AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene022779

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene022779.t1 MTR_8g011270 99.607 763 3 0 1 763 1 763 0.0 1573
MS.gene022779.t1 MTR_3g084740 64.276 767 260 5 7 763 8 770 0.0 1032
MS.gene022779.t1 MTR_3g084740 62.700 689 244 4 7 686 8 692 0.0 907
MS.gene022779.t1 MTR_1g105605 43.282 774 395 11 4 763 2 745 0.0 617
MS.gene022779.t1 MTR_6g452530 92.754 138 9 1 540 677 7 143 5.72e-82 259
MS.gene022779.t1 MTR_6g452520 92.754 138 9 1 540 677 7 143 1.60e-81 258
MS.gene022779.t1 MTR_6g452510 92.754 138 9 1 540 677 7 143 1.60e-81 258
MS.gene022779.t1 MTR_7g110640 21.935 734 458 23 36 738 162 811 1.61e-30 129
MS.gene022779.t1 MTR_4g052930 21.818 715 442 29 6 666 12 663 1.59e-11 68.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene022779.t1 AT2G21630 70.380 763 219 4 4 763 3 761 0.0 1099
MS.gene022779.t1 AT3G23660 62.924 766 274 4 3 763 4 764 0.0 1014
MS.gene022779.t1 AT4G14160 62.254 763 283 4 4 763 11 771 0.0 1013
MS.gene022779.t1 AT4G14160 62.173 764 283 5 4 763 11 772 0.0 1008
MS.gene022779.t1 AT3G23660 60.859 792 274 5 3 763 4 790 0.0 1001
MS.gene022779.t1 AT1G05520 60.875 777 284 4 6 763 7 782 0.0 996
MS.gene022779.t1 AT4G14160 59.022 593 239 3 4 594 11 601 0.0 736
MS.gene022779.t1 AT5G43670 42.875 807 399 9 4 763 2 793 0.0 655
MS.gene022779.t1 AT4G01810 22.315 717 447 24 55 738 201 840 1.27e-33 139
MS.gene022779.t1 AT4G01810 22.315 717 447 24 55 738 201 840 1.27e-33 139
MS.gene022779.t1 AT4G01810 22.315 717 447 24 55 738 201 840 1.27e-33 139
MS.gene022779.t1 AT2G27460 20.994 724 437 24 6 666 12 663 2.37e-13 74.3

Find 200 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATAGAATTTCACACATTTA+TGG 0.172759 8.1:+85340243 None:intergenic
ACCTCTAAACACATAAGTTT+TGG 0.211068 8.1:+85342055 None:intergenic
TATGATTCCGTTAACGATTC+TGG 0.213494 8.1:-85342257 MS.gene022779:CDS
TTTGTTCATGTTCATGAGTT+AGG 0.216501 8.1:-85342092 MS.gene022779:CDS
GGCTGACAGGGTTCTGCTTT+TGG 0.228877 8.1:-85336013 MS.gene022779:CDS
TTATGCTCAGATGTTGTTAT+TGG 0.238761 8.1:-85338049 MS.gene022779:CDS
TTATGTGTTTAGAGGTAATA+AGG 0.254744 8.1:-85342048 MS.gene022779:CDS
CCCAAAACTTATGTGTTTAG+AGG 0.255169 8.1:-85342056 MS.gene022779:CDS
TTGTTGGGTGCTTGTGTTCC+TGG 0.255191 8.1:-85341254 MS.gene022779:CDS
ATTTCATATTCATTCCATTC+TGG 0.275890 8.1:-85336072 MS.gene022779:CDS
CTTGTTGGATTAATTACATT+TGG 0.277025 8.1:-85342119 MS.gene022779:CDS
ACAGGCACATTACCGAATTA+AGG 0.281286 8.1:+85341877 None:intergenic
CGGCAACGGAGAGGAGAGTA+TGG 0.281571 8.1:+85342443 None:intergenic
TGACTATAGCAGCTGCTTCT+TGG 0.296847 8.1:+85337097 None:intergenic
GCAAGAATAATGGCGTTTAT+TGG 0.297801 8.1:-85341224 MS.gene022779:CDS
CCAATCTGGTGATTATGATC+TGG 0.297814 8.1:-85339449 MS.gene022779:CDS
TTACAACTCTTTCACGTAGA+TGG 0.300540 8.1:-85337750 MS.gene022779:CDS
ATCAAAGTCCAAGGGATTAT+TGG 0.303965 8.1:-85338204 MS.gene022779:CDS
CAGTGGACAAATGAGATTTC+GGG 0.307184 8.1:-85337773 MS.gene022779:CDS
GAGATTGAAGCGTAATGATT+AGG 0.314527 8.1:+85342329 None:intergenic
CCGGCGGTTGGTGTTGTTGC+CGG 0.317183 8.1:-85341969 MS.gene022779:CDS
ATAAATTTGTGTTCACGTTT+CGG 0.324061 8.1:-85336404 MS.gene022779:CDS
TGGAGGTGACATTATCTTTA+CGG 0.329392 8.1:-85334937 MS.gene022779:CDS
CGCAATGCTCAGCGCCGTGC+TGG 0.339381 8.1:+85341283 None:intergenic
TTGATTTCGCCGCCAAGATC+TGG 0.341471 8.1:-85342385 MS.gene022779:CDS
TTTCATACTAAGTCAAGAAT+TGG 0.343406 8.1:+85337897 None:intergenic
GGAGCAGGTCCTTCTGTTGC+TGG 0.349801 8.1:+85341197 None:intergenic
CATGTTCATGAGTTAGGGTT+TGG 0.352202 8.1:-85342086 MS.gene022779:CDS
ATGTGAATTCGCCCTTAATT+CGG 0.357344 8.1:-85341889 MS.gene022779:intron
CCAGAGAATACCTGATGTTC+TGG 0.361604 8.1:+85335916 None:intergenic
ACGCCAAGTGTCTTTCAAAA+TGG 0.363384 8.1:-85337070 MS.gene022779:CDS
ACAGTGGACAAATGAGATTT+CGG 0.364007 8.1:-85337774 MS.gene022779:CDS
CAATCTGGTGATTATGATCT+GGG 0.367381 8.1:-85339448 MS.gene022779:CDS
TTGCCGGATAATTCGCTTGT+TGG 0.367608 8.1:-85342134 MS.gene022779:CDS
TTCAAAGCAGCTAGTGCATC+AGG 0.371866 8.1:-85340213 MS.gene022779:CDS
CTAAAGTAAGCAGTCTCGTC+TGG 0.373492 8.1:+85336153 None:intergenic
AGGTAATAAGGATTTGTCTA+AGG 0.373932 8.1:-85342036 MS.gene022779:CDS
CTCGCCTCCCGTTGATCACC+TGG 0.377392 8.1:+85341311 None:intergenic
TGGTTCTGCCGCAAGAATAA+TGG 0.377699 8.1:-85341234 MS.gene022779:CDS
CCCTTGTGCTTCTCTTGAAA+AGG 0.377918 8.1:-85338181 MS.gene022779:intron
TTGTTCATGTTCATGAGTTA+GGG 0.378111 8.1:-85342091 MS.gene022779:CDS
CTTTGCTTGCGCTCTTGATC+AGG 0.378710 8.1:-85340174 MS.gene022779:intron
ATAACAACATCTGAGCATAA+TGG 0.380339 8.1:+85338052 None:intergenic
AATTGGTTCAGAAAGTTGTT+TGG 0.382842 8.1:+85340307 None:intergenic
ACGACGTTCTCAGTTTGTTC+AGG 0.386590 8.1:-85336303 MS.gene022779:intron
TTGTTGGATTAATTACATTT+GGG 0.387705 8.1:-85342118 MS.gene022779:CDS
AAGCTTACCTGTATGCTTCC+TGG 0.388950 8.1:+85337718 None:intergenic
AGCATTGCGGCTAGTTTGTT+GGG 0.396881 8.1:-85341269 MS.gene022779:CDS
GATATCCAGCCTTCCGCCAC+TGG 0.397925 8.1:+85335947 None:intergenic
ATTTGAGATAAACTGCTCTA+AGG 0.400423 8.1:-85338229 MS.gene022779:CDS
TCGCAGCCATTGCGGCTGAC+AGG 0.404401 8.1:-85336026 MS.gene022779:CDS
GTGTTTGATTGGAGTGTAGA+TGG 0.404761 8.1:+85342487 None:intergenic
GAGCATTGCGGCTAGTTTGT+TGG 0.405888 8.1:-85341270 MS.gene022779:CDS
TGGCATTCGAACGCCATCTT+GGG 0.408239 8.1:+85342568 None:intergenic
CTTTCACGTAGATGGGTTGC+TGG 0.408317 8.1:-85337742 MS.gene022779:CDS
TTGATCACCTGGCACCGGCC+AGG 0.409485 8.1:+85341322 None:intergenic
GTTGTACTTACTGAAAGTTT+TGG 0.416717 8.1:-85339508 MS.gene022779:CDS
AGGGATCCCTGGCCGGTGCC+AGG 0.416718 8.1:-85341329 MS.gene022779:CDS
ATCTTTATCCAGATCTTTGT+GGG 0.416722 8.1:+85340280 None:intergenic
TATGGGAGGAGTGGCATGGT+AGG 0.421443 8.1:+85342461 None:intergenic
ACAACACCAACCGCCGGCTT+CGG 0.425625 8.1:+85341975 None:intergenic
AGATCTTTGTGGGAGCGAAT+TGG 0.431679 8.1:+85340290 None:intergenic
TAACAACATCTGAGCATAAT+GGG 0.431894 8.1:+85338053 None:intergenic
CTTCATTTAGTTTGTCTCCC+AGG 0.432541 8.1:-85336358 MS.gene022779:CDS
TTGGTGTTGTTGCCGGTGCA+AGG 0.433855 8.1:-85341962 MS.gene022779:CDS
TTATTACTTACTTTGATGCT+AGG 0.434040 8.1:+85339425 None:intergenic
GCCGGTGCCAGGTGATCAAC+GGG 0.443699 8.1:-85341318 MS.gene022779:CDS
CAGGCACATTACCGAATTAA+GGG 0.445247 8.1:+85341878 None:intergenic
AATCTTTATCCAGATCTTTG+TGG 0.450820 8.1:+85340279 None:intergenic
AATTCGCTCCCACAAAGATC+TGG 0.451559 8.1:-85340288 MS.gene022779:CDS
GCGAATTCACATTCCGAAGC+TGG 0.452807 8.1:+85341900 None:intergenic
ATGGCATTCGAACGCCATCT+TGG 0.453601 8.1:+85342567 None:intergenic
GAGATTTCCTGTGCCTCGCC+TGG 0.454313 8.1:-85335139 MS.gene022779:CDS
ATTGCCCAGTGGCGGAAGGC+TGG 0.456740 8.1:-85335952 MS.gene022779:CDS
TGATCACCTGGCACCGGCCA+GGG 0.457622 8.1:+85341323 None:intergenic
AATCCAACAAGCGAATTATC+CGG 0.461492 8.1:+85342131 None:intergenic
CAGATGTTGTTATTGGTCAA+GGG 0.464231 8.1:-85338042 MS.gene022779:CDS
CGCAGCCATTGCGGCTGACA+GGG 0.466910 8.1:-85336025 MS.gene022779:CDS
TTTGTCTAAGGAGCAGTTGT+TGG 0.466913 8.1:-85342024 MS.gene022779:CDS
ACCTCCAGGGAGTGAAGCTT+CGG 0.474534 8.1:+85334953 None:intergenic
TCATAATGAACCAGAACATC+AGG 0.476605 8.1:-85335926 MS.gene022779:intron
ATGGGAGGAGTGGCATGGTA+GGG 0.477887 8.1:+85342462 None:intergenic
TACAACTCTTTCACGTAGAT+GGG 0.479551 8.1:-85337749 MS.gene022779:CDS
AAACTGCTGTTGAAAGGACT+GGG 0.482381 8.1:-85339537 MS.gene022779:CDS
GGAGACAAACTAAATGAAGA+TGG 0.483143 8.1:+85336362 None:intergenic
CGTAGATGGGTTGCTGGACC+AGG 0.484608 8.1:-85337736 MS.gene022779:CDS
CTAATCTCTGTAAATGATCA+AGG 0.485472 8.1:+85334892 None:intergenic
ATTATTCTTGCGGCAGAACC+AGG 0.489028 8.1:+85341236 None:intergenic
AGAGAAACGCTTACAGGAGC+AGG 0.490863 8.1:+85341182 None:intergenic
GGCCGGTGCCAGGTGATCAA+CGG 0.492835 8.1:-85341319 MS.gene022779:CDS
AAAACTGCTGTTGAAAGGAC+TGG 0.494421 8.1:-85339538 MS.gene022779:CDS
GCCTTGTCCAACCATCTGAT+AGG 0.495172 8.1:+85336431 None:intergenic
AAACGGACAGATCCAGATCT+TGG 0.497672 8.1:+85342373 None:intergenic
TGGAGTGTAGATGGCGGCGA+CGG 0.498547 8.1:+85342496 None:intergenic
AGAATAATGGCGTTTATTGG+TGG 0.499942 8.1:-85341221 MS.gene022779:CDS
AGCCTTCCGCCACTGGGCAA+TGG 0.504181 8.1:+85335954 None:intergenic
CGTAAGTGAAACATAAATTG+TGG 0.504287 8.1:+85336323 None:intergenic
ATGGTAGGGAAGTGTTTGAT+TGG 0.507799 8.1:+85342476 None:intergenic
AAATGTGTGAAATTCTATGA+TGG 0.509370 8.1:-85340239 MS.gene022779:CDS
AACACCGAAGCTTCACTCCC+TGG 0.509866 8.1:-85334957 MS.gene022779:CDS
GTGCCGGGGATTACGTTCCA+TGG 0.510397 8.1:+85342548 None:intergenic
TCAGATGTTGTTATTGGTCA+AGG 0.511647 8.1:-85338043 MS.gene022779:CDS
TTGAGCGGCAAGTGCGGCAA+CGG 0.512011 8.1:+85342429 None:intergenic
TTCACACATTTATGGTAATG+TGG 0.516644 8.1:+85340251 None:intergenic
ATGAGCTGCAGAGGGATCCC+TGG 0.517961 8.1:-85341340 MS.gene022779:CDS
TTAAGTGAATCCTTGAACAC+TGG 0.518208 8.1:+85339481 None:intergenic
TGTGTGATTGAAGAGGAAAT+TGG 0.519856 8.1:-85342194 MS.gene022779:CDS
ATTTGATCCTATCAGATGGT+TGG 0.520252 8.1:-85336438 MS.gene022779:CDS
CCAGATCATAATCACCAGAT+TGG 0.520401 8.1:+85339449 None:intergenic
GTGGATTGCATTGTAGCGTC+TGG 0.520785 8.1:+85337938 None:intergenic
TTGTGATCAGCATGGATCCC+AGG 0.521300 8.1:-85335112 MS.gene022779:intron
GTTGTTGCCGGTGCAAGGGA+TGG 0.522179 8.1:-85341957 MS.gene022779:CDS
GTGCGGTCCAGAATCGTTAA+CGG 0.524245 8.1:+85342250 None:intergenic
CACAAGGGCCAATAATCCCT+TGG 0.526051 8.1:+85338196 None:intergenic
GATTAGGAAAATGATTGCGC+TGG 0.526519 8.1:+85342345 None:intergenic
TTGTGGGAATATCGACAACC+TGG 0.527124 8.1:+85336340 None:intergenic
AGCTTAAAACTGCTGTTGAA+AGG 0.531045 8.1:-85339543 MS.gene022779:CDS
CGAAATCAACGATGCAGAAA+GGG 0.533942 8.1:+85342399 None:intergenic
GACGGGAACGACGACGTTTG+TGG 0.534643 8.1:+85342514 None:intergenic
TGCTGGACCAGGAAGCATAC+AGG 0.537072 8.1:-85337725 MS.gene022779:intron
GTAAGTGAAACATAAATTGT+GGG 0.537478 8.1:+85336324 None:intergenic
TGGTGTTGTTGCCGGTGCAA+GGG 0.538054 8.1:-85341961 MS.gene022779:CDS
GAGACAAACTAAATGAAGAT+GGG 0.541167 8.1:+85336363 None:intergenic
GGAGTGTAGATGGCGGCGAC+GGG 0.541727 8.1:+85342497 None:intergenic
ACATCGAGAAGAGCAGGCTC+AGG 0.542411 8.1:+85336048 None:intergenic
CAGAACCCTGTCAGCCGCAA+TGG 0.544160 8.1:+85336020 None:intergenic
GTAAAGATAATGTCACCTCC+AGG 0.544404 8.1:+85334939 None:intergenic
GCGAAATCAACGATGCAGAA+AGG 0.545654 8.1:+85342398 None:intergenic
AGTGTCTTTCAAAATGGAAA+CGG 0.548687 8.1:-85337064 MS.gene022779:CDS
TTTCGCTAAGAAACCGAAGC+CGG 0.550293 8.1:-85341988 MS.gene022779:CDS
GCTGCAGAGGGATCCCTGGC+CGG 0.550385 8.1:-85341336 MS.gene022779:CDS
GCTGCGACATCGAGAAGAGC+AGG 0.552124 8.1:+85336042 None:intergenic
AACCATTGCCCAGTGGCGGA+AGG 0.554058 8.1:-85335956 MS.gene022779:CDS
AGAGTATGGGAGGAGTGGCA+TGG 0.554935 8.1:+85342457 None:intergenic
TATTCAACGGTAGTGTATTG+AGG 0.557094 8.1:+85342278 None:intergenic
GAAGTAAAATTGATTGCTTG+TGG 0.557139 8.1:+85337919 None:intergenic
ATCGATCTCGAAACCCAAGA+TGG 0.557527 8.1:-85342581 MS.gene022779:CDS
TCCCGTTGATCACCTGGCAC+CGG 0.561616 8.1:+85341317 None:intergenic
CTGAATTTGATCCTATCAGA+TGG 0.565796 8.1:-85336442 MS.gene022779:CDS
GAGCAGGCTCAGGACCAGAA+TGG 0.566772 8.1:+85336058 None:intergenic
ATATCCAGCCTTCCGCCACT+GGG 0.566906 8.1:+85335948 None:intergenic
AAGAAACCGAAGCCGGCGGT+TGG 0.567744 8.1:-85341981 MS.gene022779:CDS
CAAACAACCAGGCGAGGCAC+AGG 0.569508 8.1:+85335132 None:intergenic
TTTCGTCTGAGAGTATTTCG+AGG 0.570258 8.1:-85341932 MS.gene022779:CDS
CAACGATGCAGAAAGGGTTG+AGG 0.570273 8.1:+85342405 None:intergenic
TGATTGCGCTGGAAGCAAAA+CGG 0.570525 8.1:+85342356 None:intergenic
TGTTACCAACTCTGTCGTCA+TGG 0.580427 8.1:-85336109 MS.gene022779:CDS
CGAAAGTCCATCCCTTGCAC+CGG 0.580543 8.1:+85341950 None:intergenic
TCGGTGTTATATGTTGCAGA+AGG 0.580711 8.1:+85334972 None:intergenic
TAAAGTAAGCAGTCTCGTCT+GGG 0.587500 8.1:+85336154 None:intergenic
AGAGGAGAGTATGGGAGGAG+TGG 0.589527 8.1:+85342452 None:intergenic
CCGGCAACAACACCAACCGC+CGG 0.590311 8.1:+85341969 None:intergenic
TCTAAGGACATCAAAGTCCA+AGG 0.593017 8.1:-85338213 MS.gene022779:CDS
TGAGGATTGAGCGGCAAGTG+CGG 0.595495 8.1:+85342423 None:intergenic
GGAGGCGAGGTGCACCAGCA+CGG 0.595877 8.1:-85341297 MS.gene022779:CDS
CGGCAAGTGCGGCAACGGAG+AGG 0.599001 8.1:+85342434 None:intergenic
AATAAACGCCATTATTCTTG+CGG 0.600069 8.1:+85341226 None:intergenic
AGATGTTGTTATTGGTCAAG+GGG 0.601195 8.1:-85338041 MS.gene022779:CDS
CGCTAAGAAACCGAAGCCGG+CGG 0.601295 8.1:-85341985 MS.gene022779:CDS
ATTGGTGGACCAGCAACAGA+AGG 0.602880 8.1:-85341206 MS.gene022779:CDS
TAAAGATAATGTCACCTCCA+GGG 0.602937 8.1:+85334940 None:intergenic
CTGAACCATGACGACAGAGT+TGG 0.605660 8.1:+85336104 None:intergenic
AACGGAGAGGAGAGTATGGG+AGG 0.608136 8.1:+85342447 None:intergenic
CAGGTGATCAACGGGAGGCG+AGG 0.609172 8.1:-85341310 MS.gene022779:CDS
AATGTGTGAAATTCTATGAT+GGG 0.609930 8.1:-85340238 MS.gene022779:CDS
GTGAATCCTTGAACACTGGA+TGG 0.610162 8.1:+85339485 None:intergenic
CAGCACGGCGCTGAGCATTG+CGG 0.611241 8.1:-85341282 MS.gene022779:CDS
TCCTTTCATCAAAATGACGA+CGG 0.618852 8.1:-85342609 None:intergenic
GTTCAACCATTGCCCAGTGG+CGG 0.620721 8.1:-85335960 MS.gene022779:CDS
AGAAGCAGCTGCTATAGTCA+TGG 0.621270 8.1:-85337094 MS.gene022779:CDS
ACAGTGATGTCGATTTGTCA+AGG 0.622560 8.1:+85337993 None:intergenic
GTCTTTCAAAATGGAAACGG+AGG 0.624277 8.1:-85337061 MS.gene022779:intron
TCCTATCAGATGGTTGGACA+AGG 0.626498 8.1:-85336432 MS.gene022779:CDS
CGGACAGATCCAGATCTTGG+CGG 0.627606 8.1:+85342376 None:intergenic
AGACAAACTAAATGAAGATG+GGG 0.636091 8.1:+85336364 None:intergenic
ATGCCATGGAACGTAATCCC+CGG 0.637422 8.1:-85342551 MS.gene022779:CDS
CTAAGGACATCAAAGTCCAA+GGG 0.638797 8.1:-85338212 MS.gene022779:CDS
CATGCTGATCACAAACAACC+AGG 0.639186 8.1:+85335121 None:intergenic
AACTGCTGTTGAAAGGACTG+GGG 0.646315 8.1:-85339536 MS.gene022779:CDS
AAGATGGCGTTCGAATGCCA+TGG 0.647403 8.1:-85342565 MS.gene022779:CDS
ATTAAATCTGAATCATCATG+AGG 0.650291 8.1:+85335171 None:intergenic
CTGGTTGTTTGTGATCAGCA+TGG 0.653392 8.1:-85335120 MS.gene022779:CDS
GTTAACGGAATCATATTCAA+CGG 0.655047 8.1:+85342265 None:intergenic
TTCAGTTATCAGAATAACAG+TGG 0.656212 8.1:-85337790 MS.gene022779:intron
TCAAAGCAGCTAGTGCATCA+GGG 0.657552 8.1:-85340212 MS.gene022779:CDS
ACTGCTGTTGAAAGGACTGG+GGG 0.668836 8.1:-85339535 MS.gene022779:CDS
TGATCACAAACAACCAGGCG+AGG 0.670853 8.1:+85335126 None:intergenic
GGCAACGGAGAGGAGAGTAT+GGG 0.670889 8.1:+85342444 None:intergenic
ATGGTTCAACCATTGCCCAG+TGG 0.673212 8.1:-85335963 MS.gene022779:CDS
AGAAAGGGTTGAGGATTGAG+CGG 0.674317 8.1:+85342414 None:intergenic
AATGAATATGAAATCAACGA+AGG 0.675639 8.1:+85336081 None:intergenic
TCTCGATGTCGCAGCCATTG+CGG 0.676574 8.1:-85336034 MS.gene022779:CDS
TGTGGGAATATCGACAACCT+GGG 0.677891 8.1:+85336341 None:intergenic
GATGTTGTTATTGGTCAAGG+GGG 0.679877 8.1:-85338040 MS.gene022779:CDS
GGTGCCAGGTGATCAACGGG+AGG 0.688036 8.1:-85341315 MS.gene022779:CDS
TGACACGTGTGTGATTGAAG+AGG 0.692429 8.1:-85342201 MS.gene022779:CDS
AACTTTCAGTAAGTACAACA+AGG 0.703236 8.1:+85339512 None:intergenic
ACTAGTGCATGGAAGATGTG+TGG 0.704231 8.1:-85338016 MS.gene022779:CDS
ACCGAAGCTTCACTCCCTGG+AGG 0.719612 8.1:-85334954 MS.gene022779:CDS
TTTGATTGGAGTGTAGATGG+CGG 0.774274 8.1:+85342490 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr8.1 gene 85334881 85342618 85334881 ID=MS.gene022779
chr8.1 mRNA 85334881 85342618 85334881 ID=MS.gene022779.t1;Parent=MS.gene022779
chr8.1 exon 85341890 85342618 85341890 ID=MS.gene022779.t1.exon1;Parent=MS.gene022779.t1
chr8.1 CDS 85341890 85342618 85341890 ID=cds.MS.gene022779.t1;Parent=MS.gene022779.t1
chr8.1 exon 85341196 85341369 85341196 ID=MS.gene022779.t1.exon2;Parent=MS.gene022779.t1
chr8.1 CDS 85341196 85341369 85341196 ID=cds.MS.gene022779.t1;Parent=MS.gene022779.t1
chr8.1 exon 85340175 85340336 85340175 ID=MS.gene022779.t1.exon3;Parent=MS.gene022779.t1
chr8.1 CDS 85340175 85340336 85340175 ID=cds.MS.gene022779.t1;Parent=MS.gene022779.t1
chr8.1 exon 85339436 85339578 85339436 ID=MS.gene022779.t1.exon4;Parent=MS.gene022779.t1
chr8.1 CDS 85339436 85339578 85339436 ID=cds.MS.gene022779.t1;Parent=MS.gene022779.t1
chr8.1 exon 85338182 85338257 85338182 ID=MS.gene022779.t1.exon5;Parent=MS.gene022779.t1
chr8.1 CDS 85338182 85338257 85338182 ID=cds.MS.gene022779.t1;Parent=MS.gene022779.t1
chr8.1 exon 85337905 85338080 85337905 ID=MS.gene022779.t1.exon6;Parent=MS.gene022779.t1
chr8.1 CDS 85337905 85338080 85337905 ID=cds.MS.gene022779.t1;Parent=MS.gene022779.t1
chr8.1 exon 85337726 85337807 85337726 ID=MS.gene022779.t1.exon7;Parent=MS.gene022779.t1
chr8.1 CDS 85337726 85337807 85337726 ID=cds.MS.gene022779.t1;Parent=MS.gene022779.t1
chr8.1 exon 85337062 85337139 85337062 ID=MS.gene022779.t1.exon8;Parent=MS.gene022779.t1
chr8.1 CDS 85337062 85337139 85337062 ID=cds.MS.gene022779.t1;Parent=MS.gene022779.t1
chr8.1 exon 85336304 85336465 85336304 ID=MS.gene022779.t1.exon9;Parent=MS.gene022779.t1
chr8.1 CDS 85336304 85336465 85336304 ID=cds.MS.gene022779.t1;Parent=MS.gene022779.t1
chr8.1 exon 85335927 85336190 85335927 ID=MS.gene022779.t1.exon10;Parent=MS.gene022779.t1
chr8.1 CDS 85335927 85336190 85335927 ID=cds.MS.gene022779.t1;Parent=MS.gene022779.t1
chr8.1 exon 85335113 85335217 85335113 ID=MS.gene022779.t1.exon11;Parent=MS.gene022779.t1
chr8.1 CDS 85335113 85335217 85335113 ID=cds.MS.gene022779.t1;Parent=MS.gene022779.t1
chr8.1 exon 85334881 85335021 85334881 ID=MS.gene022779.t1.exon12;Parent=MS.gene022779.t1
chr8.1 CDS 85334881 85335021 85334881 ID=cds.MS.gene022779.t1;Parent=MS.gene022779.t1
Gene Sequence

>MS.gene022779

ATGACGACGGAATTCATCGATCTCGAAACCCAAGATGGCGTTCGAATGCCATGGAACGTAATCCCCGGCACAAAACAAGAATCCACAAACGTCGTCGTTCCCGTCGCCGCCATCTACACTCCAATCAAACACTTCCCTACCATGCCACTCCTCCCATACTCTCCTCTCCGTTGCCGCACTTGCCGCTCAATCCTCAACCCTTTCTGCATCGTTGATTTCGCCGCCAAGATCTGGATCTGTCCGTTTTGCTTCCAGCGCAATCATTTTCCTAATCATTACGCTTCAATCTCTGAAGATAATATGCCTGCTGAGCTTTTTCCTCAATACACTACCGTTGAATATGATTCCGTTAACGATTCTGGACCGCACGTGCCGCCTGTGTTTTTGTTTGTTGTTGACACGTGTGTGATTGAAGAGGAAATTGGTTTTCTTCGATCTGCGATTTCTCAAGCTGTTGAGCTTTTGCCGGATAATTCGCTTGTTGGATTAATTACATTTGGGAGTTTTGTTCATGTTCATGAGTTAGGGTTTGGTATTGTTCCCAAAACTTATGTGTTTAGAGGTAATAAGGATTTGTCTAAGGAGCAGTTGTTGGAGCAGATGAGTTTTTTCGCTAAGAAACCGAAGCCGGCGGTTGGTGTTGTTGCCGGTGCAAGGGATGGACTTTCGTCTGAGAGTATTTCGAGGTTTTTGCTTCCAGCTTCGGAATGTGAATTCGCCCTTAATTCGGTAATGTGCCTGTTGGATTGTTTGGTTTTGTGTTGATCAGGGTTGATAGTGGCAGATTGCGGAAAATAGCAGTATGTTTTGTTCAAATTCCGCTGCTATAGCTGCTATTTAACAACTCTCGCGGTGTTGTGGTGGTATCATGCTATAGTGTAGTAGAATTTGAACAAATTGCTATTGTTCGATGATACACTATTTAACAACACTCATGTAGTGTTGTTAAATAGCAACTATAGCGGTGGTATCATGCTATAGTATAGTATAATTTGAATAAATCGCAATTGTTTCATGATACCCTATTTATTATTTAGTATAAAATGTTGCGGAGTAGCGGCTATAGTGACGCTATGACATTGTTCTCTAGCAGAATTTGAACAATCTACTAGTTTCTGTGATTGATAACATTACACCCGTGGAACAATAGTGATTTGTTTCGATTTTGTTATATTATAGCTGCAATTTGACAACACTGGTATTGATGAATTGAATTGTGTGTGAGTTTTGGTTTGGATTGTTTTTGCAGGTTTTAGATGAGCTGCAGAGGGATCCCTGGCCGGTGCCAGGTGATCAACGGGAGGCGAGGTGCACCAGCACGGCGCTGAGCATTGCGGCTAGTTTGTTGGGTGCTTGTGTTCCTGGTTCTGCCGCAAGAATAATGGCGTTTATTGGTGGACCAGCAACAGAAGGACCTGCTCCTGTAAGCGTTTCTCTTCTTACTTACAGAGTGACAATATAGTTGGTTTTTTTTTTTTTTTTTTTTTTGAAACAGACAATATAGTTGGAAGTATAGAGTAGCTTTACTTGTGTCTAGCCAGCAAAGTAGAGACTAGATAGTAGCTTTTTCTCAAATGCATTGGAAAACGTGTGGATTGAAATCCAAACACATACTATATAGTCAAATTAACTGTGAAAGATTTAGATAGCATCATTTATGGTGTTAGTCCTTTATCATTTTCTACAAGCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTTAAAAAAGATGAAAAATTTCTCTCTCTTTCCCTCTAATCCTCTCCCCCTAAAGCCCTCCCCTCAAATCTCCCAAACATACCCTTAGGCTTTTTTTACATAATAGAATCTACCTTATCATTTGTAAGTTTCTTTCTGACAAAGTTATCCAGTTTCAGTGGATAGCAGCTGTTGTATCTCCATTTACATTTCGTCTTATGATTCTGGAGAGTTTTGTGGTTTCTTTTGTGGTTTTTTTTTCTTCCTCAGCTTTGTCCTTTTGTCGTTGCTTTTGGGCCCTCTCTTGTACTTTTTCTCTGTCTATAGAAATTCATTTTTATCTAAATAAAAATATATGTCGTGGAGGCAGTTGTTCTGTTGTCATTTTGACATTAGTGTGTATGATAATGATTGACCTCTATTTTATGAGTGCCTGTACCAAGGGAATGTATTTGACTGTTTATGATCATACTGTTAGGTATTTCATTTACTTCTACTGATGCTATGGATAGAAATGATTTCCATCTCATTTCATTGGTTGACACTCGACTGTTTCCGTCCTTACGAACTATTTTATATTGTTGTAGATTGTATCCAAACAACTTTCTGAACCAATTCGCTCCCACAAAGATCTGGATAAAGATTCTGTGCCACATTACCATAAATGTGTGAAATTCTATGATGGGCTTTCAAAGCAGCTAGTGCATCAGGGTCATGTTCTAGATCTCTTTGCTTGCGCTCTTGATCAGGTAAAATTTTCTCTTTTTTCAACTGATCTTTTCTGGTTTGCGTACATCATTATGCTTGTTTGTAAGCTGGCAAAGAGATCCTTTAAACCAGTTCACTCATTTTATAATCAGAAGTAGTTACCAAACAATGTTTACAATTTCTTTTCCATGAATATTTTTATATTTGACCAAGTTGTTTTTTTCACCTGTTTTCTTTGTTCTAAAGGCATTCTTGATGTTCCTTGCTTGTAACCTTGTCAACTAAGATTGTAGTTTCATGGGAAAAATTGTTTCATTTTTTTTTACGAAGCTAAGTGTTGTTTTATATGTCGAGGTAATTTTGAAATGATGCCTCCTACAGTCCTACTATTAATAACTTGATTATCCAACTTTATAATTCCTGTTGTTTAAAATCCTTGGCTAATTTCAAGGAGATTAGAATAGAAAAATGTTGAACATTTTGTTAAATGGCCTACTTGATTTTCTTTCTATTGAAATATGTGTTATTGTTTAATTGCTTCAAATGTTTGTAATCATATTCTGGTGGTTGTTTTTGCTTTGTCTAGTGTTCTTATATCTGAAAATATAATTTTTTTGGTCTACTCTTTATCTTTCAGGTTGGCATTGCTGAGCTTAAAACTGCTGTTGAAAGGACTGGGGGCCTTGTTGTACTTACTGAAAGTTTTGGCCATCCAGTGTTCAAGGATTCACTTAAGCGTGTTTTCCAATCTGGTGATTATGATCTGGGCCTAGCATCAAAGTAAGTAATAAAAGATTTTAATTTTTCTATGATATGGATCGTCTTGAATTTCTTGCTTGCATGTGTAGTTAAGTGTTAACCCTACTATTCGTTCTCCCCTTTTATTTGGTACTCGATATTCAGACACTTAAGTTGTTGTCTTTTCTGTCCATGGCCTCCCCAGTGCCACAGATCCATTTAGTCAAGCTTTTCTTGCATCAAAGAATGTTCTTGAGCCTTGTCCTGTCACCAACCTCCATATGCCTAAAGACCCAAATCTTTCTCAAATGACTAGTTCTCTCACCTCTCAAACCTCCTTGTTATTTATATTCAGCATTGTCCATCTCTCAAACAATTCTCACCTGACTAATGGGGTTGTATGCACAAATTTATTATTGTTAGATAAGATACGACTCATTTATATTCGGTGAGCAGTACCAGTTACATTTGTAGATTTTAGGGTTAAGGAATGAGGAAAAAAGATAGATGGCAGACTATAATTGGGACAAGTAAGATGATTTTGGAGTTGATCAGACGTAATTTTAAACATTGTCGAAGGTTGTCTAGAACATGATACCAGTTAAATGGTATATACACGGTGAGTAGTCGACAACCATTATAGTAGGATTCTATTTCTAATTACTGAAGGACTCTATCTCCTGTTAACATAGTAGGATAAAAAGCAAATAATAATGATATCAACAGCCACCTTAATTCTATGATACCATTTTTTTTTTAAGAAGCTAAATTAGCCCACCTAAATTGGCACCGGGGAGACTCGAACCTGAGACCTTGAGGAGGAGCACACTCCCAAGTCCCAAGCCAATACCACCAAGCCAACCCAAGTGGGTTATTCTATGATACCATTTATAACAATAACTGTAGAATTTGAAGAGTATCACATAATAAACACCATGGAAAATAGTCGCCAACAATTTAGGAGTACCCAAACCAAGGTTGCTGGAATAATACAAGTAGGTCTGTGGCATCTGGATCCAGAAACATGATTTCTGGCTATGAATAGTTACGAGGTTACATATTACTTTTTGTCCAAATCTGAAAATCCCTGTATCCAGAAACAAAAGCGTATTCTTTTGGCTTCTGATTCCAGCCAGATACCAGAAACACATGTTCTCTAACCGAAACAAATCAATGAGAAGTTGACTGGCACTTAACCTTATTTTATCTACTCTGTGCAGTGGAATATTTGAGATAAACTGCTCTAAGGACATCAAAGTCCAAGGGATTATTGGCCCTTGTGCTTCTCTTGAAAAGGTATATCTAGAAGTTTTGTTCTATCTTTTTCACATGTTAATTTCCTTATAGCCTCTCCCAAAAGGAAAACAAAATGTTTAGCTGCTTCTTTGTATATGCAGAAAAGCCCATTATGCTCAGATGTTGTTATTGGTCAAGGGGGTACTAGTGCATGGAAGATGTGTGGCCTTGACAAATCGACATCACTGTGCCTGTTTTTTGACATTGTGAGGAAGGAAACTCCAGACGCTACAATGCAATCCACAAGCAATCAATTTTACTTCCAATTCTTGACTTAGTATGAAAACTATCTTAGTGCGCCTTTGAGTAATCTGTTGTCTTTTGTGGTAATGTGGTTGAAATATTTCTCACTCATCACAGATTTTTTGCTTCAGTTATCAGAATAACAGTGGACAAATGAGATTTCGGGTTACAACTCTTTCACGTAGATGGGTTGCTGGACCAGGAAGCATACAGGTAAGCTTTCATCTACCACCATTTGATTCACCAAATTTAGTTATTAAGATATCCATTATTAATACTGCAAAGCAATTCATTTGGGTAATACTGCAAAGCAATTCATTTGGGTTTGTCATGTTGTAACATTTATTATAAAAAATGGTCTTGAGAATTATATACAATGCATATTTAGTTATACATAAGGAATTAACAAAACGAAGACTTTTCTTTGATTATCATTATATTTCTTCCCTTGCATATTTATCAGTAGTTCAGGGAAATTGATGGAGACATCATTTTATGAGCTTATTTTGGATTATTTATACACAATTTTTCTTTAATTGCCCTTTTTATTTTACTCATTTCTTTGCTGAATTGGAGTTTTATGTTTATTGTGTTTGGATAATGACCAATGCTAGTTTTATGTTCATTAACTTTTACAATTAGTTAACGATAGCATGATATGATTTATCTAGTAACATGAGCACTCTATTCATTACTGTAATTACAATTTTTTTCCCACTTGTTTTTAATAATTTCATTATTTAATTCATTTTCTGGAATTCATCAATGTTTTATATCAGTTATATGTAAAATTTTGTAGGATTTGATTTCTGGATTTGACCAAGAAGCAGCTGCTATAGTCATGGCACGCCAAGTGTCTTTCAAAATGGAAACGGAGGTGAGTGCTGGATATTTTCTGCTTCTTTTTAATTGTATTTTGGATAATTGTATCTCAGCCTGTTTGACATGTATTTAGCTGGATATAATCCAAGTCATTGGTTTTTGTTTCTCTTGCAGAAGTTTATTTTCATACACCAGTTAAGAATCATTTTAAAAGGCTCTCTCTATACACACACGCACGCGCAAACACACACACACTTTTTCTGCACCATTTCTCTAACATGCTTTACTTGGATTATTATGGATTATTTTTCTGTAAATTGATTATTCCAGTGGAACATGAGATTTTTCAATAGAAATGGCAATTGCAACATGTATAGTGGCATATGGTTTCCATTTTTTACTCCATTCATGAGAATGAATAATGGAATTCAGTGAATCAGAACCCATTACAGATTACACTTTTGTATTATATTGTTGACTAGTACATTGACACAGAAGTATTCTTTGCCGGTTTGTTTATGACACATCCATAGTTGTGAGAACTGTGGACCTGCTTTTCTCTCTCACTGATTAGCTTGATATGTTTTTGTGCCTGCTTATGAAAAATGTCGTTGTTTTGACACCCTTGTGATGATGCAACTTCTGTATCAGGCTGAATTTGATCCTATCAGATGGTTGGACAAGGCATTGATAAATTTGTGTTCACGTTTCGGAGATTTTCAAAAAGAAACCCCATCTTCATTTAGTTTGTCTCCCAGGTTGTCGATATTCCCACAATTTATGTTTCACTTACGACGTTCTCAGTTTGTTCAGGTATTCATTTTGTGTTTTCAGACCTCTTATAAAGTTTTTCATTGTTGATATTTCGTGCTGCTATATTTGTACTTCAACAGTATTTATTTTTTTCGTTAATTATATAATTGCAGGTCTTCAACAACAGCCCAGACGAGACTGCTTACTTTAGAATGATTTTAAACCGGGAAAATGTTACCAACTCTGTCGTCATGGTTCAGCCTTCGTTGATTTCATATTCATTCCATTCTGGTCCTGAGCCTGCTCTTCTCGATGTCGCAGCCATTGCGGCTGACAGGGTTCTGCTTTTGGATGCATTTTTCACAGTCGTTATTTTTCATGGTTCAACCATTGCCCAGTGGCGGAAGGCTGGATATCATAATGAACCAGAACATCAGGTATTCTCTGGACATGTTATTGCTGATCTAATATCATGTCTGCTAGTTAATTCTGATGAACAAGAGTATCTGGCTGATGGGGGCAATATACATTGTGAAAGTATACGAGAATAGGTGAAATCATTTAGTGTTTTGACACGGTTTCATTGTTGGGATAAATTATACTAGTAGTTTATGGGCTTTACCTGATGATTATGAGTCAAGATTAGGTTGAGTAAGGCGCCTGCTGCTTCGACAAGGACTTTCAGTTTATCGAGCCTTGTATTAGTTTCTAAGACTTTGTGATTTGTTTGTTTGTTAGATTATTTTGGGAGTTGGGGGATGAATAGATTTTGGTAATGAAAGAATCAAAAGAATAAAACATTAATGAACTCAATGTGAATATAAGATCAGTACCACAGCATGTTTTTGTGGAAAACTTTTATTTGATAGAGGTTTTGCAAAATTATACAAATTTTGAACTTCTTAAGCACCCTATCTCAAAGCCTTGATAACTGGAAACATTTAACTATTTGGGGCATAATCAAAAGGTTTTAACTACGAAATCCATGTTATGGCCTGTGCCAAACCTACTTGATAGTTGGATTGGTTGTTGGACACCTTTCCTAAGTCATAATGCATTACTAATGACCATAGGAAATTTGTGAATGAGTAATGATCTTATCTTTGCATTATCAATTTGGTTAACTTTGGTCAAATATTTTTGCAGGCATTTGCTCAGTTGCTACGAGCTCCTCATGATGATTCAGATTTAATAATGAAAGAGAGATTTCCTGTGCCTCGCCTGGTTGTTTGTGATCAGCATGGATCCCAGGTTACATTCTTATACTTTGAACTGACTTGTTGCATATTAGTATAATTTTTTTTGGAATCTGACATGTTTGGCATTCATGCACATTGTGCAGGCTCGATTTCTTCTAGCAAAGTTAAATCCTTCTGCAACATATAACACCGAAGCTTCACTCCCTGGAGGTGACATTATCTTTACGGATGATGTTAGCTTTGACGTCTTCCTTGATCATTTACAGAGATTAGTTGTTCAATAG
Protein sequence

>MS.gene022779.t1

MTTEFIDLETQDGVRMPWNVIPGTKQESTNVVVPVAAIYTPIKHFPTMPLLPYSPLRCRTCRSILNPFCIVDFAAKIWICPFCFQRNHFPNHYASISEDNMPAELFPQYTTVEYDSVNDSGPHVPPVFLFVVDTCVIEEEIGFLRSAISQAVELLPDNSLVGLITFGSFVHVHELGFGIVPKTYVFRGNKDLSKEQLLEQMSFFAKKPKPAVGVVAGARDGLSSESISRFLLPASECEFALNSVLDELQRDPWPVPGDQREARCTSTALSIAASLLGACVPGSAARIMAFIGGPATEGPAPIVSKQLSEPIRSHKDLDKDSVPHYHKCVKFYDGLSKQLVHQGHVLDLFACALDQVGIAELKTAVERTGGLVVLTESFGHPVFKDSLKRVFQSGDYDLGLASNGIFEINCSKDIKVQGIIGPCASLEKKSPLCSDVVIGQGGTSAWKMCGLDKSTSLCLFFDIVRKETPDATMQSTSNQFYFQFLTYYQNNSGQMRFRVTTLSRRWVAGPGSIQDLISGFDQEAAAIVMARQVSFKMETEAEFDPIRWLDKALINLCSRFGDFQKETPSSFSLSPRLSIFPQFMFHLRRSQFVQVFNNSPDETAYFRMILNRENVTNSVVMVQPSLISYSFHSGPEPALLDVAAIAADRVLLLDAFFTVVIFHGSTIAQWRKAGYHNEPEHQAFAQLLRAPHDDSDLIMKERFPVPRLVVCDQHGSQARFLLAKLNPSATYNTEASLPGGDIIFTDDVSFDVFLDHLQRLVVQ