Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22436.t1 | XP_003625625.1 | 95.5 | 110 | 5 | 0 | 1 | 110 | 1 | 110 | 1.70E-45 | 191.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22436.t1 | O82628 | 73.6 | 110 | 29 | 0 | 1 | 110 | 1 | 110 | 1.4e-32 | 140.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22436.t1 | Q2HV42 | 95.5 | 110 | 5 | 0 | 1 | 110 | 1 | 110 | 1.2e-45 | 191.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene22436 | 0.809383 | 1.94E-50 | -1.69E-46 |
| MS.gene049341 | MS.gene22436 | 0.833674 | 4.66E-56 | -1.69E-46 |
| MS.gene049350 | MS.gene22436 | 0.817473 | 3.24E-52 | -1.69E-46 |
| MS.gene049807 | MS.gene22436 | 0.844705 | 6.41E-59 | -1.69E-46 |
| MS.gene049821 | MS.gene22436 | 0.823443 | 1.38E-53 | -1.69E-46 |
| MS.gene049824 | MS.gene22436 | 0.807154 | 5.81E-50 | -1.69E-46 |
| MS.gene049825 | MS.gene22436 | 0.80649 | 8.02E-50 | -1.69E-46 |
| MS.gene049887 | MS.gene22436 | 0.846788 | 1.74E-59 | -1.69E-46 |
| MS.gene050807 | MS.gene22436 | 0.829538 | 4.87E-55 | -1.69E-46 |
| MS.gene050808 | MS.gene22436 | 0.805892 | 1.07E-49 | -1.69E-46 |
| MS.gene050838 | MS.gene22436 | 0.865261 | 6.71E-65 | -1.69E-46 |
| MS.gene050901 | MS.gene22436 | 0.803444 | 3.48E-49 | -1.69E-46 |
| MS.gene051102 | MS.gene22436 | 0.802701 | 4.95E-49 | -1.69E-46 |
| MS.gene051247 | MS.gene22436 | 0.819971 | 8.77E-53 | -1.69E-46 |
| MS.gene051328 | MS.gene22436 | 0.813373 | 2.64E-51 | -1.69E-46 |
| MS.gene051687 | MS.gene22436 | 0.843123 | 1.70E-58 | -1.69E-46 |
| MS.gene051700 | MS.gene22436 | 0.838026 | 3.67E-57 | -1.69E-46 |
| MS.gene051719 | MS.gene22436 | 0.84689 | 1.63E-59 | -1.69E-46 |
| MS.gene051720 | MS.gene22436 | 0.833614 | 4.82E-56 | -1.69E-46 |
| MS.gene051746 | MS.gene22436 | 0.817298 | 3.55E-52 | -1.69E-46 |
| MS.gene051748 | MS.gene22436 | 0.811461 | 6.92E-51 | -1.69E-46 |
| MS.gene051850 | MS.gene22436 | 0.813189 | 2.90E-51 | -1.69E-46 |
| MS.gene051885 | MS.gene22436 | 0.832476 | 9.25E-56 | -1.69E-46 |
| MS.gene051903 | MS.gene22436 | 0.807668 | 4.52E-50 | -1.69E-46 |
| MS.gene052032 | MS.gene22436 | 0.804093 | 2.55E-49 | -1.69E-46 |
| MS.gene052133 | MS.gene22436 | 0.878265 | 3.23E-69 | -1.69E-46 |
| MS.gene052335 | MS.gene22436 | 0.835374 | 1.74E-56 | -1.69E-46 |
| MS.gene052516 | MS.gene22436 | 0.818234 | 2.18E-52 | -1.69E-46 |
| MS.gene052665 | MS.gene22436 | 0.840177 | 1.02E-57 | -1.69E-46 |
| MS.gene052732 | MS.gene22436 | 0.833303 | 5.76E-56 | -1.69E-46 |
| MS.gene052879 | MS.gene22436 | 0.819204 | 1.31E-52 | -1.69E-46 |
| MS.gene052947 | MS.gene22436 | 0.857202 | 1.92E-62 | -1.69E-46 |
| MS.gene053144 | MS.gene22436 | 0.860604 | 1.84E-63 | -1.69E-46 |
| MS.gene053171 | MS.gene22436 | 0.808327 | 3.27E-50 | -1.69E-46 |
| MS.gene053437 | MS.gene22436 | 0.807771 | 4.30E-50 | -1.69E-46 |
| MS.gene053581 | MS.gene22436 | 0.878799 | 2.10E-69 | -1.69E-46 |
| MS.gene053597 | MS.gene22436 | 0.800373 | 1.49E-48 | -1.69E-46 |
| MS.gene053599 | MS.gene22436 | 0.825556 | 4.40E-54 | -1.69E-46 |
| MS.gene05364 | MS.gene22436 | 0.807063 | 6.07E-50 | -1.69E-46 |
| MS.gene053677 | MS.gene22436 | 0.803179 | 3.94E-49 | -1.69E-46 |
| MS.gene053678 | MS.gene22436 | 0.824274 | 8.82E-54 | -1.69E-46 |
| MS.gene054250 | MS.gene22436 | 0.82434 | 8.51E-54 | -1.69E-46 |
| MS.gene054584 | MS.gene22436 | 0.852431 | 4.64E-61 | -1.69E-46 |
| MS.gene05462 | MS.gene22436 | 0.802752 | 4.83E-49 | -1.69E-46 |
| MS.gene055424 | MS.gene22436 | 0.804685 | 1.92E-49 | -1.69E-46 |
| MS.gene055465 | MS.gene22436 | 0.860742 | 1.67E-63 | -1.69E-46 |
| MS.gene055466 | MS.gene22436 | 0.856124 | 3.98E-62 | -1.69E-46 |
| MS.gene055468 | MS.gene22436 | 0.881255 | 2.79E-70 | -1.69E-46 |
| MS.gene055469 | MS.gene22436 | 0.848908 | 4.54E-60 | -1.69E-46 |
| MS.gene055537 | MS.gene22436 | 0.824162 | 9.37E-54 | -1.69E-46 |
| MS.gene055580 | MS.gene22436 | -0.866515 | 2.69E-65 | -1.69E-46 |
| MS.gene055890 | MS.gene22436 | 0.821276 | 4.40E-53 | -1.69E-46 |
| MS.gene055891 | MS.gene22436 | 0.86201 | 6.86E-64 | -1.69E-46 |
| MS.gene056043 | MS.gene22436 | -0.864254 | 1.39E-64 | -1.69E-46 |
| MS.gene056075 | MS.gene22436 | 0.828677 | 7.87E-55 | -1.69E-46 |
| MS.gene056215 | MS.gene22436 | 0.804624 | 1.98E-49 | -1.69E-46 |
| MS.gene056308 | MS.gene22436 | 0.818585 | 1.82E-52 | -1.69E-46 |
| MS.gene056432 | MS.gene22436 | 0.819068 | 1.41E-52 | -1.69E-46 |
| MS.gene056584 | MS.gene22436 | 0.80104 | 1.09E-48 | -1.69E-46 |
| MS.gene057057 | MS.gene22436 | 0.806364 | 8.53E-50 | -1.69E-46 |
| MS.gene057094 | MS.gene22436 | 0.800564 | 1.36E-48 | -1.69E-46 |
| MS.gene057234 | MS.gene22436 | 0.801396 | 9.19E-49 | -1.69E-46 |
| MS.gene057705 | MS.gene22436 | 0.824929 | 6.18E-54 | -1.69E-46 |
| MS.gene058055 | MS.gene22436 | 0.801008 | 1.10E-48 | -1.69E-46 |
| MS.gene058852 | MS.gene22436 | 0.868157 | 8.04E-66 | -1.69E-46 |
| MS.gene058906 | MS.gene22436 | 0.839581 | 1.45E-57 | -1.69E-46 |
| MS.gene058945 | MS.gene22436 | 0.823517 | 1.33E-53 | -1.69E-46 |
| MS.gene059171 | MS.gene22436 | 0.843552 | 1.31E-58 | -1.69E-46 |
| MS.gene059227 | MS.gene22436 | 0.836943 | 6.96E-57 | -1.69E-46 |
| MS.gene06001 | MS.gene22436 | 0.84656 | 2.01E-59 | -1.69E-46 |
| MS.gene060194 | MS.gene22436 | 0.805539 | 1.27E-49 | -1.69E-46 |
| MS.gene060429 | MS.gene22436 | 0.821883 | 3.19E-53 | -1.69E-46 |
| MS.gene060587 | MS.gene22436 | 0.854062 | 1.58E-61 | -1.69E-46 |
| MS.gene061006 | MS.gene22436 | 0.806614 | 7.55E-50 | -1.69E-46 |
| MS.gene061100 | MS.gene22436 | 0.804562 | 2.04E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene22436 | MS.gene20061 | PPI |
| MS.gene35221 | MS.gene22436 | PPI |
| MS.gene029841 | MS.gene22436 | PPI |
| MS.gene023231 | MS.gene22436 | PPI |
| MS.gene22436 | MS.gene007513 | PPI |
| MS.gene22436 | MS.gene007434 | PPI |
| MS.gene007434 | MS.gene22436 | PPI |
| MS.gene22436 | MS.gene08393 | PPI |
| MS.gene028232 | MS.gene22436 | PPI |
| MS.gene22436 | MS.gene45416 | PPI |
| MS.gene22436 | MS.gene24597 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22436.t1 | MTR_7g101170 | 95.455 | 110 | 5 | 0 | 1 | 110 | 1 | 110 | 5.14e-72 | 209 |
| MS.gene22436.t1 | MTR_1g064540 | 81.308 | 107 | 20 | 0 | 3 | 109 | 5 | 111 | 4.43e-55 | 167 |
| MS.gene22436.t1 | MTR_7g007770 | 55.046 | 109 | 49 | 0 | 1 | 109 | 42 | 150 | 1.73e-36 | 121 |
| MS.gene22436.t1 | MTR_1g064540 | 77.500 | 80 | 18 | 0 | 30 | 109 | 10 | 89 | 3.65e-36 | 118 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22436.t1 | AT4G23710 | 69.903 | 103 | 31 | 0 | 8 | 110 | 4 | 106 | 2.20e-44 | 140 |
| MS.gene22436.t1 | AT3G01390 | 73.636 | 110 | 29 | 0 | 1 | 110 | 1 | 110 | 5.05e-42 | 134 |
| MS.gene22436.t1 | AT3G01390 | 73.636 | 110 | 29 | 0 | 1 | 110 | 1 | 110 | 5.05e-42 | 134 |
| MS.gene22436.t1 | AT3G01390 | 73.636 | 110 | 29 | 0 | 1 | 110 | 1 | 110 | 5.05e-42 | 134 |
| MS.gene22436.t1 | AT3G01390 | 73.636 | 110 | 29 | 0 | 1 | 110 | 1 | 110 | 5.05e-42 | 134 |
| MS.gene22436.t1 | AT4G25950 | 54.206 | 107 | 47 | 1 | 1 | 107 | 1 | 105 | 1.07e-33 | 113 |
| MS.gene22436.t1 | AT4G25950 | 58.462 | 65 | 27 | 0 | 1 | 65 | 1 | 65 | 3.55e-19 | 75.5 |
Find 18 sgRNAs with CRISPR-Local
Find 47 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACCGAGGCTAGTAGAATTTC+GGG | 0.229288 | 7.1:-8554906 | MS.gene22436:CDS |
| GACCGAGGCTAGTAGAATTT+CGG | 0.295846 | 7.1:-8554907 | MS.gene22436:CDS |
| CACTAAACATCGAGCTCATT+TGG | 0.305668 | 7.1:-8555128 | MS.gene22436:CDS |
| AGGTGGAATTCAACAATTAT+TGG | 0.361539 | 7.1:-8555611 | MS.gene22436:CDS |
| AATTCCACCTTGACTGTTGT+TGG | 0.367869 | 7.1:+8555624 | None:intergenic |
| ATACAGAGCAGTGGAGATTC+TGG | 0.378837 | 7.1:-8554984 | MS.gene22436:intron |
| AGCCAAAGAAGAGGCTGAAA+AGG | 0.415151 | 7.1:-8555155 | MS.gene22436:CDS |
| CCAAGAAGTCTAGTTCTTCA+CGG | 0.458780 | 7.1:+8554848 | None:intergenic |
| CCCCGAAATTCTACTAGCCT+CGG | 0.490166 | 7.1:+8554905 | None:intergenic |
| TAGCCTCGGTCTTCAGCTGA+TGG | 0.496170 | 7.1:+8554919 | None:intergenic |
| ATGGCATCCAACAACAGTCA+AGG | 0.643899 | 7.1:-8555631 | MS.gene22436:CDS |
| GATCCATCAGCTGAAGACCG+AGG | 0.646369 | 7.1:-8554922 | MS.gene22436:CDS |
| CTTCAATGAATACAGAGCAG+TGG | 0.651983 | 7.1:-8554993 | MS.gene22436:intron |
| GTTGAAACAAGCCAAAGAAG+AGG | 0.654824 | 7.1:-8555164 | MS.gene22436:CDS |
| GCATCCAACAACAGTCAAGG+TGG | 0.657348 | 7.1:-8555628 | MS.gene22436:CDS |
| CGAGGCTAGTAGAATTTCGG+GGG | 0.666069 | 7.1:-8554904 | MS.gene22436:CDS |
| CCGAGGCTAGTAGAATTTCG+GGG | 0.697212 | 7.1:-8554905 | MS.gene22436:CDS |
| CTGCTGAACAAGAAGCACAG+CGG | 0.743446 | 7.1:-8555588 | MS.gene22436:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAACAGTATAATTAAAAAG+TGG | + | chr7.1:8555202-8555221 | None:intergenic | 15.0% |
| !! | AAACAGTATAATTAAAAAGT+GGG | + | chr7.1:8555201-8555220 | None:intergenic | 15.0% |
| !! | GAGAAATAAAAAATGAAGAT+TGG | + | chr7.1:8554957-8554976 | None:intergenic | 20.0% |
| ! | AACAATCTGCTAACATAAAA+TGG | + | chr7.1:8555018-8555037 | None:intergenic | 25.0% |
| ! | AAGCTTATAATGAGATAGAA+CGG | + | chr7.1:8555466-8555485 | None:intergenic | 25.0% |
| ! | AGTATAATTAAAAAGTGGGA+GGG | + | chr7.1:8555197-8555216 | None:intergenic | 25.0% |
| ! | ATTCAGCATAATTACATAAC+TGG | + | chr7.1:8555246-8555265 | None:intergenic | 25.0% |
| ! | GTTAAAAGATAAACACAAGA+AGG | + | chr7.1:8555055-8555074 | None:intergenic | 25.0% |
| ! | TACTGTTAGAAAAATATGCT+AGG | - | chr7.1:8555302-8555321 | MS.gene22436:intron | 25.0% |
| ! | TTTGTTCTATGATATATACG+AGG | + | chr7.1:8555138-8555157 | None:intergenic | 25.0% |
| !! | TTTAACTTTCACATGAAACT+GGG | - | chr7.1:8555069-8555088 | MS.gene22436:intron | 25.0% |
| !!! | TTTTAACTTTCACATGAAAC+TGG | - | chr7.1:8555068-8555087 | MS.gene22436:intron | 25.0% |
| !!! | TTTTCAAAACAAAGTATCAG+CGG | - | chr7.1:8555390-8555409 | MS.gene22436:intron | 25.0% |
| AGCATAATTACATAACTGGT+TGG | + | chr7.1:8555242-8555261 | None:intergenic | 30.0% | |
| AGGTGGAATTCAACAATTAT+TGG | - | chr7.1:8554877-8554896 | MS.gene22436:CDS | 30.0% | |
| ATTGTGAATGCTGCAAAAAA+TGG | - | chr7.1:8554923-8554942 | MS.gene22436:CDS | 30.0% | |
| CAGTATAATTAAAAAGTGGG+AGG | + | chr7.1:8555198-8555217 | None:intergenic | 30.0% | |
| ! | TTATCGACTGCAGTAATAAA+TGG | - | chr7.1:8555421-8555440 | MS.gene22436:intron | 30.0% |
| AATTAAAAAGTGGGAGGGAT+GGG | + | chr7.1:8555192-8555211 | None:intergenic | 35.0% | |
| TAATTAAAAAGTGGGAGGGA+TGG | + | chr7.1:8555193-8555212 | None:intergenic | 35.0% | |
| !! | ATTGGTCTTATGATGGGAAT+GGG | - | chr7.1:8555094-8555113 | MS.gene22436:intron | 35.0% |
| !! | TATTGGTCTTATGATGGGAA+TGG | - | chr7.1:8555093-8555112 | MS.gene22436:intron | 35.0% |
| AATTCCACCTTGACTGTTGT+TGG | + | chr7.1:8554867-8554886 | None:intergenic | 40.0% | |
| ATTAAAAAGTGGGAGGGATG+GGG | + | chr7.1:8555191-8555210 | None:intergenic | 40.0% | |
| CACTAAACATCGAGCTCATT+TGG | - | chr7.1:8555360-8555379 | MS.gene22436:intron | 40.0% | |
| CTTCAATGAATACAGAGCAG+TGG | - | chr7.1:8555495-8555514 | MS.gene22436:intron | 40.0% | |
| GAAGGAAGACAGGTTTATCA+TGG | + | chr7.1:8555169-8555188 | None:intergenic | 40.0% | |
| GTTGAAACAAGCCAAAGAAG+AGG | - | chr7.1:8555324-8555343 | MS.gene22436:intron | 40.0% | |
| TATGATATATACGAGGAGCG+AGG | + | chr7.1:8555131-8555150 | None:intergenic | 40.0% | |
| TTCACATGAAACTGGGCTAT+TGG | - | chr7.1:8555076-8555095 | MS.gene22436:intron | 40.0% | |
| !! | TGGGCTATTGGTCTTATGAT+GGG | - | chr7.1:8555088-8555107 | MS.gene22436:intron | 40.0% |
| ACCGAGGCTAGTAGAATTTC+GGG | - | chr7.1:8555582-8555601 | MS.gene22436:CDS | 45.0% | |
| AGCCAAAGAAGAGGCTGAAA+AGG | - | chr7.1:8555333-8555352 | MS.gene22436:intron | 45.0% | |
| ATACAGAGCAGTGGAGATTC+TGG | - | chr7.1:8555504-8555523 | MS.gene22436:intron | 45.0% | |
| ATGGCATCCAACAACAGTCA+AGG | - | chr7.1:8554857-8554876 | MS.gene22436:CDS | 45.0% | |
| GACCGAGGCTAGTAGAATTT+CGG | - | chr7.1:8555581-8555600 | MS.gene22436:CDS | 45.0% | |
| ! | CTCCTTTTCAGCCTCTTCTT+TGG | + | chr7.1:8555338-8555357 | None:intergenic | 45.0% |
| !! | CTGGGCTATTGGTCTTATGA+TGG | - | chr7.1:8555087-8555106 | MS.gene22436:intron | 45.0% |
| CCGAGGCTAGTAGAATTTCG+GGG | - | chr7.1:8555583-8555602 | MS.gene22436:CDS | 50.0% | |
| CGAGGCTAGTAGAATTTCGG+GGG | - | chr7.1:8555584-8555603 | MS.gene22436:CDS | 50.0% | |
| GCATCCAACAACAGTCAAGG+TGG | - | chr7.1:8554860-8554879 | MS.gene22436:CDS | 50.0% | |
| ! | CCCCGAAATTCTACTAGCCT+CGG | + | chr7.1:8555586-8555605 | None:intergenic | 50.0% |
| ! | CTGCTGAACAAGAAGCACAG+CGG | - | chr7.1:8554900-8554919 | MS.gene22436:CDS | 50.0% |
| AAAAGTGGGAGGGATGGGGA+AGG | + | chr7.1:8555187-8555206 | None:intergenic | 55.0% | |
| GATCCATCAGCTGAAGACCG+AGG | - | chr7.1:8555566-8555585 | MS.gene22436:CDS | 55.0% | |
| TAGCCTCGGTCTTCAGCTGA+TGG | + | chr7.1:8555572-8555591 | None:intergenic | 55.0% | |
| GAGGGATGGGGAAGGAAGAC+AGG | + | chr7.1:8555179-8555198 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 8554857 | 8555653 | 8554857 | ID=MS.gene22436 |
| chr7.1 | mRNA | 8554857 | 8555653 | 8554857 | ID=MS.gene22436.t1;Parent=MS.gene22436 |
| chr7.1 | exon | 8555566 | 8555653 | 8555566 | ID=MS.gene22436.t1.exon1;Parent=MS.gene22436.t1 |
| chr7.1 | CDS | 8555566 | 8555653 | 8555566 | ID=cds.MS.gene22436.t1;Parent=MS.gene22436.t1 |
| chr7.1 | exon | 8555099 | 8555199 | 8555099 | ID=MS.gene22436.t1.exon2;Parent=MS.gene22436.t1 |
| chr7.1 | CDS | 8555099 | 8555199 | 8555099 | ID=cds.MS.gene22436.t1;Parent=MS.gene22436.t1 |
| chr7.1 | exon | 8554857 | 8555000 | 8554857 | ID=MS.gene22436.t1.exon3;Parent=MS.gene22436.t1 |
| chr7.1 | CDS | 8554857 | 8555000 | 8554857 | ID=cds.MS.gene22436.t1;Parent=MS.gene22436.t1 |
| Gene Sequence |
| Protein sequence |
