Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene021464.t1 | XP_003588527.1 | 99.1 | 218 | 2 | 0 | 3 | 220 | 36 | 253 | 4.10E-112 | 414.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene021464.t1 | G7I4B0 | 99.1 | 218 | 2 | 0 | 3 | 220 | 36 | 253 | 3.0e-112 | 414.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene021464 | MS.gene06720 | PPI |
| MS.gene56037 | MS.gene021464 | PPI |
| MS.gene021464 | MS.gene31626 | PPI |
| MS.gene021464 | MS.gene013601 | PPI |
| MS.gene021464 | MS.gene56037 | PPI |
| MS.gene021464 | MS.gene059145 | PPI |
| MS.gene021464 | MS.gene06938 | PPI |
| MS.gene021464 | MS.gene021465 | PPI |
| MS.gene021464 | MS.gene56038 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene021464.t1 | MTR_1g008220 | 99.083 | 218 | 2 | 0 | 3 | 220 | 36 | 253 | 1.37e-159 | 441 |
| MS.gene021464.t1 | MTR_1g008220 | 99.083 | 218 | 2 | 0 | 3 | 220 | 36 | 253 | 2.32e-159 | 442 |
| MS.gene021464.t1 | MTR_3g091340 | 72.973 | 222 | 52 | 4 | 1 | 220 | 7 | 222 | 1.38e-101 | 295 |
| MS.gene021464.t1 | MTR_3g091340 | 72.727 | 220 | 52 | 4 | 3 | 220 | 2 | 215 | 5.11e-100 | 290 |
| MS.gene021464.t1 | MTR_3g091340 | 72.727 | 220 | 52 | 4 | 3 | 220 | 38 | 251 | 3.29e-99 | 290 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene021464.t1 | AT5G53420 | 49.550 | 222 | 93 | 8 | 1 | 220 | 35 | 239 | 2.76e-60 | 190 |
| MS.gene021464.t1 | AT4G27900 | 46.957 | 230 | 90 | 6 | 4 | 220 | 25 | 235 | 1.19e-56 | 181 |
| MS.gene021464.t1 | AT4G27900 | 46.957 | 230 | 90 | 6 | 4 | 220 | 25 | 235 | 1.19e-56 | 181 |
| MS.gene021464.t1 | AT5G53420 | 51.412 | 177 | 67 | 8 | 46 | 220 | 1 | 160 | 3.64e-46 | 151 |
| MS.gene021464.t1 | AT5G53420 | 51.412 | 177 | 67 | 8 | 46 | 220 | 1 | 160 | 3.64e-46 | 151 |
| MS.gene021464.t1 | AT5G53420 | 51.412 | 177 | 67 | 8 | 46 | 220 | 1 | 160 | 3.64e-46 | 151 |
| MS.gene021464.t1 | AT5G53420 | 45.763 | 177 | 82 | 6 | 1 | 175 | 35 | 199 | 1.82e-40 | 137 |
Find 48 sgRNAs with CRISPR-Local
Find 77 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTCTTCTTATTCCTATATC+TGG | 0.246824 | 1.4:-43249017 | None:intergenic |
| TTCAGGAAATTTGAGTTCAA+TGG | 0.350069 | 1.4:+43248374 | MS.gene021464:CDS |
| GATAATAGTACATCAGGAAT+TGG | 0.361584 | 1.4:-43248339 | None:intergenic |
| CCTGCTTTCCTTGATTTCCC+TGG | 0.370726 | 1.4:+43248423 | MS.gene021464:CDS |
| AAGAAGACAAGGAGAAACTT+TGG | 0.387483 | 1.4:+43249031 | MS.gene021464:CDS |
| AGGAAAGCAGGCTTCATTGC+AGG | 0.402149 | 1.4:-43248411 | None:intergenic |
| ACCGCGTTAAAATCAATTCC+AGG | 0.422111 | 1.4:-43248441 | None:intergenic |
| GAAATTTGAGTTCAATGGAT+TGG | 0.431971 | 1.4:+43248379 | MS.gene021464:CDS |
| TTATCAAAGAGTGTCAGTTC+AGG | 0.440427 | 1.4:+43248357 | MS.gene021464:CDS |
| GAGCAGGAATCTTGATTTCA+AGG | 0.444374 | 1.4:-43248281 | None:intergenic |
| CTCTTTGATAATAGTACATC+AGG | 0.447091 | 1.4:-43248345 | None:intergenic |
| AGAAGACAAGGAGAAACTTT+GGG | 0.461172 | 1.4:+43249032 | MS.gene021464:CDS |
| GTGCAGTTGCTGAGAAGAAA+AGG | 0.470162 | 1.4:-43248968 | None:intergenic |
| AATCAATTCCAGGGAAATCA+AGG | 0.470610 | 1.4:-43248431 | None:intergenic |
| TGGGGAAGACATGTCGTCGC+AGG | 0.471749 | 1.4:+43248137 | MS.gene021464:CDS |
| ATTTAGTGAAGGAGATATAA+AGG | 0.474162 | 1.4:+43248482 | MS.gene021464:CDS |
| CGGTTCTTCAATAATCGTCT+CGG | 0.477444 | 1.4:-43248067 | None:intergenic |
| CGAGACGATTATTGAAGAAC+CGG | 0.482715 | 1.4:+43248068 | MS.gene021464:CDS |
| TGGCTGCTTGCATTTGATCA+AGG | 0.489809 | 1.4:-43248098 | None:intergenic |
| GTCGCAGGGTCTAAAATCTT+CGG | 0.499847 | 1.4:+43248152 | MS.gene021464:CDS |
| TTGTTTGTATAACAGACTCT+TGG | 0.509295 | 1.4:+43248905 | MS.gene021464:intron |
| AGAATTTGATTTATTTGTAG+AGG | 0.514069 | 1.4:+43248026 | MS.gene021464:CDS |
| TTCTCGTCAATGTTCAGAGC+AGG | 0.527138 | 1.4:-43248297 | None:intergenic |
| ATTTGATCAAGGTCCATCTC+CGG | 0.536269 | 1.4:-43248087 | None:intergenic |
| CCAGGGAAATCAAGGAAAGC+AGG | 0.538494 | 1.4:-43248423 | None:intergenic |
| GAATTTGATTTATTTGTAGA+GGG | 0.539422 | 1.4:+43248027 | MS.gene021464:CDS |
| AGTTCAATGGATTGGATGCA+TGG | 0.542974 | 1.4:+43248387 | MS.gene021464:CDS |
| ATTTCAAGGATTTCAGAAAG+AGG | 0.543254 | 1.4:-43248267 | None:intergenic |
| GCAGCCATGTCAATGATGTC+TGG | 0.545025 | 1.4:+43248114 | MS.gene021464:CDS |
| GATTATTGAAGAACCGGAGA+TGG | 0.549985 | 1.4:+43248074 | MS.gene021464:CDS |
| GGTTCTTCAATAATCGTCTC+GGG | 0.551243 | 1.4:-43248066 | None:intergenic |
| CCGCGTTAAAATCAATTCCA+GGG | 0.559154 | 1.4:-43248440 | None:intergenic |
| AAGGCCTCTCTAGAGTGGAT+TGG | 0.563933 | 1.4:-43248949 | None:intergenic |
| AAACTTTGGGAGGAAAATCA+AGG | 0.564099 | 1.4:+43249045 | MS.gene021464:CDS |
| GGGGAAGACATGTCGTCGCA+GGG | 0.576526 | 1.4:+43248138 | MS.gene021464:CDS |
| AAGAAAAGGCCTCTCTAGAG+TGG | 0.578670 | 1.4:-43248954 | None:intergenic |
| CCATGTCAATGATGTCTGGT+GGG | 0.605089 | 1.4:+43248118 | MS.gene021464:CDS |
| GTATAACAGACTCTTGGCAA+TGG | 0.606169 | 1.4:+43248911 | MS.gene021464:intron |
| GGCAATGGTAACATGAGTGT+TGG | 0.622717 | 1.4:+43248926 | MS.gene021464:CDS |
| CCCACCAGACATCATTGACA+TGG | 0.626711 | 1.4:-43248118 | None:intergenic |
| GCCATGTCAATGATGTCTGG+TGG | 0.631276 | 1.4:+43248117 | MS.gene021464:CDS |
| GATATAGGAATAAGAAGACA+AGG | 0.632233 | 1.4:+43249020 | MS.gene021464:CDS |
| ATGCGAAGAGCATTTAGTGA+AGG | 0.636958 | 1.4:+43248471 | MS.gene021464:CDS |
| AGAAAGAGGTGATTCTATAG+CGG | 0.645696 | 1.4:-43248253 | None:intergenic |
| TTGGCCAATCCACTCTAGAG+AGG | 0.651640 | 1.4:+43248945 | MS.gene021464:CDS |
| ACATCGCATATGATTGAACA+AGG | 0.660417 | 1.4:-43248002 | None:intergenic |
| CATGTCAATGATGTCTGGTG+GGG | 0.675165 | 1.4:+43248119 | MS.gene021464:CDS |
| AGACAAGGAGAAACTTTGGG+AGG | 0.688525 | 1.4:+43249035 | MS.gene021464:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATAAATTAAATAACATCTA+TGG | + | chr1.4:43248543-43248562 | MS.gene021464:intron | 10.0% |
| !! | TTAAACATTATATGTTGTAT+TGG | + | chr1.4:43248574-43248593 | MS.gene021464:intron | 15.0% |
| !! | ACATATAATGTTTAACAAAG+TGG | - | chr1.4:43248569-43248588 | None:intergenic | 20.0% |
| !! | TATAAATTAAGTAACATCCA+TGG | - | chr1.4:43248822-43248841 | None:intergenic | 20.0% |
| !!! | AGAATTTGATTTATTTGTAG+AGG | + | chr1.4:43248026-43248045 | MS.gene021464:CDS | 20.0% |
| !!! | GAATTTGATTTATTTGTAGA+GGG | + | chr1.4:43248027-43248046 | MS.gene021464:CDS | 20.0% |
| !!! | TTAAATTTTTATATTGGACC+TGG | - | chr1.4:43248697-43248716 | None:intergenic | 20.0% |
| ! | AGATATAAAGGTCAACTTAA+AGG | + | chr1.4:43248494-43248513 | MS.gene021464:intron | 25.0% |
| ! | ATTTAGTGAAGGAGATATAA+AGG | + | chr1.4:43248482-43248501 | MS.gene021464:CDS | 25.0% |
| ! | CAAAAAAGATCTCTTAGAAA+AGG | + | chr1.4:43248230-43248249 | MS.gene021464:CDS | 25.0% |
| ! | TTTGCCATATCAAAATGATT+TGG | + | chr1.4:43248869-43248888 | MS.gene021464:intron | 25.0% |
| !! | ATATAATGTCTAAGACAAAG+AGG | + | chr1.4:43248796-43248815 | MS.gene021464:intron | 25.0% |
| !! | TCTTACAAAACTAGTTTGTA+AGG | - | chr1.4:43248669-43248688 | None:intergenic | 25.0% |
| !!! | ATCATTTTGATATGGCAAAT+AGG | - | chr1.4:43248868-43248887 | None:intergenic | 25.0% |
| !!! | TACACCAAATCATTTTGATA+TGG | - | chr1.4:43248876-43248895 | None:intergenic | 25.0% |
| AAGAAAAACTCTCCAGATAT+AGG | + | chr1.4:43249005-43249024 | MS.gene021464:CDS | 30.0% | |
| ATTTCAAGGATTTCAGAAAG+AGG | - | chr1.4:43248270-43248289 | None:intergenic | 30.0% | |
| GATAATAGTACATCAGGAAT+TGG | - | chr1.4:43248342-43248361 | None:intergenic | 30.0% | |
| GATATAGGAATAAGAAGACA+AGG | + | chr1.4:43249020-43249039 | MS.gene021464:CDS | 30.0% | |
| TATGTTGATGTATCTAGACT+CGG | + | chr1.4:43248717-43248736 | MS.gene021464:intron | 30.0% | |
| TGTCTTCTTATTCCTATATC+TGG | - | chr1.4:43249020-43249039 | None:intergenic | 30.0% | |
| TTGTTTGTATAACAGACTCT+TGG | + | chr1.4:43248905-43248924 | MS.gene021464:intron | 30.0% | |
| ! | AAAACTAGTTTGTAAGGTGA+GGG | - | chr1.4:43248663-43248682 | None:intergenic | 30.0% |
| ! | CTCTTTGATAATAGTACATC+AGG | - | chr1.4:43248348-43248367 | None:intergenic | 30.0% |
| ! | GAAATTTGAGTTCAATGGAT+TGG | + | chr1.4:43248379-43248398 | MS.gene021464:CDS | 30.0% |
| ! | TTCAGGAAATTTGAGTTCAA+TGG | + | chr1.4:43248374-43248393 | MS.gene021464:CDS | 30.0% |
| !! | ATTGATTTTAACGCGGTTTA+TGG | + | chr1.4:43248447-43248466 | MS.gene021464:CDS | 30.0% |
| AAGAAGACAAGGAGAAACTT+TGG | + | chr1.4:43249031-43249050 | MS.gene021464:CDS | 35.0% | |
| AATCAATTCCAGGGAAATCA+AGG | - | chr1.4:43248434-43248453 | None:intergenic | 35.0% | |
| ACATCGCATATGATTGAACA+AGG | - | chr1.4:43248005-43248024 | None:intergenic | 35.0% | |
| AGAAAGAGGTGATTCTATAG+CGG | - | chr1.4:43248256-43248275 | None:intergenic | 35.0% | |
| AGAAGACAAGGAGAAACTTT+GGG | + | chr1.4:43249032-43249051 | MS.gene021464:CDS | 35.0% | |
| ATGTTGATGTATCTAGACTC+GGG | + | chr1.4:43248718-43248737 | MS.gene021464:intron | 35.0% | |
| ! | AAACTAGTTTGTAAGGTGAG+GGG | - | chr1.4:43248662-43248681 | None:intergenic | 35.0% |
| ! | CAAAACTAGTTTGTAAGGTG+AGG | - | chr1.4:43248664-43248683 | None:intergenic | 35.0% |
| !! | TTATCAAAGAGTGTCAGTTC+AGG | + | chr1.4:43248357-43248376 | MS.gene021464:CDS | 35.0% |
| !!! | TAGTTTTGTAAGAATGAGCC+AGG | + | chr1.4:43248676-43248695 | MS.gene021464:intron | 35.0% |
| ACCGCGTTAAAATCAATTCC+AGG | - | chr1.4:43248444-43248463 | None:intergenic | 40.0% | |
| ATTTGATCAAGGTCCATCTC+CGG | - | chr1.4:43248090-43248109 | None:intergenic | 40.0% | |
| CCGCGTTAAAATCAATTCCA+GGG | - | chr1.4:43248443-43248462 | None:intergenic | 40.0% | |
| CGAGACGATTATTGAAGAAC+CGG | + | chr1.4:43248068-43248087 | MS.gene021464:CDS | 40.0% | |
| CGGTTCTTCAATAATCGTCT+CGG | - | chr1.4:43248070-43248089 | None:intergenic | 40.0% | |
| CGTTGAGAAGAGATAAGACT+TGG | + | chr1.4:43248614-43248633 | MS.gene021464:intron | 40.0% | |
| GAGCAGGAATCTTGATTTCA+AGG | - | chr1.4:43248284-43248303 | None:intergenic | 40.0% | |
| GATTATTGAAGAACCGGAGA+TGG | + | chr1.4:43248074-43248093 | MS.gene021464:CDS | 40.0% | |
| GGTTCTTCAATAATCGTCTC+GGG | - | chr1.4:43248069-43248088 | None:intergenic | 40.0% | |
| GTATAACAGACTCTTGGCAA+TGG | + | chr1.4:43248911-43248930 | MS.gene021464:intron | 40.0% | |
| GTCTTATCTCTTCTCAACGT+GGG | - | chr1.4:43248613-43248632 | None:intergenic | 40.0% | |
| ! | AGTCTTATCTCTTCTCAACG+TGG | - | chr1.4:43248614-43248633 | None:intergenic | 40.0% |
| !! | AAGCGAACAACTTTTGAGTG+AGG | + | chr1.4:43248194-43248213 | MS.gene021464:CDS | 40.0% |
| !! | AGTTCAATGGATTGGATGCA+TGG | + | chr1.4:43248387-43248406 | MS.gene021464:CDS | 40.0% |
| !! | ATGCGAAGAGCATTTAGTGA+AGG | + | chr1.4:43248471-43248490 | MS.gene021464:CDS | 40.0% |
| !! | CCCTGGAATTGATTTTAACG+CGG | + | chr1.4:43248440-43248459 | MS.gene021464:CDS | 40.0% |
| AGACAAGGAGAAACTTTGGG+AGG | + | chr1.4:43249035-43249054 | MS.gene021464:CDS | 45.0% | |
| CATGTCAATGATGTCTGGTG+GGG | + | chr1.4:43248119-43248138 | MS.gene021464:CDS | 45.0% | |
| CCATGTCAATGATGTCTGGT+GGG | + | chr1.4:43248118-43248137 | MS.gene021464:CDS | 45.0% | |
| GGCAATGGTAACATGAGTGT+TGG | + | chr1.4:43248926-43248945 | MS.gene021464:CDS | 45.0% | |
| GTGCAGTTGCTGAGAAGAAA+AGG | - | chr1.4:43248971-43248990 | None:intergenic | 45.0% | |
| TGGCTGCTTGCATTTGATCA+AGG | - | chr1.4:43248101-43248120 | None:intergenic | 45.0% | |
| TTCTCGTCAATGTTCAGAGC+AGG | - | chr1.4:43248300-43248319 | None:intergenic | 45.0% | |
| ! | AAGAAAAGGCCTCTCTAGAG+TGG | - | chr1.4:43248957-43248976 | None:intergenic | 45.0% |
| ! | GTCGCAGGGTCTAAAATCTT+CGG | + | chr1.4:43248152-43248171 | MS.gene021464:CDS | 45.0% |
| ! | TGTCTAAGACAAAGAGGCCA+TGG | + | chr1.4:43248802-43248821 | MS.gene021464:intron | 45.0% |
| !!! | AACATATTAAATTTTTATAT+TGG | - | chr1.4:43248703-43248722 | None:intergenic | 5.0% |
| AGGAAAGCAGGCTTCATTGC+AGG | - | chr1.4:43248414-43248433 | None:intergenic | 50.0% | |
| CCAGGGAAATCAAGGAAAGC+AGG | - | chr1.4:43248426-43248445 | None:intergenic | 50.0% | |
| CCCACCAGACATCATTGACA+TGG | - | chr1.4:43248121-43248140 | None:intergenic | 50.0% | |
| CCTGCTTTCCTTGATTTCCC+TGG | + | chr1.4:43248423-43248442 | MS.gene021464:CDS | 50.0% | |
| GCAGCCATGTCAATGATGTC+TGG | + | chr1.4:43248114-43248133 | MS.gene021464:CDS | 50.0% | |
| GCCATGTCAATGATGTCTGG+TGG | + | chr1.4:43248117-43248136 | MS.gene021464:CDS | 50.0% | |
| ! | TTGGCCAATCCACTCTAGAG+AGG | + | chr1.4:43248945-43248964 | MS.gene021464:CDS | 50.0% |
| !! | AAGGCCTCTCTAGAGTGGAT+TGG | - | chr1.4:43248952-43248971 | None:intergenic | 50.0% |
| GTCCTCTTTAGCGCTGCACA+CGG | + | chr1.4:43248740-43248759 | MS.gene021464:intron | 55.0% | |
| TTAGCGCTGCACACGGTGAA+CGG | + | chr1.4:43248747-43248766 | MS.gene021464:intron | 55.0% | |
| CACCGTGTGCAGCGCTAAAG+AGG | - | chr1.4:43248745-43248764 | None:intergenic | 60.0% | |
| GGGGAAGACATGTCGTCGCA+GGG | + | chr1.4:43248138-43248157 | MS.gene021464:CDS | 60.0% | |
| TGGGGAAGACATGTCGTCGC+AGG | + | chr1.4:43248137-43248156 | MS.gene021464:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 43247991 | 43249066 | 43247991 | ID=MS.gene021464 |
| chr1.4 | mRNA | 43247991 | 43249066 | 43247991 | ID=MS.gene021464.t1;Parent=MS.gene021464 |
| chr1.4 | exon | 43247991 | 43248503 | 43247991 | ID=MS.gene021464.t1.exon1;Parent=MS.gene021464.t1 |
| chr1.4 | CDS | 43247991 | 43248503 | 43247991 | ID=cds.MS.gene021464.t1;Parent=MS.gene021464.t1 |
| chr1.4 | exon | 43248920 | 43249066 | 43248920 | ID=MS.gene021464.t1.exon2;Parent=MS.gene021464.t1 |
| chr1.4 | CDS | 43248920 | 43249066 | 43248920 | ID=cds.MS.gene021464.t1;Parent=MS.gene021464.t1 |
| Gene Sequence |
| Protein sequence |
