AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene028232

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene028232.t1 AT4G02620 87.302 126 16 0 1 126 1 126 4.46e-80 231

Find 29 sgRNAs with CRISPR-Local

Find 214 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACCATAGTTGGGTTTCTTT+TGG 0.252743 4.2:-16605009 MS.gene028232:CDS
CTCATGATTCAGTTCTTTCA+CGG 0.364488 4.2:-16603515 MS.gene028232:CDS
TTTCCACAGGATACCATAGT+TGG 0.389583 4.2:-16605020 MS.gene028232:intron
GAAGGGATTTCAAGAATTGC+AGG 0.394210 4.2:+16603561 None:intergenic
GGCCAACAGAGTTCCGATTC+CGG 0.408097 4.2:-16606291 MS.gene028232:CDS
ATTTCAAGAATTGCAGGAAC+TGG 0.410123 4.2:+16603567 None:intergenic
GATGAATTGTTGGCCGGAAT+CGG 0.420838 4.2:+16606278 None:intergenic
GAATCATGAGCAGGATCATA+AGG 0.423283 4.2:+16603528 None:intergenic
AGCCAAAAGAAACCCAACTA+TGG 0.431491 4.2:+16605007 None:intergenic
ACTTACTTGAATCCACAATA+AGG 0.438291 4.2:+16604946 None:intergenic
TGCAGGTTGCAAATATGATA+AGG 0.462958 4.2:-16603614 MS.gene028232:intron
GGCAATAAGTGATGAATTGT+TGG 0.470313 4.2:+16606268 None:intergenic
GAAGACAAATTACCTTATTG+TGG 0.485357 4.2:-16604958 MS.gene028232:CDS
ACTTACCTCGTCAGCAATCA+TGG 0.493837 4.2:+16606247 None:intergenic
TTCCACAGGATACCATAGTT+GGG 0.495606 4.2:-16605019 MS.gene028232:intron
TTCAATGCTGAATCAGTGGC+AGG 0.511114 4.2:-16603480 MS.gene028232:CDS
TCATGATTCAGTTCTTTCAC+GGG 0.514197 4.2:-16603514 MS.gene028232:CDS
GGCCGGAATCGGAACTCTGT+TGG 0.524456 4.2:+16606289 None:intergenic
AATCATGAGCAGGATCATAA+GGG 0.560484 4.2:+16603529 None:intergenic
GAAAGAACTGAATCATGAGC+AGG 0.572900 4.2:+16603519 None:intergenic
ATAAGTGATGAATTGTTGGC+CGG 0.586554 4.2:+16606272 None:intergenic
TATTGCCATGATTGCTGACG+AGG 0.587291 4.2:-16606252 MS.gene028232:intron
TCTCTTCAATGCTGAATCAG+TGG 0.629677 4.2:-16603484 MS.gene028232:CDS
AACGACTGTCAAACAAATCG+AGG 0.646091 4.2:-16604316 MS.gene028232:CDS
CAAAGAATTCTCAACACGGG+AGG 0.652854 4.2:-16604286 MS.gene028232:CDS
ATTCAAAGAATTCTCAACAC+GGG 0.675761 4.2:-16604289 MS.gene028232:CDS
CATTCAAAGAATTCTCAACA+CGG 0.678390 4.2:-16604290 MS.gene028232:CDS
CATGAGCAGGATCATAAGGG+TGG 0.700765 4.2:+16603532 None:intergenic
TGGGAAACGTTGACATACGT+CGG 0.705364 4.2:-16604980 MS.gene028232:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAAAAAAATCCAAAA+TGG + chr4.2:16604657-16604676 None:intergenic 10.0%
!!! GATTTTTTTTTTTTTGTAAT+TGG - chr4.2:16604662-16604681 MS.gene028232:intron 10.0%
!! ACTAGTTATTCAAATTAATA+GGG + chr4.2:16604569-16604588 None:intergenic 15.0%
!! TATGAAATGTATTAAAGATA+AGG + chr4.2:16605113-16605132 None:intergenic 15.0%
!! TTAATAAAAAAAGAAATCAG+TGG + chr4.2:16603956-16603975 None:intergenic 15.0%
!! TTACTTACTATTATTTATGA+GGG - chr4.2:16604851-16604870 MS.gene028232:intron 15.0%
!! TTTACTTACTATTATTTATG+AGG - chr4.2:16604850-16604869 MS.gene028232:intron 15.0%
!! AAAAAAATCCAAAATGGAAT+TGG + chr4.2:16604651-16604670 None:intergenic 20.0%
!! AAAAAATCCAAAATGGAATT+GGG + chr4.2:16604650-16604669 None:intergenic 20.0%
!! AAATACCAGCTTATATAATA+TGG + chr4.2:16604184-16604203 None:intergenic 20.0%
!! AATTTGTAAGTCATATTTCA+TGG - chr4.2:16605521-16605540 MS.gene028232:intron 20.0%
!! ATGAAATATGACTTACAAAT+TGG + chr4.2:16605522-16605541 None:intergenic 20.0%
!! ATTAACAGGAAAAAATTGTA+TGG + chr4.2:16604893-16604912 None:intergenic 20.0%
!! CACTAGTTATTCAAATTAAT+AGG + chr4.2:16604570-16604589 None:intergenic 20.0%
!! CTAGTTATTCAAATTAATAG+GGG + chr4.2:16604568-16604587 None:intergenic 20.0%
!! TAACAGGATTTAAATGAAAT+AGG - chr4.2:16604009-16604028 MS.gene028232:intron 20.0%
!! TTAACAGGAAAAAATTGTAT+GGG + chr4.2:16604892-16604911 None:intergenic 20.0%
!!! AAAATGTTGACTAGTTTATA+TGG - chr4.2:16605930-16605949 MS.gene028232:intron 20.0%
!!! AAATTTAATTTCTTTGGATG+AGG + chr4.2:16604050-16604069 None:intergenic 20.0%
!!! AACACATGTACTATTTTTAT+TGG + chr4.2:16604981-16605000 None:intergenic 20.0%
!!! AATTCAATTTTATTTTCCAC+AGG - chr4.2:16604727-16604746 MS.gene028232:intron 20.0%
!!! AGAGACAAATTTAATTTCTT+TGG + chr4.2:16604056-16604075 None:intergenic 20.0%
!!! ATAAGAAAGTATATACAACA+TGG + chr4.2:16603545-16603564 None:intergenic 20.0%
!!! TAAGAAAGTATATACAACAT+GGG + chr4.2:16603544-16603563 None:intergenic 20.0%
!!! TGTTTTATTTCCTTATTTCA+GGG + chr4.2:16604336-16604355 None:intergenic 20.0%
!!! TTAAAGATCTATTTTGCTAT+TGG - chr4.2:16603693-16603712 MS.gene028232:intron 20.0%
!!! TTCAAAACAAATTGATTTGA+CGG - chr4.2:16605384-16605403 MS.gene028232:intron 20.0%
!!! TTTTTGCTAGAATCTATTTA+AGG + chr4.2:16603988-16604007 None:intergenic 20.0%
! AAAATCAGATATAGTTCAGT+CGG - chr4.2:16604522-16604541 MS.gene028232:intron 25.0%
! AAATGAAATAGGAAATTGTG+AGG - chr4.2:16604020-16604039 MS.gene028232:intron 25.0%
! AACATGTGTTAAACTATGAT+AGG + chr4.2:16605682-16605701 None:intergenic 25.0%
! AATCAGAAAATTAGGTGAAA+AGG + chr4.2:16604947-16604966 None:intergenic 25.0%
! AATCAGATGATATGATAACT+AGG - chr4.2:16603593-16603612 MS.gene028232:CDS 25.0%
! AATGAATAATGCTAGATAAC+TGG - chr4.2:16605792-16605811 MS.gene028232:intron 25.0%
! AGATTCTAGCAAAAATTAAC+AGG - chr4.2:16603993-16604012 MS.gene028232:intron 25.0%
! ATACTCCTATTTCATTAACA+AGG + chr4.2:16604225-16604244 None:intergenic 25.0%
! CATTTCTACACATTCTATAT+AGG + chr4.2:16605074-16605093 None:intergenic 25.0%
! CTCTGATAAAATTGAAGAAA+GGG - chr4.2:16604074-16604093 MS.gene028232:intron 25.0%
! GTGAGATTCAATCATAAAAT+TGG - chr4.2:16605849-16605868 MS.gene028232:intron 25.0%
! TAAATAACCCAAGATTGTAA+AGG + chr4.2:16606104-16606123 None:intergenic 25.0%
! TAACAGGAAAAAATTGTATG+GGG + chr4.2:16604891-16604910 None:intergenic 25.0%
! TACTTCCATATTATATAAGC+TGG - chr4.2:16604176-16604195 MS.gene028232:intron 25.0%
! TAGCCAATTACTAGTATAAA+CGG - chr4.2:16604308-16604327 MS.gene028232:CDS 25.0%
! TCAGTTATTAGTTGGATATT+TGG - chr4.2:16605975-16605994 MS.gene028232:intron 25.0%
! TCTCTGATAAAATTGAAGAA+AGG - chr4.2:16604073-16604092 MS.gene028232:intron 25.0%
! TGTATTAAAGATAAGGTTTG+TGG + chr4.2:16605106-16605125 None:intergenic 25.0%
! TTTATGCAAAGGTTATTAGT+AGG - chr4.2:16605189-16605208 MS.gene028232:intron 25.0%
!! AAACCGTAGAATACTTATTT+TGG - chr4.2:16605152-16605171 MS.gene028232:intron 25.0%
!! AGGATAAGTAAAAAGTCTTT+TGG + chr4.2:16605657-16605676 None:intergenic 25.0%
!! ATTTTATGATTGAATCTCAC+TGG + chr4.2:16605849-16605868 None:intergenic 25.0%
!! TAGATTTGACTTAGAGTAAT+TGG + chr4.2:16604290-16604309 None:intergenic 25.0%
!! TGAAATAGTTTTTAAATCCC+AGG + chr4.2:16606040-16606059 None:intergenic 25.0%
!! TGCAAATATGATAAGGTTTT+TGG - chr4.2:16606153-16606172 MS.gene028232:intron 25.0%
!!! AACCGTAGAATACTTATTTT+GGG - chr4.2:16605153-16605172 MS.gene028232:intron 25.0%
!!! GTGTTTTATTTCCTTATTTC+AGG + chr4.2:16604337-16604356 None:intergenic 25.0%
!!! TTTTAATTATCTACTGCATC+AGG - chr4.2:16605748-16605767 MS.gene028232:intron 25.0%
AAAAAGAAATCAGTGGCTTT+AGG + chr4.2:16603949-16603968 None:intergenic 30.0%
AAAATCCATACAATGACAGT+TGG - chr4.2:16604373-16604392 MS.gene028232:intron 30.0%
AACAGGAAAAAATTGTATGG+GGG + chr4.2:16604890-16604909 None:intergenic 30.0%
AATGGAATTGGGAAATGATA+TGG + chr4.2:16604639-16604658 None:intergenic 30.0%
ACTTACTTGAATCCACAATA+AGG + chr4.2:16604817-16604836 None:intergenic 30.0%
AGAAAACCTTCTTGCTTATT+TGG - chr4.2:16606078-16606097 MS.gene028232:intron 30.0%
AGACTTATTGTTGTATATCG+TGG - chr4.2:16605017-16605036 MS.gene028232:intron 30.0%
AGTTTAACACATGTTCTGTT+TGG - chr4.2:16605687-16605706 MS.gene028232:intron 30.0%
ATGGAATTGGGAAATGATAT+GGG + chr4.2:16604638-16604657 None:intergenic 30.0%
ATTCAAAGAATTCTCAACAC+GGG - chr4.2:16605471-16605490 MS.gene028232:intron 30.0%
CATTCAAAGAATTCTCAACA+CGG - chr4.2:16605470-16605489 MS.gene028232:intron 30.0%
GAAGACAAATTACCTTATTG+TGG - chr4.2:16604802-16604821 MS.gene028232:intron 30.0%
GGTAATCAACACTATTGTTA+GGG - chr4.2:16605708-16605727 MS.gene028232:intron 30.0%
GTGTTAAACTATGATAGGTA+AGG + chr4.2:16605677-16605696 None:intergenic 30.0%
GTTCCGTTTATACTAGTAAT+TGG + chr4.2:16604314-16604333 None:intergenic 30.0%
TAAAAACTATTTCATGCACC+CGG - chr4.2:16606046-16606065 MS.gene028232:intron 30.0%
TAGAAAATAAGTGGATGGAT+AGG - chr4.2:16603855-16603874 MS.gene028232:intron 30.0%
TATAATTTCTTTGACTGTGC+AGG - chr4.2:16606129-16606148 MS.gene028232:intron 30.0%
TCTACCACAATCAGAAAATT+AGG + chr4.2:16604955-16604974 None:intergenic 30.0%
TCTTGAAAGGATCAAATTCA+GGG - chr4.2:16603830-16603849 MS.gene028232:intron 30.0%
TGACTTCTTAAGTCATCTTA+TGG + chr4.2:16603903-16603922 None:intergenic 30.0%
TGCCCAAAATAAGTATTCTA+CGG + chr4.2:16605158-16605177 None:intergenic 30.0%
TGGTAATCAACACTATTGTT+AGG - chr4.2:16605707-16605726 MS.gene028232:intron 30.0%
TTAATTGGTACTTGAACCTT+GGG - chr4.2:16605228-16605247 MS.gene028232:intron 30.0%
TTCAGGGTTTAGAAAATAAG+TGG - chr4.2:16603846-16603865 MS.gene028232:intron 30.0%
! AACTGTCATTGTATGGATTT+TGG + chr4.2:16604374-16604393 None:intergenic 30.0%
! AAGGTTTTCTAAATAGGAAC+CGG + chr4.2:16606068-16606087 None:intergenic 30.0%
! AAGTATTCTACGGTTTTTCT+GGG + chr4.2:16605148-16605167 None:intergenic 30.0%
! ACTATCATTGTACATTTTCC+TGG - chr4.2:16603760-16603779 MS.gene028232:intron 30.0%
! AGCAAGAAGGTTTTCTAAAT+AGG + chr4.2:16606074-16606093 None:intergenic 30.0%
! ATAGTTTTTAAATCCCAGGT+AGG + chr4.2:16606036-16606055 None:intergenic 30.0%
! ATCATTTCCCAATTCCATTT+TGG - chr4.2:16604640-16604659 MS.gene028232:intron 30.0%
! CATTAACCTCATGTAAGTTT+TGG + chr4.2:16605776-16605795 None:intergenic 30.0%
! GATTGCCTTGTTAATGAAAT+AGG - chr4.2:16604217-16604236 MS.gene028232:intron 30.0%
! TAAGTATTCTACGGTTTTTC+TGG + chr4.2:16605149-16605168 None:intergenic 30.0%
! TATTAGCTACGATGATTTTG+AGG + chr4.2:16603642-16603661 None:intergenic 30.0%
! TATTTGGCCTTTACAATCTT+GGG - chr4.2:16606094-16606113 MS.gene028232:intron 30.0%
! TTATTTGGCCTTTACAATCT+TGG - chr4.2:16606093-16606112 MS.gene028232:intron 30.0%
! TTCACCTAATTTTCTGATTG+TGG - chr4.2:16604948-16604967 MS.gene028232:intron 30.0%
!! AACAGCCTGAATCATTTTTA+TGG + chr4.2:16605547-16605566 None:intergenic 30.0%
!! TCAAGTTCTGAAAATATTCC+TGG - chr4.2:16605345-16605364 MS.gene028232:intron 30.0%
!! TGACTAGTTTATATGGTCAT+TGG - chr4.2:16605937-16605956 MS.gene028232:intron 30.0%
!!! AGAATACTTATTTTGGGCAT+GGG - chr4.2:16605159-16605178 MS.gene028232:intron 30.0%
!!! ATTGTATGGATTTTGGCTTT+GGG + chr4.2:16604367-16604386 None:intergenic 30.0%
!!! TAGAATACTTATTTTGGGCA+TGG - chr4.2:16605158-16605177 MS.gene028232:intron 30.0%
!!! TGGGCTATTTTTTTATGCAA+AGG - chr4.2:16605178-16605197 MS.gene028232:intron 30.0%
AAACTGAGCAAGAAATTACC+AGG + chr4.2:16605366-16605385 None:intergenic 35.0%
AATCATGAGCAGGATCATAA+GGG + chr4.2:16606234-16606253 None:intergenic 35.0%
ACCAATTAACCTCAGTTTCA+GGG + chr4.2:16605217-16605236 None:intergenic 35.0%
ACTAGGATGCATGATTGAAT+AGG - chr4.2:16603610-16603629 MS.gene028232:CDS 35.0%
AGAAAATTAGGTGAAAAGGC+AGG + chr4.2:16604943-16604962 None:intergenic 35.0%
ATAAACGGAACCCTGAAATA+AGG - chr4.2:16604323-16604342 MS.gene028232:intron 35.0%
ATAAGTGATGAATTGTTGGC+CGG + chr4.2:16603491-16603510 None:intergenic 35.0%
ATGTAATAAGCTGATGTCTC+AGG + chr4.2:16605634-16605653 None:intergenic 35.0%
ATTGTTAGGGAAAATCTGGA+GGG - chr4.2:16605721-16605740 MS.gene028232:intron 35.0%
ATTTCAAGAATTGCAGGAAC+TGG + chr4.2:16606196-16606215 None:intergenic 35.0%
CACTATTGTTAGGGAAAATC+TGG - chr4.2:16605717-16605736 MS.gene028232:intron 35.0%
CATGGCCATAAAAATGATTC+AGG - chr4.2:16605539-16605558 MS.gene028232:intron 35.0%
CTAGGATGCATGATTGAATA+GGG - chr4.2:16603611-16603630 MS.gene028232:CDS 35.0%
CTCATGATTCAGTTCTTTCA+CGG - chr4.2:16606245-16606264 MS.gene028232:intron 35.0%
CTCTTGAAAGGATCAAATTC+AGG - chr4.2:16603829-16603848 MS.gene028232:intron 35.0%
CTGTTGGATCAGTTATTAGT+TGG - chr4.2:16605967-16605986 MS.gene028232:intron 35.0%
GAAAATTAGGTGAAAAGGCA+GGG + chr4.2:16604942-16604961 None:intergenic 35.0%
GAAAGGGATTTAGAGATCAT+AGG - chr4.2:16604090-16604109 MS.gene028232:intron 35.0%
GGCAATAAGTGATGAATTGT+TGG + chr4.2:16603495-16603514 None:intergenic 35.0%
GGGTTTAATAGGTTAGGTTT+AGG - chr4.2:16603804-16603823 MS.gene028232:intron 35.0%
GGGTTTAGAAAATAAGTGGA+TGG - chr4.2:16603850-16603869 MS.gene028232:intron 35.0%
GTTAATTGGTACTTGAACCT+TGG - chr4.2:16605227-16605246 MS.gene028232:intron 35.0%
TAAAGGCCAAATAAGCAAGA+AGG + chr4.2:16606087-16606106 None:intergenic 35.0%
TACCAATTAACCTCAGTTTC+AGG + chr4.2:16605218-16605237 None:intergenic 35.0%
TATTGTTAGGGAAAATCTGG+AGG - chr4.2:16605720-16605739 MS.gene028232:intron 35.0%
TCATGATTCAGTTCTTTCAC+GGG - chr4.2:16606246-16606265 MS.gene028232:intron 35.0%
TGCAGGTTGCAAATATGATA+AGG - chr4.2:16606146-16606165 MS.gene028232:intron 35.0%
TTGTCGACATTTAAACCCAA+AGG + chr4.2:16605894-16605913 None:intergenic 35.0%
! ACTTTCAGTTTTCAGCCATA+GGG + chr4.2:16604466-16604485 None:intergenic 35.0%
! AGGTTTTCTAAATAGGAACC+GGG + chr4.2:16606067-16606086 None:intergenic 35.0%
! AGTATTCTACGGTTTTTCTG+GGG + chr4.2:16605147-16605166 None:intergenic 35.0%
! TACCATAGTTGGGTTTCTTT+TGG - chr4.2:16604751-16604770 MS.gene028232:intron 35.0%
! TACTTTCAGTTTTCAGCCAT+AGG + chr4.2:16604467-16604486 None:intergenic 35.0%
! TAGGTTTAGGTTCTCTTGAA+AGG - chr4.2:16603817-16603836 MS.gene028232:intron 35.0%
!! TTTTCTTAAGAAGTGTGCAG+TGG + chr4.2:16603737-16603756 None:intergenic 35.0%
!!! CATTGTATGGATTTTGGCTT+TGG + chr4.2:16604368-16604387 None:intergenic 35.0%
!!! TTGTATGGATTTTGGCTTTG+GGG + chr4.2:16604366-16604385 None:intergenic 35.0%
AACATGACGCACAGATTAAC+AGG + chr4.2:16604907-16604926 None:intergenic 40.0%
AACGACTGTCAAACAAATCG+AGG - chr4.2:16605444-16605463 MS.gene028232:intron 40.0%
ACACTCCAACTGTCATTGTA+TGG + chr4.2:16604381-16604400 None:intergenic 40.0%
ACCCTGAAACTGAGGTTAAT+TGG - chr4.2:16605213-16605232 MS.gene028232:intron 40.0%
ACCGCACTAAAATATCCCTA+TGG - chr4.2:16604448-16604467 MS.gene028232:intron 40.0%
ACTGCTTGGTACAAATCACA+GGG + chr4.2:16604253-16604272 None:intergenic 40.0%
AGCCAAAAGAAACCCAACTA+TGG + chr4.2:16604756-16604775 None:intergenic 40.0%
AGTAATCCAAGTACTACCCA+AGG + chr4.2:16605247-16605266 None:intergenic 40.0%
AGTAATTGGACCTGCAGTTT+CGG + chr4.2:16604276-16604295 None:intergenic 40.0%
CAATGGCTGATTGAGCTAAA+AGG + chr4.2:16606007-16606026 None:intergenic 40.0%
CAGGTTCCAAAACTTACATG+AGG - chr4.2:16605767-16605786 MS.gene028232:intron 40.0%
CGACATTTAAACCCAAAGGA+TGG + chr4.2:16605890-16605909 None:intergenic 40.0%
CGGCAATTGAACATTACAAC+TGG - chr4.2:16604542-16604561 MS.gene028232:intron 40.0%
CTGTTAGTTCCTACTTCCTA+CGG - chr4.2:16605608-16605627 MS.gene028232:intron 40.0%
GAAAGAACTGAATCATGAGC+AGG + chr4.2:16606244-16606263 None:intergenic 40.0%
GAAGGGATTTCAAGAATTGC+AGG + chr4.2:16606202-16606221 None:intergenic 40.0%
GAATCATGAGCAGGATCATA+AGG + chr4.2:16606235-16606254 None:intergenic 40.0%
GATTGCATCACACATAGTCA+AGG - chr4.2:16604143-16604162 MS.gene028232:intron 40.0%
TACAATGACAGTTGGAGTGT+TGG - chr4.2:16604381-16604400 MS.gene028232:intron 40.0%
TAGAAGGCTTGGGGTTTAAT+AGG - chr4.2:16603793-16603812 MS.gene028232:intron 40.0%
TAGGAAGTAGGAACTAACAG+TGG + chr4.2:16605608-16605627 None:intergenic 40.0%
TATCCCCATGCTTATTTACG+AGG + chr4.2:16605044-16605063 None:intergenic 40.0%
TCTCTTCAATGCTGAATCAG+TGG - chr4.2:16606276-16606295 MS.gene028232:CDS 40.0%
TCTTTCGATAGCCATCCTTT+GGG - chr4.2:16605876-16605895 MS.gene028232:intron 40.0%
TGGACCTCGTAAATAAGCAT+GGG - chr4.2:16605037-16605056 MS.gene028232:intron 40.0%
TTCCACAGGATACCATAGTT+GGG - chr4.2:16604741-16604760 MS.gene028232:intron 40.0%
TTCTTTCGATAGCCATCCTT+TGG - chr4.2:16605875-16605894 MS.gene028232:intron 40.0%
TTTCCACAGGATACCATAGT+TGG - chr4.2:16604740-16604759 MS.gene028232:intron 40.0%
! ATAGTTGGGTTTCTTTTGGC+TGG - chr4.2:16604755-16604774 MS.gene028232:intron 40.0%
! CATAAGGGTGGTCTTTTGAA+GGG + chr4.2:16606219-16606238 None:intergenic 40.0%
! CATTGTACATTTTCCTGGTG+TGG - chr4.2:16603765-16603784 MS.gene028232:intron 40.0%
! TCATAAGGGTGGTCTTTTGA+AGG + chr4.2:16606220-16606239 None:intergenic 40.0%
!!! GCCATAGGGATATTTTAGTG+CGG + chr4.2:16604452-16604471 None:intergenic 40.0%
!!! TGTATGGATTTTGGCTTTGG+GGG + chr4.2:16604365-16604384 None:intergenic 40.0%
AACCCAACTATGGTATCCTG+TGG + chr4.2:16604746-16604765 None:intergenic 45.0%
AAGTAGGAACTAACAGTGGC+AGG + chr4.2:16605604-16605623 None:intergenic 45.0%
ACTTACCTCGTCAGCAATCA+TGG + chr4.2:16603516-16603535 None:intergenic 45.0%
AGCCATTGTGATATCCTACC+TGG - chr4.2:16606019-16606038 MS.gene028232:intron 45.0%
ATCTGTGCGTCATGTTCATG+TGG - chr4.2:16604911-16604930 MS.gene028232:intron 45.0%
ATGGATAGGTTCTCGAGGAA+GGG - chr4.2:16603869-16603888 MS.gene028232:intron 45.0%
CAAAGAATTCTCAACACGGG+AGG - chr4.2:16605474-16605493 MS.gene028232:intron 45.0%
CTTGAACCTTGGGTAGTACT+TGG - chr4.2:16605238-16605257 MS.gene028232:intron 45.0%
GACTGCTTGGTACAAATCAC+AGG + chr4.2:16604254-16604273 None:intergenic 45.0%
GATGAATTGTTGGCCGGAAT+CGG + chr4.2:16603485-16603504 None:intergenic 45.0%
GCCATTGTGATATCCTACCT+GGG - chr4.2:16606020-16606039 MS.gene028232:intron 45.0%
GGACCTCGTAAATAAGCATG+GGG - chr4.2:16605038-16605057 MS.gene028232:intron 45.0%
GGCTGATTGAGCTAAAAGGA+CGG + chr4.2:16606003-16606022 None:intergenic 45.0%
GGCTTGGGGTTTAATAGGTT+AGG - chr4.2:16603798-16603817 MS.gene028232:intron 45.0%
GTGGACCTCGTAAATAAGCA+TGG - chr4.2:16605036-16605055 MS.gene028232:intron 45.0%
TAGTAGGTACCCTGAAACTG+AGG - chr4.2:16605205-16605224 MS.gene028232:intron 45.0%
TATTGCCATGATTGCTGACG+AGG - chr4.2:16603508-16603527 MS.gene028232:CDS 45.0%
TCCCAGGTAGGATATCACAA+TGG + chr4.2:16606024-16606043 None:intergenic 45.0%
TCTCACTGGTACTGTCACAT+TGG + chr4.2:16605835-16605854 None:intergenic 45.0%
TGCATCACACATAGTCAAGG+AGG - chr4.2:16604146-16604165 MS.gene028232:intron 45.0%
TGGGAAACGTTGACATACGT+CGG - chr4.2:16604780-16604799 MS.gene028232:intron 45.0%
TTCAATGCTGAATCAGTGGC+AGG - chr4.2:16606280-16606299 MS.gene028232:CDS 45.0%
! TGTGGAGTACTAGAAGGCTT+GGG - chr4.2:16603783-16603802 MS.gene028232:intron 45.0%
!! GGTCATTGGTTTCTCACTGT+TGG - chr4.2:16605951-16605970 MS.gene028232:intron 45.0%
!!! TATTTTAGTGCGGTGCTGCT+AGG + chr4.2:16604442-16604461 None:intergenic 45.0%
AGTGGATGGATAGGTTCTCG+AGG - chr4.2:16603864-16603883 MS.gene028232:intron 50.0%
CATGAGCAGGATCATAAGGG+TGG + chr4.2:16606231-16606250 None:intergenic 50.0%
GATGGATAGGTTCTCGAGGA+AGG - chr4.2:16603868-16603887 MS.gene028232:intron 50.0%
GTTCTCGAGGAAGGGACTTT+CGG - chr4.2:16603877-16603896 MS.gene028232:intron 50.0%
! GTGGAGTACTAGAAGGCTTG+GGG - chr4.2:16603784-16603803 MS.gene028232:intron 50.0%
! GTGTGGAGTACTAGAAGGCT+TGG - chr4.2:16603782-16603801 MS.gene028232:intron 50.0%
! TCCTGGTGTGGAGTACTAGA+AGG - chr4.2:16603777-16603796 MS.gene028232:intron 50.0%
!! GGGTTTCTTTTGGCTGGAGT+GGG - chr4.2:16604761-16604780 MS.gene028232:intron 50.0%
!! TGGGTTTCTTTTGGCTGGAG+TGG - chr4.2:16604760-16604779 MS.gene028232:intron 50.0%
AAGCTGATGTCTCAGGCCGT+AGG + chr4.2:16605627-16605646 None:intergenic 55.0%
ACCAAGCAGTCCGAAACTGC+AGG - chr4.2:16604263-16604282 MS.gene028232:CDS 55.0%
ACCTGCAGTTTCGGACTGCT+TGG + chr4.2:16604267-16604286 None:intergenic 55.0%
GCCTTCTAGTACTCCACACC+AGG + chr4.2:16603781-16603800 None:intergenic 55.0%
GGCCAACAGAGTTCCGATTC+CGG - chr4.2:16603469-16603488 MS.gene028232:CDS 55.0%
TGTCTCAGGCCGTAGGAAGT+AGG + chr4.2:16605620-16605639 None:intergenic 55.0%
GGCCGGAATCGGAACTCTGT+TGG + chr4.2:16603474-16603493 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 16603467 16606315 16603467 ID=MS.gene028232
chr4.2 mRNA 16603467 16606315 16603467 ID=MS.gene028232.t1;Parent=MS.gene028232
chr4.2 exon 16606253 16606315 16606253 ID=MS.gene028232.t1.exon1;Parent=MS.gene028232.t1
chr4.2 CDS 16606253 16606315 16606253 ID=cds.MS.gene028232.t1;Parent=MS.gene028232.t1
chr4.2 exon 16604952 16605033 16604952 ID=MS.gene028232.t1.exon2;Parent=MS.gene028232.t1
chr4.2 CDS 16604952 16605033 16604952 ID=cds.MS.gene028232.t1;Parent=MS.gene028232.t1
chr4.2 exon 16604257 16604339 16604257 ID=MS.gene028232.t1.exon3;Parent=MS.gene028232.t1
chr4.2 CDS 16604257 16604339 16604257 ID=cds.MS.gene028232.t1;Parent=MS.gene028232.t1
chr4.2 exon 16603467 16603631 16603467 ID=MS.gene028232.t1.exon4;Parent=MS.gene028232.t1
chr4.2 CDS 16603467 16603631 16603467 ID=cds.MS.gene028232.t1;Parent=MS.gene028232.t1
Gene Sequence

>MS.gene028232

ATGGCCAACAGAGTTCCGATTCCGGCCAACAATTCATCACTTATTGCCATGATTGCTGACGAGGTAAGTGCGTGCCCATGTTGTATATACTTTCTTATCAGTGATATGTTGTCTAAAAGAATGTCAAATCAGATGATATGATAACTAGGATGCATGATTGAATAGGGTTTAGCCTCAAAATCATCGTAGCTAATATAAGTTCGCTGCTAGAGAATTTTGTGATATTTTAAAGATCTATTTTGCTATTGGCGTACAACTCATTATGCACCACTGCACACTTCTTAAGAAAACAAACTATCATTGTACATTTTCCTGGTGTGGAGTACTAGAAGGCTTGGGGTTTAATAGGTTAGGTTTAGGTTCTCTTGAAAGGATCAAATTCAGGGTTTAGAAAATAAGTGGATGGATAGGTTCTCGAGGAAGGGACTTTCGGCCATAAGATGACTTAAGAAGTCAGATTGACGACATTTCAGATGCTGCCTAAAGCCACTGATTTCTTTTTTTATTAATTGAGTTTTCCTTAAATAGATTCTAGCAAAAATTAACAGGATTTAAATGAAATAGGAAATTGTGAGGTCGTCCTCATCCAAAGAAATTAAATTTGTCTCTCTGATAAAATTGAAGAAAGGGATTTAGAGATCATAGGAAAAGAAGAAAATCATCATACATTGAGTTTGATTGCATCACACATAGTCAAGGAGGAGCAATATACTTCCATATTATATAAGCTGGTATTTTATTGATGTTTTTGATTGCCTTGTTAATGAAATAGGAGTATAAGTACCCTGTGATTTGTACCAAGCAGTCCGAAACTGCAGGTCCAATTACTCTAAGTCAAATCTAGCCAATTACTAGTATAAACGGAACCCTGAAATAAGGAAATAAAACACAAACTCCCCCAAAGCCAAAATCCATACAATGACAGTTGGAGTGTTGGACATGAGTATATGACTAGATACTTAACTATACAGTCCTAGCAGCACCGCACTAAAATATCCCTATGGCTGAAAACTGAAAGTATAATAATCTTTTGACATTATAAGTTTTCTAATCTTAAAATCAGATATAGTTCAGTCGGCAATTGAACATTACAACTGGCCCCTATTAATTTGAATAACTAGTGAGATATTATATTCTTTGAGCTTCTCTTTTTTATATTCTAATTTTGCCCATATCATTTCCCAATTCCATTTTGGATTTTTTTTTTTTTGTAATTGGTATTCATTATGATTGAAATGATTAAAATGTTCTGAGATTTTGAATTCAATTTTATTTTCCACAGGATACCATAGTTGGGTTTCTTTTGGCTGGAGTGGGAAACGTTGACATACGTCGGAAGACAAATTACCTTATTGTGGATTCAAGTAAGTCACTAAACTATATTTTACTTACTATTATTTATGAGGGCTAGTTTCGAATTCCCCCATACAATTTTTTCCTGTTAATCTGTGCGTCATGTTCATGTGGAGACTCCCTGCCTTTTCACCTAATTTTCTGATTGTGGTAGATGTCCAATAAAAATAGTACATGTGTTCTTTTATGTTTTGTAAAGACTTATTGTTGTATATCGTGGACCTCGTAAATAAGCATGGGGATATGTCTCTCCTATATAGAATGTGTAGAAATGTTGTGATTTCCACAAACCTTATCTTTAATACATTTCATAGCTTAGACACTCCCCAGAAAAACCGTAGAATACTTATTTTGGGCATGGGCTATTTTTTTATGCAAAGGTTATTAGTAGGTACCCTGAAACTGAGGTTAATTGGTACTTGAACCTTGGGTAGTACTTGGATTACTCACTACAGATTGATAAGATAGCATCATTTTTTTTTTTTATTTTAAATATAGCATCATTTTTTCTCTCTGAATAGTGTGTCAAGTTCTGAAAATATTCCTGGTAATTTCTTGCTCAGTTTCAAAACAAATTGATTTGACGGAAATTTTTTGTTTTAACTCTTGATTTTGATCTTCAGAAACGACTGTCAAACAAATCGAGGATGCATTCAAAGAATTCTCAACACGGGAGGATATTGCAATTGTGCTGATTAGCCAATTTGTAAGTCATATTTCATGGCCATAAAAATGATTCAGGCTGTTTTGTTCTTGCTTCTTAGTTAAGTAAAACACTATACCTGCCACTGTTAGTTCCTACTTCCTACGGCCTGAGACATCAGCTTATTACATCCAAAAGACTTTTTACTTATCCTTACCTATCATAGTTTAACACATGTTCTGTTTGGTAATCAACACTATTGTTAGGGAAAATCTGGAGGGAATATTTTAATTATCTACTGCATCAGGTTCCAAAACTTACATGAGGTTAATGAATAATGCTAGATAACTGGAGATTTTATTTGACAGACCAATGTGACAGTACCAGTGAGATTCAATCATAAAATTGGCACTTCTTTCGATAGCCATCCTTTGGGTTTAAATGTCGACAAGTTATATTATTGCAAAAAAATGTTGACTAGTTTATATGGTCATTGGTTTCTCACTGTTGGATCAGTTATTAGTTGGATATTTGGATCCGTCCTTTTAGCTCAATCAGCCATTGTGATATCCTACCTGGGATTTAAAAACTATTTCATGCACCCGGTTCCTATTTAGAAAACCTTCTTGCTTATTTGGCCTTTACAATCTTGGGTTATTTATATCTTATAATTTCTTTGACTGTGCAGGTTGCAAATATGATAAGGTTTTTGGTTGATAGTTATAACAAGCCAGTTCCTGCAATTCTTGAAATCCCTTCAAAAGACCACCCTTATGATCCTGCTCATGATTCAGTTCTTTCACGGGTGAAGTATCTCTTCAATGCTGAATCAGTGGCAGGTGATAGACGTTGA
Protein sequence

>MS.gene028232.t1

MANRVPIPANNSSLIAMIADEDTIVGFLLAGVGNVDIRRKTNYLIVDSKTTVKQIEDAFKEFSTREDIAIVLISQFVANMIRFLVDSYNKPVPAILEIPSKDHPYDPAHDSVLSRVKYLFNAESVAGDRR