AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene023231

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene023231.t1 AT4G02620 87.302 126 16 0 1 126 1 126 4.46e-80 231

Find 28 sgRNAs with CRISPR-Local

Find 212 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACCATAGTTGGGTTTCTTT+TGG 0.252743 4.3:-17953556 MS.gene023231:CDS
CTCATGATTCAGTTCTTTCA+CGG 0.364488 4.3:-17952061 MS.gene023231:CDS
TTTCCACAGGATACCATAGT+TGG 0.389583 4.3:-17953567 MS.gene023231:intron
GAAGGGATTTCAAGAATTGC+AGG 0.394210 4.3:+17952107 None:intergenic
ATTTCAAGAATTGCAGGAAC+TGG 0.410123 4.3:+17952113 None:intergenic
GAATCATGAGCAGGATCATA+AGG 0.423283 4.3:+17952074 None:intergenic
AGCCAAAAGAAACCCAACTA+TGG 0.431491 4.3:+17953554 None:intergenic
ACTTACTTGAATCCACAATA+AGG 0.438291 4.3:+17953493 None:intergenic
GATGAATTGTTGGCAGGAAT+CGG 0.453548 4.3:+17954820 None:intergenic
GGCAATAAGTGATGAATTGT+TGG 0.454916 4.3:+17954810 None:intergenic
TGCAGGTTGCAAATATGATA+AGG 0.462958 4.3:-17952160 MS.gene023231:intron
GCTTACCTCGTCAGCAATCA+TGG 0.470639 4.3:+17954789 None:intergenic
GAAGACAAATTACCTTATTG+TGG 0.485357 4.3:-17953505 MS.gene023231:CDS
TTCCACAGGATACCATAGTT+GGG 0.495606 4.3:-17953566 MS.gene023231:intron
TTCAATGCTGAATCAGTGGC+AGG 0.511114 4.3:-17952026 MS.gene023231:CDS
TCATGATTCAGTTCTTTCAC+GGG 0.514197 4.3:-17952060 MS.gene023231:CDS
GGCAGGAATCGGAACTCTGT+TGG 0.544048 4.3:+17954831 None:intergenic
ATAAGTGATGAATTGTTGGC+AGG 0.546049 4.3:+17954814 None:intergenic
AATCATGAGCAGGATCATAA+GGG 0.560484 4.3:+17952075 None:intergenic
GAAAGAACTGAATCATGAGC+AGG 0.572900 4.3:+17952065 None:intergenic
TATTGCCATGATTGCTGACG+AGG 0.587291 4.3:-17954794 MS.gene023231:intron
TCTCTTCAATGCTGAATCAG+TGG 0.629677 4.3:-17952030 MS.gene023231:CDS
AACGACTGTCAAACAAATCG+AGG 0.646091 4.3:-17952862 MS.gene023231:CDS
CAAAGAATTCTCAACACGGG+AGG 0.652854 4.3:-17952832 MS.gene023231:CDS
ATTCAAAGAATTCTCAACAC+GGG 0.675761 4.3:-17952835 MS.gene023231:CDS
CATTCAAAGAATTCTCAACA+CGG 0.678390 4.3:-17952836 MS.gene023231:CDS
CATGAGCAGGATCATAAGGG+TGG 0.700765 4.3:+17952078 None:intergenic
TGGGAAACGTTGACATACGT+CGG 0.705364 4.3:-17953527 MS.gene023231:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAAAATGAAAAAATTGTA+TGG + chr4.3:17953434-17953453 None:intergenic 10.0%
!! TTAAAATGAAAAAATTGTAT+GGG + chr4.3:17953433-17953452 None:intergenic 10.0%
!! ACTAGTTATTCAAATTAATA+GGG + chr4.3:17953111-17953130 None:intergenic 15.0%
!! TAAAATGAAAAAATTGTATG+GGG + chr4.3:17953432-17953451 None:intergenic 15.0%
!! TATGAAATGTATTAAAGATA+AGG + chr4.3:17953654-17953673 None:intergenic 15.0%
!! TTAATAAAAAAAGAAATCAG+TGG + chr4.3:17952502-17952521 None:intergenic 15.0%
!! TTACTTACTATTATTTATGA+GGG - chr4.3:17953392-17953411 MS.gene023231:intron 15.0%
!! TTTACTTACTATTATTTATG+AGG - chr4.3:17953391-17953410 MS.gene023231:intron 15.0%
!! AAAATGAAAAAATTGTATGG+GGG + chr4.3:17953431-17953450 None:intergenic 20.0%
!! AAATACCAGCTTATATAATA+TGG + chr4.3:17952730-17952749 None:intergenic 20.0%
!! AATTTGTAAGTCATATTTCA+TGG - chr4.3:17954063-17954082 MS.gene023231:intron 20.0%
!! ATGAAATATGACTTACAAAT+TGG + chr4.3:17954064-17954083 None:intergenic 20.0%
!! CAAAAAAGAAAAATCCAAAA+TGG + chr4.3:17953199-17953218 None:intergenic 20.0%
!! CACTAGTTATTCAAATTAAT+AGG + chr4.3:17953112-17953131 None:intergenic 20.0%
!! CTAGTTATTCAAATTAATAG+GGG + chr4.3:17953110-17953129 None:intergenic 20.0%
!! TAACAGGATTTAAATGAAAT+AGG - chr4.3:17952555-17952574 MS.gene023231:intron 20.0%
!!! AAAATGTTGACTAGTTTATA+TGG - chr4.3:17954472-17954491 MS.gene023231:intron 20.0%
!!! AAATTTAATTTCTTTGGATG+AGG + chr4.3:17952596-17952615 None:intergenic 20.0%
!!! AACACATGTACTATTTTTAT+TGG + chr4.3:17953522-17953541 None:intergenic 20.0%
!!! AATTCAATTTTATTTTCCAC+AGG - chr4.3:17953268-17953287 MS.gene023231:intron 20.0%
!!! AGAGACAAATTTAATTTCTT+TGG + chr4.3:17952602-17952621 None:intergenic 20.0%
!!! ATAAGAAAGTATATACAACA+TGG + chr4.3:17952091-17952110 None:intergenic 20.0%
!!! GGATTTTTCTTTTTTGTAAT+TGG - chr4.3:17953203-17953222 MS.gene023231:intron 20.0%
!!! TAAGAAAGTATATACAACAT+GGG + chr4.3:17952090-17952109 None:intergenic 20.0%
!!! TTAAAGATCTATTTTGCTAT+TGG - chr4.3:17952239-17952258 MS.gene023231:intron 20.0%
!!! TTCAAAACAAATTGATTTGA+CGG - chr4.3:17953926-17953945 MS.gene023231:intron 20.0%
!!! TTTTTGCTAGAATCTATTTA+AGG + chr4.3:17952534-17952553 None:intergenic 20.0%
! AAAATCAGATATAGTTCAGT+CGG - chr4.3:17953064-17953083 MS.gene023231:intron 25.0%
! AAATGAAATAGGAAATTGTG+AGG - chr4.3:17952566-17952585 MS.gene023231:intron 25.0%
! AACATGTGTTAAACTATGAT+AGG + chr4.3:17954224-17954243 None:intergenic 25.0%
! AATCAGAAAATTAGGTGAAA+AGG + chr4.3:17953488-17953507 None:intergenic 25.0%
! AATCAGATGATATGATAACT+AGG - chr4.3:17952139-17952158 MS.gene023231:CDS 25.0%
! AATGAATAATGCTAGATAAC+TGG - chr4.3:17954334-17954353 MS.gene023231:intron 25.0%
! AGAAAAATCCAAAATGGAAT+TGG + chr4.3:17953193-17953212 None:intergenic 25.0%
! AGATTCTAGCAAAAATTAAC+AGG - chr4.3:17952539-17952558 MS.gene023231:intron 25.0%
! AGGATAAGTAAAAAGTCTTT+CGG + chr4.3:17954199-17954218 None:intergenic 25.0%
! ATACTCCTATTTCATTAACA+AGG + chr4.3:17952771-17952790 None:intergenic 25.0%
! CTCTGATAAAATTGAAGAAA+GGG - chr4.3:17952620-17952639 MS.gene023231:intron 25.0%
! GAAAAATCCAAAATGGAATT+GGG + chr4.3:17953192-17953211 None:intergenic 25.0%
! GTGAGATTCAATCATAAAAT+TGG - chr4.3:17954391-17954410 MS.gene023231:intron 25.0%
! TAAATAACCCAAGATTGTAA+AGG + chr4.3:17954646-17954665 None:intergenic 25.0%
! TACTTCCATATTATATAAGC+TGG - chr4.3:17952722-17952741 MS.gene023231:intron 25.0%
! TAGCCAATTACTAGTATAAA+CGG - chr4.3:17952854-17952873 MS.gene023231:CDS 25.0%
! TCAGTTATTAGTTGGATATT+TGG - chr4.3:17954517-17954536 MS.gene023231:intron 25.0%
! TCTCTGATAAAATTGAAGAA+AGG - chr4.3:17952619-17952638 MS.gene023231:intron 25.0%
! TGTATTAAAGATAAGGTTTG+TGG + chr4.3:17953647-17953666 None:intergenic 25.0%
! TTTATGCAAAGGTTATTAGT+AGG - chr4.3:17953730-17953749 MS.gene023231:intron 25.0%
!! AAACCGTAGAATACTTATTT+TGG - chr4.3:17953693-17953712 MS.gene023231:intron 25.0%
!! ACTATTATTGTACATTTTCC+TGG - chr4.3:17952306-17952325 MS.gene023231:intron 25.0%
!! ATTTTATGATTGAATCTCAC+TGG + chr4.3:17954391-17954410 None:intergenic 25.0%
!! TAGATTTGACTTAGAGTAAT+TGG + chr4.3:17952836-17952855 None:intergenic 25.0%
!! TGAAATAGTTTTTAAATCCC+AGG + chr4.3:17954582-17954601 None:intergenic 25.0%
!! TGCAAATATGATAAGGTTTT+TGG - chr4.3:17954695-17954714 MS.gene023231:intron 25.0%
!!! AACCGTAGAATACTTATTTT+GGG - chr4.3:17953694-17953713 MS.gene023231:intron 25.0%
!!! TTTGTGTTTTCCTTATTTCA+GGG + chr4.3:17952882-17952901 None:intergenic 25.0%
!!! TTTTAATTATCTACTCCATC+AGG - chr4.3:17954290-17954309 MS.gene023231:intron 25.0%
AAAAAGAAATCAGTGGCTTT+AGG + chr4.3:17952495-17952514 None:intergenic 30.0%
AAAATCCATACAATGACAGT+TGG - chr4.3:17952915-17952934 MS.gene023231:intron 30.0%
AATGGAATTGGGAAATGATA+TGG + chr4.3:17953181-17953200 None:intergenic 30.0%
ACTTACTTGAATCCACAATA+AGG + chr4.3:17953358-17953377 None:intergenic 30.0%
AGAAAACCTTCTTGCTTATT+TGG - chr4.3:17954620-17954639 MS.gene023231:intron 30.0%
AGACTTATTGTTGTATATCG+TGG - chr4.3:17953558-17953577 MS.gene023231:CDS 30.0%
AGTTTAACACATGTTCTGTT+TGG - chr4.3:17954229-17954248 MS.gene023231:intron 30.0%
ATGGAATTGGGAAATGATAT+GGG + chr4.3:17953180-17953199 None:intergenic 30.0%
ATTCAAAGAATTCTCAACAC+GGG - chr4.3:17954013-17954032 MS.gene023231:intron 30.0%
CATTCAAAGAATTCTCAACA+CGG - chr4.3:17954012-17954031 MS.gene023231:intron 30.0%
CATTTCTACACATCCTATAT+AGG + chr4.3:17953615-17953634 None:intergenic 30.0%
GAAGACAAATTACCTTATTG+TGG - chr4.3:17953343-17953362 MS.gene023231:intron 30.0%
GGTAATCAACACTATTGTTA+GGG - chr4.3:17954250-17954269 MS.gene023231:intron 30.0%
GTGTTAAACTATGATAGGTA+AGG + chr4.3:17954219-17954238 None:intergenic 30.0%
GTTCCGTTTATACTAGTAAT+TGG + chr4.3:17952860-17952879 None:intergenic 30.0%
TAAAAACTATTTCATGCACC+CGG - chr4.3:17954588-17954607 MS.gene023231:intron 30.0%
TAGAAAATAAGTGGATGGAT+AGG - chr4.3:17952401-17952420 MS.gene023231:intron 30.0%
TATAATTTCTTTGACTGTGC+AGG - chr4.3:17954671-17954690 MS.gene023231:intron 30.0%
TCTACCACAATCAGAAAATT+AGG + chr4.3:17953496-17953515 None:intergenic 30.0%
TCTTGAAAGGATCAAATTCA+GGG - chr4.3:17952376-17952395 MS.gene023231:intron 30.0%
TGACTTCTTAAGTCATCTTA+TGG + chr4.3:17952449-17952468 None:intergenic 30.0%
TGCCCAAAATAAGTATTCTA+CGG + chr4.3:17953699-17953718 None:intergenic 30.0%
TGGTAATCAACACTATTGTT+AGG - chr4.3:17954249-17954268 MS.gene023231:intron 30.0%
TTAATTGGTACTTGAACCTT+GGG - chr4.3:17953769-17953788 MS.gene023231:intron 30.0%
TTCAGGGTTTAGAAAATAAG+TGG - chr4.3:17952392-17952411 MS.gene023231:intron 30.0%
! AACTGTCATTGTATGGATTT+TGG + chr4.3:17952916-17952935 None:intergenic 30.0%
! AAGGTTTTCTAAATAGGAAC+CGG + chr4.3:17954610-17954629 None:intergenic 30.0%
! AAGTATTCTACGGTTTTTCT+GGG + chr4.3:17953689-17953708 None:intergenic 30.0%
! AGCAAGAAGGTTTTCTAAAT+AGG + chr4.3:17954616-17954635 None:intergenic 30.0%
! ATAGTTTTTAAATCCCAGGT+AGG + chr4.3:17954578-17954597 None:intergenic 30.0%
! ATCATTTCCCAATTCCATTT+TGG - chr4.3:17953182-17953201 MS.gene023231:intron 30.0%
! CATTAACCTCATGTAAGTTT+TGG + chr4.3:17954318-17954337 None:intergenic 30.0%
! GATTGCCTTGTTAATGAAAT+AGG - chr4.3:17952763-17952782 MS.gene023231:intron 30.0%
! TAAGTATTCTACGGTTTTTC+TGG + chr4.3:17953690-17953709 None:intergenic 30.0%
! TATTAGCTACGATGATTTTG+CGG + chr4.3:17952188-17952207 None:intergenic 30.0%
! TATTTGGCCTTTACAATCTT+GGG - chr4.3:17954636-17954655 MS.gene023231:intron 30.0%
! TTATTTGGCCTTTACAATCT+TGG - chr4.3:17954635-17954654 MS.gene023231:intron 30.0%
! TTCACCTAATTTTCTGATTG+TGG - chr4.3:17953489-17953508 MS.gene023231:intron 30.0%
!! AACAGCCTGAATCATTTTTA+TGG + chr4.3:17954089-17954108 None:intergenic 30.0%
!! GTTTGTGTTTTCCTTATTTC+AGG + chr4.3:17952883-17952902 None:intergenic 30.0%
!! TCAAGTTCTGAAAATATTCC+TGG - chr4.3:17953887-17953906 MS.gene023231:intron 30.0%
!! TGACTAGTTTATATGGTCAT+TGG - chr4.3:17954479-17954498 MS.gene023231:intron 30.0%
!!! AGAATACTTATTTTGGGCAT+GGG - chr4.3:17953700-17953719 MS.gene023231:intron 30.0%
!!! ATTGTATGGATTTTGGCTTT+GGG + chr4.3:17952909-17952928 None:intergenic 30.0%
!!! TAGAATACTTATTTTGGGCA+TGG - chr4.3:17953699-17953718 MS.gene023231:intron 30.0%
!!! TGGGCTATTTTTTTATGCAA+AGG - chr4.3:17953719-17953738 MS.gene023231:intron 30.0%
AAACTGAGCAAGAAATTACC+AGG + chr4.3:17953908-17953927 None:intergenic 35.0%
AATCATGAGCAGGATCATAA+GGG + chr4.3:17954776-17954795 None:intergenic 35.0%
ACCAATTAACCTCAGTTTCA+GGG + chr4.3:17953758-17953777 None:intergenic 35.0%
ACTAGGATGCATGTTTGAAT+AGG - chr4.3:17952156-17952175 MS.gene023231:CDS 35.0%
AGAAAATTAGGTGAAAAGGC+AGG + chr4.3:17953484-17953503 None:intergenic 35.0%
ATAAACGGAACCCTGAAATA+AGG - chr4.3:17952869-17952888 MS.gene023231:intron 35.0%
ATAAGTGATGAATTGTTGGC+AGG + chr4.3:17952037-17952056 None:intergenic 35.0%
ATGTAATAAGCTGATGTCTC+AGG + chr4.3:17954176-17954195 None:intergenic 35.0%
ATTGTTAGGGAAAATCTGGA+GGG - chr4.3:17954263-17954282 MS.gene023231:intron 35.0%
ATTTCAAGAATTGCAGGAAC+TGG + chr4.3:17954738-17954757 None:intergenic 35.0%
CACTATTGTTAGGGAAAATC+TGG - chr4.3:17954259-17954278 MS.gene023231:intron 35.0%
CATGGCCATAAAAATGATTC+AGG - chr4.3:17954081-17954100 MS.gene023231:intron 35.0%
CTAGGATGCATGTTTGAATA+GGG - chr4.3:17952157-17952176 MS.gene023231:CDS 35.0%
CTCATGATTCAGTTCTTTCA+CGG - chr4.3:17954787-17954806 MS.gene023231:intron 35.0%
CTCTTGAAAGGATCAAATTC+AGG - chr4.3:17952375-17952394 MS.gene023231:intron 35.0%
CTGTTGGATCAGTTATTAGT+TGG - chr4.3:17954509-17954528 MS.gene023231:intron 35.0%
GAAAATTAGGTGAAAAGGCA+GGG + chr4.3:17953483-17953502 None:intergenic 35.0%
GAAAGGGATTTAGAGATCAT+AGG - chr4.3:17952636-17952655 MS.gene023231:intron 35.0%
GGCAATAAGTGATGAATTGT+TGG + chr4.3:17952041-17952060 None:intergenic 35.0%
GGGTTTAATAGGTTAGGTTT+AGG - chr4.3:17952350-17952369 MS.gene023231:intron 35.0%
GGGTTTAGAAAATAAGTGGA+TGG - chr4.3:17952396-17952415 MS.gene023231:intron 35.0%
GTTAATTGGTACTTGAACCT+TGG - chr4.3:17953768-17953787 MS.gene023231:intron 35.0%
TAAAGGCCAAATAAGCAAGA+AGG + chr4.3:17954629-17954648 None:intergenic 35.0%
TACCAATTAACCTCAGTTTC+AGG + chr4.3:17953759-17953778 None:intergenic 35.0%
TATTGTTAGGGAAAATCTGG+AGG - chr4.3:17954262-17954281 MS.gene023231:intron 35.0%
TCATGATTCAGTTCTTTCAC+GGG - chr4.3:17954788-17954807 MS.gene023231:intron 35.0%
TGCAGGTTGCAAATATGATA+AGG - chr4.3:17954688-17954707 MS.gene023231:intron 35.0%
TTGTCGACATTTAAACCCAA+AGG + chr4.3:17954436-17954455 None:intergenic 35.0%
! ACTTTCAGTTTTCAGCCATA+GGG + chr4.3:17953008-17953027 None:intergenic 35.0%
! AGGTTTTCTAAATAGGAACC+GGG + chr4.3:17954609-17954628 None:intergenic 35.0%
! AGTATTCTACGGTTTTTCTG+GGG + chr4.3:17953688-17953707 None:intergenic 35.0%
! TACCATAGTTGGGTTTCTTT+TGG - chr4.3:17953292-17953311 MS.gene023231:intron 35.0%
! TACTTTCAGTTTTCAGCCAT+AGG + chr4.3:17953009-17953028 None:intergenic 35.0%
! TAGGTTTAGGTTCTCTTGAA+AGG - chr4.3:17952363-17952382 MS.gene023231:intron 35.0%
! TATTGTACATTTTCCTGGTG+TGG - chr4.3:17952311-17952330 MS.gene023231:intron 35.0%
!! TTTTCTTAAGAAGTGTGCAG+TGG + chr4.3:17952283-17952302 None:intergenic 35.0%
!!! ATGTAAGTTTTGGAACCTGA+TGG + chr4.3:17954308-17954327 None:intergenic 35.0%
!!! CATTGTATGGATTTTGGCTT+TGG + chr4.3:17952910-17952929 None:intergenic 35.0%
!!! TTGTATGGATTTTGGCTTTG+GGG + chr4.3:17952908-17952927 None:intergenic 35.0%
AACGACTGTCAAACAAATCG+AGG - chr4.3:17953986-17954005 MS.gene023231:intron 40.0%
ACACTCCAACTGTCATTGTA+TGG + chr4.3:17952923-17952942 None:intergenic 40.0%
ACCCTGAAACTGAGGTTAAT+TGG - chr4.3:17953754-17953773 MS.gene023231:intron 40.0%
ACCGCACTAAAATATCCCTA+TGG - chr4.3:17952990-17953009 MS.gene023231:intron 40.0%
ACTGCTTGGTACAAATCACA+GGG + chr4.3:17952799-17952818 None:intergenic 40.0%
AGCCAAAAGAAACCCAACTA+TGG + chr4.3:17953297-17953316 None:intergenic 40.0%
AGTAATCCAAGTACTACCCA+AGG + chr4.3:17953788-17953807 None:intergenic 40.0%
ATCTGTGCATCATGTTCATG+TGG - chr4.3:17953452-17953471 MS.gene023231:intron 40.0%
CAATGGCTGATTGAGCTAAA+AGG + chr4.3:17954549-17954568 None:intergenic 40.0%
CAGGTTCCAAAACTTACATG+AGG - chr4.3:17954309-17954328 MS.gene023231:intron 40.0%
CGACATTTAAACCCAAAGGA+TGG + chr4.3:17954432-17954451 None:intergenic 40.0%
CGGCAATTGAACATTACAAC+TGG - chr4.3:17953084-17953103 MS.gene023231:intron 40.0%
CTGTTAGTTCCTACTTCCTA+CGG - chr4.3:17954150-17954169 MS.gene023231:intron 40.0%
GAAAGAACTGAATCATGAGC+AGG + chr4.3:17954786-17954805 None:intergenic 40.0%
GAAGGGATTTCAAGAATTGC+AGG + chr4.3:17954744-17954763 None:intergenic 40.0%
GAATCATGAGCAGGATCATA+AGG + chr4.3:17954777-17954796 None:intergenic 40.0%
GATGAATTGTTGGCAGGAAT+CGG + chr4.3:17952031-17952050 None:intergenic 40.0%
GATTGCATCACACATAGTCA+AGG - chr4.3:17952689-17952708 MS.gene023231:intron 40.0%
GGGATATGTCTCTCCTATAT+AGG - chr4.3:17953599-17953618 MS.gene023231:intron 40.0%
TACAATGACAGTTGGAGTGT+TGG - chr4.3:17952923-17952942 MS.gene023231:intron 40.0%
TAGAAGGCTTGGGGTTTAAT+AGG - chr4.3:17952339-17952358 MS.gene023231:intron 40.0%
TAGGAAGTAGGAACTAACAG+TGG + chr4.3:17954150-17954169 None:intergenic 40.0%
TATCCCCATGCTTATTTACG+AGG + chr4.3:17953585-17953604 None:intergenic 40.0%
TCTCTTCAATGCTGAATCAG+TGG - chr4.3:17954818-17954837 MS.gene023231:CDS 40.0%
TCTTTCGATAGCCATCCTTT+GGG - chr4.3:17954418-17954437 MS.gene023231:intron 40.0%
TGGACCTCGTAAATAAGCAT+GGG - chr4.3:17953578-17953597 MS.gene023231:intron 40.0%
TTCCACAGGATACCATAGTT+GGG - chr4.3:17953282-17953301 MS.gene023231:intron 40.0%
TTCTTTCGATAGCCATCCTT+TGG - chr4.3:17954417-17954436 MS.gene023231:intron 40.0%
TTTCCACAGGATACCATAGT+TGG - chr4.3:17953281-17953300 MS.gene023231:intron 40.0%
! ATAGTTGGGTTTCTTTTGGC+TGG - chr4.3:17953296-17953315 MS.gene023231:intron 40.0%
! CATAAGGGTGGTCTTTTGAA+GGG + chr4.3:17954761-17954780 None:intergenic 40.0%
! TCATAAGGGTGGTCTTTTGA+AGG + chr4.3:17954762-17954781 None:intergenic 40.0%
!!! GCCATAGGGATATTTTAGTG+CGG + chr4.3:17952994-17953013 None:intergenic 40.0%
!!! TGTATGGATTTTGGCTTTGG+GGG + chr4.3:17952907-17952926 None:intergenic 40.0%
AACCCAACTATGGTATCCTG+TGG + chr4.3:17953287-17953306 None:intergenic 45.0%
AAGTAGGAACTAACAGTGGC+AGG + chr4.3:17954146-17954165 None:intergenic 45.0%
AGCCATTGTGATATCCTACC+TGG - chr4.3:17954561-17954580 MS.gene023231:intron 45.0%
ATGGATAGGTTCTCGAGGAA+GGG - chr4.3:17952415-17952434 MS.gene023231:intron 45.0%
CAAAGAATTCTCAACACGGG+AGG - chr4.3:17954016-17954035 MS.gene023231:intron 45.0%
CTTGAACCTTGGGTAGTACT+TGG - chr4.3:17953779-17953798 MS.gene023231:intron 45.0%
GACTGCTTGGTACAAATCAC+AGG + chr4.3:17952800-17952819 None:intergenic 45.0%
GCCATTGTGATATCCTACCT+GGG - chr4.3:17954562-17954581 MS.gene023231:intron 45.0%
GGACCTCGTAAATAAGCATG+GGG - chr4.3:17953579-17953598 MS.gene023231:intron 45.0%
GGCTGATTGAGCTAAAAGGA+CGG + chr4.3:17954545-17954564 None:intergenic 45.0%
GGCTTGGGGTTTAATAGGTT+AGG - chr4.3:17952344-17952363 MS.gene023231:intron 45.0%
GTGGACCTCGTAAATAAGCA+TGG - chr4.3:17953577-17953596 MS.gene023231:intron 45.0%
TAGTAGGTACCCTGAAACTG+AGG - chr4.3:17953746-17953765 MS.gene023231:intron 45.0%
TATTGCCATGATTGCTGACG+AGG - chr4.3:17952054-17952073 MS.gene023231:CDS 45.0%
TCCCAGGTAGGATATCACAA+TGG + chr4.3:17954566-17954585 None:intergenic 45.0%
TCTCACTGGTACTGTCACAT+TGG + chr4.3:17954377-17954396 None:intergenic 45.0%
TGCATCACACATAGTCAAGG+AGG - chr4.3:17952692-17952711 MS.gene023231:intron 45.0%
TGGGAAACGTTGACATACGT+CGG - chr4.3:17953321-17953340 MS.gene023231:intron 45.0%
TTCAATGCTGAATCAGTGGC+AGG - chr4.3:17954822-17954841 MS.gene023231:CDS 45.0%
! TGTGGAGTACTAGAAGGCTT+GGG - chr4.3:17952329-17952348 MS.gene023231:intron 45.0%
!! GGTCATTGGTTTCTCACTGT+TGG - chr4.3:17954493-17954512 MS.gene023231:intron 45.0%
!!! TATTTTAGTGCGGTGCTGCT+AGG + chr4.3:17952984-17953003 None:intergenic 45.0%
ACCTGCAGTTTCAGACTGCT+TGG + chr4.3:17952813-17952832 None:intergenic 50.0%
AGTGGATGGATAGGTTCTCG+AGG - chr4.3:17952410-17952429 MS.gene023231:intron 50.0%
CATGAGCAGGATCATAAGGG+TGG + chr4.3:17954773-17954792 None:intergenic 50.0%
GATGGATAGGTTCTCGAGGA+AGG - chr4.3:17952414-17952433 MS.gene023231:intron 50.0%
GCTTACCTCGTCAGCAATCA+TGG + chr4.3:17952062-17952081 None:intergenic 50.0%
! ACCAAGCAGTCTGAAACTGC+AGG - chr4.3:17952809-17952828 MS.gene023231:CDS 50.0%
! GTGGAGTACTAGAAGGCTTG+GGG - chr4.3:17952330-17952349 MS.gene023231:intron 50.0%
! GTGTGGAGTACTAGAAGGCT+TGG - chr4.3:17952328-17952347 MS.gene023231:intron 50.0%
! TCCTGGTGTGGAGTACTAGA+AGG - chr4.3:17952323-17952342 MS.gene023231:intron 50.0%
!! GGGTTTCTTTTGGCTGGAGT+GGG - chr4.3:17953302-17953321 MS.gene023231:intron 50.0%
!! TGGGTTTCTTTTGGCTGGAG+TGG - chr4.3:17953301-17953320 MS.gene023231:intron 50.0%
AAGCTGATGTCTCAGGCCGT+AGG + chr4.3:17954169-17954188 None:intergenic 55.0%
GCCTTCTAGTACTCCACACC+AGG + chr4.3:17952327-17952346 None:intergenic 55.0%
GGCAGGAATCGGAACTCTGT+TGG + chr4.3:17952020-17952039 None:intergenic 55.0%
TGTCTCAGGCCGTAGGAAGT+AGG + chr4.3:17954162-17954181 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 17952013 17954857 17952013 ID=MS.gene023231
chr4.3 mRNA 17952013 17954857 17952013 ID=MS.gene023231.t1;Parent=MS.gene023231
chr4.3 exon 17954795 17954857 17954795 ID=MS.gene023231.t1.exon1;Parent=MS.gene023231.t1
chr4.3 CDS 17954795 17954857 17954795 ID=cds.MS.gene023231.t1;Parent=MS.gene023231.t1
chr4.3 exon 17953499 17953580 17953499 ID=MS.gene023231.t1.exon2;Parent=MS.gene023231.t1
chr4.3 CDS 17953499 17953580 17953499 ID=cds.MS.gene023231.t1;Parent=MS.gene023231.t1
chr4.3 exon 17952803 17952885 17952803 ID=MS.gene023231.t1.exon3;Parent=MS.gene023231.t1
chr4.3 CDS 17952803 17952885 17952803 ID=cds.MS.gene023231.t1;Parent=MS.gene023231.t1
chr4.3 exon 17952013 17952177 17952013 ID=MS.gene023231.t1.exon4;Parent=MS.gene023231.t1
chr4.3 CDS 17952013 17952177 17952013 ID=cds.MS.gene023231.t1;Parent=MS.gene023231.t1
Gene Sequence

>MS.gene023231

ATGGCCAACAGAGTTCCGATTCCTGCCAACAATTCATCACTTATTGCCATGATTGCTGACGAGGTAAGCGCGTGCCCATGTTGTATATACTTTCTTATCAGTGATATGTTGTCTAAAAGAATGTCAAATCAGATGATATGATAACTAGGATGCATGTTTGAATAGGGTTTAGCCGCAAAATCATCGTAGCTAATATAAGTTCGCTGCTACAGAATTTTGTGATATTTTAAAGATCTATTTTGCTATTGGCGTACAACTCGTTATGCACCACTGCACACTTCTTAAGAAAACAAACTATTATTGTACATTTTCCTGGTGTGGAGTACTAGAAGGCTTGGGGTTTAATAGGTTAGGTTTAGGTTCTCTTGAAAGGATCAAATTCAGGGTTTAGAAAATAAGTGGATGGATAGGTTCTCGAGGAAGGGACTTTCAGCCATAAGATGACTTAAGAAGTCAGATTGACGACATTTCAGATGCTGCCTAAAGCCACTGATTTCTTTTTTTATTAATTGAGTTTTCCTTAAATAGATTCTAGCAAAAATTAACAGGATTTAAATGAAATAGGAAATTGTGAGGTCGTCCTCATCCAAAGAAATTAAATTTGTCTCTCTGATAAAATTGAAGAAAGGGATTTAGAGATCATAGGAAATGAAGAAAATCATCATACATTGAGTTTGATTGCATCACACATAGTCAAGGAGGAGCAATATACTTCCATATTATATAAGCTGGTATTTTATTGATGTTTTTGATTGCCTTGTTAATGAAATAGGAGTATAAGTACCCTGTGATTTGTACCAAGCAGTCTGAAACTGCAGGTCCAATTACTCTAAGTCAAATCTAGCCAATTACTAGTATAAACGGAACCCTGAAATAAGGAAAACACAAACTCCCCCAAAGCCAAAATCCATACAATGACAGTTGGAGTGTTGGACATGAGTATATGACTAGATACTTAACTATACAGTCCTAGCAGCACCGCACTAAAATATCCCTATGGCTGAAAACTGAAAGTATAATAATCTTTTGACATTATAAGTTTTCTAATCTTAAAATCAGATATAGTTCAGTCGGCAATTGAACATTACAACTGGCCCCTATTAATTTGAATAACTAGTGAGATATTATATTCTTTGAGCTTCTCTTTTTTATATTCTAATTTTGCCCATATCATTTCCCAATTCCATTTTGGATTTTTCTTTTTTGTAATTGGTATTCATTATGATTGAAATGATTAAAATGTTCTGAGATTTTGAATTCAATTTTATTTTCCACAGGATACCATAGTTGGGTTTCTTTTGGCTGGAGTGGGAAACGTTGACATACGTCGGAAGACAAATTACCTTATTGTGGATTCAAGTAAGTCACTAAACTATATTTTACTTACTATTATTTATGAGGGCTAGTTTCGAATTCCCCCATACAATTTTTTCATTTTAATCTGTGCATCATGTTCATGTGGAGACTCCCTGCCTTTTCACCTAATTTTCTGATTGTGGTAGATGTCCAATAAAAATAGTACATGTGTTCTTTTATGTTTTATAAAGACTTATTGTTGTATATCGTGGACCTCGTAAATAAGCATGGGGATATGTCTCTCCTATATAGGATGTGTAGAAATGTTGTGATTTCCACAAACCTTATCTTTAATACATTTCATAGCTTAGACACTCCCCAGAAAAACCGTAGAATACTTATTTTGGGCATGGGCTATTTTTTTATGCAAAGGTTATTAGTAGGTACCCTGAAACTGAGGTTAATTGGTACTTGAACCTTGGGTAGTACTTGGATTACTCACTACAGATTGATAAGATAGCGTCATTTTTTTATTTTTATTTTAAATATAGCATCATTTTTTCTCTCTGAATAGTGTGTCAAGTTCTGAAAATATTCCTGGTAATTTCTTGCTCAGTTTCAAAACAAATTGATTTGACGGAAATTTTTTGTTTTAACTCTTGATTTTGATCTTCAGAAACGACTGTCAAACAAATCGAGGATGCATTCAAAGAATTCTCAACACGGGAGGATATTGCAATTGTGCTGATTAGCCAATTTGTAAGTCATATTTCATGGCCATAAAAATGATTCAGGCTGTTTTGTTCTTGCTTCTTAGTTAAGTAAAACACTATACCTGCCACTGTTAGTTCCTACTTCCTACGGCCTGAGACATCAGCTTATTACATCCGAAAGACTTTTTACTTATCCTTACCTATCATAGTTTAACACATGTTCTGTTTGGTAATCAACACTATTGTTAGGGAAAATCTGGAGGGAATATTTTAATTATCTACTCCATCAGGTTCCAAAACTTACATGAGGTTAATGAATAATGCTAGATAACTGGAGATTTTATTTGACAGACCAATGTGACAGTACCAGTGAGATTCAATCATAAAATTGGCACTTCTTTCGATAGCCATCCTTTGGGTTTAAATGTCGACAAGTTATATTATTGCAAAAAAATGTTGACTAGTTTATATGGTCATTGGTTTCTCACTGTTGGATCAGTTATTAGTTGGATATTTGGATCCGTCCTTTTAGCTCAATCAGCCATTGTGATATCCTACCTGGGATTTAAAAACTATTTCATGCACCCGGTTCCTATTTAGAAAACCTTCTTGCTTATTTGGCCTTTACAATCTTGGGTTATTTATATCTTATAATTTCTTTGACTGTGCAGGTTGCAAATATGATAAGGTTTTTGGTTGATAGTTATAACAAGCCAGTTCCTGCAATTCTTGAAATCCCTTCAAAAGACCACCCTTATGATCCTGCTCATGATTCAGTTCTTTCACGGGTGAAGTATCTCTTCAATGCTGAATCAGTGGCAGGTGATAGACGTTGA
Protein sequence

>MS.gene023231.t1

MANRVPIPANNSSLIAMIADEDTIVGFLLAGVGNVDIRRKTNYLIVDSKTTVKQIEDAFKEFSTREDIAIVLISQFVANMIRFLVDSYNKPVPAILEIPSKDHPYDPAHDSVLSRVKYLFNAESVAGDRR