAGCTTGGTAGTGCAACAAA+TGG - chr2.2:59860186-59860205 MS.gene01960:intron 45.0%
!! TGTTGATTCTGCCCCAACAA+CGG + chr2.2:59859264-59859283 None:intergenic 45.0%
AGGATGGTCAGGTGCTAGAT+AGG - chr2.2:59859376-59859395 MS.gene01960:CDS 50.0%
AGGCAACTCAGATTGGTGGA+GGG - chr2.2:59859283-59859302 MS.gene01960:CDS 50.0%
CAACAGGCAACTCAGATTGG+TGG - chr2.2:59859279-59859298 MS.gene01960:CDS 50.0%
CTGTCAAAGTTGGATCAGCC+TGG - chr2.2:59860556-59860575 MS.gene01960:CDS 50.0%
GAAACAGAACCTGGTCCAGT+TGG - chr2.2:59860333-59860352 MS.gene01960:CDS 50.0%
GCATACTCACCTGTCAGTAG+TGG - chr2.2:59860579-59860598 MS.gene01960:CDS 50.0%
GTGTCCAAATCCACTGAGCT+TGG - chr2.2:59860171-59860190 MS.gene01960:intron 50.0%
GTTGTTGGGGCAGAATCAAC+AGG - chr2.2:59859263-59859282 MS.gene01960:CDS 50.0%
TCCTGACCCATCAGATGAAG+AGG - chr2.2:59860092-59860111 MS.gene01960:intron 50.0%
TCCTTAACACCAACTGGACC+AGG + chr2.2:59860345-59860364 None:intergenic 50.0%
TCTCCATTCTCCTTAGCTGC+TGG + chr2.2:59860615-59860634 None:intergenic 50.0%
TGAAGGACGAGGGAAAAGAG+CGG + chr2.2:59860043-59860062 None:intergenic 50.0%
TGAATCTACCTCCCTAGCAG+AGG + chr2.2:59860436-59860455 None:intergenic 50.0%
TGGTTCCTCACAAGAACGGA+AGG - chr2.2:59859496-59859515 MS.gene01960:CDS 50.0%
TGTTGCACTACCAAGCTCAG+TGG + chr2.2:59860184-59860203 None:intergenic 50.0%
! TCTTCATCTGATGGGTCAGG+AGG + chr2.2:59860093-59860112 None:intergenic 50.0%
! TTCTCAGAAAGGTGTGGGAG+TGG - chr2.2:59859230-59859249 MS.gene01960:CDS 50.0%
!! ACCTGGTCCAGTTGGTGTTA+AGG - chr2.2:59860341-59860360 MS.gene01960:CDS 50.0%
!! GGTCCAGTTGGTGTTAAGGA+AGG - chr2.2:59860345-59860364 MS.gene01960:CDS 50.0%
!! TCATTCAGGGATCAGGTTGG+GGG - chr2.2:59860267-59860286 MS.gene01960:CDS 50.0%
!!! GCGACCGGGTGTTTTTTACT+TGG - chr2.2:59859344-59859363 MS.gene01960:CDS 50.0%
ACTGACAGGTGAGTATGCCC+AGG + chr2.2:59860577-59860596 None:intergenic 55.0%
CAGGCAACTCAGATTGGTGG+AGG - chr2.2:59859282-59859301 MS.gene01960:CDS 55.0%
GAAAGGGCAGTCCTCTGCTA+GGG - chr2.2:59860422-59860441 MS.gene01960:CDS 55.0%
GATGTGGCAGCCACCAGATT+GGG - chr2.2:59859311-59859330 MS.gene01960:CDS 55.0%
GCGGTGGTTCCTCACAAGAA+CGG - chr2.2:59859492-59859511 MS.gene01960:CDS 55.0%
GCTCGTTCACCACTACTGAC+AGG + chr2.2:59860591-59860610 None:intergenic 55.0%
GGTGGGAAATTGATCCCCGT+GGG + chr2.2:59860069-59860088 None:intergenic 55.0%
GGTTGGGGGAGAGTTAGTTG+AGG - chr2.2:59860281-59860300 MS.gene01960:CDS 55.0%
TGGTGGGAAATTGATCCCCG+TGG + chr2.2:59860070-59860089 None:intergenic 55.0%
TGTCTCTCGCCAACATGCTG+CGG - chr2.2:59859473-59859492 MS.gene01960:CDS 55.0%
! GCCTCTTCATCTGATGGGTC+AGG + chr2.2:59860096-59860115 None:intergenic 55.0%
! TCAGAAAGGTGTGGGAGTGG+AGG - chr2.2:59859233-59859252 MS.gene01960:CDS 55.0%
! TCATCTGATGGGTCAGGAGG+TGG + chr2.2:59860090-59860109 None:intergenic 55.0%
CGATGTGGCAGCCACCAGAT+TGG - chr2.2:59859310-59859329 MS.gene01960:CDS 60.0%
GAGGAACCACCGCAGCATGT+TGG + chr2.2:59859485-59859504 None:intergenic 60.0%
GAGGACTGCCCTTTCCCCTT+TGG + chr2.2:59860417-59860436 None:intergenic 60.0%
GCGCCAGCAGCTAAGGAGAA+TGG - chr2.2:59860609-59860628 MS.gene01960:CDS 60.0%
GGAAAGGGCAGTCCTCTGCT+AGG - chr2.2:59860421-59860440 MS.gene01960:CDS 60.0%
GGCTCAATCGCCCAATCTGG+TGG + chr2.2:59859324-59859343 None:intergenic 60.0%
GGGAAATTGATCCCCGTGGG+TGG + chr2.2:59860066-59860085 None:intergenic 60.0%
TGAACGAGCGCCAGCAGCTA+AGG - chr2.2:59860602-59860621 MS.gene01960:CDS 60.0%
TTGATCCCCGTGGGTGGTGA+AGG + chr2.2:59860060-59860079 None:intergenic 60.0%
TTGGTGGAGGGCAGTCGATG+TGG - chr2.2:59859295-59859314 MS.gene01960:CDS 60.0%
! CTGATGGGTCAGGAGGTGGT+GGG + chr2.2:59860086-59860105 None:intergenic 60.0%
! GAAAGGTGTGGGAGTGGAGG+CGG - chr2.2:59859236-59859255 MS.gene01960:CDS 60.0%
! TCTGATGGGTCAGGAGGTGG+TGG + chr2.2:59860087-59860106 None:intergenic 60.0%
AGGGCAGTCCTCTGCTAGGG+AGG - chr2.2:59860425-59860444 MS.gene01960:CDS 65.0%
AGTGGAGGCGGCTCCGTTGT+TGG - chr2.2:59859248-59859267 MS.gene01960:CDS 65.0%
CCCTCGTCCTTCACCACCCA+CGG - chr2.2:59860050-59860069 MS.gene01960:intron 65.0%
CCGTGGGTGGTGAAGGACGA+GGG + chr2.2:59860053-59860072 None:intergenic 65.0%
CCTCGTCCTTCACCACCCAC+GGG - chr2.2:59860051-59860070 MS.gene01960:intron 65.0%
CGCGGCTCAATCGCCCAATC+TGG + chr2.2:59859327-59859346 None:intergenic 65.0%
CTCGTCCTTCACCACCCACG+GGG - chr2.2:59860052-59860071 MS.gene01960:intron 65.0%
CTCTCGCCAACATGCTGCGG+TGG - chr2.2:59859476-59859495 MS.gene01960:CDS 65.0%
GTGGAGGCGGCTCCGTTGTT+GGG - chr2.2:59859249-59859268 MS.gene01960:CDS 65.0%
TGGAGGCGGCTCCGTTGTTG+GGG - chr2.2:59859250-59859269 MS.gene01960:CDS 65.0%
TTGGGCGATTGAGCCGCGAC+CGG - chr2.2:59859329-59859348 MS.gene01960:CDS 65.0%
CCCGTGGGTGGTGAAGGACG+AGG + chr2.2:59860054-59860073 None:intergenic 70.0%
TGGGCGATTGAGCCGCGACC+GGG - chr2.2:59859330-59859349 MS.gene01960:CDS 70.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 59859039 59860701 59859039 ID=MS.gene01960
chr2.2 mRNA 59859039 59860701 59859039 ID=MS.gene01960.t1;Parent=MS.gene01960
chr2.2 exon 59860223 59860701 59860223 ID=MS.gene01960.t1.exon1;Parent=MS.gene01960.t1
chr2.2 CDS 59860223 59860701 59860223 ID=cds.MS.gene01960.t1;Parent=MS.gene01960.t1
chr2.2 exon 59859039 59859846 59859039 ID=MS.gene01960.t1.exon2;Parent=MS.gene01960.t1
chr2.2 CDS 59859039 59859846 59859039 ID=cds.MS.gene01960.t1;Parent=MS.gene01960.t1
Gene Sequence

>MS.gene01960

ATGTATGGAAGAACATCTGGTCTTGATAGGTTTAAGAAAGCCGAGAGTTTGGAACCGTTCTCTGTTAATGGTAATTCGTCTTCGAGAAATGTTGCTCAGTCTTCTAGTAAGGTAGTTGGTCATTCTTCGGCTTGGCTGCCTCCACAGTCGAAGAATTCTGTACATCAATCACAGAATCAACATCAACATGGTTCTCAGAAAGGTGTGGGAGTGGAGGCGGCTCCGTTGTTGGGGCAGAATCAACAGGCAACTCAGATTGGTGGAGGGCAGTCGATGTGGCAGCCACCAGATTGGGCGATTGAGCCGCGACCGGGTGTTTTTTACTTGGAAGTTATGAAGGATGGTCAGGTGCTAGATAGGATTAATTTGGATAGACGAAGAAATATTTTTGGGAGGCAAATCCAGACTTGTGATTTTGTGCTTGATCATCAATCTGTCTCTCGCCAACATGCTGCGGTGGTTCCTCACAAGAACGGAAGGTTAGAACAGATCTCTCTCTCCTTTGATGTTACTGGAATGGATGTGATGTTTCTCTATATAAATATGTCATTTTCTTTCCGAATTGCATATTCTAAATAATTTGTCTCAAAAGTATTCAAAAACTAAAGCTTCTTTTCCAATAGCTTGCATCTATTACCTGTATCAAATGACACCATATTACCTTATCAAACGAAAGCATTCAAAGATACTAATTCGTGTTAATCAGTCGTTCATAAAAATGAATTACAAATGACTTAGGAACCACTTCAGCACTTTTAGCTTTTAGATATTAAACTAAAAATATGTCTTATATTATTTAGGAATGATGATTCCCTTGCAACTTATAGTTGTAATATTATTTTTACTTTATTTCAGTGTATATGTAATTGATTTGGGATCTGCACATGGTACCTTTGTTGCAAATGAGCGTTTGACCAAGGATTCACCTGTTGAGTTTGAAGTGGGACAATCATTGCGGTTTGCTGCATCCACGAGATTATACATATTAAGAAAGAATGATGCCGCTCTTTTCCCTCGTCCTTCACCACCCACGGGGATCAATTTCCCACCACCTCCTGACCCATCAGATGAAGAGGCTGTTGTTACTTATAATACGCTGCTGAATCGTTATGGCATCAACAAGTCTGATCTCGTGTCCAAATCCACTGAGCTTGGTAGTGCAACAAATGGCAAAAACAAGGATTGCCAATCAGAAAGAGCTGCTAAGAGAATTAAGAAGATGAGAGTTTCATTCAGGGATCAGGTTGGGGGAGAGTTAGTTGAGGTTGTTGGAATTTCAGATGGTGTAGATGTAGAAACAGAACCTGGTCCAGTTGGTGTTAAGGAAGGAAGTCTAGTTGGTAAATATGAATCTCTTGTACAAACTACTGTAATTCCAAAGGGGAAAGGGCAGTCCTCTGCTAGGGAGGTAGATTCATCCCAAAAAGGTGTGACTGATAAACTACAAGAAGTCCTAAACAAGATCAAAAACCCCGCTAAAACTGGGATTTACGATGACCTATATGGAGAATCATTATCTGTCAAAGTTGGATCAGCCTGGGCATACTCACCTGTCAGTAGTGGTGAACGAGCGCCAGCAGCTAAGGAGAATGGAGAAAGTGGAAAATCAGACAGTAATCCAAGCATTGCTGATTACGAGGATGATGATGATTTGTTTGGGTGA
Protein sequence

>MS.gene01960.t1

MYGRTSGLDRFKKAESLEPFSVNGNSSSRNVAQSSSKVVGHSSAWLPPQSKNSVHQSQNQHQHGSQKGVGVEAAPLLGQNQQATQIGGGQSMWQPPDWAIEPRPGVFYLEVMKDGQVLDRINLDRRRNIFGRQIQTCDFVLDHQSVSRQHAAVVPHKNGSVYVIDLGSAHGTFVANERLTKDSPVEFEVGQSLRFAASTRLYILRKNDAALFPRPSPPTGINFPPPPDPSDEEAVVTYNTLLNRYGINKSDLVSKSTELGSATNGKNKDCQSERAAKRIKKMRVSFRDQVGGELVEVVGISDGVDVETEPGPVGVKEGSLVGKYESLVQTTVIPKGKGQSSAREVDSSQKGVTDKLQEVLNKIKNPAKTGIYDDLYGESLSVKVGSAWAYSPVSSGERAPAAKENGESGKSDSNPSIADYEDDDDLFG