Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene007513.t1 | XP_003625625.1 | 95.5 | 110 | 5 | 0 | 1 | 110 | 1 | 110 | 1.70E-45 | 191.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene007513.t1 | O82628 | 73.6 | 110 | 29 | 0 | 1 | 110 | 1 | 110 | 1.4e-32 | 140.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene007513.t1 | Q2HV42 | 95.5 | 110 | 5 | 0 | 1 | 110 | 1 | 110 | 1.2e-45 | 191.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene35221 | MS.gene007513 | PPI |
| MS.gene029841 | MS.gene007513 | PPI |
| MS.gene007513 | MS.gene45416 | PPI |
| MS.gene22436 | MS.gene007513 | PPI |
| MS.gene023231 | MS.gene007513 | PPI |
| MS.gene007513 | MS.gene08393 | PPI |
| MS.gene24597 | MS.gene007513 | PPI |
| MS.gene007434 | MS.gene007513 | PPI |
| MS.gene007513 | MS.gene20061 | PPI |
| MS.gene028232 | MS.gene007513 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene007513.t1 | MTR_7g101170 | 95.455 | 110 | 5 | 0 | 1 | 110 | 1 | 110 | 5.14e-72 | 209 |
| MS.gene007513.t1 | MTR_1g064540 | 81.308 | 107 | 20 | 0 | 3 | 109 | 5 | 111 | 4.43e-55 | 167 |
| MS.gene007513.t1 | MTR_7g007770 | 55.046 | 109 | 49 | 0 | 1 | 109 | 42 | 150 | 1.73e-36 | 121 |
| MS.gene007513.t1 | MTR_1g064540 | 77.500 | 80 | 18 | 0 | 30 | 109 | 10 | 89 | 3.65e-36 | 118 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene007513.t1 | AT4G23710 | 69.903 | 103 | 31 | 0 | 8 | 110 | 4 | 106 | 2.20e-44 | 140 |
| MS.gene007513.t1 | AT3G01390 | 73.636 | 110 | 29 | 0 | 1 | 110 | 1 | 110 | 5.05e-42 | 134 |
| MS.gene007513.t1 | AT3G01390 | 73.636 | 110 | 29 | 0 | 1 | 110 | 1 | 110 | 5.05e-42 | 134 |
| MS.gene007513.t1 | AT3G01390 | 73.636 | 110 | 29 | 0 | 1 | 110 | 1 | 110 | 5.05e-42 | 134 |
| MS.gene007513.t1 | AT3G01390 | 73.636 | 110 | 29 | 0 | 1 | 110 | 1 | 110 | 5.05e-42 | 134 |
| MS.gene007513.t1 | AT4G25950 | 54.206 | 107 | 47 | 1 | 1 | 107 | 1 | 105 | 1.07e-33 | 113 |
| MS.gene007513.t1 | AT4G25950 | 58.462 | 65 | 27 | 0 | 1 | 65 | 1 | 65 | 3.55e-19 | 75.5 |
Find 18 sgRNAs with CRISPR-Local
Find 47 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACCGAGGCTAGTAGAATTTC+GGG | 0.229288 | 7.4:+9325807 | MS.gene007513:CDS |
| GACCGAGGCTAGTAGAATTT+CGG | 0.295846 | 7.4:+9325806 | MS.gene007513:CDS |
| CACTAAACATCGAGCTCATT+TGG | 0.305668 | 7.4:+9325585 | MS.gene007513:CDS |
| AGGTGGAATTCAACAATTAT+TGG | 0.361539 | 7.4:+9325100 | MS.gene007513:CDS |
| AATTCCACCTTGACTGTTGT+TGG | 0.367869 | 7.4:-9325087 | None:intergenic |
| ATACAGAGCAGTGGAGATTC+TGG | 0.378837 | 7.4:+9325729 | MS.gene007513:intron |
| AGCCAAAGAAGAGGCTGAAA+AGG | 0.415151 | 7.4:+9325558 | MS.gene007513:CDS |
| CCAAGAAGTCTAGTTCTTCA+CGG | 0.458780 | 7.4:-9325865 | None:intergenic |
| CCCCGAAATTCTACTAGCCT+CGG | 0.490166 | 7.4:-9325808 | None:intergenic |
| TAGCCTCGGTCTTCAGCTGA+TGG | 0.496170 | 7.4:-9325794 | None:intergenic |
| ATGGCATCCAACAACAGTCA+AGG | 0.643899 | 7.4:+9325080 | MS.gene007513:CDS |
| GATCCATCAGCTGAAGACCG+AGG | 0.646369 | 7.4:+9325791 | MS.gene007513:CDS |
| CTTCAATGAATACAGAGCAG+TGG | 0.651983 | 7.4:+9325720 | MS.gene007513:intron |
| GTTGAAACAAGCCAAAGAAG+AGG | 0.654824 | 7.4:+9325549 | MS.gene007513:CDS |
| GCATCCAACAACAGTCAAGG+TGG | 0.657348 | 7.4:+9325083 | MS.gene007513:CDS |
| CGAGGCTAGTAGAATTTCGG+GGG | 0.666069 | 7.4:+9325809 | MS.gene007513:CDS |
| CCGAGGCTAGTAGAATTTCG+GGG | 0.697212 | 7.4:+9325808 | MS.gene007513:CDS |
| CTGCTGAACAAGAAGCACAG+CGG | 0.743446 | 7.4:+9325123 | MS.gene007513:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAACAGTATAATTAAAAAG+TGG | - | chr7.4:9325425-9325444 | None:intergenic | 15.0% |
| !! | AAACAGTATAATTAAAAAGT+GGG | - | chr7.4:9325424-9325443 | None:intergenic | 15.0% |
| !! | GAGAAATAAAAAATGAAGAT+TGG | - | chr7.4:9325180-9325199 | None:intergenic | 20.0% |
| ! | AACAATCTGCTAACATAAAA+TGG | - | chr7.4:9325241-9325260 | None:intergenic | 25.0% |
| ! | AAGCTTATAATGAGATAGAA+CGG | - | chr7.4:9325691-9325710 | None:intergenic | 25.0% |
| ! | AGTATAATTAAAAAGTGGGA+GGG | - | chr7.4:9325420-9325439 | None:intergenic | 25.0% |
| ! | ATTCAGCATAATTACATAAC+TGG | - | chr7.4:9325471-9325490 | None:intergenic | 25.0% |
| ! | GTTAAAAGATAAACACAAGA+AGG | - | chr7.4:9325278-9325297 | None:intergenic | 25.0% |
| ! | TACTGTTAGAAAAATATGCT+AGG | + | chr7.4:9325527-9325546 | MS.gene007513:intron | 25.0% |
| ! | TTTGTTCTATGATATATACG+AGG | - | chr7.4:9325361-9325380 | None:intergenic | 25.0% |
| !! | TTTAACTTTCACATGAAACT+GGG | + | chr7.4:9325292-9325311 | MS.gene007513:intron | 25.0% |
| !!! | TTTTAACTTTCACATGAAAC+TGG | + | chr7.4:9325291-9325310 | MS.gene007513:intron | 25.0% |
| !!! | TTTTCAAAACAAAGTATCAG+CGG | + | chr7.4:9325615-9325634 | MS.gene007513:CDS | 25.0% |
| AGCATAATTACATAACTGGT+TGG | - | chr7.4:9325467-9325486 | None:intergenic | 30.0% | |
| AGGTGGAATTCAACAATTAT+TGG | + | chr7.4:9325100-9325119 | MS.gene007513:CDS | 30.0% | |
| ATTGTGAATGCTGCAAAAAA+TGG | + | chr7.4:9325146-9325165 | MS.gene007513:CDS | 30.0% | |
| CAGTATAATTAAAAAGTGGG+AGG | - | chr7.4:9325421-9325440 | None:intergenic | 30.0% | |
| ! | TTATCGACTGCAGTAATAAA+TGG | + | chr7.4:9325646-9325665 | MS.gene007513:intron | 30.0% |
| AATTAAAAAGTGGGAGGGAT+GGG | - | chr7.4:9325415-9325434 | None:intergenic | 35.0% | |
| TAATTAAAAAGTGGGAGGGA+TGG | - | chr7.4:9325416-9325435 | None:intergenic | 35.0% | |
| !! | ATTGGTCTTATGATGGGAAT+GGG | + | chr7.4:9325317-9325336 | MS.gene007513:intron | 35.0% |
| !! | TATTGGTCTTATGATGGGAA+TGG | + | chr7.4:9325316-9325335 | MS.gene007513:intron | 35.0% |
| AATTCCACCTTGACTGTTGT+TGG | - | chr7.4:9325090-9325109 | None:intergenic | 40.0% | |
| ATTAAAAAGTGGGAGGGATG+GGG | - | chr7.4:9325414-9325433 | None:intergenic | 40.0% | |
| CACTAAACATCGAGCTCATT+TGG | + | chr7.4:9325585-9325604 | MS.gene007513:CDS | 40.0% | |
| CTTCAATGAATACAGAGCAG+TGG | + | chr7.4:9325720-9325739 | MS.gene007513:intron | 40.0% | |
| GAAGGAAGACAGGTTTATCA+TGG | - | chr7.4:9325392-9325411 | None:intergenic | 40.0% | |
| GTTGAAACAAGCCAAAGAAG+AGG | + | chr7.4:9325549-9325568 | MS.gene007513:CDS | 40.0% | |
| TATGATATATACGAGGAGCG+AGG | - | chr7.4:9325354-9325373 | None:intergenic | 40.0% | |
| TTCACATGAAACTGGGCTAT+TGG | + | chr7.4:9325299-9325318 | MS.gene007513:intron | 40.0% | |
| !! | TGGGCTATTGGTCTTATGAT+GGG | + | chr7.4:9325311-9325330 | MS.gene007513:intron | 40.0% |
| ACCGAGGCTAGTAGAATTTC+GGG | + | chr7.4:9325807-9325826 | MS.gene007513:CDS | 45.0% | |
| AGCCAAAGAAGAGGCTGAAA+AGG | + | chr7.4:9325558-9325577 | MS.gene007513:CDS | 45.0% | |
| ATACAGAGCAGTGGAGATTC+TGG | + | chr7.4:9325729-9325748 | MS.gene007513:intron | 45.0% | |
| ATGGCATCCAACAACAGTCA+AGG | + | chr7.4:9325080-9325099 | MS.gene007513:CDS | 45.0% | |
| GACCGAGGCTAGTAGAATTT+CGG | + | chr7.4:9325806-9325825 | MS.gene007513:CDS | 45.0% | |
| ! | CTCCTTTTCAGCCTCTTCTT+TGG | - | chr7.4:9325563-9325582 | None:intergenic | 45.0% |
| !! | CTGGGCTATTGGTCTTATGA+TGG | + | chr7.4:9325310-9325329 | MS.gene007513:intron | 45.0% |
| CCGAGGCTAGTAGAATTTCG+GGG | + | chr7.4:9325808-9325827 | MS.gene007513:CDS | 50.0% | |
| CGAGGCTAGTAGAATTTCGG+GGG | + | chr7.4:9325809-9325828 | MS.gene007513:CDS | 50.0% | |
| GCATCCAACAACAGTCAAGG+TGG | + | chr7.4:9325083-9325102 | MS.gene007513:CDS | 50.0% | |
| ! | CCCCGAAATTCTACTAGCCT+CGG | - | chr7.4:9325811-9325830 | None:intergenic | 50.0% |
| ! | CTGCTGAACAAGAAGCACAG+CGG | + | chr7.4:9325123-9325142 | MS.gene007513:CDS | 50.0% |
| AAAAGTGGGAGGGATGGGGA+AGG | - | chr7.4:9325410-9325429 | None:intergenic | 55.0% | |
| GATCCATCAGCTGAAGACCG+AGG | + | chr7.4:9325791-9325810 | MS.gene007513:CDS | 55.0% | |
| TAGCCTCGGTCTTCAGCTGA+TGG | - | chr7.4:9325797-9325816 | None:intergenic | 55.0% | |
| GAGGGATGGGGAAGGAAGAC+AGG | - | chr7.4:9325402-9325421 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 9325080 | 9325878 | 9325080 | ID=MS.gene007513 |
| chr7.4 | mRNA | 9325080 | 9325878 | 9325080 | ID=MS.gene007513.t1;Parent=MS.gene007513 |
| chr7.4 | exon | 9325080 | 9325167 | 9325080 | ID=MS.gene007513.t1.exon1;Parent=MS.gene007513.t1 |
| chr7.4 | CDS | 9325080 | 9325167 | 9325080 | ID=cds.MS.gene007513.t1;Parent=MS.gene007513.t1 |
| chr7.4 | exon | 9325536 | 9325636 | 9325536 | ID=MS.gene007513.t1.exon2;Parent=MS.gene007513.t1 |
| chr7.4 | CDS | 9325536 | 9325636 | 9325536 | ID=cds.MS.gene007513.t1;Parent=MS.gene007513.t1 |
| chr7.4 | exon | 9325735 | 9325878 | 9325735 | ID=MS.gene007513.t1.exon3;Parent=MS.gene007513.t1 |
| chr7.4 | CDS | 9325735 | 9325878 | 9325735 | ID=cds.MS.gene007513.t1;Parent=MS.gene007513.t1 |
| Gene Sequence |
| Protein sequence |
