AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene027737

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene027737.t1 MTR_5g032780 99.214 636 5 0 1 636 1 636 0.0 1300
MS.gene027737.t1 MTR_8g069330 72.144 639 132 10 1 636 1 596 0.0 869
MS.gene027737.t1 MTR_4g134210 48.467 522 223 7 1 521 1 477 1.90e-162 479
MS.gene027737.t1 MTR_5g015790 46.429 560 250 9 1 551 1 519 2.21e-159 472
MS.gene027737.t1 MTR_1g014070 46.869 527 231 10 1 518 1 487 1.84e-152 454
MS.gene027737.t1 MTR_1g106430 42.989 542 269 7 1 537 1 507 5.50e-147 440
MS.gene027737.t1 MTR_2g027410 40.758 633 284 12 1 610 1 565 4.09e-146 436
MS.gene027737.t1 MTR_4g093850 39.744 546 279 8 5 523 23 545 8.58e-128 392
MS.gene027737.t1 MTR_2g009340 40.145 553 274 9 5 523 23 552 2.43e-127 391
MS.gene027737.t1 MTR_2g027410 39.535 559 249 11 75 610 1 493 6.66e-119 363
MS.gene027737.t1 MTR_2g018750 40.414 532 278 9 1 523 1 502 2.31e-115 358
MS.gene027737.t1 MTR_1g064360 38.432 523 282 7 1 523 1 483 1.10e-112 349
MS.gene027737.t1 MTR_1g094850 39.400 533 278 11 1 523 1 498 6.31e-112 349
MS.gene027737.t1 MTR_5g016060 37.430 537 240 10 22 529 36 505 1.34e-109 343
MS.gene027737.t1 MTR_8g090220 35.962 520 259 7 16 515 42 507 1.42e-108 337
MS.gene027737.t1 MTR_8g090220 34.964 552 274 8 16 536 42 539 6.32e-108 339
MS.gene027737.t1 MTR_8g078900 38.224 518 265 9 28 517 22 512 1.51e-106 336
MS.gene027737.t1 MTR_2g084595 35.751 579 326 10 24 591 21 564 2.44e-106 335
MS.gene027737.t1 MTR_8g078900 38.462 507 257 9 39 517 3 482 3.36e-104 329
MS.gene027737.t1 MTR_7g073980 38.124 501 267 9 22 519 29 489 4.81e-102 324
MS.gene027737.t1 MTR_8g078900 39.956 458 238 7 70 517 42 472 4.85e-102 323
MS.gene027737.t1 MTR_4g022330 36.412 563 295 10 22 581 30 532 8.36e-98 312
MS.gene027737.t1 MTR_4g093850 36.049 491 264 7 5 468 23 490 2.43e-96 306
MS.gene027737.t1 MTR_1g088885 37.288 531 271 14 12 525 18 503 1.75e-93 301
MS.gene027737.t1 MTR_4g095430 36.400 500 271 7 23 519 10 465 2.03e-93 300
MS.gene027737.t1 MTR_2g048390 37.214 481 260 9 1 468 1 452 2.14e-90 288
MS.gene027737.t1 MTR_7g108290 35.884 588 314 14 12 584 18 557 3.09e-90 294
MS.gene027737.t1 MTR_3g062540 34.449 508 274 8 21 526 19 469 8.76e-87 282
MS.gene027737.t1 MTR_2g084595 36.062 452 257 8 24 469 21 446 8.53e-83 268
MS.gene027737.t1 MTR_5g016060 35.683 454 198 9 105 529 3 391 8.01e-82 267
MS.gene027737.t1 MTR_8g078900 34.177 474 257 8 23 468 17 463 1.91e-80 262
MS.gene027737.t1 MTR_3g062540 33.954 483 260 8 46 526 2 427 2.57e-78 258
MS.gene027737.t1 MTR_5g075640 33.861 505 282 8 22 524 21 475 4.43e-77 256
MS.gene027737.t1 MTR_8g078900 35.941 409 225 6 70 468 42 423 9.30e-76 248
MS.gene027737.t1 MTR_1g082690 31.452 496 262 10 27 518 59 480 4.13e-72 243
MS.gene027737.t1 MTR_5g075640 33.264 481 269 7 46 524 2 432 5.98e-70 236
MS.gene027737.t1 MTR_4g011530 38.188 309 150 4 211 519 46 313 1.35e-65 222
MS.gene027737.t1 MTR_2g102730 39.159 309 149 4 211 519 24 293 2.02e-62 213
MS.gene027737.t1 MTR_4g045767 29.075 454 251 13 74 519 52 442 5.06e-47 174
MS.gene027737.t1 MTR_4g074190 27.255 521 301 12 27 523 4 470 1.86e-45 170
MS.gene027737.t1 MTR_4g074190 27.521 476 269 10 70 523 3 424 1.23e-43 164
MS.gene027737.t1 MTR_1g035720 26.027 511 280 12 64 541 36 481 1.16e-41 159
MS.gene027737.t1 MTR_4g074190 26.282 468 267 12 27 470 4 417 1.91e-35 139
MS.gene027737.t1 MTR_1g105870 32.161 199 119 4 33 230 38 221 5.94e-21 93.6
MS.gene027737.t1 MTR_2g010890 25.060 419 246 10 94 505 1 358 6.28e-21 96.7
MS.gene027737.t1 MTR_4g080720 64.912 57 20 0 1 57 1 57 1.43e-19 83.6
MS.gene027737.t1 MTR_2g048375 36.190 105 50 4 485 586 1 91 8.15e-12 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene027737.t1 AT1G67900 72.868 645 151 11 1 636 1 630 0.0 864
MS.gene027737.t1 AT1G67900 72.868 645 151 11 1 636 1 630 0.0 864
MS.gene027737.t1 AT1G67900 72.868 645 151 11 1 636 1 630 0.0 864
MS.gene027737.t1 AT1G67900 72.868 645 151 11 1 636 1 630 0.0 864
MS.gene027737.t1 AT1G67900 72.868 645 151 11 1 636 1 630 0.0 864
MS.gene027737.t1 AT1G67900 72.868 645 151 11 1 636 1 630 0.0 864
MS.gene027737.t1 AT3G26490 49.085 656 244 15 1 635 1 587 0.0 560
MS.gene027737.t1 AT3G26490 49.085 656 244 15 1 635 1 587 0.0 560
MS.gene027737.t1 AT3G26490 49.085 656 244 15 1 635 1 587 0.0 560
MS.gene027737.t1 AT3G26490 53.061 490 176 13 1 469 1 457 8.86e-161 471
MS.gene027737.t1 AT2G14820 45.149 536 247 8 1 528 1 497 5.20e-152 453
MS.gene027737.t1 AT2G14820 45.149 536 247 8 1 528 1 497 5.20e-152 453
MS.gene027737.t1 AT5G47800 43.152 533 257 7 1 530 1 490 4.24e-147 438
MS.gene027737.t1 AT5G47800 43.152 533 257 7 1 530 1 490 4.24e-147 438
MS.gene027737.t1 AT5G47800 43.152 533 257 7 1 530 1 490 4.24e-147 438
MS.gene027737.t1 AT5G47800 43.152 533 257 7 1 530 114 603 2.50e-145 437
MS.gene027737.t1 AT5G67440 43.619 525 247 8 1 522 1 479 5.08e-143 428
MS.gene027737.t1 AT5G67440 43.619 525 247 8 1 522 1 479 5.08e-143 428
MS.gene027737.t1 AT4G37590 40.336 595 263 12 1 582 1 516 4.14e-141 423
MS.gene027737.t1 AT4G31820 42.533 529 241 10 1 523 1 472 1.28e-132 401
MS.gene027737.t1 AT5G67440 43.595 484 225 7 41 522 5 442 3.44e-128 389
MS.gene027737.t1 AT2G23050 41.588 529 228 11 1 523 1 454 2.14e-127 384
MS.gene027737.t1 AT5G64330 35.224 670 348 12 7 599 33 693 3.07e-123 382
MS.gene027737.t1 AT5G64330 36.661 611 313 9 7 549 33 637 1.11e-122 379
MS.gene027737.t1 AT5G64330 37.306 579 289 9 39 549 6 578 3.25e-117 362
MS.gene027737.t1 AT4G31820 41.807 476 215 9 53 523 1 419 4.79e-116 357
MS.gene027737.t1 AT1G30440 39.065 535 291 9 1 523 1 512 7.84e-115 358
MS.gene027737.t1 AT5G03250 37.594 532 297 6 1 523 1 506 2.23e-113 352
MS.gene027737.t1 AT5G13600 40.377 530 276 10 1 523 1 497 1.64e-111 347
MS.gene027737.t1 AT5G67385 38.976 508 258 8 15 519 16 474 6.66e-106 333
MS.gene027737.t1 AT5G48800 39.053 507 249 9 22 523 37 488 9.43e-99 315
MS.gene027737.t1 AT5G67385 38.025 476 245 7 47 519 1 429 9.71e-97 308
MS.gene027737.t1 AT5G67385 38.025 476 245 7 47 519 1 429 9.71e-97 308
MS.gene027737.t1 AT5G67385 38.025 476 245 7 47 519 1 429 9.71e-97 308
MS.gene027737.t1 AT5G67385 38.025 476 245 7 47 519 1 429 9.71e-97 308
MS.gene027737.t1 AT5G10250 34.549 521 258 8 22 519 46 506 3.72e-93 300
MS.gene027737.t1 AT5G66560 36.213 544 257 10 28 517 21 528 4.30e-93 301
MS.gene027737.t1 AT3G44820 33.956 589 329 15 26 591 23 574 2.53e-92 299
MS.gene027737.t1 AT3G08570 37.255 510 276 11 13 519 22 490 4.00e-92 297
MS.gene027737.t1 AT5G66560 36.210 533 250 10 39 517 3 499 2.63e-91 296
MS.gene027737.t1 AT5G66560 38.913 460 233 9 70 517 66 489 4.98e-91 295
MS.gene027737.t1 AT1G03010 37.972 503 272 12 24 519 7 476 1.45e-90 293
MS.gene027737.t1 AT1G03010 37.597 516 280 13 11 519 22 502 3.58e-90 293
MS.gene027737.t1 AT1G03010 37.972 503 272 12 24 519 34 503 4.82e-90 292
MS.gene027737.t1 AT5G10250 34.638 511 256 9 22 519 46 491 6.01e-90 291
MS.gene027737.t1 AT2G23050 40.436 413 169 8 113 523 1 338 4.45e-87 276
MS.gene027737.t1 AT3G49970 36.344 465 216 11 39 502 7 392 1.63e-86 280
MS.gene027737.t1 AT3G49970 36.344 465 216 11 39 502 7 392 1.63e-86 280
MS.gene027737.t1 AT3G08660 35.000 520 256 12 8 519 21 466 3.90e-84 275
MS.gene027737.t1 AT2G47860 35.341 498 282 10 24 519 35 494 4.75e-81 269
MS.gene027737.t1 AT2G30520 32.604 503 285 9 21 523 25 473 3.75e-79 263
MS.gene027737.t1 AT3G50840 34.921 504 276 8 28 529 18 471 2.93e-78 259
MS.gene027737.t1 AT5G03250 36.190 420 233 6 113 523 1 394 2.53e-76 252
MS.gene027737.t1 AT2G47860 33.460 526 277 11 24 519 35 517 6.78e-76 255
MS.gene027737.t1 AT3G08660 35.076 459 224 10 69 519 14 406 3.20e-74 247
MS.gene027737.t1 AT2G30520 33.260 454 251 8 70 523 24 425 7.70e-73 244
MS.gene027737.t1 AT2G30520 33.260 454 251 8 70 523 24 425 7.70e-73 244
MS.gene027737.t1 AT3G50840 34.227 485 267 8 47 529 1 435 7.50e-72 241
MS.gene027737.t1 AT3G50840 34.227 485 267 8 47 529 1 435 7.50e-72 241
MS.gene027737.t1 AT5G66560 32.866 499 239 10 28 469 21 480 1.77e-66 226
MS.gene027737.t1 AT1G52770 39.677 310 152 4 211 519 28 303 5.34e-64 218
MS.gene027737.t1 AT3G08660 31.770 469 242 12 8 470 21 417 1.56e-61 211
MS.gene027737.t1 AT3G15570 37.705 305 151 4 220 519 38 308 6.50e-59 204
MS.gene027737.t1 AT2G47860 32.662 447 261 10 24 468 35 443 1.56e-57 201
MS.gene027737.t1 AT2G47860 33.092 414 232 9 113 519 1 376 1.74e-54 194
MS.gene027737.t1 AT2G47860 33.092 414 232 9 113 519 1 376 1.74e-54 194
MS.gene027737.t1 AT2G47860 30.998 471 262 11 24 469 35 467 1.12e-52 188
MS.gene027737.t1 AT3G19850 26.105 475 263 8 69 517 51 463 8.76e-49 179
MS.gene027737.t1 AT5G48130 25.828 453 270 9 70 517 76 467 9.96e-42 160
MS.gene027737.t1 AT3G03510 30.000 450 207 12 82 519 8 361 1.18e-41 157
MS.gene027737.t1 AT1G50280 24.353 464 277 8 70 513 18 427 1.39e-39 152
MS.gene027737.t1 AT1G50280 24.353 464 277 8 70 513 51 460 1.62e-39 152
MS.gene027737.t1 AT5G17580 27.567 526 311 11 8 526 2 464 1.71e-38 150
MS.gene027737.t1 AT5G17580 27.734 512 300 11 22 526 1 449 4.38e-38 149
MS.gene027737.t1 AT3G22104 25.773 485 278 13 28 502 6 418 9.65e-32 129
MS.gene027737.t1 AT3G22104 25.182 413 230 10 98 502 2 343 1.37e-22 101
MS.gene027737.t1 AT3G22104 25.182 413 230 10 98 502 2 343 1.37e-22 101
MS.gene027737.t1 AT3G22104 25.182 413 230 10 98 502 2 343 1.37e-22 101

Find 113 sgRNAs with CRISPR-Local

Find 193 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACTTAGCATAGACCTTTAT+TGG 0.142674 5.2:+22778559 MS.gene027737:CDS
GAGGATAATTGGAGTGTTTC+TGG 0.144528 5.2:+22780649 MS.gene027737:CDS
CTTCTTTCTATTTCCGTTCT+TGG 0.164934 5.2:-22778679 None:intergenic
CTTTCACTCTTGTTGAGTTC+TGG 0.171118 5.2:-22780376 None:intergenic
TTTCCGTTCTTGGAAATATT+TGG 0.180699 5.2:-22778669 None:intergenic
AATCAGGAAGTTGGACTATT+TGG 0.186197 5.2:-22778110 None:intergenic
CATGATGAATTTGATGAAAA+TGG 0.208213 5.2:+22780727 MS.gene027737:CDS
ACTTGAACAACAACTCTTAA+AGG 0.232478 5.2:-22780478 None:intergenic
GCATTCAAGGTGAGGATAAT+TGG 0.282822 5.2:+22780638 MS.gene027737:CDS
TCCAAGTTTCATAAACTTCA+TGG 0.285144 5.2:-22777495 None:intergenic
AGAGTTGAAGACTTTGATTT+TGG 0.291628 5.2:-22780682 None:intergenic
TTTCAACTTCTTCAGTCATT+TGG 0.299710 5.2:-22778242 None:intergenic
TGATCATATCTTGCAAATTC+TGG 0.304119 5.2:-22779254 None:intergenic
AGCTTGCTTCCAACCGAAAA+AGG 0.306769 5.2:+22778789 MS.gene027737:CDS
TGAAGAAGCATTGAGAATAT+TGG 0.313880 5.2:-22778853 None:intergenic
TCAAAAGTCTTCAACGATCT+TGG 0.319797 5.2:-22779050 None:intergenic
TTCAAAGCATAGGCTTCTAT+TGG 0.325252 5.2:+22778755 MS.gene027737:CDS
ATGACTGAAGAAGTTGAAAA+AGG 0.332313 5.2:+22778246 MS.gene027737:CDS
TCTACACTTCAGAAGCTATA+AGG 0.334248 5.2:+22777534 MS.gene027737:CDS
CTATCATTGTTCTCCAATAA+AGG 0.335482 5.2:-22778572 None:intergenic
TGAAAATTTATGCATCTAGA+TGG 0.337434 5.2:+22778643 MS.gene027737:CDS
TCTGAAGTGTAGAAAGTATC+TGG 0.344925 5.2:-22777524 None:intergenic
GCCATGAAGTTTATGAAACT+TGG 0.345527 5.2:+22777494 None:intergenic
CTACAAAAGCGTTGCATTTG+TGG 0.358213 5.2:+22778349 MS.gene027737:CDS
TGTAGTCTGAAGACTTACTA+TGG 0.359741 5.2:-22778331 None:intergenic
TCCACTCCACCCGGAAAATC+AGG 0.369267 5.2:-22778126 None:intergenic
TTGGTAAATAATGATGGAAT+TGG 0.369972 5.2:+22780751 MS.gene027737:CDS
TGAAAGAAGGCGGTCGCGTT+CGG 0.375582 5.2:+22779070 MS.gene027737:CDS
TGTGACAAACTCACCTTTGT+AGG 0.375606 5.2:-22778435 None:intergenic
TCTTTGCAGGCATAAGCATT+TGG 0.386345 5.2:-22778052 None:intergenic
ATTCTGAGCATAATCTTTGC+AGG 0.392741 5.2:-22778065 None:intergenic
TGAGTTGGTGATGACTATAT+TGG 0.420160 5.2:+22778986 MS.gene027737:CDS
GTGATCTCATAATTCAAGTA+AGG 0.425248 5.2:+22777683 MS.gene027737:CDS
TGCTTCTTCATCTTCAAAGA+TGG 0.426814 5.2:+22778866 MS.gene027737:CDS
GCATTTGTGGTCCGAAGACT+TGG 0.427763 5.2:+22778362 MS.gene027737:CDS
CTTGAACAACAACTCTTAAA+GGG 0.429190 5.2:-22780477 None:intergenic
AGCTCTACTACAAGCATTCA+AGG 0.437283 5.2:+22780625 MS.gene027737:CDS
AAGAAGAGTAGGACTTCAAT+TGG 0.440636 5.2:+22778896 MS.gene027737:CDS
TGTCACATAGTCATTCTAGA+AGG 0.441367 5.2:+22778451 MS.gene027737:CDS
GCATCACCAATGAGATTTGA+AGG 0.447840 5.2:-22778618 None:intergenic
GGCATAAGCATTTGGACAGC+AGG 0.452818 5.2:-22778044 None:intergenic
TTGTGTAGGAAGAGCACAAA+TGG 0.459576 5.2:-22780794 None:intergenic
TGAATTTGATGAAAATGGTT+TGG 0.459746 5.2:+22780732 MS.gene027737:CDS
ACTTGTGGATAGGTACCTTC+AGG 0.460283 5.2:+22779172 MS.gene027737:CDS
CAAGCTAGAGCAGCATCATC+TGG 0.460416 5.2:+22780514 MS.gene027737:CDS
ATTTGGTGATGAGATGAATC+AGG 0.463874 5.2:-22778093 None:intergenic
TACCTTGAGATAAATGTCAA+TGG 0.464242 5.2:-22779294 None:intergenic
ACAATGATAGCAATCAAATC+TGG 0.464906 5.2:+22778585 MS.gene027737:CDS
AAGGTTCCTTCAAATCTCAT+TGG 0.467784 5.2:+22778612 MS.gene027737:CDS
TTCAATTCTTGCATCCTCCA+TGG 0.467888 5.2:+22778297 MS.gene027737:CDS
ATGCTTTGAAGTTATTTCGC+TGG 0.473982 5.2:-22778742 None:intergenic
AACGATCTTGGAGGGCTAGT+TGG 0.475483 5.2:-22779038 None:intergenic
GAAAGTTTGAGAATCAAATC+AGG 0.487700 5.2:+22778504 MS.gene027737:CDS
GCTGGTAATTCCCAAGTCTT+CGG 0.495511 5.2:-22778373 None:intergenic
TTGGAACATTTCATGTTACA+AGG 0.498296 5.2:+22779005 MS.gene027737:CDS
AAATGTCAATGGCTCTATAG+AGG 0.499230 5.2:-22779283 None:intergenic
AGAATCAAATCAGGAAGTAA+AGG 0.499839 5.2:+22778513 MS.gene027737:CDS
CAAAAGAAAGCTACAAGAAA+CGG 0.503747 5.2:-22778814 None:intergenic
AAAGTCTTCAACGATCTTGG+AGG 0.513419 5.2:-22779047 None:intergenic
AATGGTTTGGTAAATAATGA+TGG 0.516085 5.2:+22780745 MS.gene027737:CDS
ATTCTTGCATCCTCCATGGT+TGG 0.519380 5.2:+22778301 MS.gene027737:CDS
CACCCGGAAAATCAGGAAGT+TGG 0.520809 5.2:-22778119 None:intergenic
ATTGAAAGTGGCAAAACTTG+TGG 0.529222 5.2:+22779157 MS.gene027737:CDS
GCGAAATAACTTCAAAGCAT+AGG 0.531559 5.2:+22778745 MS.gene027737:CDS
ATGGAATTAGCAAGAAGAGT+AGG 0.536555 5.2:+22778885 MS.gene027737:CDS
TTGCATCCTCCATGGTTGGA+AGG 0.536990 5.2:+22778305 MS.gene027737:CDS
CTTGCAGCTACAATGTTGTA+AGG 0.538418 5.2:-22778201 None:intergenic
AATAGTTTCAATGCATCTGC+TGG 0.540399 5.2:-22778391 None:intergenic
ACATAGTTCAAAATTGAAAG+TGG 0.541920 5.2:+22779145 MS.gene027737:CDS
TTGAATTACAAGAGAGTAGG+AGG 0.548073 5.2:+22779108 MS.gene027737:CDS
ACTTTGAATTACAAGAGAGT+AGG 0.548128 5.2:+22779105 MS.gene027737:CDS
GATCTTGGAGGGCTAGTTGG+TGG 0.550378 5.2:-22779035 None:intergenic
AGCCATTGACATTTATCTCA+AGG 0.550508 5.2:+22779292 MS.gene027737:CDS
TGATCTCATAATTCAAGTAA+GGG 0.550839 5.2:+22777684 MS.gene027737:CDS
AATGGAAGCGTGCATGCATG+CGG 0.551671 5.2:+22780438 MS.gene027737:CDS
GTGACAAACTCACCTTTGTA+GGG 0.551753 5.2:-22778434 None:intergenic
TTGTGTGCTAAGTTCTGCTA+TGG 0.551870 5.2:+22778159 MS.gene027737:CDS
AAACTTGATTCACAAGCTTG+AGG 0.555629 5.2:+22778269 MS.gene027737:CDS
TTACCAAATATTTCCAAGAA+CGG 0.560310 5.2:+22778666 MS.gene027737:CDS
CAGAACTCAACAAGAGTGAA+AGG 0.560948 5.2:+22780377 MS.gene027737:CDS
TGAACTATGTGATGCTGAAG+AGG 0.563744 5.2:-22779132 None:intergenic
GCATAAGCATTTGGACAGCA+GGG 0.571856 5.2:-22778043 None:intergenic
TCAAATGCTTCCACTCCACC+CGG 0.574131 5.2:-22778135 None:intergenic
AAGTGGCAAAACTTGTGGAT+AGG 0.577058 5.2:+22779162 MS.gene027737:CDS
ATAGTCATTCTAGAAGGGTG+AGG 0.577522 5.2:+22778457 MS.gene027737:CDS
CATTTGTGGTCCGAAGACTT+GGG 0.581251 5.2:+22778363 MS.gene027737:CDS
AAGAGTAGGACTTCAATTGG+AGG 0.582077 5.2:+22778899 MS.gene027737:CDS
ATCAGGAAGTAAAGGATGGT+GGG 0.583008 5.2:+22778521 MS.gene027737:CDS
AGCAATCAAATCTGGTGGCA+AGG 0.587341 5.2:+22778593 MS.gene027737:CDS
CTATGGTGTCCTTCCAACCA+TGG 0.587903 5.2:-22778314 None:intergenic
GTCACATAGTCATTCTAGAA+GGG 0.589043 5.2:+22778452 MS.gene027737:CDS
TACTACAAGCATTCAAGGTG+AGG 0.589288 5.2:+22780630 MS.gene027737:CDS
CATCTCTTGCAACTTCCTGA+AGG 0.592567 5.2:-22779187 None:intergenic
GTCTGAGTCTAACTCAGACT+CGG 0.598165 5.2:+22778719 MS.gene027737:CDS
TAGTCATTCTAGAAGGGTGA+GGG 0.603321 5.2:+22778458 MS.gene027737:CDS
AATCAGGAAGTAAAGGATGG+TGG 0.603373 5.2:+22778520 MS.gene027737:CDS
AGTCTTGAGCCACCCTACAA+AGG 0.609787 5.2:+22778422 MS.gene027737:CDS
TCAAATCAGGAAGTAAAGGA+TGG 0.616909 5.2:+22778517 MS.gene027737:CDS
AAGTCTTCAACGATCTTGGA+GGG 0.617841 5.2:-22779046 None:intergenic
TACAGTCTATGATGTTGAGT+TGG 0.618402 5.2:+22778971 MS.gene027737:CDS
ATGATAGCAATCAAATCTGG+TGG 0.634663 5.2:+22778588 MS.gene027737:CDS
GCTAGAGCAGCATCATCTGG+TGG 0.637791 5.2:+22780517 MS.gene027737:CDS
GATCTCATAATTCAAGTAAG+GGG 0.642036 5.2:+22777685 MS.gene027737:CDS
GTCTGAGTTAGACTCAGACT+CGG 0.643618 5.2:-22778715 None:intergenic
AAAGCATTGCTTACAACACA+TGG 0.648620 5.2:+22780568 MS.gene027737:CDS
TCTAAGAATGAAGCTAGCTG+AGG 0.667457 5.2:+22780702 MS.gene027737:CDS
CATAAGCATTTGGACAGCAG+GGG 0.670987 5.2:-22778042 None:intergenic
ACAAACTCACCTTTGTAGGG+TGG 0.674969 5.2:-22778431 None:intergenic
TCATTTGTATAAGAAAGTGA+AGG 0.677583 5.2:-22778948 None:intergenic
AAGTAGATATCTTCTTCACA+AGG 0.678976 5.2:+22777708 MS.gene027737:CDS
TGGTGTCCTTCCAACCATGG+AGG 0.717381 5.2:-22778311 None:intergenic
TGGTGATGAGATGAATCAGG+AGG 0.725233 5.2:-22778090 None:intergenic
TATTAACAGATCATTCACTG+TGG 0.785745 5.2:-22778925 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATACAATAAACATTTTAT+AGG - chr5.2:22779329-22779348 None:intergenic 10.0%
!!! AAGTATATAATTTTTTACTT+TGG + chr5.2:22777982-22778001 MS.gene027737:intron 10.0%
!!! TTAATTTAAGTTTTTTTTCA+AGG + chr5.2:22779995-22780014 MS.gene027737:intron 10.0%
!! AAAAACATGTAAAAAAAAAG+TGG + chr5.2:22779537-22779556 MS.gene027737:intron 15.0%
!!! AATTTACTAAGCATTTTTTT+AGG - chr5.2:22780820-22780839 None:intergenic 15.0%
!!! ATTTTTACCAAGTTATAAAA+TGG - chr5.2:22779364-22779383 None:intergenic 15.0%
!!! TTATGTGTGTATTTTTTTAT+AGG - chr5.2:22779518-22779537 None:intergenic 15.0%
!!! TTGAATAAGACAATATTTTA+AGG - chr5.2:22779636-22779655 None:intergenic 15.0%
!! AAAAAATGCTTAGTAAATTG+TGG + chr5.2:22780821-22780840 MS.gene027737:CDS 20.0%
!! AAATAGAGTGAATTCATAAA+TGG - chr5.2:22779894-22779913 None:intergenic 20.0%
!! AGAAAATTTCATAACTCATT+TGG + chr5.2:22779748-22779767 MS.gene027737:intron 20.0%
!! CAAAATTAAACAATCCAAAA+AGG - chr5.2:22777610-22777629 None:intergenic 20.0%
!!! ATACTTCACTTTCAAATTTT+AGG + chr5.2:22779573-22779592 MS.gene027737:intron 20.0%
!!! TTGTCTTATTCAACATTTAT+TGG + chr5.2:22779643-22779662 MS.gene027737:intron 20.0%
! AAAATAACGAGCTTGAAATA+TGG - chr5.2:22780221-22780240 None:intergenic 25.0%
! ACATAGTTCAAAATTGAAAG+TGG + chr5.2:22779145-22779164 MS.gene027737:CDS 25.0%
! ACTAATATGACACATTGATA+CGG - chr5.2:22777907-22777926 None:intergenic 25.0%
! ATAGCCTAATTAATTGAACA+TGG - chr5.2:22780082-22780101 None:intergenic 25.0%
! ATGTTCAATTAATTAGGCTA+TGG + chr5.2:22780081-22780100 MS.gene027737:intron 25.0%
! CATGATGAATTTGATGAAAA+TGG + chr5.2:22780727-22780746 MS.gene027737:CDS 25.0%
! GAAGAAAAATTTATAGCTTG+AGG + chr5.2:22780103-22780122 MS.gene027737:intron 25.0%
! TCATTTGTATAAGAAAGTGA+AGG - chr5.2:22778951-22778970 None:intergenic 25.0%
! TGAAAATTTATGCATCTAGA+TGG + chr5.2:22778643-22778662 MS.gene027737:CDS 25.0%
! TGAATTTGATGAAAATGGTT+TGG + chr5.2:22780732-22780751 MS.gene027737:CDS 25.0%
! TGATCTCATAATTCAAGTAA+GGG + chr5.2:22777684-22777703 MS.gene027737:CDS 25.0%
! TGGTAAAAATATATCTCAGA+TGG + chr5.2:22779374-22779393 MS.gene027737:intron 25.0%
! TTACCAAATATTTCCAAGAA+CGG + chr5.2:22778666-22778685 MS.gene027737:CDS 25.0%
! TTCAACATTTATTGGAAGTT+AGG + chr5.2:22779651-22779670 MS.gene027737:intron 25.0%
!! AAATTTTCTGAACATGTTAC+TGG - chr5.2:22779737-22779756 None:intergenic 25.0%
!! TTGGTAAATAATGATGGAAT+TGG + chr5.2:22780751-22780770 MS.gene027737:CDS 25.0%
!! TTTCTTAATTTGAAACATGC+AGG + chr5.2:22780348-22780367 MS.gene027737:intron 25.0%
!!! AATGGTTTGGTAAATAATGA+TGG + chr5.2:22780745-22780764 MS.gene027737:CDS 25.0%
AACTTAGCATAGACCTTTAT+TGG + chr5.2:22778559-22778578 MS.gene027737:CDS 30.0%
AAGTAGATATCTTCTTCACA+AGG + chr5.2:22777708-22777727 MS.gene027737:CDS 30.0%
ACAATGATAGCAATCAAATC+TGG + chr5.2:22778585-22778604 MS.gene027737:CDS 30.0%
ACTTGAACAACAACTCTTAA+AGG - chr5.2:22780481-22780500 None:intergenic 30.0%
ACTTTCTTAATACTCGTTGA+GGG - chr5.2:22779425-22779444 None:intergenic 30.0%
ACTTTGAATTACAAGAGAGT+AGG + chr5.2:22779105-22779124 MS.gene027737:CDS 30.0%
AGAATCAAATCAGGAAGTAA+AGG + chr5.2:22778513-22778532 MS.gene027737:CDS 30.0%
AGGATATGAAACAGAATTAG+TGG + chr5.2:22779593-22779612 MS.gene027737:intron 30.0%
ATGACTGAAGAAGTTGAAAA+AGG + chr5.2:22778246-22778265 MS.gene027737:CDS 30.0%
ATTAATTGAACATGGTAGCT+CGG - chr5.2:22780074-22780093 None:intergenic 30.0%
CAAAAGAAAGCTACAAGAAA+CGG - chr5.2:22778817-22778836 None:intergenic 30.0%
CATCATTATAGAGTTCATTG+AGG - chr5.2:22779929-22779948 None:intergenic 30.0%
CTATCATTGTTCTCCAATAA+AGG - chr5.2:22778575-22778594 None:intergenic 30.0%
CTTGAACAACAACTCTTAAA+GGG - chr5.2:22780480-22780499 None:intergenic 30.0%
GAAAGTTTGAGAATCAAATC+AGG + chr5.2:22778504-22778523 MS.gene027737:CDS 30.0%
GAACTATATCAGTCAAGATA+AGG - chr5.2:22779715-22779734 None:intergenic 30.0%
GATCTCATAATTCAAGTAAG+GGG + chr5.2:22777685-22777704 MS.gene027737:CDS 30.0%
GCTACCATGTTCAATTAATT+AGG + chr5.2:22780075-22780094 MS.gene027737:intron 30.0%
GTGATCTCATAATTCAAGTA+AGG + chr5.2:22777683-22777702 MS.gene027737:CDS 30.0%
TACCTTGAGATAAATGTCAA+TGG - chr5.2:22779297-22779316 None:intergenic 30.0%
TATTAACAGATCATTCACTG+TGG - chr5.2:22778928-22778947 None:intergenic 30.0%
TGATCATATCTTGCAAATTC+TGG - chr5.2:22779257-22779276 None:intergenic 30.0%
TTGGAACATTTCATGTTACA+AGG + chr5.2:22779005-22779024 MS.gene027737:CDS 30.0%
TTTCAACTTCTTCAGTCATT+TGG - chr5.2:22778245-22778264 None:intergenic 30.0%
TTTCCGTTCTTGGAAATATT+TGG - chr5.2:22778672-22778691 None:intergenic 30.0%
! AGTCACTCCATTTTATAACT+TGG + chr5.2:22779354-22779373 MS.gene027737:intron 30.0%
! GTTCTTTTCTTTGTATATGC+AGG + chr5.2:22777638-22777657 MS.gene027737:intron 30.0%
!! AGAGTTGAAGACTTTGATTT+TGG - chr5.2:22780685-22780704 None:intergenic 30.0%
!! TGAAGAAGCATTGAGAATAT+TGG - chr5.2:22778856-22778875 None:intergenic 30.0%
!! TGTGTTGTTGTATGTTTTGA+TGG - chr5.2:22780601-22780620 None:intergenic 30.0%
!!! AGCATTTTTTTAGGTTGTGT+AGG - chr5.2:22780811-22780830 None:intergenic 30.0%
!!! TATTTTAAGGTCAAGAGAGT+TGG - chr5.2:22779623-22779642 None:intergenic 30.0%
AAACTTGATTCACAAGCTTG+AGG + chr5.2:22778269-22778288 MS.gene027737:CDS 35.0%
AAGAAGAGTAGGACTTCAAT+TGG + chr5.2:22778896-22778915 MS.gene027737:CDS 35.0%
AAGGTTCCTTCAAATCTCAT+TGG + chr5.2:22778612-22778631 MS.gene027737:CDS 35.0%
AATAGTTTCAATGCATCTGC+TGG - chr5.2:22778394-22778413 None:intergenic 35.0%
AATCAGGAAGTTGGACTATT+TGG - chr5.2:22778113-22778132 None:intergenic 35.0%
ACGTATTGTTACATTTCGGA+AGG + chr5.2:22777857-22777876 MS.gene027737:intron 35.0%
AGCCATTGACATTTATCTCA+AGG + chr5.2:22779292-22779311 MS.gene027737:CDS 35.0%
ATAGGTGAATATGTGACTAC+TGG + chr5.2:22780282-22780301 MS.gene027737:intron 35.0%
ATGATAGCAATCAAATCTGG+TGG + chr5.2:22778588-22778607 MS.gene027737:CDS 35.0%
ATGGAATTAGCAAGAAGAGT+AGG + chr5.2:22778885-22778904 MS.gene027737:CDS 35.0%
ATTAGTAGTTCGTTACGAGA+AGG - chr5.2:22779483-22779502 None:intergenic 35.0%
ATTCTGAGCATAATCTTTGC+AGG - chr5.2:22778068-22778087 None:intergenic 35.0%
ATTGAAAGTGGCAAAACTTG+TGG + chr5.2:22779157-22779176 MS.gene027737:CDS 35.0%
CACACTATCAATTCATGAGA+AGG - chr5.2:22779842-22779861 None:intergenic 35.0%
CACTTTCTTAATACTCGTTG+AGG - chr5.2:22779426-22779445 None:intergenic 35.0%
CATTATAGAGTTCATTGAGG+TGG - chr5.2:22779926-22779945 None:intergenic 35.0%
CATTTATTGGAAGTTAGGTG+TGG + chr5.2:22779656-22779675 MS.gene027737:intron 35.0%
CTCAACGAGTATTAAGAAAG+TGG + chr5.2:22779424-22779443 MS.gene027737:intron 35.0%
CTTCTTTCTATTTCCGTTCT+TGG - chr5.2:22778682-22778701 None:intergenic 35.0%
GCGAAATAACTTCAAAGCAT+AGG + chr5.2:22778745-22778764 MS.gene027737:CDS 35.0%
GCTGTTAGATCAAAATTCGA+CGG + chr5.2:22780194-22780213 MS.gene027737:intron 35.0%
TACAGTCTATGATGTTGAGT+TGG + chr5.2:22778971-22778990 MS.gene027737:CDS 35.0%
TCAAAAGTCTTCAACGATCT+TGG - chr5.2:22779053-22779072 None:intergenic 35.0%
TCAAATCAGGAAGTAAAGGA+TGG + chr5.2:22778517-22778536 MS.gene027737:CDS 35.0%
TCTACACTTCAGAAGCTATA+AGG + chr5.2:22777534-22777553 MS.gene027737:CDS 35.0%
TGCTTCTTCATCTTCAAAGA+TGG + chr5.2:22778866-22778885 MS.gene027737:CDS 35.0%
TGTAGTCTGAAGACTTACTA+TGG - chr5.2:22778334-22778353 None:intergenic 35.0%
TGTCACATAGTCATTCTAGA+AGG + chr5.2:22778451-22778470 MS.gene027737:CDS 35.0%
TGTCATATTAGTGTTGTCTC+TGG + chr5.2:22777915-22777934 MS.gene027737:intron 35.0%
TTAGTAGTTCGTTACGAGAA+GGG - chr5.2:22779482-22779501 None:intergenic 35.0%
TTCAAAGCATAGGCTTCTAT+TGG + chr5.2:22778755-22778774 MS.gene027737:CDS 35.0%
TTGAATTACAAGAGAGTAGG+AGG + chr5.2:22779108-22779127 MS.gene027737:CDS 35.0%
TTGATAGAAGGGATTAAGCA+CGG - chr5.2:22779452-22779471 None:intergenic 35.0%
! AAATGTCAATGGCTCTATAG+AGG - chr5.2:22779286-22779305 None:intergenic 35.0%
! AAGGGGAAATTGTTGATAGA+AGG - chr5.2:22779464-22779483 None:intergenic 35.0%
! AGGGGAAATTGTTGATAGAA+GGG - chr5.2:22779463-22779482 None:intergenic 35.0%
! GTCACATAGTCATTCTAGAA+GGG + chr5.2:22778452-22778471 MS.gene027737:CDS 35.0%
! TGAGGCGTTATTGTAATGAT+TGG + chr5.2:22780121-22780140 MS.gene027737:intron 35.0%
!! AAAGCATTGCTTACAACACA+TGG + chr5.2:22780568-22780587 MS.gene027737:CDS 35.0%
!! ATGCTTTGAAGTTATTTCGC+TGG - chr5.2:22778745-22778764 None:intergenic 35.0%
!! ATTTGGTGATGAGATGAATC+AGG - chr5.2:22778096-22778115 None:intergenic 35.0%
!! CGTTGAAGACTTTTGAAAGA+AGG + chr5.2:22779057-22779076 MS.gene027737:CDS 35.0%
!! TCTGAAGTGTAGAAAGTATC+TGG - chr5.2:22777527-22777546 None:intergenic 35.0%
!! TGAAGACTTTTGAAAGAAGG+CGG + chr5.2:22779060-22779079 MS.gene027737:CDS 35.0%
!! TGAGTTGGTGATGACTATAT+TGG + chr5.2:22778986-22779005 MS.gene027737:CDS 35.0%
!!! TTGATGATGCATCTCCTTTT+TGG + chr5.2:22777593-22777612 MS.gene027737:intron 35.0%
AAAGTCTTCAACGATCTTGG+AGG - chr5.2:22779050-22779069 None:intergenic 40.0%
AAGAGTAGGACTTCAATTGG+AGG + chr5.2:22778899-22778918 MS.gene027737:CDS 40.0%
AAGTCTTCAACGATCTTGGA+GGG - chr5.2:22779049-22779068 None:intergenic 40.0%
AAGTGGCAAAACTTGTGGAT+AGG + chr5.2:22779162-22779181 MS.gene027737:CDS 40.0%
AATCAGGAAGTAAAGGATGG+TGG + chr5.2:22778520-22778539 MS.gene027737:CDS 40.0%
AGCTCTACTACAAGCATTCA+AGG + chr5.2:22780625-22780644 MS.gene027737:CDS 40.0%
ATCAGGAAGTAAAGGATGGT+GGG + chr5.2:22778521-22778540 MS.gene027737:CDS 40.0%
CAGAACTCAACAAGAGTGAA+AGG + chr5.2:22780377-22780396 MS.gene027737:CDS 40.0%
CGAAATGTAACAATACGTGC+CGG - chr5.2:22777855-22777874 None:intergenic 40.0%
CGGCACGTATTGTTACATTT+CGG + chr5.2:22777853-22777872 MS.gene027737:intron 40.0%
CTTGCAGCTACAATGTTGTA+AGG - chr5.2:22778204-22778223 None:intergenic 40.0%
CTTTCACTCTTGTTGAGTTC+TGG - chr5.2:22780379-22780398 None:intergenic 40.0%
GAGTTCATTGAGGTGGAAAT+CGG - chr5.2:22779919-22779938 None:intergenic 40.0%
GCACAGACACTTCAAATTGA+AGG + chr5.2:22777803-22777822 MS.gene027737:intron 40.0%
GCATCACCAATGAGATTTGA+AGG - chr5.2:22778621-22778640 None:intergenic 40.0%
GCATTCAAGGTGAGGATAAT+TGG + chr5.2:22780638-22780657 MS.gene027737:CDS 40.0%
GTCAGTCAGGCTAAAATTTC+TGG - chr5.2:22779691-22779710 None:intergenic 40.0%
GTGACAAACTCACCTTTGTA+GGG - chr5.2:22778437-22778456 None:intergenic 40.0%
TACTACAAGCATTCAAGGTG+AGG + chr5.2:22780630-22780649 MS.gene027737:CDS 40.0%
TAGTAGTTCGTTACGAGAAG+GGG - chr5.2:22779481-22779500 None:intergenic 40.0%
TCATGAGAAGGACATAGTAC+TGG - chr5.2:22779830-22779849 None:intergenic 40.0%
TCTAAGAATGAAGCTAGCTG+AGG + chr5.2:22780702-22780721 MS.gene027737:CDS 40.0%
TCTTTGCAGGCATAAGCATT+TGG - chr5.2:22778055-22778074 None:intergenic 40.0%
TGAACTATGTGATGCTGAAG+AGG - chr5.2:22779135-22779154 None:intergenic 40.0%
TGACTGCAAAAAGCTCTCAA+TGG + chr5.2:22780420-22780439 MS.gene027737:CDS 40.0%
TGTGACAAACTCACCTTTGT+AGG - chr5.2:22778438-22778457 None:intergenic 40.0%
TTCAATTCTTGCATCCTCCA+TGG + chr5.2:22778297-22778316 MS.gene027737:CDS 40.0%
TTGTGTGCTAAGTTCTGCTA+TGG + chr5.2:22778159-22778178 MS.gene027737:CDS 40.0%
! ACATAGTACTGGAACTGTGA+TGG - chr5.2:22779819-22779838 None:intergenic 40.0%
! ACTGAATGAGTTCTAACAGG+AGG - chr5.2:22780034-22780053 None:intergenic 40.0%
! AGTCAGGCTAAAATTTCTGG+TGG - chr5.2:22779688-22779707 None:intergenic 40.0%
! AGTCCAACTTCCTGATTTTC+CGG + chr5.2:22778116-22778135 MS.gene027737:CDS 40.0%
! ATAGTCATTCTAGAAGGGTG+AGG + chr5.2:22778457-22778476 MS.gene027737:CDS 40.0%
! GACACTGAATGAGTTCTAAC+AGG - chr5.2:22780037-22780056 None:intergenic 40.0%
! GAGGATAATTGGAGTGTTTC+TGG + chr5.2:22780649-22780668 MS.gene027737:CDS 40.0%
! TAGTCATTCTAGAAGGGTGA+GGG + chr5.2:22778458-22778477 MS.gene027737:CDS 40.0%
! TTGTGTAGGAAGAGCACAAA+TGG - chr5.2:22780797-22780816 None:intergenic 40.0%
!! CTACAAAAGCGTTGCATTTG+TGG + chr5.2:22778349-22778368 MS.gene027737:CDS 40.0%
!! GATGTACTGACTATGCGAAT+AGG + chr5.2:22780264-22780283 MS.gene027737:intron 40.0%
!! GTCAGGCTAAAATTTCTGGT+GGG - chr5.2:22779687-22779706 None:intergenic 40.0%
ACAAACTCACCTTTGTAGGG+TGG - chr5.2:22778434-22778453 None:intergenic 45.0%
ACTTGTGGATAGGTACCTTC+AGG + chr5.2:22779172-22779191 MS.gene027737:CDS 45.0%
AGCAATCAAATCTGGTGGCA+AGG + chr5.2:22778593-22778612 MS.gene027737:CDS 45.0%
ATTCTTGCATCCTCCATGGT+TGG + chr5.2:22778301-22778320 MS.gene027737:CDS 45.0%
ATTGAACATGGTAGCTCGGT+TGG - chr5.2:22780070-22780089 None:intergenic 45.0%
CATAAGCATTTGGACAGCAG+GGG - chr5.2:22778045-22778064 None:intergenic 45.0%
CATCTCTTGCAACTTCCTGA+AGG - chr5.2:22779190-22779209 None:intergenic 45.0%
CATTCACGCAGTCAGTACAT+TGG + chr5.2:22780169-22780188 MS.gene027737:intron 45.0%
CATTTGTGGTCCGAAGACTT+GGG + chr5.2:22778363-22778382 MS.gene027737:CDS 45.0%
GCATAAGCATTTGGACAGCA+GGG - chr5.2:22778046-22778065 None:intergenic 45.0%
GCTGGTAATTCCCAAGTCTT+CGG - chr5.2:22778376-22778395 None:intergenic 45.0%
GTAGCTCAGTGATACGAGTT+GGG + chr5.2:22779398-22779417 MS.gene027737:intron 45.0%
GTCAAGATAAGGAGTCAGTC+AGG - chr5.2:22779704-22779723 None:intergenic 45.0%
GTCTGAGTCTAACTCAGACT+CGG + chr5.2:22778719-22778738 MS.gene027737:CDS 45.0%
GTCTGAGTTAGACTCAGACT+CGG - chr5.2:22778718-22778737 None:intergenic 45.0%
TGTAGCTCAGTGATACGAGT+TGG + chr5.2:22779397-22779416 MS.gene027737:intron 45.0%
! ACAAGAAACGGCGCCTTTTT+CGG - chr5.2:22778805-22778824 None:intergenic 45.0%
! AGCTTGCTTCCAACCGAAAA+AGG + chr5.2:22778789-22778808 MS.gene027737:CDS 45.0%
! GTCCAACTTCCTGATTTTCC+GGG + chr5.2:22778117-22778136 MS.gene027737:CDS 45.0%
! TGAGTTCTAACAGGAGGTTC+TGG - chr5.2:22780028-22780047 None:intergenic 45.0%
! TGGTGATGAGATGAATCAGG+AGG - chr5.2:22778093-22778112 None:intergenic 45.0%
!!! ATGTTTTTTTATTTTTATTT+TGG + chr5.2:22779953-22779972 MS.gene027737:intron 5.0%
AGTCTTGAGCCACCCTACAA+AGG + chr5.2:22778422-22778441 MS.gene027737:CDS 50.0%
CAAGCTAGAGCAGCATCATC+TGG + chr5.2:22780514-22780533 MS.gene027737:CDS 50.0%
CACCCGGAAAATCAGGAAGT+TGG - chr5.2:22778122-22778141 None:intergenic 50.0%
CTATGGTGTCCTTCCAACCA+TGG - chr5.2:22778317-22778336 None:intergenic 50.0%
GCATTTGTGGTCCGAAGACT+TGG + chr5.2:22778362-22778381 MS.gene027737:CDS 50.0%
GGCATAAGCATTTGGACAGC+AGG - chr5.2:22778047-22778066 None:intergenic 50.0%
GTCAGACACTTACACGACAC+CGG + chr5.2:22777833-22777852 MS.gene027737:intron 50.0%
TCAAATGCTTCCACTCCACC+CGG - chr5.2:22778138-22778157 None:intergenic 50.0%
TTGCATCCTCCATGGTTGGA+AGG + chr5.2:22778305-22778324 MS.gene027737:CDS 50.0%
! AACGATCTTGGAGGGCTAGT+TGG - chr5.2:22779041-22779060 None:intergenic 50.0%
! AATGGAAGCGTGCATGCATG+CGG + chr5.2:22780438-22780457 MS.gene027737:CDS 50.0%
! ACATGGTAGCTCGGTTGGTA+AGG - chr5.2:22780065-22780084 None:intergenic 50.0%
! CAACTTCCTGATTTTCCGGG+TGG + chr5.2:22778120-22778139 MS.gene027737:CDS 50.0%
!! GTACTGACTGCGTGAATGTG+AGG - chr5.2:22780167-22780186 None:intergenic 50.0%
GCTAGAGCAGCATCATCTGG+TGG + chr5.2:22780517-22780536 MS.gene027737:CDS 55.0%
TCCACTCCACCCGGAAAATC+AGG - chr5.2:22778129-22778148 None:intergenic 55.0%
TGAAAGAAGGCGGTCGCGTT+CGG + chr5.2:22779070-22779089 MS.gene027737:CDS 55.0%
TGGTGTCCTTCCAACCATGG+AGG - chr5.2:22778314-22778333 None:intergenic 55.0%
! TCCTGATTTTCCGGGTGGAG+TGG + chr5.2:22778125-22778144 MS.gene027737:CDS 55.0%
!! GATCTTGGAGGGCTAGTTGG+TGG - chr5.2:22779038-22779057 None:intergenic 55.0%
!!! GAAACGGCGCCTTTTTCGGT+TGG - chr5.2:22778801-22778820 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 22777497 22780876 22777497 ID=MS.gene027737
chr5.2 mRNA 22777497 22780876 22777497 ID=MS.gene027737.t1;Parent=MS.gene027737
chr5.2 exon 22777497 22777555 22777497 ID=MS.gene027737.t1.exon1;Parent=MS.gene027737.t1
chr5.2 CDS 22777497 22777555 22777497 ID=cds.MS.gene027737.t1;Parent=MS.gene027737.t1
chr5.2 exon 22777660 22777729 22777660 ID=MS.gene027737.t1.exon2;Parent=MS.gene027737.t1
chr5.2 CDS 22777660 22777729 22777660 ID=cds.MS.gene027737.t1;Parent=MS.gene027737.t1
chr5.2 exon 22778039 22779313 22778039 ID=MS.gene027737.t1.exon3;Parent=MS.gene027737.t1
chr5.2 CDS 22778039 22779313 22778039 ID=cds.MS.gene027737.t1;Parent=MS.gene027737.t1
chr5.2 exon 22780370 22780876 22780370 ID=MS.gene027737.t1.exon4;Parent=MS.gene027737.t1
chr5.2 CDS 22780370 22780876 22780370 ID=cds.MS.gene027737.t1;Parent=MS.gene027737.t1
Gene Sequence

>MS.gene027737

ATGAAGTTTATGAAACTTGGATCTCGTCCAGATACTTTCTACACTTCAGAAGCTATAAGGTAAGACATTAACAAAAATAAATATCATCTTTGTTCTTTGATGATGCATCTCCTTTTTGGATTGTTTAATTTTGTAACATGAGTTCTTTTCTTTGTATATGCAGGACAATCTCTTCTGAAGTTTCTAGTGATCTCATAATTCAAGTAAGGGGAAGTAGATATCTTCTTCACAAGGTATGTTTCTTGAAAGTAGAATTAACTCATCAAGTTTCTTTTATTTATTTATTGAATTGTATATTGCTATGTAGCACAGACACTTCAAATTGAAGGCTTCTCAGTCAGACACTTACACGACACCGGCACGTATTGTTACATTTCGGAAGGAAAAGAAATAAAAAAAATTAGTATCCGTATCAATGTGTCATATTAGTGTTGTCTCTGGTGTTCGTGTTTGTGTTTGTGTCTGTGTCTGTGTCGCTTCTTCATAAGTATATAATTTTTTACTTTGGTAACTTATCTGAAGTTCATGCTCATGTTTTTCAGTTTCCCCTGCTGTCCAAATGCTTATGCCTGCAAAGATTATGCTCAGAATCACCTCCTGATTCATCTCATCACCAAATAGTCCAACTTCCTGATTTTCCGGGTGGAGTGGAAGCATTTGAGTTGTGTGCTAAGTTCTGCTATGGAATCACAATTACTTTAAGTCCTTACAACATTGTAGCTGCAAGATGTGCTGCTGAATATCTCCAAATGACTGAAGAAGTTGAAAAAGGAAACTTGATTCACAAGCTTGAGGTTTTTTTCAATTCTTGCATCCTCCATGGTTGGAAGGACACCATAGTAAGTCTTCAGACTACAAAAGCGTTGCATTTGTGGTCCGAAGACTTGGGAATTACCAGCAGATGCATTGAAACTATTGCGTCAAAAGTCTTGAGCCACCCTACAAAGGTGAGTTTGTCACATAGTCATTCTAGAAGGGTGAGGGATGACATATCTTGCAATGATACAGAAAGTTTGAGAATCAAATCAGGAAGTAAAGGATGGTGGGCAGAAGATTTAGCAGAACTTAGCATAGACCTTTATTGGAGAACAATGATAGCAATCAAATCTGGTGGCAAGGTTCCTTCAAATCTCATTGGTGATGCATTGAAAATTTATGCATCTAGATGGTTACCAAATATTTCCAAGAACGGAAATAGAAAGAAGAATGTTTCACTAGCCGAGTCTGAGTCTAACTCAGACTCGGCCAGCGAAATAACTTCAAAGCATAGGCTTCTATTGGAGTCGATAGTAAGCTTGCTTCCAACCGAAAAAGGCGCCGTTTCTTGTAGCTTTCTTTTGAAACTATTGAAAGCATCCAATATTCTCAATGCTTCTTCATCTTCAAAGATGGAATTAGCAAGAAGAGTAGGACTTCAATTGGAGGAAGCCACAGTGAATGATCTGTTAATACCTTCACTTTCTTATACAAATGATACAGTCTATGATGTTGAGTTGGTGATGACTATATTGGAACATTTCATGTTACAAGGACAAAGTCCACCAACTAGCCCTCCAAGATCGTTGAAGACTTTTGAAAGAAGGCGGTCGCGTTCGGCTGAGAATATTAACTTTGAATTACAAGAGAGTAGGAGGTCCTCTTCAGCATCACATAGTTCAAAATTGAAAGTGGCAAAACTTGTGGATAGGTACCTTCAGGAAGTTGCAAGAGATGTGAATTTTTCATTGTCAAAATTCATTGCTCTTGCTGATATTATACCAGAATTTGCAAGATATGATCATGATGACCTCTATAGAGCCATTGACATTTATCTCAAGGTAAAAATCATTCCTATAAAATGTTTATTGTATTTTTTTAAGTCACTCCATTTTATAACTTGGTAAAAATATATCTCAGATGGTGTAGCTCAGTGATACGAGTTGGGACCCTCAACGAGTATTAAGAAAGTGGCTCCGTGCTTAATCCCTTCTATCAACAATTTCCCCTTCTCGTAACGAACTACTAATCACTAACATTTGCCTATAAAAAAATACACACATAAAAAACATGTAAAAAAAAAGTGGCTGTACATAATTCATACTTCACTTTCAAATTTTAGGATATGAAACAGAATTAGTGGATAACCAACTCTCTTGACCTTAAAATATTGTCTTATTCAACATTTATTGGAAGTTAGGTGTGGACTGACCCACCAGAAATTTTAGCCTGACTGACTCCTTATCTTGACTGATATAGTTCCAGTAACATGTTCAGAAAATTTCATAACTCATTTGGCTAAAAACGTGAAGAAAAATTTCTTGTCAAAACTGTTACAGAGTTCCATCACAGTTCCAGTACTATGTCCTTCTCATGAATTGATAGTGTGTCTCTTCAATTTTACATCACAAGTTTTGTCCATTTATGAATTCACTCTATTTAGCCGATTTCCACCTCAATGAACTCTATAATGATGTAACATGTTTTTTTATTTTTATTTTGGCTATACAAATCAGTAAATTTTAATTTAAGTTTTTTTTCAAGGATTGAATCCAGAACCTCCTGTTAGAACTCATTCAGTGTCTCTTACCTTACCAACCGAGCTACCATGTTCAATTAATTAGGCTATGGAAGAAAAATTTATAGCTTGAGGCGTTATTGTAATGATTGGACTTACTCTTGTCACAAATTCCTCACATTCACGCAGTCAGTACATTGGTAGCTGTTAGATCAAAATTCGACGGTCCATATTTCAAGCTCGTTATTTTAGAGTTCAGAAAATTTCTTAACAGATGTACTGACTATGCGAATAGGTGAATATGTGACTACTGGCTGCAGCAACAAATTTATTATTATTCTTGCTGAAATTTATTTATTTCTTAATTTGAAACATGCAGGCTCATCCAGAACTCAACAAGAGTGAAAGGAAAAGACTATGTAGAATTCTTGACTGCAAAAAGCTCTCAATGGAAGCGTGCATGCATGCGGCGCAAAATGAGCTACTCCCTTTAAGAGTTGTTGTTCAAGTTCTGTTCTTTGAGCAAGCTAGAGCAGCATCATCTGGTGGCAAAGTAACAGAATTACAAAGCAATATCAAAGCATTGCTTACAACACATGGTATTGATCCATCAAAACATACAACAACACAGTTAAGCTCTACTACAAGCATTCAAGGTGAGGATAATTGGAGTGTTTCTGGTTTCAAGTCACCAAAATCAAAGTCTTCAACTCTAAGAATGAAGCTAGCTGAGGATCATGATGAATTTGATGAAAATGGTTTGGTAAATAATGATGGAATTGGAAGAAACTCTAAATTTAAGTCCATTTGTGCTCTTCCTACACAACCTAAAAAAATGCTTAGTAAATTGTGGTCTACTAATAGAACTGCTACTGAAAAAAATTGA
Protein sequence

>MS.gene027737.t1

MKFMKLGSRPDTFYTSEAIRTISSEVSSDLIIQVRGSRYLLHKFPLLSKCLCLQRLCSESPPDSSHHQIVQLPDFPGGVEAFELCAKFCYGITITLSPYNIVAARCAAEYLQMTEEVEKGNLIHKLEVFFNSCILHGWKDTIVSLQTTKALHLWSEDLGITSRCIETIASKVLSHPTKVSLSHSHSRRVRDDISCNDTESLRIKSGSKGWWAEDLAELSIDLYWRTMIAIKSGGKVPSNLIGDALKIYASRWLPNISKNGNRKKNVSLAESESNSDSASEITSKHRLLLESIVSLLPTEKGAVSCSFLLKLLKASNILNASSSSKMELARRVGLQLEEATVNDLLIPSLSYTNDTVYDVELVMTILEHFMLQGQSPPTSPPRSLKTFERRRSRSAENINFELQESRRSSSASHSSKLKVAKLVDRYLQEVARDVNFSLSKFIALADIIPEFARYDHDDLYRAIDIYLKAHPELNKSERKRLCRILDCKKLSMEACMHAAQNELLPLRVVVQVLFFEQARAASSGGKVTELQSNIKALLTTHGIDPSKHTTTQLSSTTSIQGEDNWSVSGFKSPKSKSSTLRMKLAEDHDEFDENGLVNNDGIGRNSKFKSICALPTQPKKMLSKLWSTNRTATEKN