Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20061.t1 | XP_003625625.1 | 95.5 | 110 | 5 | 0 | 1 | 110 | 1 | 110 | 1.70E-45 | 191.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20061.t1 | O82628 | 73.6 | 110 | 29 | 0 | 1 | 110 | 1 | 110 | 1.4e-32 | 140.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20061.t1 | Q2HV42 | 95.5 | 110 | 5 | 0 | 1 | 110 | 1 | 110 | 1.2e-45 | 191.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene20061 | 0.809308 | 2.02E-50 | -1.69E-46 |
| MS.gene049341 | MS.gene20061 | 0.823382 | 1.43E-53 | -1.69E-46 |
| MS.gene049350 | MS.gene20061 | 0.827293 | 1.69E-54 | -1.69E-46 |
| MS.gene049807 | MS.gene20061 | 0.844085 | 9.41E-59 | -1.69E-46 |
| MS.gene049821 | MS.gene20061 | 0.833415 | 5.40E-56 | -1.69E-46 |
| MS.gene049824 | MS.gene20061 | 0.812869 | 3.41E-51 | -1.69E-46 |
| MS.gene049825 | MS.gene20061 | 0.815204 | 1.04E-51 | -1.69E-46 |
| MS.gene049887 | MS.gene20061 | 0.845606 | 3.66E-59 | -1.69E-46 |
| MS.gene050807 | MS.gene20061 | 0.82456 | 7.56E-54 | -1.69E-46 |
| MS.gene050838 | MS.gene20061 | 0.869985 | 2.05E-66 | -1.69E-46 |
| MS.gene050901 | MS.gene20061 | 0.81329 | 2.76E-51 | -1.69E-46 |
| MS.gene051247 | MS.gene20061 | 0.819946 | 8.89E-53 | -1.69E-46 |
| MS.gene051328 | MS.gene20061 | 0.802226 | 6.20E-49 | -1.69E-46 |
| MS.gene051687 | MS.gene20061 | 0.84045 | 8.63E-58 | -1.69E-46 |
| MS.gene051700 | MS.gene20061 | 0.846424 | 2.19E-59 | -1.69E-46 |
| MS.gene051719 | MS.gene20061 | 0.845365 | 4.25E-59 | -1.69E-46 |
| MS.gene051720 | MS.gene20061 | 0.839107 | 1.93E-57 | -1.69E-46 |
| MS.gene051746 | MS.gene20061 | 0.809039 | 2.30E-50 | -1.69E-46 |
| MS.gene051748 | MS.gene20061 | 0.805699 | 1.18E-49 | -1.69E-46 |
| MS.gene051850 | MS.gene20061 | 0.809909 | 1.50E-50 | -1.69E-46 |
| MS.gene051885 | MS.gene20061 | 0.837122 | 6.26E-57 | -1.69E-46 |
| MS.gene051903 | MS.gene20061 | 0.806125 | 9.57E-50 | -1.69E-46 |
| MS.gene052133 | MS.gene20061 | 0.875484 | 2.97E-68 | -1.69E-46 |
| MS.gene052335 | MS.gene20061 | 0.842009 | 3.36E-58 | -1.69E-46 |
| MS.gene052516 | MS.gene20061 | 0.81676 | 4.68E-52 | -1.69E-46 |
| MS.gene052665 | MS.gene20061 | 0.830756 | 2.45E-55 | -1.69E-46 |
| MS.gene052732 | MS.gene20061 | 0.828589 | 8.27E-55 | -1.69E-46 |
| MS.gene052879 | MS.gene20061 | 0.81196 | 5.39E-51 | -1.69E-46 |
| MS.gene052947 | MS.gene20061 | 0.856421 | 3.25E-62 | -1.69E-46 |
| MS.gene053122 | MS.gene20061 | 0.801226 | 9.95E-49 | -1.69E-46 |
| MS.gene053144 | MS.gene20061 | 0.855001 | 8.45E-62 | -1.69E-46 |
| MS.gene053171 | MS.gene20061 | 0.816438 | 5.53E-52 | -1.69E-46 |
| MS.gene053226 | MS.gene20061 | 0.805088 | 1.58E-49 | -1.69E-46 |
| MS.gene053581 | MS.gene20061 | 0.880375 | 5.78E-70 | -1.69E-46 |
| MS.gene053597 | MS.gene20061 | 0.809466 | 1.87E-50 | -1.69E-46 |
| MS.gene053599 | MS.gene20061 | 0.834191 | 3.46E-56 | -1.69E-46 |
| MS.gene05364 | MS.gene20061 | 0.814961 | 1.18E-51 | -1.69E-46 |
| MS.gene053677 | MS.gene20061 | 0.803419 | 3.52E-49 | -1.69E-46 |
| MS.gene053678 | MS.gene20061 | 0.830126 | 3.50E-55 | -1.69E-46 |
| MS.gene054250 | MS.gene20061 | 0.822941 | 1.81E-53 | -1.69E-46 |
| MS.gene054584 | MS.gene20061 | 0.847414 | 1.17E-59 | -1.69E-46 |
| MS.gene055424 | MS.gene20061 | 0.806782 | 6.96E-50 | -1.69E-46 |
| MS.gene055465 | MS.gene20061 | 0.86119 | 1.22E-63 | -1.69E-46 |
| MS.gene055466 | MS.gene20061 | 0.856644 | 2.80E-62 | -1.69E-46 |
| MS.gene055468 | MS.gene20061 | 0.880174 | 6.82E-70 | -1.69E-46 |
| MS.gene055469 | MS.gene20061 | 0.840701 | 7.42E-58 | -1.69E-46 |
| MS.gene055537 | MS.gene20061 | 0.820853 | 5.51E-53 | -1.69E-46 |
| MS.gene055555 | MS.gene20061 | 0.809411 | 1.92E-50 | -1.69E-46 |
| MS.gene055580 | MS.gene20061 | -0.862925 | 3.59E-64 | -1.69E-46 |
| MS.gene055890 | MS.gene20061 | 0.814049 | 1.88E-51 | -1.69E-46 |
| MS.gene055891 | MS.gene20061 | 0.863646 | 2.15E-64 | -1.69E-46 |
| MS.gene056043 | MS.gene20061 | -0.861567 | 9.37E-64 | -1.69E-46 |
| MS.gene056075 | MS.gene20061 | 0.831264 | 1.84E-55 | -1.69E-46 |
| MS.gene056215 | MS.gene20061 | 0.816055 | 6.74E-52 | -1.69E-46 |
| MS.gene056308 | MS.gene20061 | 0.819754 | 9.84E-53 | -1.69E-46 |
| MS.gene056432 | MS.gene20061 | 0.804591 | 2.01E-49 | -1.69E-46 |
| MS.gene056580 | MS.gene20061 | 0.805926 | 1.05E-49 | -1.69E-46 |
| MS.gene056801 | MS.gene20061 | 0.803623 | 3.19E-49 | -1.69E-46 |
| MS.gene057057 | MS.gene20061 | 0.801276 | 9.72E-49 | -1.69E-46 |
| MS.gene057102 | MS.gene20061 | 0.803545 | 3.31E-49 | -1.69E-46 |
| MS.gene057705 | MS.gene20061 | 0.83356 | 4.97E-56 | -1.69E-46 |
| MS.gene058492 | MS.gene20061 | 0.812125 | 4.96E-51 | -1.69E-46 |
| MS.gene058852 | MS.gene20061 | 0.872834 | 2.34E-67 | -1.69E-46 |
| MS.gene058906 | MS.gene20061 | 0.82729 | 1.70E-54 | -1.69E-46 |
| MS.gene058945 | MS.gene20061 | 0.810483 | 1.13E-50 | -1.69E-46 |
| MS.gene059171 | MS.gene20061 | 0.839023 | 2.03E-57 | -1.69E-46 |
| MS.gene059227 | MS.gene20061 | 0.849386 | 3.34E-60 | -1.69E-46 |
| MS.gene06001 | MS.gene20061 | 0.839764 | 1.30E-57 | -1.69E-46 |
| MS.gene060194 | MS.gene20061 | 0.810172 | 1.32E-50 | -1.69E-46 |
| MS.gene060429 | MS.gene20061 | 0.830112 | 3.53E-55 | -1.69E-46 |
| MS.gene060587 | MS.gene20061 | 0.856613 | 2.86E-62 | -1.69E-46 |
| MS.gene061100 | MS.gene20061 | 0.816663 | 4.93E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene22436 | MS.gene20061 | PPI |
| MS.gene023231 | MS.gene20061 | PPI |
| MS.gene24597 | MS.gene20061 | PPI |
| MS.gene08393 | MS.gene20061 | PPI |
| MS.gene007434 | MS.gene20061 | PPI |
| MS.gene028232 | MS.gene20061 | PPI |
| MS.gene35221 | MS.gene20061 | PPI |
| MS.gene029841 | MS.gene20061 | PPI |
| MS.gene007513 | MS.gene20061 | PPI |
| MS.gene45416 | MS.gene20061 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20061.t1 | MTR_7g101170 | 95.455 | 110 | 5 | 0 | 1 | 110 | 1 | 110 | 5.14e-72 | 209 |
| MS.gene20061.t1 | MTR_1g064540 | 81.308 | 107 | 20 | 0 | 3 | 109 | 5 | 111 | 4.43e-55 | 167 |
| MS.gene20061.t1 | MTR_7g007770 | 55.046 | 109 | 49 | 0 | 1 | 109 | 42 | 150 | 1.73e-36 | 121 |
| MS.gene20061.t1 | MTR_1g064540 | 77.500 | 80 | 18 | 0 | 30 | 109 | 10 | 89 | 3.65e-36 | 118 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20061.t1 | AT4G23710 | 69.903 | 103 | 31 | 0 | 8 | 110 | 4 | 106 | 2.20e-44 | 140 |
| MS.gene20061.t1 | AT3G01390 | 73.636 | 110 | 29 | 0 | 1 | 110 | 1 | 110 | 5.05e-42 | 134 |
| MS.gene20061.t1 | AT3G01390 | 73.636 | 110 | 29 | 0 | 1 | 110 | 1 | 110 | 5.05e-42 | 134 |
| MS.gene20061.t1 | AT3G01390 | 73.636 | 110 | 29 | 0 | 1 | 110 | 1 | 110 | 5.05e-42 | 134 |
| MS.gene20061.t1 | AT3G01390 | 73.636 | 110 | 29 | 0 | 1 | 110 | 1 | 110 | 5.05e-42 | 134 |
| MS.gene20061.t1 | AT4G25950 | 54.206 | 107 | 47 | 1 | 1 | 107 | 1 | 105 | 1.07e-33 | 113 |
| MS.gene20061.t1 | AT4G25950 | 58.462 | 65 | 27 | 0 | 1 | 65 | 1 | 65 | 3.55e-19 | 75.5 |
Find 18 sgRNAs with CRISPR-Local
Find 47 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACCGAGGCTAGTAGAATTTC+GGG | 0.229288 | 7.2:+11150773 | MS.gene20061:CDS |
| GACCGAGGCTAGTAGAATTT+CGG | 0.295846 | 7.2:+11150772 | MS.gene20061:CDS |
| CACTAAACATCGAGCTCATT+TGG | 0.305668 | 7.2:+11150551 | MS.gene20061:CDS |
| AGGTGGAATTCAACAATTAT+TGG | 0.361539 | 7.2:+11150067 | MS.gene20061:CDS |
| AATTCCACCTTGACTGTTGT+TGG | 0.367869 | 7.2:-11150054 | None:intergenic |
| ATACAGAGCAGTGGAGATTC+TGG | 0.378837 | 7.2:+11150695 | MS.gene20061:intron |
| AGCCAAAGAAGAGGCTGAAA+AGG | 0.415151 | 7.2:+11150524 | MS.gene20061:CDS |
| CCAAGAAGTCTAGTTCTTCA+CGG | 0.458780 | 7.2:-11150831 | None:intergenic |
| CCCCGAAATTCTACTAGCCT+CGG | 0.490166 | 7.2:-11150774 | None:intergenic |
| TAGCCTCGGTCTTCAGCTGA+TGG | 0.496170 | 7.2:-11150760 | None:intergenic |
| ATGGCATCCAACAACAGTCA+AGG | 0.643899 | 7.2:+11150047 | MS.gene20061:CDS |
| GATCCATCAGCTGAAGACCG+AGG | 0.646369 | 7.2:+11150757 | MS.gene20061:CDS |
| CTTCAATGAATACAGAGCAG+TGG | 0.651983 | 7.2:+11150686 | MS.gene20061:intron |
| GTTGAAACAAGCCAAAGAAG+AGG | 0.654824 | 7.2:+11150515 | MS.gene20061:CDS |
| GCATCCAACAACAGTCAAGG+TGG | 0.657348 | 7.2:+11150050 | MS.gene20061:CDS |
| CGAGGCTAGTAGAATTTCGG+GGG | 0.666069 | 7.2:+11150775 | MS.gene20061:CDS |
| CCGAGGCTAGTAGAATTTCG+GGG | 0.697212 | 7.2:+11150774 | MS.gene20061:CDS |
| CTGCTGAACAAGAAGCACAG+CGG | 0.743446 | 7.2:+11150090 | MS.gene20061:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAACAGTATAATTAAAAAG+TGG | - | chr7.2:11150392-11150411 | None:intergenic | 15.0% |
| !! | AAACAGTATAATTAAAAAGT+GGG | - | chr7.2:11150391-11150410 | None:intergenic | 15.0% |
| !! | GAGAAATAAAAAATGAAGAT+TGG | - | chr7.2:11150147-11150166 | None:intergenic | 20.0% |
| ! | AACAATCTGCTAACATAAAA+TGG | - | chr7.2:11150208-11150227 | None:intergenic | 25.0% |
| ! | AAGCTTATAATGAGATAGAA+CGG | - | chr7.2:11150657-11150676 | None:intergenic | 25.0% |
| ! | AGTATAATTAAAAAGTGGGA+GGG | - | chr7.2:11150387-11150406 | None:intergenic | 25.0% |
| ! | ATTCAGCATAATTACATAAC+TGG | - | chr7.2:11150437-11150456 | None:intergenic | 25.0% |
| ! | GTTAAAAGATAAACACAAGA+AGG | - | chr7.2:11150245-11150264 | None:intergenic | 25.0% |
| ! | TACTGTTAGAAAAATATGCT+AGG | + | chr7.2:11150493-11150512 | MS.gene20061:intron | 25.0% |
| ! | TTTGTTCTATGATATATACG+AGG | - | chr7.2:11150328-11150347 | None:intergenic | 25.0% |
| !! | TTTAACTTTCACATGAAACT+GGG | + | chr7.2:11150259-11150278 | MS.gene20061:intron | 25.0% |
| !!! | TTTTAACTTTCACATGAAAC+TGG | + | chr7.2:11150258-11150277 | MS.gene20061:intron | 25.0% |
| !!! | TTTTCAAAACAAAGTATCAG+CGG | + | chr7.2:11150581-11150600 | MS.gene20061:CDS | 25.0% |
| AGCATAATTACATAACTGGT+TGG | - | chr7.2:11150433-11150452 | None:intergenic | 30.0% | |
| AGGTGGAATTCAACAATTAT+TGG | + | chr7.2:11150067-11150086 | MS.gene20061:CDS | 30.0% | |
| ATTGTGAATGCTGCAAAAAA+TGG | + | chr7.2:11150113-11150132 | MS.gene20061:CDS | 30.0% | |
| CAGTATAATTAAAAAGTGGG+AGG | - | chr7.2:11150388-11150407 | None:intergenic | 30.0% | |
| ! | TTATCGACTGCAGTAATAAA+TGG | + | chr7.2:11150612-11150631 | MS.gene20061:intron | 30.0% |
| AATTAAAAAGTGGGAGGGAT+GGG | - | chr7.2:11150382-11150401 | None:intergenic | 35.0% | |
| TAATTAAAAAGTGGGAGGGA+TGG | - | chr7.2:11150383-11150402 | None:intergenic | 35.0% | |
| !! | ATTGGTCTTATGATGGGAAT+GGG | + | chr7.2:11150284-11150303 | MS.gene20061:intron | 35.0% |
| !! | TATTGGTCTTATGATGGGAA+TGG | + | chr7.2:11150283-11150302 | MS.gene20061:intron | 35.0% |
| AATTCCACCTTGACTGTTGT+TGG | - | chr7.2:11150057-11150076 | None:intergenic | 40.0% | |
| ATTAAAAAGTGGGAGGGATG+GGG | - | chr7.2:11150381-11150400 | None:intergenic | 40.0% | |
| CACTAAACATCGAGCTCATT+TGG | + | chr7.2:11150551-11150570 | MS.gene20061:CDS | 40.0% | |
| CTTCAATGAATACAGAGCAG+TGG | + | chr7.2:11150686-11150705 | MS.gene20061:intron | 40.0% | |
| GAAGGAAGACAGGTTTATCA+TGG | - | chr7.2:11150359-11150378 | None:intergenic | 40.0% | |
| GTTGAAACAAGCCAAAGAAG+AGG | + | chr7.2:11150515-11150534 | MS.gene20061:CDS | 40.0% | |
| TATGATATATACGAGGAGCG+AGG | - | chr7.2:11150321-11150340 | None:intergenic | 40.0% | |
| TTCACATGAAACTGGGCTAT+TGG | + | chr7.2:11150266-11150285 | MS.gene20061:intron | 40.0% | |
| !! | TGGGCTATTGGTCTTATGAT+GGG | + | chr7.2:11150278-11150297 | MS.gene20061:intron | 40.0% |
| ACCGAGGCTAGTAGAATTTC+GGG | + | chr7.2:11150773-11150792 | MS.gene20061:CDS | 45.0% | |
| AGCCAAAGAAGAGGCTGAAA+AGG | + | chr7.2:11150524-11150543 | MS.gene20061:CDS | 45.0% | |
| ATACAGAGCAGTGGAGATTC+TGG | + | chr7.2:11150695-11150714 | MS.gene20061:intron | 45.0% | |
| ATGGCATCCAACAACAGTCA+AGG | + | chr7.2:11150047-11150066 | MS.gene20061:CDS | 45.0% | |
| GACCGAGGCTAGTAGAATTT+CGG | + | chr7.2:11150772-11150791 | MS.gene20061:CDS | 45.0% | |
| ! | CTCCTTTTCAGCCTCTTCTT+TGG | - | chr7.2:11150529-11150548 | None:intergenic | 45.0% |
| !! | CTGGGCTATTGGTCTTATGA+TGG | + | chr7.2:11150277-11150296 | MS.gene20061:intron | 45.0% |
| CCGAGGCTAGTAGAATTTCG+GGG | + | chr7.2:11150774-11150793 | MS.gene20061:CDS | 50.0% | |
| CGAGGCTAGTAGAATTTCGG+GGG | + | chr7.2:11150775-11150794 | MS.gene20061:CDS | 50.0% | |
| GCATCCAACAACAGTCAAGG+TGG | + | chr7.2:11150050-11150069 | MS.gene20061:CDS | 50.0% | |
| ! | CCCCGAAATTCTACTAGCCT+CGG | - | chr7.2:11150777-11150796 | None:intergenic | 50.0% |
| ! | CTGCTGAACAAGAAGCACAG+CGG | + | chr7.2:11150090-11150109 | MS.gene20061:CDS | 50.0% |
| AAAAGTGGGAGGGATGGGGA+AGG | - | chr7.2:11150377-11150396 | None:intergenic | 55.0% | |
| GATCCATCAGCTGAAGACCG+AGG | + | chr7.2:11150757-11150776 | MS.gene20061:CDS | 55.0% | |
| TAGCCTCGGTCTTCAGCTGA+TGG | - | chr7.2:11150763-11150782 | None:intergenic | 55.0% | |
| GAGGGATGGGGAAGGAAGAC+AGG | - | chr7.2:11150369-11150388 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 11150047 | 11150844 | 11150047 | ID=MS.gene20061 |
| chr7.2 | mRNA | 11150047 | 11150844 | 11150047 | ID=MS.gene20061.t1;Parent=MS.gene20061 |
| chr7.2 | exon | 11150047 | 11150134 | 11150047 | ID=MS.gene20061.t1.exon1;Parent=MS.gene20061.t1 |
| chr7.2 | CDS | 11150047 | 11150134 | 11150047 | ID=cds.MS.gene20061.t1;Parent=MS.gene20061.t1 |
| chr7.2 | exon | 11150502 | 11150602 | 11150502 | ID=MS.gene20061.t1.exon2;Parent=MS.gene20061.t1 |
| chr7.2 | CDS | 11150502 | 11150602 | 11150502 | ID=cds.MS.gene20061.t1;Parent=MS.gene20061.t1 |
| chr7.2 | exon | 11150701 | 11150844 | 11150701 | ID=MS.gene20061.t1.exon3;Parent=MS.gene20061.t1 |
| chr7.2 | CDS | 11150701 | 11150844 | 11150701 | ID=cds.MS.gene20061.t1;Parent=MS.gene20061.t1 |
| Gene Sequence |
| Protein sequence |
