Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene028711.t1 | XP_002946472.1 | 98.6 | 73 | 1 | 0 | 1 | 73 | 1 | 73 | 7.40E-34 | 152.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene028711.t1 | Q9FGZ9 | 93.2 | 73 | 5 | 0 | 1 | 73 | 1 | 73 | 4.9e-34 | 144.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene028711.t1 | D8TJM7 | 98.6 | 73 | 1 | 0 | 1 | 73 | 1 | 73 | 5.4e-34 | 152.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene028711 | MS.gene045369 | PPI |
| MS.gene074354 | MS.gene028711 | PPI |
| MS.gene79884 | MS.gene028711 | PPI |
| MS.gene028711 | MS.gene31284 | PPI |
| MS.gene028709 | MS.gene028711 | PPI |
| MS.gene75680 | MS.gene028711 | PPI |
| MS.gene77522 | MS.gene028711 | PPI |
| MS.gene028711 | MS.gene058936 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene028711.t1 | MTR_3g084140 | 95.890 | 73 | 3 | 0 | 1 | 73 | 1 | 73 | 5.55e-47 | 144 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene028711.t1 | AT3G45180 | 93.151 | 73 | 5 | 0 | 1 | 73 | 1 | 73 | 4.11e-46 | 141 |
| MS.gene028711.t1 | AT3G45180 | 93.151 | 73 | 5 | 0 | 1 | 73 | 1 | 73 | 4.11e-46 | 141 |
| MS.gene028711.t1 | AT5G42300 | 93.151 | 73 | 5 | 0 | 1 | 73 | 1 | 73 | 1.30e-45 | 140 |
Find 12 sgRNAs with CRISPR-Local
Find 13 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACAAAATTCGGATTCAGAAA+TGG | 0.431017 | 2.1:-8816408 | MS.gene028711:CDS |
| GTACAAGACCTGACAAAATT+CGG | 0.445316 | 2.1:-8816420 | MS.gene028711:CDS |
| GAACGATCGGTTAGGGAAGA+AGG | 0.472486 | 2.1:-8816509 | MS.gene028711:CDS |
| AAACTCGTTGCTGCACAAAC+AGG | 0.476026 | 2.1:-8816442 | MS.gene028711:CDS |
| ATTAGTTATAGTAGAGTTCG+AGG | 0.477137 | 2.1:+8816326 | None:intergenic |
| TCGATCGAAGAAGATGATAG+AGG | 0.558716 | 2.1:-8816539 | None:intergenic |
| TACGAGATTCATGATGGCAT+GGG | 0.579214 | 2.1:-8816349 | MS.gene028711:CDS |
| TTACGAGATTCATGATGGCA+TGG | 0.596991 | 2.1:-8816350 | MS.gene028711:CDS |
| TCTGATTACGAGATTCATGA+TGG | 0.607663 | 2.1:-8816355 | MS.gene028711:CDS |
| GAGTTTCTTCAAGTCACCGA+TGG | 0.637090 | 2.1:+8816459 | None:intergenic |
| ATCGAAGAAGATGATAGAGG+TGG | 0.670468 | 2.1:-8816536 | None:intergenic |
| TGCAACGAAGATGATACCAT+CGG | 0.675479 | 2.1:-8816475 | MS.gene028711:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ACAAAATTCGGATTCAGAAA+TGG | - | chr2.1:8816445-8816464 | MS.gene028711:CDS | 30.0% | |
| GTACAAGACCTGACAAAATT+CGG | - | chr2.1:8816433-8816452 | MS.gene028711:CDS | 35.0% | |
| TCTGATTACGAGATTCATGA+TGG | - | chr2.1:8816498-8816517 | MS.gene028711:CDS | 35.0% | |
| ! | TTCTGAATCCGAATTTTGTC+AGG | + | chr2.1:8816444-8816463 | None:intergenic | 35.0% |
| TACGAGATTCATGATGGCAT+GGG | - | chr2.1:8816504-8816523 | MS.gene028711:CDS | 40.0% | |
| TGCAACGAAGATGATACCAT+CGG | - | chr2.1:8816378-8816397 | MS.gene028711:CDS | 40.0% | |
| TTACGAGATTCATGATGGCA+TGG | - | chr2.1:8816503-8816522 | MS.gene028711:CDS | 40.0% | |
| !!! | TAGAGGTGGTTTTGAACGAT+CGG | - | chr2.1:8816331-8816350 | MS.gene028711:CDS | 40.0% |
| !!! | TGGTTTTGAACGATCGGTTA+GGG | - | chr2.1:8816337-8816356 | MS.gene028711:CDS | 40.0% |
| AAACTCGTTGCTGCACAAAC+AGG | - | chr2.1:8816411-8816430 | MS.gene028711:CDS | 45.0% | |
| GAGTTTCTTCAAGTCACCGA+TGG | + | chr2.1:8816397-8816416 | None:intergenic | 45.0% | |
| !!! | GTGGTTTTGAACGATCGGTT+AGG | - | chr2.1:8816336-8816355 | MS.gene028711:CDS | 45.0% |
| GAACGATCGGTTAGGGAAGA+AGG | - | chr2.1:8816344-8816363 | MS.gene028711:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.1 | gene | 8816327 | 8816548 | 8816327 | ID=MS.gene028711 |
| chr2.1 | mRNA | 8816327 | 8816548 | 8816327 | ID=MS.gene028711.t1;Parent=MS.gene028711 |
| chr2.1 | exon | 8816327 | 8816548 | 8816327 | ID=MS.gene028711.t1.exon1;Parent=MS.gene028711.t1 |
| chr2.1 | CDS | 8816327 | 8816548 | 8816327 | ID=cds.MS.gene028711.t1;Parent=MS.gene028711.t1 |
| Gene Sequence |
| Protein sequence |
