AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene028062

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene028062.t1 MTR_5g026930 99.164 598 5 0 1 598 1 598 0.0 1241
MS.gene028062.t1 MTR_5g026930 99.198 499 4 0 1 499 1 499 0.0 1033
MS.gene028062.t1 MTR_8g071280 76.167 600 137 3 1 598 1 596 0.0 956
MS.gene028062.t1 MTR_1g101370 47.049 593 286 12 24 590 27 617 0.0 547
MS.gene028062.t1 MTR_0019s0120 49.146 527 255 8 72 590 105 626 1.17e-180 525
MS.gene028062.t1 MTR_8g432680 48.295 528 262 9 72 590 105 630 2.76e-179 521
MS.gene028062.t1 MTR_8g100075 46.958 526 269 7 72 590 98 620 1.81e-178 519
MS.gene028062.t1 MTR_7g076170 43.780 635 300 16 11 598 12 636 9.32e-175 510
MS.gene028062.t1 MTR_8g055840 42.833 600 313 10 13 590 9 600 1.58e-167 491
MS.gene028062.t1 MTR_5g040360 42.000 600 317 10 13 590 9 599 6.74e-167 489
MS.gene028062.t1 MTR_4g098890 44.351 593 300 11 20 589 17 602 1.40e-164 510
MS.gene028062.t1 MTR_7g006060 46.332 518 268 7 57 566 28 543 8.13e-164 479
MS.gene028062.t1 MTR_3g040370 43.072 599 309 14 13 590 10 597 1.18e-163 481
MS.gene028062.t1 MTR_7g076150 44.278 533 267 10 71 591 74 588 2.02e-154 456
MS.gene028062.t1 MTR_5g080720 43.036 560 294 11 38 587 93 637 1.95e-153 456
MS.gene028062.t1 MTR_8g432620 40.721 555 300 9 49 590 62 600 2.55e-151 449
MS.gene028062.t1 MTR_1g442840 41.530 549 286 8 67 595 87 620 1.95e-150 447
MS.gene028062.t1 MTR_0019s0120 49.425 435 208 7 72 499 105 534 9.20e-148 437
MS.gene028062.t1 MTR_3g068990 43.566 544 279 11 60 589 138 667 9.48e-148 442
MS.gene028062.t1 MTR_1g442840 43.056 504 266 6 103 595 14 507 1.76e-147 436
MS.gene028062.t1 MTR_1g069785 40.331 605 328 12 6 595 4 590 3.37e-147 438
MS.gene028062.t1 MTR_1g075900 40.727 550 291 9 66 595 85 619 2.39e-144 432
MS.gene028062.t1 MTR_5g021100 41.573 534 273 13 81 597 329 840 3.89e-142 433
MS.gene028062.t1 MTR_8g076650 43.074 527 266 11 81 593 387 893 2.03e-140 430
MS.gene028062.t1 MTR_1g029730 42.366 524 271 14 81 589 151 658 9.37e-139 418
MS.gene028062.t1 MTR_3g096480 42.135 534 270 14 81 595 268 781 5.11e-138 421
MS.gene028062.t1 MTR_5g083150 40.789 532 273 12 81 597 101 605 1.30e-136 411
MS.gene028062.t1 MTR_7g076150 44.924 463 225 10 141 591 1 445 4.85e-134 399
MS.gene028062.t1 MTR_5g080720 42.656 497 261 10 38 525 93 574 5.46e-134 404
MS.gene028062.t1 MTR_3g088790 41.651 533 271 15 81 593 233 745 3.49e-132 404
MS.gene028062.t1 MTR_8g093880 39.785 558 296 14 53 593 275 809 6.00e-132 406
MS.gene028062.t1 MTR_3g071530 41.300 523 269 12 81 592 153 648 1.95e-131 399
MS.gene028062.t1 MTR_1g051055 38.436 601 315 15 25 590 23 603 9.12e-126 383
MS.gene028062.t1 MTR_1g022255 36.832 524 290 13 80 592 208 701 1.10e-110 347
MS.gene028062.t1 MTR_5g064080 32.541 547 315 14 75 591 149 671 4.64e-96 308
MS.gene028062.t1 MTR_3g074780 31.424 576 344 15 51 591 130 689 1.31e-93 302
MS.gene028062.t1 MTR_4g083030 32.143 560 324 14 55 587 71 601 5.62e-91 293
MS.gene028062.t1 MTR_1g022255 32.481 391 227 11 80 460 208 571 1.76e-60 211
MS.gene028062.t1 MTR_5g033760 63.636 66 24 0 533 598 41 106 2.95e-23 95.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene028062.t1 AT1G31850 73.588 602 151 4 4 597 2 603 0.0 956
MS.gene028062.t1 AT1G31850 73.588 602 151 4 4 597 2 603 0.0 956
MS.gene028062.t1 AT1G31850 73.588 602 151 4 4 597 2 603 0.0 956
MS.gene028062.t1 AT1G31850 73.588 602 151 4 4 597 2 603 0.0 956
MS.gene028062.t1 AT4G19120 74.000 600 152 3 1 598 1 598 0.0 954
MS.gene028062.t1 AT4G19120 74.000 600 152 3 1 598 1 598 0.0 954
MS.gene028062.t1 AT4G19120 74.251 501 125 3 1 499 1 499 0.0 796
MS.gene028062.t1 AT4G10440 46.805 532 272 7 72 594 93 622 5.43e-179 521
MS.gene028062.t1 AT1G33170 46.429 532 275 7 72 595 110 639 1.34e-176 515
MS.gene028062.t1 AT4G00750 42.599 608 315 11 14 590 18 622 2.65e-174 509
MS.gene028062.t1 AT2G45750 46.704 531 271 7 71 590 86 615 5.47e-173 505
MS.gene028062.t1 AT4G18030 43.464 612 314 11 10 596 5 609 1.07e-170 499
MS.gene028062.t1 AT1G26850 41.735 611 323 13 14 597 11 615 2.75e-165 485
MS.gene028062.t1 AT1G26850 41.735 611 323 13 14 597 11 615 2.75e-165 485
MS.gene028062.t1 AT2G43200 46.493 499 260 7 72 566 91 586 2.20e-162 478
MS.gene028062.t1 AT2G43200 46.493 499 260 7 72 566 91 586 2.20e-162 478
MS.gene028062.t1 AT4G00740 43.094 543 276 11 66 595 77 599 4.52e-158 466
MS.gene028062.t1 AT4G00740 43.094 543 276 11 66 595 77 599 4.52e-158 466
MS.gene028062.t1 AT2G39750 43.750 544 279 11 57 589 164 691 6.76e-150 448
MS.gene028062.t1 AT5G06050 41.758 546 289 11 56 589 135 663 7.83e-146 437
MS.gene028062.t1 AT3G23300 41.774 541 291 8 69 595 76 606 1.35e-145 434
MS.gene028062.t1 AT3G23300 41.774 541 291 8 69 595 76 606 1.35e-145 434
MS.gene028062.t1 AT2G34300 43.609 532 264 12 81 595 250 762 2.28e-144 436
MS.gene028062.t1 AT2G34300 43.609 532 264 12 81 595 250 762 2.28e-144 436
MS.gene028062.t1 AT2G34300 43.609 532 264 12 81 595 250 762 2.28e-144 436
MS.gene028062.t1 AT1G04430 40.753 584 308 13 34 595 51 618 2.74e-144 431
MS.gene028062.t1 AT1G04430 40.753 584 308 13 34 595 51 618 2.74e-144 431
MS.gene028062.t1 AT1G04430 40.753 584 308 13 34 595 51 618 2.74e-144 431
MS.gene028062.t1 AT4G14360 41.590 541 292 8 69 595 73 603 2.70e-142 426
MS.gene028062.t1 AT4G14360 41.590 541 292 8 69 595 73 603 2.70e-142 426
MS.gene028062.t1 AT1G77260 41.113 557 291 13 54 589 115 655 2.51e-141 425
MS.gene028062.t1 AT1G29470 42.857 532 268 13 81 595 250 762 1.50e-140 427
MS.gene028062.t1 AT1G29470 42.857 532 268 13 81 595 250 762 1.50e-140 427
MS.gene028062.t1 AT5G64030 43.100 529 268 11 81 593 308 819 7.76e-140 427
MS.gene028062.t1 AT2G40280 41.877 554 267 16 64 598 72 589 2.92e-137 412
MS.gene028062.t1 AT3G56080 43.238 525 257 14 81 592 114 610 4.00e-137 412
MS.gene028062.t1 AT5G14430 39.032 620 334 14 1 591 1 605 6.13e-137 412
MS.gene028062.t1 AT3G51070 41.308 535 273 12 81 595 381 894 1.58e-135 417
MS.gene028062.t1 AT5G14430 38.548 620 337 14 1 591 1 605 1.01e-134 407
MS.gene028062.t1 AT3G10200 36.975 595 332 14 19 590 14 588 1.38e-129 392
MS.gene028062.t1 AT1G26850 41.189 488 256 11 14 476 11 492 5.77e-127 383
MS.gene028062.t1 AT5G04060 38.374 529 296 9 75 590 86 597 5.35e-124 379
MS.gene028062.t1 AT3G56080 45.197 458 217 13 81 525 114 550 1.53e-123 375
MS.gene028062.t1 AT3G10200 35.849 583 324 14 19 571 14 576 1.20e-120 370
MS.gene028062.t1 AT3G51070 42.174 460 228 11 81 520 381 822 9.83e-118 369
MS.gene028062.t1 AT1G19430 36.243 527 290 15 80 592 230 724 1.62e-109 345
MS.gene028062.t1 AT1G19430 36.243 527 290 15 80 592 230 724 1.62e-109 345
MS.gene028062.t1 AT1G78240 30.293 614 364 16 16 587 85 676 5.93e-94 303
MS.gene028062.t1 AT1G78240 30.293 614 364 16 16 587 85 676 5.93e-94 303
MS.gene028062.t1 AT1G13860 31.588 554 313 11 72 591 81 602 4.95e-92 296
MS.gene028062.t1 AT1G13860 31.588 554 313 11 72 591 81 602 4.95e-92 296
MS.gene028062.t1 AT1G13860 31.588 554 313 11 72 591 81 602 4.95e-92 296
MS.gene028062.t1 AT1G13860 31.588 554 313 11 72 591 81 602 4.95e-92 296
MS.gene028062.t1 AT2G03480 32.092 564 316 14 52 587 66 590 1.24e-84 276
MS.gene028062.t1 AT2G03480 32.270 564 326 15 52 587 66 601 4.93e-84 275
MS.gene028062.t1 AT1G13860 31.828 465 265 9 156 589 1 444 1.51e-77 253
MS.gene028062.t1 AT1G19430 33.924 395 219 13 80 461 230 595 6.07e-64 221
MS.gene028062.t1 AT2G03480 30.882 340 217 7 52 382 66 396 1.60e-44 164

Find 149 sgRNAs with CRISPR-Local

Find 210 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTTCGTCCCGGAGGATTT+TGG 0.137518 5.3:+16725124 MS.gene028062:CDS
ATGAATGTTGTTTCATCTTA+TGG 0.183938 5.3:+16725687 MS.gene028062:CDS
CTTATCCCATGGACAGAATT+TGG 0.187513 5.3:+16724852 MS.gene028062:CDS
TGAACAAGTGAAATCCTTTC+AGG 0.225038 5.3:-16725492 None:intergenic
TCAAGACTGTCATCACATTT+TGG 0.235686 5.3:-16725354 None:intergenic
TGATAATCATTGCTGCATTC+AGG 0.238492 5.3:-16723884 None:intergenic
CCCTTCAAACTCCTTTGATA+TGG 0.267872 5.3:+16724812 MS.gene028062:CDS
GGAGAGAAATTTCAATTTCC+TGG 0.292268 5.3:+16724378 MS.gene028062:CDS
GAAGAATTCGGTGAATTTCT+TGG 0.297915 5.3:-16725107 None:intergenic
CTTGGTGGAGTCTTTAAATC+TGG 0.314276 5.3:+16723767 MS.gene028062:CDS
CTTCAAATTATACAACAAAA+AGG 0.343104 5.3:+16725260 MS.gene028062:CDS
CAACATTCATCTCTACTCTT+TGG 0.346033 5.3:-16724166 None:intergenic
ACTGACCCATCTTTGATTCC+TGG 0.346142 5.3:-16724462 None:intergenic
AAAACTACAGGGAGTGTATT+AGG 0.348105 5.3:-16725711 None:intergenic
TTCAACCTTTGAGGCCATTT+CGG 0.348599 5.3:-16725462 None:intergenic
ATCATAGCAAGCGTTGTCTT+TGG 0.357055 5.3:-16725307 None:intergenic
CCTTGATCAATGATCCTTTG+TGG 0.361183 5.3:+16725661 MS.gene028062:CDS
AAGAAGTTGTTGCCAGATCT+TGG 0.367212 5.3:+16725579 MS.gene028062:CDS
CTACTCTTTGGCCATCTGAT+TGG 0.368686 5.3:-16724154 None:intergenic
CTTGATCAATGATCCTTTGT+GGG 0.371015 5.3:+16725662 MS.gene028062:CDS
ATCTTGTTTGTGCCAAGATC+TGG 0.373912 5.3:-16725591 None:intergenic
CACAAAGAAAACACTGAGTT+TGG 0.376578 5.3:+16726560 MS.gene028062:CDS
CAGTCAACTATGAGCGTAGA+TGG 0.383909 5.3:+16725163 MS.gene028062:CDS
CTCTTTGGCCATCTGATTGG+AGG 0.385460 5.3:-16724151 None:intergenic
TTACAGAGATGGAGAAAGTA+TGG 0.391225 5.3:+16724040 MS.gene028062:intron
CGCGGTATCCCAGCAATTCT+TGG 0.393361 5.3:+16724762 MS.gene028062:CDS
TAATCAGCATTGGTTGATAA+AGG 0.396465 5.3:+16724353 MS.gene028062:CDS
GCCCGCCTGGTCGCAAAATC+CGG 0.400319 5.3:-16726463 None:intergenic
ATGGATATGAATACAGCTTA+TGG 0.401394 5.3:+16725624 MS.gene028062:CDS
CAAACTGAACCTGAGCTTCA+TGG 0.406657 5.3:-16724731 None:intergenic
ACGTATTCACCAACACCATT+AGG 0.407310 5.3:-16724417 None:intergenic
ATTTATGTTAGGTTGCTAGC+TGG 0.410778 5.3:+16724652 MS.gene028062:intron
ATCCTCCGGGACGAAGAATT+CGG 0.410892 5.3:-16725119 None:intergenic
GTTAGAACTGCAATTGATAC+TGG 0.412772 5.3:+16724483 MS.gene028062:CDS
TGTTTGTTAATCCAATCATA+TGG 0.415030 5.3:-16724324 None:intergenic
TTTATGTTAGGTTGCTAGCT+GGG 0.415622 5.3:+16724653 MS.gene028062:intron
TACCTATAGACTTACTTTGT+TGG 0.417092 5.3:+16724063 MS.gene028062:CDS
CGGATTATAGCGTGCCCGCC+TGG 0.419332 5.3:-16726476 None:intergenic
TAGCTGGGGCGGTGACTTGT+TGG 0.424219 5.3:+16724668 MS.gene028062:CDS
GAGATGACACCAAGAATTGC+TGG 0.425210 5.3:-16724771 None:intergenic
CAAATTCTGTCCATGGGATA+AGG 0.432198 5.3:-16724851 None:intergenic
CTGCATTCAGGGAAACTAAA+GGG 0.432642 5.3:-16723872 None:intergenic
GAAAGGATTTCACTTGTTCA+AGG 0.433009 5.3:+16725495 MS.gene028062:CDS
ATGGAAAAGTACAAGAAATC+AGG 0.433649 5.3:+16725426 MS.gene028062:CDS
GAAGTATGTAATGTTGGAAA+TGG 0.436291 5.3:+16726439 MS.gene028062:CDS
GATAATCATTGCTGCATTCA+GGG 0.443927 5.3:-16723883 None:intergenic
CTGACCCATCTTTGATTCCT+GGG 0.449263 5.3:-16724461 None:intergenic
GGAGTGTACCATGCTGAATC+TGG 0.456691 5.3:-16725378 None:intergenic
GTCAGAGATAGGTTCTTGTT+CGG 0.459074 5.3:-16723701 None:intergenic
TGATCGCCGCAGTCAGAGAT+AGG 0.460105 5.3:-16723712 None:intergenic
TGAAGTAGGTCATAGGTTCT+AGG 0.468060 5.3:-16725906 None:intergenic
ACAGGAATGTGCCATATGAT+TGG 0.476125 5.3:+16724313 MS.gene028062:intron
AGGCCTCGATCGAAAACTAC+AGG 0.476192 5.3:-16725723 None:intergenic
GGTGCAACATTCAACCTTTG+AGG 0.476749 5.3:-16725471 None:intergenic
GAGATAGGTTCTTGTTCGGC+TGG 0.478220 5.3:-16723697 None:intergenic
AACAAGGATCATGAAGCATA+AGG 0.478806 5.3:+16723658 None:intergenic
TGAGCTTCATGGTTATCTCT+TGG 0.482556 5.3:-16724720 None:intergenic
GCCTCACGTACATACCGAAA+TGG 0.482618 5.3:+16725448 MS.gene028062:CDS
TGAAATGAAGTATGTAATGT+TGG 0.482893 5.3:+16726433 MS.gene028062:CDS
GAATTACCAAATTCTGTCCA+TGG 0.489900 5.3:-16724858 None:intergenic
CTCATAGTTGACTGGTGGAC+CGG 0.491832 5.3:-16725154 None:intergenic
CATCTTGATCCTGAGTTGGA+TGG 0.492062 5.3:-16726629 None:intergenic
AAATTTCAATTTCCTGGTGG+AGG 0.497318 5.3:+16724384 MS.gene028062:CDS
CTGATCCCAGGAATCAAAGA+TGG 0.497573 5.3:+16724456 MS.gene028062:CDS
AAAAGGATGACATATATGTA+TGG 0.500938 5.3:+16725277 MS.gene028062:CDS
TTCCAACAAAGTAAGTCTAT+AGG 0.502688 5.3:-16724065 None:intergenic
ATCAGCATGAAGTAGGTCAT+AGG 0.503060 5.3:-16725913 None:intergenic
TGCTTAGTTCCTCCTCCACC+TGG 0.503617 5.3:+16724121 MS.gene028062:CDS
TGACATATATGTATGGCAAA+AGG 0.507120 5.3:+16725284 MS.gene028062:CDS
CTGATTGGAGGTTTGTAACC+AGG 0.512073 5.3:-16724139 None:intergenic
AAAGAGCCTGAGGATTGCTT+AGG 0.516610 5.3:-16723839 None:intergenic
ATAGGTTCTTGTTCGGCTGG+TGG 0.517533 5.3:-16723694 None:intergenic
GTAGAGATGAATGTTGGTAC+AGG 0.518059 5.3:+16724173 MS.gene028062:CDS
TTATCGGTACCTTCCATGAC+TGG 0.519417 5.3:+16725745 MS.gene028062:CDS
TCAGTCATGATAACAGTAAG+TGG 0.521028 5.3:+16725535 MS.gene028062:CDS
TTTGGAGGATAGGTGTCTCT+AGG 0.521324 5.3:-16725336 None:intergenic
GCCATATCAAAGGAGTTTGA+AGG 0.521561 5.3:-16724813 None:intergenic
CATCTACGCTCATAGTTGAC+TGG 0.523463 5.3:-16725162 None:intergenic
GGACAAAACCCAAAATCCTC+CGG 0.525700 5.3:-16725133 None:intergenic
GGAGGTACTATGTTTCCTAA+TGG 0.527366 5.3:+16724402 MS.gene028062:CDS
AAGAGATAACCATGAAGCTC+AGG 0.528994 5.3:+16724722 MS.gene028062:CDS
TTGTCATCTTGATCCTGAGT+TGG 0.530646 5.3:-16726633 None:intergenic
ATGAGCGTAGATGGCGCGGA+TGG 0.530662 5.3:+16725172 MS.gene028062:CDS
TCTTCACTGCTGAAAGCCAC+AGG 0.536372 5.3:+16725940 MS.gene028062:CDS
TATGACCTACTTCATGCTGA+TGG 0.537222 5.3:+16725915 MS.gene028062:CDS
GGTTGAATGTTGCACCTGAA+AGG 0.539137 5.3:+16725478 MS.gene028062:CDS
CCACAAAGGATCATTGATCA+AGG 0.542881 5.3:-16725661 None:intergenic
ATGTAATGTTGGAAATGGAC+CGG 0.544880 5.3:+16726444 MS.gene028062:CDS
AAACAAGATAAGAAATGTGA+TGG 0.544976 5.3:+16725605 MS.gene028062:CDS
GCTGCATTCAGGGAAACTAA+AGG 0.545484 5.3:-16723873 None:intergenic
AACAGAAGTCTAATCAGCAT+TGG 0.548252 5.3:+16724343 MS.gene028062:CDS
AGTTTCAAACATACCAGTCA+TGG 0.548567 5.3:-16725758 None:intergenic
TGCCATCGCAACTATTGCGA+AGG 0.549327 5.3:+16726520 MS.gene028062:CDS
TCAAAGGAGTTTGAAGGGAA+AGG 0.552291 5.3:-16724807 None:intergenic
GTCTTTAAATCTGGAAACAA+TGG 0.552619 5.3:+16723776 MS.gene028062:CDS
ACCCTTCGCAATAGTTGCGA+TGG 0.553385 5.3:-16726522 None:intergenic
TTAATGCAAGATCTGATCCC+AGG 0.555758 5.3:+16724444 MS.gene028062:CDS
CAGCATTGGTTGATAAAGGA+AGG 0.557499 5.3:+16724357 MS.gene028062:CDS
CTTAATTTGCAAAGAGCCTG+AGG 0.559105 5.3:-16723849 None:intergenic
TTATGTTAGGTTGCTAGCTG+GGG 0.559993 5.3:+16724654 MS.gene028062:intron
GGTTTGTAACCAGGTGGAGG+AGG 0.568649 5.3:-16724130 None:intergenic
CCATATCAAAGGAGTTTGAA+GGG 0.568671 5.3:-16724812 None:intergenic
GATTCACCTAAGCAATCCTC+AGG 0.569880 5.3:+16723833 MS.gene028062:CDS
ATCACTATCATTGTTCTGTG+TGG 0.571055 5.3:+16723731 MS.gene028062:CDS
GAGAAATTTCAATTTCCTGG+TGG 0.571653 5.3:+16724381 MS.gene028062:CDS
GGAGGTTTGTAACCAGGTGG+AGG 0.571709 5.3:-16724133 None:intergenic
CCGCAAAATAACTTGACTCT+CGG 0.573098 5.3:-16726496 None:intergenic
CTGTGGCAGCCATCCAACTC+AGG 0.575238 5.3:+16726620 MS.gene028062:CDS
GACAAAACCCAAAATCCTCC+GGG 0.575836 5.3:-16725132 None:intergenic
TGTTAGGTTGCTAGCTGGGG+CGG 0.577359 5.3:+16724657 MS.gene028062:intron
AGGATCATGAAGCATAAGGA+TGG 0.579185 5.3:+16723662 None:intergenic
AATTACCAAATTCTGTCCAT+GGG 0.580974 5.3:-16724857 None:intergenic
AGTCATGGAAGGTACCGATA+AGG 0.582392 5.3:-16725743 None:intergenic
AATACCTTTGGATCTGTGCA+TGG 0.582765 5.3:-16723917 None:intergenic
CTGCAATTGATACTGGTTGT+GGG 0.583160 5.3:+16724490 MS.gene028062:CDS
TACTCCATGCACAGATCCAA+AGG 0.583464 5.3:+16723913 MS.gene028062:CDS
ATTCACCGAATTCTTCGTCC+CGG 0.585958 5.3:+16725114 MS.gene028062:CDS
ACATACCGAAATGGCCTCAA+AGG 0.586199 5.3:+16725457 MS.gene028062:CDS
GCTCTAGATGCCTTATCCCA+TGG 0.586277 5.3:+16724841 MS.gene028062:CDS
CAAAGAGTAGAGATGAATGT+TGG 0.586343 5.3:+16724167 MS.gene028062:CDS
AGAATCCATCAGCATGAAGT+AGG 0.586900 5.3:-16725920 None:intergenic
AACTATGAGCGTAGATGGCG+CGG 0.587927 5.3:+16725168 MS.gene028062:CDS
CACCGAATTCTTCGTCCCGG+AGG 0.588572 5.3:+16725117 MS.gene028062:CDS
GCCATTTCGGTATGTACGTG+AGG 0.590634 5.3:-16725449 None:intergenic
ACTATGTTTCCTAATGGTGT+TGG 0.597032 5.3:+16724408 MS.gene028062:CDS
ACTGCAATTGATACTGGTTG+TGG 0.599923 5.3:+16724489 MS.gene028062:CDS
GGCGGTGACTTGTTGGATCG+AGG 0.603333 5.3:+16724675 MS.gene028062:CDS
AGATGACACCAAGAATTGCT+GGG 0.605182 5.3:-16724770 None:intergenic
TGATCCCAGGAATCAAAGAT+GGG 0.614158 5.3:+16724457 MS.gene028062:CDS
CTATTGCGAAGGGTATGAGA+TGG 0.620603 5.3:+16726531 MS.gene028062:CDS
CTACGCTCATAGTTGACTGG+TGG 0.622282 5.3:-16725159 None:intergenic
GTTACAAACCTCCAATCAGA+TGG 0.623877 5.3:+16724143 MS.gene028062:CDS
TGAATCAAGTGATTTCTGAA+TGG 0.624350 5.3:-16723816 None:intergenic
GTTCAGTTTGCTCTCGAGCG+CGG 0.634804 5.3:+16724744 MS.gene028062:CDS
GATATGAATACAGCTTATGG+AGG 0.639260 5.3:+16725627 MS.gene028062:CDS
GCCATCGCAACTATTGCGAA+GGG 0.648197 5.3:+16726521 MS.gene028062:CDS
ATTGGAGGTTTGTAACCAGG+TGG 0.648361 5.3:-16724136 None:intergenic
AGAGCAATGAGCCATATCAA+AGG 0.654623 5.3:-16724823 None:intergenic
ACAACATTCATAACCCACAA+AGG 0.657750 5.3:-16725675 None:intergenic
GTCTTGAACCAGATTCAGCA+TGG 0.660598 5.3:+16725370 MS.gene028062:CDS
TCAAACATACCAGTCATGGA+AGG 0.663945 5.3:-16725754 None:intergenic
GGCCTCGATCGAAAACTACA+GGG 0.664815 5.3:-16725722 None:intergenic
TGCAATTGATACTGGTTGTG+GGG 0.666739 5.3:+16724491 MS.gene028062:CDS
ACAACAAAACAAGCGCGAAG+CGG 0.670028 5.3:-16725399 None:intergenic
CAAGAACCTATCTCTGACTG+CGG 0.672277 5.3:+16723706 MS.gene028062:CDS
GGAAACATAGTACCTCCACC+AGG 0.698372 5.3:-16724396 None:intergenic
GCGCGGATGGAACACAACGG+TGG 0.706806 5.3:+16725185 MS.gene028062:CDS
CAGATTTAAAGACTCCACCA+AGG 0.721559 5.3:-16723766 None:intergenic
ATGGCGCGGATGGAACACAA+CGG 0.806331 5.3:+16725182 MS.gene028062:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTATTAATAATATAAACACA+AGG + chr5.3:16726023-16726042 MS.gene028062:intron 15.0%
!! TTAGTATTAATTAATACCTT+TGG - chr5.3:16723932-16723951 None:intergenic 15.0%
!!! AATAGTTTTCTACAAAAATT+TGG + chr5.3:16723950-16723969 MS.gene028062:intron 15.0%
!!! TTTGTATTTTTACTTATTAC+CGG + chr5.3:16724566-16724585 MS.gene028062:intron 15.0%
!! AAGAAGTAAAAGTAAAAAGA+AGG - chr5.3:16724527-16724546 None:intergenic 20.0%
!! AAGTAAAAATACAAAGAAAG+CGG - chr5.3:16724561-16724580 None:intergenic 20.0%
!! CTTCAAATTATACAACAAAA+AGG + chr5.3:16725260-16725279 MS.gene028062:CDS 20.0%
!! TATGTTATAAGTGCTTAATT+AGG + chr5.3:16726324-16726343 MS.gene028062:intron 20.0%
!! TGTTGAAAAAGAAAAGATAT+TGG + chr5.3:16726583-16726602 MS.gene028062:CDS 20.0%
!! TTGTTATAAGATAAGTCTAA+GGG + chr5.3:16726088-16726107 MS.gene028062:intron 20.0%
!!! CTTTTACTTCTTTTTACAAT+TGG + chr5.3:16724535-16724554 MS.gene028062:intron 20.0%
!!! TATTCATATCTTATGCTTTT+AGG + chr5.3:16725072-16725091 MS.gene028062:intron 20.0%
!!! TGTTTTTGTGTTATATCATA+TGG + chr5.3:16725815-16725834 MS.gene028062:intron 20.0%
!!! TTATATCATATGGTTTTCTT+TGG + chr5.3:16725825-16725844 MS.gene028062:intron 20.0%
!!! TTTGTTATAAGATAAGTCTA+AGG + chr5.3:16726087-16726106 MS.gene028062:intron 20.0%
! AAAACATAGCATAAACAGAA+AGG - chr5.3:16724929-16724948 None:intergenic 25.0%
! AAAAGGATGACATATATGTA+TGG + chr5.3:16725277-16725296 MS.gene028062:CDS 25.0%
! AAACAAGATAAGAAATGTGA+TGG + chr5.3:16725605-16725624 MS.gene028062:CDS 25.0%
! TCTACAAAAATTTGGATGTT+TGG + chr5.3:16723958-16723977 MS.gene028062:intron 25.0%
! TGAAATGAAGTATGTAATGT+TGG + chr5.3:16726433-16726452 MS.gene028062:CDS 25.0%
! TGTTATAAGATAAGTCTAAG+GGG + chr5.3:16726089-16726108 MS.gene028062:intron 25.0%
! TTCATAAACACATAACCTTA+TGG - chr5.3:16725795-16725814 None:intergenic 25.0%
!! AATTTTCATCCATTAACTGA+TGG + chr5.3:16725046-16725065 MS.gene028062:intron 25.0%
!! ATTTTCATCCATTAACTGAT+GGG + chr5.3:16725047-16725066 MS.gene028062:intron 25.0%
!! TGTTTGTTAATCCAATCATA+TGG - chr5.3:16724327-16724346 None:intergenic 25.0%
!!! ATGAATGTTGTTTCATCTTA+TGG + chr5.3:16725687-16725706 MS.gene028062:CDS 25.0%
!!! GCGTTTTGAAAATCTTATTT+AGG - chr5.3:16726368-16726387 None:intergenic 25.0%
!!! TTATGATGACTTGTTCTATT+AGG + chr5.3:16725862-16725881 MS.gene028062:intron 25.0%
AACTCTACATATGAACCATA+AGG + chr5.3:16725777-16725796 MS.gene028062:intron 30.0%
AATTACCAAATTCTGTCCAT+GGG - chr5.3:16724860-16724879 None:intergenic 30.0%
ACATAACTCCGATTTATGTT+AGG + chr5.3:16724641-16724660 MS.gene028062:intron 30.0%
ATGAAACTGTTTCATTATGC+TGG - chr5.3:16724963-16724982 None:intergenic 30.0%
ATGGATATGAATACAGCTTA+TGG + chr5.3:16725624-16725643 MS.gene028062:CDS 30.0%
CATTCTTTCCTCTCAAAAAT+CGG - chr5.3:16724103-16724122 None:intergenic 30.0%
GAAGTATGTAATGTTGGAAA+TGG + chr5.3:16726439-16726458 MS.gene028062:CDS 30.0%
GTCTTTAAATCTGGAAACAA+TGG + chr5.3:16723776-16723795 MS.gene028062:CDS 30.0%
GTGTTACATGAATAAATGAC+AGG + chr5.3:16724295-16724314 MS.gene028062:intron 30.0%
TAACATAAATCGGAGTTATG+TGG - chr5.3:16724642-16724661 None:intergenic 30.0%
TACCTATAGACTTACTTTGT+TGG + chr5.3:16724063-16724082 MS.gene028062:CDS 30.0%
TGAATATTCCCATCAGTTAA+TGG - chr5.3:16725058-16725077 None:intergenic 30.0%
TGAATCAAGTGATTTCTGAA+TGG - chr5.3:16723819-16723838 None:intergenic 30.0%
TGACATATATGTATGGCAAA+AGG + chr5.3:16725284-16725303 MS.gene028062:CDS 30.0%
TTCCAACAAAGTAAGTCTAT+AGG - chr5.3:16724068-16724087 None:intergenic 30.0%
! AATCTTATTTAGGCCAAGTT+TGG - chr5.3:16726358-16726377 None:intergenic 30.0%
! AATGGATGATTTTACAGAGA+TGG + chr5.3:16724029-16724048 MS.gene028062:intron 30.0%
! ATGGAAAAGTACAAGAAATC+AGG + chr5.3:16725426-16725445 MS.gene028062:CDS 30.0%
! GATTTCTTGTACTTTTCCAT+TGG - chr5.3:16725426-16725445 None:intergenic 30.0%
! TCATATCTTATGCTTTTAGG+TGG + chr5.3:16725075-16725094 MS.gene028062:intron 30.0%
! TTAGGTTGTTTATCCAAACT+TGG + chr5.3:16726342-16726361 MS.gene028062:intron 30.0%
!! TAATCAGCATTGGTTGATAA+AGG + chr5.3:16724353-16724372 MS.gene028062:CDS 30.0%
!!! TGTTGATTGAGTTTTGAGAA+TGG + chr5.3:16724011-16724030 MS.gene028062:intron 30.0%
AAATTTCAATTTCCTGGTGG+AGG + chr5.3:16724384-16724403 MS.gene028062:CDS 35.0%
AACAGAAGTCTAATCAGCAT+TGG + chr5.3:16724343-16724362 MS.gene028062:CDS 35.0%
ACAACATTCATAACCCACAA+AGG - chr5.3:16725678-16725697 None:intergenic 35.0%
ACTATGTTTCCTAATGGTGT+TGG + chr5.3:16724408-16724427 MS.gene028062:CDS 35.0%
AGTTTCAAACATACCAGTCA+TGG - chr5.3:16725761-16725780 None:intergenic 35.0%
ATCACTATCATTGTTCTGTG+TGG + chr5.3:16723731-16723750 MS.gene028062:CDS 35.0%
ATGTAATGTTGGAAATGGAC+CGG + chr5.3:16726444-16726463 MS.gene028062:CDS 35.0%
CAAAGAGTAGAGATGAATGT+TGG + chr5.3:16724167-16724186 MS.gene028062:CDS 35.0%
CAACATTCATCTCTACTCTT+TGG - chr5.3:16724169-16724188 None:intergenic 35.0%
CACAAAGAAAACACTGAGTT+TGG + chr5.3:16726560-16726579 MS.gene028062:CDS 35.0%
CCATATCAAAGGAGTTTGAA+GGG - chr5.3:16724815-16724834 None:intergenic 35.0%
CTTGATCAATGATCCTTTGT+GGG + chr5.3:16725662-16725681 MS.gene028062:CDS 35.0%
GAAAGGATTTCACTTGTTCA+AGG + chr5.3:16725495-16725514 MS.gene028062:CDS 35.0%
GAATTACCAAATTCTGTCCA+TGG - chr5.3:16724861-16724880 None:intergenic 35.0%
GAGAAATTTCAATTTCCTGG+TGG + chr5.3:16724381-16724400 MS.gene028062:CDS 35.0%
GATAATCATTGCTGCATTCA+GGG - chr5.3:16723886-16723905 None:intergenic 35.0%
GATATGAATACAGCTTATGG+AGG + chr5.3:16725627-16725646 MS.gene028062:CDS 35.0%
GCTAGCAACCTAACATAAAT+CGG - chr5.3:16724652-16724671 None:intergenic 35.0%
GCTTGTTTGAGCTTATCTAA+TGG + chr5.3:16726124-16726143 MS.gene028062:intron 35.0%
GGAGAGAAATTTCAATTTCC+TGG + chr5.3:16724378-16724397 MS.gene028062:CDS 35.0%
GTTAGAACTGCAATTGATAC+TGG + chr5.3:16724483-16724502 MS.gene028062:CDS 35.0%
TCAGTCATGATAACAGTAAG+TGG + chr5.3:16725535-16725554 MS.gene028062:CDS 35.0%
TGAACAAGTGAAATCCTTTC+AGG - chr5.3:16725495-16725514 None:intergenic 35.0%
TGATAATCATTGCTGCATTC+AGG - chr5.3:16723887-16723906 None:intergenic 35.0%
TTACAGAGATGGAGAAAGTA+TGG + chr5.3:16724040-16724059 MS.gene028062:intron 35.0%
! AAAACTACAGGGAGTGTATT+AGG - chr5.3:16725714-16725733 None:intergenic 35.0%
! ATTTATGTTAGGTTGCTAGC+TGG + chr5.3:16724652-16724671 MS.gene028062:intron 35.0%
! GAAGAATTCGGTGAATTTCT+TGG - chr5.3:16725110-16725129 None:intergenic 35.0%
! TCAAGACTGTCATCACATTT+TGG - chr5.3:16725357-16725376 None:intergenic 35.0%
! TGTGGTTTTTCATTCTACCT+TGG + chr5.3:16723749-16723768 MS.gene028062:CDS 35.0%
! TTTATGTTAGGTTGCTAGCT+GGG + chr5.3:16724653-16724672 MS.gene028062:intron 35.0%
AAGAAGTTGTTGCCAGATCT+TGG + chr5.3:16725579-16725598 MS.gene028062:CDS 40.0%
AAGAGATAACCATGAAGCTC+AGG + chr5.3:16724722-16724741 MS.gene028062:CDS 40.0%
AATACCTTTGGATCTGTGCA+TGG - chr5.3:16723920-16723939 None:intergenic 40.0%
ACAGGAATGTGCCATATGAT+TGG + chr5.3:16724313-16724332 MS.gene028062:intron 40.0%
ACGAACTAACACATAGACAC+CGG - chr5.3:16724588-16724607 None:intergenic 40.0%
ACGTATTCACCAACACCATT+AGG - chr5.3:16724420-16724439 None:intergenic 40.0%
AGAATCCATCAGCATGAAGT+AGG - chr5.3:16725923-16725942 None:intergenic 40.0%
AGAGCAATGAGCCATATCAA+AGG - chr5.3:16724826-16724845 None:intergenic 40.0%
AGATGACACCAAGAATTGCT+GGG - chr5.3:16724773-16724792 None:intergenic 40.0%
ATCAGCATGAAGTAGGTCAT+AGG - chr5.3:16725916-16725935 None:intergenic 40.0%
ATTGAGTGCATATTCACCTG+TGG - chr5.3:16725959-16725978 None:intergenic 40.0%
CAAATTCTGTCCATGGGATA+AGG - chr5.3:16724854-16724873 None:intergenic 40.0%
CAGATTTAAAGACTCCACCA+AGG - chr5.3:16723769-16723788 None:intergenic 40.0%
CCACAAAGGATCATTGATCA+AGG - chr5.3:16725664-16725683 None:intergenic 40.0%
CCCTTCAAACTCCTTTGATA+TGG + chr5.3:16724812-16724831 MS.gene028062:CDS 40.0%
CCGCAAAATAACTTGACTCT+CGG - chr5.3:16726499-16726518 None:intergenic 40.0%
CCTTGATCAATGATCCTTTG+TGG + chr5.3:16725661-16725680 MS.gene028062:CDS 40.0%
CTGCATTCAGGGAAACTAAA+GGG - chr5.3:16723875-16723894 None:intergenic 40.0%
CTTATCCCATGGACAGAATT+TGG + chr5.3:16724852-16724871 MS.gene028062:CDS 40.0%
CTTGGTGGAGTCTTTAAATC+TGG + chr5.3:16723767-16723786 MS.gene028062:CDS 40.0%
CTTTCCTCTCAAAAATCGGA+GGG - chr5.3:16724099-16724118 None:intergenic 40.0%
GCCATATCAAAGGAGTTTGA+AGG - chr5.3:16724816-16724835 None:intergenic 40.0%
GTAGAGATGAATGTTGGTAC+AGG + chr5.3:16724173-16724192 MS.gene028062:CDS 40.0%
GTCAGAGATAGGTTCTTGTT+CGG - chr5.3:16723704-16723723 None:intergenic 40.0%
GTTACAAACCTCCAATCAGA+TGG + chr5.3:16724143-16724162 MS.gene028062:CDS 40.0%
TAATGGCAAAAACACTTGCG+AGG + chr5.3:16726141-16726160 MS.gene028062:intron 40.0%
TATGACCTACTTCATGCTGA+TGG + chr5.3:16725915-16725934 MS.gene028062:CDS 40.0%
TCAAACATACCAGTCATGGA+AGG - chr5.3:16725757-16725776 None:intergenic 40.0%
TCAAAGGAGTTTGAAGGGAA+AGG - chr5.3:16724810-16724829 None:intergenic 40.0%
TCTTTCCTCTCAAAAATCGG+AGG - chr5.3:16724100-16724119 None:intergenic 40.0%
TGAAGTAGGTCATAGGTTCT+AGG - chr5.3:16725909-16725928 None:intergenic 40.0%
TGAGCTTCATGGTTATCTCT+TGG - chr5.3:16724723-16724742 None:intergenic 40.0%
TGATCCCAGGAATCAAAGAT+GGG + chr5.3:16724457-16724476 MS.gene028062:CDS 40.0%
TGTTAGTTCGTTGTTGCGTA+TGG + chr5.3:16724597-16724616 MS.gene028062:intron 40.0%
TTAATGCAAGATCTGATCCC+AGG + chr5.3:16724444-16724463 MS.gene028062:CDS 40.0%
! ACTGCAATTGATACTGGTTG+TGG + chr5.3:16724489-16724508 MS.gene028062:CDS 40.0%
! AGACTGTCATCACATTTTGG+AGG - chr5.3:16725354-16725373 None:intergenic 40.0%
! AGGTTGTTTGAGAGAGCTTT+TGG + chr5.3:16726161-16726180 MS.gene028062:intron 40.0%
! ATCATAGCAAGCGTTGTCTT+TGG - chr5.3:16725310-16725329 None:intergenic 40.0%
! ATCTTGTTTGTGCCAAGATC+TGG - chr5.3:16725594-16725613 None:intergenic 40.0%
! CTGCAATTGATACTGGTTGT+GGG + chr5.3:16724490-16724509 MS.gene028062:CDS 40.0%
! GGTTTTTCATTCTACCTTGG+TGG + chr5.3:16723752-16723771 MS.gene028062:CDS 40.0%
! TGCAATTGATACTGGTTGTG+GGG + chr5.3:16724491-16724510 MS.gene028062:CDS 40.0%
! TTATGTTAGGTTGCTAGCTG+GGG + chr5.3:16724654-16724673 MS.gene028062:intron 40.0%
!! CAGCATTGGTTGATAAAGGA+AGG + chr5.3:16724357-16724376 MS.gene028062:CDS 40.0%
!! CCGAGAGTCAAGTTATTTTG+CGG + chr5.3:16726496-16726515 MS.gene028062:CDS 40.0%
!! CTTAATTTGCAAAGAGCCTG+AGG - chr5.3:16723852-16723871 None:intergenic 40.0%
!! GGAGGTACTATGTTTCCTAA+TGG + chr5.3:16724402-16724421 MS.gene028062:CDS 40.0%
!! GTCATCACATTTTGGAGGAT+AGG - chr5.3:16725349-16725368 None:intergenic 40.0%
!! TTCAACCTTTGAGGCCATTT+CGG - chr5.3:16725465-16725484 None:intergenic 40.0%
!!! CGCTTGTTTTGTTGTTCCAA+TGG + chr5.3:16725407-16725426 MS.gene028062:CDS 40.0%
AAAGAGCCTGAGGATTGCTT+AGG - chr5.3:16723842-16723861 None:intergenic 45.0%
ACAACAAAACAAGCGCGAAG+CGG - chr5.3:16725402-16725421 None:intergenic 45.0%
ACATACCGAAATGGCCTCAA+AGG + chr5.3:16725457-16725476 MS.gene028062:CDS 45.0%
ACTGACCCATCTTTGATTCC+TGG - chr5.3:16724465-16724484 None:intergenic 45.0%
ATTCACCGAATTCTTCGTCC+CGG + chr5.3:16725114-16725133 MS.gene028062:CDS 45.0%
ATTGGAGGTTTGTAACCAGG+TGG - chr5.3:16724139-16724158 None:intergenic 45.0%
CAAACTGAACCTGAGCTTCA+TGG - chr5.3:16724734-16724753 None:intergenic 45.0%
CAAGAACCTATCTCTGACTG+CGG + chr5.3:16723706-16723725 MS.gene028062:CDS 45.0%
CAGTCAACTATGAGCGTAGA+TGG + chr5.3:16725163-16725182 MS.gene028062:CDS 45.0%
CATCTACGCTCATAGTTGAC+TGG - chr5.3:16725165-16725184 None:intergenic 45.0%
CTACTCTTTGGCCATCTGAT+TGG - chr5.3:16724157-16724176 None:intergenic 45.0%
CTGACCCATCTTTGATTCCT+GGG - chr5.3:16724464-16724483 None:intergenic 45.0%
CTGATCCCAGGAATCAAAGA+TGG + chr5.3:16724456-16724475 MS.gene028062:CDS 45.0%
CTGATTGGAGGTTTGTAACC+AGG - chr5.3:16724142-16724161 None:intergenic 45.0%
GACAAAACCCAAAATCCTCC+GGG - chr5.3:16725135-16725154 None:intergenic 45.0%
GAGATGACACCAAGAATTGC+TGG - chr5.3:16724774-16724793 None:intergenic 45.0%
GATAAGTCTAAGGGGCTGTT+TGG + chr5.3:16726097-16726116 MS.gene028062:intron 45.0%
GATTCACCTAAGCAATCCTC+AGG + chr5.3:16723833-16723852 MS.gene028062:CDS 45.0%
GCTGCATTCAGGGAAACTAA+AGG - chr5.3:16723876-16723895 None:intergenic 45.0%
GGACAAAACCCAAAATCCTC+CGG - chr5.3:16725136-16725155 None:intergenic 45.0%
GGTGCAACATTCAACCTTTG+AGG - chr5.3:16725474-16725493 None:intergenic 45.0%
GGTTGAATGTTGCACCTGAA+AGG + chr5.3:16725478-16725497 MS.gene028062:CDS 45.0%
GTCTTGAACCAGATTCAGCA+TGG + chr5.3:16725370-16725389 MS.gene028062:CDS 45.0%
TACTCCATGCACAGATCCAA+AGG + chr5.3:16723913-16723932 MS.gene028062:CDS 45.0%
TCTCAAAAATCGGAGGGCAA+TGG - chr5.3:16724093-16724112 None:intergenic 45.0%
TGGTATGCCAGAAAAAGCTG+TGG + chr5.3:16726603-16726622 MS.gene028062:CDS 45.0%
TTATCGGTACCTTCCATGAC+TGG + chr5.3:16725745-16725764 MS.gene028062:CDS 45.0%
TTGTGAGATCGCTCTAACAG+TGG + chr5.3:16726393-16726412 MS.gene028062:intron 45.0%
TTTGGAGGATAGGTGTCTCT+AGG - chr5.3:16725339-16725358 None:intergenic 45.0%
! CATCTTGATCCTGAGTTGGA+TGG - chr5.3:16726632-16726651 None:intergenic 45.0%
! CTATTGCGAAGGGTATGAGA+TGG + chr5.3:16726531-16726550 MS.gene028062:CDS 45.0%
!! AGTCATGGAAGGTACCGATA+AGG - chr5.3:16725746-16725765 None:intergenic 45.0%
!! ATTGCCCTCCGATTTTTGAG+AGG + chr5.3:16724092-16724111 MS.gene028062:CDS 45.0%
!! GTTTTCGATCGAGGCCTTAT+CGG + chr5.3:16725729-16725748 MS.gene028062:CDS 45.0%
!!! GGAGGATTTTGGGTTTTGTC+CGG + chr5.3:16725135-16725154 MS.gene028062:CDS 45.0%
AACTATGAGCGTAGATGGCG+CGG + chr5.3:16725168-16725187 MS.gene028062:CDS 50.0%
ACCCTTCGCAATAGTTGCGA+TGG - chr5.3:16726525-16726544 None:intergenic 50.0%
AGGCCTCGATCGAAAACTAC+AGG - chr5.3:16725726-16725745 None:intergenic 50.0%
ATAGGTTCTTGTTCGGCTGG+TGG - chr5.3:16723697-16723716 None:intergenic 50.0%
ATCCTCCGGGACGAAGAATT+CGG - chr5.3:16725122-16725141 None:intergenic 50.0%
CTACGCTCATAGTTGACTGG+TGG - chr5.3:16725162-16725181 None:intergenic 50.0%
CTCTTTGGCCATCTGATTGG+AGG - chr5.3:16724154-16724173 None:intergenic 50.0%
GAGATAGGTTCTTGTTCGGC+TGG - chr5.3:16723700-16723719 None:intergenic 50.0%
GCCATCGCAACTATTGCGAA+GGG + chr5.3:16726521-16726540 MS.gene028062:CDS 50.0%
GCCATTTCGGTATGTACGTG+AGG - chr5.3:16725452-16725471 None:intergenic 50.0%
GCCTCACGTACATACCGAAA+TGG + chr5.3:16725448-16725467 MS.gene028062:CDS 50.0%
GGAAACATAGTACCTCCACC+AGG - chr5.3:16724399-16724418 None:intergenic 50.0%
GGAGTGTACCATGCTGAATC+TGG - chr5.3:16725381-16725400 None:intergenic 50.0%
GGCCTCGATCGAAAACTACA+GGG - chr5.3:16725725-16725744 None:intergenic 50.0%
GTCTAAGGGGCTGTTTGGAT+TGG + chr5.3:16726102-16726121 MS.gene028062:intron 50.0%
TCTTCACTGCTGAAAGCCAC+AGG + chr5.3:16725940-16725959 MS.gene028062:CDS 50.0%
TGCCATCGCAACTATTGCGA+AGG + chr5.3:16726520-16726539 MS.gene028062:CDS 50.0%
! CTCATAGTTGACTGGTGGAC+CGG - chr5.3:16725157-16725176 None:intergenic 50.0%
! CTCCCTGTAGTTTTCGATCG+AGG + chr5.3:16725720-16725739 MS.gene028062:CDS 50.0%
! GCTCTAGATGCCTTATCCCA+TGG + chr5.3:16724841-16724860 MS.gene028062:CDS 50.0%
! TCTTCGTCCCGGAGGATTTT+GGG + chr5.3:16725125-16725144 MS.gene028062:CDS 50.0%
! TTCTTCGTCCCGGAGGATTT+TGG + chr5.3:16725124-16725143 MS.gene028062:CDS 50.0%
!! ATGGCTGCCACAGCTTTTTC+TGG - chr5.3:16726613-16726632 None:intergenic 50.0%
ATGGCGCGGATGGAACACAA+CGG + chr5.3:16725182-16725201 MS.gene028062:CDS 55.0%
CGCGGTATCCCAGCAATTCT+TGG + chr5.3:16724762-16724781 MS.gene028062:CDS 55.0%
GGAGGTTTGTAACCAGGTGG+AGG - chr5.3:16724136-16724155 None:intergenic 55.0%
GGTTTGTAACCAGGTGGAGG+AGG - chr5.3:16724133-16724152 None:intergenic 55.0%
TGATCGCCGCAGTCAGAGAT+AGG - chr5.3:16723715-16723734 None:intergenic 55.0%
TGCTTAGTTCCTCCTCCACC+TGG + chr5.3:16724121-16724140 MS.gene028062:CDS 55.0%
! ATGGACCGGATTTTGCGACC+AGG + chr5.3:16726458-16726477 MS.gene028062:CDS 55.0%
! GTTCAGTTTGCTCTCGAGCG+CGG + chr5.3:16724744-16724763 MS.gene028062:CDS 55.0%
! TGTTAGGTTGCTAGCTGGGG+CGG + chr5.3:16724657-16724676 MS.gene028062:intron 55.0%
ATGAGCGTAGATGGCGCGGA+TGG + chr5.3:16725172-16725191 MS.gene028062:CDS 60.0%
CACCGAATTCTTCGTCCCGG+AGG + chr5.3:16725117-16725136 MS.gene028062:CDS 60.0%
CTGTGGCAGCCATCCAACTC+AGG + chr5.3:16726620-16726639 MS.gene028062:CDS 60.0%
! ACCGGATTTTGCGACCAGGC+GGG + chr5.3:16726462-16726481 MS.gene028062:CDS 60.0%
! GACCGGATTTTGCGACCAGG+CGG + chr5.3:16726461-16726480 MS.gene028062:CDS 60.0%
! TAGCTGGGGCGGTGACTTGT+TGG + chr5.3:16724668-16724687 MS.gene028062:CDS 60.0%
!! GGCGGTGACTTGTTGGATCG+AGG + chr5.3:16724675-16724694 MS.gene028062:CDS 60.0%
CGGATTATAGCGTGCCCGCC+TGG - chr5.3:16726479-16726498 None:intergenic 65.0%
GCCCGCCTGGTCGCAAAATC+CGG - chr5.3:16726466-16726485 None:intergenic 65.0%
GCGCGGATGGAACACAACGG+TGG + chr5.3:16725185-16725204 MS.gene028062:CDS 65.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 16723668 16726652 16723668 ID=MS.gene028062
chr5.3 mRNA 16723668 16726652 16723668 ID=MS.gene028062.t1;Parent=MS.gene028062
chr5.3 exon 16723668 16723934 16723668 ID=MS.gene028062.t1.exon1;Parent=MS.gene028062.t1
chr5.3 CDS 16723668 16723934 16723668 ID=cds.MS.gene028062.t1;Parent=MS.gene028062.t1
chr5.3 exon 16724046 16724194 16724046 ID=MS.gene028062.t1.exon2;Parent=MS.gene028062.t1
chr5.3 CDS 16724046 16724194 16724046 ID=cds.MS.gene028062.t1;Parent=MS.gene028062.t1
chr5.3 exon 16724317 16724512 16724317 ID=MS.gene028062.t1.exon3;Parent=MS.gene028062.t1
chr5.3 CDS 16724317 16724512 16724317 ID=cds.MS.gene028062.t1;Parent=MS.gene028062.t1
chr5.3 exon 16724663 16724873 16724663 ID=MS.gene028062.t1.exon4;Parent=MS.gene028062.t1
chr5.3 CDS 16724663 16724873 16724663 ID=cds.MS.gene028062.t1;Parent=MS.gene028062.t1
chr5.3 exon 16725094 16725766 16725094 ID=MS.gene028062.t1.exon5;Parent=MS.gene028062.t1
chr5.3 CDS 16725094 16725766 16725094 ID=cds.MS.gene028062.t1;Parent=MS.gene028062.t1
chr5.3 exon 16725884 16725961 16725884 ID=MS.gene028062.t1.exon6;Parent=MS.gene028062.t1
chr5.3 CDS 16725884 16725961 16725884 ID=cds.MS.gene028062.t1;Parent=MS.gene028062.t1
chr5.3 exon 16726430 16726652 16726430 ID=MS.gene028062.t1.exon7;Parent=MS.gene028062.t1
chr5.3 CDS 16726430 16726652 16726430 ID=cds.MS.gene028062.t1;Parent=MS.gene028062.t1
Gene Sequence

>MS.gene028062

ATGAAGCATAAGGATGGAAAACTGATCCACCAGCCGAACAAGAACCTATCTCTGACTGCGGCGATCACTATCATTGTTCTGTGTGGTTTTTCATTCTACCTTGGTGGAGTCTTTAAATCTGGAAACAATGGTGTTGATGTCATCAACACCATTCAGAAATCACTTGATTCACCTAAGCAATCCTCAGGCTCTTTGCAAATTAAGCCCTTTAGTTTCCCTGAATGCAGCAATGATTATCAAGACTATACTCCATGCACAGATCCAAAGGTATTAATTAATACTAATAGTTTTCTACAAAAATTTGGATGTTTGGATTCTTTTATAGAAGAAAATTTCTTTATTTTGTTGATTGAGTTTTGAGAATGGATGATTTTACAGAGATGGAGAAAGTATGGTACCTATAGACTTACTTTGTTGGAACGCCATTGCCCTCCGATTTTTGAGAGGAAAGAATGCTTAGTTCCTCCTCCACCTGGTTACAAACCTCCAATCAGATGGCCAAAGAGTAGAGATGAATGTTGGTACAGGTATGTAATAGATGATTTATAAGTTGAAATAATTATAGTTTGAATTGATTTTATGTTGCTTGCTACATCGATATATATATATTGTTCAAGTTTTTAATTTGTGTTACATGAATAAATGACAGGAATGTGCCATATGATTGGATTAACAAACAGAAGTCTAATCAGCATTGGTTGATAAAGGAAGGAGAGAAATTTCAATTTCCTGGTGGAGGTACTATGTTTCCTAATGGTGTTGGTGAATACGTTGATTTAATGCAAGATCTGATCCCAGGAATCAAAGATGGGTCAGTTAGAACTGCAATTGATACTGGTTGTGGGGTGAGTTTTATCCTTCTTTTTACTTTTACTTCTTTTTACAATTGGCCGCTTTCTTTGTATTTTTACTTATTACCGGTGTCTATGTGTTAGTTCGTTGTTGCGTATGGTGTATGTGTTAATTGATATCCACATAACTCCGATTTATGTTAGGTTGCTAGCTGGGGCGGTGACTTGTTGGATCGAGGCGTTCTAACAATTTCTCTCGCGCCAAGAGATAACCATGAAGCTCAGGTTCAGTTTGCTCTCGAGCGCGGTATCCCAGCAATTCTTGGTGTCATCTCTACACAAAGACTTCCTTTCCCTTCAAACTCCTTTGATATGGCTCATTGCTCTAGATGCCTTATCCCATGGACAGAATTTGGTAATTCTTCAATGCATTTTAAAATTTTCAGTTCTTAAAACAGTTTTTTTTTCCTTTCTGTTTATGCTATGTTTTCATGAACTTTACCAGCATAATGAAACAGTTTCATTCATCTAAGTTATACATTATTTTCATGAACTGTCTTGTTTAGAGCTGATATTAGTTCAGTTGAAATTTTCATCCATTAACTGATGGGAATATTCATATCTTATGCTTTTAGGTGGAATTTATCTCCAAGAAATTCACCGAATTCTTCGTCCCGGAGGATTTTGGGTTTTGTCCGGTCCACCAGTCAACTATGAGCGTAGATGGCGCGGATGGAACACAACGGTGGAAGAACAAAGATCAGATTATGAAAAGCTACAAGATTTGCTCACATCAATGTGCTTCAAATTATACAACAAAAAGGATGACATATATGTATGGCAAAAGGCCAAAGACAACGCTTGCTATGATAAGCTTCCTAGAGACACCTATCCTCCAAAATGTGATGACAGTCTTGAACCAGATTCAGCATGGTACACTCCGCTTCGCGCTTGTTTTGTTGTTCCAATGGAAAAGTACAAGAAATCAGGCCTCACGTACATACCGAAATGGCCTCAAAGGTTGAATGTTGCACCTGAAAGGATTTCACTTGTTCAAGGTTCTAGTTCTAGTACTTTCAGTCATGATAACAGTAAGTGGAAAAAGCGCATTCAGCACTACAAGAAGTTGTTGCCAGATCTTGGCACAAACAAGATAAGAAATGTGATGGATATGAATACAGCTTATGGAGGTTTCGCTGCTTCCTTGATCAATGATCCTTTGTGGGTTATGAATGTTGTTTCATCTTATGGTCCTAATACACTCCCTGTAGTTTTCGATCGAGGCCTTATCGGTACCTTCCATGACTGGTATGTTTGAAACTCTACATATGAACCATAAGGTTATGTGTTTATGAATGTTTTTGTGTTATATCATATGGTTTTCTTTGGTTATGATTTGAATTTTATGATGACTTGTTCTATTAGGTGTGAAGCTTTTTCAACATATCCTAGAACCTATGACCTACTTCATGCTGATGGATTCTTCACTGCTGAAAGCCACAGGTGAATATGCACTCAATCAATACTTCAATTTTGACAGCTTAATTAATCACTTCTTGAATACGTATTAATAATATAAACACAAGGAGCATACTGAAAACAGCATACTGAACACTTTCACAGTTGCTTTTGTTATAAGATAAGTCTAAGGGGCTGTTTGGATTGGCTTGTTTGAGCTTATCTAATGGCAAAAACACTTGCGAGGTTGTTTGAGAGAGCTTTTGGAAACAACTTATAACATGTCAATAAGTTGTTTTTAGTTTATTTTTATAAGCTCTCTATTATAGCTTATGAAAATAAGTTGACTTTATTTTATCTTTTATAACAGAAAGAGCTTATACATAAGCACTTATATGACAAAATCTTATGTTATAAGTGCTTAATTAGGTTGTTTATCCAAACTTGGCCTAAATAAGATTTTCAAAACGCACTCATTGTGAGATCGCTCTAACAGTGGCTGTTACTTTGCAGATGTGAAATGAAGTATGTAATGTTGGAAATGGACCGGATTTTGCGACCAGGCGGGCACGCTATAATCCGAGAGTCAAGTTATTTTGCGGATGCCATCGCAACTATTGCGAAGGGTATGAGATGGATATGTCACAAAGAAAACACTGAGTTTGGTGTTGAAAAAGAAAAGATATTGGTATGCCAGAAAAAGCTGTGGCAGCCATCCAACTCAGGATCAAGATGA
Protein sequence

>MS.gene028062.t1

MKHKDGKLIHQPNKNLSLTAAITIIVLCGFSFYLGGVFKSGNNGVDVINTIQKSLDSPKQSSGSLQIKPFSFPECSNDYQDYTPCTDPKRWRKYGTYRLTLLERHCPPIFERKECLVPPPPGYKPPIRWPKSRDECWYRNVPYDWINKQKSNQHWLIKEGEKFQFPGGGTMFPNGVGEYVDLMQDLIPGIKDGSVRTAIDTGCGVASWGGDLLDRGVLTISLAPRDNHEAQVQFALERGIPAILGVISTQRLPFPSNSFDMAHCSRCLIPWTEFGGIYLQEIHRILRPGGFWVLSGPPVNYERRWRGWNTTVEEQRSDYEKLQDLLTSMCFKLYNKKDDIYVWQKAKDNACYDKLPRDTYPPKCDDSLEPDSAWYTPLRACFVVPMEKYKKSGLTYIPKWPQRLNVAPERISLVQGSSSSTFSHDNSKWKKRIQHYKKLLPDLGTNKIRNVMDMNTAYGGFAASLINDPLWVMNVVSSYGPNTLPVVFDRGLIGTFHDWCEAFSTYPRTYDLLHADGFFTAESHRCEMKYVMLEMDRILRPGGHAIIRESSYFADAIATIAKGMRWICHKENTEFGVEKEKILVCQKKLWQPSNSGSR