Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31284.t1 | XP_002946472.1 | 98.6 | 73 | 1 | 0 | 1 | 73 | 1 | 73 | 7.40E-34 | 152.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31284.t1 | Q9FGZ9 | 93.2 | 73 | 5 | 0 | 1 | 73 | 1 | 73 | 4.9e-34 | 144.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31284.t1 | D8TJM7 | 98.6 | 73 | 1 | 0 | 1 | 73 | 1 | 73 | 5.4e-34 | 152.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene058936 | MS.gene31284 | 0.949059 | 2.73E-107 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene31284 | MS.gene045369 | PPI |
| MS.gene058936 | MS.gene31284 | PPI |
| MS.gene79884 | MS.gene31284 | PPI |
| MS.gene074354 | MS.gene31284 | PPI |
| MS.gene028709 | MS.gene31284 | PPI |
| MS.gene75680 | MS.gene31284 | PPI |
| MS.gene31284 | MS.gene058936 | PPI |
| MS.gene028711 | MS.gene31284 | PPI |
| MS.gene77522 | MS.gene31284 | PPI |
| MS.gene045369 | MS.gene31284 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31284.t1 | MTR_3g084140 | 95.890 | 73 | 3 | 0 | 1 | 73 | 1 | 73 | 5.55e-47 | 144 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31284.t1 | AT3G45180 | 93.151 | 73 | 5 | 0 | 1 | 73 | 1 | 73 | 4.11e-46 | 141 |
| MS.gene31284.t1 | AT3G45180 | 93.151 | 73 | 5 | 0 | 1 | 73 | 1 | 73 | 4.11e-46 | 141 |
| MS.gene31284.t1 | AT5G42300 | 93.151 | 73 | 5 | 0 | 1 | 73 | 1 | 73 | 1.30e-45 | 140 |
Find 12 sgRNAs with CRISPR-Local
Find 13 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACAAAATTCGGATTCAGAAA+TGG | 0.431017 | 2.4:-7940300 | MS.gene31284:CDS |
| CATCGGTGACTTGAAGAAAC+TGG | 0.443655 | 2.4:-7940350 | MS.gene31284:CDS |
| GTACAAGACCTGACAAAATT+CGG | 0.445316 | 2.4:-7940312 | MS.gene31284:CDS |
| AAACTGGTTGCTGCACAAAC+AGG | 0.465046 | 2.4:-7940334 | MS.gene31284:CDS |
| GAACGATCGATTAGGGAAGA+AGG | 0.536861 | 2.4:-7940401 | MS.gene31284:CDS |
| TCGATCGAAGAAGATGATAG+AGG | 0.574816 | 2.4:-7940431 | None:intergenic |
| TACGAGATTCATGATGGCAT+GGG | 0.588238 | 2.4:-7940241 | MS.gene31284:CDS |
| TTACGAGATTCATGATGGCA+TGG | 0.604023 | 2.4:-7940242 | MS.gene31284:CDS |
| TCTGATTACGAGATTCATGA+TGG | 0.606480 | 2.4:-7940247 | MS.gene31284:CDS |
| CAGTTTCTTCAAGTCACCGA+TGG | 0.616322 | 2.4:+7940351 | None:intergenic |
| ATCGAAGAAGATGATAGAGG+TGG | 0.670468 | 2.4:-7940428 | None:intergenic |
| TGCAACGAAGATGATACCAT+CGG | 0.675479 | 2.4:-7940367 | MS.gene31284:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ACAAAATTCGGATTCAGAAA+TGG | - | chr2.4:7940337-7940356 | MS.gene31284:CDS | 30.0% | |
| GTACAAGACCTGACAAAATT+CGG | - | chr2.4:7940325-7940344 | MS.gene31284:CDS | 35.0% | |
| TCTGATTACGAGATTCATGA+TGG | - | chr2.4:7940390-7940409 | MS.gene31284:CDS | 35.0% | |
| ! | TTCTGAATCCGAATTTTGTC+AGG | + | chr2.4:7940336-7940355 | None:intergenic | 35.0% |
| !!! | TGGTTTTGAACGATCGATTA+GGG | - | chr2.4:7940229-7940248 | MS.gene31284:CDS | 35.0% |
| TACGAGATTCATGATGGCAT+GGG | - | chr2.4:7940396-7940415 | MS.gene31284:CDS | 40.0% | |
| TGCAACGAAGATGATACCAT+CGG | - | chr2.4:7940270-7940289 | MS.gene31284:CDS | 40.0% | |
| TTACGAGATTCATGATGGCA+TGG | - | chr2.4:7940395-7940414 | MS.gene31284:CDS | 40.0% | |
| !!! | GTGGTTTTGAACGATCGATT+AGG | - | chr2.4:7940228-7940247 | MS.gene31284:CDS | 40.0% |
| AAACTGGTTGCTGCACAAAC+AGG | - | chr2.4:7940303-7940322 | MS.gene31284:CDS | 45.0% | |
| CAGTTTCTTCAAGTCACCGA+TGG | + | chr2.4:7940289-7940308 | None:intergenic | 45.0% | |
| CATCGGTGACTTGAAGAAAC+TGG | - | chr2.4:7940287-7940306 | MS.gene31284:CDS | 45.0% | |
| GAACGATCGATTAGGGAAGA+AGG | - | chr2.4:7940236-7940255 | MS.gene31284:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.4 | gene | 7940219 | 7940440 | 7940219 | ID=MS.gene31284 |
| chr2.4 | mRNA | 7940219 | 7940440 | 7940219 | ID=MS.gene31284.t1;Parent=MS.gene31284 |
| chr2.4 | exon | 7940219 | 7940440 | 7940219 | ID=MS.gene31284.t1.exon1;Parent=MS.gene31284.t1 |
| chr2.4 | CDS | 7940219 | 7940440 | 7940219 | ID=cds.MS.gene31284.t1;Parent=MS.gene31284.t1 |
| Gene Sequence |
| Protein sequence |
