AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene84077

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene84077.t1 MTR_1g051055 98.191 608 9 1 1 608 1 606 0.0 1246
MS.gene84077.t1 MTR_1g069785 46.396 541 263 9 81 605 56 585 2.41e-158 467
MS.gene84077.t1 MTR_1g442840 41.176 612 342 8 8 605 8 615 4.40e-158 467
MS.gene84077.t1 MTR_1g442840 46.108 501 258 6 114 605 5 502 4.08e-153 451
MS.gene84077.t1 MTR_1g075900 45.143 525 276 6 90 605 93 614 8.56e-151 449
MS.gene84077.t1 MTR_5g080720 44.061 522 274 7 89 602 126 637 2.33e-145 435
MS.gene84077.t1 MTR_3g068990 43.570 521 276 7 90 602 155 665 2.62e-138 418
MS.gene84077.t1 MTR_8g432620 40.377 530 291 8 88 607 88 602 1.03e-137 414
MS.gene84077.t1 MTR_7g076150 40.755 530 289 9 88 607 75 589 1.27e-136 411
MS.gene84077.t1 MTR_5g080720 46.563 451 226 6 89 531 126 569 1.34e-135 408
MS.gene84077.t1 MTR_1g029730 42.366 524 285 9 90 604 143 658 2.89e-134 407
MS.gene84077.t1 MTR_3g088790 43.148 540 248 18 96 605 232 742 1.11e-129 398
MS.gene84077.t1 MTR_8g100075 38.577 534 297 9 91 606 101 621 2.03e-129 394
MS.gene84077.t1 MTR_1g101370 41.791 536 280 10 91 606 95 618 4.32e-128 390
MS.gene84077.t1 MTR_7g076170 38.235 544 297 10 86 606 99 626 6.38e-127 387
MS.gene84077.t1 MTR_0019s0120 40.074 539 281 12 91 606 108 627 4.94e-126 385
MS.gene84077.t1 MTR_5g040360 39.057 530 291 11 91 605 87 599 2.41e-124 380
MS.gene84077.t1 MTR_8g071280 39.698 529 292 10 91 605 73 588 3.77e-124 379
MS.gene84077.t1 MTR_3g040370 39.130 529 289 9 91 605 88 597 3.00e-123 377
MS.gene84077.t1 MTR_8g055840 38.752 529 295 10 91 605 87 600 5.30e-123 377
MS.gene84077.t1 MTR_8g432680 34.823 649 355 15 4 606 5 631 5.89e-123 377
MS.gene84077.t1 MTR_5g083150 40.462 519 278 11 97 605 101 598 3.71e-122 374
MS.gene84077.t1 MTR_8g093880 38.726 581 316 17 47 605 244 806 1.25e-121 379
MS.gene84077.t1 MTR_4g098890 40.613 522 266 11 91 591 89 587 3.11e-121 393
MS.gene84077.t1 MTR_8g076650 41.379 522 275 13 97 605 387 890 4.45e-120 377
MS.gene84077.t1 MTR_5g026930 38.308 603 315 15 23 605 25 590 1.77e-119 367
MS.gene84077.t1 MTR_5g021100 39.024 574 304 15 49 605 289 833 3.02e-119 374
MS.gene84077.t1 MTR_3g096480 37.755 588 310 17 47 605 216 776 4.61e-117 367
MS.gene84077.t1 MTR_3g071530 38.596 513 292 7 97 605 153 646 1.37e-116 361
MS.gene84077.t1 MTR_7g006060 39.429 525 280 12 80 581 35 544 1.90e-116 358
MS.gene84077.t1 MTR_7g076150 41.538 455 245 8 163 607 3 446 3.46e-116 353
MS.gene84077.t1 MTR_0019s0120 39.729 443 228 11 91 510 108 534 1.02e-97 308
MS.gene84077.t1 MTR_3g074780 34.799 546 317 11 88 605 154 688 2.06e-96 310
MS.gene84077.t1 MTR_4g083030 33.119 622 336 19 23 604 22 603 3.11e-96 307
MS.gene84077.t1 MTR_5g064080 35.165 546 305 12 88 605 146 670 6.90e-94 303
MS.gene84077.t1 MTR_5g026930 37.795 508 263 13 23 510 25 499 6.61e-93 295
MS.gene84077.t1 MTR_1g022255 32.625 518 311 12 98 605 210 699 2.24e-86 283
MS.gene84077.t1 MTR_1g022255 29.427 384 239 10 98 471 210 571 2.80e-46 172
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene84077.t1 AT3G10200 68.614 599 169 6 13 608 9 591 0.0 896
MS.gene84077.t1 AT5G04060 68.144 609 181 5 2 608 3 600 0.0 893
MS.gene84077.t1 AT3G10200 68.382 544 154 5 14 555 10 537 0.0 815
MS.gene84077.t1 AT1G04430 45.336 536 265 7 90 605 86 613 8.15e-155 459
MS.gene84077.t1 AT1G04430 45.336 536 265 7 90 605 86 613 8.15e-155 459
MS.gene84077.t1 AT1G04430 45.336 536 265 7 90 605 86 613 8.15e-155 459
MS.gene84077.t1 AT3G23300 45.038 524 277 7 90 605 81 601 2.96e-151 449
MS.gene84077.t1 AT3G23300 45.038 524 277 7 90 605 81 601 2.96e-151 449
MS.gene84077.t1 AT5G14430 43.866 595 309 9 24 607 26 606 8.44e-151 448
MS.gene84077.t1 AT4G14360 44.466 524 280 6 90 605 78 598 1.69e-149 445
MS.gene84077.t1 AT4G14360 44.466 524 280 6 90 605 78 598 1.69e-149 445
MS.gene84077.t1 AT5G14430 43.529 595 311 9 24 607 26 606 3.15e-149 444
MS.gene84077.t1 AT5G06050 42.857 525 280 8 89 604 150 663 3.18e-141 426
MS.gene84077.t1 AT1G77260 42.777 533 282 9 84 604 134 655 3.83e-140 422
MS.gene84077.t1 AT2G39750 39.613 568 318 7 44 604 142 691 1.71e-139 422
MS.gene84077.t1 AT4G19120 37.377 610 332 11 14 605 13 590 1.94e-130 395
MS.gene84077.t1 AT4G19120 37.377 610 332 11 14 605 13 590 1.94e-130 395
MS.gene84077.t1 AT4G00740 39.888 534 288 10 88 607 81 595 1.06e-129 394
MS.gene84077.t1 AT4G00740 39.888 534 288 10 88 607 81 595 1.06e-129 394
MS.gene84077.t1 AT1G26850 39.179 536 289 10 91 605 89 608 1.89e-127 388
MS.gene84077.t1 AT1G26850 39.179 536 289 10 91 605 89 608 1.89e-127 388
MS.gene84077.t1 AT4G10440 37.823 542 291 10 91 606 96 617 1.73e-126 386
MS.gene84077.t1 AT1G29470 40.569 562 300 16 61 605 213 757 6.75e-126 389
MS.gene84077.t1 AT1G29470 40.569 562 300 16 61 605 213 757 6.75e-126 389
MS.gene84077.t1 AT1G33170 37.950 556 307 12 75 606 94 635 8.30e-126 385
MS.gene84077.t1 AT2G45750 38.698 553 302 11 80 607 77 617 1.05e-125 384
MS.gene84077.t1 AT2G34300 41.856 528 268 14 97 605 250 757 1.13e-124 386
MS.gene84077.t1 AT2G34300 41.856 528 268 14 97 605 250 757 1.13e-124 386
MS.gene84077.t1 AT2G34300 41.856 528 268 14 97 605 250 757 1.13e-124 386
MS.gene84077.t1 AT1G31850 38.971 544 299 12 80 605 68 596 2.75e-124 380
MS.gene84077.t1 AT1G31850 38.971 544 299 12 80 605 68 596 2.75e-124 380
MS.gene84077.t1 AT1G31850 38.971 544 299 12 80 605 68 596 2.75e-124 380
MS.gene84077.t1 AT1G31850 38.971 544 299 12 80 605 68 596 2.75e-124 380
MS.gene84077.t1 AT2G40280 40.116 516 278 14 97 605 90 581 1.34e-122 375
MS.gene84077.t1 AT2G43200 40.720 528 280 12 90 599 93 605 2.23e-122 375
MS.gene84077.t1 AT2G43200 40.720 528 280 12 90 599 93 605 2.23e-122 375
MS.gene84077.t1 AT4G00750 37.415 588 323 12 45 606 55 623 6.71e-122 374
MS.gene84077.t1 AT3G56080 38.667 600 304 17 18 580 19 591 1.36e-121 373
MS.gene84077.t1 AT5G64030 40.458 524 282 13 97 605 308 816 3.30e-121 379
MS.gene84077.t1 AT3G51070 39.086 591 311 17 46 605 317 889 3.77e-121 380
MS.gene84077.t1 AT4G18030 38.649 533 292 10 91 605 88 603 1.16e-120 371
MS.gene84077.t1 AT3G56080 43.545 457 219 13 97 535 114 549 1.40e-111 345
MS.gene84077.t1 AT3G51070 39.575 518 271 14 46 532 317 823 3.25e-106 339
MS.gene84077.t1 AT4G19120 36.505 515 281 9 14 510 13 499 2.24e-102 320
MS.gene84077.t1 AT1G13860 34.559 544 297 15 90 602 83 598 1.34e-95 305
MS.gene84077.t1 AT1G13860 34.559 544 297 15 90 602 83 598 1.34e-95 305
MS.gene84077.t1 AT1G13860 34.559 544 297 15 90 602 83 598 1.34e-95 305
MS.gene84077.t1 AT1G13860 34.559 544 297 15 90 602 83 598 1.34e-95 305
MS.gene84077.t1 AT1G78240 33.824 544 322 11 88 604 146 678 8.18e-95 305
MS.gene84077.t1 AT1G78240 33.824 544 322 11 88 604 146 678 8.18e-95 305
MS.gene84077.t1 AT2G03480 32.062 577 307 15 67 604 62 592 2.96e-91 293
MS.gene84077.t1 AT1G19430 34.489 519 301 14 98 605 232 722 1.33e-89 293
MS.gene84077.t1 AT1G19430 34.489 519 301 14 98 605 232 722 1.33e-89 293
MS.gene84077.t1 AT1G26850 36.449 428 239 8 91 498 89 503 1.26e-88 284
MS.gene84077.t1 AT2G03480 31.716 577 320 15 67 604 62 603 3.12e-88 286
MS.gene84077.t1 AT1G13860 36.239 436 239 12 194 602 19 442 4.45e-81 263
MS.gene84077.t1 AT2G03480 31.564 358 208 10 67 404 62 402 7.54e-50 179
MS.gene84077.t1 AT1G19430 31.688 385 232 11 98 472 232 595 5.53e-49 180

Find 128 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTACATCCCAAAGGAATTT+TGG 0.124701 1.4:+37529407 None:intergenic
CCTTTGGATGGACCCAATTT+TGG 0.176094 1.4:+37536059 None:intergenic
CGTATAGACTTTCATGAAAA+TGG 0.234122 1.4:-37533402 MS.gene84077:intron
ATGTCAAAGGAGGCCAAAAT+TGG 0.261908 1.4:-37536072 MS.gene84077:CDS
AACGGCAAATCCACCATAAA+AGG 0.279828 1.4:+37530117 None:intergenic
GATCATCTCTGGTGGTTTCC+TGG 0.288695 1.4:-37536037 MS.gene84077:CDS
TCTGCCTTTGATTCTAAATC+TGG 0.293833 1.4:-37536743 MS.gene84077:CDS
GTACGAGGATAACTTGAAAA+TGG 0.319695 1.4:+37529885 None:intergenic
AAACAACTGCCATTTCCTTC+AGG 0.326414 1.4:-37533456 MS.gene84077:CDS
CATTGAACAACTTTCCTGTT+TGG 0.339170 1.4:-37530093 MS.gene84077:CDS
TGCAGGGATTTATTATCATT+AGG 0.340512 1.4:-37529469 MS.gene84077:intron
GCAGGGATTTATTATCATTA+GGG 0.340665 1.4:-37529468 MS.gene84077:intron
AAGGTGTTCTTCATGCTTGC+AGG 0.342084 1.4:+37530055 None:intergenic
CTAGCCCAAGGTTTGTATAC+TGG 0.346501 1.4:+37529829 None:intergenic
GCTCTAATGCAAATTGAATC+TGG 0.351997 1.4:+37533514 None:intergenic
GTAAAGAAGCCATTATGATT+TGG 0.353865 1.4:+37536720 None:intergenic
ATTGAACAACTTTCCTGTTT+GGG 0.357492 1.4:-37530092 MS.gene84077:CDS
TGCGGGCATAGTTCAAGTTC+TGG 0.360065 1.4:-37533628 MS.gene84077:CDS
CTATCAGATGGCCTCTTAGT+AGG 0.363179 1.4:-37536138 MS.gene84077:CDS
CTCTTAGTAGGGATTATGTT+TGG 0.366295 1.4:-37536126 MS.gene84077:CDS
AAGATCAGACTCGTCATTAT+TGG 0.376187 1.4:-37530192 MS.gene84077:CDS
ATTTATGACCGGGGTTTGAT+TGG 0.378010 1.4:-37530025 MS.gene84077:CDS
ATAGTTCAAGTTCTGGATCT+TGG 0.379508 1.4:-37533621 MS.gene84077:CDS
ATTTAGCCCCAAAATTCCTT+TGG 0.384533 1.4:-37529415 MS.gene84077:CDS
TTACGTCAGATTCTATCTTC+TGG 0.386854 1.4:-37530216 MS.gene84077:CDS
TTTGCATTAGAGCGAGGAAT+TGG 0.395270 1.4:-37533504 MS.gene84077:CDS
AGCATGAAGAACACCTTGTC+TGG 0.400833 1.4:-37530049 MS.gene84077:CDS
TAAATCTGGCCAAATCATAA+TGG 0.406380 1.4:-37536729 MS.gene84077:CDS
TCAAATGACCCTGAAGGAAA+TGG 0.422002 1.4:+37533447 None:intergenic
TCCTGTCATTTGGGATAAGT+TGG 0.422589 1.4:-37531281 MS.gene84077:CDS
TTTAGCCCCAAAATTCCTTT+GGG 0.425741 1.4:-37529414 MS.gene84077:CDS
GTAAAGTTCAGACTGCAATA+TGG 0.426050 1.4:-37531219 MS.gene84077:CDS
ACTGAAAGGCGCTCGTGACT+TGG 0.432443 1.4:+37531037 None:intergenic
TTCAGCGTATCTTCTTCCCT+TGG 0.432592 1.4:-37533580 MS.gene84077:CDS
ACTACATTCATTACCCAAAC+AGG 0.436158 1.4:+37530079 None:intergenic
CTGAAAGGCGCTCGTGACTT+GGG 0.436945 1.4:+37531038 None:intergenic
TTGTTTGGTTCCGCCGCCTA+AGG 0.437102 1.4:-37536173 MS.gene84077:CDS
TCTGAAAAGCTGAACAAGAT+AGG 0.447938 1.4:-37531010 MS.gene84077:intron
CTACTAAGAGGCCATCTGAT+AGG 0.448095 1.4:+37536139 None:intergenic
GATCGCCTTCTTCGCTCAAA+TGG 0.451283 1.4:-37531352 MS.gene84077:CDS
TTCTGCATTCTGCAGAAGGC+AGG 0.455660 1.4:+37531174 None:intergenic
AACAACTGCCATTTCCTTCA+GGG 0.458335 1.4:-37533455 MS.gene84077:CDS
ACTTGTATACAGTTCTTAAG+TGG 0.464593 1.4:+37531094 None:intergenic
CATATATCTACCCCAGTGTC+AGG 0.465947 1.4:+37536325 None:intergenic
CTCTGGTGGTTTCCTGGTGG+TGG 0.466744 1.4:-37536031 MS.gene84077:CDS
TTTCCCAGTATACAAACCTT+GGG 0.469268 1.4:-37529833 MS.gene84077:CDS
GATATACTCGTTAAACTTCA+AGG 0.469296 1.4:+37536300 None:intergenic
AACCACCAGAGATGATCCTT+TGG 0.471865 1.4:+37536043 None:intergenic
CACTTACCGCAAAATTCCCT+TGG 0.471979 1.4:-37536353 MS.gene84077:CDS
CCTTGGTAATTCCTGACACT+GGG 0.480156 1.4:-37536336 MS.gene84077:CDS
AGAAGATATCATGCTGGAGA+TGG 0.484931 1.4:-37529796 MS.gene84077:CDS
AATAACCATTTGAGCGAAGA+AGG 0.485095 1.4:+37531347 None:intergenic
GGATCGTCTTGCACGGCCAC+TGG 0.497371 1.4:-37529775 MS.gene84077:intron
CAGTATACAAACCTTGGGCT+AGG 0.498511 1.4:-37529828 MS.gene84077:CDS
AAGAAGATACGCTGAAAAGC+TGG 0.500896 1.4:+37533587 None:intergenic
AGAGAACAAAGAAAAGAAGA+TGG 0.501740 1.4:-37529372 MS.gene84077:CDS
TATCAGATGGCCTCTTAGTA+GGG 0.502303 1.4:-37536137 MS.gene84077:CDS
GGAAGGTGAGAGATGTAAAG+AGG 0.503623 1.4:+37536647 None:intergenic
TCCATCCAAAGGATCATCTC+TGG 0.505245 1.4:-37536048 MS.gene84077:CDS
AATGAAGAAGGAGAGGAAGA+AGG 0.507870 1.4:+37536626 None:intergenic
TAGCCCAAGGTTTGTATACT+GGG 0.510327 1.4:+37529830 None:intergenic
CCCTTGGTAATTCCTGACAC+TGG 0.510885 1.4:-37536337 MS.gene84077:CDS
CAAGTTCTGGATCTTGGCTG+TGG 0.512542 1.4:-37533615 MS.gene84077:CDS
TTAGTAGGGATTATGTTTGG+AGG 0.514597 1.4:-37536123 MS.gene84077:CDS
CCTTGTCTGGAATTTATGAC+CGG 0.514633 1.4:-37530036 MS.gene84077:CDS
AAGAAGGAGAGGAAGAAGGA+AGG 0.516792 1.4:+37536630 None:intergenic
TTTGTTCTGCATTCTGCAGA+AGG 0.520371 1.4:+37531170 None:intergenic
GGTGGTACTCATTTCAAGAA+TGG 0.526261 1.4:-37536013 MS.gene84077:CDS
CAAACATAATCCCTACTAAG+AGG 0.526307 1.4:+37536127 None:intergenic
TGTCAAAGGAGGCCAAAATT+GGG 0.535636 1.4:-37536071 MS.gene84077:CDS
ACGGGTGATTTACGTTCTGC+GGG 0.539157 1.4:-37533645 MS.gene84077:CDS
TGTTTCTAGAAAACAAGAGC+TGG 0.539783 1.4:-37536230 MS.gene84077:CDS
GTGTTGTTGTTGAATGAAGA+AGG 0.541421 1.4:+37536614 None:intergenic
GACGGGTGATTTACGTTCTG+CGG 0.545323 1.4:-37533646 MS.gene84077:CDS
GCGGAACCAAACAAAACAAA+CGG 0.549143 1.4:+37536182 None:intergenic
CTTGTATACAGTTCTTAAGT+GGG 0.549205 1.4:+37531095 None:intergenic
TTGGCCAGATTTAGAATCAA+AGG 0.550068 1.4:+37536739 None:intergenic
GGTTTGTATACTGGGAAAAG+AGG 0.551310 1.4:+37529838 None:intergenic
TGAAAGGCGCTCGTGACTTG+GGG 0.551470 1.4:+37531039 None:intergenic
AGACATAGTTCGAATATCCA+AGG 0.552080 1.4:+37533563 None:intergenic
TGCATTCTGCAGAAGGCAGG+AGG 0.553947 1.4:+37531177 None:intergenic
CCAAAATTGGGTCCATCCAA+AGG 0.558669 1.4:-37536059 MS.gene84077:CDS
ATGATGAGTCTAAACCTTCG+TGG 0.564064 1.4:-37531123 MS.gene84077:CDS
ACCAACTTATCCCAAATGAC+AGG 0.566105 1.4:+37531280 None:intergenic
AATATGATTACAAATGAGAC+GGG 0.568092 1.4:-37533663 MS.gene84077:CDS
TGGGTAATTACCGTTAGAAG+AGG 0.569025 1.4:+37536589 None:intergenic
AACAGAAATACGCAACGTGA+TGG 0.574714 1.4:-37530149 MS.gene84077:CDS
ATTATAAAATACCTATCAGA+TGG 0.575123 1.4:-37536150 MS.gene84077:CDS
CTTGTCTGGAATTTATGACC+GGG 0.580796 1.4:-37530035 MS.gene84077:CDS
AAATATGATTACAAATGAGA+CGG 0.584704 1.4:-37533664 MS.gene84077:CDS
TCTGGATCTTGGCTGTGGTG+TGG 0.587155 1.4:-37533610 MS.gene84077:CDS
ACACATCTTGCTGATGTCAA+AGG 0.587482 1.4:-37536085 MS.gene84077:CDS
ACCAGAGATGATCCTTTGGA+TGG 0.587544 1.4:+37536047 None:intergenic
TGTAAGAAACATCGTGACAA+GGG 0.589365 1.4:+37536278 None:intergenic
AGGAAAGTTGTTCAATGCAA+CGG 0.592106 1.4:+37530099 None:intergenic
GAAAGGCGCTCGTGACTTGG+GGG 0.593148 1.4:+37531040 None:intergenic
GGGTAATTACCGTTAGAAGA+GGG 0.594020 1.4:+37536590 None:intergenic
CTAATATACAACCTAGCCCA+AGG 0.600678 1.4:+37529817 None:intergenic
TATATTAGAAGATATCATGC+TGG 0.602589 1.4:-37529802 MS.gene84077:CDS
CATCTCTGGTGGTTTCCTGG+TGG 0.603360 1.4:-37536034 MS.gene84077:CDS
GCTTATAAATGTCTGTGATG+TGG 0.604531 1.4:-37531146 MS.gene84077:CDS
ATCATTTCAAATGACCCTGA+AGG 0.604755 1.4:+37533441 None:intergenic
ATATACTCGTTAAACTTCAA+GGG 0.610553 1.4:+37536301 None:intergenic
ATGTAAGAAACATCGTGACA+AGG 0.611925 1.4:+37536277 None:intergenic
GTTGTTGAATGAAGAAGGAG+AGG 0.612306 1.4:+37536619 None:intergenic
ATTCAATTTGCATTAGAGCG+AGG 0.614834 1.4:-37533510 MS.gene84077:CDS
GCATTCTGCAGAAGGCAGGA+GGG 0.614996 1.4:+37531178 None:intergenic
GACATAGTTCGAATATCCAA+GGG 0.618179 1.4:+37533564 None:intergenic
AACAACACTCCCTCTTCTAA+CGG 0.620325 1.4:-37536599 MS.gene84077:intron
TGGAGATGGATCGTCTTGCA+CGG 0.620920 1.4:-37529782 MS.gene84077:CDS
TTGGAGACTGATGAATGTCA+AGG 0.624402 1.4:-37530173 MS.gene84077:CDS
CCGGTCATAAATTCCAGACA+AGG 0.626956 1.4:+37530036 None:intergenic
TTGAAATGAGTACCACCACC+AGG 0.637461 1.4:+37536019 None:intergenic
ATACTGGGAAAAGAGGTAGT+TGG 0.639788 1.4:+37529845 None:intergenic
CCCAGTGTCAGGAATTACCA+AGG 0.646751 1.4:+37536336 None:intergenic
TGAACTCGAATAACAATGGG+AGG 0.652659 1.4:-37536782 None:intergenic
ATCCAAAGGATCATCTCTGG+TGG 0.653009 1.4:-37536045 MS.gene84077:CDS
ACTTGACTACAGCAATGTGT+TGG 0.655453 1.4:-37531255 MS.gene84077:CDS
CATCTTGCTGATGTCAAAGG+AGG 0.661435 1.4:-37536082 MS.gene84077:CDS
TTGTCTGGAATTTATGACCG+GGG 0.671528 1.4:-37530034 MS.gene84077:CDS
AATGTGACTCTACATCCCAA+AGG 0.673432 1.4:+37529399 None:intergenic
ACAAGTCAGAAGTTCTAATG+CGG 0.677007 1.4:-37531077 MS.gene84077:CDS
GAAAAGTTCCAATCAAACCC+CGG 0.678902 1.4:+37530017 None:intergenic
CTTGGTAATTCCTGACACTG+GGG 0.680113 1.4:-37536335 MS.gene84077:CDS
CCAGTGTCAGGAATTACCAA+GGG 0.688330 1.4:+37536337 None:intergenic
TTAAGTGGGATATTCCACGA+AGG 0.728759 1.4:+37531109 None:intergenic
TGTAAAAGATCATATGTACG+AGG 0.751315 1.4:+37529870 None:intergenic
GAGCATCTGAGTACATACAG+AGG 0.756387 1.4:-37535991 MS.gene84077:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr1.4 gene 37529328 37536789 37529328 ID=MS.gene84077
chr1.4 mRNA 37529328 37536789 37529328 ID=MS.gene84077.t1;Parent=MS.gene84077
chr1.4 exon 37536600 37536789 37536600 ID=MS.gene84077.t1.exon1;Parent=MS.gene84077.t1
chr1.4 CDS 37536600 37536789 37536600 ID=cds.MS.gene84077.t1;Parent=MS.gene84077.t1
chr1.4 exon 37535992 37536406 37535992 ID=MS.gene84077.t1.exon2;Parent=MS.gene84077.t1
chr1.4 CDS 37535992 37536406 37535992 ID=cds.MS.gene84077.t1;Parent=MS.gene84077.t1
chr1.4 exon 37533403 37533692 37533403 ID=MS.gene84077.t1.exon3;Parent=MS.gene84077.t1
chr1.4 CDS 37533403 37533692 37533403 ID=cds.MS.gene84077.t1;Parent=MS.gene84077.t1
chr1.4 exon 37531011 37531397 37531011 ID=MS.gene84077.t1.exon4;Parent=MS.gene84077.t1
chr1.4 CDS 37531011 37531397 37531011 ID=cds.MS.gene84077.t1;Parent=MS.gene84077.t1
chr1.4 exon 37530010 37530256 37530010 ID=MS.gene84077.t1.exon5;Parent=MS.gene84077.t1
chr1.4 CDS 37530010 37530256 37530010 ID=cds.MS.gene84077.t1;Parent=MS.gene84077.t1
chr1.4 exon 37529776 37529914 37529776 ID=MS.gene84077.t1.exon6;Parent=MS.gene84077.t1
chr1.4 CDS 37529776 37529914 37529776 ID=cds.MS.gene84077.t1;Parent=MS.gene84077.t1
chr1.4 exon 37529328 37529486 37529328 ID=MS.gene84077.t1.exon7;Parent=MS.gene84077.t1
chr1.4 CDS 37529328 37529486 37529328 ID=cds.MS.gene84077.t1;Parent=MS.gene84077.t1
Gene Sequence

>MS.gene84077

ATGGGAGGTTTTGCTACTATTGGTTCTGCCTTTGATTCTAAATCTGGCCAAATCATAATGGCTTCTTTACTTCTCATGATTGTTTCCTTTTATGTCGGTACTCTTTTTGGTAACAATACTCCTCTTTACATCTCTCACCTTCCTTCTTCCTCTCCTTCTTCATTCAACAACAACACTCCCTCTTCTAACGGTAATTACCCAATTTCATTTAATATCTATGAAGCACCGACACGACACCCAGACATATAATTACACCGAGTTACGTGATTTTCTCAAATTATTAGCGGTGTTAGTGTGTCAATGTTTGTGTCGTGTCTGGTGTCCGTGGTGTCCGTCCTTCAAATATGCTACTTTCTGACTCACTCATATGCAAATTTTTACAGGAACTTCACGATTTAGTAACAAAGTTGCTCTCACTTACCGCAAAATTCCCTTGGTAATTCCTGACACTGGGGTAGATATATGTCCCTTGAAGTTTAACGAGTATATCCCTTGTCACGATGTTTCTTACATTGCAACATTGATGTCTCATCTTGATGTTTCTAGAAAACAAGAGCTGGAGAGACATTGTCCTCCACTCGAAAACCGTTTGTTTTGTTTGGTTCCGCCGCCTAAGGATTATAAAATACCTATCAGATGGCCTCTTAGTAGGGATTATGTTTGGAGGAGTAATGTGAATCATACACATCTTGCTGATGTCAAAGGAGGCCAAAATTGGGTCCATCCAAAGGATCATCTCTGGTGGTTTCCTGGTGGTGGTACTCATTTCAAGAATGGAGCATCTGAGTACATACAGAGGTACCTATCTATACTTCTTTGGATGATTGATTACAACTTCATTTTTTTTTGCGTTGGATGTGTTGTAAGACTAGACATGATATGATTTGCAATGACAACATTAGAAAGAGAGAGAGTTCGGATAACACCTATAGTAGAAAAGAGAGTGGAAACCAGGCTTAAGTGGTTTGAGCATGTATAGAGAAAACTTGTAGATTTTGTAGTAAGGAGGGTAGTTAGGTCACTAGAGGCAGAGGTAGACCTAGAAAAACTTTAAGAGACATTGTTAGGAAAGATTTAGAGATTAATGAGTTGGATTCAAATATGGTTTATGATGGAACATTATTGCGTAATTTTATCCATGTAGCCGACCCCACTTAGTGGGATAAGGCTTGATTGTTTGCTGCATGAATTCTAGCGCGAGACAAATATTTTGAAATCAGATTGAGTGTGGCTCAGTGTTGTCAAACATGGTGATACTATGGCGGAGTGCGGTGATGGAATTTTTTACAACCACCATGGCCAATTTGTGAAAGAAGGCACCACCATGGCATTTTGTGACATGTGAACATATGATCTATTTCAATTTGCGAAATATTGAAGTTAATCCCATTTTAATACTGTTAAGTGTGGCACCCAAACATCTACCAGGAATTTCAAGTTTGAAGTAGATTGTGCTATAAGTTAAGATTGAGATAACTATGTGAGAGAGCAGGTAAAGATGAGTAGAGTAATGCCAGATGTAAATTTTTATCTCCTTTTGATAGTAATATATTTATACCCTGTGATTTGGGTCTACTGAGAATTCCTTATCTCGAGGGAGGTTCTTCTGCAAATAATTTGTGCATTTGTTTATTGAGCTTAGATTTTCTTTTGCAATTAAAAGGTATGCTTTATATCTTTAGAAAACTAAATAATCTTTTTACCCTGAATCCTGATCTTATTGAATTATTTGGAAGTTATTCTTAAGATAATATGTGACAAATTGACTGGTGTTGAATGGAAAATGACGTGACTGTTTGATTTTTAATTGTCTTTCAAAGGTGTAATCATATGGTCACCAAGCAAACTGCAACTGCGACTCACCTGTTATTTTGTTTAAAACATTAGATGGGAGAAAAGACAATGAAAGGAGAAATATTGTTTGAAATTGAAATTAAGCGAAATTTTTGGTCTTCAATTTCTGTTCACCAAATAATTAAAAGTTTCAAACTTTAAAGCTTTGAGAGTTAGGAGGGGATGGCTTTGGAGGAAAGCTAGAAAGGGAGGGTAAACACCCGTATATGGGGGAACTAAAATAAAAACGCGAGGAGGACTTTGCCGGGTGTAAAAGAAACACCGAACTCTTTCCTTCCCCTCTTTCTAAAAAGTCTAAAACAAGGGAATAACTTAAGTTCTCTTTCCTCTTCTCTCCTCTCACCTCCAGGAACCCATCCCAAACCAAGCCTTAAGATATATATATTATTTTTCTATCTTCGTTTTCTCAATCTAAACCTAGTGCCAGCGCTCCCTGAGCCTTGAGGCCTGCCCTGACCCTGGTCAGTATCTGGGACCCACTAACATAAACTTCTCTCGTGTGAAATCCACAAAAAAAAAAAAAAACCGTTAAGAAGTTAAAGTAACTATGTGAAATCCAGAAGATAATCAGTCACGGCTGACCACAGAAACTTCTCTCCTTATGTTTCTGTATTCTGAGACATATGTTTCGAGTACGATTTCCAATAAAAGAAAGTGTTATAAAACAAATTATGTTACAACGTTGTAGTAAAGAGGTAGCGCACAGTAGACTAAAATTCTTGGAGTCAATGGAACCACAAGGGTGTGTTTATTTTGAAAATGGTGCCTCAGGAGTTAACTTAATGAAGGAAAGGTTGTGGTTGACACATTGGAATGAATCCCTTTTCTCTCTGGCTTACTTTTGGACTTATGGCCTTGATACAAAAATCATAGTAAAAAATTGAATGTTGTTTGTAGATACTGCCTATTTCAGGCGATTGCTTTGTTGCAATTTGTAGTTAGTTTGTGCTATTGAAACAGAGTGAAACCAGATGCAGCTAACTGATATTTTTAAGGTTACATACACCGGTGAGAGGGAGAGAGGGGAGGGGAAGCTAATCTTGAGTCTTGACATTTTAATCATACATAAATGGTCAATATCAGTTTCAACTTTACTCGACAGTTTTAAGTAAAATCAATTTCTACCTATCTTGCAATTATTTTGCTTGGTGCTATGAACTAACTTGAGCATGGAATGTTTGTATGTTGTGCGTGAGTAGTGCAAGATACAACTGTTAGTCTTTTGATGGCTTTGCAAATGCAGGATAGGAAATATGATTACAAATGAGACGGGTGATTTACGTTCTGCGGGCATAGTTCAAGTTCTGGATCTTGGCTGTGGTGTGGCCAGCTTTTCAGCGTATCTTCTTCCCTTGGATATTCGAACTATGTCTTTTGCTCCCAAGGATGGTCATGAAAACCAGATTCAATTTGCATTAGAGCGAGGAATTGGTGCAATGATCTCTGCCATGTCCACAAAACAACTGCCATTTCCTTCAGGGTCATTTGAAATGATTCACTGTTCAAGATGCCGTATAGACTTTCATGAAAATGGTAATTGGCTTTCCTTATGATGTTTGTATATATTGGCTTTCCTTTCGAGGGGAGGGAAATTGAGTGGAGAGAAACTGAGAGGAAGTTTTGTTTATTTTTGTTTCATTGCAAAATAACACAAGAAAGGAAAAATTGATGTGGGACACACCTCTCGCCAAGTGGAGAGGTAACACCAATTTCAAGTAGACATTCACTCTTTCCAGCTATACTAATCTAATTATCTATCCTCTGTTGTGGGGTTCACTTGATTCTTATTTTGTCAAGGGTAAATATGACATTATATCAATTATTCATTTTCTTAATTTTCATTTTAATTATTGTCCAACCAAACAATTATAGAGAAAATTGAACTTTTCTCTGCACCCAAACGAAGCTTTGGTTGACAATAAACCAAGATAGAATGTGAACTTATTCGTAGAGTAAAATGGTATTTTGTCTAGTTTCGAAATGTTTTTAGCTCTCATTTGTTTATGATCTTTAATTCCTTTGGCACACTTCTTGAACGCAGTGGGTGATTTCCTCGACCCTTGCATTGCCGTAACTAGGTTCCTGCTACTTCAACACAGGGTTCTGCATATTCTTAAGCTTGAAATTCTCTCGAAATGATTGCTATATTTTTCCATTTCTTTCCCGGTGAGAGTTCTGGCAGATCTAACCAATTTATTAGGGACCAAGAATTAGGATGGAAATAAAGAATCTTAATATTAAAAAATTAAAAGAATATTAATAATATAATTCTGCCCATTGGTTTCTCCTTTACAAAAGATCTCTCCTTTTCTCTCTCATATCAAATGCACAGTATTCTATTTTCTTACTGTATTTCTCCTATTTATATTTGATACATCTTATCTAATTGACCAACTACTGACTAATCCTAATTACTACCCTTTATCTTTATCCTAACAGCTTGGTGTTAAACGTGTTTGATTCAGTCCTTTTATTTGAAAATCTTATTTATTGTTAATACATGCCTGATATAGCTAAAAGAAGTACTTTTGTGTTTACAAATTTTGATATTTTCTAAAACCAAAATGTTGGACAGCTTTCCATTTATGTATCACTGCAGTGCTCCAAAGTTGTTTAATTAGTTTGTGTTTTACCATAGTGAAATTTACTAATAACATTGATCTCAAGCCAACCAATGCATTGCAACACGTTAGAAGTATGTTTTGTCTCTTCTTAAGATTGACTTCCATTACCAATTTTGAAATCTGTCCCTGACCATTTCTTTTTTGCTACCGTTCTACCGCAAAGCATTCATTTATCTGACGAGCGATGAGTGACATTTTAACATATCTCTTTACTTTTGTTTGTGCAAAATATCATTTTATGCTATTGTTCCCTTGTTCAAGAAGTATATTTTTGTTATACGATACTTTAAAGAAGTTGTGTATTTTTAAGGATAAAAGGAAATACCCTCCCTCAACTATAGGTAATTTTACAAAACATGTGTTGGAATCTTATTAAACTAGCTCCCTAAAAACATGTTTGTAAAGTTAACTTTTATGTATGTATTGAGTTGAAGTAGCAGAGTTGTTAGATTTTGAACCATAATATTTTTTTTTTAATTTATTTTTTTAATTTTTTTAATTTTTAGAATTGAGCTTTGTTGAAGTTATTTCTTGCATTTTGTCTAGTTTTTAATAATTCATCTGTAAGGATATATCATCAAGAGAAAATAAAACAGAAAACAACAAAACAAGAAGTACTGGCATCATTATTCTCATTGTAGTTTTGAATTGTATAGCTTAGAGGTTTTCTTTTTCCCTTATTAGCTATCATCATCATCATCATCATCATTTTTGTTTTTATTGAATGACCTGGTACAATATGTTCCAAAGGCTGCTATAAACACTGATGGGTGAACCCTTGCCCAATTAGACCCATTTATTATTTCTGTTATAGATTCAATCTGACTATCTTGTAGAATAGATGAAATTTGTATATGTCAAAGCTATTATTGTTGGTGGGGTTGTTGGTTGCTAATTTATTTTGTTATACGCAGATGGGATTTTGCTGAAGGAGTTGGATCGCCTTCTTCGCTCAAATGGTTATTTTGTTTATTCAGCACCACCTGCTTACAGAAAAGATAAAGATTTTCCTGTCATTTGGGATAAGTTGGTGAACTTGACTACAGCAATGTGTTGGAGACTCATTGCTCGTAAAGTTCAGACTGCAATATGGATTAAAGAAAATAATCAGCCCTCCTGCCTTCTGCAGAATGCAGAACAAAAGCTTATAAATGTCTGTGATGTGGATGATGAGTCTAAACCTTCGTGGAATATCCCACTTAAGAACTGTATACAAGTCAGAAGTTCTAATGCGGAATCTTACAAGCTACCCCCAAGTCACGAGCGCCTTTCAGTGTTTTCTGAAAAGCTGAACAAGATAGGTCTGCCCATTCTCTTTTGATTTACTGTGCAAGAAGTTAATGTCCATATTTTACATTTTTCTTATTCTTAAATTATTCATACCAATCTTGATGTGCATGCTTACACCGTAGATATTTTGTATTCACATAATATAATTTTATTGCAAATCTCAGATAGCATCATATATCATGCATGCTCTCAGAAATTTCATGAACCGTGATTCCAATGTTTATGCTATTGATGCATATGCTTTATTCTCATCATAATGATTTTATATGGGCTATAGACGATATTTATCCCAAGGATTTAATCAAATGCACAATCTTCAGTCGTGCAGATATTGATGCTATTTTAATGATAATTTTTTTCAATTGTCAAAAACTTCAAACAAGGTCGGAACTCGAAACGTTTCTGACTTGCATAAATTATAATAAAAGGATAAAAGAAAAATACTTTTTTTTTTCTTGTTACTTTTCTGAATAAATTTGGGATATTATCATACAGGTATATAGTTGAACACCTCCCATAATTGCCTATGATATATGTGTTATCTTTTTAGTTCATTTACATACTGACATCAATATAGAAAGTCGCTATCTTGTTTGTTCCCTGTTTTTTAGTTGAGATTGGAAAGCATCAATACATTTAAATCTAAAGGACAATATTGAACAGCTGTTCAACTGGAAATTATAAGATAAAATAAATATGAATGTGGTGCACACAGCAGTCCCTTTCAGTCTAATGTGATGACCCTTCTAACCTTTTAAACAACAGGTATAAATCGAGATGAATTTACGTCAGATTCTATCTTCTGGCAAGATCAGACTCGTCATTATTGGAGACTGATGAATGTCAAGGAAACAGAAATACGCAACGTGATGGATATGAATGCCTTTTATGGTGGATTTGCCGTTGCATTGAACAACTTTCCTGTTTGGGTAATGAATGTAGTTCCTGCAAGCATGAAGAACACCTTGTCTGGAATTTATGACCGGGGTTTGATTGGAACTTTTCATGATTGGTAATATTTTGCAACTAATATCATAAATAGTAAATACAATGTTAAAATGTGTATTATTAGTTGACTGCAGCTAATGTAGATATCTTTTACATCAGGTGTGAGCCATTTTCAAGTTATCCTCGTACATATGATCTTTTACATGCCAACTACCTCTTTTCCCAGTATACAAACCTTGGGCTAGGTTGTATATTAGAAGATATCATGCTGGAGATGGATCGTCTTGCACGGCCACTGGTAATTAATTACTTTTTTAAATGTTACCTTCATGGTTCTCTGCATGTATTATTGAAGTCGAAGTTCATGTGACTGAAATAAAATAAGAATTTTGGTAAATTCTGATATACCAAAATACATCGGTTATTCAATGAATCTAAAAAGTGAATTGACTCTTATAATAAGGAACGGATGTAGTACAAAGTTTTGTCAAATAGCGGCTATAGCTACACTATAGCAGTGCAACATAGTGTTTATTTGTCTTCCTGTGGATAAAGTTGATAACTTAGAGCTCACTTATTATCTGCAGGGATTTATTATCATTAGGGATGAAGAACATATTATATCAAGAATCAACGATTTAGCCCCAAAATTCCTTTGGGATGTAGAGTCACATTTGCTAGAGAACAAAGAAAAGAAGATGGAAACTGTTTTAATTTGTAGAAAAAAGTTTTGGGCAATCCTCTAA
Protein sequence

>MS.gene84077.t1

MGGFATIGSAFDSKSGQIIMASLLLMIVSFYVGTLFGNNTPLYISHLPSSSPSSFNNNTPSSNGTSRFSNKVALTYRKIPLVIPDTGVDICPLKFNEYIPCHDVSYIATLMSHLDVSRKQELERHCPPLENRLFCLVPPPKDYKIPIRWPLSRDYVWRSNVNHTHLADVKGGQNWVHPKDHLWWFPGGGTHFKNGASEYIQRIGNMITNETGDLRSAGIVQVLDLGCGVASFSAYLLPLDIRTMSFAPKDGHENQIQFALERGIGAMISAMSTKQLPFPSGSFEMIHCSRCRIDFHENDGILLKELDRLLRSNGYFVYSAPPAYRKDKDFPVIWDKLVNLTTAMCWRLIARKVQTAIWIKENNQPSCLLQNAEQKLINVCDVDDESKPSWNIPLKNCIQVRSSNAESYKLPPSHERLSVFSEKLNKIGINRDEFTSDSIFWQDQTRHYWRLMNVKETEIRNVMDMNAFYGGFAVALNNFPVWVMNVVPASMKNTLSGIYDRGLIGTFHDWCEPFSSYPRTYDLLHANYLFSQYTNLGLGCILEDIMLEMDRLARPLGFIIIRDEEHIISRINDLAPKFLWDVESHLLENKEKKMETVLICRKKFWAIL