AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene64493

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene64493.t1 MTR_2g042900 96.443 253 6 1 1 250 1 253 0.0 499
MS.gene64493.t1 MTR_2g042900 85.156 256 5 2 1 223 1 256 3.06e-153 427
MS.gene64493.t1 MTR_4g124960 44.961 258 77 10 1 248 1 203 4.25e-48 158
MS.gene64493.t1 MTR_4g124960 44.934 227 69 8 1 226 1 172 5.22e-42 141
MS.gene64493.t1 MTR_5g013515 44.872 156 48 6 99 242 3 132 1.22e-34 121
MS.gene64493.t1 MTR_5g013530 44.785 163 44 5 96 242 25 157 5.26e-33 118
MS.gene64493.t1 MTR_5g013530 46.622 148 39 4 105 242 18 135 5.32e-33 117
MS.gene64493.t1 MTR_5g013520 46.897 145 42 5 105 242 11 127 1.62e-32 116
MS.gene64493.t1 MTR_8g021380 32.018 228 121 6 12 233 11 210 1.09e-22 93.2
MS.gene64493.t1 MTR_4g124950 35.583 163 58 5 69 231 1 116 5.19e-19 80.5
MS.gene64493.t1 MTR_8g021380 30.992 242 126 8 12 223 11 241 3.39e-15 73.6
MS.gene64493.t1 MTR_2g044910 32.031 128 62 5 109 230 15 123 1.86e-13 66.6
MS.gene64493.t1 MTR_2g044910 32.031 128 62 5 109 230 58 166 2.73e-13 67.0
MS.gene64493.t1 MTR_1g031930 30.460 174 67 4 107 237 111 273 1.90e-12 66.2
MS.gene64493.t1 MTR_1g031930 30.460 174 67 4 107 237 172 334 9.20e-12 64.3
MS.gene64493.t1 MTR_4g124360 28.682 129 73 3 105 230 3 115 9.96e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene64493.t1 AT1G74950 36.525 282 100 10 2 242 3 246 1.77e-42 145
MS.gene64493.t1 AT1G19180 37.037 270 119 8 1 242 1 247 3.75e-41 142
MS.gene64493.t1 AT1G19180 37.037 270 119 8 1 242 1 247 3.75e-41 142
MS.gene64493.t1 AT1G74950 36.187 257 91 9 2 223 3 221 3.31e-36 128
MS.gene64493.t1 AT1G72450 32.328 232 121 7 17 234 4 213 4.79e-27 105
MS.gene64493.t1 AT1G17380 31.076 251 128 7 1 241 1 216 1.38e-25 101
MS.gene64493.t1 AT1G19180 39.041 146 59 4 106 242 57 181 2.02e-22 91.3
MS.gene64493.t1 AT1G72450 30.317 221 118 10 17 223 4 202 3.93e-22 90.9
MS.gene64493.t1 AT1G17380 30.043 233 118 7 1 223 1 198 4.29e-20 85.9
MS.gene64493.t1 AT1G17380 35.211 142 58 5 105 236 65 182 1.16e-14 71.6
MS.gene64493.t1 AT5G20900 32.787 122 67 2 109 230 57 163 1.38e-12 64.7
MS.gene64493.t1 AT3G17860 32.278 158 72 5 108 234 144 297 5.25e-11 62.0
MS.gene64493.t1 AT3G17860 32.278 158 72 5 108 234 144 297 5.25e-11 62.0
MS.gene64493.t1 AT3G17860 32.278 158 72 5 108 234 177 330 5.29e-11 62.4
MS.gene64493.t1 AT3G17860 32.278 158 72 5 108 234 164 317 7.65e-11 61.6

Find 59 sgRNAs with CRISPR-Local

Find 123 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTATTGAGTCAATATATTA+AGG 0.231958 2.4:-47334750 MS.gene64493:CDS
ACAGAAGTTCTGACCAAATT+AGG 0.245326 2.4:+47333279 None:intergenic
GCCTTCATTTCCTCCTAATT+TGG 0.255924 2.4:-47333292 MS.gene64493:CDS
AGCCTCGCTTCATCGGTTTC+TGG 0.260318 2.4:-47333037 MS.gene64493:CDS
GCTGGCTTATTAACTTGATA+TGG 0.264533 2.4:+47332856 None:intergenic
CATGAGCTCATGTGCTTTAT+CGG 0.271727 2.4:+47333380 None:intergenic
AGCGAGGCTTTCCTCATAAT+TGG 0.283392 2.4:+47333051 None:intergenic
TCGGAATATTCAGAAGTTTC+CGG 0.324209 2.4:-47334827 MS.gene64493:CDS
ATTGTCAATTGTGCAGCTTT+AGG 0.328266 2.4:+47333444 None:intergenic
CTTCGCCGGTGGTTTGTTGC+CGG 0.328284 2.4:+47334808 None:intergenic
ACAAAAGAACCTTACAACTA+TGG 0.341905 2.4:-47334053 MS.gene64493:CDS
GAGTCAATATATTAAGGAAA+AGG 0.376605 2.4:-47334744 MS.gene64493:CDS
CCGGTGCCGGTGGCAGTACT+AGG 0.378749 2.4:+47333216 None:intergenic
TGCTTCAAAGATCTTTCTCT+TGG 0.389248 2.4:-47334719 MS.gene64493:CDS
CATGGTTGGAAACAAGTTCA+TGG 0.390560 2.4:+47334090 None:intergenic
AAGAAATTACCGCAAAGATT+AGG 0.403918 2.4:+47333159 None:intergenic
AGTCAATATATTAAGGAAAA+GGG 0.413916 2.4:-47334743 MS.gene64493:CDS
AATGATTTGCAGTGCTATCA+AGG 0.444008 2.4:-47333469 MS.gene64493:intron
CCTTCCAGACCTAATCTTTG+CGG 0.448657 2.4:-47333168 MS.gene64493:intron
TTCATTAATCGAGCCGGTGC+CGG 0.460250 2.4:+47333203 None:intergenic
ATTACCGCAAAGATTAGGTC+TGG 0.470927 2.4:+47333164 None:intergenic
AGCTCATGTGCTTTATCGGC+AGG 0.475762 2.4:+47333384 None:intergenic
GAGTCCATGTCATGGCTTGT+GGG 0.483230 2.4:-47332832 MS.gene64493:CDS
GCGCAGATCTGCCAATTATG+AGG 0.500927 2.4:-47333062 MS.gene64493:intron
TAGGTCTGGAAGGCACTTGA+AGG 0.506440 2.4:+47333178 None:intergenic
ACATGCAACACAGACCCTTC+TGG 0.507485 2.4:-47334692 MS.gene64493:intron
TGAGGAAAGCCTCGCTTCAT+CGG 0.508146 2.4:-47333044 MS.gene64493:CDS
ATGTTCACGGTAGGAACAAT+CGG 0.508733 2.4:+47333243 None:intergenic
TCGGTTTCTGGAGAAGAGAA+AGG 0.512539 2.4:-47333025 MS.gene64493:CDS
CAAACCACCGGCGAAGTCAC+CGG 0.513459 2.4:-47334801 MS.gene64493:CDS
CATGAACTTGTTTCCAACCA+TGG 0.520010 2.4:-47334089 MS.gene64493:CDS
CGGTGCCGGTGGCAGTACTA+GGG 0.531615 2.4:+47333217 None:intergenic
CGATAAAGCACATGAGCTCA+TGG 0.531706 2.4:-47333379 MS.gene64493:CDS
TGAGCAAATCCATAGTTGTA+AGG 0.532508 2.4:+47334044 None:intergenic
ATTAACTTGATATGGTGCAT+TGG 0.537946 2.4:+47332864 None:intergenic
TGAGTCCATGTCATGGCTTG+TGG 0.539136 2.4:-47332833 MS.gene64493:CDS
GAATTGTTCAAAGCAGCTTG+TGG 0.542652 2.4:+47334019 None:intergenic
CCTAGTACTGCCACCGGCAC+CGG 0.542742 2.4:-47333216 MS.gene64493:CDS
TTCAACTATGTCTACCTCAT+CGG 0.558400 2.4:-47334846 None:intergenic
TGCACCCACAAGCCATGACA+TGG 0.562173 2.4:+47332828 None:intergenic
ACCAAATTAGGAGGAAATGA+AGG 0.565826 2.4:+47333291 None:intergenic
TTCTCCGGTGACTTCGCCGG+TGG 0.568647 2.4:+47334797 None:intergenic
AAGGTGTTCATTAATCGAGC+CGG 0.572295 2.4:+47333197 None:intergenic
AGCCATGACATGGACTCAGC+TGG 0.572954 2.4:+47332838 None:intergenic
AATTAGGAGGAAATGAAGGC+TGG 0.580229 2.4:+47333295 None:intergenic
ATTAATCGAGCCGGTGCCGG+TGG 0.588264 2.4:+47333206 None:intergenic
CCGCAAAGATTAGGTCTGGA+AGG 0.596635 2.4:+47333168 None:intergenic
ATCATCCCTAGTACTGCCAC+CGG 0.601610 2.4:-47333222 MS.gene64493:CDS
GAAGTTCTGACCAAATTAGG+AGG 0.613944 2.4:+47333282 None:intergenic
CTCCAGAAACCGATGAAGCG+AGG 0.614975 2.4:+47333035 None:intergenic
AGTACTAGGGATGATGTTCA+CGG 0.617551 2.4:+47333230 None:intergenic
TTCTGGAGAAGAGAAAGGAT+AGG 0.619484 2.4:-47333020 MS.gene64493:intron
ATGATTTGCAGTGCTATCAA+GGG 0.622638 2.4:-47333468 MS.gene64493:intron
AGTTTCCGGCAACAAACCAC+CGG 0.625545 2.4:-47334813 MS.gene64493:CDS
ATTAAGGTTCCGTACCAGAA+GGG 0.632538 2.4:+47334678 None:intergenic
AGCCAGCTGAGTCCATGTCA+TGG 0.640187 2.4:-47332840 MS.gene64493:CDS
CTAGGGATGATGTTCACGGT+AGG 0.671499 2.4:+47333234 None:intergenic
AACTTCTGAATATTCCGATG+AGG 0.677602 2.4:+47334832 None:intergenic
CACGGTAGGAACAATCGGAG+TGG 0.687305 2.4:+47333248 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTATTATTTTTTAAAATT+TGG - chr2.4:47333083-47333102 MS.gene64493:intron 0.0%
!! AAAAATTAATAATCAACTAA+TGG + chr2.4:47334122-47334141 None:intergenic 10.0%
!! AGTTAAAAAAATAAATTTGA+TGG - chr2.4:47333860-47333879 MS.gene64493:intron 10.0%
!!! ATTGTTATTATATACTAATT+AGG - chr2.4:47333380-47333399 MS.gene64493:CDS 10.0%
!!! ATTTGTATTTTTTTTTTTGT+AGG - chr2.4:47333504-47333523 MS.gene64493:intron 10.0%
!!! TTTGTATTTTTTTTTTTGTA+GGG - chr2.4:47333505-47333524 MS.gene64493:intron 10.0%
!! CAAAATAAAAATAATCAACA+TGG + chr2.4:47333295-47333314 None:intergenic 15.0%
!! TGCAATAGAATAATTTAAAA+TGG + chr2.4:47333249-47333268 None:intergenic 15.0%
!! TTTATTGAGTCAATATATTA+AGG - chr2.4:47332899-47332918 MS.gene64493:intron 15.0%
!!! AACTTTTGAAATTTTTTTGT+GGG - chr2.4:47333053-47333072 MS.gene64493:CDS 15.0%
!!! ATTTTTTTACAAACCTAATT+TGG + chr2.4:47333706-47333725 None:intergenic 15.0%
!!! TTTAAAATTTGGTATCTTAA+TGG - chr2.4:47333094-47333113 MS.gene64493:intron 15.0%
!!! TTTTTTTTTCCTTTTTTGTA+AGG - chr2.4:47333193-47333212 MS.gene64493:CDS 15.0%
!! AAAAAATAAATTTGATGGAG+AGG - chr2.4:47333865-47333884 MS.gene64493:intron 20.0%
!! AGTCAATATATTAAGGAAAA+GGG - chr2.4:47332906-47332925 MS.gene64493:intron 20.0%
!! ATTTCATTTCATACCAAATT+AGG - chr2.4:47333690-47333709 MS.gene64493:intron 20.0%
!! GAAACAAAAAACGAAAAATT+CGG + chr2.4:47333001-47333020 None:intergenic 20.0%
!!! AATTTTTCGTTTTTTGTTTC+TGG - chr2.4:47333001-47333020 MS.gene64493:intron 20.0%
!!! ATTTTTCGTTTTTTGTTTCT+GGG - chr2.4:47333002-47333021 MS.gene64493:intron 20.0%
!!! CAACTTTTGAAATTTTTTTG+TGG - chr2.4:47333052-47333071 MS.gene64493:CDS 20.0%
!!! CAATTGACAATGTTTTATAA+CGG - chr2.4:47334217-47334236 MS.gene64493:intron 20.0%
! AACCATTACCACTTAAAATT+TGG - chr2.4:47334515-47334534 MS.gene64493:intron 25.0%
! AATTTATGAGTGATGACAAA+TGG - chr2.4:47333727-47333746 MS.gene64493:intron 25.0%
! ATTATTCCTTTCTTCTTAGT+TGG - chr2.4:47333120-47333139 MS.gene64493:intron 25.0%
! ATTCTAGTACTTAAGAGTAA+CGG - chr2.4:47333157-47333176 MS.gene64493:intron 25.0%
! GAGTCAATATATTAAGGAAA+AGG - chr2.4:47332905-47332924 MS.gene64493:intron 25.0%
!! ATAGTTCTACCTTACAAAAA+AGG + chr2.4:47333205-47333224 None:intergenic 25.0%
!!! ATCCAAATTTTAAGTGGTAA+TGG + chr2.4:47334520-47334539 None:intergenic 25.0%
AAGAAATTACCGCAAAGATT+AGG + chr2.4:47334493-47334512 None:intergenic 30.0%
ACAAAAGAACCTTACAACTA+TGG - chr2.4:47333596-47333615 MS.gene64493:intron 30.0%
ATTTGGATGACACGAAAAAT+TGG - chr2.4:47334532-47334551 MS.gene64493:intron 30.0%
CAAAATGAAGAAAACTCACT+TGG + chr2.4:47333655-47333674 None:intergenic 30.0%
CGAAAAATTCGGAGAAATTA+AGG + chr2.4:47332990-47333009 None:intergenic 30.0%
TATGAACCAACTAAGAAGAA+AGG + chr2.4:47333129-47333148 None:intergenic 30.0%
TGCTATCAATCATTACTAAG+CGG + chr2.4:47334045-47334064 None:intergenic 30.0%
! ATTAACTTGATATGGTGCAT+TGG + chr2.4:47334788-47334807 None:intergenic 30.0%
! TTGTGTCAAATTGTTTTCCA+TGG + chr2.4:47333580-47333599 None:intergenic 30.0%
!! TTTTTAACTAGTTCCACCTA+GGG + chr2.4:47333849-47333868 None:intergenic 30.0%
!!! TTTTTTAACTAGTTCCACCT+AGG + chr2.4:47333850-47333869 None:intergenic 30.0%
AACTTCTGAATATTCCGATG+AGG + chr2.4:47332820-47332839 None:intergenic 35.0%
ACCAAATTAGGAGGAAATGA+AGG + chr2.4:47334361-47334380 None:intergenic 35.0%
ATTGTCAATTGTGCAGCTTT+AGG + chr2.4:47334208-47334227 None:intergenic 35.0%
TATCCTCTACAATTGCAGTA+TGG + chr2.4:47334004-47334023 None:intergenic 35.0%
TCGGAATATTCAGAAGTTTC+CGG - chr2.4:47332822-47332841 MS.gene64493:CDS 35.0%
TGAGCAAATCCATAGTTGTA+AGG + chr2.4:47333608-47333627 None:intergenic 35.0%
TGCTTCAAAGATCTTTCTCT+TGG - chr2.4:47332930-47332949 MS.gene64493:intron 35.0%
! AATGATTTGCAGTGCTATCA+AGG - chr2.4:47334180-47334199 MS.gene64493:intron 35.0%
! ACAGAAGTTCTGACCAAATT+AGG + chr2.4:47334373-47334392 None:intergenic 35.0%
! ATGATTTGCAGTGCTATCAA+GGG - chr2.4:47334181-47334200 MS.gene64493:intron 35.0%
! CGTGTCATCCAAATTTTAAG+TGG + chr2.4:47334526-47334545 None:intergenic 35.0%
! GCTGGCTTATTAACTTGATA+TGG + chr2.4:47334796-47334815 None:intergenic 35.0%
! GTCAAATTGTTTTCCATGGT+TGG + chr2.4:47333576-47333595 None:intergenic 35.0%
!! TGAGAGAAAGTTGTTTTCTC+CGG + chr2.4:47332870-47332889 None:intergenic 35.0%
!!! CGTTTTTTGTTTCTGGGTTT+TGG - chr2.4:47333008-47333027 MS.gene64493:intron 35.0%
AATTAAGGTTCCGTACCAGA+AGG + chr2.4:47332975-47332994 None:intergenic 40.0%
AATTAGGAGGAAATGAAGGC+TGG + chr2.4:47334357-47334376 None:intergenic 40.0%
ACCTAGATCCTCTACAGATA+CGG - chr2.4:47333895-47333914 MS.gene64493:intron 40.0%
AGTACTAGGGATGATGTTCA+CGG + chr2.4:47334422-47334441 None:intergenic 40.0%
ATCAATCATTACTAAGCGGC+TGG + chr2.4:47334041-47334060 None:intergenic 40.0%
ATGTTCACGGTAGGAACAAT+CGG + chr2.4:47334409-47334428 None:intergenic 40.0%
ATTAAGGTTCCGTACCAGAA+GGG + chr2.4:47332974-47332993 None:intergenic 40.0%
ATTACCGCAAAGATTAGGTC+TGG + chr2.4:47334488-47334507 None:intergenic 40.0%
CATGAGCTCATGTGCTTTAT+CGG + chr2.4:47334272-47334291 None:intergenic 40.0%
CATGGTTGGAAACAAGTTCA+TGG + chr2.4:47333562-47333581 None:intergenic 40.0%
GAATTGTTCAAAGCAGCTTG+TGG + chr2.4:47333633-47333652 None:intergenic 40.0%
GCCTTCATTTCCTCCTAATT+TGG - chr2.4:47334357-47334376 MS.gene64493:intron 40.0%
TTTCCACGAATTGACCCTTT+CGG + chr2.4:47333800-47333819 None:intergenic 40.0%
! AAGGTGTTCATTAATCGAGC+CGG + chr2.4:47334455-47334474 None:intergenic 40.0%
! CATGAACTTGTTTCCAACCA+TGG - chr2.4:47333560-47333579 MS.gene64493:intron 40.0%
! CTCATGGCTTTTGCTAACAA+AGG - chr2.4:47334286-47334305 MS.gene64493:intron 40.0%
! GAAGTTCTGACCAAATTAGG+AGG + chr2.4:47334370-47334389 None:intergenic 40.0%
! TTCGTGGAAATAACTTGCAG+AGG - chr2.4:47333810-47333829 MS.gene64493:intron 40.0%
! TTCTGGAGAAGAGAAAGGAT+AGG - chr2.4:47334629-47334648 MS.gene64493:intron 40.0%
ACCGTATCTGTAGAGGATCT+AGG + chr2.4:47333899-47333918 None:intergenic 45.0%
AGCGAGGCTTTCCTCATAAT+TGG + chr2.4:47334601-47334620 None:intergenic 45.0%
AGGATTTAGATCCTCTCCCT+AGG - chr2.4:47333830-47333849 MS.gene64493:intron 45.0%
ATCTGTAGAGGATCTAGGTG+TGG + chr2.4:47333894-47333913 None:intergenic 45.0%
ATTGGCAGATCTGCGCATAA+AGG + chr2.4:47334583-47334602 None:intergenic 45.0%
ATTTAGATCCTCTCCCTAGG+TGG - chr2.4:47333833-47333852 MS.gene64493:intron 45.0%
CCTTCCAGACCTAATCTTTG+CGG - chr2.4:47334481-47334500 MS.gene64493:intron 45.0%
CGATAAAGCACATGAGCTCA+TGG - chr2.4:47334270-47334289 MS.gene64493:intron 45.0%
GATCCTCTACAGATACGGTA+TGG - chr2.4:47333900-47333919 MS.gene64493:intron 45.0%
GCACCATACTGCAATTGTAG+AGG - chr2.4:47333998-47334017 MS.gene64493:intron 45.0%
GGAGAAGAGAAAGGATAGGT+AGG - chr2.4:47334633-47334652 MS.gene64493:intron 45.0%
TCTGTAGAGGATCTAGGTGT+GGG + chr2.4:47333893-47333912 None:intergenic 45.0%
! TCGGTTTCTGGAGAAGAGAA+AGG - chr2.4:47334624-47334643 MS.gene64493:intron 45.0%
AACTAGTTCCACCTAGGGAG+AGG + chr2.4:47333844-47333863 None:intergenic 50.0%
ACATGCAACACAGACCCTTC+TGG - chr2.4:47332957-47332976 MS.gene64493:intron 50.0%
ACGAATTGACCCTTTCGGCA+AGG + chr2.4:47333795-47333814 None:intergenic 50.0%
AGCTCATGTGCTTTATCGGC+AGG + chr2.4:47334268-47334287 None:intergenic 50.0%
AGTTTCCGGCAACAAACCAC+CGG - chr2.4:47332836-47332855 MS.gene64493:CDS 50.0%
ATCATCCCTAGTACTGCCAC+CGG - chr2.4:47334427-47334446 MS.gene64493:intron 50.0%
CAACACAGACCCTTCTGGTA+CGG - chr2.4:47332962-47332981 MS.gene64493:intron 50.0%
CCGCAAAGATTAGGTCTGGA+AGG + chr2.4:47334484-47334503 None:intergenic 50.0%
CTAGGGATGATGTTCACGGT+AGG + chr2.4:47334418-47334437 None:intergenic 50.0%
GCACCATACCGTATCTGTAG+AGG + chr2.4:47333906-47333925 None:intergenic 50.0%
GCGCAGATCTGCCAATTATG+AGG - chr2.4:47334587-47334606 MS.gene64493:intron 50.0%
TATCGTCACGCTAGCCGATA+AGG + chr2.4:47333763-47333782 None:intergenic 50.0%
TGAGGAAAGCCTCGCTTCAT+CGG - chr2.4:47334605-47334624 MS.gene64493:intron 50.0%
TGAGTCCATGTCATGGCTTG+TGG - chr2.4:47334816-47334835 MS.gene64493:CDS 50.0%
TTCATTAATCGAGCCGGTGC+CGG + chr2.4:47334449-47334468 None:intergenic 50.0%
TTGCCGAAAGGGTCAATTCG+TGG - chr2.4:47333794-47333813 MS.gene64493:intron 50.0%
! GAGTCCATGTCATGGCTTGT+GGG - chr2.4:47334817-47334836 MS.gene64493:CDS 50.0%
! TCATTACTAAGCGGCTGGCT+CGG + chr2.4:47334036-47334055 None:intergenic 50.0%
!! TAGGTCTGGAAGGCACTTGA+AGG + chr2.4:47334474-47334493 None:intergenic 50.0%
AGCCAGCTGAGTCCATGTCA+TGG - chr2.4:47334809-47334828 MS.gene64493:CDS 55.0%
AGCCATGACATGGACTCAGC+TGG + chr2.4:47334814-47334833 None:intergenic 55.0%
ATAGCTGTCGCCTTGCCGAA+AGG - chr2.4:47333782-47333801 MS.gene64493:intron 55.0%
CACGGTAGGAACAATCGGAG+TGG + chr2.4:47334404-47334423 None:intergenic 55.0%
CTCCAGAAACCGATGAAGCG+AGG + chr2.4:47334617-47334636 None:intergenic 55.0%
TAGCTGTCGCCTTGCCGAAA+GGG - chr2.4:47333783-47333802 MS.gene64493:intron 55.0%
TGCACCCACAAGCCATGACA+TGG + chr2.4:47334824-47334843 None:intergenic 55.0%
! ATGGCGAGAGCGAGCCTTAT+CGG - chr2.4:47333746-47333765 MS.gene64493:intron 55.0%
! GTTTTCTCCGGTGACTTCGC+CGG + chr2.4:47332858-47332877 None:intergenic 55.0%
!! AGCCTCGCTTCATCGGTTTC+TGG - chr2.4:47334612-47334631 MS.gene64493:intron 55.0%
CAAACCACCGGCGAAGTCAC+CGG - chr2.4:47332848-47332867 MS.gene64493:CDS 60.0%
! ATTAATCGAGCCGGTGCCGG+TGG + chr2.4:47334446-47334465 None:intergenic 60.0%
!! CTTCGCCGGTGGTTTGTTGC+CGG + chr2.4:47332844-47332863 None:intergenic 60.0%
!! TGGTGCTGCAGCTGTAGCGA+CGG + chr2.4:47333984-47334003 None:intergenic 60.0%
CCTAGTACTGCCACCGGCAC+CGG - chr2.4:47334433-47334452 MS.gene64493:intron 65.0%
TTCTCCGGTGACTTCGCCGG+TGG + chr2.4:47332855-47332874 None:intergenic 65.0%
!! CGGTGCCGGTGGCAGTACTA+GGG + chr2.4:47334435-47334454 None:intergenic 65.0%
!! CCGGTGCCGGTGGCAGTACT+AGG + chr2.4:47334436-47334455 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 47332810 47334861 47332810 ID=MS.gene64493
chr2.4 mRNA 47332810 47334861 47332810 ID=MS.gene64493.t1;Parent=MS.gene64493
chr2.4 exon 47334693 47334861 47334693 ID=MS.gene64493.t1.exon1;Parent=MS.gene64493.t1
chr2.4 CDS 47334693 47334861 47334693 ID=cds.MS.gene64493.t1;Parent=MS.gene64493.t1
chr2.4 exon 47334016 47334145 47334016 ID=MS.gene64493.t1.exon2;Parent=MS.gene64493.t1
chr2.4 CDS 47334016 47334145 47334016 ID=cds.MS.gene64493.t1;Parent=MS.gene64493.t1
chr2.4 exon 47333169 47333479 47333169 ID=MS.gene64493.t1.exon3;Parent=MS.gene64493.t1
chr2.4 CDS 47333169 47333479 47333169 ID=cds.MS.gene64493.t1;Parent=MS.gene64493.t1
chr2.4 exon 47333021 47333078 47333021 ID=MS.gene64493.t1.exon4;Parent=MS.gene64493.t1
chr2.4 CDS 47333021 47333078 47333021 ID=cds.MS.gene64493.t1;Parent=MS.gene64493.t1
chr2.4 exon 47332810 47332894 47332810 ID=MS.gene64493.t1.exon5;Parent=MS.gene64493.t1
chr2.4 CDS 47332810 47332894 47332810 ID=cds.MS.gene64493.t1;Parent=MS.gene64493.t1
Gene Sequence

>MS.gene64493

ATGTCTACCTCATCGGAATATTCAGAAGTTTCCGGCAACAAACCACCGGCGAAGTCACCGGAGAAAACAACTTTCTCTCAAACATGTAGTTTATTGAGTCAATATATTAAGGAAAAGGGTTGCTTCAAAGATCTTTCTCTTGGTATCACATGCAACACAGACCCTTCTGGTACGGAACCTTAATTTCTCCGAATTTTTCGTTTTTTGTTTCTGGGTTTTGGTTTTTTTTTTTTTTTTTTGTTCAACTTTTGAAATTTTTTTGTGGGTGTTATTTATTATTATTTTTTAAAATTTGGTATCTTAATGGTTAATTATTCCTTTCTTCTTAGTTGGTTCATAATTATTAAATTCTAGTACTTAAGAGTAACGGTTAATTTTGTTAATTTTTTTTTCCTTTTTTGTAAGGTAGAACTATATCTAAATTTTCAAATATTTGCCATTTTAAATTATTCTATTGCATGTTGTTTATTTTTATTTTGAATCCATGTTGATTATTTTTATTTTGAGACTTTTAAAATTCATAGATCTAATTTATAGCTCTAATTAATAGATAATTTGTTCATAATTCTAATTGTTATTATATACTAATTAGGTTGTTAAATTTGATAAGATTGTTATCTTGTTGTATAATTTTATTTGTTTTGTTAATTTTGTTAAATTTTTTTTTTTGTGTAATATTGTTTGTTTTTTCTAAATTTGTATTTTTTTTTTTGTAGGGTCTTCTGAGACTTCTTCTCAATCTGCAACAACCATGAACTTGTTTCCAACCATGGAAAACAATTTGACACAAAAGAACCTTACAACTATGGATTTGCTCACTCCACAAGCTGCTTTGAACAATTCCAAGTGAGTTTTCTTCATTTTGTTCTGATTTCAATTAATTTCATTTCATACCAAATTAGGTTTGTAAAAAAATTAATTTATGAGTGATGACAAATGGCGAGAGCGAGCCTTATCGGCTAGCGTGACGATATAGCTGTCGCCTTGCCGAAAGGGTCAATTCGTGGAAATAACTTGCAGAGGATTTAGATCCTCTCCCTAGGTGGAACTAGTTAAAAAAATAAATTTGATGGAGAGGATCCCACACCTAGATCCTCTACAGATACGGTATGGTGCTACATCTGTAAGAACGTTATATTTAGAGAGATCAAATTTCACTCGCTAAATGTAACCGTCGCTACAGCTGCAGCACCATACTGCAATTGTAGAGGATAAGTTGTGAACCGAGCCAGCCGCTTAGTAATGATTGATAGCAATTTATAAATGTTGTGCTGATTTAATAATCTTGATATAGTTCGAGATATCAAAACCATTAGTTGATTATTAATTTTTTTTTTCTTCGATTAGAGACTAATATATGATGTTGTATGAATGATTTGCAGTGCTATCAAGGGACCTAAAGCTGCACAATTGACAATGTTTTATAACGGTCAAGTTATTGTATTCGATGATTTTCCTGCCGATAAAGCACATGAGCTCATGGCTTTTGCTAACAAAGGAATCTCTCAAAGTCAGAACTATTCTGTGTACACTTACACACAGAGCCAGCCTTCATTTCCTCCTAATTTGGTCAGAACTTCTGTTAACACAGCCACTCCGATTGTTCCTACCGTGAACATCATCCCTAGTACTGCCACCGGCACCGGCTCGATTAATGAACACCTTCAAGTGCCTTCCAGACCTAATCTTTGCGGTAATTTCTTGAAACCATTACCACTTAAAATTTGGATGACACGAAAAATTGGATCATGTTTTTCTGATCATGAATTTCCTTTATGCGCAGATCTGCCAATTATGAGGAAAGCCTCGCTTCATCGGTTTCTGGAGAAGAGAAAGGATAGGTAGGCATTCTCGATTAAATTAAAACTTGTAAATGAACTATTTTTTACATCTTTTCTAACTTAGCTTGTTGATCAAGTATTTTTGCTGAGATAATAATAATTTGTTTCATTGTTGTGTATTTACAGAATTGCTGCCAATGCACCATATCAAGTTAATAAGCCAGCTGAGTCCATGTCATGGCTTGTGGGTGCAAAATCAACTCAAATTTGA
Protein sequence

>MS.gene64493.t1

MSTSSEYSEVSGNKPPAKSPEKTTFSQTCSLLSQYIKEKGCFKDLSLGITCNTDPSGSSETSSQSATTMNLFPTMENNLTQKNLTTMDLLTPQAALNNSNAIKGPKAAQLTMFYNGQVIVFDDFPADKAHELMAFANKGISQSQNYSVYTYTQSQPSFPPNLVRTSVNTATPIVPTVNIIPSTATGTGSINEHLQVPSRPNLCDLPIMRKASLHRFLEKRKDRIAANAPYQVNKPAESMSWLVGAKSTQI