AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene073679

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene073679.t1 MTR_1g051055 97.368 608 14 1 1 608 1 606 0.0 1237
MS.gene073679.t1 MTR_1g442840 41.013 612 343 8 8 605 8 615 5.19e-157 464
MS.gene073679.t1 MTR_1g069785 46.310 542 262 10 81 605 56 585 2.20e-156 462
MS.gene073679.t1 MTR_1g442840 45.908 501 259 6 114 605 5 502 6.13e-152 447
MS.gene073679.t1 MTR_1g075900 44.952 525 277 6 90 605 93 614 5.55e-151 449
MS.gene073679.t1 MTR_5g080720 44.061 522 274 7 89 602 126 637 3.10e-144 432
MS.gene073679.t1 MTR_8g432620 40.566 530 290 9 88 607 88 602 1.37e-136 411
MS.gene073679.t1 MTR_3g068990 43.378 521 277 7 90 602 155 665 1.37e-136 414
MS.gene073679.t1 MTR_7g076150 40.566 530 290 9 88 607 75 589 2.49e-135 407
MS.gene073679.t1 MTR_5g080720 46.563 451 226 6 89 531 126 569 1.50e-134 405
MS.gene073679.t1 MTR_1g029730 41.985 524 287 9 90 604 143 658 3.84e-132 402
MS.gene073679.t1 MTR_8g100075 38.390 534 298 8 91 606 101 621 4.57e-130 395
MS.gene073679.t1 MTR_3g088790 42.778 540 250 17 96 605 232 742 2.62e-129 397
MS.gene073679.t1 MTR_1g101370 41.978 536 279 10 91 606 95 618 3.90e-129 393
MS.gene073679.t1 MTR_7g076170 38.419 544 296 10 86 606 99 626 1.62e-126 387
MS.gene073679.t1 MTR_0019s0120 40.260 539 280 12 91 606 108 627 1.76e-126 386
MS.gene073679.t1 MTR_8g071280 39.698 529 292 10 91 605 73 588 8.21e-125 381
MS.gene073679.t1 MTR_5g040360 39.098 532 288 11 91 605 87 599 1.99e-124 380
MS.gene073679.t1 MTR_3g040370 38.941 529 290 9 91 605 88 597 6.68e-124 379
MS.gene073679.t1 MTR_8g432680 35.285 649 352 16 4 606 5 631 3.19e-123 378
MS.gene073679.t1 MTR_8g055840 38.983 531 291 10 91 605 87 600 3.52e-123 377
MS.gene073679.t1 MTR_5g083150 40.462 519 278 11 97 605 101 598 4.41e-122 374
MS.gene073679.t1 MTR_8g093880 38.898 581 315 17 47 605 244 806 2.75e-121 379
MS.gene073679.t1 MTR_4g098890 40.613 522 266 11 91 591 89 587 1.54e-120 391
MS.gene073679.t1 MTR_8g076650 41.412 524 272 14 97 605 387 890 7.56e-120 377
MS.gene073679.t1 MTR_5g026930 38.308 603 315 15 23 605 25 590 9.74e-120 368
MS.gene073679.t1 MTR_5g021100 39.024 574 304 15 49 605 289 833 3.82e-119 374
MS.gene073679.t1 MTR_7g006060 39.810 525 278 12 80 581 35 544 2.75e-118 363
MS.gene073679.t1 MTR_3g096480 37.755 588 310 17 47 605 216 776 2.55e-117 367
MS.gene073679.t1 MTR_3g071530 38.521 514 291 8 97 605 153 646 9.67e-116 359
MS.gene073679.t1 MTR_7g076150 41.319 455 246 8 163 607 3 446 6.13e-115 350
MS.gene073679.t1 MTR_0019s0120 39.955 443 227 11 91 510 108 534 4.71e-98 310
MS.gene073679.t1 MTR_3g074780 34.615 546 318 11 88 605 154 688 6.16e-96 308
MS.gene073679.t1 MTR_4g083030 33.279 616 343 20 23 604 22 603 1.45e-95 305
MS.gene073679.t1 MTR_5g064080 34.982 546 306 12 88 605 146 670 6.69e-94 303
MS.gene073679.t1 MTR_5g026930 37.795 508 263 13 23 510 25 499 1.49e-93 296
MS.gene073679.t1 MTR_1g022255 32.625 518 311 12 98 605 210 699 5.38e-85 280
MS.gene073679.t1 MTR_1g022255 29.427 384 239 10 98 471 210 571 3.86e-45 169
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene073679.t1 AT3G10200 68.614 599 169 6 13 608 9 591 0.0 894
MS.gene073679.t1 AT5G04060 68.144 609 181 5 2 608 3 600 0.0 890
MS.gene073679.t1 AT3G10200 68.382 544 154 5 14 555 10 537 0.0 813
MS.gene073679.t1 AT1G04430 44.963 536 267 7 90 605 86 613 6.29e-153 454
MS.gene073679.t1 AT1G04430 44.963 536 267 7 90 605 86 613 6.29e-153 454
MS.gene073679.t1 AT1G04430 44.963 536 267 7 90 605 86 613 6.29e-153 454
MS.gene073679.t1 AT3G23300 44.656 524 279 7 90 605 81 601 1.98e-149 444
MS.gene073679.t1 AT3G23300 44.656 524 279 7 90 605 81 601 1.98e-149 444
MS.gene073679.t1 AT5G14430 43.697 595 310 9 24 607 26 606 4.04e-149 444
MS.gene073679.t1 AT5G14430 43.361 595 312 9 24 607 26 606 7.89e-148 441
MS.gene073679.t1 AT4G14360 44.084 524 282 6 90 605 78 598 1.03e-147 440
MS.gene073679.t1 AT4G14360 44.084 524 282 6 90 605 78 598 1.03e-147 440
MS.gene073679.t1 AT5G06050 42.667 525 281 8 89 604 150 663 1.60e-139 422
MS.gene073679.t1 AT1G77260 42.589 533 283 9 84 604 134 655 2.08e-138 418
MS.gene073679.t1 AT2G39750 39.613 568 318 7 44 604 142 691 2.48e-138 419
MS.gene073679.t1 AT4G19120 37.152 611 332 13 14 605 13 590 2.77e-129 392
MS.gene073679.t1 AT4G19120 37.152 611 332 13 14 605 13 590 2.77e-129 392
MS.gene073679.t1 AT4G00740 39.700 534 289 10 88 607 81 595 8.67e-129 391
MS.gene073679.t1 AT4G00740 39.700 534 289 10 88 607 81 595 8.67e-129 391
MS.gene073679.t1 AT4G10440 38.235 544 286 10 91 606 96 617 1.90e-127 389
MS.gene073679.t1 AT1G33170 38.309 556 305 11 75 606 94 635 2.98e-127 389
MS.gene073679.t1 AT1G26850 39.179 536 289 10 91 605 89 608 6.59e-127 387
MS.gene073679.t1 AT1G26850 39.179 536 289 10 91 605 89 608 6.59e-127 387
MS.gene073679.t1 AT2G45750 38.879 553 301 11 80 607 77 617 2.00e-126 386
MS.gene073679.t1 AT1G29470 40.569 562 300 16 61 605 213 757 3.71e-126 390
MS.gene073679.t1 AT1G29470 40.569 562 300 16 61 605 213 757 3.71e-126 390
MS.gene073679.t1 AT2G34300 41.856 528 268 14 97 605 250 757 1.23e-124 386
MS.gene073679.t1 AT2G34300 41.856 528 268 14 97 605 250 757 1.23e-124 386
MS.gene073679.t1 AT2G34300 41.856 528 268 14 97 605 250 757 1.23e-124 386
MS.gene073679.t1 AT1G31850 38.971 544 299 12 80 605 68 596 2.52e-124 380
MS.gene073679.t1 AT1G31850 38.971 544 299 12 80 605 68 596 2.52e-124 380
MS.gene073679.t1 AT1G31850 38.971 544 299 12 80 605 68 596 2.52e-124 380
MS.gene073679.t1 AT1G31850 38.971 544 299 12 80 605 68 596 2.52e-124 380
MS.gene073679.t1 AT2G43200 40.909 528 279 12 90 599 93 605 7.51e-124 379
MS.gene073679.t1 AT2G43200 40.909 528 279 12 90 599 93 605 7.51e-124 379
MS.gene073679.t1 AT4G00750 37.585 588 322 12 45 606 55 623 8.57e-123 377
MS.gene073679.t1 AT2G40280 39.922 516 279 14 97 605 90 581 6.62e-122 373
MS.gene073679.t1 AT3G56080 37.987 616 314 16 7 580 2 591 1.17e-121 373
MS.gene073679.t1 AT3G51070 39.086 591 311 17 46 605 317 889 5.02e-121 380
MS.gene073679.t1 AT4G18030 38.649 533 292 10 91 605 88 603 1.77e-120 370
MS.gene073679.t1 AT5G64030 40.458 524 282 13 97 605 308 816 2.03e-120 376
MS.gene073679.t1 AT3G56080 38.698 553 282 13 18 535 19 549 1.01e-111 345
MS.gene073679.t1 AT3G51070 39.575 518 271 14 46 532 317 823 3.61e-106 339
MS.gene073679.t1 AT4G19120 36.240 516 281 11 14 510 13 499 3.31e-101 317
MS.gene073679.t1 AT1G13860 34.375 544 298 15 90 602 83 598 4.36e-95 304
MS.gene073679.t1 AT1G13860 34.375 544 298 15 90 602 83 598 4.36e-95 304
MS.gene073679.t1 AT1G13860 34.375 544 298 15 90 602 83 598 4.36e-95 304
MS.gene073679.t1 AT1G13860 34.375 544 298 15 90 602 83 598 4.36e-95 304
MS.gene073679.t1 AT1G78240 33.640 544 323 11 88 604 146 678 2.09e-94 304
MS.gene073679.t1 AT1G78240 33.640 544 323 11 88 604 146 678 2.09e-94 304
MS.gene073679.t1 AT2G03480 32.062 577 307 15 67 604 62 592 1.05e-90 292
MS.gene073679.t1 AT1G19430 34.489 519 301 14 98 605 232 722 1.90e-88 290
MS.gene073679.t1 AT1G19430 34.489 519 301 14 98 605 232 722 1.90e-88 290
MS.gene073679.t1 AT1G26850 36.449 428 239 8 91 498 89 503 5.10e-88 283
MS.gene073679.t1 AT2G03480 32.062 577 318 16 67 604 62 603 1.65e-87 284
MS.gene073679.t1 AT1G13860 36.009 436 240 12 194 602 19 442 1.49e-80 261
MS.gene073679.t1 AT2G03480 31.564 358 208 10 67 404 62 402 1.84e-49 178
MS.gene073679.t1 AT1G19430 31.688 385 232 11 98 472 232 595 4.63e-48 177

Find 129 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTACATCCCAAAGGAATTT+TGG 0.124701 1.1:+35816275 None:intergenic
CCTTTGGATGGACCCAATTT+TGG 0.176094 1.1:+35823052 None:intergenic
CGTATAGACTTTCATGAAAA+TGG 0.234122 1.1:-35820138 MS.gene073679:intron
ATGTCAAAGGAGGCCAAAAT+TGG 0.261908 1.1:-35823065 MS.gene073679:CDS
AACGGCAAATCCACCATAAA+AGG 0.279828 1.1:+35816985 None:intergenic
GATCATCTCTGGTGGTTTCC+TGG 0.288695 1.1:-35823030 MS.gene073679:CDS
TCTGCCTTTGATTCTAAATC+TGG 0.293833 1.1:-35823744 MS.gene073679:CDS
GTACGAGGATAACTTGAAAA+TGG 0.319695 1.1:+35816753 None:intergenic
AAACAACTGCCATTTCCTTC+AGG 0.326414 1.1:-35820192 MS.gene073679:CDS
CATTGAACAACTTTCCTGTT+TGG 0.339170 1.1:-35816961 MS.gene073679:CDS
TGCAGGGATTTATTATCATT+AGG 0.340512 1.1:-35816337 MS.gene073679:intron
GCAGGGATTTATTATCATTA+GGG 0.340665 1.1:-35816336 MS.gene073679:intron
GCTCTAATGCAAATTGAATC+TGG 0.351997 1.1:+35820250 None:intergenic
GTAAAGAAGCCATTATGATT+TGG 0.353865 1.1:+35823721 None:intergenic
ATTGAACAACTTTCCTGTTT+GGG 0.357492 1.1:-35816960 MS.gene073679:CDS
TGCGGGCATAGTTCAAGTTC+TGG 0.360065 1.1:-35820364 MS.gene073679:CDS
CTATCAGATGGCCTCTTAGT+AGG 0.363179 1.1:-35823131 MS.gene073679:CDS
CTCTTAGTAGGGATTATGTT+TGG 0.366295 1.1:-35823119 MS.gene073679:CDS
TTACATCAGATTCTATCTTC+TGG 0.370766 1.1:-35817084 MS.gene073679:CDS
AAGATCAGACTCGTCATTAT+TGG 0.376187 1.1:-35817060 MS.gene073679:CDS
ATTTATGACCGGGGTTTGAT+TGG 0.378010 1.1:-35816893 MS.gene073679:CDS
ATAGTTCAAGTTCTGGATCT+TGG 0.379508 1.1:-35820357 MS.gene073679:CDS
ATTTAGCCCCAAAATTCCTT+TGG 0.384533 1.1:-35816283 MS.gene073679:CDS
GGCATGAAGAACACCTTGTC+TGG 0.400833 1.1:-35816917 MS.gene073679:CDS
ATGATGAGTCTAAACCTTCA+TGG 0.401456 1.1:-35817881 MS.gene073679:CDS
TTTGCATTAGAGCGAGGAAT+AGG 0.404400 1.1:-35820240 MS.gene073679:CDS
TAAATCTGGCCAAATCATAA+TGG 0.406380 1.1:-35823730 MS.gene073679:CDS
TCCTGTCATTTGGGATAAGT+TGG 0.410218 1.1:-35818039 MS.gene073679:CDS
TTCTGCATTCTGCTGAAGGC+AGG 0.416327 1.1:+35817932 None:intergenic
TCAAATGACCCTGAAGGAAA+TGG 0.422002 1.1:+35820183 None:intergenic
TAGCCCTAGGTTTGTGTACT+GGG 0.422018 1.1:+35816698 None:intergenic
AGGGCTAGGTTGTATATTAG+AGG 0.425165 1.1:-35816682 MS.gene073679:CDS
TTTAGCCCCAAAATTCCTTT+GGG 0.425741 1.1:-35816282 MS.gene073679:CDS
GTAAAGTTCAGACTGCAATA+TGG 0.426050 1.1:-35817977 MS.gene073679:CDS
ACTGAAAGGCGCTCGTGACT+TGG 0.432443 1.1:+35817795 None:intergenic
TTCAGCGTATCTTCTTCCCT+TGG 0.432592 1.1:-35820316 MS.gene073679:CDS
ACTACATTCATTACCCAAAC+AGG 0.436158 1.1:+35816947 None:intergenic
CTGAAAGGCGCTCGTGACTT+GGG 0.436945 1.1:+35817796 None:intergenic
TTGTTTGGTTCCGCCGCCTA+AGG 0.437102 1.1:-35823166 MS.gene073679:CDS
TCTGAAAAGCTGAACAAGAT+AGG 0.447938 1.1:-35817768 MS.gene073679:intron
CTACTAAGAGGCCATCTGAT+AGG 0.448095 1.1:+35823132 None:intergenic
CTAGCCCTAGGTTTGTGTAC+TGG 0.452424 1.1:+35816697 None:intergenic
AACAACTGCCATTTCCTTCA+GGG 0.458335 1.1:-35820191 MS.gene073679:CDS
GATCGTCTTCTTCGCTCAAA+TGG 0.459443 1.1:-35818110 MS.gene073679:CDS
ACTTGTATACAGTTCTTAAG+TGG 0.464593 1.1:+35817852 None:intergenic
CATATATCTACCCCAGTGTC+AGG 0.465947 1.1:+35823318 None:intergenic
CTCTGGTGGTTTCCTGGTGG+TGG 0.466744 1.1:-35823024 MS.gene073679:CDS
AACCACCAGAGATGATCCTT+TGG 0.471865 1.1:+35823036 None:intergenic
CACTTACCGCAAAATTCCCT+TGG 0.471979 1.1:-35823346 MS.gene073679:CDS
TTTGTTCTGCATTCTGCTGA+AGG 0.474756 1.1:+35817928 None:intergenic
GATATACTCATTAAACTTCA+AGG 0.474981 1.1:+35823293 None:intergenic
CCTTGGTAATTCCTGACACT+GGG 0.480156 1.1:-35823329 MS.gene073679:CDS
GACTGGTGATTTACGTTCTG+CGG 0.489853 1.1:-35820382 MS.gene073679:CDS
AGAGGATATCATGCTGGAGA+TGG 0.496478 1.1:-35816664 MS.gene073679:CDS
GGATCGTCTTGCACGGCCAC+TGG 0.497371 1.1:-35816643 MS.gene073679:intron
AAGAAGATACGCTGAAAAGC+TGG 0.500896 1.1:+35820323 None:intergenic
AGAGAACAAAGAAAAGAAGA+TGG 0.501740 1.1:-35816240 MS.gene073679:CDS
TATCAGATGGCCTCTTAGTA+GGG 0.502303 1.1:-35823130 MS.gene073679:CDS
GGAAGGTGAGAGATGTAAAG+AGG 0.503623 1.1:+35823648 None:intergenic
TCCATCCAAAGGATCATCTC+TGG 0.505245 1.1:-35823041 MS.gene073679:CDS
AATGAAGAAGGAGAGGAAGA+AGG 0.507870 1.1:+35823627 None:intergenic
CCCTTGGTAATTCCTGACAC+TGG 0.510885 1.1:-35823330 MS.gene073679:CDS
CAAGTTCTGGATCTTGGCTG+TGG 0.512542 1.1:-35820351 MS.gene073679:CDS
TTAGTAGGGATTATGTTTGG+AGG 0.514597 1.1:-35823116 MS.gene073679:CDS
CCTTGTCTGGAATTTATGAC+CGG 0.514633 1.1:-35816904 MS.gene073679:CDS
AAGAAGGAGAGGAAGAAGGA+AGG 0.516792 1.1:+35823631 None:intergenic
AAGGTGTTCTTCATGCCTGC+AGG 0.523680 1.1:+35816923 None:intergenic
GGTGGTACTCATTTCAAGAA+TGG 0.526261 1.1:-35823006 MS.gene073679:CDS
CAAACATAATCCCTACTAAG+AGG 0.526307 1.1:+35823120 None:intergenic
ACTGGTGATTTACGTTCTGC+GGG 0.530564 1.1:-35820381 MS.gene073679:CDS
TGTCAAAGGAGGCCAAAATT+GGG 0.535636 1.1:-35823064 MS.gene073679:CDS
TGTTTCTAGAAAACAAGAGC+TGG 0.539783 1.1:-35823223 MS.gene073679:CDS
GTGTTGTTGTTGAATGAAGA+AGG 0.541421 1.1:+35823615 None:intergenic
GGTTTGTGTACTGGGAAAAG+AGG 0.547984 1.1:+35816706 None:intergenic
GCGGAACCAAACAAAACAAA+CGG 0.549143 1.1:+35823175 None:intergenic
CTTGTATACAGTTCTTAAGT+GGG 0.549205 1.1:+35817853 None:intergenic
TTGGCCAGATTTAGAATCAA+AGG 0.550068 1.1:+35823740 None:intergenic
TGAAAGGCGCTCGTGACTTG+GGG 0.551470 1.1:+35817797 None:intergenic
TGCATTCTGCTGAAGGCAGG+AGG 0.551507 1.1:+35817935 None:intergenic
AGACATAGTTCGAATATCCA+AGG 0.552080 1.1:+35820299 None:intergenic
TATATTAGAGGATATCATGC+TGG 0.556437 1.1:-35816670 MS.gene073679:CDS
AATATGATTACAAATGAGAC+TGG 0.557833 1.1:-35820399 MS.gene073679:CDS
CCAAAATTGGGTCCATCCAA+AGG 0.558669 1.1:-35823052 MS.gene073679:CDS
CTAATATACAACCTAGCCCT+AGG 0.565807 1.1:+35816685 None:intergenic
ACTTGACTACAGCAATGTGT+TGG 0.565940 1.1:-35818013 MS.gene073679:CDS
ACCAACTTATCCCAAATGAC+AGG 0.566105 1.1:+35818038 None:intergenic
AGAATCAAAGGCAGAACCAA+TGG 0.566374 1.1:+35823752 None:intergenic
TGGGTAATTACCGTTAGAAG+AGG 0.569025 1.1:+35823590 None:intergenic
AACAGAAATACGCAACGTGA+TGG 0.574714 1.1:-35817017 MS.gene073679:CDS
ATTATAAAATACCTATCAGA+TGG 0.575123 1.1:-35823143 MS.gene073679:CDS
GTAATGAATGTAGTTCCTGC+AGG 0.579842 1.1:-35816938 MS.gene073679:CDS
CTTGTCTGGAATTTATGACC+GGG 0.580796 1.1:-35816903 MS.gene073679:CDS
TCTGGATCTTGGCTGTGGTG+TGG 0.587155 1.1:-35820346 MS.gene073679:CDS
ACACATCTTGCTGATGTCAA+AGG 0.587482 1.1:-35823078 MS.gene073679:CDS
ACCAGAGATGATCCTTTGGA+TGG 0.587544 1.1:+35823040 None:intergenic
TGTAAGAAACATCGTGACAA+GGG 0.589365 1.1:+35823271 None:intergenic
AGGAAAGTTGTTCAATGCAA+CGG 0.592106 1.1:+35816967 None:intergenic
GAAAGGCGCTCGTGACTTGG+GGG 0.593148 1.1:+35817798 None:intergenic
GGGTAATTACCGTTAGAAGA+GGG 0.594020 1.1:+35823591 None:intergenic
GCATTCTGCTGAAGGCAGGA+GGG 0.597951 1.1:+35817936 None:intergenic
AGACTCATCATCTACATCAC+AGG 0.601167 1.1:+35817893 None:intergenic
CAGTACACAAACCTAGGGCT+AGG 0.602499 1.1:-35816696 MS.gene073679:CDS
CATCTCTGGTGGTTTCCTGG+TGG 0.603360 1.1:-35823027 MS.gene073679:CDS
ATCATTTCAAATGACCCTGA+AGG 0.604755 1.1:+35820177 None:intergenic
TTAAGTGGGATATTCCATGA+AGG 0.609573 1.1:+35817867 None:intergenic
ATGTAAGAAACATCGTGACA+AGG 0.611925 1.1:+35823270 None:intergenic
GTTGTTGAATGAAGAAGGAG+AGG 0.612306 1.1:+35823620 None:intergenic
ATATACTCATTAAACTTCAA+GGG 0.612812 1.1:+35823294 None:intergenic
ATTCAATTTGCATTAGAGCG+AGG 0.614834 1.1:-35820246 MS.gene073679:CDS
GACATAGTTCGAATATCCAA+GGG 0.618179 1.1:+35820300 None:intergenic
AACAACACTCCCTCTTCTAA+CGG 0.620325 1.1:-35823600 MS.gene073679:intron
TGGAGATGGATCGTCTTGCA+CGG 0.620920 1.1:-35816650 MS.gene073679:CDS
TTGGAGACTGATGAATGTCA+AGG 0.624402 1.1:-35817041 MS.gene073679:CDS
CCGGTCATAAATTCCAGACA+AGG 0.626956 1.1:+35816904 None:intergenic
TTTCCCAGTACACAAACCTA+GGG 0.629461 1.1:-35816701 MS.gene073679:CDS
TTGAAATGAGTACCACCACC+AGG 0.637461 1.1:+35823012 None:intergenic
CCCAGTGTCAGGAATTACCA+AGG 0.646751 1.1:+35823329 None:intergenic
GTACTGGGAAAAGAGGTAGT+TGG 0.648124 1.1:+35816713 None:intergenic
TGAACTCGAATAACAATGGG+AGG 0.652659 1.1:-35823783 None:intergenic
ATCCAAAGGATCATCTCTGG+TGG 0.653009 1.1:-35823038 MS.gene073679:CDS
CATCTTGCTGATGTCAAAGG+AGG 0.661435 1.1:-35823075 MS.gene073679:CDS
TTGTCTGGAATTTATGACCG+GGG 0.671528 1.1:-35816902 MS.gene073679:CDS
AATGTGACTCTACATCCCAA+AGG 0.673432 1.1:+35816267 None:intergenic
GAAAAGTTCCAATCAAACCC+CGG 0.678902 1.1:+35816885 None:intergenic
CTTGGTAATTCCTGACACTG+GGG 0.680113 1.1:-35823328 MS.gene073679:CDS
ACAAGTCAGAAATTCTAATG+CGG 0.682589 1.1:-35817835 MS.gene073679:CDS
CCAGTGTCAGGAATTACCAA+GGG 0.688330 1.1:+35823330 None:intergenic
TGTAAAAGATCATATGTACG+AGG 0.751315 1.1:+35816738 None:intergenic
GAGCATCTGAGTACATACAG+AGG 0.756387 1.1:-35822984 MS.gene073679:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr1.1 gene 35816196 35823790 35816196 ID=MS.gene073679
chr1.1 mRNA 35816196 35823790 35816196 ID=MS.gene073679.t1;Parent=MS.gene073679
chr1.1 exon 35823601 35823790 35823601 ID=MS.gene073679.t1.exon1;Parent=MS.gene073679.t1
chr1.1 CDS 35823601 35823790 35823601 ID=cds.MS.gene073679.t1;Parent=MS.gene073679.t1
chr1.1 exon 35822985 35823399 35822985 ID=MS.gene073679.t1.exon2;Parent=MS.gene073679.t1
chr1.1 CDS 35822985 35823399 35822985 ID=cds.MS.gene073679.t1;Parent=MS.gene073679.t1
chr1.1 exon 35820139 35820428 35820139 ID=MS.gene073679.t1.exon3;Parent=MS.gene073679.t1
chr1.1 CDS 35820139 35820428 35820139 ID=cds.MS.gene073679.t1;Parent=MS.gene073679.t1
chr1.1 exon 35817769 35818155 35817769 ID=MS.gene073679.t1.exon4;Parent=MS.gene073679.t1
chr1.1 CDS 35817769 35818155 35817769 ID=cds.MS.gene073679.t1;Parent=MS.gene073679.t1
chr1.1 exon 35816878 35817124 35816878 ID=MS.gene073679.t1.exon5;Parent=MS.gene073679.t1
chr1.1 CDS 35816878 35817124 35816878 ID=cds.MS.gene073679.t1;Parent=MS.gene073679.t1
chr1.1 exon 35816644 35816782 35816644 ID=MS.gene073679.t1.exon6;Parent=MS.gene073679.t1
chr1.1 CDS 35816644 35816782 35816644 ID=cds.MS.gene073679.t1;Parent=MS.gene073679.t1
chr1.1 exon 35816196 35816354 35816196 ID=MS.gene073679.t1.exon7;Parent=MS.gene073679.t1
chr1.1 CDS 35816196 35816354 35816196 ID=cds.MS.gene073679.t1;Parent=MS.gene073679.t1
Gene Sequence

>MS.gene073679

ATGGGAGGTTTTGCTACCATTGGTTCTGCCTTTGATTCTAAATCTGGCCAAATCATAATGGCTTCTTTACTTCTCATGATTGTTTCCTTTTATGTCGGTACTCTTTTTGGTAACAATACTCCTCTTTACATCTCTCACCTTCCTTCTTCCTCTCCTTCTTCATTCAACAACAACACTCCCTCTTCTAACGGTAATTACCCAATTTCATATCAATTTCATTTAATATCTATGAAGCACGGATACGACACCAACACATATAATTACACTGAGTTACGTGATTTTCTCAAATTATTAGCGGTTCTAGTGTGTCAATGTTTGTGTCGTGTTTGGTGTCCGTGTTCGTCCTTCAAATATGCTACTTTCTGACTCACTCATATGCAAATTTTTACAGGAACTTCACGATTTAGTAACAAAGTTGCTCTCACTTACCGCAAAATTCCCTTGGTAATTCCTGACACTGGGGTAGATATATGTCCCTTGAAGTTTAATGAGTATATCCCTTGTCACGATGTTTCTTACATTGCAACATTGATGTCTCATCTTGATGTTTCTAGAAAACAAGAGCTGGAGAGACACTGTCCTCCACTCGAAAACCGTTTGTTTTGTTTGGTTCCGCCGCCTAAGGATTATAAAATACCTATCAGATGGCCTCTTAGTAGGGATTATGTTTGGAGGAGTAATGTGAATCATACACATCTTGCTGATGTCAAAGGAGGCCAAAATTGGGTCCATCCAAAGGATCATCTCTGGTGGTTTCCTGGTGGTGGTACTCATTTCAAGAATGGAGCATCTGAGTACATACAGAGGTACCGACCTATATACTTCTTTGGATGATTGGAACTAATATGATTGATTACAACTTCATTTTTTTTTTATGCGTTGGATTAGTTGTAAGACTAGACATGATATGATTTGTAATGACAACATTAGAAAGAGAGAGAGAGTTCGGGTAACACCTATAGTAGAAAAGAGAGTGGAAACCAGGCTTAAGTGGTTTGAGCATGTATAGAGAAAACTTGTAGATTTTGTAGTAAGGAGGGTAGTTAGGTCACTAGAGGCAGAGGTAGACCTAGAAAAACTTTAAGAGACATTGTTAGGGAAGATTTAGAGATTAATGAGTTGGATTCAAATATGGTTTATAATAGAATCTTATTGCGTAATTTGATCCATGTAGCCGACCCCATTTAGTGGGATAAGGCTTGGTTGTTGCTGTTGAATTCTTGCGTAAGACTAGACATGACAGGGTTAGAAATGACAACATTAGAGAGTTGTGGTAGCACCTATAGTAAAAAAGATGGGGGAAACTAGGTTTAGTTGATTTGGGCATGTAGAGAGAAAACCTGTAGATTATGTAGTAAGGAGAGCAAATCAGATGGAGATAGTCAGATCACTAAAGGTAGAGGTAGACTTAGAAAAAATTTAGAGATTAATGAGTTGGATTCAAATATGGTTTATGATAGAACATTATTGCGTAATTTGATCAATGTAGCCGACCCAACTTAGTGGGATAAGGCTTGATTGTTTGCTGCATGAATTCTTGCGCGAGATAAATATTTTGAAATCAGATTGAGTGTGGCTCAATGTTGTCAAACACGGTGATACTATGGCGGAGTGCGGTGATGGAATTTTTTACAACCACCATGGCCAACATGTGAAAGATGGCACCGCCATGGCATTTTGTGATATGCGAACATATGATCTATTTCATATTTGAAGTAGATTCTTCTATAAATTAAGATTGAGATAACTATGTGAGAGAGCAGGTAAAGATGAGTAGAGTAATGCCAGATGTAAATTTTTATCTCTTTTTGATAGTAATATATTTATACCCTGTGATTTGGGTCTACTGAGAATTCCTTATCTCGAGCTAGGTTCTTCTGCAAATAATTTGTGCATTTGTTTATTGAGCTTAGATTTTCTTCTTTTGCAATTACATGTTATGCTTTATTTCTTTGGAAAACTAAATAATCTTTTTACCCTGATCGTATTGAATTATTTGGAAATTATTCTTAAGATAATATGTGACAAATTGATTGGTGTTGAATGGAAAATGACGTGACTGTCTGATTTTTAATTGTCTTTCAAAGGTGTAATCATATGGTCACCAAGCAAACTGCGACTCACCTGTTATTTTGTTTAAAACATTAGATGGGAGAAAAGACAATGAAAGGAGAAATATTGTTTGAAATTGAAATTAAGAGAAATTTTTGGTCTTCAATTTCTGTTCAAATAATTAAAAGTTTCAAACTTTAAAGCTTTGAGAGTTAGGAGGGGATGGCTTTGGAGGAAAGCTAGGGAGGGAGGCTAAACACCCGTATATGGGGGAACTAAAATAAAAATGCGAGGAGGACTTTGCCGGGTTTAAAAGAAACACCGAACTCATTCCGTCCCCTCCTTCTCTTTCTAAAAAGTCTAAAACAAGGGGATAACTTATCTTAAATTCTCTTTCGTCCTTCTCTCCTCTCACCTCCAGGCACCCATCCCAAACCAAGCCTTAAGATCTTCTTTTCTTTCTATTTTTTTTTCTCAATCTAAACCTAGTGCTAGCGCTCCCTGAGCCTTGAGGCCTGCCCTGACCCTGGTCAGTCTCTGGGACCCACTCACATAAACTTCTCTCGTTGTTAAATCCAAAAAAAAAAAAAAAAACTAGTGTTAAGAAGTTAAAGTAACTATGTGAAATCCAGAAGATAATCAGTCACGGCTGACCACAGAAACTTCTTTCCTTATGTTTCTGAATTCTGAGACATCTGTTTCGAGTACGATTTCCAATAAAAGAAAGTGTTATAAAAAAAAATTATGTTACAACGTTGTAGTAAAGAGGTACGCGCACAATAGACTAAAATTATTGGAGTCAATGGAACCACAAGGGTGTGTTTATTTTGAAAATGGTGCCTCAGGAGTCAACTTAATGAAGGAAAAGTTGTGGTTGGCACATTGGAATGAATCCCTTTTCTCTCTGGCTTACTTTTGGACTTATGGGCTTTATACAAAAATCATAGTAAAAAATTGAATGCTGTTTGTAGATACTACCTATTTCGGGCCATTGCTTTGTTGCAATTTGTAGTTAGTTTGTGCTATTGAAACAGGCTGAAACCAGATGCAGCTAACTGATATTTTTAAGGTTACATACACCGGTGAGAGGGAGAGAGGGGAGGGAAAGCTAATCTTGAGTCTTGACATTTTATTCATACATAAATGGTCAATATCAGTTTCAACTTTACTTGAAAGTTTTAAGTAAAATCAATTTCTACCTATCTTGCAATTATTTTGCTTGGTGCTATAACTTGAGCATGGAATGTTTGTATGTTGTGTGAGTAGTGCAAGATACAACTGTTAGTCTTTTGATGGCTTTACAAATGCAGGATAGGAAATATGATTACAAATGAGACTGGTGATTTACGTTCTGCGGGCATAGTTCAAGTTCTGGATCTTGGCTGTGGTGTGGCCAGCTTTTCAGCGTATCTTCTTCCCTTGGATATTCGAACTATGTCTTTTGCTCCCAAGGATGGTCATGAAAACCAGATTCAATTTGCATTAGAGCGAGGAATAGGTGCAATGATCTCCGCCATGTCCACAAAACAACTGCCATTTCCTTCAGGGTCATTTGAAATGATTCACTGTTCAAGATGCCGTATAGACTTTCATGAAAATGGTAATTGGCTTTCCTTATGATGTTTATATATATTGGCTTTCCTTTCGAGAGGAGGGAAATTGAGTGGAGAGAAACTGAGAGGAAGTTTTGTTTACTTTTGTTTCATTGCAAAATAACACAAGAAAGGAAAAATTGGTGTGGGACACACCTCTCGCCAAGTGGAGAGGTAACACCAATTTCAAGTAGACATTCACTCTTTCCAGCTATACCAATCTAATTATCTATCCTCTGTTGTGGGGTTCACTTGATTCTTCTTATTTTGTCAAGGGTAAATATGACATTATATCAATTATTCATTTTCTTAATTTTCATTTTAATTGTTGTCCAACCAAACAATTATAGAGAAAATTGAACTTTTCTCTGCACCTAAACGAAGCTTTGGTTGACAACTAGAATGTGAACTTATTCGTAGAGTAAAATGGTATTTTGTCTAGTTTCGAAATGTTTTTAGCTCTCATTTGTTTATGATCTTTAATTCCTTTGGCACACTTCTTGAACGCAGTGGGTGATCTCCTCGACCCTTGCATTGCCGTAACTAGGTTCCTGCTACTTCAACACAGGGTTCTGCATATTCTTAAGCTTGAAATTCACTCCTTGAAATGATTGCTATAATTTTCCATTTCTTTCCCGGTGAGAGTTCTGGCAGATCTAACCAATTTGTTAGGGACCAAGAATTAGGATGGAAATAAAGAATCTTAATATTAAAAAATTAAAAGAATATTAATAATATAATTCTGCCCTTTGGGTTCTCCTTTACAAAAGATCTCTCCTTTTCCCTCTCATATCAAATGCACAGTATTCTATTTTCTTACTGTATTTCTCCTATTTATATATGATACATCTTATCTAATTGACCAACTACTGACTAATCCTCATTACTACCCTTTATCTTTATCCTAACAGCTTGATGTTAAACGTGTTTGATTCAGTCCTTTTTTTTTTGAAAATCTTATTTATTGTTAATACATGCCTGATATAGCTAAAAGAAGTACTTTTGTGTTTACAAATTGTGATATTTTCTAAAACCAAAATGTTGGACAGCTTTCCATTTATGTATCACTGCTGTGCTCCAAAGTTGTTTAATTAGTTTGTGTTTTACCATAGTGAAATTTACTAATAACATTGATCTCAAGCCAACCAATGCATTGCAACACGTTAGAAGTATGTTTTGTCTCTTCTTAAGATTGACTTCCATTACCATGTTTGAAATCTGTCCCTGACCATTTCTTTTTTGCTACCGTTCTACCGCAAAGCATTCATTTATCTGACGAGCGATGAGTGACATTTTAACATATCTCTTTACTTTTGTTTGTGCAAAATACCATTTTATGCTATTGCTCCCTTGTTCAAGAAGTATATTTTTGTTATATGATACTTTAAAAAAGTTGTGTATTTTTAAGGATAAAAGGAAATACCCTCCCTCAACTATAGGTAATTTTACAAAACATGTGTTGGAATCTTATTAAACTAGCTCCCTAAAAACATGTTTGTAAAGTTAACTTTTATGTATGTATTGAGTTGTTAGATTTTGAACCATAAATTTTTTTTATATTTATTTTTTTTTTAATTTTTTTTAGAATTGAGCTTTGTTGAAGTTATTTCTTGCATTTTGTCTAGTTTTTAATAATTCATCTGTAAGGATATATCATCAAGAGAAAATAAAACAGAAAACAACAAAACAAGAAGTACTGGCATCATTATTCTCATTGTAGTTTTGAATTGTATAGCTTAGAGGTTTTCTTTTTCCCTTATTAGCTATCATCATCATCATCATCATTTTTGTTTTTATTGAATGACCTGGTACAATATGTTCCAAAGGCTGCTATAAACACTGATGGGTGAACCCTTTGCCCAATTAGACCCATTTATTATTTCTGTTATAGATTCAATCTGACTATCTTATAGAATAGATGAAATTTGTATATGTCAAAGCTATTATTGTTGGTGGGGTTGTTTGTTGCTAATTTATTTTGTTATACGCAGATGGGATTTTGCTGAAGGAATTGGATCGTCTTCTTCGCTCAAATGGTTATTTTGTTTATTCAGCACCACCTGCTTACAGAAAAGATAAAGATTTTCCTGTCATTTGGGATAAGTTGGTTAACTTGACTACAGCAATGTGTTGGAGACTCATTGCTCGTAAAGTTCAGACTGCAATATGGATTAAAGAAAATAATCATCCCTCCTGCCTTCAGCAGAATGCAGAACAAAAGCTTATAAATGCCTGTGATGTAGATGATGAGTCTAAACCTTCATGGAATATCCCACTTAAGAACTGTATACAAGTCAGAAATTCTAATGCGGAATCTTACAAGCTACCCCCAAGTCACGAGCGCCTTTCAGTGTTTTCTGAAAAGCTGAACAAGATAGGTCTGCCAATTCTCTTTTGATATACTGTGCAAGAAGTTAATGTCCATTTTTCTTATTCTTAAATTATTCTTACCAATCTTGATGTGCACACTTACACCATAGATTTTTTGTATGCACATAATATAATTGTGTTGCAAATCTCAGATAGCATCATATACCATGCATGCTCTCAGAAATTTCATGAACTGTGATTCCAATGGTATTGCTAATGATACATATGCTTTATTATCATCATAACGATTTCATTAATCAAAGGCACAATCTTCACTAGAGCAGATATTAGCTTTGCTAAGATCATGTTTTGGTATATGATGCACAGCAGATATTAGCTTTTCTAAGATCACGTTTCTGAAAAAATTTGGCGTATTATCATAAGGTATATAGTTGAACACCTCCCGTAATTGCCTACGATATGTGTTTTCTTTTTAGTTCATTTACATACTGATATCAATATAGAAAGTCAATATCTTGTTTGTGCTCTGTTTTTTAGTTGAGATTGGAAAGGATCAATACATTTAAATCAAAAGGACGATATTGGACAACTGTTCAACTGGAAATCATAAGATAAAATAAACATGAATGTGGTGCACACAGAATTCCCTTTCAATCTAATGTGATGACCCTTCTAACCTTTTAAACAACAGGTATAAATCGAGATGAATTTACATCAGATTCTATCTTCTGGCAAGATCAGACTCGTCATTATTGGAGACTGATGAATGTCAAGGAAACAGAAATACGCAACGTGATGGATATGAATGCCTTTTATGGTGGATTTGCCGTTGCATTGAACAACTTTCCTGTTTGGGTAATGAATGTAGTTCCTGCAGGCATGAAGAACACCTTGTCTGGAATTTATGACCGGGGTTTGATTGGAACTTTTCATGATTGGTAATATTTTGCAACGAATATCATAAATAGTAAATACAATGTTAAAATGTGTATTATTAGTTGACTGCAGCTAATGTAGATATCTTTTACATCAGGTGTGAGCCATTTTCAAGTTATCCTCGTACATATGATCTTTTACATGCCAACTACCTCTTTTCCCAGTACACAAACCTAGGGCTAGGTTGTATATTAGAGGATATCATGCTGGAGATGGATCGTCTTGCACGGCCACTGGTAATTAATTACTTTTTTAAATGTTACCTTCATGGTTCTCTGCATGTATTATTGAAGTCGAAGTTCATGTGACTGAAATAAAATAAGAATTTTGGTAAATTCTGATATACCAAAATACATCGGTTATTCAATGAATCTAAAAAGTGAATTGACTCTTATAATAAGGAACGGATGTAGTACAAAGTTTTGTCAAATAGCGGCTATAGCTACACTATAGCAGTGCAACATAGTGTTTATTTGTCTTCCTGTGGATAAAGTTGATAACTTAGAGCTCACTTATTATCTGCAGGGATTTATTATCATTAGGGATGAAGAACATATTATATCAAGAATCAACGATTTAGCCCCAAAATTCCTTTGGGATGTAGAGTCACATTTGCTAGAGAACAAAGAAAAGAAGATGGAAACTGTTTTAATTTGTAGAAAAAAGTTTTGGGCAATCCTCTAA
Protein sequence

>MS.gene073679.t1

MGGFATIGSAFDSKSGQIIMASLLLMIVSFYVGTLFGNNTPLYISHLPSSSPSSFNNNTPSSNGTSRFSNKVALTYRKIPLVIPDTGVDICPLKFNEYIPCHDVSYIATLMSHLDVSRKQELERHCPPLENRLFCLVPPPKDYKIPIRWPLSRDYVWRSNVNHTHLADVKGGQNWVHPKDHLWWFPGGGTHFKNGASEYIQRIGNMITNETGDLRSAGIVQVLDLGCGVASFSAYLLPLDIRTMSFAPKDGHENQIQFALERGIGAMISAMSTKQLPFPSGSFEMIHCSRCRIDFHENDGILLKELDRLLRSNGYFVYSAPPAYRKDKDFPVIWDKLVNLTTAMCWRLIARKVQTAIWIKENNHPSCLQQNAEQKLINACDVDDESKPSWNIPLKNCIQVRNSNAESYKLPPSHERLSVFSEKLNKIGINRDEFTSDSIFWQDQTRHYWRLMNVKETEIRNVMDMNAFYGGFAVALNNFPVWVMNVVPAGMKNTLSGIYDRGLIGTFHDWCEPFSSYPRTYDLLHANYLFSQYTNLGLGCILEDIMLEMDRLARPLGFIIIRDEEHIISRINDLAPKFLWDVESHLLENKEKKMETVLICRKKFWAIL